By Measuring A Physical Property (e.g., Mass, Etc.) Patents (Class 506/12)
  • Publication number: 20130116137
    Abstract: A disclosed chemical detection system for detecting a target material, such as an explosive material, can include a cantilevered probe, a probe heater coupled to the cantilevered probe, and a piezoelectric element disposed on the cantilevered probe. The piezoelectric element can be configured as a detector and/or an actuator. Detection can include, for example, detecting a movement of the cantilevered probe or a property of the cantilevered probe. The movement or a change in the property of the cantilevered probe can occur, for example, by adsorption of the target material, desorption of the target material, reaction of the target material and/or phase change of the target material. Examples of detectable movements and properties include temperature shifts, impedance shifts, and resonant frequency shifts of the cantilevered probe. The overall chemical detection system can be incorporated, for example, into a handheld explosive material detection system.
    Type: Application
    Filed: July 2, 2012
    Publication date: May 9, 2013
    Applicant: Board of Regents of the Nevada System of Higher Education, on behalf of the University of Nevada
    Inventors: Jesse D. Adams, Todd A. Sulchek, Stuart C. Feigin
  • Publication number: 20130109592
    Abstract: Methods to determine the absence or presence of one or more cancer types in an animal are disclosed herein. In some embodiments, amounts of lipids in a sample (e.g., a bodily fluid or treatment thereof) from the animal are used with a predictive model to make the determination. The lipid amounts can be measured, in some instances, using a mass spectrometry system.
    Type: Application
    Filed: June 22, 2011
    Publication date: May 2, 2013
    Inventors: Teresa Whei-Mei Fan, Andrew Nicholas Lane, Richard M. Higashi, Michael Bousamra
  • Publication number: 20130096030
    Abstract: The present invention relates to a multisensor array for detection of analytes in the gas phase or in the liquid phase, comprising at least two different chemo-selective compounds represented by the general formula (I) wherein the hetero atoms X1-X4 and the substituents R1-R4 are defined in the specification and the dashed bonds represent independently of each other either a single bond or a double bond. Said chemo-selective compounds are capable of individually changing physicochemical properties when exposed to analytes or analyte mixtures and these changes can be detected by a transducer or an array of transducers. The present invention does also relate to the use of at least two different chemo-selective compounds in a sensor array, a method for preparation of such sensor arrays and the use of said sensor arrays. Furthermore the present invention relates to methods for detecting and identifying analytes or mixtures thereof in the gas phase or in the liquid phase.
    Type: Application
    Filed: March 31, 2011
    Publication date: April 18, 2013
    Inventors: Jan Oskar Jeppesen, Natalie Kostesha, Carsten Johnsen, Kent Albin Nielsen, Anja Boisen, Mogens Havsteen Jakobsen
  • Publication number: 20130079247
    Abstract: A slotted pin tool, a delivery system containing the pin tool, a substrate for use in the system and methods using the pin tool and system are provided. The slotted pin tool contains a plurality of pins having slotted ends designed to fit around each loci of material deposited on a surface, such as a microarray, without contacting any of the deposited material. Sample is delivered by contacting the pin tool with the surface; the amount delivered is proportional to the velocity of the pin tool as it contacts the surface or the velocity of the liquid when movement of the pin is halted.
    Type: Application
    Filed: November 21, 2012
    Publication date: March 28, 2013
    Applicant: SEQUENOM, INC.
    Inventor: SEQUENOM, INC.
  • Publication number: 20130064799
    Abstract: The present invention is based on the seminal discovery that several kinases play important roles in barrier pathways in somatic cell reprogramming. The present invention provides that modulating expression or activity of these kinases can significantly promote or enhance cell reprogramming efficiency. Key kinases are identified and key regulation networks involving such kinases are also identified that may be advantageously targeted to significantly increase reprogramming efficiency as well as direct differentiation of induced pluripotent stem (iPS) cells.
    Type: Application
    Filed: May 31, 2012
    Publication date: March 14, 2013
    Inventor: Tariq M. Rana
  • Publication number: 20130065769
    Abstract: Disclosed herein are biomarkers related to gastric cancer. The presently identified salivary biomarkers create the basis for a gastric cancer detection bioassay with sensitivity and specificity. Means and methods for evaluating the data generated using multiple biomarkers in order to validate findings and further use of the multiplexed gastric cancer assay in clinical, diagnostic and therapeutic uses is also included.
    Type: Application
    Filed: September 7, 2012
    Publication date: March 14, 2013
    Applicant: The Regents of the University of California
    Inventor: David T. WONG
  • Publication number: 20130059758
    Abstract: The present invention provides a method for detecting and grading benign and malignant tumors using at least one sensor of conductive nanoparticles capped with an organic coating in conjunction with a learning and pattern recognition algorithm. The method utilizes a plurality of response induced parameters to obtain improved sensitivity and selectivity for diagnosis, prognosis, monitoring and staging various types of cancers, or for identifying or grading benign or malignant tumors.
    Type: Application
    Filed: May 22, 2011
    Publication date: March 7, 2013
    Inventors: Hossam Haick, Gregory Shuster
  • Publication number: 20130059743
    Abstract: A microarray with optically recorded information and a sample capable of producing a signal as a response to external influence, or a precursor which, when activated or combined with a reagent, produces a sample capable of generating a signal. The microarray is compatible with a dual functionality optical drive. A method for acquiring information about a sample comprises directing a probe to a sample at a microarray to produce a signal from the sample, wherein the microarray is compatible with a dual functionality optical drive, and detecting the signal. The information optically recorded on the microarray can be in the CD, DVD or HD DVD or Blue Ray format.
    Type: Application
    Filed: February 29, 2012
    Publication date: March 7, 2013
    Inventor: Alexey V. ELISEEV
  • Publication number: 20130059750
    Abstract: The invention relates to a method of diagnosing or monitoring schizophrenia or other psychotic disorder.
    Type: Application
    Filed: January 28, 2011
    Publication date: March 7, 2013
    Inventors: Sabine Bahn, Marlis Huebner
  • Publication number: 20130052172
    Abstract: The present disclosure defines a method for identifying and/or treating risk and/or occurrence of cardiac defect. As is shown herein, microbiomes are reproducibly and detectably associated with cardiac defect risk factors and changes to the microbiome can directly alter cardiac defect risk. The present disclosure demonstrates that microbial signatures can be used to characterize components of microbiomes that associate with altered risk or occurrence of cardiac defects and to identify treatments to reduce risk or severity of cardiac defects.
    Type: Application
    Filed: April 7, 2012
    Publication date: February 28, 2013
    Inventor: John Edward Baker
  • Publication number: 20130053263
    Abstract: MicroRNAs (miRNA) are a recently discovered class of small non-coding RNAs (17-14 nucleotides). Due to their function as regulators of gene expression they play a critical role both in physiological and in pathological processes, such as cancer. The present invention provides novel methods for diagnosing a state of health based on the determination of specific miRNAs that have altered expression levels in different conditions, e.g. disease states compared to healthy controls.
    Type: Application
    Filed: December 30, 2010
    Publication date: February 28, 2013
    Inventors: Andreas Keller, Eckart Meese, Anne Borries, Markus Beier
  • Publication number: 20130045873
    Abstract: The present invention relates generally to methods for identifying organ-specific secreted proteins and for identifying organ-specific molecular blood fingerprints therefrom. As such, the present invention provides compositions comprising such proteins, detection reagents for detecting such proteins, and panels, and arrays for determining organ-specific molecular blood fingerprints.
    Type: Application
    Filed: August 8, 2012
    Publication date: February 21, 2013
    Applicant: Institute For Systems Biology
    Inventors: Leroy HOOD, Biaoyang LIN
  • Publication number: 20130040857
    Abstract: Methods for interpretation of mass spectrometric tests for clinical biomarkers in which the amounts of internal standards are set to equal clinical evaluation thresholds, and preparations for adding stable isotope labeled peptide species to sample digests while minimizing losses and alterations in peptide stoichiometry.
    Type: Application
    Filed: March 15, 2011
    Publication date: February 14, 2013
    Inventor: Norman Leigh Anderson
  • Publication number: 20130039887
    Abstract: Disclosed herein are methods for identifying compounds for the treatment of viral infection, including RNA viral infection and uses of the compounds as pharmaceutical compositions. The identified compounds modulate the RIG-I pathway in vertebrate cells.
    Type: Application
    Filed: April 20, 2011
    Publication date: February 14, 2013
    Applicant: KINETA, INC.
    Inventors: Shawn P. Iadonato, Kristin Bedard, Michael Gale
  • Publication number: 20130040848
    Abstract: The invention relates to a method of detecting a structural change in a molecule, said molecule being attached to a surface, said surface being electrically conductive, wherein the phase of the electrochemical impedance at said surface is monitored, and wherein a change in the phase in the electrochemical impedance at said surface indicates a change in the structure of said molecule. The invention further relates to methods for making arrays having molecules such as, polypeptides attached to electrically conductive surfaces such as electrodes, and to arrays.
    Type: Application
    Filed: September 28, 2012
    Publication date: February 14, 2013
    Inventors: University of Leeds, Medical Research Council
  • Publication number: 20130035251
    Abstract: MicroRNAs (miRNA) are a recently discovered class of small non-coding RNAs (17-14 nucleotides). Due to their function as regulators of gene expression they play a critical role both in physiological and in pathological processes, such as cancer. The present invention provides novel methods for diagnosing a state of health based on the determination of specific miRNAs that have altered expression levels in different conditions, e.g. disease states compared to healthy controls.
    Type: Application
    Filed: December 30, 2010
    Publication date: February 7, 2013
    Applicant: febit holding GmbH
    Inventors: Andreas Keller, Eckart Meese, Anne Borries, Markus Beier
  • Publication number: 20130029857
    Abstract: A method of identifying alleles of polymorphic sites in a plurality of nucleic acid samples including the steps of determining a source tag sharing number ā€œdā€ for each of the alleles; performing a first reaction in a plurality of pools of the alleles to be identified to produce reaction products including a source tag identifying said each pool; pooling the pools to provide pooled pools; for each of the alleles to be identified, performing a second reaction using said reaction products to produce allele-specific second reaction products comprising a marker tag and a derived source tag; identifying said allele-specific second reaction products to identify the alleles. If ā€œdā€ is equal to or larger than a maximum pool size, the first reaction may not be performed. Alleles may be binned together. A microparticle comprising one or more capture probes each comprising an oligonucleotide complementary to a subsequence of a target polynucleotide.
    Type: Application
    Filed: July 25, 2011
    Publication date: January 31, 2013
    Applicant: Bioinventors & Entrepreneurs Network, LLC
    Inventor: Michael Seul
  • Publication number: 20130023444
    Abstract: Provided herein are novel reagents and their use in color-producing detection systems for performing diagnostic tests and analytical assays.
    Type: Application
    Filed: May 23, 2012
    Publication date: January 24, 2013
    Applicant: Diagnostic Innovations, LLC
    Inventor: Victor Kovalenko
  • Publication number: 20130017970
    Abstract: The invention relates to a method of diagnosing or monitoring schizophrenia or other psychotic disorder.
    Type: Application
    Filed: December 20, 2010
    Publication date: January 17, 2013
    Applicant: Psynova Neurotech Ltd.
    Inventors: Sabine Bahn, Yishai Levin, Emanuel Schwarz
  • Publication number: 20130012414
    Abstract: The invention describes methods that use nanostructures and quantum confinement to detect and manipulate chemical and biochemical molecules. To increase selectivity and sensitivity nanostructures are built to have the density of states similar to that in analyte that will be detected and operated. Using device that incorporates such nanostructures, measuring electrical and optical properties of nanostructures and analyte or charge and energy transfer between the nanostructures and analyte a rapid and sensitive continuous detection in fluids can be achieved. Detection can be further enhanced by controlling external environmental parameters and applying external fields. In addition to be detected analyte can be positioned, moved, separated, extracted, and controlled.
    Type: Application
    Filed: September 10, 2012
    Publication date: January 10, 2013
    Inventors: Branislav Vlahovic, Vanja Vlahovic Malloy
  • Publication number: 20120329676
    Abstract: A high throughput screening system and method of an acid-producing microorganism using a mixture of at least two pH indicators are provided. The method may be useful in determining a production amount of an acid, which is a final metabolite secreted by the microorganism, more accurately, rapidly and easily.
    Type: Application
    Filed: February 22, 2012
    Publication date: December 27, 2012
    Inventors: Hwa Young CHO, Byung Jo YU, Jae Chan PARK, Sung Min PARK, Hyun Min KOO, Ju Young LEE
  • Patent number: 8334238
    Abstract: The invention relates to novel methods of detecting alterations in cell cycle regulation in a cell or a cell population and screening for agents capable of modulating cell cycle regulation through the use of multiparameter assays and a fluorescence-activated cell sorter (FACS) machine.
    Type: Grant
    Filed: April 16, 1999
    Date of Patent: December 18, 2012
    Assignee: Rigel, Inc.
    Inventors: Joseph Fisher, James Lorens, Donald Payan, Alexander Rossi
  • Publication number: 20120308650
    Abstract: Covalently modified alginate polymers, possessing enhanced biocompatibility and tailored physiochemical properties, as well as methods of making and use thereof, are disclosed herein. The covalently modified alginates are useful as a matrix for the encapsulation and transplantation of cells. Also disclosed are high throughput methods for the characterizing the biocompatibility and physiochemical properties of modified alginate polymers.
    Type: Application
    Filed: June 4, 2012
    Publication date: December 6, 2012
    Inventors: Arturo J. Vegas, Minglin Ma, Kaitlin M. Bratlie, Daniel G. Anderson, Robert S. Langer
  • Patent number: 8318896
    Abstract: A method for multi-dimensional chromatography of a polyolefin polymer, comprising introducing a solution of the polyolefin polymer into a liquid flowing through a first liquid chromatography stationary phase or a field flow fractionation device and subsequently flowing the solution through a second liquid chromatography stationary phase, the second liquid chromatography stationary phase comprising graphitic carbon, the polyolefin polymer emerging from the liquid chromatography stationary phase with a retention factor greater than zero.
    Type: Grant
    Filed: December 21, 2009
    Date of Patent: November 27, 2012
    Assignee: Dow Global Technologies LLC
    Inventors: William L. Winniford, Rongjuan Cong, Theodore M. Stokich, Jr., Randy J. Pell, Matthew D. Miller, Abhishek Roy, Freddy Van Damme, Alexander W. Degroot, John W. Lyons, David M. Meunier
  • Publication number: 20120283137
    Abstract: A reactive mass label for labelling a biological molecule for detection by mass spectrometry, which label comprises a reactive functionality for labelling thiol groups or carbonyl groups. Also provided is a reactive mass label for labelling a biological molecule for detection by mass spectrometry, wherein the mass label comprises the following structure: X-L-M-S-Re wherein X is a mass marker moiety, L is a cleavable linker, M is a mass normalization moiety, S is a mass series modifying group comprising the following group: wherein J is C?O, K is NH, and n is 2 or J and K are both CH2 and n is 1, and wherein m is at least 1; and Re is a reactive functionality for attaching the mass label to a biological molecule.
    Type: Application
    Filed: September 8, 2010
    Publication date: November 8, 2012
    Inventors: Christian Baumann, Stefan Kienle, Karsten Kuhn, Harald Legner
  • Publication number: 20120277119
    Abstract: Provided are compositions and processes that utilize genomic regions differentially methylated between a mother and her fetus to separate, isolate or enrich fetal nucleic acid from a maternal sample. The compositions and processes described herein are useful for non-invasive prenatal diagnostics, including the detection of chromosomal aneuplodies.
    Type: Application
    Filed: June 13, 2012
    Publication date: November 1, 2012
    Applicant: SEQUENOM, INC.
    Inventors: Mathias EHRICH, Anders Olof Herman Nygren
  • Publication number: 20120238472
    Abstract: The invention relates to a light source for irradiating molecules present in a detection volume with one or more selected wavelengths of light and directing the fluorescence, absorbance, transmittance, scattering onto one or more detectors. Molecular interactions with the light allow for the identification and quantitation of participating chemical moieties in reactions utilizing physical or chemical tags, most typically fluorescent and chromophore labels. The invention can also use the light source to separately and simultaneously irradiate a plurality of capillaries or other flow confining structures with one or more selected wavelengths of light and separately and simultaneously detect fluorescence produced within the capillaries or other flow confining structures. In various embodiments, the flow confining structures can allow separation or transportation of molecules and include capillary, micro bore and milli bore flow systems.
    Type: Application
    Filed: June 1, 2012
    Publication date: September 20, 2012
    Applicant: LUMENCOR, INC.
    Inventors: Claudia B. Jaffe, Steven M. Jaffe, Michieal L. Jones
  • Publication number: 20120238471
    Abstract: Systems and methods biochemically sense a concentration of a ligand using a sensor having a substrate having a metallic nanoparticle array formed onto a surface of the substrate. A light source is incident on the surface. A matrix is deposited over the nanoparticle array and contains a protein adapted to binding the ligand. A detector detects s-polarized and p-polarized light from the reflective surface. Spacing of nanoparticles in the array and wavelength of light are selected such that plasmon resonance occurs with an isotropic point such that ?s and ?p polarizations of the incident light result in substantially identical surface Plasmon resonance, wherein binding of the ligand to the protein shifts the resonance such that differences between the ?S and ?P polarizations give in a signal indicative of presence of the ligand.
    Type: Application
    Filed: March 16, 2012
    Publication date: September 20, 2012
    Inventor: Anatoliy Pinchuk
  • Publication number: 20120231973
    Abstract: An analytical system-on-a-chip can be used as an analytical imaging device, for example, for detecting the presence of a chemical compound. A layer of analytical material is formed on a transparent layer overlying a solid state image sensor. The analytical material can react in known ways with at least one reactant to block light or to allow light to pass through to the array. The underlying sensor array, in turn, can process the presence, absence or amount of light into a digitized signal output. The system-on-a-chip may also include software that can detect and analyze the output signals of the device.
    Type: Application
    Filed: May 24, 2012
    Publication date: September 13, 2012
    Inventor: Terry Gilton
  • Publication number: 20120225954
    Abstract: The disclosure includes a method of screening for, diagnosing or detecting non-small cell lung carcinoma or an increased likelihood of developing non-small cell lung carcinoma in a subject. The method comprises: (a) determining the level of at least one biomarker in a test sample from the subject wherein the at least one biomarker is selected from the biomarkers set out in Table 2, 4A, 4B, 6 and/or 7; and (b) comparing the level of the at least one biomarker in the test sample with a control; wherein detecting a difference in the level of the at least one biomarker in the test sample compared to the control is indicative of whether the subject has or does not have non-small cell lung carcinoma or an increased likelihood of developing non-small cell lung carcinoma.
    Type: Application
    Filed: September 1, 2011
    Publication date: September 6, 2012
    Inventors: Michael F. Moran, Thomas Kislinger, Ming-Sound Tsao, Yuhong Wei, Jiefei Tong, Paul Taylor
  • Publication number: 20120225798
    Abstract: The present invention is directed to methods of detecting nucleic acids in a biological sample. The method is based on a novel combination of a base extension reaction, which provides excellent analytical specificity, and a mass spectrometric analysis, which provides excellent specificity. The method can be used, for example, for diagnostic, prognostic and treatment purposes. The method allows accurate detection of nucleic acids that are present in very small amounts in a biological sample. For example, the method of the present invention is preferably used to detect fetal nucleic acid in a maternal blood sample; circulating tumor-specific nucleic acids in a blood, urine or stool sample; and donor-specific nucleic acids in transplant recipients. In another embodiment, one can detect viral, bacterial, fungal, or other foreign nucleic acids in a biological sample.
    Type: Application
    Filed: February 8, 2012
    Publication date: September 6, 2012
    Inventors: Charles R. Cantor, Chunming Ding, Yuk Ming Dennis Lo, Rossa Wai Kwum Chiu
  • Publication number: 20120220491
    Abstract: Analytes in a sample are resolved by retentate chromatography in a procedure involving adsorbing the analytes on a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.
    Type: Application
    Filed: August 17, 2011
    Publication date: August 30, 2012
    Applicant: Bio-Rad Laboratories, Inc.
    Inventors: T. William Hutchens, Tai-Tung Yip
  • Publication number: 20120214831
    Abstract: To provide a marker for determining sensitivity of a patient to an anti-cancer agent, and novel cancer therapeutic means employing the marker.
    Type: Application
    Filed: October 29, 2010
    Publication date: August 23, 2012
    Inventors: Yusuke Tanigawara, Sayo Suzuki, Yusuke Ikoma, Akito Nishimuta, Tetsuya Suzuki, Shinji Sugimoto
  • Publication number: 20120214685
    Abstract: Methods and compositions are provided for diagnosing ectopic pregnancy in a mammalian subject by detecting changes in expression of ISM2, ADAM12, PST1, PSG7, PST11, PSG9, PSG2 and other genes identified therein, including combinations thereof. A selected gene, gene transcript or protein/peptide expression product, or profiles or signatures formed by combinations of same, detected in a biological fluid of a subject, enables comparison of the corresponding genes, proteins or profiles from that of a reference or control having a normal intrauterine pregnancy. Detection of characteristic changes in the gene profile or protein expression signature of the subject is correlated with a diagnosis of ectopic pregnancy. Various compositions for use in such diagnosis include PCR primer-probe sets or ligands, labeled or immobilized, which are capable of detecting the changes in expression or translation of these targets.
    Type: Application
    Filed: February 15, 2012
    Publication date: August 23, 2012
    Applicants: The Trustees of the University of Pennsylvania, The Wistar Institute
    Inventors: David W. Speicher, Kurt T. Barnhart, Lynn A. Beer
  • Publication number: 20120208722
    Abstract: Surface enhanced Raman spectroscopy (SERS) platforms and methods of making and using such platforms are disclosed herein. Such platforms can be made by immobilizing a biomaterial (e.g., a carbohydrate such as a glycan) by reacting an azide-functional group attached to a surface of a solid substrate with at least one cyclooctyne (e.g., a dibenzocyclooctyne) having a biomaterial or biomaterial binding group attached thereto. In certain embodiments the immobilized biomaterial can be detected using, for example, surface enhanced Raman spectroscopy.
    Type: Application
    Filed: October 19, 2011
    Publication date: August 16, 2012
  • Publication number: 20120202709
    Abstract: Devices and methods for producing and analyzing microarrays are disclosed. In an embodiment, a method for converting a library of beads to an array of analytes includes positioning a plurality of beads having one or more analytes bound therein on a solid support in a spatially separated manner, causing the analytes to be released from the plurality of microparticles, and localizing the released analytes in discrete spots.
    Type: Application
    Filed: February 9, 2012
    Publication date: August 9, 2012
    Applicant: Adeptrix Corp.
    Inventor: Vladislav B. Bergo
  • Publication number: 20120202708
    Abstract: Provided herein is a variant in the RCA locus and methods for detecting the presence, absence or amount of multiple forms of the variant.
    Type: Application
    Filed: October 13, 2011
    Publication date: August 9, 2012
    Applicant: SEQUENOM, INC.
    Inventors: Lorah T. Perlee, Paul A. Oeth, Michael R. Barnes
  • Publication number: 20120196770
    Abstract: Provided herein are methods and compositions useful for detecting the production of industrially useful compounds (e.g., isoprenoids, polyketides, and fatty acids) in a cell, for example, a microbial cell genetically modified to produce one or more such compounds. In some embodiments, the methods comprise encapsulating the cell in a hydrogel particle, and detecting the compound within the hydrogel particle.
    Type: Application
    Filed: January 27, 2012
    Publication date: August 2, 2012
    Applicant: Amyris, Inc.
    Inventor: Jeremy Agresti
  • Publication number: 20120190584
    Abstract: The chip includes electrodes on a substrate. The electrodes include a working electrode, a reference electrode, and a counter electrode. The reference electrode is constructed so as to not have an intrinsic potential. A self-assembly monolayer is positioned on the reference electrode. The self-assembly monolayer includes spacers and active probes. The active probes are configured to have a higher affinity for a capture probe than the spacers have for the capture probe.
    Type: Application
    Filed: May 28, 2008
    Publication date: July 26, 2012
    Inventor: Jen-Jr Gau
  • Patent number: 8227252
    Abstract: The invention relates to methods for multiplex analysis of complex protein samples from plants using mass spectroscopy. In some embodiments, the disclosure concerns methods for maintaining a transgenic plant variety, for example by analyzing generations of a transgenic plant variety for presence and concentration of multiplexed transgenic proteins.
    Type: Grant
    Filed: June 3, 2010
    Date of Patent: July 24, 2012
    Assignee: Dow AgroSciences, LLC
    Inventors: John R. Lawry, Joshua A. Flook
  • Publication number: 20120184460
    Abstract: Methods of evaluating a gene-regulatory element, including libraries of known or candidate gene-regulatory elements, are provided. Aspects of the methods include cytometrically analyzing a cell comprising a gene-regulatory element construct, e.g., a plasmid, having an activity reporter comprising a first signal reporter domain which produces a first signal operatively coupled to a gene-regulatory element of interest; and a noise reporter comprising a second signal reporter which produces a second signal that is distinguishable from the first signal to obtain the first and second signals. The first signal is then normalized with the second signal to obtain a normalized activity signal, which normalized activity signal is then employed to evaluate the gene-regulatory element. Also provided are reagents, systems and kits that find use in practicing methods of the invention.
    Type: Application
    Filed: January 13, 2012
    Publication date: July 19, 2012
    Inventors: Joe C. Liang, Christina D. Smolke
  • Publication number: 20120172255
    Abstract: Herein is reported a method for the determination of the glycosylation of an immunoglobulin with electrospray mass spectrometry but without the need for a chromatographic purification step after the digestion of the immunoglobulin and prior to the mass spectrometric analysis.
    Type: Application
    Filed: September 3, 2010
    Publication date: July 5, 2012
    Applicant: HOFFMANN-LA ROCHE, INC,
    Inventors: Markus Haberger, Dietmar Reusch, Maurice Selman, Manfred Wuhrer
  • Publication number: 20120172256
    Abstract: The invention provides a liquid cell for an atomic force microscope. The liquid cell includes a liquid cell housing with an internal cavity to contain a fluid, a plurality of conductive feedthroughs traversing the liquid cell housing between the internal cavity and a dry side of the liquid cell, a cantilevered probe coupled to the liquid cell housing, and a piezoelectric drive element disposed on the cantilevered probe. The cantilevered probe is actuated when a drive voltage is applied to the piezoelectric drive element through at least one of the conductive feedthroughs. A method of imaging an object in a liquid medium and a method of sensing a target species with the liquid cell are also disclosed.
    Type: Application
    Filed: March 12, 2012
    Publication date: July 5, 2012
    Applicants: Reno
    Inventors: Jesse D. Adams, Benjamin S. Rogers, Todd A. Sulchek
  • Publication number: 20120149585
    Abstract: A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.
    Type: Application
    Filed: February 17, 2012
    Publication date: June 14, 2012
    Applicant: Illumina, Inc.
    Inventors: Wenyi Feng, Jason Bryant, Dale Buermann
  • Publication number: 20120122731
    Abstract: Screening in a microfluidic device is mediated by a magnetic field that in some manner displaces or otherwise activates the entities of interest. Entities of interest can be identified and/or separated from one or more other components provided to the microfluidic device. Microfluidic devices may have mechanisms that apply a defined magnetic field to a region of the microfluidic device where library members pass through sequentially and/or in parallel.
    Type: Application
    Filed: January 17, 2007
    Publication date: May 17, 2012
    Inventors: Hyongsok Soh, Patrick Sean Daugherty, Sang-Hyun Oh
  • Publication number: 20120121618
    Abstract: The disclosure features methods and compositions for determining whether a male subject has, or is at an increased risk of developing, an aggressive form of prostate cancer. The disclosure also features methods for adjusting a treatment regimen (e.g., discontinuing a therapy comprising an antioxidant) or administering a therapy that does not contain an antioxidant for a male subject in view of one or both of an MnSOD2 genotype status and an elevated level of an antioxidant in a biological sample obtained from the male subject. Also featured are methods for reducing superoxide levels in a subject based on one or both of an MnSOD2 genotype status and an elevated level of an antioxidant in a biological sample obtained from the male subject.
    Type: Application
    Filed: February 12, 2010
    Publication date: May 17, 2012
    Inventors: Philip W. Kantoff, William K. Oh, June M. Chan, Wanling Xie, Miyako Abe, Lorelei A. Mucci
  • Publication number: 20120115755
    Abstract: A microfluidic device may employ one or more sorting stations for separating target species from other species in a sample. The separation is driven by magnetophoresis. A sorting station generally includes separate buffer and sample streams. A magnetic field gradient applied to the sorting station deflects the flow path of magnetic particles (which selectively label the target species) from a sample stream into a buffer stream. The buffer stream leaving the sorting station is used to detect or further process purified target species labeled with the magnetic particles.
    Type: Application
    Filed: October 28, 2011
    Publication date: May 10, 2012
    Inventors: Sang-Hyun Oh, Amarendra Kumar Singh, Yanting Zhang, Dharmakeerthi Nawarathna, Unyoung Kim, Patrick Sean Daugherty, Hyongsok Soh, Brian Scott Ferguson
  • Publication number: 20120108465
    Abstract: A sensor array is provided including a plurality of fibers being woven to form 3-D periodic fiber structures. A selective number of the fibers include gaseous sensing materials to detect selective gases. A plurality of spacing elements provides adequate spacing between successively arranged nano-fibers. The nano-fibers and spacing elements are arranged to form a 3-D scaffolding structure for detecting specific or combinations of gaseous analytes.
    Type: Application
    Filed: September 28, 2009
    Publication date: May 3, 2012
    Inventors: Eric B. Duoss, Jennifer A. Lewis, Avner Rothschild, Kathy Sahner, Harry L. Tuller
  • Patent number: 8168568
    Abstract: A method for selecting combinations of drugs for treatment of diseases that arise from deranged signaling pathways is disclosed. The method involves measuring the activity states for signaling proteins in a diseased cell and determining whether the activity states are different from the activity states observed for a reference cell such as a normal cell. Based on the observed differences, combinations of two or more drugs are selected to reduce these differences. Treatment of a subject with the combinations restores the activity states of the signaling proteins of the deranged disease-associated signaling pathways toward the activity states observed in the reference cell. Since the diseased cell and the reference cell can both be obtained from the same subject, combinations of drugs that specifically target patient-specific signaling derangements is possible.
    Type: Grant
    Filed: October 16, 2009
    Date of Patent: May 1, 2012
    Assignee: The United States of America, as represented by the Secretary of the Department of Health and Human Services
    Inventors: Arpita I. Mehta, Lance A. Liotta, Emanuel F. Petricoin
  • Publication number: 20120101007
    Abstract: A sensor for detecting of an analyte in a solution phase comprises a plurality of functionalised silver nanoplates wherein a functionalising agent is directly bonded to the surfaces of the nanoplates. The nanoplates provide a detectable wavelength shift change in their local surface plasmon resonance spectrum in response to the binding of an analyte. Two or more of the nanoplates may be electromagnetically coupled.
    Type: Application
    Filed: April 8, 2010
    Publication date: April 26, 2012
    Inventors: Damian Ahern, Margaret Brennan Fournet, Denise Charles, Stephen Michael Cunningham, Patrick Fournet, John Moffat Kelly, Deirdre Marie Ledwith, Muriel Celine Voisin