Encodes An Enzyme Patents (Class 536/23.2)
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Publication number: 20140344997Abstract: Disclosed are polynucleotides encoding polypeptides that comprise the biosynthetic pathway for lignin in the jute plant. The present invention relates generally to the field of plant lignin biosynthesis genes, polypeptides encoded by such genes, and the use of such polynucleotide and polypeptide sequences for controlling plant lignin production. Also disclosed are methods for using the polynucleotides and polypeptides to influence the quality and amount of fiber produced by jute.Type: ApplicationFiled: April 25, 2012Publication date: November 20, 2014Applicant: Bangladesh Jute Research InstituteInventors: Maqsudul Alam, Haseena Khan, Mahboob Zaman, Mohammed K. Uddin, Mohammed S. Haque, Mohammed S. Islam, Muhammed S. Azam
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Publication number: 20140341977Abstract: The present disclosure provides compositions and methods for the prevention or treatment of ocular neovascularization, such as AMD, in a human subject, by administering subretinally a pharmaceutical composition comprising a pharmaceutically effective amount of a vector comprising a nucleic acid encoding soluble Fms-related tyrosine kinase-1 (sFlt-1) protein to the human subject.Type: ApplicationFiled: May 19, 2014Publication date: November 20, 2014Applicant: Avalanche Australia Pty Ltd.Inventors: Ian J. Constable, Elizabeth P. Rakoczy, Chooi-May Lai, Thomas W. Chalberg, JR.
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Publication number: 20140344996Abstract: This disclosure relates to the field of plant molecular biology and concerns methods for enhancing the abiotic stress tolerance in plants by modulating the expression of a gene involved in the gibberellin biosynthesis during the period of abiotic stress. This disclosure also provides chimeric constructs useful in the methods disclosed herein. In addition, transgenic plants having an enhanced abiotic stress resistance are provided herein.Type: ApplicationFiled: September 14, 2012Publication date: November 20, 2014Applicant: VIB VZWInventors: Dirk Gustaaf Inze, Marieke Dubois, Aleksandra Skirycz
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Publication number: 20140342982Abstract: New gene therapy constructions and compositions are the subject of present invention. The gene therapy compositions consist in adeno-associated vectors which jointly express insulin (Ins) and glucokinase (Gck) genes. The new gene therapy constructions are useful for treatment of diabetes either in dogs or human beings.Type: ApplicationFiled: July 12, 2011Publication date: November 20, 2014Applicant: UNIVERSIDAD AUTÓNOMA DE BARCELONAInventors: Fátima Bosch Tubert, Eduard Ayuso López, David Callejas Castiñeiras
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Patent number: 8889392Abstract: Modified (iso)eugenol 4-O-methyltransferase enzymes having novel capacity for methylation of monolignols and reduction of lignin polymerization in plant cell wall are disclosed. Sequences encoding the modified enzymes are disclosed.Type: GrantFiled: October 28, 2011Date of Patent: November 18, 2014Assignee: Brookhaven Science Associates, LLCInventors: Chang-Jun Liu, Mohammad-Wadud Bhuiya, Kewei Zhang
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Patent number: 8889127Abstract: The present invention relates to compositions and methods for delivering lysosomal proteins. The compositions and methods described herein permit the targeted delivery of exogenous lysosomal proteins to cell surface proteins that allow their internalization via non-clathrin pathways. The present invention further relates to the use of the compositions and methods for enzyme replacement therapy of lysosomal storage diseases. Nucleic acids, recombinant cells and kits useful for making and using the compositions of the invention are also provided.Type: GrantFiled: July 1, 2005Date of Patent: November 18, 2014Assignee: Icahn School of Medicine at Mount SinaiInventors: Silvia Muro Galindo, Vladimir R. Muzykantov, Edward Howard Schuchman
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Patent number: 8889391Abstract: The present disclosure provides novel beta-alanine/alpha ketoglutarate aminotransferase nucleic acid and protein sequences having increased biological activity. Also provided are cells containing such enzymes, as well as methods of their use, for example to produce malonyl semialdehyde and downstream products thereof, such as 3-hydroxypropionic acid and derivatives thereof.Type: GrantFiled: September 12, 2011Date of Patent: November 18, 2014Assignee: Cargill, IncorporatedInventors: Holly Jean Jessen, Hans H. Liao, Steven John Gort, Olga V. Selifonova
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Patent number: 8889385Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.Type: GrantFiled: March 15, 2013Date of Patent: November 18, 2014Assignee: Butamax Advanced Biofuels LLCInventors: Gail K. Donaldson, Andrew C. Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan
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Patent number: 8889387Abstract: Provided herein are improved compositions and methods for the increased production of isoprene. Also provided herein are improved compositions and methods for the increased production of heterologous polypeptides capable of biological activity.Type: GrantFiled: May 6, 2013Date of Patent: November 18, 2014Assignees: Danisco US Inc., The Goodyear Tire & Rubber CompanyInventors: Zachary Q. Beck, Marguerite A. Cervin, Alex T. Nielsen, Caroline M. Peres
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Patent number: 8889844Abstract: A nucleic acid comprising a transcription regulation sequence whose transcription is induced by a trans-acting factor of a human immunodeficiency virus and a gene encoding a polypeptide having an endoribonuclease activity specific to single-stranded RNA, wherein the gene is located in such a position that the expression of the gene can be regulated by the transcription regulation sequence; a method for production of a cell showing an inhibited replication of a human immunodeficiency virus therein, the method comprising the step of introducing the nucleic acid into a cell; and a method for treatment or prevention of a human immunodeficiency virus infection.Type: GrantFiled: August 10, 2006Date of Patent: November 18, 2014Assignee: Takara Bio Inc.Inventors: Hideto Chono, Kazuya Matsumoto, Junichi Mineno, Ikunoshin Kato
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Patent number: 8889709Abstract: The present invention relates in general to an inhibitor of a glutaminyl peptide cyclotransferase-like protein (QPCTL), and the use thereof for the treatment and/or prevention of an inflammatory disease or disorder selected from the group consisting of (a) chronic and acute inflammations, e.g. rheumatoid arthritis, atherosclerosis, restenosis, pancreatitis; (b) other inflammatory diseases, e.g. neuropathic pain, graft rejection/graft failure/graft vasculopathy, HIV infections/AIDS, gestosis, tuberous sclerosis, Guillain-Barré syndrome, chronic inflammatory demyelinizing polyradiculoneuropathy and multiple sclerosis; (c) neuroinflammation; and (d) neurodegenerative diseases, e.g. mild cognitive impairment (MCI), Alzheimer's disease, neurodegeneration in Down Syndrome, Familial British Dementia, and Familial Danish Dementia, which may result from neuroinflammation.Type: GrantFiled: September 4, 2009Date of Patent: November 18, 2014Assignee: Probiodrug AGInventors: Hans-Ulrich Demuth, Stephan Schilling, Michael Wermann, Holger Cynis, Astrid Kehlen, Daniel Friedrich, Torsten Hoffmann, Kathrin Gans, Jens-Ulrich Rahfeld, Ulrich Heiser, Michael Almstetter, Robert Sommer, Ulf-Torsten Gaertner, Antje Hamann, Michael Thormann, Andreas Treml
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Patent number: 8889356Abstract: The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.Type: GrantFiled: February 18, 2014Date of Patent: November 18, 2014Assignees: The Broad Institute Inc., Massachusetts Institute of TechnologyInventor: Feng Zhang
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Patent number: 8889381Abstract: Provided herein are diterpene synthases (diTPS) and methods for producing diterpenoids. Also provided herein are nucleic acid sequences encoding diTPS, diTPS amino acid sequences, diTPS proteins, vectors, cells, transgenic organisms, uses, compositions, methods, processes, and kits thereof.Type: GrantFiled: November 21, 2012Date of Patent: November 18, 2014Assignee: University of British ColumbiaInventors: Joerg Bohlmann, Philipp Zerbe
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Publication number: 20140335574Abstract: A mutant bacterial acetolactate synthase (AHAS I) which is resistant to feedback inhibition by L-valine is described. Also described is a method for producing branched-chain L-amino acids using a bacterium from the Enterobacteriaceae family wherein the L-amino acid productivity of said bacterium is enhanced by the use of the acetolactate synthase (AHAS I) which is resistant to feedback inhibition by L-valine. This acetolactate synthase contains a mutant small subunit encoded by the mutant ilvN gene.Type: ApplicationFiled: July 17, 2014Publication date: November 13, 2014Applicant: AJINOMOTO CO., INC.Inventors: Elena Viktorovna Sycheva, Vsevolod Aleksandrovich Serebryanyy, Tatyana Abramovna Yampolskaya, Ekaterina Sergeevna Preobrazhenskaya, Natalia Viktorovna Stoynova
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Publication number: 20140335591Abstract: Described herein are methods for increasing the amount of protein secreted by a cell. In one case, a cell is provided which contains a heterologous nucleic acid encoding a protein having unfolded protein response modulating activity and a heterologous nucleic acid encoding a protein of interest to be secreted. In one case, the protein having unfolded protein response modulating activity is selected from the proteins selected from the group consisting of HAC1, PTC2 and IRE1. The protein of interest can be any secreted protein such as a therapeutic or an industrial enzyme. For example the protein can be selected from the group consisting of lipase, cellulase, endo-glucosidase H, protease, carbohydrase, reductase, oxidase, isomerase, transferase, kinase, phosphatase, alpha-amylase, glucoamylase, lignocellulose hemicellulase, pectinase and ligninase.Type: ApplicationFiled: May 8, 2014Publication date: November 13, 2014Applicant: DANISCO US INC.Inventors: MERJA E. PENTTILA, MICHAEL WARD, HUAMING WANG, MARI J. VALKONEN, MARKKU L.A. SALOHEIMO
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Publication number: 20140335102Abstract: Methods are disclosed for increasing the binding affinity of binding proteins using in silico affinity maturation.Type: ApplicationFiled: December 18, 2012Publication date: November 13, 2014Applicant: SanofiInventors: Michael Oberlin, Romano Kroemer, Vincent Mikol, Hervé Minoux, Nicolas Baurin
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Publication number: 20140335572Abstract: Provided are isolated polypeptides having catalase activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: December 19, 2012Publication date: November 13, 2014Inventors: Ye Liu, Junxin Duan, Yu Zhang, Lan Tang
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Publication number: 20140335524Abstract: The present invention relates to the identification of alleles of the MET2 and SKP2 genes having the effect of reducing the production of sulphites, of hydrogen sulphide and of acetaldehyde by Saccharomyces, and to the use of these alleles in methods for controlling the production of these compounds during alcoholic fermentation.Type: ApplicationFiled: January 24, 2013Publication date: November 13, 2014Inventors: Bruno Blondin, Jessica Noble, Isabelle Sanchez
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Publication number: 20140335567Abstract: The present disclosure relates to an enzyme derived from Candida bombicola that is capable of lactonizing or polymerizing carbohydrate-containing compounds, lipids, fatty acids, hydroxylated fatty acids, alcohols, dicarboxylic acids or mixtures thereof. Hence, host cells comprising the latter enzyme can be used, via the formation of intra- or inter-molecular ester-bounds, to produce, for example, lactonized sophorolipids or polymers of acidic sophorolipids. On the other hand, host cells having lost their capability to produce a functional enzyme disclosed herein can be used to produce 100% acidic sophorolipids.Type: ApplicationFiled: December 14, 2012Publication date: November 13, 2014Inventors: Inge Van Bogaert, Katarzyna Ciesielska, Bart Devreese, Wim Soetaert, Sophie Roelants
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Publication number: 20140335087Abstract: The present invention provides aspartyl-tRNA synthetase and Fc region conjugate polypeptides (DRS-Fc conjugates), such as DRS-Fc fusion proteins, compositions comprising the same, and methods of using such conjugates and compositions for treating or diagnosing a variety of conditions. The DRS-Fc conjugates of the invention have improved controlled release properties, stability, half-life, and other pharmacokinetic and biological properties relative to corresponding, unmodified DRS polypeptides.Type: ApplicationFiled: December 27, 2012Publication date: November 13, 2014Inventors: Ying Ji Buechler, Chi-Fang Wu, Ryan Andrew Adams, Jeffrey D. Watkins, John D. Mendlein
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Publication number: 20140335553Abstract: Methods are provided measuring L-lysine using a variant enzyme, an L-lysine measurement kit, and an enzyme sensor. Variant L-amino acid oxidase having a predetermined amino acid mutation, and having oxidase activity that is highly substrate-specific for L-lysine; a method for measuring L-lysine using this variant enzyme; an L-lysine measurement kit; and an enzyme sensor are also provided.Type: ApplicationFiled: July 29, 2014Publication date: November 13, 2014Applicants: AJINOMOTO CO., INC., TOYAMA PREFECTUREInventors: Yasuhisa Asano, Daisuke Matsui
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Publication number: 20140336236Abstract: Novel ALK and NTRK1 fusion molecules and uses are disclosed.Type: ApplicationFiled: April 21, 2014Publication date: November 13, 2014Applicant: FOUNDATION MEDICINE, INC.Inventors: Maureen T. Cronin, Doron Lipson, Roman Yelensky
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Publication number: 20140335575Abstract: An object of the present invention is to provide a means for producing an optically active tropic acid that is a compound useful as a synthetic raw material or an intermediate for pharmaceutical products and the like. The present invention provides a novel polypeptide having activity to (R)-selectively hydrolyze a racemic tropic acid amide, DNA encoding the polypeptide, a vector containing the DNA, a transformant prepared by transformation with the vector, and a method for producing an optically active carboxylic acid amide and an optically active carboxylic acid using them.Type: ApplicationFiled: September 28, 2012Publication date: November 13, 2014Inventors: Masutoshi Nojiri, Hiroyuki Kanamaru, Akiko Nishi, Shigeru Kawano, Yoshihiko Yasohara
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Publication number: 20140335579Abstract: The present invention relates to polynucleotides from Cochliobolus heterostrophus C5, Cyanothece sp. CCY0110, Mycocentrospora acerin and Hyaloperonospora parasitica, which code for desaturases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides according to the invention, and to the polypeptides encoded by the polynucleotides. The invention furthermore relates to antibodies against the polypeptides according to the invention. Finally, the invention also relates to production processes for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to their use as drugs, cosmetics, foodstuffs, feedstuffs, preferably fish food, or food supplements.Type: ApplicationFiled: July 23, 2014Publication date: November 13, 2014Inventors: Toralf Senger, Jörg Bauer, Johnathan A. Napier
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Patent number: 8883456Abstract: The invention relates to a process of process of producing a fermentation product, comprising: liquefying a starch-containing material to dextrins with an alpha-amylase in the presence of a GH61 polypeptide; saccharifying the dextrins to a sugar with a glucoamylase; and fermenting the sugar using a fermenting organism.Type: GrantFiled: March 30, 2011Date of Patent: November 11, 2014Assignee: Novozymes North America, Inc.Inventors: Chee-Leong Soong, Jeremy Saunders
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Patent number: 8883458Abstract: The invention relates to a process for preparing L-amino acids by fermenting recombinant microorganisms of the Enterobacteriaceae family, characterized in that a) the desired L-amino acid-producing microorganisms, in which the yjcG-ORF, or nucleotide sequences or alleles encoding the gene product, is/are enhanced, in particular overexpressed, is cultured in a medium under conditions under which the desired L-amino acid is accumulated in the medium or in the cells, and b) the desired L-amino acid is isolated, with, where appropriate, constituents of the fermentation broth, and/or the biomass remaining in its/their entirety or in portions (from ?0 to 100%) in the isolated product or being removed completely.Type: GrantFiled: January 22, 2010Date of Patent: November 11, 2014Assignee: Evonik Degussa GmbHInventor: Nicole Dusch
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Patent number: 8883434Abstract: An object is to provide a novel enzyme that exhibits glucose dehydrogenase activity. Furthermore, another object is to provide a novel method pertaining to enzyme modification.Type: GrantFiled: November 22, 2010Date of Patent: November 11, 2014Assignee: Amano Enzyme Inc.Inventors: Kyoichi Nishio, Satoshi Koikeda
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Patent number: 8883477Abstract: The invention describes novel pharmaceutical compositions for the treatment of virus infections and cancer. The pharmaceutical compositions include mutant oligoadenylate synthetases (OAS) that have either enhanced cell permeability, reduced oxidative potential, improved antiviral activity, improved enzymatic activity, or absent enzymatic activity. The pharmaceutical compositions have improved drug properties and retain or have enhanced antiviral activity relative to their native forms. The pharmaceutical compositions further include chemically modified oligoadenylate synthetases, such chemical modifications being designed to increase serum stability and reduce immunogenicity in vivo. Such chemical modifications further increase drug stability and manufacturability in vitro. Compositions composed of more than ninety novel modifications are described. Also described are antibodies to polypeptides of the invention.Type: GrantFiled: June 14, 2013Date of Patent: November 11, 2014Assignee: Kineta Two, LLCInventors: Shawn P. Iadonato, Charles L. Magness, Mark Branum, Maralee McVean, Christina Scherer
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Patent number: 8883466Abstract: The present invention relates to a bacterial cell of the genus Pasteurella comprising a heterologous polypeptide having formate dehydrogenase activity. The present invention also relates to a method of manufacturing succinic acid and the use of the bacterial cell for the manufacture of succinic acid.Type: GrantFiled: December 21, 2009Date of Patent: November 11, 2014Assignee: BASF SEInventors: Edzard Scholten, Stefan Haefner, Hartwig Schröder
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Patent number: 8883464Abstract: This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes 3-hydroxypropionic acid.Type: GrantFiled: September 27, 2010Date of Patent: November 11, 2014Assignees: OPX Biotechnologies, Inc., The Regents of the University of Colorado, a Body CorporateInventors: Michael D. Lynch, Ryan T. Gill, Tanya E. W. Lipscomb
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Patent number: 8883476Abstract: Provided is a mutant of propionyl-CoA transferase from Clostridium propionicum that can convert lactate into lactyl-CoA with high efficiency in a method of preparing a polylactate (PLA) or PLA copolymer using microorganisms. Unlike conventional propionyl-CoA transferase which is weakly expressed in E. coli, when a mutant of propiony-CoA transferase from Clostridium propionicum is introduced into recombinant E. coli, lactyl-CoA can be supplied very smoothly, thereby enabling highly efficient preparation of polylactate (PLA) and PLA copolymer.Type: GrantFiled: July 26, 2013Date of Patent: November 11, 2014Assignee: LG Chem, Ltd.Inventors: Si Jae Park, Taek Ho Yang, Hye Ok Kang, Sang Hyun Lee, Eun Jung Lee, Tae Wan Kim
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Patent number: 8883416Abstract: The invention is directed to novel reductive dehalogenase genes encoding for reductive dehalogenases which are capable of dehalogenating halogenated organic compounds and may be useful in the bioremediation of pollutants. In particular, the invention provides an isolated polynucleotide of a novel vinyl chloride dehalogenase gene (bvcA). The novel vinyl chloride dehalogenase gene encodes a reductive dehalogenase that is capable of the complete reduction of vinyl chloride to ethene.Type: GrantFiled: September 14, 2005Date of Patent: November 11, 2014Assignee: Georgia Tech Research CorporationInventors: Frank Loeffler, Kirsti M. Ritalahti, Rosa Krajmalnik-Brown, Ivy Thomson
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Patent number: 8883445Abstract: The present invention provides genetically engineered strains of methylotrophic yeast including Pichia and especially Pichia pastoris capable of producing proteins with reduced or modified glycosylation. Methods of producing glycoproteins with reduced and/or modified glycosylation using such genetically engineered strains of Pichia are also provided. Vectors, which comprise coding sequences for ?-1,2-mannosidase I, glucosidase II, GlcNAc-tranferase I and mannosidase II or comprising OCH1 disrupting sequence, for transforming methylotrophic yeasts are contemplated by the present invention. Kit for providing the comtemplated vectors are also included in this invention.Type: GrantFiled: July 13, 2007Date of Patent: November 11, 2014Assignees: Research Corporation Technologies, Inc., Universiteit Gent, Vib, VZWInventors: Roland Contreras, Nico L. M. Callewaert, Steven C. J. Geysens, Vladimir Kaigorodov, Vervecken Wouter
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Patent number: 8883991Abstract: The present invention provides compositions, methods, and kits for covalently linking nucleic acid molecules. The methods include a strand invasion step. For example, a method of covalently linking double stranded (ds) nucleic acid molecules can include contacting a first ds nucleic acid molecule, which has a topoisomerase linked to a 3? terminus of one end and has a single stranded 5? overhang at the same end, with a second ds nucleic acid molecule having a blunt end, such that the 5? overhang can hybridize to a complementary sequence of the blunt end of the second nucleic acid molecule, and the topoisomerase can covalently link the ds nucleic acid molecules. The methods are simpler and more efficient than previous methods for covalently linking nucleic acid sequences, and the compositions and kits facilitate practicing the methods, including methods of directionally linking two or more ds nucleic acid molecules.Type: GrantFiled: April 15, 2013Date of Patent: November 11, 2014Assignee: Life Technologies CorporationInventors: Jonathan Chesnut, Steward Shuman, Knut Madden, John Heyman, Bennett Robert
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Patent number: 8883457Abstract: Described are compositions and methods relating to starch processing without a phytase pretreatment step and without adjustment of the slurry pH adjustment.Type: GrantFiled: January 9, 2013Date of Patent: November 11, 2014Assignee: Danisco US Inc.Inventors: Suzanne Breneman, Thomas S. Green, Michael Jay Pepsin, Vivek Sharma, Jayarama K. Shetty
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Patent number: 8882978Abstract: Disclosed herein are a gene (polynucleotide) encoding an FAD-conjugated glucose dehydrogenase which can be characterized by reactivity to glucose, thermal stability, substrate-recognition performance, and low activity for maltose; a process for the production of the enzyme using a transformant cell transfected with the gene; a method for the determination of glucose; a reagent composition for use in the determination of glucose; and a biosensor for use in the determination of glucose. An embodiment is a polynucleotide encoding an FAD-conjugated glucose dehydrogenase, comprising a polypeptide whose amino acid sequence comprises X1-X2-X3-X4-X5-X6, wherein X1 and X2 independently represent an aliphatic amino acid residue; X3 and X6 independently represent a branched amino acid residue; and X4 and X5 independently represent a heterocyclic amino acid residue or an aromatic amino acid residue.Type: GrantFiled: June 18, 2013Date of Patent: November 11, 2014Assignee: Ikeda Food Research Co., Ltd.Inventors: Takako Yada, Koji Miyamoto, Michinari Honda
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Publication number: 20140329787Abstract: The present invention relates to a novel Phytophthora phospholipase C and uses thereof, methods of identifying modulators and inhibitors of a biological function of the phospholipase C, and methods of inhibiting Phytophthora growth comprising inhibiting a biological function of a novel Phytophthora phospholipase C.Type: ApplicationFiled: October 17, 2012Publication date: November 6, 2014Inventors: Dean Ian Phillips, Peter Luke Beech
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Publication number: 20140328821Abstract: The present invention relates to nucleic acids coding for and capable of expressing a rod-derived cone viability factor (RdCVF) and viral vectors containing these nucleic acids. The invention also relates to compositions and pharmaceutical preparations comprising these nucleic acids or vectors, methods of producing or secreting an RdCVF, and methods of treatment.Type: ApplicationFiled: October 26, 2012Publication date: November 6, 2014Applicant: Wellstat Ophthalmics CorporationInventor: Tianci Luo
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Publication number: 20140329292Abstract: This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to a method to release cellular content by degradation of cell walls. The invention also relates to methods to use the novel enzymes and compositions of such enzymes in a variety of other processes, including washing of clothing, detergent processes, biorefining, deinking and biobleaching of paper and pulp, and treatment of waste streams.Type: ApplicationFiled: September 6, 2013Publication date: November 6, 2014Applicant: DYADIC INTERNATIONAL, INC.Inventors: Mark A. EMARLFARB, Alexander Vasilievich Gusakov, Peter J. Punt, Jan Cornelis Verdoes, Arkady Panteleimonovich Sinitsyn, Elena Vlasenko, Sandra Wihelmina Agnes Hinz, Mark Gosink, Zhijie Jiang
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Publication number: 20140331364Abstract: Provided are isolated polypeptides having beta-glucosidase activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: December 19, 2012Publication date: November 6, 2014Inventors: Ye Liu, Junxin Duan, Yu Zhang, Lan Tang
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Publication number: 20140329284Abstract: The present invention relates to chimeric polypeptides having beta-glucosidase activity.Type: ApplicationFiled: September 28, 2012Publication date: November 6, 2014Inventors: Mark Wogulis, David Osborn, Tia Heu
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Publication number: 20140329291Abstract: Disclosed are methods and compositions related to ONC-T18, D4-desaturases, D5 elongases, their isolation, characterization, production, identification, and use for fatty acid production, as well as organisms containing these compositions and organisms expressing them.Type: ApplicationFiled: May 6, 2014Publication date: November 6, 2014Applicant: DSM Nutritional Products AGInventors: Adam M. Burja, Gabrielle S. Chafe, Helia Radianingtyas
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Publication number: 20140330003Abstract: The present disclosure relates to the isolation, purification, and characterization of a diacylglycerol acyltransferase 2 (DGAT2), and genes encoding DGAT2, from algae. DGAT2 can incorporate very long-chain polyunsaturated fatty acids into triacylglycerol more efficiently than DGAT1. The disclosure concerns methods of regulating seed oil content, fatty acid synthesis and fatty acid composition using the DGAT2 gene and to tissues and plants transformed with the gene. The disclosure also relates to transgenic plants, plant tissues and plant seeds having a genome containing an introduced DNA sequence of the disclosure, and a method of producing such plants and plant seeds.Type: ApplicationFiled: February 24, 2014Publication date: November 6, 2014Applicants: Dow AgroSciences, LLC, National Research Council of CanadaInventors: Jitao Zou, Jingyu Xu, Zhifu Zheng
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Publication number: 20140328927Abstract: The present invention encompasses a composition capable of delivering and expressing a nucleic acid encoding UDP-Glucuronosyltransferases, p53 or a combination thereof into a cell, and methods for treating tumors.Type: ApplicationFiled: July 21, 2014Publication date: November 6, 2014Inventors: Anna Radominska-Pandya, Alexandru S. Biris
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Publication number: 20140328820Abstract: The present invention provides new compositions and methods for preventing and treating pathogen infection. In particular, the present invention provides compounds having an anchoring domain that anchors the compound to the surface of a target cell, and a therapeutic domain that can act extracellularly to prevent infection of a target cell by a pathogen, such as a virus. The present invention also comprises therapeutic compositions having sialidase activity, including protein-based compounds having sialidase catalytic domains. Compounds of the invention can be used for treating or preventing pathogen infection, and for treating and reducing allergic and inflammatory responses. The invention also provides compositions and methods for enhancing transduction of target cells by recombinant viruses. Such compositions and methods can be used in gene therapy.Type: ApplicationFiled: May 12, 2014Publication date: November 6, 2014Applicant: Ansun Biopharma, Inc.Inventors: Fang Fang, Michael Malakhov
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Patent number: 8877466Abstract: The present invention is concerned with bacteria for the production of succinic acid. Specifically, the invention relates to a bacterial cell of the genus Pasteurella comprising a heterologous polypeptide having isocitrate lyase activity and a heterologous polypeptide having malate synthase activity. Further, the present invention contemplates a polynucleotide comprising a nucleic acid encoding a polypeptide having isocitrate lyase activity and a nucleic acid encoding a polypeptide having malate synthase activity. Finally, the present invention relates to the use of a bacterial cell of the invention for the manufacture of succinic acid.Type: GrantFiled: December 22, 2009Date of Patent: November 4, 2014Assignee: BASF SEInventors: Edzard Scholten, Stefan Haefner, Hartwig Schröder
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Patent number: 8877476Abstract: A soluble and stable form of 5-lipoxygenase (5-LOX) has been made, 5-Lox is the enzyme which initiates leukotriene biosynthesis by catalyzing the two-step transformation of arachidomc acid to leukotriene A4 (LTA4). The soluble and stable 5-LOX is suitable for a number of applications, including, but not limited to, high throughput screening of 5-LOX inhibitors, structural analysis of the enzyme's active site, designing inhibitors based on the three-dimensional structure of the enzyme's active site, and synthesis of LTA4. Using Stable-5-LOX, the crystal structure for 5-LOX has been resolved and the amino acids defining the active site determined.Type: GrantFiled: May 31, 2011Date of Patent: November 4, 2014Assignee: Board of Supervisors of Louisiana State University and Agricultural and Mechanical CollegeInventors: Marcia E. Newcomer, Sue G. Bartlett, Nathaniel C. Gilbert
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Patent number: 8878006Abstract: The present invention relates generally to the field of molecular biology and concerns a method for enhancing plant yield-related traits relative to control plants. More specifically, the present invention concerns a method for enhancing yield related traits in plants relative to control plants, by modulating, preferably increasing, expression in the roots of a plant, of a nucleic acid sequence encoding a 2-cysteine peroxiredoxin (2-Cys PRX); or by modulating expression of a nucleic acid encoding an ANN polypeptide in a plant. The present invention also concerns plants having modulated, preferably increased, expression in the roots, of a nucleic acid sequence encoding a 2-Cys PRX, or having modulated expression of a nucleic acid encoding an ANN polypeptide, which plants have enhanced yield-related traits relative to control plants. The invention also provides constructs useful in the methods of the invention.Type: GrantFiled: May 23, 2008Date of Patent: November 4, 2014Assignees: Crop Design N.V., Crop Functional Genomics CenterInventors: Sang Yeol Lee, Yang Do Choi, Ho Hee Jang, Ohkmae K. Park, Huh Sun Mi
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Patent number: 8877474Abstract: The present invention provides various GH61 protein variants comprising various amino acid substitutions. The GH61 protein variants have an improved ability to synergize with cellulase enzymes, thereby increasing the yield of fermentable sugars obtained by saccharification of biomass. In some embodiments, sugars obtained from saccharification are fermented to produce numerous end-products, including but not limited to alcohol.Type: GrantFiled: August 22, 2012Date of Patent: November 4, 2014Assignee: Codexis, Inc.Inventors: Jie Yang, Xiyun Zhang, Jungjoo Yoon, Kripa Rao, John H. Grate, David Elgart, Dipnath Baidyaroy
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Patent number: 8877502Abstract: The present invention relates to plasmid curing, and particularly to efficient and stress-free methods for displacing resident or endogenous plasmids from a host cell, such as a bacterium. The invention extends to method of displacing a plasmid comprising a post-segregational killing system from a host cell, the method comprising introducing a recombinant nucleic acid molecule into a host cell harboring a plasmid comprising a post-segregational killing (PSK) system, characterized in that the recombinant nucleic acid molecule is adapted to neutralize the toxic effects of the plasmid's post-segregational killing system, and wherein the nucleic acid molecule is also adapted to outcompete or inhibit replication of the plasmid. The invention further extends to recombinant nucleic acid molecules that can be used in this method, as well as further uses of the methods and nucleic acid molecules of the invention.Type: GrantFiled: May 9, 2007Date of Patent: November 4, 2014Assignee: The University of BirminghamInventor: Christopher Morton Thomas