Encodes An Enzyme Patents (Class 536/23.2)
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Patent number: 8877479Abstract: Compositions and methods relating to an alpha-amylase enzyme obtained from Halomonas variabilis WDG195 are described.Type: GrantFiled: February 25, 2010Date of Patent: November 4, 2014Assignee: Danisco US Inc.Inventors: Melodie Estabrook, Brian E. Jones, Marc Kolkman, Chris Leeflang, Leo P. M. Van Marrewijk
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Patent number: 8877912Abstract: The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence the mature Fa_RF7182 enzyme having an amino acid sequence of SEQ ID NO: 18. The serine protease is obtainable from Fusarium acuminatum, more preferably from the deposited strain CBS 124084. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2530 comprising the nucleotide sequence SEQ ID NO:12 deposited in Escherichia coli RF7803 under accession number DSM 22208 and plasmid pALK2531 comprising the full-length gene SEQ ID NO: 13 deposited in E. coli RF7879 under accession number DSM 22209, as well as fungal hosts, such as Trichoderm. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.Type: GrantFiled: October 30, 2013Date of Patent: November 4, 2014Assignee: AB Enzymes OyInventors: Kari Juntunen, Leena Valtakari, Susanna Makinen, Jarno Kallio, Jari Vehmaanpera, Pentti Ojapalo, Marja Paloheimo, George Szakacs
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Patent number: 8877482Abstract: The present invention relates to microbial variants producing homo-succinic acid at high yields and a method for producing homo-succinic acid using the same, more particularly, to a microbial variant constructed by disrupting a lactate dehydro-genase-encoding gene (idhA) and an acetate kinase-encoding gene (ackA), as well as a method for producing homo-succinic acid at high concentration, which comprises culturing such variants using glucose as a carbon source in anaerobic conditions.Type: GrantFiled: January 15, 2008Date of Patent: November 4, 2014Assignee: Korea Advanced Institute of Science and TechnologyInventors: Sang Yup Lee, Ji Mahn Kim, Jeong Wook Lee, Hyohak Song, Sol Choi
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Patent number: 8877475Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine.Type: GrantFiled: September 11, 2012Date of Patent: November 4, 2014Assignee: Codexis, Inc.Inventors: Christopher Savile, John M. Gruber, Emily Mundorff, Gjalt Huisman, Steven James Collier
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Patent number: 8877463Abstract: The present invention relates to Rhodanobacter ginsenosidimutans KCTC22231T-derived ginsenoside glycosidase and use thereof. The polypeptide has an activity of converting PPD (protopanaxadiol)-type saponins into in vivo absorbable and highly active deglycosylated saponins, by selective hydrolysis of a bond at a particular position of ginsenoside. The present invention also relates an amino acid sequence constituting the polypeptide, a nucleic acid sequence encoding the protein, a recombinant vector comprising the nucleic acid sequence, and a transformant transformed with the vector. The invention further provides a method for preparing Rhodanobacter ginsenosidimutans KCTC22231T-derived ginsenoside glycosidase by culturing the transformant, a method for converting PPD (protopanaxadiol)-type major ginsenoside into a rare ginsenoside that is absorbable in vivo using the protein, and a composition for converting PPD-type saponins into in vivo absorbable saponins, having the protein as an active ingredient.Type: GrantFiled: September 20, 2010Date of Patent: November 4, 2014Assignee: Korea Research Institute of Bioscience and BiotechnologyInventors: Dong-Shan An, Song-Gun Kim, Sung-Taik Lee, Wan-Taek Im, Chang-Hao Cui
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Publication number: 20140322762Abstract: The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used e.g. for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology is described, as well as uses of the enzymes and enzyme preparations.Type: ApplicationFiled: October 3, 2013Publication date: October 30, 2014Applicant: ROAL OYInventors: Jari VEHMAANPERÄ, Marika ALAPURANEN, Terhi PURANEN, Matti SIIKA-AHO, Jarno KALLIO, Satu HOOMAN, Sanni VOUTILAINEN, Teemu HALONEN, Liisa VIIKARI
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Publication number: 20140322764Abstract: What is aimed at is provision of an inexpensive and efficient saccharification method for lignocellulose using a thermostable xylanase and provision of a mutant xylanase that has a substitute amino acid residue, and that exhibits stable activity even under severe conditions in which enzymes easily inactivate, and that provides an initial rate of reaction not significantly reduced as compared to a wild-type xylanase corresponding to the mutant xylanase. Provided is a method of producing a saccharified product of lignocellulose, including contacting a lignocellulosic raw material with a thermostable xylanase, and a mutant xylanase that provides an initial rate of reaction that is at least 70% of that provided by a wild-type xylanase corresponding thereto, that has a xylanase activity after heat treatment at 50° C. for 24 hours that is at least 50% of its xylanase activity before the heat treatment, and that has a substitute amino acid residue.Type: ApplicationFiled: November 22, 2012Publication date: October 30, 2014Inventors: Hisaaki Yanai, Hiroki Tamai, Masami Osabe, Fumikazu Yokoyama, Kaoru Okakura, Atsushi Inoue
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Publication number: 20140325697Abstract: The present invention relates to the field of plant transformation with genes conferring tolerance to glyphosate. The invention particularly relates to a maize (corn) plant transformed with a gene encoding an EPSPS providing the plant tolerance to an application of glyphosate under conditions where this herbicide is effective in killing weeds. The invention particularly concerns an elite transformation event VCO-Ø1981-5 comprising the gene construct and means, kits and methods for detecting the presence of the said elite event.Type: ApplicationFiled: July 26, 2012Publication date: October 30, 2014Applicant: GenectiveInventors: Lori Artim Artim Mann, Vadim Beilinson, Nadine Carozzi, Rebekah Deter, Brian Vande Berg, Alain Toppan, Laurent Beuf, Georges Freyssinet
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Publication number: 20140322789Abstract: The present invention relates to combinations of a linker bridged gene or domain fusion reverse transcriptase enzyme, and more particularly, combinations of a linker bridged gene or domain fusion reverse transcriptase enzyme and their fusion construction utilizing for more efficient and quality DNA synthesis in reverse transcription. The composition of the invention includes a polymerase domain; a linker, consisting of 3-40 amino acids; and an RNase H domain, wherein the RNase H domain is either unmodified or modified with point mutations. The composition may further include another mutated RNase H, a mutated RNase A, and an additional linker which consists of 3-40 amino acids.Type: ApplicationFiled: April 25, 2013Publication date: October 30, 2014Inventor: Jun Euihum Lee
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Publication number: 20140325700Abstract: The present invention relates to the field of applied and environmental microorganism and agriculture. Disclosed are a thifensulfuron hydrolase gene tsmE and uses thereof. The thifensulfuron hydrolase gene tsmE has a nucleotide sequence of SEQ ID NO.1, full length of 1194 bp, and G+C content of 51.09%, and encodes 398 amino acids with an amino acid sequence of SEQ ID NO.2. The thifensulfuron hydrolase TsmE provided by the present invention can degrade completely 100 mg/L thifensulfuron within 1 hour into the herbicidally inactive product thiophenesulfonic acid; in addition, the TsmE also degrade completely 100 mg/L haloxyfop-R-methyl within 1 hour. Therefore, the thifensulfuron hydrolase gene tsmE is useful in construction of thifensulfuron-resistant transgenic crops and bioremediation of thifensulfuron or haloxyfop-R-methyl-contaminated environments.Type: ApplicationFiled: July 17, 2012Publication date: October 30, 2014Applicant: NANJING AGRICULTURAL UNIVERSITYInventors: Shunpeng Li, Jian He, Baojian Hang
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Publication number: 20140322753Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: June 30, 2014Publication date: October 30, 2014Inventors: Paul Harris, Elena Vlasenko, Marcus Sakari Kauppinnen, Elizabeth Zaretsky, Sarah Teter, Kimberly Brown
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Publication number: 20140322792Abstract: Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.Type: ApplicationFiled: May 10, 2014Publication date: October 30, 2014Applicant: The California Institute of TechnologyInventors: Edgardo Farinas, Frances H. Arnold, Ulrich Schwaneberg, Anton Glieder
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Publication number: 20140322775Abstract: It is intended to provide a novel NAD+-independent myo-inositol 2-dehydrogenase which converts myo-inositol into scyllo-inosose in the absence of NAD+; a novel enzyme scyllo-inositol dehydrogenase which stereospecifically reduces scyllo-inosose into scyllo-inositol in the presence of NADH or NADPH; and a novel microorganism which belongs to the genus Acetobacter or Burkholderia and can convert myo-inositol into scyllo-inositol. By using these enzymes or the microorganism, scyllo-inositol is produced. Furthermore, scyllo-inositol is purified by adding boric acid and a metal salt to a liquid mixture containing scyllo-inositol and a neutral saccharide other than scyllo-inositol to form a scyllo-inositol/boric acid complex, separating the complex from the liquid mixture, dissolving the thus separated complex in an acid to give an acidic solution or an acidic suspension and then purifying scyllo-inositol from the acidic solution or the acidic suspension.Type: ApplicationFiled: March 25, 2014Publication date: October 30, 2014Applicant: HOKKO CHEMICAL INDUSTRY CO., LTD.Inventors: Masanori Yamaguchi, Yuichi Kita, Tetsuya Mori, Kenji Kanbe, Akihiro Tomoda, Atsushi Takahashi, Wakako Ichikawa
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Publication number: 20140322770Abstract: The present invention relates to isolated polypeptides having peroxygenaseactivity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 28, 2012Publication date: October 30, 2014Inventors: Sara Landvik, Lars Henrik Oestergaard, Lisbeth Kalum
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Publication number: 20140322765Abstract: Nucleic acid sequences encoding chimeric polypeptides that exhibit enhanced cellulase activities are disclosed herein. These nucleic acids may be expressed in hosts such as fungi, which in turn may be cultured to produce chimeric polypeptides. Also disclosed are chimeric polypeptides and their use in the degradation of cellulosic materials.Type: ApplicationFiled: December 17, 2012Publication date: October 30, 2014Inventors: William S. Adney, Gregg T. Beckham, Eric Jarvis, Michael E. Himmel, Stephen R. Decker, Jeffrey G. Linger, Kara Podkaminer, John O. Baker, Larry Taylor, II, Qi Xu, Arjun Singh
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Publication number: 20140322751Abstract: Method for incorporating a lysine derivative (particularly an N?-benzyloxycarbonyl-lysine (Z-Lys) derivative) having useful functional group such as heavy atom, selenium, reactive functional group, fluorescent group or crosslinker, which is suitable as a non-natural amino acid, into a desired protein in a site-specific manner. A mutant pyrrolysyl-tRNA synthetase has substitution of at least one amino acid residue selected from tyrosine residue at position 306, leucine residue at position 309 and cysteine residue at position 348 each constituting a pyrrolysine-binding site in the amino acid sequence for pyrrolysyl-tRNA synthetase of SEQ ID NO:2. The substitution of the amino acid residue is: of tyrosine residue at position 306 by glycine or alanine residue, of leucine residue at position 309 by glycine or alanine residue, and/or of a cysteine residue at position 348 by valine, serine or alanine residue.Type: ApplicationFiled: April 8, 2014Publication date: October 30, 2014Applicant: RikenInventors: Shigeyuki YOKOYAMA, Kensaku SAKAMOTO, Tatsuo YANAGISAWA, Takatsugu KOBAYASHI
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Publication number: 20140322769Abstract: The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.Type: ApplicationFiled: November 14, 2012Publication date: October 30, 2014Inventors: Fabien Louis Cabirol, Haibin Chen, Anupam Gohel, Steven J. Collier, Derek J. Smith, Birgit Kosjek, Jacob Janey
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Publication number: 20140322793Abstract: Provided are various novel DNA polymerases. Provided is a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 8 by inserting nine amino acids “-A737-A738-A739-A740-A741-A742-A743-A744-A745-” between the amino acid residue at position 736 and the amino acid residue at position 737, wherein: A737 is an amino acid residue having a non-polar aliphatic side chain; A738 is an amino acid residue having a non-polar aliphatic side chain; A739 is an amino acid residue having a positively charged side chain; A740 is an amino acid residue having a positively charged side chain; A741 is an amino acid residue having a non-polar aliphatic side chain; A742 is an amino acid residue having a non-polar aliphatic side chain; A743 is any given amino acid residue; A744 is an amino acid residue having a positively charged side chain; and A745 is an amino acid residue having a non-polar aliphatic side chain).Type: ApplicationFiled: July 12, 2012Publication date: October 30, 2014Applicants: TAKARA BIO INC., KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATIONInventors: Yoshizumi Ishino, Takeshi Yamagami, Hiroaki Matsukawa, Takashi Uemori, Takehiro Sagara
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Publication number: 20140322796Abstract: The invention relates to recombinant expression of variant forms of C1 CBH1a and homologs thereof, having improved thermostability, low-pH tolerance, specific activity and other desirable properties. Also provided are methods for producing ethanol and other valuable organic compounds by combining cellobiohydrolase variants with cellulosic materials.Type: ApplicationFiled: May 9, 2014Publication date: October 30, 2014Applicant: Codexis, Inc.Inventors: Vesna Mitchell, Grzegorz Wojciechowski, Oscar Alvizo
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Publication number: 20140322768Abstract: The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 28, 2012Publication date: October 30, 2014Inventors: Sara Landvik, Lars Henrik Oestergaard, Lisbeth Kalum
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Publication number: 20140322192Abstract: The present disclosure relates to the preparation and deletion mutants of chondroitinase proteins and their use in methods for promoting the diffusion of therapeutic composition into tissues and their use for neurological functional recovery after central nervous system (“CNS”) injury or disease.Type: ApplicationFiled: February 24, 2014Publication date: October 30, 2014Applicant: ACORDA THERAPEUTICS, INC.Inventors: Elliott A. GRUSKIN, Rohini D'SOUZA, Gargi ROY, Anthony G. CAGGIANO
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Publication number: 20140325692Abstract: The present invention provides methods for light-dependent gene regulation using a light-responsive DNA-binding protein. Also provided are related nucleic acid molecules, and protein molecules, such as those encoding or comprising the light-responsive DNA-binding protein or DNA-binding sites recognizing the light-responsive DNA-binding protein. Kits using the present light-dependent gene regulation system are further provided by the present invention.Type: ApplicationFiled: November 16, 2012Publication date: October 30, 2014Inventors: Kevin H. Gardner, Laura B. Motta-Mena, Brian D. Zotowski
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Publication number: 20140322795Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.Type: ApplicationFiled: July 14, 2014Publication date: October 30, 2014Inventors: Carsten Andersen, Christel Thea Jorgensen, Henrik Bisgaard-Frantzen, Allan Svendsen, Soren Kjaerulff
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Patent number: 8871999Abstract: The present invention relates to genes, proteins and methods comprising molecules that alter amino acid levels. In one embodiment, the present invention relates to altering guanidino substrate hydrolysis activities in plants, arthropods and microorganisms using molecules within the arginase family and other molecules that alter an amino acid levels. In ones embodiment, the present invention relates to altering threonine substrate deamination and dehydration activities in plants, arthropods and microorganisms using molecules within the threonine deaminase family and other molecules that alter amino acid levels. In one embodiment, the present invention relates to using genes, proteins and methods comprising arginase or threonine deaminase for altering the pathophysiology of plants, arthropods and microorganisms. In a preferred embodiment, the present invention relates to altering guanidino substrate hydrolysis activity in plants, arthropods, and microorganisms using arginase.Type: GrantFiled: October 31, 2005Date of Patent: October 28, 2014Assignee: Board of Trustees of Michigan State UniversityInventors: Gregg A. Howe, Hui Chen
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Patent number: 8871485Abstract: Disclosed herein are modified carbonic anhydrase enzymes, and a process of using same for the extraction, production and purification of carbon dioxide gas. More particularly, modified carbonic anhydrase enzymes are used for the production, purification of carbon dioxide and the products of the hydration reaction, hydrogen and bicarbonate ions Also, this technology is used to enhance the production of carbon dioxide in blood or in reverse osmosis desalination to remove carbon dioxide. Specifically, the invention relates to a modified carbonic anhydrase enzyme possessing improved activity and a process whereby immobilized modified carbonic anhydrase contained within a reactor device catalyzes the reversible hydration of carbon dioxide.Type: GrantFiled: June 22, 2011Date of Patent: October 28, 2014Assignee: University of Florida Research Foundation, Inc.Inventors: Robert McKenna, David N. Silverman
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Patent number: 8871479Abstract: The invention relates to applications of the cellulase Cel5H of Saccharophagus degradans and its homologues, functional fragments and/or variants and engineered forms thereof, in the context of recombinant, more particularly solventogenic microorganisms, more particularly C. acetobutylicum. The invention also characterizes a novel domain of the Cel5H cellulase with a putative cellulose-binding module function, and its uses in chimeric proteins for depolymerization of cellulose containing substrates.Type: GrantFiled: November 26, 2009Date of Patent: October 28, 2014Assignees: Total Marketing Services, Le Centre National de Recherche Scientifiques (CNRS), L'Institut National des Sciences Appliquees (INSA), Universite d'Aix-MarseilleInventors: Henri-Pierre Fierobe, Angélique Chanal-Vial, Anne-Laure Molinier, Chantal Tardif, Luc Dedieu
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Patent number: 8871489Abstract: This invention relates to the metabolic evolution of a microbial organism previously optimized for producing an organic acid in commercially significant quantities under fermentative conditions using a hexose sugar as sole source of carbon in a minimal mineral medium. As a result of this metabolic evolution, the microbial organism acquires the ability to use pentose sugars derived from cellulosic materials for its growth while retaining the original growth kinetics, the rate of organic acid production and the ability to use hexose sugars as a source of carbon. This invention also discloses the genetic change in the microorganism that confers the ability to use both the hexose and pentose sugars simultaneously in the production of commercially significant quantities of organic acids.Type: GrantFiled: November 17, 2010Date of Patent: October 28, 2014Assignee: Myriant CorporationInventors: Tammy Grabar, Wei Gong, R. Rogers Yocum
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Patent number: 8871516Abstract: The present invention is directed to a method for preparing an expression vector encoding a tailored recombinase, wherein said tailored recombinase recombines asymmetric target sites within the LTR of proviral DNA of a retrovirus inserted into the genome of a host cell and is useful as means for excising the provirus from the genome of the host cell. The present invention further relates to an in vitro-method of optimising the treatment of a retroviral infection of a subject and to the use of tailored recombinases for the preparation of pharmaceutical compositions for reducing the viral load in a subjected infected by a retrovirus.Type: GrantFiled: January 3, 2008Date of Patent: October 28, 2014Assignees: Technische Universität Dresden, Max-Planck-Gesellschaft zur Förderung der Wissenschaften E.V., Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität HamburgInventors: Joachim Hauber, Frank Buchholz, Ilona Hauber, Francis A Stewart, Indrani Sarkar
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Patent number: 8871445Abstract: The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.Type: GrantFiled: April 23, 2014Date of Patent: October 28, 2014Assignees: The Broad Institute Inc., Massachusetts Institute of Technology, President and Fellows of Harvard CollegeInventors: Le Cong, Feng Zhang
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Patent number: 8871488Abstract: The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.Type: GrantFiled: June 15, 2011Date of Patent: October 28, 2014Assignee: Butamax Advanced Biofuels LLCInventors: Michael Dauner, Lori Ann Maggio-Hall, Jean-Francois Tomb
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Publication number: 20140314727Abstract: The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desatorase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids.Type: ApplicationFiled: July 3, 2014Publication date: October 23, 2014Applicant: Commonwealth Scientific and Industrial Research OrganisationInventors: Surinder Pal Singh, Stanley Suresh Robert, Peter David Nichols, Susan Irene Ellis Blackburn, Xue-Rong Zhou, James Robertson Petrie, Allan Graham Green
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Publication number: 20140315246Abstract: The present invention provides a novel endo-?-N-acetylglucosaminidase (Endo-Om) using a transformant produced by cloning an endo-?-N-acetylglucosaminidase (Endo-Om) gene originated from a methylotrophic yeast Ogataea minuta IFO10746 strain. The Endo-Om according to the present invention has a specific activity 13-fold higher than that of known Endo-M and a Vmax value 55-fold higher than that of the known Endo-M, and is useful for the analysis of the structures of sugar chains, including complex type sugar chains, in glycoproteins and the modification of the sugar chains.Type: ApplicationFiled: October 3, 2012Publication date: October 23, 2014Applicant: National Institute of Advanced Industrial Science and TechnologyInventors: Yasunori Chiba, Satoshi Murakami, Hisashi Narimatsu
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Publication number: 20140315775Abstract: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved stability in the presence of a metal chelator. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications comprising citrate. In some particularly preferred embodiments, the present invention provides methods and compositions comprising variant neutral metalloprotease(s) engineered to resist citrate-induced autolysis.Type: ApplicationFiled: February 24, 2014Publication date: October 23, 2014Applicant: Danisco US Inc.Inventors: RONALDUS W.J. HOMMES, AMY D. LIU, ANDREW SHAW, LOUISE WALLACE
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Publication number: 20140314843Abstract: The present invention provides engineered phenylalanine ammonia-lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia-lyase (PAL) polypeptides.Type: ApplicationFiled: April 17, 2014Publication date: October 23, 2014Applicant: Codexis, Inc.Inventors: Gjalt W. Huisman, Nicholas J. Agard, Benjamin Mijts, Jonathan Vroom, Xiyun Zhang
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Publication number: 20140317778Abstract: The invention further relates to vectors, host cells, seeds, and plants comprising such a nucleic acid molecule. One aspect of the invention is an isolated antibody or antigen binding fragment thereof that specifically binds to a polypeptide molecule of the present invention. One aspect of the invention is a plane or plant cell transfected by a vector of the present invention. One aspect of the invention relates to isolated nucleic acid molecules and fragments thereof encoding enzymes or proteins involved in disease resistance in jute.Type: ApplicationFiled: June 8, 2012Publication date: October 23, 2014Applicant: BANGLADESH JUTE RESEARCH INSTITUTEInventors: Maqsudul Alam, Haseena Khan, Mahboob Zaman, Mohammed K. Uddin, Mohammed S. Haque, Mohammed S. Islam, Muhammad S. Azam, Niaz Mahmood
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Publication number: 20140315273Abstract: A ?-mannanase having increased enzymaic activity is disclosed. The ?-mannanase has a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of Tyrosine at position 216 with Tryptophan.Type: ApplicationFiled: September 5, 2013Publication date: October 23, 2014Applicant: Dongguan APAC Biotechnology CO., Ltd.Inventors: Rey-Ting Guo, Jian-Wen Huang, Ya-Shan Cheng, Tzu-Hui Wu, Hui-Lin Lai, Cheng-Yen Lin, Ting-Yung Huang
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Publication number: 20140315272Abstract: The present invention provides a novel nucleic acid sequence, designated LIP2, encoding a lipolytic enzyme and the corresponding encoded amino acid sequences. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding at least one novel lipolytic enzyme, recombinant lipolytic enzyme proteins and methods for producing the same.Type: ApplicationFiled: April 21, 2014Publication date: October 23, 2014Applicant: DANISCO US INC.Inventors: BRIAN E. JONES, WILLIAM D. GRANT, SHAUN HEAPHY, SUSAN GRANT, HELEN REES
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Publication number: 20140317786Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 16, 2012Publication date: October 23, 2014Inventor: Marc Morant
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Patent number: 8865637Abstract: The present invention relates to variant lysozymes. The present invention also relates to polynucleotides encoding the variant lysozymes and to nucleic acid constructs, vectors, and host cells comprising the polynucleotide.Type: GrantFiled: February 25, 2011Date of Patent: October 21, 2014Assignee: Novozymes AlsInventor: Leonardo De Maria
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Patent number: 8865451Abstract: The present disclosure relates to transgenic algae having increased growth characteristics, and methods of increasing growth characteristics of algae. In particular, the disclosure relates to transgenic algae comprising a glutamine phenylpyruvate transaminase transgene and to transgenic algae comprising a glutamine phenylpyruvate transaminase transgene and a glutamine synthetase.Type: GrantFiled: February 28, 2011Date of Patent: October 21, 2014Assignees: Los Alamos National Security, LLC, University of Maine System Board of TrusteesInventors: Pat J. Unkefer, Penelope S. Anderson, Thomas J. Knight
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Patent number: 8865439Abstract: The invention provides a non-naturally occurring microbial organism having a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The invention additionally provides a method for producing 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid. The method can include culturing a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid producing microbial organism expressing at least one exogenous nucleic acid encoding a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway enzyme in a sufficient amount and culturing under conditions and for a sufficient period of time to produce 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid.Type: GrantFiled: July 10, 2012Date of Patent: October 21, 2014Assignee: Genomatica, Inc.Inventors: Anthony P. Burgard, Mark J. Burk, Robin E. Osterhout, Priti Pharkya
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Patent number: 8865446Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: November 14, 2013Date of Patent: October 21, 2014Assignees: Novozymes A/S, Novozymes, Inc.Inventors: Lan Tang, Ye Liu, Junxin Duan, Wenping Wu, Randall Kramer
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Patent number: 8865447Abstract: The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: December 20, 2013Date of Patent: October 21, 2014Assignee: Novozymes Inc.Inventor: Nikolaj Spodsberg
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Patent number: 8865406Abstract: The invention provides for engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are compositions and methods related to components of a CRISPR complex particularly comprising a Cas ortholog enzyme.Type: GrantFiled: March 24, 2014Date of Patent: October 21, 2014Assignees: The Broad Institute Inc., Massachusetts Institute of TechnologyInventors: Feng Zhang, Fei Ran
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Publication number: 20140308731Abstract: Two novel cytochrome P450 genes are isolated from sorghum, each gene encoding a protein having pentadecatrienyl resorcinol hydroxylase activity. Expression vectors containing these sequences are made and used to elevate levels of pentadecatrienyl resorcinol hydroxylase in transgenic cells and organisms.Type: ApplicationFiled: April 15, 2013Publication date: October 16, 2014Inventor: The United States of America, as Represented by the Secretary of Agriculture
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Publication number: 20140305809Abstract: The present invention relates to mutants of the Penicillium amagasakiense glucose oxidase (GOx) enzyme which are of use for assaying glucose and to the development in particular of glucose electrodes and of biocells which use glucose as fuel.Type: ApplicationFiled: September 7, 2012Publication date: October 16, 2014Applicant: CENTRE NATIONAL DE LA RECHERCHE SCIENTIQUEInventors: Nicolas Mano, Olivier Courjean, Emilie Tremey, Sebastien Gounel
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Publication number: 20140308267Abstract: The present invention relates to the pharmaceutical field. Specifically, it contemplates a polynucleotide encoding a neurotoxin polypeptide exhibiting a reduced duration of the biological effect in a subject, wherein said polypeptide comprises at least one E3 ligase recognition motif in the light chain, wherein said E3 ligase recognition motif is preferably a binding motif for the E3 ligase MDM2. The invention further pertains to polypeptides encoded by the polynucleotide of the invention as well as polypeptides comprising one or more amino acid substitutions. Further encompassed by the present invention are vectors and host cells comprising the said polynucleotide, polypeptides encoded thereby and antibodies specifically binding to the polypeptides. Moreover, the invention relates to medicaments comprising said polynucleotides and polypeptides, as well as specific therapeutic applications thereof. Furthermore, the present invention contemplates methods for the manufacture of the polypeptides and medicaments.Type: ApplicationFiled: November 8, 2012Publication date: October 16, 2014Inventors: Michael Schmidt, Jurge Frevert, Fred Hofmann, Gerhard Groer
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Publication number: 20140308291Abstract: The present disclosure provides immunogenic compositions useful in prevention and treatment of Staphylococcus aureus infection. In particular, the disclosure provides methods of inducing an immune response against a panton-valentine leukocidin (PVL)-expressing S. aureus, methods of preventing or treating S. aureus infections, and composition for preventing or treating S. aureus infections.Type: ApplicationFiled: November 30, 2012Publication date: October 16, 2014Inventors: Mohammad Javad Aman, Rajan Prasad Adhikari, Hatice Karauzum, Jawad Sarwar, Sergey Shulenin, Sathya Venkataramani, Kelly Lyn Warfield, Tam Luong Nguyen
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Publication number: 20140309141Abstract: Methods and compositions for detection of the modulators of proteolytic enzymes, particularly cysteine proteases, are disclosed.Type: ApplicationFiled: September 21, 2012Publication date: October 16, 2014Inventors: Craig Leach, James Strickler, Michael Eddins
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Publication number: 20140308736Abstract: The present invention provides engineered protease variants. In particular, the protease variants comprise combinable mutations at selected surface positions that affect the charge and/or hydrophobicity of the enzyme to enhance at least one desired property of the resulting variant enzyme in a chosen application. Compositions comprising the protease variants, and methods for using the same are also provided.Type: ApplicationFiled: May 2, 2014Publication date: October 16, 2014Applicant: DANISCO US INC.Inventors: Luis G. Cascao-Pereira, David A. Estell, James T. Kellis, JR.