Encodes An Enzyme Patents (Class 536/23.2)
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Patent number: 8921090Abstract: The invention relates to acyl-CoA-independent methods of producing a wax ester in recombinant host cells engineered to express an acyl-ACP wax ester synthase, and an alcohol-forming acyl-ACP reductase. The methods of the invention may take place in photosynthetic microorganisms, and particularly in cyanobacteria. Isolated nucleotide molecules and vectors expressing an acyl-ACP wax ester synthase and/or an alcohol-forming acyl-ACP reductase, recombinant host cells expressing an acyl-ACP wax ester synthase and optionally an alcohol-forming acyl-ACP reductase, and systems for producing a wax ester via an acyl-CoA-independent pathway, are also provided.Type: GrantFiled: March 6, 2012Date of Patent: December 30, 2014Assignee: ExxonMobil Research and Engineering CompanyInventors: Erik Holtzapple, John H. Verruto
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Patent number: 8921080Abstract: The invention relates to an isolated polypeptide having esterase activity comprising an amino acid sequence shown in any one of SEQ ID NO's 2, 4, 6, 8, 10, 12 or 14 or a homologue thereof, comprising an amino acid substitution or deletion of one or more amino acids as shown in said SEQ ID NO's and resulting in a mutant polypeptide having an increased concentration of the fraction of the mutant polypeptide being present as an active and soluble protein in cleared lysate of the mutant polypeptide expressed in E. coli relative to the concentration of the fraction of the polypeptide without the mutation being present as an active and soluble protein in cleared lysate of the polypeptide without the one or more deletion or substitution expressed in E. coli under the same conditions. The invention also relates to nucleic acid encoding the polypeptides according to the invention, and the use of the polypeptides.Type: GrantFiled: April 26, 2010Date of Patent: December 30, 2014Assignee: DSM IP Assets B.V.Inventors: Martin Kietzmann, Harald Pichler, Helmut Schwab, Amin El-Heliebi, Christine Winkler, Andreas Braun
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Patent number: 8921654Abstract: According to this invention, a gene cluster involved in the non-mevalonate pathway of Hevea brasiliensis was obtained and nucleotide sequences of these genes were determined. The gene cluster according to this invention involved in the IPP biosynthesis in the non-mevalonate pathway is involved in the biosynthesis of vitamin E and carotenoids. Therefore, the Hevea brasiliensis obtained by introducing the gene cluster of the present invention can be expected to produce high-quality rubber with improved permanence.Type: GrantFiled: September 2, 2010Date of Patent: December 30, 2014Assignee: Bridgestone CorporationInventors: Eiichiro Fukusaki, Tomoki Sando, Norie Watanabe, Akio Kobayashi, Teuku Tajuddin
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Patent number: 8921082Abstract: A method for producing xylitol by fermentation of lignocellulosic hydrolysates without detoxification is provided. By using the originally isolated yeast Candida sp., xylose can be effectively converted into xylitol. The invention also provides the Candida strain having high furfural tolerance, and is capable to produce xylitol from various types of non-detoxified lignocellulosic hydrolysates, in which the overall utilization of xylose in hydrolysate can reach over 95%.Type: GrantFiled: March 1, 2013Date of Patent: December 30, 2014Assignee: Institute of Nuclear Energy Research Atomic Energy Council, Executive YuanInventors: Chiung-Fang Huang, Yi-Feng Jiang, Ting-Hsiang Lin, Gia-Luen Guo, Wen-Song Hwang, Jia-Baau Wang
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Publication number: 20140377407Abstract: This invention relates to a novel alpha-amylase, a process for its preparation and the use of the amylase. The invention relates to a newly identified polynucleotide sequence from Alicyclobacillus pohliae comprising a gene that encodes the novel alpha-amylase enzyme. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional protein of the gene. The invention also relates to methods of using these proteins in industrial processes, for example in food industry, such as the baking industry. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins and cells.Type: ApplicationFiled: January 29, 2013Publication date: December 25, 2014Applicant: DSM IP ASSETS B.V.Inventor: Lucie Parenicova
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Publication number: 20140377800Abstract: The present invention relates to methods of optimization of a protein coding sequences for expression in a given host cell. The methods apply genetic algorithms to optimise single codon fitness and/or codon pair fitness sequences coding for a predetermined amino acid sequence. In the algorithm generation of new sequence variants and subsequent selection of fitter variants is reiterated until the variant coding sequences reach a minimum value for single codon fitness and/or codon pair fitness. The invention also relates to a computer comprising a processor and memory, the processor being arranged to read from and write into the memory, the memory comprising data and instructions arranged to provide the processor with the capacity to perform the genetic algorithms for optimisation of single codon fitness and/or codon pair fitness.Type: ApplicationFiled: May 20, 2014Publication date: December 25, 2014Inventors: Johannes Andries ROUBOS, Noel Nicolaas Maria Elisabeth VAN PEIJ
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Publication number: 20140377827Abstract: The present invention provides hybrid polypeptides having cellobiohydrolase activity. The present invention also provides polynucleotides encoding the hybrid polypeptides; nucleic acid constructs, vectors and host cells comprising the polynucleotides; and processes of using the hybrid polypeptides.Type: ApplicationFiled: December 21, 2012Publication date: December 25, 2014Inventors: Ye Liu, Tarana Shaghasi
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Publication number: 20140377813Abstract: The invention provides a microbial eukaryotic cell capable of utilizing C5 sugars, in particular xylose. Another objective of the invention is to provide an improved protein sequence to enable eukaryotic cells to degrade C5 sugars. The present invention thus provides protein comprising an amino acid sequence having at least 75% identity, preferably 80% identity, most preferably 90% identity, most highly preferably 95% identity to SEQ ID NO. 2 or SEQ ID NO. 8 and having xylose-isomerase activity in a eukaryotic cell.Type: ApplicationFiled: February 7, 2013Publication date: December 25, 2014Inventors: Zdravko Dragovic, Christian Gamauf, Christoph Reisinger, Ulrich Kettling
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Publication number: 20140377840Abstract: A nucleic acid sequence, including an isolated, purified or recombinant nucleic acid sequence, includes: (a) a nucleic acid sequence encoding a polypeptide for stimulating embryonic development, namely, a PLC-zeta; PLC? amino acid sequence, capable of triggering calcium oscillations in oocytes; (b) a sequence substantially homologous to or that hybridizes to sequence (a) under stringent conditions; (c) a sequence substantially homologous to or that hybridizes to the sequences (a) or (b) but for degeneracy of the genetic code; and (d) an oligonucleotide specific for any of the sequences (a), (b) or (c) above.Type: ApplicationFiled: April 14, 2014Publication date: December 25, 2014Inventor: Francis Anthony Lai
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Publication number: 20140377811Abstract: The present invention concerns micro-organisms which present cellulases on their surface. Corresponding micro-organisms were produced with the aid of corresponding plasmids which encode a section comprising a signal peptide, a heterologous cellulase, an optional protease recognition site, a transmembrane linker and a transporter domain of an autotransporter or a variant thereof. Such micro-organisms were advantageously used in the conversion of cellulose into cellobiose and/or glucose. It was also possible to recover the micro-organisms from the reaction mixture following conversion from simple substrates. Also, a combination of various micro-organisms, which were populated with exocellulases, endocellulases and beta-glucosidases, were used to produce glucose from cellulose or wood.Type: ApplicationFiled: March 11, 2014Publication date: December 25, 2014Applicant: ZYRUS Beteiligungsgesellschaft mbH & Co. Patente I KGInventors: Michael Jurgens, Andreas Rohardt, Ruth Maas, Tatjana Brossette
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Publication number: 20140378315Abstract: Methods, assays, compositions and kits for the ligation of short polynucleotides are presented herein. The short polynucleotides are optionally no more than 7 nucleotides in length, and can be as short as 3 or 4 nucleotides in length. The ligation is optionally performed by CV ligase.Type: ApplicationFiled: January 17, 2012Publication date: December 25, 2014Applicant: LIFE TECHNOLOGIES CORPORATIONInventor: Stephen Hendricks
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Publication number: 20140377868Abstract: Methods and constructs for RNA-guided targeting of transcriptional activators to specific genomic loci.Type: ApplicationFiled: March 14, 2014Publication date: December 25, 2014Inventors: J. Keith Joung, Morgan Maeder
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Publication number: 20140377248Abstract: This invention relates to a novel method for producing di-chain proteins for use in humans from single-chain precursors, including di-chain clostridial neurotoxins. The method comprises the step of expressing a nucleic acid sequence encoding a single-chain precursor comprising a thrombin-cleavage site and the step of cleaving the single-chain precursor with a human factor Xa or a human thrombin, particularly a human thrombin drug product authorized for human therapeutic use. The invention further relates to novel di-chain clostridial neurotoxins and nucleic acid sequences encoding such novel di-chain clostridial neurotoxins.Type: ApplicationFiled: December 21, 2012Publication date: December 25, 2014Inventors: Swen Grein, Kerstin Hoelscher, Annett Eylenstein
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Publication number: 20140377810Abstract: Fusion proteins comprising a single strand DNA binding protein and a nucleic acid polymerase (e.g. DNA polymerase or reverse transcriptase). These high fidelity proteins are suitable for use in nucleic acid amplification methods, including the polymerase chain reaction (PCR).Type: ApplicationFiled: August 4, 2014Publication date: December 25, 2014Inventors: Jun LEE, Robert Potter, David Mandelman
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Publication number: 20140377815Abstract: The present invention relates to a method of producing carbamoyl phosphate, the method comprising reacting ammonia, ATP, bicarbonate and CO2, or a hydrated form thereof, in a composition in the presence of a carbamate kinase, wherein the ammonia and CO2, or hydrated form thereof, are converted to carbamate in a chemical reaction and the carbamate and ATP are converted to carbamoyl phosphate in an enzyme-catalysed reaction by the carbamate kinase, and wherein the pH of the composition is about 8 to about 12. The invention also relates to methods of producing urea.Type: ApplicationFiled: June 15, 2012Publication date: December 25, 2014Inventors: James Edward Hennessy, Amy Philbrook, Daniel Miles Bartkus, Christopher John Easton, Colin Scott, John G. Oakeshott, Hye-Kyung Kim, Melissa Jane Latter
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Publication number: 20140380529Abstract: The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: September 5, 2014Publication date: December 25, 2014Inventor: Nikolaj Spodsberg
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Publication number: 20140377841Abstract: The present compositions and methods relate to an endo-?-mannanase cloned from Bacillus agaradhaerens, polynucleotides encoding the endo-?-mannanase, and methods of use thereof. Formulations containing the endo-?-mannanase are highly suitable for use as detergents.Type: ApplicationFiled: July 1, 2014Publication date: December 25, 2014Applicant: DANISCO US INC.Inventors: Brian E. Jones, Marc Kolkman, Zhen Qian, Brian Sogaard Laursen, Karsten M. Kragh, Sina Pricelius, Zheyong Yu, Lilia Maria Babe, Melodie Estabrook
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Patent number: 8916373Abstract: The present invention in the art of biochemistry claims novel and non-naturally occurring mutant human superoxide dismutase 1 (hsod1) variant polypeptides, their encoding nucleic acids, and recombinant cells thereof. The inventor rationally designed mutant hsod1 variants using structural observations and complimentary experimentation. The designed mutant hsod1 variant products claimed have multiple potential industrial applications including as novel therapeutics.Type: GrantFiled: June 1, 2011Date of Patent: December 23, 2014Inventor: Aron S Workman
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Patent number: 8916369Abstract: A maltogenic a-amylase from Trichoderma reesei (TrAA) and variants thereof are useful in the production of high-maltose syrups from liquefied starch. Particularly high maltose concentrations are achieved when TrAA is used in the presence of a pullulanase. Expression hosts and encoding nucleic acids useful for producing TrAA and its variants also are provided.Type: GrantFiled: March 12, 2008Date of Patent: December 23, 2014Assignee: Danisco US Inc.Inventors: Gang Duan, Kathy Qian, Martijn Scheffers, Jayarama K. Shetty, Pieter Van Solingen
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Patent number: 8916379Abstract: The present invention provides novel mammalian alpha-kinase proteins: melanoma alpha-kinase (MK), heart alpha-kinase (HK), kidney alpha-kinase (KK), skeletal muscle alpha-kinase (SK), and lymphocyte alpha-kinase (LK). In particular, a novel kinase type is herein provided, characterized by the presence of an alpha-kinase catalytic domain and an ion channel domain. Isolated nucleic acids of the alpha-kinases MK, HK, KK, SK and LK are provided. Methods for making the novel alpha-kinases, cells that express the alpha-kinases and methods for treating an animal in need of either increased or decreased activity of the alpha-kinases are provided.Type: GrantFiled: June 17, 2010Date of Patent: December 23, 2014Assignee: Rutgers, The State University of New JerseyInventor: Alexey G. Ryazanov
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Patent number: 8916365Abstract: Transgenic Yarrowia species are disclosed herein that comprise a polynucleotide encoding a cytosolic malic enzyme, a lipid content that is at least about 35% by weight of the dry cell weight of the Yarrowia species, and an engineered polyunsaturated fatty acid (PUFA) biosynthetic pathway, wherein overexpression of the cytosolic malic enzyme increases lipid content.Type: GrantFiled: March 14, 2013Date of Patent: December 23, 2014Assignee: E I du Pont de Nemours and CompanyInventors: Daniel Joseph Macool, Zhixiong Xue, Quinn Qun Zhu
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Patent number: 8916361Abstract: The subject invention relates to the identification of a gene involved in the elongation of polyunsaturated fatty acids containing unsaturation at the carbon 9 position (i.e., “?9-elongase”) and to uses thereof. In particular, ?9-elongase may be utilized, for example, in the conversion of linoleic acid (LA, 18:2n-6) to eicosadienoic acid (EDA, 20:2n-6). The production of dihomo-?-linolenic acid (DGLA, 20:3n-6) from eicosadienoic acid (EDA, 20:2n-6), and arachidonic acid (AA, 20:4n-6) from dihomo-?-linolenic acid (DGLA, 20:3n-6) is then catalyzed by ?8-desaturase and ?5-desaturase, respectively. AA or polyunsaturated fatty acids produced therefrom may be added to pharmaceutical compositions, nutritional compositions, animal feeds, as well as other products such as cosmetics.Type: GrantFiled: November 14, 2007Date of Patent: December 23, 2014Assignee: Abbott LaboratoriesInventors: Tapas Das, Pradip Mukerji, Padmavathy Krishnan, Amanda E. Leonard, Suzette L. Pereira
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Patent number: 8916370Abstract: The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.Type: GrantFiled: March 30, 2012Date of Patent: December 23, 2014Assignees: Danisco US Inc., The Goodyear Tire & Rubber CompanyInventors: Richard R. Bott, Marguerite A. Cervin, James T. Kellis, Jr., Joseph C. McAuliffe, Andrei Miasnikov, Caroline M. Peres, Christopher Lee Rife, Derek H. Wells, Walter Weyler, Gregory M. Whited
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Publication number: 20140370554Abstract: The present invention is to enhance the stability and enzyme activity of an L-arabitol dehydrogenase derived from Neurospora crassa using techniques from quantum mechanics and molecular mechanics and partial mutation techniques. More specifically, the present invention relates to a method for preparing an L-arabitol dehydrogenase in which a residue which affects enzyme stability is found through a screening based on quantum mechanics and molecular mechanics, and enzyme activity is enhanced by mutation of the found residue, nucleic acid molecules encoding the L-arabitol dehydrogenase, a vector including the nucleic acid molecules, a transformant including the vector, a mutant of the L-arabitol dehydrogenase and an improved L-arabitol dehydrogenase.Type: ApplicationFiled: April 18, 2013Publication date: December 18, 2014Inventors: Jung Kul Lee, Hee Jung Moon, Manish Kumar Tiwari, Tae Su Kim
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MICROORGANISMS AND METHODS FOR PRODUCTION OF 4-HYDROXYBUTYRATE, 1,4-BUTANEDIOL AND RELATED COMPOUNDS
Publication number: 20140371417Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine or related products using the microbial organisms.Type: ApplicationFiled: April 25, 2014Publication date: December 18, 2014Applicant: Genomatica, Inc.Inventors: Priti PHARKYA, Anthony P. BURGARD, Stephen J. VAN DIEN, Robin E. OSTERHOUT, Mark J. BURK, John D. TRAWICK, Michael P. KUCKINSKAS, Brian STEER -
Publication number: 20140373199Abstract: Provided are isolated polypeptides having beta-xylosidase activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 22, 2012Publication date: December 18, 2014Applicant: Novozymes Inc.Inventors: Yu Zhang, Ye Liu, Junxin Duan, Lan Tang, Brett McBrayer
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Publication number: 20140370562Abstract: The present invention provides methods and compositions for producing ?-phellandrene hydrocarbons from cyanobacteria.Type: ApplicationFiled: February 6, 2013Publication date: December 18, 2014Inventors: Anastasios Melis, Fiona K. Bentley, Hsu-Ching Chen Wintz, Andreas Zurbriggen
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Publication number: 20140370568Abstract: Specialized (iso)eugenol 4-O-methyltransferase (s-IEMT) enzymes having increased capacity for methylation of monolignols are disclosed. The s-IEMTs have unique activity favoring methylation of coniferyl alcohol versus sinapyl alcohol. Various s-IEMTs methylate ferulic acid. Means for producing the various s-IEMTs are provided. The s-IEMTs are useful for modification of lignin content and production of aromatic compounds.Type: ApplicationFiled: June 17, 2014Publication date: December 18, 2014Inventors: Chang-Jun Liu, Yuanheng Cai
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Publication number: 20140371438Abstract: The present disclosure provides compositions and methods for the prevention or treatment of ocular neovascularization, such as AMD, in a human subject, by administering subretinally a pharmaceutical composition comprising a pharmaceutically effective amount of a vector comprising a nucleic acid encoding soluble Fms-related tyrosine kinase-1 (sFlt-1) protein to the human subject.Type: ApplicationFiled: May 19, 2014Publication date: December 18, 2014Applicant: Avalanche Australia Pty Ltd.Inventors: Ian J. Constable, P. Elizabeth Rakoczy, Chooi-May Lai, Thomas W. Chalberg, JR.
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Publication number: 20140370595Abstract: The present invention provides methods of producing a product or product precursor of a biosynthetic pathway in a genetically modified host cell. The present invention also provides genetically modified host cells comprising nucleic acids encoding a scaffold polypeptide and nucleic acids comprising nucleotide sequences encoding two or more enzymes in a biosynthetic pathway. The present invention further provides nucleic acids comprising nucleotide sequences encoding scaffold polypeptides, for use in a subject method.Type: ApplicationFiled: June 25, 2014Publication date: December 18, 2014Inventors: John E. Dueber, Jay D. Keasling, Gabriel C. Wu, Ghulam Reza K. Malmirchegini
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Publication number: 20140370506Abstract: The invention provides an improved stem-loop target capture oligomer and methods of use. Such a target capture oligomer has a target-binding segment forming a loop flanked by stem segments forming a stem. The stem segments are of unequal length. Such probes show little or no binding to immobilized probes in the absence of a target nucleic acid but offer good target sensitivity. The probes are particularly useful in multiplex methods of detection in which multiple target capture oligomers are present for detecting of multiple target nucleic acids (for example, detecting multiple polymorphic forms of a target gene).Type: ApplicationFiled: February 1, 2013Publication date: December 18, 2014Applicant: GEN-PROBE INCORPORATEDInventors: James Carlson, Reinhold Pollner, Steven T. Brentano
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Publication number: 20140369986Abstract: The present invention provides methods and compositions to reduce growth of microbial colonies, including infections, and includes therapeutic compositions, methods for treatment of infections, and methods for identifying additional such compositions.Type: ApplicationFiled: May 15, 2014Publication date: December 18, 2014Applicant: GangaGen, Inc.Inventors: Sriram Padmanabhan, Vivek Daniel Paul, R.Sanjeev Saravanan, Bharathi Sriram
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Publication number: 20140370514Abstract: The present invention provides an endonuclease I or enzymatically active fragment thereof wherein said endonuclease I has the sequence of SEQ ID No. 4 or a sequence which is at least 70% identical thereto and wherein the amino acid residue which is immediately N-terminal of the FYCGC pentapeptide motif has been substituted with a residue which is negatively charged as well as nucleic acid molecules encoding these enzymes and methods of removing contaminating polynucleotides from a sample using these enzyme.Type: ApplicationFiled: February 18, 2013Publication date: December 18, 2014Applicant: BIOTEC PHARMACONB ASAInventors: Olav Lanes, Linda Havdalen, Terese Solstad, Marit Lorentzen, Bjørn Altermark, Ingar Leiros, Ronny Helland
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Publication number: 20140370552Abstract: The present invention provides endoglucanase 1b (EG1b) variants suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce EG1b variants, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.Type: ApplicationFiled: December 18, 2012Publication date: December 18, 2014Inventors: Ee Lui Ang, Ellen D. Eberhard, Xiyun Zhang, Wei Zhang, Jing Tian, Derek J. Smith, Vesna Mitchell
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Patent number: 8911983Abstract: The endogenous pnp gene encoding polynucleotide phosphorylase in the Zymomonas genome was identified as a target for modification to provide improved xylose utilizing cells for ethanol production. The cells are in addition genetically modified to have increased expression of ribose-5-phosphate isomerase (RPI) activity, as compared to cells without this genetic modification, and are not limited in xylose isomerase activity in the absence of the pnp modification.Type: GrantFiled: December 12, 2012Date of Patent: December 16, 2014Assignee: E I Du Pont de Nemours and CompanyInventors: Perry G G Caimi, Min Qi, Luan Tao, Paul V Viitanen, Jianjun Yang
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Patent number: 8911984Abstract: Disclosed is a thermostable tannase derived from a microorganism. Specifically disclosed is a thermostable tannase derived from Aspergillus awamori or Aspergillus niger. A preferred embodiment of the tannase has the following chemoenzymatic properties: (1) activity: to act on a depside bond to thereby cause hydrolysis; (2) molecular weight: about 230,000 Da (as measured by gel filtration); and (3) thermal stability: stable at a temperature up to 65° C. (pH 5.0, 30 min.).Type: GrantFiled: November 25, 2013Date of Patent: December 16, 2014Assignee: Amano Enzyme Inc.Inventors: Megumi Nakagawa, Naoki Matsumoto, Hidoshi Amano, Shotaro Yamaguchi
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Patent number: 8911982Abstract: The invention relates to cells, nucleic acids, and enzymes, the use thereof for producing sophorolipids, and methods for producing sophorolipids.Type: GrantFiled: October 19, 2010Date of Patent: December 16, 2014Assignee: Evonik Degussa GmbHInventors: Steffen Schaffer, Mirja Wessel, Anja Thiessenhusen
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Patent number: 8911981Abstract: Methods for the evolution of NADPH specific ketol-add reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.Type: GrantFiled: March 15, 2013Date of Patent: December 16, 2014Assignee: Butamax Advanced Biofuels LLCInventors: Yougen Li, Der-Ing Liao, Mark J. Nelson, Daniel P. Okeefe
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Publication number: 20140360403Abstract: A method for producing organic-inorganic composite materials includes at least one biomineralising protein and/or polypeptide-encoding recombinant polynucleotide being introduced into at least one host cell, preferably from the class of slime moulds, and the expressed protein and/or polypeptide influencing the formation of inorganic particles in an extracellular matrix of the host cell.Type: ApplicationFiled: December 19, 2012Publication date: December 11, 2014Inventors: Ingrid Weiss, Eva Weber, Magdalena Eder, Eduard Arzt, Andreas Simon Schneider
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Publication number: 20140364483Abstract: Disclosed are novel splicing variants of the genes associated with prostate cancer risk and survival, particularly splicing variants of PIK3CD, FGFR3, TSC2, RASGRP2, ITGA4, MET, NF1 and BAK1. The disclosure also relates risk assessment, detection, diagnosis, or prognosis of prostate cancer. More specifically, this disclosure relates to the detection of certain splicing variants of PIK3CD, FGFR3, TSC2, RASGRP2, ITGA4, MET, NF1 and BAK1.Type: ApplicationFiled: September 20, 2012Publication date: December 11, 2014Inventors: Norman H. Lee, Steven R. Patierno, Bi-Dar Wang
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Publication number: 20140366216Abstract: Stress tolerance in plants and plant cells is achieved by using nucleotide sequences encoding enzymes involved in the NAD salvage synthesis pathway and/or the NAD de novo synthesis pathway e.g. for overexpression in plants.Type: ApplicationFiled: August 11, 2014Publication date: December 11, 2014Inventors: Marc DE BLOCK, Michael Metzlaff, Véronique Gossele
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Publication number: 20140363844Abstract: An enzyme useful for producing cyclic peptides from linear peptide precursors and a gene encoding the enzyme are described. The enzyme is particularly useful for producing segetalins from linear presegetalin precursors. The linear presegetalin precursors may be derived from other linear presegetalin precursors farther upstream in the biosynthetic synthesis of the segetalin.Type: ApplicationFiled: December 7, 2012Publication date: December 11, 2014Inventors: Carla Jann Siobhan Barber, Darwin Wilfred Reed, Janet Anne Condie, Sheila Diniwe Sherin Chiwocha, Patrick Smithers Covello
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Publication number: 20140363875Abstract: Provided are various novel DNA polymerases. Provided are: a DNA polymerase comprising: an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 12, which has a substitution of arginine at position 651 by an amino acid residue having a negatively charged side chain, preferably by asparatic acid or glutamic acid, more preferably by glutamic acid; and a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 14, which has a substitution of proline at position 653 by an amino acid residue having a negatively charged side chain, preferably by asparatic acid or glutamic acid, more preferably by glutamic acid.Type: ApplicationFiled: July 12, 2012Publication date: December 11, 2014Applicant: KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATIONInventors: Yoshizumi Ishino, Takeshi Yamagami, Hiroaki Matsukawa
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Publication number: 20140363848Abstract: The present invention provides, among other things, chimeric DNA polymerases containing heterologous domains having sequences derived from at least two DNA polymerases that have at least one distinct functional characteristics (e.g., elongation rate, processivity, error rate or fidelity, salt tolerance or resistance) and methods of making and using the same. In some embodiments, the present invention can combine desired functional characteristics (e.g., high processivity; high elongation rate; thermostability; resistance to salt, PCR additives (e.g., PCR enhancers) and other impurities; and high fidelity) of different DNA polymerases in a chimeric polymerase.Type: ApplicationFiled: May 5, 2014Publication date: December 11, 2014Applicant: KAPA BIOSYSTEMS, INC.Inventors: Bjarne FAURHOLM, Paul MCEWAN, William BOURN, Gavin RUSH
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Patent number: 8906661Abstract: An enhanced disintegrin-like domain, and metalloprotease, with an isolated human thrombospondin type 1 motif, member 13 (ADAMTS-13) that includes substitutions at one or more positions in the isolated human ADAMTS-13.Type: GrantFiled: January 28, 2014Date of Patent: December 9, 2014Assignee: The Chemo-Sero-Therapeutic Research InstituteInventor: Kenji Soejima
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Patent number: 8906665Abstract: The present disclosure identifies methods and compositions for modifying photoautotrophic organisms, such that the organisms efficiently convert carbon dioxide and light into compounds such as esters and fatty acids. In certain embodiments, the compounds produced are secreted into the medium used to culture the organisms.Type: GrantFiled: February 12, 2013Date of Patent: December 9, 2014Assignee: Joule Unlimited Technologies, Inc.Inventors: David Arthur Berry, Noubar Boghos Afeyan, Frank Anthony Skraly, Christian Perry Ridley, Dan Eric Robertson, Regina Wilpiszeski, Martha Sholl
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Patent number: 8906667Abstract: A method of increasing cellular NADPH levels by expressing one or more genes that encode an enzyme that causes the production of NADPH. The system is combined with other enzymes that require NADPH, thus improving the overall yield of the desired product.Type: GrantFiled: August 29, 2007Date of Patent: December 9, 2014Assignee: William Marsh Rice UniversityInventors: Ka-Yiu San, George Bennett, Henry Lin, Irene Martinez, Jiangfeng Zhu
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Patent number: 8906365Abstract: The application relates to antimicrobial agents against Gram-negative bacteria, in particular to fusion proteins composed of an enzyme having the activity of degrading the cell wall of Gram-negative bacteria and a peptide stretch fused to the enzyme at the N- or C-terminus, as well as pharmaceutical compositions thereof. Moreover, it relates to nucleic acid molecules encoding such a fusion protein, vectors carrying the nucleic acid molecules and host cells transformed with either the nucleic acid molecules or the vectors. In addition, it relates to such a fusion protein for use as a medicament, in particular for the treatment or prevention of Gram-negative bacterial infections, as diagnostic means or as cosmetic substance. The application also relates to the treatment or prevention of Gram-negative bacterial contamination of foodstuff, of food processing equipment, of food processing plants, of surfaces coming into contact with foodstuff, of medical devices, of surfaces in hospitals and surgeries.Type: GrantFiled: June 28, 2010Date of Patent: December 9, 2014Assignees: Lysando AG, Katholieke Universiteit Leuven, K.U. Leuven R&DInventors: Rob Lavigne, Stefan Miller, Yves Briers, Guido Volckaert, Maarten Walmagh
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Patent number: 8906663Abstract: The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.Type: GrantFiled: November 16, 2012Date of Patent: December 9, 2014Assignee: Verenium CorporationInventors: David Weiner, Ellen Chi, Jennifer Ann Chaplin, Aileen Milan, Jay M. Short, Grace DeSantis, Mark Madden, Mark J. Burk, Dan E. Robertson
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Publication number: 20140356887Abstract: Amino acid mutation(s) can be introduced to Sclerotinia sclerotiorum- or Aspergillus niger-derived glucose dehydrogenase to obtain a glucose dehydrogenase variant with significantly enhanced productivity in E. coli. The glucose dehydrogenase of the present invention is low reactive with xylose.Type: ApplicationFiled: December 28, 2012Publication date: December 4, 2014Applicant: ULTIZYME INTERNATIONAL LTD.Inventors: Koji Sode, Kazushige Mori