Encodes An Enzyme Patents (Class 536/23.2)
-
Publication number: 20140308672Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.Type: ApplicationFiled: May 23, 2014Publication date: October 16, 2014Inventors: Keith Bauer, Fred Reichert, Thomas W. Myers
-
Publication number: 20140308266Abstract: The present invention is concerned with modified neurotoxins. Specifically, it relates to a modified biologically active neurotoxin polypeptide comprising at least one poly-Glycine domain. Also contemplated is a polynucleotide encoding the modified neurotoxin polypeptide having a poly-Glycine domain fused to the N-terminus, to the C-terminus or to both of the heavy and/or light chain of the mature neurotoxin polypeptide, vector comprising it and a host cell comprising such a polynucleotide or a vector. Moreover, envisaged are the aforementioned compounds for use as a medicament for treating various diseases.Type: ApplicationFiled: November 8, 2012Publication date: October 16, 2014Inventor: Karl-Heinz Eisele
-
Publication number: 20140308725Abstract: The present invention provides isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. The invention also provides nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains.Type: ApplicationFiled: September 7, 2012Publication date: October 16, 2014Applicant: NOVOZYMES A/SInventors: Tianqi Sun, Ming Li
-
Publication number: 20140308732Abstract: The present disclosure relates to engineered ketoreductase polypeptides and uses thereof for the preparation of ?-chloroalcohols from ?-chloroketones.Type: ApplicationFiled: June 24, 2014Publication date: October 16, 2014Inventors: Yong Koy Bong, Michael Vogel, Steven J. Collier, Vesna Mitchell, Jagadeesh Mavinahalli
-
Patent number: 8858931Abstract: This invention relates to the field of anticancer therapy, and to the identification of immunogenic peptides derived from the human telomerase reverse transcriptase (hTERT). The present invention relates to polynucleotides encoding hTERT epitopes restricted to MHC class I molecule, analogs thereof and polyepitopes containing such epitopes and/or analogs. Are also included in the present invention, vector and cell comprising such polynucleotides. The present invention also concerns composition comprising hTERT polypeptides, corresponding polynucleotides, vectors and cells, for use in the treatment and/or prevention of cancer.Type: GrantFiled: July 31, 2006Date of Patent: October 14, 2014Assignees: Institut Pasteur, INSERMInventors: Pierre Langlade-Demoyen, Fransisco Garcia Pons, Olivier Adotevi, Sylvain Cardinaud, Christine Neuveut
-
Patent number: 8859251Abstract: The invention describes novel pharmaceutical compositions for the treatment of virus infections and cancer. The pharmaceutical compositions include mutant oligoadenylate synthetases (OAS) that have either enhanced cell permeability, reduced oxidative potential, improved antiviral activity, improved enzymatic activity, or absent enzymatic activity. The pharmaceutical compositions have improved drug properties and retain or have enhanced antiviral activity relative to their native forms. The pharmaceutical compositions further include chemically modified oligoadenylate synthetases, such chemical modifications being designed to increase serum stability and reduce immunogenicity in vivo. Such chemical modifications further increase drug stability and manufacturability in vitro. Compositions composed of more than ninety novel modifications are described. Also described are antibodies to polypeptides of the invention.Type: GrantFiled: July 1, 2013Date of Patent: October 14, 2014Assignee: Kineta Two, LLCInventors: Shawn P. Iadonato, Charles L. Magness, Mark Branum, Maralee McVean, Christina Scherer
-
Patent number: 8859253Abstract: The present invention relates to variants of a parent cellobiohydrolase II. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: GrantFiled: September 23, 2013Date of Patent: October 14, 2014Assignee: Novozymes, Inc.Inventor: Mark Wogulis
-
Patent number: 8859855Abstract: Disclosed are chimeric polyunsaturated fatty acid (PUFA) olyketide synthase (PKS) proteins and chimeric PUFA PKS systems, including chimeric PUFA PKS proteins and systems derived from Schizochytrium and Thraustochytrium. Disclosed are nucleic acids and proteins encoding such chimeric PUFA PKS proteins and systems, genetically modified organisms comprising such chimeric PUFA PKS proteins and systems, and methods of making and using such chimeric PUFA PKS proteins and systems.Type: GrantFiled: October 2, 2012Date of Patent: October 14, 2014Assignee: DSM IP Assets B.V.Inventors: Craig A. Weaver, Ross Zirkle, Daniel H. Doherty, James G. Metz
-
Patent number: 8859246Abstract: The invention discloses a method for production of polyhydroxybutyrate-co-polyhydroxyvalerate (PHBV) by recombinant Escherichia coli harboring plasmid containing both phaCAB and prpE. Different percentage of hydroxyvalerate can be obtained from the recombinant E. coli when cultivated in the medium containing different concentrations of propionic acid. In this patent, we provide a method that integrated all of the genes (i.e. phaCAB, vgb and prpE) required for PHBV production into a single plasmid. The plasmids were then transformed into an E. coli host. Results showed that PHBV can be produced by this recombinant E. coli, and the ration of HV to HB in the co-polymers can be regulated by addition of different concentrations of propionic acid in the medium. The percentage of HV in the co-polymers can be adjusted from about 3% up to more than 35%.Type: GrantFiled: May 29, 2012Date of Patent: October 14, 2014Assignee: Yuan Ze UniversityInventors: Chih-Ching Chien, Po-Chi Soo, Yu-Tze Horng, Shan-Yu Chen, Hsiu-Hsiung Li, Yu-Hong Wei, Wen-Ming Chen
-
Patent number: 8859023Abstract: The formation of acrylamide during heat treatment in the production of a food product is reduced by treating the raw material with an enzyme before the heat treatment. The enzyme is capable of reacting on asparagine or glutamine (optionally substituted) as a substrate or is a laccase or a peroxidase.Type: GrantFiled: May 10, 2013Date of Patent: October 14, 2014Assignee: Novozymes A/SInventors: Gitte Budolfsen, Morten Tovborg Jensen, Hans Peter Heldt-Hansen, Mary Ann Stringer
-
Patent number: 8859254Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: December 12, 2013Date of Patent: October 14, 2014Assignees: Novozymes, Inc., Novozymes A/SInventor: Marc Dominique Morant
-
Patent number: 8859255Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.Type: GrantFiled: March 12, 2013Date of Patent: October 14, 2014Assignee: Novozymes A/SInventors: Carsten Andersen, Christel Thea Jorgensen, Henrik Bisgaard-Frantzen, Allan Svendsen, Soren Kjaerulff
-
Patent number: 8859241Abstract: Provided is a novel process for producing an L-amino acid using a microorganism belonging to the genus Escherichia. According to the present invention, a process for producing an L-amino acid comprising; culturing a microorganism in which an activity of the protein of any of the following [1]-[3] is increased compared with that of the parent strain in a medium, producing and accumulating the L-amino acid in the medium, and recovering the L-amino acid from the medium: [1] a protein comprising the amino acid sequence shown in SEQ ID NO: 2 [2] a protein consisting of an amino acid sequence in which one or more amino acids are deleted, substituted or added in the amino acid sequence shown in SEQ ID NO: 2, and having YeiG activity [3] a protein consisting of an amino acid sequence having 80% or more homology to the amino acid sequence shown in SEQ ID NO: 2, and having YeiG activity.Type: GrantFiled: January 7, 2011Date of Patent: October 14, 2014Assignee: Kyowa Hakko Bio Co., Ltd.Inventors: Mikiro Hayashi, Kazuhiko Tabata
-
Patent number: 8859244Abstract: The present invention relates to a method of microbial production of L-lysine from methanol and other substrates, and particularly improving the production of L-lysine from such substrates. The invention concerns a method for producing L-lysine in B. methanolicus, said method comprising overexpressing an aspartate kinase III (AKIII) enzyme in said B. methanolicus. In particular the method may concern introducing a nucleic acid molecule comprising a nucleotide sequence encoding an AKIII enzyme into a B. methanolicus. The invention also relates to a B. methanolicus micro-organism which overexpresses an AKIII enzyme, nucleic acid molecules which encode polypeptides having AK activity, polypeptides which have AK activity and host cells and vector systems comprising the nucleic acid molecules or vector.Type: GrantFiled: May 15, 2009Date of Patent: October 14, 2014Assignee: Sinvent ASInventors: Trygve Brautaset, Øyvind Mejdell Jakobsen, Trond Ellingsen
-
Patent number: 8859232Abstract: The present invention relates to fusion proteins comprising protein light switches and methods of photomanipulating the activity of the proteins. The invention further relates to polynucleotides and vectors encoding the fusion proteins, cells comprising the fusion proteins, and methods of using the fusion proteins to study protein function and analyze subcellular activity, as well as diagnostic and therapeutic methods.Type: GrantFiled: July 1, 2010Date of Patent: October 14, 2014Assignee: The University of North Carolina at Chapel HillInventors: Klaus Hahn, Yi Wu, Brian Kuhlman
-
Publication number: 20140302006Abstract: The present invention relates to a method for suppressing neuroendocrine disease. The therapy employs use of a non-cytotoxic protease, which is targeted to a neuroendocrine tumour cell, preferably via a somatostatin or cortistatin receptor, a GHRH receptor, a ghrelin receptor, a bombesin receptor, a urotensin receptor a melanin-concentrating hormone receptor 1; a KiSS-1 receptor or a prolactin-releasing peptide receptor. When so delivered, the protease is internalised and inhibits secretion from said tumour cell. The present invention also relates to polypeptides and nucleic acids for use in said methods.Type: ApplicationFiled: April 11, 2014Publication date: October 9, 2014Applicant: Syntaxin LimitedInventors: Steven JOHNSTONE, Philip MARKS, Keith FOSTER
-
Publication number: 20140302507Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.Type: ApplicationFiled: May 15, 2014Publication date: October 9, 2014Inventors: Fred Reichert, Keith Bauer, Thomas W. Myers
-
Publication number: 20140302561Abstract: The present invention relates in particular to proteins, coding nucleic acid sequences for same, vectors comprising said coding sequences, a method for producing said proteins, and an oligosaccharide hydrolysis method using same. In particular, the invention relates to the protein of sequence SEQ ID no. 1. The present invention can be applied to the recycling of bio-natural resources formed by organisms and microorganisms including ulvans, in particular green algae.Type: ApplicationFiled: September 14, 2012Publication date: October 9, 2014Applicants: UNIVERSITÉ PIERRE ET MARIE CURIE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE- CNRS, CENTRE D'ÉTUDE ET DE VALORISATION DES ALGUESInventors: Pi Collen, William Helbert, Yannick Lerat, Jean-François Sassi
-
Publication number: 20140302075Abstract: The present invention provides aspartyl-tRNA synthetase derived proteins (DRS polypeptides) with altered cysteine content, compositions comprising the same, and methods of using such polypeptides and compositions for treating or diagnosing a variety of conditions. The DRS polypeptides of the invention have immunomodulatory properties, and exhibit improved activity and stability.Type: ApplicationFiled: December 6, 2012Publication date: October 9, 2014Inventors: Ying Ji Buechler, Chi-Fang Wu, Ryan Andrew Adams, Kristi Helen Piehl, Jeffrey Greve, John D. Mendlein
-
Publication number: 20140302508Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.Type: ApplicationFiled: May 27, 2014Publication date: October 9, 2014Inventors: Keith Bauer, Fred Reichert, Thomas W. Myers
-
Publication number: 20140304845Abstract: Methods, biomarkers, and expression signatures are disclosed for assessing the disease progression of Alzheimer's disease (AD). In one embodiment, BioAge (biological age), NdStress (neurodegenerative stress), Alz (Alzheimer), and Inflame (inflammation) are used as biomarkers of AD progression. In another aspect, the invention comprises a gene signature for evaluating disease progression. In still another embodiment, methods for evaluating disease progression are provided. In yet another embodiment, the invention can be used to identify animal models for use in the development and evaluation of therapeutics for the treatment of AD.Type: ApplicationFiled: October 26, 2012Publication date: October 9, 2014Inventors: Andrey Loboda, Michael Nebozhyn, Alexei Podtelezhnikov, David J. Stone, Keith Tanis, William J. Ray
-
Publication number: 20140302590Abstract: The present invention provides protease compositions particularly suited for dishwashing applications.Type: ApplicationFiled: April 7, 2014Publication date: October 9, 2014Applicant: Danisco US Inc.Inventors: Pieter Augustinus, Frits Goedegebuur, Ayrookaran J. Poulose, Johannes C. Van Der Laan
-
Publication number: 20140304859Abstract: Provided are isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: December 14, 2012Publication date: October 9, 2014Applicant: Novozymes Inc.Inventors: Ye Liu, Yu Zhang, Junxin Duan, Lan Tang
-
Publication number: 20140304856Abstract: This invention is in the field of plant molecular biology. More specifically, this invention pertains to isolated nucleic acid fragments encoding PAE or PAE-Like proteins in plants and seeds and the use of such fragments to modulate expression of a gene encoding PAE or PAE-Like protein activity in a transformed host cell.Type: ApplicationFiled: June 29, 2011Publication date: October 9, 2014Applicant: E I DU PONT DE NEMOURS AND COMPANYInventors: Knut Meyer, Brian Mcgonigle, Kevin L. Stecca
-
Publication number: 20140304849Abstract: The present invention provides a DNA construct that confers tolerance to transgenic corn plant. Also provided are assays for detecting the presence of the PV-ZMGT32(nk603) corn event based on the DNA sequence of the recombinant construct inserted into the corn genome and of genomic sequences flanking the insertion site.Type: ApplicationFiled: March 6, 2014Publication date: October 9, 2014Applicant: Monsanto Technology LLCInventors: Carl Frederick Behr, Gregory R. Heck, Catherine Hironaka, Jinsong You
-
Publication number: 20140302584Abstract: Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.Type: ApplicationFiled: February 25, 2014Publication date: October 9, 2014Applicant: DANISCO US INC.Inventors: Anthony DAY, Frits GOEDEGEBUUR, Peter GUALFETTI, Colin MITCHINSON, Paulien NEEFE, Mats SANDGREN, Andrew SHAW, Jerry STAHLBERG
-
Patent number: 8852583Abstract: One aspect of the present invention relates to mutants of chondroitinase ABCI. Such chondroitinase ABCI mutants exhibit altered chondroitin lyase activity or increased resistance to inactivation from stressors including exposure to UV light or heat. Methods of using chondroitinase ABCI mutant enzymes are also provided.Type: GrantFiled: July 6, 2012Date of Patent: October 7, 2014Assignee: Acorda Therapeutics, Inc.Inventors: Anthony O. Caggiano, Jennifer Iaci, Andrea Vecchione, Elizabeth Markensohn
-
Patent number: 8853377Abstract: Compositions for modulating the expression of a protein in a target cell comprising at least one RNA molecule which comprises at least one modification conferring stability to the RNA, as well as related methods, are disclosed.Type: GrantFiled: November 29, 2011Date of Patent: October 7, 2014Assignee: Shire Human Genetic Therapies, Inc.Inventors: Braydon Charles Guild, Michael Heartlein
-
Patent number: 8852913Abstract: The present invention relates to variants of a parent beta-glucosidase, comprising a substitution at one or more positions corresponding to positions 142, 183, 266, and 703 of amino acids 1 to 842 of SEQ ID NO: 2 or corresponding to positions 142, 183, 266, and 705 of amino acids 1 to 844 of SEQ ID NO: 70, wherein the variant has beta-glucosidase activity. The present invention also relates to nucleotide sequences encoding the variant beta-glucosidases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.Type: GrantFiled: December 16, 2009Date of Patent: October 7, 2014Assignee: Novozymes, Inc.Inventors: Ana Fidantsef, Michael Lamsa, Brian Gorre-Clancy
-
Patent number: 8852891Abstract: The present invention relates to a novel N-acetylglucosamine-2-epimerase and a method for preparing CMP-N-acetylneuraminic acid, more specifically, relates to a N-acetylglucosamine-2-epimerase derived from Bacteroides fragilis NCTC 9343, and a method for preparing CMP-N-acetylneuraminic acid using said N-acetylglucosamine-2-epimerase. According to the present invention, CMP-N-acetylneuraminic acid can be produced economically in a large amount through a one-step reaction using cytidine monophosphate and N-acetyl-D-glucosamine which are inexpensive substrates.Type: GrantFiled: December 7, 2007Date of Patent: October 7, 2014Assignee: Gene Chem, Inc.Inventors: Jin Suk Woo, Jae Kyung Sohng, Byung Gee Kim, Sun Youp Kang, Dea Hee Kim, Kyoung Soon Jang, Ji Young Yang, Young Soo Jung, Won Min Seo, Tae Geon Gil, Sang Hee Shim, In Kang Hur
-
Patent number: 8852912Abstract: Described are compositions and methods relating to variant alpha-amylases having altered biochemical properties and advantageous performance characteristics as compared to a reference alpha-amylase. The variants are suitable for use in various industrial applications such as starch conversion, ethanol production, laundry, dishwashing, pulp and paper production, textile desizing, and/or sweetener production.Type: GrantFiled: April 1, 2010Date of Patent: October 7, 2014Assignee: Danisco US Inc.Inventors: David A. Estell, Brian E. Jones, Marc Kolkman, Christian D. Adams, Edward M. Concar
-
Publication number: 20140294846Abstract: The present invention relates to ADAMTS-1 and uses thereof. The present invention also relates to fragments of ADAMTS-1 and methods of inhibiting cell growth and metastasis. The present invention also provide methods of identifying inhibitors and activators relating to the function of ADAMTS-1.Type: ApplicationFiled: February 18, 2014Publication date: October 2, 2014Applicant: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIAInventor: Qin Yu
-
Publication number: 20140295500Abstract: Disclosed are DNA polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases.Type: ApplicationFiled: May 20, 2014Publication date: October 2, 2014Inventors: Keith Bauer, Thomas W. Myers, Shawn Suko
-
Publication number: 20140294842Abstract: A novel class of hydroxylases is described having the amino acid sequence of SEQ ID No. 2, 4, 6 and 8, and variants and fragments thereof having HIF hydroxylation activity. The polypeptides of the invention have in particular prolyl hydroxylase activity. An assay method monitors the interaction of the HIF hydroxylase with a substrate. Modulators of HIF hydroxylase are provided for use in the treatment of a condition associated with increased or decreased HIF levels or activity or for the treatment of a condition where it is desirable to modulate HIF levels or activity.Type: ApplicationFiled: September 16, 2013Publication date: October 2, 2014Applicant: Isis Innovation LimitedInventors: Patrick Henry Maxwell, Christopher William Pugh, Peter John Ratcliffe, Christopher Joseph Schofield
-
Publication number: 20140294773Abstract: A clustered regularly interspaced short palindromic repeat (CRISPR)-associated complex for adaptive antiviral defence (Cascade); the Cascade protein complex comprising at least CRISPR-associated protein subunits Cas7, Cas5 and Cas6 which includes at least one subunit with an additional amino acid sequence possessing nucleic acid or chromatin modifying, visualising, transcription activating or transcription repressing activity. The Cascade complex with additional activity is combined with an RNA molecule to produce a ribonucleoprotein complex. The RNA molecule is selected to have substantial complementarity to a target sequence. Targeted ribonucleoproteins can be used as genetic engineering tools for precise cutting of nucleic acids in homologous recombination, non-homologous end joining, gene modification, gene integration, mutation repair or for their visualisation, transcriptional activation or repression.Type: ApplicationFiled: December 21, 2012Publication date: October 2, 2014Inventors: Stan Johan Jozef Brouns, John Van Der Oost
-
Publication number: 20140296082Abstract: Compositions and methods are described to modify Family B DNA polymerases that contain residual exonuclease activity that interferes with sequencing techniques and with detection of single nucleotide polymorphisms. The compositions are mutant proteins with reduced exonuclease activity compared with presently available “exo?” polymerases, and a sensitive screening assay that enables an assessment of exonuclease activity of any synthetic DNA polymerase.Type: ApplicationFiled: April 8, 2014Publication date: October 2, 2014Applicant: NEW ENGLAND BIOLABS, INC.Inventor: Andrew Gardner
-
Publication number: 20140295027Abstract: The present invention relates to isolated polypeptides having protease activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides in e.g. animal feed and detergents.Type: ApplicationFiled: August 17, 2012Publication date: October 2, 2014Applicant: Novozymes A/SInventors: Carsten Sjoeholm, Peter Rahbek Oestergaard, Tine Hoff, Katrine Pontoppidan, Robert Piotr Olinski
-
Publication number: 20140294801Abstract: The present invention provides an improved and specific mRNA interferase and related methods of protein-based mRNA interference.Type: ApplicationFiled: August 3, 2012Publication date: October 2, 2014Applicant: Rutgers, The State University of New JerseyInventors: Masayori Inouye, Yoshihiro Yamaguchi
-
Publication number: 20140295457Abstract: The present invention refers to the gene cluster and genes comprised by the gene cluster which are involved in the biosynthesis of griselimycin and methylgriselimycin and to the use of the gene cluster, genes comprised thereby and proteins encoded thereby for the production of antibiotic agents.Type: ApplicationFiled: October 11, 2012Publication date: October 2, 2014Inventors: Mark Broenstrup, Claudia Koenig, Luigi Toti, Joachim Wink, Wulf Leuschner, Johann Gassenhuber, Rolf Müller, Silke Wenzel, Tina Binz, Carsten Volz
-
Publication number: 20140298500Abstract: A polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule.Type: ApplicationFiled: May 5, 2014Publication date: October 2, 2014Inventors: Brock Binkowski, Braeden Butler, Lance P. Encell, Frank Fan, Brad Hook, Paul Otto, Gediminas Vidugiris, Susan Wigdal, Kristopher Zimmerman
-
Publication number: 20140295526Abstract: This invention relates to modified polynucleotides encoding modified proteases, and methods for altering the production of proteases in microorganisms. In particular, the present invention relates to methods for altering the expression of proteases in microorganisms, such as Bacillus species. The invention discloses modified polynucleotides, vectors, modified polypeptides, and processes for enhancing the production of proteases.Type: ApplicationFiled: June 16, 2014Publication date: October 2, 2014Applicant: DANISCO US INC.Inventors: Eugenio FERRARI, David A. ESTELL
-
Publication number: 20140298543Abstract: The invention provides compositions and methods for manipulating the exchange of water and/or carbon dioxide (CO2) through plant stomata by controlling CO2 sensor genes. The invention provides compositions and methods for enhancing or optimizing biomass accumulation in a plant. The invention provides compositions and methods for opening or closing a stomatal pore on a guard cell in the epidermis of a plant. The invention provides compositions and methods for increasing or decreasing oxygenation efficiency and/or carbon fixation in a guard cell in the epidermis of a plant by manipulating expression of a ribulose-1,5-bisphosphate carboxylase/oxygenase. The invention provides promoters for regulating expression of a nucleic acid in a plant guard cell.Type: ApplicationFiled: March 5, 2014Publication date: October 2, 2014Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Julian I. SCHROEDER, Maria ISRAELSSON-NORDSTROM, Josef M. KUHN, Yingzhen YANG, Honghong HU, Aurelien BOISSON-DERNIER
-
Publication number: 20140298533Abstract: Disclosed are a method for obtaining highly glyphosate-resistant G1174 gene mutants through artificial mutation and use thereof. Two or more amino acid mutations are introduced in the amino acid sequence from position 95 to position 114 of protein encoded by G1174 gene, and the highly glyphosate-resistant mutants are selected. Also disclosed is a highly glyphosate-resistant mutated G1174 gene, wherein the amino acid sequence from position 95 to position 114 is selected from any one of SEQ ID NO: 4 to SEQ ID NO: 24. The use of the highly glyphosate-resistant mutated gene is for conferring a plant with glyphosate resistance through expression of the transgene in the plant.Type: ApplicationFiled: January 17, 2012Publication date: October 2, 2014Applicant: HANGZHOU RUIFENG BIOTECHNOLOGY LIMITED INC.Inventors: Zhicheng Shen, Chaoyang Lin, Xiaoli Xu, Xinlan Li
-
Publication number: 20140298508Abstract: The present invention is directed to alpha-mannosidase sequences from plants and the use thereof, especially genomic nucleotide sequences containing the regulatory elements controlling their expression, intron and exon sequences and polynucleotide sequences coding for alpha-mannosidase enzymes. Such plants with modified alpha-mannosidase activity can be used for the production of glycoproteins having an altered saccharide composition of great benefit. The present invention also relates to the use of these alpha-mannosidase enzymes for hydrolyzing mannoses.Type: ApplicationFiled: December 23, 2011Publication date: October 2, 2014Applicant: PHILIP MORRIS PRODUCTS S.A.Inventors: Nikolai Valeryevitch Ivanov, Prisca Camponini, Dionisius Florack, Karen Oishi
-
Publication number: 20140298505Abstract: The present invention relates to a nucleic acid molecule encoding (I) a polypeptide having the activity of an endonuclease, which is (a) a nucleic acid molecule encoding a polypeptide comprising or consisting of the amino acid sequence of SEQ ID NO: 1; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 2; (c) a nucleic acid molecule encoding an endonuclease, the amino acid sequence of which is at least 70% identical to the amino acid sequence of SEQ ID NO: 1; (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 50% identical to the nucleotide sequence of SEQ ID NO: 2; (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (d); or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (e) wherein T is replaced by U; (II) a fragment of the polypeptide of (I) having the activity of an endonuclease.Type: ApplicationFiled: June 6, 2012Publication date: October 2, 2014Inventor: Ralf Kühn
-
Publication number: 20140298499Abstract: The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal ?-1,4-xylosidic linkages or endo-?-1,4-glucanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.Type: ApplicationFiled: December 16, 2013Publication date: October 2, 2014Applicant: BP Corporation North America Inc.Inventors: Kevin A. Gray, Reinhard Dirmeier
-
Publication number: 20140294881Abstract: The present invention provides novel protein variants that exhibit reduced immunogenic responses, as compared to the parental proteins. The present invention further provides DNA molecules that encode novel variants, host cells comprising DNA encoding novel variants, as well as methods for making proteins less allergenic. In addition, the present invention provides various compositions that comprise these proteins that are less immunogenic than the wild-type proteins.Type: ApplicationFiled: September 18, 2008Publication date: October 2, 2014Applicant: Danisco US Inc.Inventors: David A. Estell, Fiona A. Harding, Ayrookaran J. Poulose
-
Publication number: 20140295556Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems.Type: ApplicationFiled: March 14, 2014Publication date: October 2, 2014Applicant: THE GENERAL HOSPITAL CORPORATIONInventors: J. Keith Joung, Shengdar Tsai
-
Publication number: 20140296324Abstract: The present disclosure describes nucleic acids, and viruses comprising such nucleic acids, for growing a toxic gene in an insect cell. These nucleic acids comprise a sequence encoding a toxic polypeptide, and an intron that interrupts the sequence, whereby the intron is spliced in mammalian cells but not in insect cells. Infection of mammalian cells but not insect cells with the nucleic acids or viruses can lead to expression of toxic levels of the toxic polypeptide in mammalian cells but not in insect cells. Viruses, such as an AAV or a baculovirus comprising a nucleic acid can be grown in insect cell lines in vitro and can be administered to a subject in need of therapy, such as a subject in need of cancer therapy.Type: ApplicationFiled: October 16, 2012Publication date: October 2, 2014Applicant: Virovek IncorporationInventor: Haifeng Chen
-
Patent number: 8847031Abstract: Thermostable cellulase enzyme systems comprising at least one each of a thermostable endoglucanase, an exo-processive-endoglucanase, and a ?-glucosidase carry out the complete, coordinated hydrolysis of crystalline cellulose to monomeric glucose.Type: GrantFiled: July 9, 2009Date of Patent: September 30, 2014Assignee: The Board of Regents for Oklahoma State UniversityInventors: Rolf A. Prade, Hongliang Wang