Fungal Protein Patents (Class 536/23.74)
  • Patent number: 10934577
    Abstract: Nucleic acid amplification assays for mutations to two short sections of the fungal gene FKS1. Mutations in these target sequences have been shown to correlate with resistance to echinocandin-class drugs. Assays may include detection by sequencing or by labeled hybridization probes. Also, primers, probes and reagent kits for performing such assays.
    Type: Grant
    Filed: May 22, 2017
    Date of Patent: March 2, 2021
    Assignees: RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY, MERCK & CO., INC.
    Inventors: David S. Perlin, Steven Park, Cameron M. Douglas, Jennifer N. Kahn, Stephen A. Parent, Rosemarie Kelly
  • Publication number: 20150113671
    Abstract: The present invention relates to nucleic acids encoding peptides capable of binding to actin. The nucleic acids encoding the peptides are useful in methods for detecting actin in vitro or in living cells.
    Type: Application
    Filed: January 9, 2015
    Publication date: April 23, 2015
    Inventors: Roland Wedlich-Soldner, Michael Sixt, Julia Riedl, Alvaro Crevenna
  • Patent number: 9006514
    Abstract: This invention is in the field of biotechnology, in particular, this pertains to polynucleotide sequences encoding membrane bound O-acyltransferase genes and the use of these acyltransferases for altering fatty acid profiles in oilseed plants. Methods for increasing elongation and desaturation conversion efficiencies are also disclosed.
    Type: Grant
    Filed: December 19, 2013
    Date of Patent: April 14, 2015
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Howard Glenn Damude, Knut Meyer
  • Publication number: 20150093413
    Abstract: The present invention relates to a nucleic acid sequence, comprising or coding for a coding region, encoding at least one peptide or protein comprising a pathogenic antigen or a fragment, variant or derivative thereof, at least one histone stem-loop and a poly(A) sequence or a polyadenylation signal. Furthermore the present invention provides the use of the nucleic acid for increasing the expression of said encoded peptide or protein. It also discloses its use for the preparation of a pharmaceutical composition, especially a vaccine, e.g. for use in the treatment of infectious diseases. The present invention further describes a method for increasing the expression of a peptide or protein comprising a pathogenic antigen or a fragment, variant or derivative thereof, using the nucleic acid comprising or coding for a histone stem-loop and a poly(A) sequence or a polyadenylation signal.
    Type: Application
    Filed: February 15, 2013
    Publication date: April 2, 2015
    Applicant: CureVac GmbH
    Inventors: Andreas Thess, Thomas Schlake, Jochen Probst
  • Patent number: 8993275
    Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: March 31, 2015
    Assignee: Novozymes, Inc.
    Inventor: Nikolaj Spodsberg
  • Patent number: 8993743
    Abstract: The present invention relates to a nucleic acid molecule encoding a chimeric protein having the biochemical activity of a surface active protein, wherein said chimeric protein comprises: (a) an N-terminal portion of a first surface active protein, wherein the N-terminal portion is devoid of between 0 and 10 of the most N-terminal amino acids of the mature first surface active protein; and, C-terminally thereof, (b) a C-terminal portion of a second surface active protein, wherein the C-terminal portion is devoid of between 0 and 10 of the most C-terminal amino acids of the mature second surface active protein. The present invention further relates to a vector, a non-human host and a method for the production of a chimeric protein having the biochemical activity of a surface active protein. In addition, the present invention relates to a chimeric protein encoded by the nucleic acid molecule of the invention and a composition comprising the chimeric protein.
    Type: Grant
    Filed: February 18, 2011
    Date of Patent: March 31, 2015
    Assignee: B.R.A.I.N. Biotechnology Research and Information Network AG
    Inventors: Guido Meurer, Esther Gabor, Anke Bachert, Jürgen Eck
  • Publication number: 20150072380
    Abstract: The present invention relates to the identification of novel nucleic acid sequences, designated herein as 7p, 8k, 7E, 9G, 8Q and 203, in a host cell which effect protein production. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding 7p, 8k, 7E, 9G, 8Q and 203, which are presented in FIG. 1, and are SEQ ID NOS.: 1-6, respectively. The present invention also provides host cells further comprising a nucleic acid encoding a desired heterologous protein such as an enzyme.
    Type: Application
    Filed: September 23, 2014
    Publication date: March 12, 2015
    Applicant: DANISCO US INC.
    Inventors: Elizabeth A. Bodie, Steve Kim
  • Patent number: 8975474
    Abstract: The present invention provides methods and compositions relating to altering NT activity, nitrogen utilization and/or uptake in plants. The invention relates to a method for the production of plants with maintained or increased yield under low or normal nitrogen fertility. The invention provides isolated nitrate transporter (NT) nucleic acids and their encoded proteins. The invention further provides recombinant expression cassettes, host cells, and transgenic plants. Plants transformed with nucleotide sequences encoding the NT enzyme show improved properties, for example, increased yield.
    Type: Grant
    Filed: February 19, 2013
    Date of Patent: March 10, 2015
    Assignee: Pioneer Hi Bred International Inc
    Inventors: Haiyin Wang, Dale F. Loussaert
  • Publication number: 20150038353
    Abstract: The present invention provides a molecular marker for detecting Nosema infection, comprising at least one sequence selected from a gene sequence or a complementary sequence thereof of SR-22, SR-28, SR-71, and SR-85. By designing specific primer sets based on the sequences of molecular marker, the early stage and latent infections of Nosema can be detected, and the infection levels can also be determined.
    Type: Application
    Filed: April 30, 2014
    Publication date: February 5, 2015
    Applicant: National Taiwan University
    Inventors: Chu-Fang LO, Chung-Hsiung WANG, Yu-Shin NAI
  • Patent number: 8946507
    Abstract: A method of producing male or female sterile plants comprising the steps of transforming plant material with a polynucleotide which encodes at least one enzyme which reacts with a non-phytotoxic substance to produce a phytotoxic one, and regenerating the thus transformed material into a plant, wherein the said non-phytotoxic substance is applied to the plant up to the time of male or female gamete formation and/or maturation, so that the non-phytotoxic substance provides for the production of a phytotoxic one which selectively prevents the formation of or otherwise renders the said gametes non-functional, wherein the enzyme is expressed preferentially in either male or female reproductive structures, characterised in that (i) the non-phytotoxic substance is D-phosphinothricin and (ii) the enzyme is a D-amino acid oxidase.
    Type: Grant
    Filed: June 21, 2010
    Date of Patent: February 3, 2015
    Assignee: Syngenta Limited
    Inventors: Timothy Robert Hawkes, Glynn Mitchell, Stephen Thomas Hadfield, Paul Anthony Thompson, Russell Viner, Yan Zhang
  • Publication number: 20150024465
    Abstract: The present invention relates to non-catalytic carbohydrate-binding modules (CBM) belonging to a new family of CBM's. A CBM of the invention was found attached to a glycosyl hydrolase family 61 (GH61) polypeptide and was shown to have little homology with known CBM's indicating that it is the first known member of a new family of CBM's. The present invention further relates to CBM's preferably exhibiting binding affinity for cellulose; to a method of producing such CBM's; and to methods for using such CBM's in the textile, detergent and cellulose fiber processing industries, for purification of polypeptides, immobilisation of active enzymes, baking, manufacturing of biofuel, modification of plant cell walls.
    Type: Application
    Filed: September 29, 2014
    Publication date: January 22, 2015
    Inventors: Kirk Matthew Schnorr, Lars Lehmann Hylling Christensen
  • Publication number: 20140356879
    Abstract: Methods of identifying genes conferring ethanol tolerance in yeasts, genes that confer ethanol tolerance, and mutant strains used to identify such genes are described. A gene herein designated HpETT1 was isolated from the yeast Hansenula polymorpha. Expression of HpETT1 in an ethanol sensitive mutant H. polymorpha strain designated 7E complimented ethanol sensitivity of the mutant. When multiple copies of the HpETT1 were integrated into the genome and overexpressed, the transformed strain demonstrated approximately 10-fold greater resistance to ethanol and resistance to the protein misfolding agent AZC. Expression of HpETT1 also increased ethanol tolerance in Saccharomyces cerevisiae. HpEtt1 has 39% sequence identity to a previously identified protein from S. cerevisiae denoted MPE1, however, the MPE1 gene does not confer ethanol resistance to the 7E mutant.
    Type: Application
    Filed: January 11, 2013
    Publication date: December 4, 2014
    Inventors: Charles Abbas, Andriy Sibirny, Andriy Voronovsky, Olena Ishchuk
  • Publication number: 20140351998
    Abstract: The present invention provides polynucleotides and related polypeptides related to urea uptake. The invention provides genomic sequences for urea transporter, urease and glutamine synthetase genes. Urea transporters, urease and glutamine synthetase are responsible for controlling nitrogen utilization efficiency in plants. Urea transporter, urease or glutamine synthetase sequences are provided for improving grain yield and plant growth. The invention further provides recombinant expression cassettes, host cells and transgenic plants.
    Type: Application
    Filed: November 19, 2013
    Publication date: November 27, 2014
    Applicant: PIONEER HI BRED INTERNATIONAL INC
    Inventors: RAJEEV GUPTA, Zhenglin Hou, Dale F. Loussaert, Bo Shen, Lawrence Kent Wood
  • Patent number: 8877915
    Abstract: Disclosed are: transcription regulatory factors capable of regulating the transcription or expression of genes for mannanases or cellulases, as mentioned below; and others. Specifically disclosed is a protein selected from the following proteins (a), (b) and (c): (a) a protein comprising the amino acid sequence depicted in SEQ ID NO:2; (b) a protein which comprises an amino acid sequence produced by deleting, substituting or adding one or several amino acid residues (e.g., 1 to 5 amino acid residues) in the amino acid sequence depicted in SEQ ID NO:2 and which is capable of regulating the transcription of genes for mannanases or cellulases; and (c) a protein which comprises an amino acid sequence having a 70% or higher sequence identity to the amino acid sequence depicted in SEQ ID NO:2 and which is capable of regulating the transcription of genes for mannanases or cellulases, or a partial fragment of the protein. Also specifically disclosed are a gene encoding the protein, and others.
    Type: Grant
    Filed: March 2, 2010
    Date of Patent: November 4, 2014
    Assignees: National Institute of Advanced Industrial Science and Technology, Noda Institute for Scientific Research
    Inventors: Masahiro Ogawa, Yasuji Koyama, Masayuki Machida
  • Patent number: 8853490
    Abstract: Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for novel variants of antipathogenic polypeptides generated through DNA shuffling that exhibit improved antipathogenic activity. Polynucleotides that encode the antipathogenic polypeptides are also provided. A method for inducing pathogen resistance in a plant using the polynucleotides disclosed herein is further provided. Compositions comprising an antipathogenic polypeptide or a microorganism comprising an antipathogenic polynucleotide of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Plants, plant cells, seeds, and microorganisms comprising an antipathogenic polynucleotide or polypeptide of the invention are also disclosed.
    Type: Grant
    Filed: September 22, 2011
    Date of Patent: October 7, 2014
    Assignee: Pioneer Hi Bred International Inc
    Inventors: James J. English, Azalea S. Ong, Nasser Yalpani
  • Patent number: 8846888
    Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
    Type: Grant
    Filed: October 12, 2012
    Date of Patent: September 30, 2014
    Assignee: Novozymes, Inc.
    Inventors: Kimberly Brown, Paul Harris, Elizabeth Zaretsky, Edward Re, Elena Vlasenko, Keith McFarland, Alfredo Lopez de Leon
  • Publication number: 20140287462
    Abstract: A recombinant filamentous fungal cell (e.g. Aspergillus) having one or more inactivated chromosomal genes is provided. The chromosomal genes in some embodiments correspond to derA, derB, htmA, mnn9, mnn10, ochA, dpp4, dpp5, pepAa, pepAb, pepAc, pepAd, pepF and combinations thereof. The recombinant fungal cells may include further inactivated chromosomal genes which correspond to pepA, pepB, pepC and pepD. The recombinant filamentous fungal cells may include a heterologous nucleic acid encoding a protein of interest. Also provided are methods of producing a protein of interest in said recombinant filamentous fungal cell.
    Type: Application
    Filed: March 18, 2014
    Publication date: September 25, 2014
    Applicant: DANISCO US INC.
    Inventor: Huaming WANG
  • Publication number: 20140283207
    Abstract: The invention relates to novel nucleic acids and their encoded polypeptides from ASR and methods of use that enhance the plant's defensive elicitation response.
    Type: Application
    Filed: March 13, 2013
    Publication date: September 18, 2014
    Applicant: E.I. DU PONT DE NEMOURS AND COMPANY
    Inventors: Karen E. Broglie, Gregory J. Rairdan
  • Publication number: 20140259222
    Abstract: Disease in food crops caused by fungal pathogens is a major concern to the agricultural industry, with annual losses typically in the billions of dollars. Fusarium graminearum, also known as Gibberella zeae, is known to cause, among other diseases, headblight disease in wheat and stalk and ear rot in maize. Disease caused by Fusarium graminearum has proven to be a difficult disease to manage because of limitations of control options. Disclosed herein are nucleic acid sequences which have been proven to provide corn and soybean with resistance to Fusarium graminearum. Also disclosed herein are methods of using the nucleic acid sequences, and plants comprising the nucleic acid sequences.
    Type: Application
    Filed: September 20, 2012
    Publication date: September 11, 2014
    Applicant: Syngenta Participation AG
    Inventors: Xiang Huang, John Daniel Hipskind
  • Patent number: 8828690
    Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding multizymes (i.e., single polypeptides having at least two independent and separable enzymatic activities) along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these multizymes in plants and oleaginous yeast are disclosed.
    Type: Grant
    Filed: April 3, 2008
    Date of Patent: September 9, 2014
    Assignee: E I du Pont de Nemours and Company
    Inventors: Howard G. Damude, Anthony J. Kinney, Kevin G. Ripp, Quinn Qun Zhu
  • Publication number: 20140248689
    Abstract: The present invention relates to a glucose-induced inactivation/degradation-resistant transporter gene and use thereof, and more particularly, to a brewing yeast having excellent assimilation of oligosaccharides (maltose, maltotriose, etc.), an alcoholic beverage prepared using the yeast, a method of producing the alcoholic beverage, etc. More specifically, the present invention relates to a glucose-induced inactivation/degradation-resistant transporter including Mal21p, mutant Mal31p, mutant Mal61p, mutant Mtt1p, mutant Agt1p, etc., a gene encoding the transporter, a method of producing an alcoholic beverage using thereof, and so on.
    Type: Application
    Filed: April 4, 2014
    Publication date: September 4, 2014
    Applicant: SUNTORY HOLDINGS LIMITED
    Inventors: Haruyo HATANAKA, Fumihiko OMURA
  • Publication number: 20140193854
    Abstract: The present invention relates to a method for mass-producing an antifreeze protein derived from a polar yeast, and more particularly, to a method for mass-producing an antifreeze protein derived from Leucosporidium sp., which is the polar yeast, for synthesizing a recombinant polynucleotide by optimizing and altering a gene, which codes the antifreeze protein derived from the polar yeast, for a yeast expression system, and for expressing same using the yeast expression system.
    Type: Application
    Filed: May 17, 2011
    Publication date: July 10, 2014
    Applicant: Korean Ocean Research and Development Institute
    Inventors: Hakjun Kim, Sungho Kang, Junhyuck Lee, Sunggu Lee, Sejong Han, Jongchan Park, Kyoungsun Park
  • Publication number: 20140194367
    Abstract: The isolated Agrocybe aegerita lectin AAL-2 is able to inhibit the growth of tumor cells by inducing apoptosis. In the animal study, the Agrocybe aegerita lectin AAL-2 has been showed to be capable for tumor therapy. Furthermore, the AAL-2 preferentially binds to the carbohydrate or glycoproteins with its N-Acetylglucosamine, which can be used for diagnosing the N-Acetylglucosamine-associated diseases or detecting N-Acetylglucosamine-modified carbohydrates.
    Type: Application
    Filed: October 24, 2011
    Publication date: July 10, 2014
    Applicant: WUHAN ALPHA-OMEGA BIOLOGY TECHNOLOGY CO., LTD(CN)
    Inventors: Hui Sun, Guojun Yu, Yalin Yin, Jinlin Zhang, Shuai Jiang, Bianli Gu, Yijie Chen
  • Publication number: 20140142278
    Abstract: The present invention relates to methods for producing a polypeptide having biological activity, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a first polynucleotide encoding the polypeptide operably linked to a second polynucleotide encoding a variant signal peptide or a variant prepropeptide; and (b) isolating the secreted polypeptide having biological activity from the cultivation medium.
    Type: Application
    Filed: November 15, 2013
    Publication date: May 22, 2014
    Inventors: Mads Bjornvad, Debbie Yaver
  • Publication number: 20140127218
    Abstract: A Candida albicans bloodstream infections cause significant morbidity and mortality in hospitalized patients. Filament formation and adherence to host cells are critical virulence factors of C. albicans. Multiple filamentation regulatory pathways have been discovered, however the downstream effectors of these regulatory pathways remain unknown. The cell surface proteins in the ALS group are downstream effectors of the filamentation regulatory pathway. Particularly, Als1p mediates adherence to endothelial cells in vitro and is required for virulence. The blocking of adherence by the organism is described resulting from the use of a composition and method disclosed herein. Specifically, a pharmaceutical composition comprised of a gene, gene product, or specific antibody to the ALS gene family is administered as a vaccine to generate an immune response capable of blocking adherence of the organism.
    Type: Application
    Filed: September 23, 2013
    Publication date: May 8, 2014
    Inventors: John E. Edwards, JR., Scott G. Filler, Donald C. Sheppard, Ashraf S. Ibrahim, Yue Fu, Brad J. Spellberg
  • Patent number: 8710207
    Abstract: The present disclosure relates, in some embodiments, to compositions, organisms, systems, and methods for expressing a gene product in a plant using a expression control sequence (ECS) operable in monocots and/or dicots. For example, (i) an isolated nucleic acid may comprise an ECS (e.g., a sugarcane bacilliform virus promoter) and, optionally, an exogenous nucleic acid (ExNA) operably linked to the ECS; (ii) an expression vector may comprise an ECS; an ExNA; and, optionally, a 3? termination sequence, wherein the ECS has promoter activity sufficient to express the ExNA in at least one monocot and at least one dicot; (iii) a microorganism, plant cell, or plant may comprise an isolated nucleic acid; (iv) a method for constitutively expressing an ExNA in a plant (e.g.
    Type: Grant
    Filed: May 10, 2011
    Date of Patent: April 29, 2014
    Assignee: The Texas A&M University Systems
    Inventors: T. Erik Mirkov, Jong Won Park, San-Ji Gao
  • Publication number: 20140101793
    Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: December 12, 2013
    Publication date: April 10, 2014
    Applicants: Novoyzmes A/S, Novozymes, Inc.
    Inventor: Marc Dominique Morant
  • Patent number: 8691536
    Abstract: To provide a pectin lyase and a gene for a pectin lyase which can be used in an enzyme preparation for processing plant tissue, and which possesses sufficient maceration activity even under highly acidic conditions. Isolation and purification of a pectin lyase and a gene for a pectin lyase having a molecular weight of 39,500 produced by an Aspergillus filamentous fungus, and possessing a maceration activity and a pectin lyase activity. The pectin lyase has a specified amino acid sequence, an optimal pH of 3.5 for maceration activity, an optimal pH of 4.5 for pectin lyase activity, and deactivates with boiling treatment for 15 minutes at 121° C. Sterilizing heat treatment are unnecessary, because the present invention possesses sufficient maceration activity at pH 3.0-4.0, and the resulting pectin lysate possesses a bactericidal activity and a potent antibacterial activity.
    Type: Grant
    Filed: July 15, 2010
    Date of Patent: April 8, 2014
    Assignees: Bio-I Co., Ltd.
    Inventors: Yoshihisa Nakano, Mitsuhiro Uenoyama, Erina Kobayashi
  • Patent number: 8679801
    Abstract: The invention relates to a nucleic acid sequence encoding an Aspergillus mitochondrial tricarboxylic acid transporter that can be used in the production of itaconic acid in micro-organisms. Preferably said transporter protein is the protein encoded by the nucleic acid which is located on a chromosome segment of A. terreus that also harbors other genes involved in the itaconic acid biosynthesis and the lovastatin biosynthesis. Also, vectors, hosts and transformed micro-organisms are part of the invention.
    Type: Grant
    Filed: February 16, 2009
    Date of Patent: March 25, 2014
    Assignee: Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek TNO
    Inventors: Peter Jan Punt, Maria Johanna Van Der Werf
  • Publication number: 20140080178
    Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: March 9, 2012
    Publication date: March 20, 2014
    Inventors: Kirk Schnorr, Randall Kramer
  • Publication number: 20140082769
    Abstract: The present application belongs to the field of functional peptides and more particularly to the field of controlled protein aggregation. The invention discloses molecules of a peptide structure as defined in the claims and methods of using such molecules for therapeutic applications and for diagnostic uses, as well as in other applications such as in the agbio field and in industrial biotechnology. The molecules can be used for curing and/or stabilizing infections such as bacterial, fungal and viral diseases, but are also useful in non-infectious human and veterinary diseases. The molecules can also be used for the detection of protein biomarkers and for the prognosis and diagnosis of a variety of diseases.
    Type: Application
    Filed: March 12, 2012
    Publication date: March 20, 2014
    Inventors: Joost Schymkowitz, Frederic Rousseau
  • Publication number: 20140075603
    Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: November 14, 2013
    Publication date: March 13, 2014
    Inventors: Lan Tang, Ye Liu, Junxin Duan, Wenping Wu, Randall Kramer
  • Publication number: 20140073008
    Abstract: The invention in principle pertains to the field of recombinant manufacture of alkaloids and, in particular, cycloclavine. It provides polynucleotides for the gene cluster of enzymes involved in the biosynthesis of cycloclavin as well as vectors and host cells comprising such polynucleotides. Also provided are polypeptides encoded by the said polynucleotides which can be applied in a method for the manufacture of cycloclavine also encompassed by the present invention.
    Type: Application
    Filed: February 24, 2012
    Publication date: March 13, 2014
    Applicant: BASF SE
    Inventors: Hartwig Schroeder, Birgit Hoff
  • Patent number: 8642298
    Abstract: The invention relates to a nucleic acid sequence encoding an itaconate transporting Major Facilitator Superfamily Transporter (MFST) gene sequence and the protein encoded thereby. Preferably said sequence is the nucleic acid that comprises the sequence of ATEG_09972.1 of Aspergillus terreus or homologues thereof. Overexpression of the protein enhances the production of itaconic acid in microorganisms.
    Type: Grant
    Filed: March 4, 2009
    Date of Patent: February 4, 2014
    Assignee: Nederlandse Organisatie Voor Toegepast—Natuurwetenschappelijk Onderzoek Tno
    Inventors: Maria Johanna Van Der Werf, Peter Jan Punt
  • Patent number: 8642836
    Abstract: A method of producing male or female sterile plants comprising the steps of transforming plant material with a polynucleotide which encodes at least one enzyme which reacts with a non-phytotoxic substance to produce a phytotoxic one, and regenerating the thus transformed material into a plant, wherein the said non-phytotoxic substance is applied to the plant up to the time of male or female gamete formation and/or maturation, so that the non-phytotoxic substance provides for the production of a phytotoxic one which selectively prevents the formation of or otherwise renders the said gametes non-functional, wherein the enzyme is expressed preferentially in either male or female reproductive structures, characterized in that (i) the non-phytotoxic substance is a D-alpha amino acid, and (ii) the enzyme is a D-amino acid oxidase.
    Type: Grant
    Filed: April 29, 2008
    Date of Patent: February 4, 2014
    Assignee: Syngenta Limited
    Inventors: Timothy Robert Hawkes, Glynn Mitchell, Stephen Thomas Hadfield, Paul Anthony Thompson, Russell Viner, Yan Zhang
  • Patent number: 8637733
    Abstract: This invention is in the field of biotechnology, in particular, this pertains to polynucleotide sequences encoding membrane bound O-acyltransferase genes and the use of these acyltransferases for altering fatty acid profiles in oilseed plants. Methods for increasing elongation and desaturation conversion efficiencies are also disclosed.
    Type: Grant
    Filed: December 16, 2010
    Date of Patent: January 28, 2014
    Assignee: E.I. du Pont de Nemours and Company
    Inventors: Howard Glenn Damude, Knut Meyer
  • Publication number: 20140026261
    Abstract: The present invention provides isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: April 25, 2012
    Publication date: January 23, 2014
    Inventors: Yu Zhang, Lan Tang, Svend Hostgaard Bang Henriksen
  • Publication number: 20140024095
    Abstract: Improved haa1 transcriptional regulatory proteins, polynucleotides encoding improved haa1 transcriptional regulatory proteins and vectors and cells thereof are provided, as well as methods for converting a cellulose-containing biomass feedstock to ethanol using improved haa1 transcriptional regulatory proteins and cells expressing heterologous haa1 transcriptional regulatory proteins as disclosed herein.
    Type: Application
    Filed: July 22, 2013
    Publication date: January 23, 2014
    Applicant: EdeniQ, Inc. (027586)
    Inventors: Kenneth Zahn, Sandra Jacobson
  • Publication number: 20140011262
    Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence and the 3? end of the first nucleotide sequence is immediately upstream of the initiator codon of the second nucleotide sequence. The present invention also relates to the isolated signal peptide sequences and to constructs, vectors, and fungal host cells comprising the signal peptide sequences operably linked to nucleotide sequences encoding polypeptides.
    Type: Application
    Filed: July 18, 2013
    Publication date: January 9, 2014
    Applicant: NOVOZYMES, INC.
    Inventors: Suchindra Maiyuran, Ana Fidantsef, Howard Brody
  • Patent number: 8592649
    Abstract: The present invention provides methods and compositions relating to altering NT activity, nitrogen utilization efficiency and/or uptake in plants. The invention relates to a method for the production of plants with maintained or increased yield under low nitrogen fertility. The invention provides isolated nitrate transporter variant (NT variant) nucleic acids and their encoded proteins. The invention further provides recombinant expression cassettes, host cells, and transgenic plants. Plants transformed with nucleotide sequences encoding the NT variant enzyme show improved properties, for example, increased yield.
    Type: Grant
    Filed: August 20, 2010
    Date of Patent: November 26, 2013
    Assignee: Pioneer Hi Bred International Inc
    Inventors: Lu Liu, Hoa Giang, Dale F. Loussaert, Haiyin Wang
  • Publication number: 20130309765
    Abstract: The present invention provides modified tamabidin 2, which is a modified biotin-binding protein comprising an amino acid sequence represented by SEQ ID NO: 2, an amino acid sequence having one or more amino acid mutations in the amino acid sequence of SEQ ID NO: 2, or an amino acid sequence having an identity of not less than 80% to the amino acid sequence of SEQ ID NO: 2 and having biotin-binding activity, wherein an asparagine residue at position 115 of SEQ ID NO: 2 is replaced with cysteine. The modified biotin-binding protein has remarkable heat resistance.
    Type: Application
    Filed: December 28, 2011
    Publication date: November 21, 2013
    Applicant: JAPAN TOBACCO INC.
    Inventors: Yoshimitsu Takakura, Naomi Oka
  • Publication number: 20130288299
    Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: January 26, 2012
    Publication date: October 31, 2013
    Applicant: Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • Publication number: 20130217076
    Abstract: The saccharification efficiency of cellulase is enhanced within reaction temperature regions of general fermenting microorganisms other than heat-resistant yeast. Cellulosic biomass is saccharified with cellulase in the presence of a polypeptide comprising the amino acid sequence shown in SEQ ID NO: 2 or 4 and having a function of enhancing the saccharifying activity of cellulase.
    Type: Application
    Filed: February 15, 2013
    Publication date: August 22, 2013
    Inventors: Noriko SHISA, Nobuhiro ISHIDA, Chie IMAMURA, Shigeharu MORIYA, Jun KIKUCHI
  • Publication number: 20130217072
    Abstract: The present invention provides for heterologous expression of polypeptides encoded by wild-type and codon-optimized cbh2 genes from the organisms Cochliobolus heterostrophus, Gibberella zeae, Irpex lacteus, Volvariella volvacea, and Piromyces sp. in host cells, such as the yeast Saccharomyces cerevisiae. The expression in such host cells of the corresponding genes, and variants and combinations thereof, result in improved specific activity of the expressed cellobiohydrolases. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.
    Type: Application
    Filed: October 25, 2010
    Publication date: August 22, 2013
    Applicant: Universiteit Stellenbosch
    Inventors: Riaan Den Haan, Emile Van Zyl
  • Publication number: 20130202697
    Abstract: The present invention relates to a nucleic acid molecule encoding a chimeric protein having the biochemical activity of a surface active protein, wherein said chimeric protein comprises: (a) an N-terminal portion of a first surface active protein, wherein the N-terminal portion is devoid of between 0 and 10 of the most N-terminal amino acids of the mature first surface active protein; and, C-terminally thereof, (b) a C-terminal portion of a second surface active protein, wherein the C-terminal portion is devoid of between 0 and 10 of the most C-terminal amino acids of the mature second surface active protein. The present invention further relates to a vector, a non-human host and a method for the production of a chimeric protein having the biochemical activity of a surface active protein. In addition, the present invention relates to a chimeric protein encoded by the nucleic acid molecule of the invention and a composition comprising the chimeric protein.
    Type: Application
    Filed: February 18, 2011
    Publication date: August 8, 2013
    Applicant: B.R.A.I.N. Biotechnology Research and Information Network AG
    Inventors: Guido Meurer, Esther Gabor, Anke Bachert
  • Patent number: 8502026
    Abstract: This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein, enhanced seed oil and modified oil composition. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.
    Type: Grant
    Filed: August 20, 2010
    Date of Patent: August 6, 2013
    Assignee: Monsanto Technology LLC
    Inventors: Maureen Daley, Jason Fenner, Beth Savidge, Dale Val, Wei Zheng
  • Publication number: 20130189733
    Abstract: The present invention relates to a method to improve the secretion of a protein of interest by a filamentous fungal cell comprising inducing a phenotype in the cell selected from the group consisting of a lowered ERAD, an elevated UPR that does not induce an elevated ERAD, wherein ERAD preferably is lowered. The invention further relates to the filamentous fungal cell comprising the phenotype described above. The invention also relates to polynucleotides and polypeptides whose expression can be modulated in the filamentous fungal cell to obtain the above-described phenotype.
    Type: Application
    Filed: January 25, 2013
    Publication date: July 25, 2013
    Applicant: DSM IP ASSETS B.V.
    Inventors: CORNELIS MARIA JACOBUS SAGT, CORNELIS THEODORUS VERRIPS, WALRAVEN HENRY MULLER, NOEL NICOLAAS MARIA ELISABETH VAN PEIJ
  • Publication number: 20130189298
    Abstract: The present invention provides an isolated Saccharomyces pastorianus No 54, which is found to be effective in regulating blood glucose levels and fat or its related disease. Therefore, not only could it control the high blood sugar levels in type 1 diabetic patients, but also it would help to control both of the high blood glucose levels and fat of type 2 diabetic patients.
    Type: Application
    Filed: March 13, 2012
    Publication date: July 25, 2013
    Applicant: GUO-JANE TSAI
    Inventors: GUO-JANE TSAI, Chien-Hui Wu
  • Publication number: 20130189734
    Abstract: The present invention provides isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: November 2, 2011
    Publication date: July 25, 2013
    Applicant: NOVOZYMES INC.
    Inventors: Ye Liu, Lan Tang, Junxin Duan
  • Publication number: 20130183713
    Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: August 30, 2011
    Publication date: July 18, 2013
    Applicants: NOVOYZMES A/S, NOVOZYMES, INC.
    Inventor: Marc Morant