Abstract: The present invention relates to a truncated form of the secretory aspartyl proteinase 2 (Sap2), as well as to nucleic acid molecules encoding same. This truncated Sap2 polypeptide (tSap2) is surprisingly stable and devoid of enzymatic activity but retains full immunogenicity upon intravaginal administration and confers full protection against intravaginal challenge by the Candida fungus. The present invention further relates to compositions comprising tSap2 and to the use of tSap2 in the preparation of such compositions.

Abstract: Method and system for expression systems, based on ade1 and ade2 auxotrophic strains of yeast and fungi, including P. pastoris are disclosed. The expression systems are useful for increased cellular productivity of transformed cell lines and for production of recombinant glycoproteins at industrial scale.

Abstract: Provided herein is an isolated polynucleotide for increasing the alcohol tolerance of a host cell. Also disclosed herein are a vector and a host cell containing the isolated polynucleotide, and a method of increasing the volumetric productivity of a bioalcohol using the same.

Abstract: The present invention relates to compositions and methods comprising genes and peptides associated with cyclic peptide toxins and toxin production in mushrooms. In particular, the present invention relates to using genes and proteins from Amanita species encoding Amanita peptides, specifically relating to amatoxins and phallotoxins. In a preferred embodiment, the present invention also relates to methods for detecting Amanita peptide toxin genes for identifying Amanita peptide-producing mushrooms and for diagnosing suspected cases of mushroom poisoning. Further, the present inventions relate to providing kits for diagnosing and monitoring suspected cases of mushroom poisoning in patients.

Abstract: This invention relates generally to a plant cell with increased tolerance and/or resistance to environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant cell by increasing or generating one or more activities of polypeptides associated with abiotic stress responses and abiotic stress tolerance in plants.

Abstract: An object of the present invention is to search and identify novel antifungal proteins capable of inhibiting the growth of plant pathogenic microorganisms including Magnaporthe grisea and Rhizoctonia solani causing two major rice diseases at relatively low concentrations, and further to clone a gene for said protein. The present invention provides an antifungal protein which can be obtained from fraction(s) precipitated by ammonium sulfate precipitation using an aqueous extract from Pleurotus cornucopiae, wherein said protein has an antifungal activity against at least rice blast, and exhibits existence of a component having a molecular weight of about 15 kDa as determined by SDS-PAGE method; a gene encoding said protein and uses thereof.

Abstract: The present invention relates to ?9 elongases, which have the ability to convert linoleic acid (LA; 18:2 ?-6) to eicosadienoic acid (EDA; 20:2 ?-6) and/or ?-linolenic acid (ALA; 18:3 ?-3) to eicosatrienoic acid (ETrA; 20:3 ?-3). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding ?9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these ?9 elongases in oleaginous yeast are disclosed.

Abstract: The present invention relates to a novel YlMPOl gene which plays an important role in mannosylphosphorylation of an industrial yeast Yarrowia lipolytica, and to a method for preparing a host system capable of producing recombinant glycoproteins free of mannosylphosphate by disruption of the gene. The mannosylphosphorylation is suppressed by the disruption of YlMPOl gene according to the present invention, thereby achieving humanization of glycosylation pathway of Yarrowia lipolytica.

Abstract: The present invention relates to ?8 desaturase genes, which have the ability to convert eicosadienoic acid (EDA; 20:2 ?-6) to dihomo-?-linolenic acid (DGLA; 20:3 ?-6) and/or eicosatrienoic acid (ETrA; 20:3 ?-3) to eicosatetraenoic acid (ETA; (20:3 ?-3). Isolated nucleic acid fragments and recombinant DNA constructs comprising such fragments encoding ?8 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these ?8 desaturases in oleaginous yeast are disclosed.

Abstract: The present invention relates to a polypeptide possessing a CDase activity, characterized in that it is derived from a native CDase by addition of an amino acid sequence with the proviso that said polypeptide has no UPRtase or Thymidine Kinase activity.

Abstract: Described herein are methods for increasing the amount of protein secreted by a cell. In one case, a cell is provided which contains a heterologous nucleic acid encoding a protein having unfolded protein response modulating activity and a heterologous nucleic acid encoding a protein of interest to be secreted. In one case, the protein having unfolded protein response modulating activity is selected from the proteins selected from the group consisting of HAC1, PTC2 and IRE1. The protein of interest can be any secreted protein such as a therapeutic or an industrial enzyme. For example the protein can be selected from the group consisting of lipase, cellulase, endo-glucosidase H, protease, carbohydrase, reductase, oxidase, isomerase, transferase, kinase, phosphatase, alpha-amylase, glucoamylase, lignocellulose hemicellulase, pectinase and ligninase.

Abstract: A novel diglycosidase produced by a microorganism belonging to the genus Penicillium, having the following physicochemical properties: (1) action and substrate specificity: it acts on a disaccharide glycoside, releasing the disaccharide sugar and the aglycone thereof; (2) optimum pH: around 4.5; (3) pH stability: it is stable at pH 4.0 to 8.0 under the processing condition of 37° C. for 30 minutes, and retains its 80% or more of the activity even after processing at pH 4.0 or lower; (4) optimum temperature: around 60° C. in a sodium acetate-acetic acid buffer solution (pH 5.5); (5) thermal stability: it is stable at 50° C. or lower in a sodium acetate-acetic acid buffer solution (pH 5.5) and retains 45% of the activity even after processing at 60° C. for 40 minutes; (6) molecular weight: 40,000±5,000 Da based on SDS-PAGE measurement; and (7) isoelectric point (pI): about 4.3.

Abstract: A mutant Pichia pastoris alcohol oxidase 1 (AOX1) promoter of the wild type Pichia pastoris AOX1 promoter (SEQ ID No. 1) comprising at least one mutation selected from the group consisting of: a) a transcription factor binding site (TFBS), b) nucleotides 170 to 235 (?784 to ?719), nucleotides 170 to 191 (?784 to ?763), nucleotides 192 to 213 (?762 to ?741), nucleotides 192 to 210 (?762 to ?744), nucleotides 207 to 209 (?747 to ?745), nucleotides 214 to 235 (?740 to ?719), nucleotides 304 to 350 (?650 to ?604), nucleotides 364 to 393 (?590 to ?561), nucleotides 434 to 508 (?520 to ?446), nucleotides 509 to 551 (?445 to ?403), nucleotides 552 to 560 (?402 to ?394), nucleotides 585 to 617 (?369 to ?337), nucleotides 621 to 660 (?333 to ?294), nucleotides 625 to 683 (?329 to ?271), nucleotides 736 to 741 (?218 to ?213), nucleotides 737 to 738 (?217 to ?216), nucleotides 726 to 755 (?228 to ?199), nucleotides 784 to 800 (?170 to ?154) or nucleotides 823 to 861 (?131 to ?93) of Seq ID No.

Abstract: Polynucleotides are disclosed which are capable of enhancing a growth, yield under water-limited conditions, and/or increased tolerance to an environmental stress of a plant transformed to contain such polynucleotides. Also provided are methods of using such polynucleotides and transgenic plants and agricultural products, including seeds, containing such polynucleotides as transgenes.

Abstract: The present invention provides novel genes for glycerol-3-phosphate acyltransferase. A nucleic acid comprising the nucleotide sequence shown in SEQ ID NO: 1 or 4 or a fragment thereof.

Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The present invention provides polypeptides with histone H3 lysine 79 methyltransferase activity as well as nucleic acids encoding the same. Also provided are methods of using the polypeptides and nucleic acids of the invention in screening assays to identify compounds of interest. Further provided are diagnostic methods for leukemia and prognostic methods to predict the course of the disease in a subject.

Abstract: The present invention relates to a polypeptide which has a novel specific arabinose transporter function as well as to nucleic acids coding therefore. The invention further relates to host cells, in particular modified yeast strains which contain the coding nucleic acids and express the polypeptide and functionally integrate it into the plasma membrane and are thus able to absorb L-arabinose. When using modified host cells which express additional proteins of the arabinose metabolic pathway, arabinose can be fermented by these cells, in particular into ethanol. The present invention is therefore relevant, inter alia, in connection with the production of biochemicals from biomass, such as bioethanol for example.

Abstract: An L-arabinose utilizing yeast strain is provided for the production of ethanol by introducing and expressing bacterial araA, araB and araD genes. L-arabinose transporters are also introduced into the yeast to enhance the uptake of arabinose. The yeast carries additional genomic mutations enabling it to consume L-arabinose, even as the only carbon source, and to produce ethanol. Methods of producing ethanol include utilizing these modified yeast strains.

Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The present invention pertains to a polynucleotide sequence expressed in Flammulina velutipes against carboxin and the proteins encoded thereby. Also provided is the expression vector and host cell comprising the polynucleotides of the invention.

Abstract: The present invention relates to a method to improve the secretion of a protein of interest by a filamentous fungal cell comprising inducing a phenotype in the cell selected from the group consisting of a lowered ERAD, an elevated UPR that does not induce an elevated ERAD, wherein ERAD preferably is lowered. The invention further relates to the filamentous fungal cell comprising the phenotype described above. The invention also relates to polynucleotides and polypeptides whose expression can be modulated in the filamentous fungal cell to obtain the above-described phenotype.

Abstract: The present invention relates to a detoxifizyme with the activity of transforming aflatoxin and the gene encodes thereof. Firstly a novel protein is isolated and purified, named aflatoxin-detoxifizyme (ADTZ), which has the activity of transforming aflatoxin. The ADTZ gene is obtained through specific primers, and the gene is purified and sequenced. The gene encoding of ADTZ is cloned from the total RNA of Armillariella tabescens. The recombinant protein is expressed and purified through various expression systems using genetic engineering methods. The said detoxifizyme has bioactivity of transforming AFB1, reducing mutagenic effects of AFB1. It has great potential for the manufacturing of feed or food and development of anti-tumor medicament.

Abstract: Novel chemokines and 7 transmembrane receptors from mammals, reagents related thereto, including purified proteins, specific antibodies, and nucleic acids encoding the chemokines and receptors are disclosed. Methods of using the chemokines, receptors, reagents and diagnostic kits are also provided.

Abstract: Provided herein are methods for identifying centromeres and centromeres identified by such methods. Centromeres of organisms such as algae, fungi, and protists can be used, for example, for constructing artificial chromosomes and cells containing such artificial chromosomes.

Abstract: The present invention provides fatty acid synthetases which are responsible for novel fatty acid synthesis, polynucleotides which encode such fatty acid synthetases (e.g., a polynucleotide comprising (a) a polynucleotide consisting of the nucleotide sequence of Positions 1 to 12486 of SEQ ID NO: 1, or (b) a polynucleotide which hybridizes under stringent conditions to a polynucleotide comprising a nucleotide sequence complementary to the nucleotide sequence of Positions 1 to 12486 of SEQ ID NO: 1, and which encodes a protein having a fatty acid synthetase activity), expression vectors and transformants comprising such polynucleotides, methods for producing food and other products using such transformants, food products produced by such methods, and methods for assessing and selecting lipid-producing test fungi.

Abstract: The present invention relates to the production of novel, recombinant polynucleotides comprising the GIR1 ribozyme, or a variant thereof, vectors comprising such polynucleotides and recombinant host cells comprising such polynucleotides and/or such vectors. The invention furthermore relates to the use of said polynucleotides in the treatment of an individual suffering from a disease associated with or caused by instability of a transcript of said second subsequence such as cancer, cachexia, ?-Thallasemia or leukaemia.

Abstract: The present invention relates to a brewery yeast having controlled hydrogen sulfide-producing capability, a process for producing alcoholic beverages with controlled hydrogen sulfide amount. More particularly, the present invention relates to a yeast whose hydrogen sulfide-producing capability that increases the product flavor is controlled by enhancing the expression level of MET17 gene encoding brewery yeast O-acetylhomoserinesulfhydorelace Met17p, particularly non-ScMET17 gene specific to lager brewing yeast, and to a method for producing alcoholic beverages with said yeast.

Abstract: The invention relates to the field of secondary metabolite production in plants and plant cell cultures. More specifically, the invention relates to the use of transporters and more particularly ABC-transporters to enhance the production and/or secretion of secondary metabolites in plants and plant cell cultures.

Abstract: The invention provides a yeast-enhanced red fluorescent protein. In an embodiment of the invention, the yeast-enhanced red fluorescent protein is monomeric and is expressible in Candida albicans. The invention also provides a novel visible color marker for plasmid expression in yeast, particularly Saccharomyces cerevisiae and Candida albicans.

Abstract: The invention relates to yeast strains in which a human GLUT4 transport or a human GLUT1 transporter can be functionally expressed and to particular GLUT4 transport proteins which can be functionally expressed particularly readily in yeast strains.

Abstract: The present invention relates to a glycerol channel gene and its uses, specifically, a brewery yeast producing alcoholic beverages with excellent body and mellowness, alcoholic beverages produced using the yeast, a process for producing the alcoholic beverages. More particularly, the present invention relates to a yeast whose capability of producing glycerol, which contribute to body and mellowness of products, was controlled by controlling expression level of FPS1 gene encoding brewery yeast glycerol channel Fps1p, particularly non-ScFPS1 gene specific to lager brewing yeast, and to a method for producing alcoholic beverages with the yeast.

Abstract: The invention relates to a filamentous fungal cell (e.g., Aspergillus sp.) comprising at least one inactivated protease gene chosen from apsB, a homolog of apsB, cpsA, a homolog cpsA, and combinations thereof. Nucleic acids and methods for making the inactivated mutant filamentous fungal cells are provided as well as methods for using the cells for the altered production of endogenous or heterologous proteins of interest.

Abstract: The invention relates to a method for identifying fungicides, to the use of fungal IPP isomerase for identifying fungicides, and to the use of inhibitors of the IPP isomerase as fungicides.

Abstract: The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein according to the present invention are concerned with (a), (b) or (c), namely (a) a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOs: 2, 4, and 6; (b) a protein that has at least 50% homology to the protein comprising the amino acid sequence of the sequence described in (a) and having saponin-decomposing activity; or (c) a protein comprising a modified amino acid sequence of the sequence described in (a) that has one or more amino acid residues deleted, substituted, inserted, or added and having saponin-decomposing activity.

Abstract: A HWP1 gene sequence or fragment or variants thereof as a target region in a nucleic acid based assay for Candida albicans and an isolated nucleic acid molecule useful as a probe for identifying C. albicans in a sample. A method for the detection of C. albicans in a sample and quantification of HWP1 gene expression in C. albicans is also described.

Abstract: This invention provides a means for enabling high-level secretory production of proteins, in particular proteins having complicated structures such as antibodies, in host cells such as yeast cells. The invention also provides transformed yeast cells having the activated HAC1 gene and the RRBP1 gene and a method for enabling high-level secretory production of foreign proteins using such transformed host cells by inhibiting O-sugar chain formation indigenous to host cells such as yeast cells.

Abstract: Nucleotide or amino acid sequences that may be used in the detection and/or identification for an ochratoxigenic fungus or in the construction of an atoxigenic strain of an ochratoxigenis fungus. The fungus may be of the genus Aspergillus, species carbonarius, niger, alliaceus, or foetidux. The fungus may also be of the genus Penicillium, species verrucosum.

Abstract: The present invention relates to a gene capable of improving low temperature performance (low temperature fermentability and/or low temperature resistance) and use thereof, in particular, to a brewery yeast with superior low temperature performance, alcoholic beverages produced using said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast, whose low temperature performance is improved by amplifying expression level of DLT1 gene encoding Dltlp capable of improving low temperature performance of a brewery yeast, especially non-ScDLT1 gene specific to a lager brewing yeast, and to a method for producing alcoholic beverages using said yeast.

Abstract: The present invention relates to fungal cells and fungi which synthesize hyaluronan and to methods for preparing such fungi, and also to methods for preparing hyaluronan with the aid of these fungal cells or fungi. Furthermore, the present invention relates to the use of fungi for preparing hyaluronan and to food or feed which comprises hyaluronan.

Abstract: An object of the present invention are to provide a protease that is stable in a wide pH range from acidic to alkaline, and that has excellent thrombolytic activity; a protease-producing microorganism that produces the above protease; and a process for producing the protease. By culturing a novel filamentous fungus belonging to the genus Fusarium (Fusarium sp. strain BLB), a protease that is stable in a wide pH range from acidic to alkaline and that has excellent thrombolytic activity, is formed and accumulated in the culture medium, and recovered.

Abstract: The present invention relates to an dihydroxy-acid dehydratase gene and use thereof, in particular, a brewery yeast for producing alcoholic beverages having superior flavor, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast whose level of producing total vicinal diketones, especially level of producing diacetyl, that are responsible off-flavor of the product, is reduced by amplifying the expression level of ILV3 gene encoding brewery yeast dihydroxy-acid dehydratase ILV3p, particularly non-ScILV3 gene specific to lager brewing yeast, and to a method for producing alcoholic beverages with said yeast.

Abstract: Provided herein is Gliocladium isolate C-13 (NRRL 50072) which is an isolated strain of a Gliocladium spp. obtained from an endophyte of a Eucryphia cordifolia plant. Methods of culturing Gliocladium isolate C-13 are provided. The methods can include culturing the Gliocladium isolate C-13 under conditions sufficient to produce hydrocarbons. Also disclosed are methods for producing a hydrocarbon, a hydrocarbon fuel and a blend useful as a fuel. For example, the hydrocarbons produced from isolate C-13 can be used to generate biofuels, such as biofuels for vehicles and aircraft. Also provided are kits which comprise Gliocladium isolate C-13 and methods of cloning, isolating, and expressing Gliocladium isolate C-13 genes.

Abstract: To improve the activity of a Koji mold protease in a solid or liquid culture medium in the production of foods (e.g., a seasoning), pharmaceuticals (e.g., a digestive agent), protease for use in a detergent and the like. Disclosed are a recombinant vector having capability of increasing the secretion of the Koji mold protease, a Koji mold which is transformed with the vector and has an increased expression of a gene for a protease or an increase secretion of the same, a method for the production of a protease by using the transformed Koji mold, and the like.

Abstract: A nucleic acid construct or construct system which includes (i) a first polynucleotide encoding endochitinase, (ii) a second polynucleotide encoding stilbene synthase and (iii) a third polynucleotide encoding superoxide dismutase. In addition, banana cells transformed with the nucleic acid construct or construct system, banana plants generated therefrom and methods of producing same.

Abstract: A novel Candida albicans nucleotide and polypeptide, CaSRF1, involved in regulating the morphogenetic transformation and virulence in response to engulfment by the immune response cells of its model host is described. The gene is unique in its ability to affect the virulence-associated morphogenesis in vivo but is not required for the morphogenesis in vitro. The putative membrane localization and its effect on cell wall integrity indicates that it is an ideal anti-candida drug target by virtue of its predicted easy accessibility to lead molecules/chemicals and its ability to affect virulence.

Abstract: The present invention relates to a gene encoding trehalose-6-phosphate phosphatase and use thereof, in particular, a yeast for practical use with superior resistance property to dryness and/or low-temperature storage, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast, whose resistance property to dryness and/or resistance property to low-temperature storage is enhanced by amplifying expression level of TPS2 gene encoding a trehalose-6-phosphate phosphatase Tps2p in brewer's yeast, especially non-ScTPS2 gene specific to a lager brewing yeast and to a method for producing alcoholic beverages with said yeast, etc.

Abstract: In Magnaporthe species and other plant pathogenic fungal species, the appressoriu, (infection structure) is responsible for breaching the host plant cell wall and gaining entry into the host tissues. Magnaporthe ABC3 protein is an MDR transporter that plays an important role during host penetration and also is involved in regulating fungal response to intracellular oxidative stress. The insertional mutant abc3?, in which the ABC3 efflux pump function is blocked, lacks functional appressoria and is therefore incapable of causing disease in host plants. This invention provides the abc3 nucleic acid (gene) and ABC3 protein from Magnaporthe or from Aspergillus, Ustilago or Fusarium and describes methods for reducing plant pathogenicity for important rice pathogens.

Abstract: A process for expression of a protein product in Aspergillus oryzae is disclosed. The process comprises transforming Aspergillus oryzae with a vector system comprising DNA-sequences encoding functions facilitating gene expression, a suitable marker for selection of transformants, and a DNA-sequence encoding the desired protein product. The process enables industrial production of many different polypeptides and proteins in A. oryzae. Examples of such products are chymosin or prochymosin other rennets, proteases, lipases and amylases. Also disclosed is an effective promoter for expression of a protein in Aspergillus. A preferred promoter is the TAKA-amylase promoter or functional parts thereof. There is also provided a process for the production of a recombinant Humicola lipase. The recombinant Humicola lipase from A. oryzae differs from the native lipase in having a greater glycosylation and in exhibiting an improved thermostability.

Abstract: The present invention relates to an acetolactate synthase gene and use thereof, in particular, a brewery yeast for producing alcoholic beverages with superior flavor, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast, whose capability of producing vicinal diketones, especially diacetyl, that are responsible for off-flavors in products, is reduced by repressing expression level of ILV2 gene encoding an acetolactate synthase (Ilv2p), especially non-ScILV2 gene specific to a lager brewing yeast, and to a method for producing alcoholic beverages with said yeast.