Abstract: The present invention claims methods for the stable integration of exogenous DNA into a specific locus, E32, in the maize genome through the use of zinc finger nucleases. Maize plants and plant parts that were transformed by the methods of the invention are claimed. The invention is useful for creating desirable traits such as herbicide resistance, herbicide tolerance, insect resistance, insect tolerance, disease resistance, disease tolerance, stress tolerance, and stress resistance in maize The E32 locus represents a superior site for inserting foreign genes because native agronomic phenotypes are not disturbed.

Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements obtained from Brassica napus.

Abstract: The invention relates to the alfalfa variety designated 412W201. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety 412W201. Also provided by the invention are tissue cultures of the alfalfa variety 412W201 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety 412W201 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The present disclosure relates to recombinant proteins that induce epigenetic gene silencing and to methods of using such proteins for reducing the expression of genes in plants.

Abstract: A method of increasing expression of a product encoded by a nucleic acid molecule is provided where in one embodiment multiple plant transcription units comprising a promoter and the nucleic acid molecule are provided. In another embodiment multiple plant transcription units are provided where each promoter is different from the other. In an embodiment the multiple plant transcription units may comprise two, three, four or more plant transcription units. In another embodiment the promoter may be selected from an embryo promoter. Another embodiment provides the promoter may be a globulin promoter. A further embodiment provides the product encoded may be selected from hepatitis b, aprotinin, or a cellulase. Still further embodiments provide the product may be selected from the cellulases E1 and CBH1.

Abstract: The present invention generally relates to the use of synergistic amounts of Bacillus thuringiensis subsp. aizawai and chlorantraniliprole for the control of Diamondback Moth, Beet Armyworm, Soybean Looper, Corn Earworm, Cabbage Looper, and Southwestern Corn Borer. Specifically, the synergistic ratio of Bacillus thuringiensis subsp. aizawai to chlorantraniliprole is from about 1:0.00003 to about 1:3.

Abstract: The disclosure provides materials and methods for conferring improved plant traits or benefits on plants. The materials can include a formulation comprising an exogenous endophytic bacterial population, which can be disposed on an exterior surface of a seed or seedling, typically in an amount effective to colonize the plant. The formulations can include at least one member selected from the group consisting of an agriculturally compatible carrier, a tackifier, a microbial stabilizer, a fungicide, an antibacterial agent, an herbicide, a nematicide, an insecticide, a plant growth regulator, a rodenticide, and a nutrient.

Abstract: The present invention generally relates to the use of synergistic amounts of Bacillus thuringiensis subsp. aizawai, Bacillus thuringiensis subsp. kurstaki and chlorantraniliprole for the control of diamondback moth, beet armyworm, sugarcane borer, soybean looper, corn earworm, cabbage looper and southwestern corn borer, wherein the ratio of Bacillus thuringiensis subsp. kurstaki to Bacillus thuringiensis subsp. aizawai is from about 1:0.47 to about 1:0.92, and the ratio of the total amount of Bacillus thuringiensis to chlorantraniliprole is from about 1:0.0001 to about 1:20.

Abstract: Compositions and methods for the prevention and treatment of anxiety conditions associated with the use of cannabis are disclosed herein. The medicinal compositions include cannabidiol or cannabidiolic acid and one or more of the following compounds: bergamottin or dihydroxybergamottin, phloretin or a phloretin glycoside, piperine, apigenin or an apigenin glycoside and ursolic acid.

Abstract: A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-?-sheet junction or a loop-?-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.

Abstract: A method for cultivating a plant includes applying allantoin to a plant; exposing the plant to a temperature of about 30° C. or more for at least about 60 minutes; and growing the plant in a cultivation medium.

Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: Isolated nucleotide sequences mutated by insertion encoding a truncated sunflower oleate desaturase protein, truncated protein, methods, procedures and uses. The isolated nucleotide sequences comprise an insertion that includes a stop codon, and wherein the sequences encode a truncated sunflower oleate desaturase protein. The truncated sunflower oleate desaturase protein may be for example the sequence shown in SEQ ID No: 1 or SEQ ID No: 2.

Abstract: By combination of hydrophobic chromatography and strongly basic anion-exchange chromatography, a novel, highly hydrophobic ?-glucosidase was successfully identified from Acremonium cellulolyticus. Further, a gene corresponding to the identified ?-glucosidase was isolated. When multiple modifications were introduced into the base sequence of the gene, the gene was successfully expressed in Trichoderma viride at a high level, and the expression product successfully exhibited a high ?-glucosidase activity.

Abstract: Provided are nucleic acid constructs which comprise a polynucleotide encoding adenosine phosphate-isopentenyltransferase (IPT) operably linked to a stress-related promoter. Also provided are host cells transformed with the nucleic acid constructs, transgenic plants expressing the IPT coding sequence under the transcriptional regulation of a stress-related promoter and methods of using same for increasing abiotic stress tolerance of a plant, and biomass of plants under stress or normal conditions.

Abstract: The invention provides plants having desirable levels of sugar release and/or lignin synthesis. The invention further provides methods of selecting plants with such desirable levels of sugar release and/or lignin synthesis; methods of genetically modifying plants to modulate lignin synthesis, sugar release, and modulating phenylalanine, tyrosine, tryptophan and flavonoid production; and uses of such plants.

Abstract: The present invention provides a polynucleotide which enables the modulation of the seed vigor, and in particular enhances the seed vigor, and more particularly enables the modification of the speed of germination. A plant seed comprising the said polynucleotide is also provided. A method of producing the plant seed, method for improving the germination and vigor of plant seed, transgenic plant and the use of the polynucleotide of the invention for producing plants growing seeds with improved germination and vigor characteristics are also provided. The invention particularly concerns Brassica, more particularly Brassica oleracea.

Abstract: A method for producing a plant with enhanced growth is provided including a step of introducing a gene encoding a chimeric myosin XI protein into a host plant so as to transform the host plant. The chimeric myosin XI protein comprises a neck domain, a coiled-coil domain, and a globular tail domain from a myosin XI protein involved in cytoplasmic streaming of a donor plant. The motor domain from a myosin XI protein of a plant belongs to the genus Chara. The motor domain comprises an amino acid sequence specified in the following (a) or (b): (a) the amino acid sequence shown in SEQ ID NO: 1; or (b) an amino acid sequence having 90% or more identity with the amino acid sequence shown in SEQ ID NO: 1. The host plant belongs to the same family as the donor plant.

Abstract: Provided is a polynucleotide encoding a protein that exhibits activity for transferring a sugar to a hydroxyl group at position 7 of a flavone, particularly flavone 4?-glucoside, wherein the polynucleotide is selected from the group consisting of: (a) a polynucleotides comprising the base sequence of SEQ ID NO: 1 or SEQ ID NO: 5; (b) a polynucleotide that hybridizes, under stringent conditions, with a polynucleotide comprising the base sequence complementary to the base sequence of SEQ ID NO: 1 or SEQ ID NO: 5; (c) a polynucleotide encoding a protein comprising the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 6; (d) a polynucleotide encoding a protein comprising an amino acid sequence in which one or more amino acids have been deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 6.

Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desaturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.

Abstract: The invention provides siRNA compositions that specifically downregulates expression of a variant of the PNPLA3 gene and methods of use thereof for treating a chronic liver disease or alcoholic liver disease (ALD).

Abstract: The present disclosure provides affinity tags, fusion proteins comprising one or more affinity tags, compositions comprising a fusion protein, methods of purifying a protein using an affinity tag, and devices for purifying a protein using an affinity tag.

Abstract: The Zea mays c.v. B73 Ubiquitin-1 (Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Z. mays genotype, Z. mays c.v. B104.

Abstract: The present invention relates to vaccines that are made in transgenic soybeans for use in humans, animals of agricultural importance, pets, and wildlife. These vaccines are used as vaccines against viral, bacterial, fungal, parasitic or prion related diseases, cancer antigens, toxins, and autologous or self proteins. The transgenic soybeans of the instant invention also can be used for inducing tolerance to allergens or tolerance to autoimmune antigens, wherein an individual shows hypersensitivity to said allergen or has developed autoimmunity to autologous or self proteins, respectively. The invention also relates to prophylatically treating individuals and/or populations prior to showing hypersensitivity to allergens. Other aspects of the invention include using the transgenic soybeans as an oral contraceptive, and the expression of protein adjuvants in transgenic soybeans.

Abstract: The present invention relates to isolated polypeptides having beta-xylosidase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: Transcription regulatory elements, namely promoter and terminator sequences, obtained from Sorghum bicolor that drive RNA transcription predominately in root hair cells are described, as well as cassettes, expression vectors, and genetically modified plants containing these transcription regulatory elements. The genetically modified plants can be gymnosperms, dicots, or monocots. Methods of directing transcription of a heterologous polynucleotide under control of these transcription regulatory elements in a genetically modified plant's root hair cells are also provided.

Abstract: The present invention relates to methods for degrading or converting a cellulosic material and for producing a substance from a cellulosic material.

Abstract: Engineered polypeptides useful in synthesizing acyl amino acids are provided. Also provided are methods of making acyl amino acids using engineered polypeptides.

Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The present invention provides methods and compositions for regulation of gene expression in plants. In particular, the invention provides nucleic acids that can confer tissue specific and constitutive expression to operably linked polynucleotides of interest.

Abstract: A combined packing and assembly method that efficiently packs ribonucleic acid (RNA) into virus like particles (VLPs) has been developed. The VLPs can spontaneously assemble and load RNA in vivo, efficiently packaging specifically designed RNAs at high densities and with high purity. In some embodiments the RNA is capable of interference activity, or is a precursor of a RNA capable of causing interference activity. Compositions and methods for the efficient expression, production and purification of VLP-RNAs are provided. VLP-RNAs can be used for the storage of RNA for long periods, and provide the ability to deliver RNA in stable form that is readily taken up by cells.

Abstract: A hyperthermostable endoglucanase, having an endoglucanase catalytic domain including: (A) a polypeptide including the amino acid sequence represented by SEQ ID NO: 1 or 2, (B) a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1 or 2, and having hydrolysis activity against a substrate of carboxymethyl cellulose at least under conditions of 90° C. and pH 5.5, or (C) a polypeptide including an amino acid sequence having 80% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1 or 2, and having hydrolysis activity against a substrate of carboxymethyl cellulose at least under conditions of 90° C. and pH 5.5.

Abstract: A hyperthermostable endoglucanase, having an endoglucanase catalytic domain including: (A) a polypeptide including an amino acid sequence represented by SEQ ID NO: 1; (B) a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of carboxymethyl cellulose at least under conditions of a temperature of 110° C. and a pH of 5.5; or (C) a polypeptide including an amino acid sequence having at least 55% sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of carboxymethyl cellulose at least under conditions of a temperature of 110° C. and a pH of 5.5.

Abstract: The invention provides DNA molecules and constructs, including their nucleotide sequences, useful for modulating gene expression in plants and plant cells. Transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the DNA molecules operably linked to heterologous transcribable polynucleotides are also provided, as are methods of their use.

Abstract: The invention provides promoter sequences that regulate specific expression of operably linked sequences in developing xylem cells and/or in developing xylem tissue. The developing xylem-specific sequences are exemplified by the DX5, DX8, DX11, and DX15 promoters, portions thereof, and homologs thereof. The invention further provides expression vectors, cells, tissues and plants that contain the invention's sequences. The compositions of the invention and methods of using them are useful in, for example, improving the quantity (biomass) and/or the quality (wood density, lignin content, sugar content etc.) of expressed biomass feedstock products that may be used for bioenergy, biorefinary, and generating wood products such as pulp, paper, and solid wood.

Abstract: Genetic constructs capable of manipulating fructan biosynthesis in photosynthetic cells of a plant, said genetic constructs include a promoter operatively linked to a nucleic acid encoding a bacterial FT enzyme. These constructs can be used in modification of fructan biosynthesis in plants and, more particularly, for manipulating fructan biosynthesis in photosynthetic cells. The constructs can also be used for increasing plant biomass and, more particularly, for enhancing biomass yield and/or yield stability, including shoot and/or root growth in a plant, and for enhancing the productivity of biochemical pathways.

Abstract: A system and method for creating a botanical extract. The system comprising a grow light optimized to stimulate growth of the selected plant and an extractor using supercritical CO2 to obtain the extract.

Abstract: The present application relates to novel heterocyclic compounds, to the use thereof for controlling animal pests including arthropods, insects and nematodes, and to processes and intermediates for preparation of the heterocyclic compounds.

Abstract: A pesticidal composition comprises a synergistically effective amount of a nicotinic acetylcholine receptor (nAChR) allosteric activator compound and a pesticide selected from N-(3-chloro-1-(pyridin-3-yl)-1H-pyrazol-4-yl)-N-ethyl-3-((3,3,3-trifluoropropyl)thio) propanamide (I), N-(3-chloro-1-(pyridine-3-yl)-1H-pyrazol-4-yl)-N-ethyl-3-((3,3,3-trifluoropropyl)sulfinyl) propanamide (II), or any agriculturally acceptable salt thereof. A method of controlling pests comprises applying the pesticidal composition near a population of pests. A method of protecting a plant from infestation and attack by insects comprises contacting the plant with the synergistic pesticidal composition.

Abstract: The present invention generally relates to the use of synergistic amounts of Bacillus thuringiensis subsp. aizawai, Bacillus thuringiensis subsp. kurstaki and chlorantraniliprole for the control of diamondback moth, beet armyworm, sugarcane borer, soybean looper, corn earworm, cabbage looper and southwestern corn borer, wherein the ratio of Bacillus thuringiensis subsp. kurstaki to Bacillus thuringiensis subsp. aizawai is from about 1:0.47 to about 1:0.92, and the ratio of the total amount of Bacillus thuringiensis to chlorantraniliprole is from about 1:0.0001 to about 1:20.

Abstract: The present invention generally relates to the use of synergistic amounts of Bacillus thuringiensis subsp. aizawai and chlorantraniliprole for the control of Diamondback Moth, Beet Armyworm, Soybean Looper, Corn Earworm, Cabbage Looper, and Southwestern Corn Borer. Specifically, the synergistic ratio of Bacillus thuringiensis subsp. aizawai to chlorantraniliprole is from about 1:0.00003 to about 1:3.

Abstract: Provided are isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The present invention provides a transgenic alfalfa event KK 179-2. The invention also provides cells, plant parts, seeds, plants, commodity products related to the event, and DNA molecules that are unique to the event and were created by the insertion of transgenic DNA into the genome of a alfalfa plant. The invention further provides methods for detecting the presence of said alfalfa event nucleotide sequences in a sample, probes and primers for use in detecting nucleotide sequences that are diagnostic for the presence of said alfalfa event.

Abstract: The present invention relates to a method for producing a liquid non-aqueous emulsifiable formulation comprising a pyripyropene pesticide of the formula (I) as defined below which is dissolved in an aromatic hydrocarbon or hydrocarbon mixture containing surfactants, which stabilize the emulsion upon dilution with water, which method comprises the steps i) and ii) as described hereinafter: i) dissolving the at least one surfactant and the compound of formula (I) in the organic solvent or solvent mixture to obtain a clear solution of the at least one surfactant and the compound of the formula (I); ii) heating the solution to a temperature of at least 40° C.

Abstract: This application describes and claims a method of improving the health and vigor of a plant, comprising administering to the plant an effective amount of a bacterial composition which comprises a botanically compatible vehicle and an isolated bacterial strain selected from the group consisting of Bacillus megaterium PT6, Bacillus subtilis PT26A, Paenibacillus sp. ATY16, and any combination thereof. Improvement in health and vigor is one or more of the following: a) improved resistance to disease; b) improved ability to defend against disease; c) reduction of disease symptoms; d) faster growth; e) improved crop productivity; f) improved crop quality; g) improved seed germination; h) improved seedling emergence; or any combination thereof. Isolated bacterial strains and mixtures of bacteria are provided, as well as compositions comprising the isolated bacterial strains.

Abstract: ‘PA6UNIBO’ is a new cherry tree variety selected for its vigorous growth and excellent yield, as well as its attractive dark red, flavorful fruit. ‘PA6UNIBO’ cherry ripens 10 to 12 days after ‘Burlat’ cherry.

Abstract: An object of the present invention is to provide DNA of a glycoalkaloid biosynthetic enzyme of a solanaceous plant (Solanaceae) such as a potato. The present invention relates to a protein having glycoalkaloid biosynthetic enzyme activity of a solanaceous plant such as a potato and a method for producing/detecting a novel organism using a gene encoding the protein.

Abstract: Plants having increased resistance to a pest relative to corresponding control plants are provided. The resistant plants have inhibited expression, activity, or function of a susceptibility gene, and thereby have decreased expression, activity, or function of the susceptibility gene in the presence of the pest as compared to a control plant in which the wild-type susceptibility gene is normally expressed in the presence of the pest. Methods of producing plants with increased resistance are also provided. The methods involve altering the expression, activity, or function of a susceptibility gene, such as Mds-1, in the plant. Isolated Mds-1 sequences are provided that can be manipulated to enhance pest resistance in modified plants. Modified plants, plant cells, tissues, seeds, and progeny are also provided having enhanced pest resistance.

Abstract: The present invention relates to a biscuit comprising guar gum characterized in that said guar gum is a native guar gum with a length between 0.25 and 8 mm, a width between 0.18 and 2 mm and an average length to width ratio between 1.8 and 6. The invention also relates to the use of said biscuit to help control the blood glucose level in healthy subjects or in subjects suffering from insulino-resistance or diabetes and especially type-2 diabetes and/or to actively lower blood LDL-cholesterol concentration or to maintain normal blood cholesterol concentrations for healthy subjects or subjects at risk of cardiovascular disease. The invention also relates to the use of said biscuit to increase the feelings of satiety, and/or to decrease the food intake and/or to contribute to weight management.