Abstract: An observation system includes: a container holding unit; a ring illumination, including a light source in a ring shape, arranged in a position opposed to an outer bottom surface of a container so that a central axis of the illumination is aligned to that of a bottom surface of the container held by the container holding unit, when an observation target is observed; a first light-shielding plate in a ring shape, having an inner diameter capable of varying, arranged between the illumination and the container holding unit, and configured to shield light from the illumination; a lens, arranged in a position opposed to an inner bottom surface of the container held by the container holding unit, to observe the observation target; and a second light-shielding plate, having an outer diameter capable of varying, arranged in an internal space of the illumination, and configured to shield light from the illumination.
Abstract: An illuminating system (21) is provided for an optical viewing apparatus (1) which can be operated in a fluorescence mode. The illuminating system includes at least one broadband light source (31) for illuminating a viewed object (3) and at least one narrowband light source (37) for exciting fluorescence in the viewed object (3) and/or for background illumination in the fluorescence mode. The illuminating system further includes a light conductor (23) having a light source end inlet end (49) and an outlet end (25) facing toward the viewed object. Furthermore, the illuminating system (21, 210) includes a superposer (43) for superposing the light of the narrowband light source (37) with the light of the broadband light source (31). The superposer (43) is mounted at the inlet end (49) or at the light source side ahead of the inlet end (49) of the light conductor (23).
Type:
Grant
Filed:
June 14, 2010
Date of Patent:
May 5, 2015
Assignee:
Carl Zeiss Meditec AG
Inventors:
Peter Reimer, Markus Bausewein, Helge Jess
Abstract: A conveying device including: a storage unit storing two or more sheets of slide glasses to be subjected to a predetermined treatment; a stage holding only one sheet of slide glass to be subjected to the treatment; a supply arm by which one sheet of slide glass to be subjected to the treatment is picked up from the storage unit and supplied onto the stage; a discharge arm by which the slide glass mounted on the stage is picked up and discharged in the storage unit; a moving unit operable to move the supply arm and the discharge arm in an integral manner so as to bring the supply arm or the discharge arm into proximity to each of the storage unit and the stage; and a control unit operable to control the supply arm, the discharge arm and the moving unit.
Abstract: A microscope includes: a first epi-illumination light-source unit to perform fluorescence observation; a second transmitted-illumination light-source unit to perform transmission observation, the second transmitted-illumination light-source unit including a light source provided with a light emitting element that emits excitation light and a fluorescent substance that emits fluorescence upon irradiation with the excitation light; and an incidence limiting section configured to limit an incidence of light on the light source from an outside of the second light source unit during a light-off period of the light emitting element. The incidence limiting section is configured to remove an incidence limitation of the light from the outside while the light emitting element is being lit.
Abstract: An imaging apparatus is disclosed which uses a super-oscillatory lens to obtain sub-diffraction limit resolution. The super-oscillatory lens is arranged to receive a light beam from a light source, the lens having a pre-defined pattern to spatially modulate the light beam in amplitude and/or phase so that it focuses the light beam to a focus at a first focal point having a full width half maximum of less than half the wavelength. Collection optical elements are arranged to focus the first focal point to a second focal point conjugate to the first focal point. An object for imaging is scanned over the first focal point and a detector is arranged to collect light from a collection region centered on the second focal point.
Type:
Grant
Filed:
March 8, 2012
Date of Patent:
April 14, 2015
Assignee:
University of Southampton
Inventors:
Edward Thomas Foss Rogers, Nikolay Ivanovich Zheludev, John E Chad
Abstract: In an optical measuring device, the visual observation section includes: a white light source which emits white light; a first objective lens arranged between the white light source and measurement object, through which the white light emitted from the white light source and return light from the measurement object transmit; a plurality of tube lenses which change a magnification of the return light passing through the first objective lens to a predetermined magnification; and a lens switching mechanism which can selectively switch the tube lenses so as to select one of the tube lenses to be arranged on the return light, and the special observation section includes: a special light source which emits special light; and a second objective lens arranged between the special light source and measurement object, through which the special light emitted from the special light source and return light from the measurement object transmit.
Abstract: Provided is a microscope optical system in which the occurrence of flare due to unnecessary-order diffracted light exited from a diffractive optical element is suppressed. A microscope objective lens MS is configured by including an objective lens OL which has a diffractive optical element GD and converts light from an object into a substantially parallel light flux, and a second objective lens IL which forms an image of the object by focusing the substantially parallel light flux from the objective lens OL, and is configured such that, in case where an m-th order of diffracted light from the diffractive optical element GD is used for the image formation, the following expression is satisfied: |?|>tan?1(0.06/D) when the light of a maximum NA emitted from the object located on an optical axis enters the diffractive optical element.
Abstract: A device for attaching a first optical microscope component part to a second part includes an annular receptacle attached to the second part and an annual insertion part. The annular insertion part includes outside-conical retaining projections. The receptacle includes a base ring having a retaining collar with an inside cone tapering away from the base ring and in which a lateral opening is formed, through which the insertion part can be inserted in the receptacle such that it is located on a pre-locking position in which the annular openings overlap. The insertion part and the receptacle can be moved by reciprocal rotation from the pre-locking position into a locking position in which the outside-conical retaining projections of the insertion part are seated against the inside cone of the retaining collar and press the insertion part on the base ring, whereby the insertion part is locked to the receptacle.
Type:
Grant
Filed:
February 4, 2011
Date of Patent:
April 7, 2015
Assignee:
Carl Zeiss Microscopy GmbH
Inventors:
Ingo Fahlbusch, Werner Kleinschmidt, Heinrich Bunger, Peter Schnüll, Wolfgang Hartje, Georg Herbst
Abstract: An eyepiece includes a focusing apparatus for positioning the area perceived as in focus by the relaxed human eye and for correcting axial ametropia of an eye of a user of the eyepiece and a correction apparatus for adjustable correction of an astigmatism of an eye of a user of the eyepiece.
Abstract: The invention allows a quantitative evaluation of images acquired by microscope having fewer errors and is applicable in connection with high-resolution methods, particular at a high speed. A microscope image is analyzed in which the intensity distributions of fluorescence events have in each instance a diffraction-dependent extent which corresponds to an extent of a point spread function of the microscope and are arranged so as to be spatially non-overlapping, or at least predominantly spatially non-overlapping, in that at least one counter is initialized for every region to be analyzed in the microscope image, at least one fluorescence event is identified in a region to be analyzed in the microscope image, and the counter corresponding to the relevant region is incremented for each fluorescence event identified in the region. The counting results in a dramatic improvement in the signal-to-noise ratio at a high evaluation speed.
Type:
Grant
Filed:
June 19, 2010
Date of Patent:
March 24, 2015
Assignee:
Carl Zeiss Microscopy GmbH
Inventors:
Ingo Kleppe, Thomas Kalkbrenner, Ralf Wolleschensky
Abstract: A microscope includes: an objective turret holding a micro and a macro objective for rotation to operational positions along an optical axis; observation optics in an imaging beam path, and an illumination device including a beam splitter for generating an illumination beam path and coupling the illumination beam path into the imaging beam path, the macro objective including a first subsystem attachable to the objective turret, and a second subsystem insertable into the imaging beam path between the turret and the observation optics when the first subsystem is operational, the illumination device allowing a telecentric beam path with an illumination pupil produced by either the micro objective or the macro objective, and adjustment optics in the illumination beam path having positive refractive power and causing the illumination pupil to be shifted to a rear exit pupil, located between the first optical subsystem and the beam splitter, of the macro objective.
Abstract: A device for focusing a microscope objective on a sample accurately with a high spatial resolution. The device has a positioning unit having a main body, an objective holder movably supported on the main body and adapted to hold the microscope objective, and an actuator for moving the objective holder along the optical axis of the microscope objective. The objective holder holds the microscope objective only at a front portion of the microscope objective facing the sample. The positioning unit includes one or more lever arms, each of which coupled at its one end via a first flexure bearing to the main body and at its other end via a second flexure bearing to the objective holder. The main body of the positioning unit is attached to a stage that carries the sample.
Abstract: Observation over a superwide field of view is possible with a short, lightweight, and inexpensive eyepiece optical system and a binocular barrel. A microscope optical system includes an objective optical system configured to collect light from a specimen; an imaging optical system configured to image the light coming from the specimen and collected by the objective optical system; and an eyepiece optical system configured to magnify the image of the specimen formed by the imaging optical system and form a virtual image in an eye of an observer, wherein the following conditional expressions are satisfied, M=Fntl/Fob×250/Fne??(1) Fntl=Ftl×Kt??(2) Fne=Fe×Kt??(3) 0.4<kt<0.95??(4).
Abstract: A Raman microspectrometer system extends the optical reach and analysis range of an existing Raman microspectrometer to allow analysis and/or repair of an oversized sample. The Raman microspectrometer system includes an extender for extending the optical reach of the existing microspectrometer and a supplemental stage which extends the analysis range of the existing microspectrometer by providing travel capabilities for non-destructive analysis of an entire oversized sample. Such an arrangement decreases manufacturing costs associated with testing oversized samples such as mammography panels, enabling analysis and/or repair to be performed without destruction.
Abstract: A laser microscope that efficiently performs 3D imaging irrespective of the shape of a specimen, includes an area segmenting section that segments an observation range of a specimen in the direction perpendicular to the optical axis of an objective lens into many areas; a surface-position storing section that stores motorized stage positions in association with the specimen surface positions, for the many areas; a surface-shape estimating section that estimates the specimen surface shape from the motorized stage positions and the specimen surface positions for the many areas; a z-scanning condition determining section that determines the specimen surface positions at desired positions of the motorized stage from the specimen surface shape; and a light detecting section that detects light from the specimen over certain ranges specified with reference to the specimen surface positions, in the optical axis direction of the objective lens.
Abstract: A tube lens unit for microscopes with achromatically corrective effect for the use with objectives with infinite image distance and achromatic residual errors. The tube lens unit includes of at least two lenses with the properties: nP<1.50 and vP<71 nN<1.66 and vN<37, wherein nP and nN signify the refractive index (ne) at a wavelength of 546 nm for a positive and negative lens each, and vP and vN signify the Abbe number (ve) at a wavelength of 546 nm for a positive and negative lens each, and the beam paths are characterized by the conditions: |?A|<0.60 and |?B|<0.30, wherein ?A signifies the variation of the aperture beam and ?B signifies the variation of the main beam while passing through the surfaces of the lenses.
Abstract: Methods and apparatus for reconstructing a wave, including interpolation and extrapolation of the phase and amplitude distributions, with application to imaging apparatus, such as microscopes.
Abstract: The present invention provides a plural third harmonic generation (THG) microscopic system and method. The system includes a laser device, a microscopic device, a beam splitter device and a photodetective device. By utilizing lasers with different central wavelengths or a broad band light source to simultaneously analyze THG response with respect to different wavelengths, a plurality of THG images and THG spectrum of the material or bio-tissue under stimulation of different wavelengths are obtained, thereby retrieving distributed microscopic images and resonant characteristics of the observational material or bio-molecules.
Abstract: A microscope system includes: a wavefront modulator that modulates a wavefront of light from a light source; an objective that irradiates a sample with light whose wavefront has been modulated by the wavefront modulator; a spherical aberration corrector that corrects spherical aberration caused by a difference between a refractive index of a medium between the objective and the sample and a refractive index of the sample; a refractive index calculator that calculates, for each wavelength of the light from the light source, an average refractive index of a medium between the objective and a condensing position of light emitted from the objective on the basis of an amount of the corrected spherical aberration; and a controller that controls the wavefront modulator to correct chromatic aberration calculated on the basis of the calculated average refractive index for each wavelength.
Abstract: An objective lens assembly 316 to be attached to a surgical microscope includes an objective lens 328, a roll section 318, a pitch section 320, and an attachment mechanism 330. The roll section 318 allows the objective lens 328 to be rotated sideways without causing a pair of eyepieces 324 to move. The pitch section 320 allows the objective lens 328 to be rotated towards and away from the pair of eyepieces 324 without causing the pair of eyepieces to move. A surgical microscope 300 including the objective lens assembly 316 also includes a microscope body 308 and support structure 302, 304, 306, and 310.
Abstract: The present invention relates to a system 100 for independently holding and manipulating one or more microscopic objects 158 and for targeting at least a part of the one or more microscopic objects within a trapping volume 102 with electromagnetic radiation 138. The system comprises trapping means for holding and manipulating the one or more microscopic objects and electromagnetic radiation targeting means (116). The light means comprising a light source and a spatial light modulator which serve to modify the light from the light source so as to enable specific illumination of at least a part of the one or more microscopic objects. The trapping means and the electromagnetic radiation targeting means (116) are enabled to function independently of each other, so that the trapped objects may be moved around without taking being dependent on which parts are being targeted and vice versa.
Abstract: Where a multimode fiber is used for light delivery in a microscope system and a transverse distribution of light exiting a distal end of the fiber is substantially uniform, the distal end is imaged onto a plane of a sample to be probed by the microscope system, or at a conjugate plane. Alternatively, the distal end is imaged onto a plane sufficiently close to the sample plane or the conjugate plane such that a radiant intensity of light at the sample plane or the conjugate plane is substantially uniform. In the case of a multi-focal confocal microscope system, the distal end of the multimode fiber is imaged onto a plane of a segmented focusing array. Alternatively the distal end is imaged onto a plane sufficiently close to the segmented focusing array plane such that a radiant intensity of the light at the segmented focusing array plane is substantially uniform.
Abstract: The present invention provides super-resolution optical imaging methods and systems, including: providing a sample to be optically imaged; providing a plurality of microstructures disposed substantially adjacent to a surface of the sample to be optically imaged; and providing a material disposed about the plurality of microstructures; wherein the plurality of microstructures have a first index of refraction; and wherein the material disposed about the plurality of microstructures has a second index of refraction that is substantially less than the first index of refraction of the plurality of microstructures. The plurality of microstructures include one of a plurality of microspheres and a plurality of microcylinders.
Type:
Application
Filed:
October 1, 2013
Publication date:
December 4, 2014
Applicant:
UNIVERSITY OF NORTH CAROLINA AT CHARLOTTE
Abstract: An immersion liquid for microscopy is provided, comprising (a) an organic compound which contains a saturated polycyclic hydrocarbon residue, (b) an oligomeric or polymeric saturated acyclic hydrocarbon and (c) an alkyl aromatic compound, selected from the group consisting of alkyl naphthalene and alkyl biphenyl.
Abstract: Disclosed herein is a stage system, including: a stage on which to mount a slide glass; a projection block projected more than the thickness of the slide glass to the side of that surface of the stage on which to dispose the slide glass; and a pressing block which is provided on that surface of the stage on which to dispose the slide glass, is thicker than the slide glass, and presses toward the projection block the slide glass disposed between itself and the projection block.
Abstract: An instrument and method for scanning at least a portion of a large specimen preferably causes the specimen to move relative to a two-dimensional detector array at a constant speed. The detector array takes one image of the specimen for each line that the detector moves. A controller controls a shutter of the detector array to open to take images and to pass the images to a processor, which is preferably a computer. The instrument takes one partial image of each part of the specimen that is being scanned and then combines those images with other images to produce a contiguous image.
Abstract: A system for performing sample probing. The system including an topography microscope configured to receive three-dimensional coordinates for a sample based on at least three fiducial marks; receive the sample mounted in a holder; and navigate to at least a location on the sample based on the at least three fiducial marks and the three-dimensional coordinates.
Type:
Grant
Filed:
November 18, 2013
Date of Patent:
November 25, 2014
Assignee:
DCG Systems, Inc.
Inventors:
Vladimir A. Ukraintsev, Richard Stallcup, Sergiy Pryadkin, Mike Berkmyre, John Sanders
Abstract: A method and apparatus for nanoscopy comprising a salt microlens. The microlens-based nanoscope comprises a conventional microscope, a microlens, and a XYZ piezoelectric stage is shown (SEE FIG. 1A). The microlens is mounted on a Z-stage and can be driven to accomplish the scanning. The specimen is placed above the microlens which is a plano-convex lens. The set up employed for the Salt Microlens for Ultra-high Resolution Imaging (SAMURI) can use a halogen-tungsten lamp with a dominant wavelength at 600 nm. A magnified virtual image of the specimen is obtained when the distance between microlens and specimen is less than the focal length of the microlens. The virtual image can then be magnified by the microscope and captured by eyes or a CCD camera.
Type:
Application
Filed:
March 12, 2014
Publication date:
November 13, 2014
Applicant:
BOARD OF TRUSTEES, SOUTHERN ILLINOIS UNIVERSITY
Abstract: Laser scanning microscope and method for the operation thereof having at least two detection channels which has at least one beamsplitter with a splitting of the sample light deviating from the 50:50 split and/or, with 50:50 split in the detection channels, has detectors with differently adjusted gain, or in at least one detection channel with equal light splitting has an additional light attenuator.
Abstract: A microscope system and observation control method. An electric zoom consecutively changes a magnification for a sample. A revolver includes a plurality of objective lenses of different magnifications and switches the objective lens placed in an observation optical path to intermittently change the magnification for the sample. A control section calculates a total magnification at which the sample is observed based on a magnification provided by the electric zoom and the magnification of the objective lens currently placed in the observation optical path. The control section operates when the revolver switches the objective lens placed in the observation optical path, to determine a target magnification that is the magnification of the electric zoom required to keep the total magnification obtained after the switching of the objective lens equal to the total magnification obtained before the switching of the objective lens.
Abstract: An ATR objective (1) for an IR microscope has a Cassegrain objective (2), an ATR crystal (7), a holding bar (8) to one end of which on the side of the sample, the ATR crystal (7) is mounted, a holding element (10), thin struts (9) which rigidly connect the holding bar (8) to the holding element (10) and intersect an optical path of the ATR objective (1) entering or exiting the Cassegrain objective (2) in such a fashion that they shade less than 10% of the beam cross-section of the optical path, and a motor drive (12) for axial movement of the holding element (10) relative to the sample position (3). The automated ATR objective thereby enables simple adjustment of operating modes and different contact pressures of the ATR crystal with respect to a sample (19).
Type:
Grant
Filed:
July 6, 2011
Date of Patent:
October 28, 2014
Assignee:
Bruker Optik GmbH
Inventors:
Michael Juette, Matthias Boese, Rolf Steinbrunn
Abstract: A microscope adapter unit disposed on an optical path of illumination light between a light source unit including a light source and a sample surface includes a first lens group having at least one lens and a second lens group having at least one lens. The first lens group converts the illumination light into roughly parallel luminous fluxes, and makes the illumination light enter the second lens group.
Abstract: An inverted microscope system includes an objective lens holding unit that holds an objective lens configured to collect at least observation light from a specimen, a tube lens configured to form an image using the observation light collected by the objective lens, a total internal reflection fluorescence microscopy optical system provided between the objective lens and the tube lens and configured to observe the observation light from the specimen using a total reflection illumination, and a disk scanning confocal optical system including a rotary disk on which a confocal opening is formed, the confocal opening being placed at a position substantially conjugate to a focus position of the objective lens. A relative distance between the focus position of the objective lens and the substantially conjugate position is changeable along an optical path of the observation light.
Abstract: An auxiliary arm attaching structure of a stand apparatus 1 includes a front member 3 that is attached to a support arm 2 of the stand apparatus and has a projection 6. The projection projects in a direction different from a longitudinal direction of the support arm and supports an auxiliary arm 8. Supported with the projection, the auxiliary arm is turnable so that an electronic image display unit 9 attached to a front end of the auxiliary arm is turnable to any position around a surgical microscope 4 supported with the front member.
Abstract: A compact lens system for imaging a sample comprising a substrate having a well formed therein. Index matching material is disposed in the well and a lens member is further disposed in the well in optical contact with the index matching material disposed in the well. A spacer member extends from at least one of the substrate and the lower transparent member to define a spacing from a focal point of the lens member, wherein the lens member and index matching material cooperate to image a sample disposed below the lower transparent member.
Type:
Application
Filed:
March 13, 2014
Publication date:
September 18, 2014
Applicant:
THE REGENTS OF THE UNIVERSITY OF MICHIGAN
Inventors:
Mayurachat Ning GULARI, Mostafa GHANNAD-REZAIE, Anurag TRIPATHI, Nikolaos CHRONIS
Abstract: Optical systems and apparatuses configured for enabling substantially simultaneous observation of a plurality of points in an array from a common reference point. Without the optical systems and apparatuses disclosed herein, less than all of the plurality of points can be observed substantially simultaneously from the common reference point.
Type:
Application
Filed:
March 15, 2013
Publication date:
September 18, 2014
Applicant:
BioFire Diagnostics, Inc.
Inventors:
Richard David Abbott, Patrick L. Riley, Zackery Kent Evans, Lyle M. Nay
Abstract: A transillumination device (150) for a microscope (100) comprises a flat panel light source (151), a diaphragm arrangement (152) arranged behind the flat panel light source (151) in the radiating direction (AR) that comprises two diaphragm elements movable relative to one another, at least one of the two diaphragm elements having a cutout, the two diaphragm elements defining, together with the at least one cutout, a diaphragm opening, wherein the dimensions of the diaphragm opening in two mutually perpendicular directions are determined by the position of the diaphragm elements relative to one another.
Type:
Grant
Filed:
January 26, 2012
Date of Patent:
September 16, 2014
Assignee:
Leica Microsystems (Schweiz) AG
Inventors:
Robert Paulus, Harald Schnitzler, Didier Henry
Abstract: A 4-Pi microscope for imaging a sample, comprising a first objective for focusing a first light beam on the sample at a spatial point one or more Digital Optical Phase Conjugation (DOPC) devices, wherein the DOPC devices include a sensor for detecting the first light beam that has been transmitted through the sample and inputted on the sensor; and a spatial light modulator (SLM) for outputting, in response to the first light beam detected by the sensor, a second light beam that is an optical phase conjugate of the first light beam; and a second objective positioned to transmit the first light beam to the sensor and focus the second light beam on the sample at the spatial point, so that the first light beam and the second light beam are counter-propagating and both focused to the spatial point.
Abstract: A microscope stand (11) is disclosed, including at least one pivot support (12), a mount (22) attached to a first end (15) of the pivot support (12), and a C-slide displacement assembly (112). The pivot support (12) is held to a stand interface (14) by a parallel guide mechanism (116), which allows the pivot support (12) to perform a circular motion in a vertical plane. The parallel guide mechanism (116) is formed by a cross-lever linkage which is rotatable about a cross-lever axis (115) extending centrally between and parallel to the support axis (113) of the pivot support (12) and the pivot axis (114) of the C-slide displacement assembly (112) and which is connected to both the stand interface (14) and the pivot support (12) in such a way that it transmits its own pivotal state simultaneously and equally to the stand interface (14) and to the pivot support (12).
Abstract: A correlation confocal microscope uses correlated photon pairs to improve resolution. It employs a source of a light beam converging to a point location on a sample, and an objective that gathers light from the point location and generates an image beam. A modulator applies a spatial pattern of modulation to the source light beam to define spatially correlated photons whose spatial correlations are preserved in modulated light gathered from the sample. A filter applies a modulation-selective filter function to the image light beam to generate a filtered light beam of like-modulated photons. A coincidence detector detects temporally coincident photon pairs in the filtered light beam, generating a pulse output that indicates the magnitude of a light-detectable property (such as transmissivity or reflectivity) of the sample at the point location. The modulator may apply phase modulation and the filter may be a phase-sensitive component such as an interferometer.
Type:
Grant
Filed:
June 27, 2012
Date of Patent:
September 9, 2014
Assignee:
Trustees of Boston University
Inventors:
David Simon, Alexander Sergienko, Lee Edwin Goldstein, Robert H. Webb
Abstract: The invention relates to an optical arrangement (20) and to a method of examining or processing an object (46). Here, a first laser pulse with a first central wavelength and a second laser pulse with a second central wavelength different from the first central wavelength are generated. Both pulses are superimposed in or on the object (46) such that multi-photon absorption takes place there with the involvement of at least one photon of the first laser pulse and at least one photon of the second laser pulse.
Type:
Grant
Filed:
December 21, 2012
Date of Patent:
August 26, 2014
Assignee:
Menlo Systems GmbH
Inventors:
Michael Mei, Ronald Holzwarth, Marc Fischer
Abstract: A light source and/or a deflector and/or the emitting end of an illuminating optical fiber is arranged in the rear focal plane of a lens for total internal reflection microscopy.
Type:
Grant
Filed:
May 1, 2006
Date of Patent:
August 26, 2014
Assignee:
Leica Microsystems CMS GmbH
Inventors:
Heinrich Ulrich, Werner Knebel, Kyra Moellmann
Abstract: In accordance with one embodiment of the present invention an apparatus for a low numerical aperture exclusion imaging apparatus is provided. The apparatus may include an electromagnetic illumination source for illuminating a portion of a specimen; and for collecting an image created by the electromagnetic radiation an objective lens optically coupled to the electromagnetic illuminated portion of the specimen. The apparatus also includes an optical blocking plate disposed between the objective lens and a focusing lens. The optical blocking plate is positioned to substantially block undesired electromagnetic radiation from image sources distally aligned in the same optical axis as the specimen. This invention is enhances narrow depth of field characteristics in imaging. It also enhances discreet imaging in a narrow focus field by eliminating some or most of the light which contributes to wide depth of field focus. This is useful for optical sectioning ranging from microscopy to photography.
Type:
Application
Filed:
April 27, 2014
Publication date:
August 21, 2014
Applicant:
The University of Vermont and State Agricultural College
Abstract: A microscope system includes: an objective; a correction apparatus which corrects a spherical aberration; a controller which obtains a plurality of combinations of a relative position of the objective to a sample and an optimum value, which is a set value of the correction apparatus in a state in which a spherical aberration caused in accordance with the relative position has been corrected, calculates a function expressing the relationship between the relative position and the optimum value on the basis of the obtained plurality of combinations by interpolation, and calculates the optimum value according to an observation target surface of the sample, on the basis of the function and the relative position which is determined from the observation target surface; and a correction apparatus driving apparatus which drives the correction apparatus in accordance with the optimum value, which is calculated by the controller.
Abstract: The invention relates to an illuminating device for a microscope with an illumination magazine comprising a plurality of light emitting units. A mechanical illuminator changer can change the light emitting unit currently active in the operative position. A filter magazine having a plurality of filter units is present, wherein a mechanical filter changer for changing the filter unit currently in the active operative position is associated with the filter magazine. At least one mechanical coupling component is provided for cooperation with the filter changer and the illuminator and for uniquely assigning each filter unit to a specific light emitting unit.
Type:
Grant
Filed:
March 24, 2009
Date of Patent:
August 19, 2014
Assignee:
Carl Zeiss MicroImaging GmbH
Inventors:
Leander Dietzsch, Christian Boeker, Andreas Nolte, Thomas Belkner, Michael Wagener
Abstract: A microscope system for imaging of an object that can be placed in an object plane of a microscope system includes an imaging system for providing at least one pair of optical imaging paths that include a stereoscopic angle in the object plane. The imaging system includes a first subsystem comprising a first plurality of optical lenses that are commonly traversed by both optical imaging paths of the at least one pair of optical imaging paths. The imaging system also includes a second subsystem comprising a second plurality of optical lenses that are traversed by only one optical imaging path. At least two optical lenses of the first plurality of optical lenses and at least two optical lenses of the second plurality of optical lenses are displaceable relative to one another along a common optical imaging path to respectively vary a magnification of the representation of the object.
Abstract: A microscope adapter unit disposed on an optical path of illumination light between a light source unit including a light source and a sample surface includes a first lens group having at least one lens and a second lens group having at least one lens. The first lens group converts the illumination light into roughly parallel luminous fluxes, and makes the illumination light enter the second lens group.
Abstract: A method for the optical detection of an illuminated specimen, wherein the illuminating light impinges in a spatially structured manner in at least one plane on the specimen and several images of the specimen are acquired by a detector in different positions of the structure on the specimen. An optical sectional image and/or an image with enhanced resolution is then calculated. The method includes generating a diffraction pattern in the direction of the specimen in or near the pupil of the objective lens or in a plane conjugate to the pupil. A phase plate with regions of varying phase delays is dedicated to the diffraction pattern in or near the pupil of the objective lens or in a plane conjugate to said pupil, and different phase angles of the illuminating light are set.
Type:
Grant
Filed:
August 14, 2013
Date of Patent:
August 5, 2014
Assignee:
Carl Zeiss MicroImaging GmbH
Inventors:
Michael Schwertner, Ralf Wolleschensky, Michael Kempe
Abstract: A device for holding filters for a microscope includes a filter wheel (20) rotatable about an axis of rotation (16) and a drive unit (14) for rotating the filter wheel (20). The filter wheel (20) comprises a basic body (44) rotatable about the axis of rotation (16) and at least one segment (46-54) selectively connectable to the basic body. The segment (46-54) comprises at least two housing areas (58, 59) each holding at least one filter (11). Another housing area may provide a transmission range (36, 59) for unfiltered transmission of light. A second rotatable filter wheel may be arranged at a location displaced along the axis of rotation relative to the filter wheel, and may have its own respective transmission range for unfiltered transmission of light, whereby a filter on either filter wheel may be aligned with the transmission range on the other filter wheel.
Abstract: The surgical microscope system includes a first binocular microscope and a first display device. The first binocular microscope includes an objective lens, a first right ocular lens which provides a first image based on a light flux transmitted through the objective lens, and a first left ocular lens which provides a second image based on a light flux transmitted through the objective lens. The first display device can be disposed opposite to or in alignment with the first binocular microscope, and includes a first right-eye image display surface for displaying the first image and a first left-eye image display surface for displaying the second image. The first display device can be reversed about a horizontal axis extending in a direction along which the first right-eye image display surface and the first left-eye image display surface are located in alignment.