Abstract: Provided herein are devices and systems that apply full-field optical coherence tomography (OCT) technology to three-dimensional skin tissue imaging. A special designed Mirau type objective and an optical microscope module allowing both OCT mode and orthogonal polarization spectral imaging (OPSI) mode are disclosed.

Abstract: A method for SPIM microscopy, wherein the sample is moved continuously, and a plurality of images are taken at time intervals by means of a detection arrangement during the movement. The image capture duration or exposure time is dimensioned such that the movement path of the sample lies within a predetermined resolution range of the detection objective. The speed of the sample movement is determined and set by the image capture duration or exposure time and/or the distortion of the point spread function generated by the sample movement of the sample. The image blur is corrected computationally by the respective image capture duration and the movement speed. A sharp image is generated in this way. The actual optical section thickness of the light sheet is determined from the light sheet thickness, and the movement speed is determined therefrom and from user settings.

Abstract: An imager system and a display system for a vehicle are provided. The imager system includes at least one imager configured to capture a multi-focus image having depth data. The display system includes at least one display assembly configured to display a multi-focus image having depth data.

Abstract: Platelets or blood cells are detected in a fluid sample by adjusting a focal depth of a microscope through a range of values, the microscope having a mounted sample and an objective lens adapted with one or both of (a) a spherical aberration correction unmatched to a utilized cover plate for the sample, or (2) a numerical aperture unmatched to a utilized illumination source for the sample. Images are recorded at different specific focal depths and in multiple z planes of a fluid bearing the platelets, where the position of platelets may overlap on different of the multiple z planes that are recorded, the images recorded through the cover plate, thus causing the generation of a specific light-dark pattern indicative of platelets at particular positions and at multiple depths in the fluid media. The images are analyzed for the specific light-dark pattern.

Abstract: An analyzer is disclosed for optical analysis of a biological specimen. In at least one embodiment, the analyzer includes optics which includes a camera, intermediate optics and front optics. The intermediate optics is movably arranged and the front optics is fixedly arranged. An analyzer for optical analysis of a biological specimen, in at least one embodiment includes a robot for transporting a slide to be analyzed. The robot is controlled by at least three motors to allow movement of the robot in three dimensions. The robot includes a handling device configured to grip the slide.

Abstract: A focusing apparatus for use with an optical system having a high NA objective lens includes an image forming and capturing mechanism for forming an image in an intermediate image zone and for capturing an image by receiving and refocusing light from a selected focal plane within the intermediate image zone, and a focus adjusting mechanism for adjusting the position of the selected focal plane within the intermediate image zone. The image forming and capturing mechanism includes at least one high NA lens. In use, spherical aberration introduced by the high NA objective lens is reduced.

Abstract: A light blocking member (18) for application, to a microscope is provided. Although it is producible at a sufficiently low cost, it can sufficiently block ambient light, and does not require a special operation when a sample (16) is placed on a stage (2) or the sample is taken out from above the stage. The light blocking member (18) is formed from a soft polymer, has an inner peripheral surface which is cylindrical in at least an upper part thereof, and is held on an objective lens assembly by fitting the upper part onto the outer peripheral surface of the objective lens assembly.

Abstract: An illumination device for an optical viewing apparatus has a light source including a first individual light source and a second individual light source arranged in a plane. The first individual light source has a first midpoint and the second individual light source has a second midpoint. An axial direction is defined by a vector from the second midpoint to the first midpoint. An illumination optical unit defines an optical axis which is arranged perpendicularly to the plane and intersects the plane at an intersection point. The light source is imaged toward infinity by the illumination optical unit. The first individual light source has an extent along the axial direction. The midpoint is offset by an amount (?) in a positive direction along the axial direction relative to the intersection. The relationship 0.1*L1???1*L1 is satisfied for the offset.

Abstract: Surgical observation system for observing, in three dimensions, a surgical site from two directions are provided herein. The surgical observation system may include a first image-acquisition optical system that obtains, from a first observation direction, a pair of optical images, having parallax, of a subject via a pair of apertures disposed side-by-side; and a second image-acquisition optical system that obtains, from a second observation direction in which an angle about an optical axis differs from that of the first observation direction, another pair of optical images, having parallax, of a subject via another pair of apertures disposed side-by-side.

Abstract: The invention relates to a method for operating a microscope device having a first and a second zoom microscope, wherein a zoom adjustment of the second zoom microscope is carried out in dependence upon a zoom adjustment of the first zoom microscope.

Abstract: An imager system configured for a vehicle control system is provided that includes at least one imager configured to capture a multi-focus image having depth data, the imager comprising an image sensor comprising an array of pixels, and an optics assembly in optical communication with the image sensor, the optics assembly configured to capture light rays, wherein the optics assembly comprising a main lens assembly configured to substantially focus a scene on to a plane, a micro lens assembly in optical communication between the main lens and the image sensor to substantially focus on to same the plane as the main lens assembly extending out to infinity, and a controller in communication with the imager, wherein the controller is configured to output a signal for controlling a function of the vehicle, the signal based upon the depth data determined from the multi-focus image.

Abstract: An optical fiber rotary squeezer polarization controller, comprising a base, a left bracket and a right bracket respectively mounted at both ends of the base, characterized in that a shaft is rotatably mounted between the left bracket and the right bracket, the shaft having a through channel cut therein lengthwise for placement of an optical fiber, the shaft further being mounted with a squeezing apparatus corresponding to the channel. The present invention has altered the means by which an optical fiber is fixed onto the prior-art polarization controllers and has eliminated the clamps at both ends of the optical fiber. The shaft carries the squeezing apparatus to rotate to any desired angles while the optical fiber remains stationary under the effect of its own tension. As the optical fiber extends outwardly from either end of the shaft, it is restrained by its own tension and, therefore, will not rotate along with the shaft.

Abstract: Illumination system for a microscope system capable of being mode-switchable between a first and a second illumination mode, comprising one source of light for providing a collimated beam of light, at least one selector mirror capable of being positioned in at least two positions to redirect the beam of light in two different beam paths, the first beam path being a direct exit beam path wherein the selector mirror redirects the beam of light along an exit beam path to provide a first illumination mode, the second beam path is a mirror loop path comprising two or more mirrors arranged to redirect the beam of light onto the selector mirror such that it is redirected by the selector mirror a second time along the exit beam path, and wherein mirror loop path comprises at least one optical element arranged to optically alter the beam of light to provide the second illumination mode.

Abstract: A projector for projecting an image includes a light source that outputs light; a reflecting member that reflects the light from the light source; and a diaphragm member with an aperture that restricts a range of flux of either an incident light incident to the reflecting member or a reflected light reflected by the reflecting member. The aperture may penetrate through the diaphragm member along a direction of thickness of the diaphragm member. The diaphragm member may be arranged so as to not block the other of the incident light or the reflected light, and a direction perpendicular to the direction of thickness may be oblique with respect to an optical path of either the incident light or the reflected light.

Abstract: An immersion microscope objective includes, in order from an object side, a first lens group having a positive refractive power, a second lens group, and a third lens group, wherein the first lens group changes a light beam from an object to a convergent light beam, the second lens group has a refractive power smaller than that of the first lens group, and the following conditional expression (1-1) is satisfied: 7.5 mm<NA0×d0 (1-1) where NA0 denotes an object-side numerical aperture of the immersion microscope objective, and d0 denotes a working distance of the immersion microscope objective.

Abstract: A biological specimen observation apparatus whereby observation of a biological specimen can be performed accurately. In macro observation, a biological change region is extracted from a macro image, a micro observation point corresponding to an extracted biological change region is registered, and an object for tracking is identified. In micro observation, it is judged from the micro image whether or not biological change has continued in the biological change region at the micro observation point, and the registered micro observation point is updated on the basis of this judgment result. It is possible to carry out both macro observation for detecting a biological change region and micro observation for observing the progress of growth of a partial minute region where biological change has been exhibited, and to carry out accurate observation of a biological specimen.

Abstract: A dual-configuration microscope is provided. The microscope may be converted into an upright configuration or an inverted configuration. The microscope includes a base. The microscope further includes a body having a first portion and a second portion, wherein the body is rotatably coupled to the base at a rotational coupling. The microscope further includes one or more objectives coupled to the first portion of the body, a condenser coupled to the second portion of the body and a stage positioned between the one or more objectives and the condenser. The stage includes a first specimen supporting surface and a second specimen supporting surface, wherein the second specimen supporting surface is positioned opposite the first specimen supporting surface.

Abstract: An aberration correction optical unit (3) disposed in an optical system includes: a first phase modulation element (3a) and a second phase modulation element (3c) each having a polarization characteristic; and a variable waveplate (3b) disposed between the first and second phase modulation elements so that an optical axis of the variable waveplate has a predetermined angle with respect to optical axes of the two phase modulation elements, in order to correct an aberration generated by the optical system.

Abstract: A microscope system includes: an objective; a correction apparatus which corrects a spherical aberration; a controller which obtains a plurality of combinations of a relative position of the objective to a sample and an optimum value, which is a set value of the correction apparatus in a state in which a spherical aberration caused in accordance with the relative position has been corrected, calculates a function expressing the relationship between the relative position and the optimum value on the basis of the obtained plurality of combinations by interpolation, and calculates the optimum value according to an observation target surface of the sample, on the basis of the function and the relative position which is determined from the observation target surface; and a correction apparatus driving apparatus which drives the correction apparatus in accordance with the optimum value, which is calculated by the controller.

Abstract: A microscope is provided. The microscope includes: a detection section for detecting the measurement light; a first image acquisition section emitting the visible light onto a detection surface to obtain an optical image; and a switch mirror or beam splitter disposed on a light path, along which the measurement light from the analysis position of the sample is guided to the detection section. The microscope further includes a second image acquisition section that is disposed in a position apart from the light path of the detection section for obtaining an optical image of a large area which includes the analysis position of the sample, wherein the optical image of the large area is larger than an optical image of an area, which includes the analysis position of the sample, obtained by the first image acquisition section.

Abstract: A scanning probe microscopes including an imaging device (optical microscope) which images the cantilever, a device is provided which estimates the resonance frequency of the cantilever from the cantilever image imaged by the imaging device, as a result of which, even when information on the cantilever is unknown, the cantilever is actually excited to perform measurement of resonance frequency within a specified frequency range centered on the estimated resonance frequency, thereby enabling measurement of resonance frequency within an appropriate frequency range and making it possible to avoid obtaining an incorrect resonance frequency and to eliminate the waste of performing resonance frequency measurements while changing the frequency range settings in trial-and-error fashion.

Abstract: A sequence of individual images is acquired by imaging the sample through imaging optics onto an image sensor. For the acquisition of each individual image, the sample is provided with a marker pattern, in which individual markers can be imaged in the form of spatially separable light distributions through the imaging onto the image sensor. The centroid positions of the light distributions are determined and superimposed to form a complete image of the sample. According to the present invention, an image-drift-inducing temperature value (?T1, ?T2, . . . , ?Tn) is measured during the acquisition of the sequence of individual images. A temperature-dependent drift value (?X1, ?X2, . . . , ?Xn; ?Y1, ?Y2, . . . , ?Yn) is correlated to the image-drift-inducing temperature value (?T1, ?T2, . . . , ?Tn) based on predetermined correlation data. The determined centroid positions are corrected based on the drift value (?X1, ?X2, . . . , ?Xn; ?Y1, ?Y2, . . . , ?Yn).

Abstract: A microscope system includes a microscope frame on which a stage is placed; and a camera head, in which an objective lens is attachable, for capturing an image of a specimen, the camera head being attached to the microscope frame by being fitted, and being slidable with respect to the microscope frame in a direction that is parallel with a surface of the stage placed on the microscope frame and orthogonal to an optical axis of the attached objective lens.

Abstract: A laser scanning microscope (LSM) consisting of at least one light source from which an illumination beam path extends in the direction of a sample, at least one detection beam path for transmitting sample light to a detector array, a first pinhole for confocal filtering in front of the detector array, a scanner for causing a relative motion between the illumination light and the sample in at least one direction, and a microscope lens. For illuminating a sample, at least two illumination beams, which the microscope lens focuses as illumination points in a sample plane, are generated in the illumination beam path. The laser scanning microscope is characterized in that in addition to the preferably adjustable, slit-shaped first pinhole, a second, preferably adjustable, slit-shaped pinhole is arranged downstream of the first pinhole so as to create optically conjugate beams arranged between the first and the second pinhole.

Abstract: A microscope system includes: a main body portion having an optical system for forming an image from at least observation light from a specimen; an objective lens unit having an objective lens for taking in the observation light and having a base portion with an approximately belt shape for holding the objective lens on one end; and a unit attachment portion that is held by the main body portion and is configured to detachably attach the base portion at a position where at least an optical axis of the objective lens coincides with an optical path of the observation light.

Abstract: Provided is a magnification observation device in which a focus of the imaging unit can be focused on the observation surface of the object in observation object region in a short period of time. An unit region of an observation object is imaged at a plurality of Z-positions by moving a object lens in the light-axis direction, the plurality of pieces of image data corresponding to the unit region are captured. An image of the observation object is displayed in the display unit as a navigation image. Shape data which indicates the position of the surface of the observation object in the unit region is generated. An observation object region on observation object is designated based on the navigation image. Based on the generated shape data, a focus of the object lens is on the surface of the observation object on the observation object region designated.

Abstract: The present invention relates to a method for illuminating a sample (10) in a microscope (26), the sample (10) being analysed in transmitted light bright field illumination or in incident light fluorescence illumination, wherein a white light LED (4) is used as the light source for the transmitted light bright field illumination, and a shutter (6) is activated at a location in the illumination beam path of the transmitted light bright field illumination during incident light fluorescence illumination and this shutter (6) is deactivated during transmitted light bright field illumination.

Abstract: A dual-configuration microscope is provided. In some aspects, the microscope can be converted into an upright configuration or an inverted configuration. The microscope includes a base having a lower portion and an upper portion, the lower portion configured to support the microscope. The microscope further includes a body having a first portion, a second portion, and an intermediate portion extending between the first and second portions. The body is rotatably coupled to the base at a rotational coupling that defines a rotating axis that extends in a longitudinal direction with respect to the microscope.

Abstract: An optical system for eye tracking is disclosed. The system includes a light guiding prism that guides light from an ocular object to an imaging system through multiple internal reflections. The light guiding prism may include one or more freeform surfaces having optical power.

Abstract: The invention relates to a method for calibrating an optical instrument which comprises at least a motorized zoom system, an objective, an image sensor and an image processing unit. The method comprises the following steps: establishing calibration data DZRef of the zoom system with a reference objective and storing these in an internal memory of the zoom system; establishing calibration data DORef of the objective with a reference zoom system and storing these in an internal memory of the objective; reading the internal memories of the zoom system and of the objective and applying a digital-optical correction of an image acquired by an image sensor with the calibration data DZRef and DORef. The invention moreover relates to an optical instrument, in particular a digital microscope, to which the calibration method according to the invention can be applied.

Abstract: A super-resolution microscope comprises: an illumination optical system that condenses a first illumination light beam for exciting the molecule from a stable state to a first quantum state and a second illumination light beam for further transitioning the molecule onto a sample in a manner that the first and the second illumination light beams are partially overlapped; a scanning section that scans the sample by relatively displacing the first and the second illumination light beams and the sample; a detection section that detects an optical response signal emitted from the sample; and a phase plate that is arranged in the illumination optical system and has M surface areas for modulating the phase of the second illumination light beam, wherein the phase plate comprises a monolayer optical thin film with M surface areas formed on an optical substrate with a thickness that satisfies the predetermined conditional expression.

Abstract: A confocal microscope includes an illumination optical system configured to uniformly illuminate at least part of a first confocal stop with a light beam from a light source, a first collection optical system configured to collect a light beam passing through the first confocal stop onto a specimen, a second collection optical system configured to collect a light beam from the specimen onto a second confocal stop, a detection unit configured to detect a light beam passing through the second confocal stop, and a light intensity control member provided to at least one of the first collection optical system and the second collection optical system and having a transmittance of a first region including an optical axis that is lower than a transmittance of a second region around the first region.

Abstract: Standing wave-generating planar wave illumination units generate two light-wave planar waves using light from a light source assembly and irradiate a sample surface with a standing wave produced by interference of the planar waves on the sample surface. A bias planar wave illumination unit generates a bias planar wave using light from the light source assembly and irradiates the sample surface with the wave. The bias planar wave is synchronized with the standing wave to alternately oscillate to positive and negative with an equal electric field displacement irrespective of the position on the sample surface across a reference time specified in advance when the displacement of the standing wave has a value “0” at the respective positions on the sample surface, and oscillates at a bias amplitude specified in advance to raise the displacement of the standing wave on the sample surface to only a positive or negative displacement.

Abstract: Spatial frequency swept interference (SFSI) illumination and imaging methods and devices that interfere two collimated coherent beams to generate an interference pattern of a plurality of illuminating sheets with sweeping spatial frequency.

Abstract: The invention concerns a scanning device for focusing a beam of rays in defined regions of a defined volume, comprising an input optics wherein the beam of rays penetrates first, having at least one first optical element; a focusing optics for focusing the beam of rays exiting from the input optics; and a deflecting device arranged between the first optical element and the focusing optics, for deflecting the beam of rays after it has passed through the first optical element, based on a position of the focus to be adjusted in lateral direction. In order to adjust the position of the focus of the beam of rays in the direction of the beam of rays, and optical element of the input optics can be displaced relative to the deflecting device.

Abstract: A microscope apparatus includes a monitoring optical system, an imaging unit capturing an image of an observation target through the monitoring optical system to generate a plurality of images, a correction unit disposed in the optical monitoring system and correcting various aberrations which occur due to an observation condition, and a decision unit deciding a correction amount of the correction unit based on the plurality of images generated by the imaging unit, whereby an image deterioration ascribable to the aberration occurring due to the observation condition in the microscope apparatus is appropriately and easily corrected according to a use condition of the microscope apparatus.

Abstract: A widefield microscope illumination system and a method for illumination, the system having a microscope objective with an optical objective axis, an illumination light source sending widefield illumination light along illumination beam paths having corresponding illumination axes along which the illumination light penetrates into the microscope objective through illumination light entry sites located within a predetermined illumination light entry area, a spatially resolving light detector detecting detected light sent from an illuminated sample through the microscope objective along a detected light beam path, and an automatic illumination light beam path manipulation device, controlled by a control system, which is arranged in front of the microscope objective in relation to the direction of the illumination light beam path, and by which illumination light beam path manipulation device the illumination axes are automatically movable at time intervals to a plurality of illumination light entry sites.

Abstract: A broad-spectrum, multiple wavelength illuminator comprises a luminescent body, and a plurality of semiconductor chips spaced apart from the luminescent body emitting light within one or more wavelength ranges towards the luminescent body, causing the luminescent body to emit light of one or more wavelength ranges. An optical element adjacent to the luminescent body collects light emitted by the luminescent body. An optical device collects light collected by the optical element. An aperture located between the optical element and the optical device passes the light emitted by the luminescent body along an optical axis, wherein light collected by the optical element and the optical device and passed by the aperture forms a beam of light illuminating a target. Alternatively, instead of being spaced apart from the chips, the luminescent body may be a layer adjacent to the chips.

Abstract: An imaging apparatus includes: a first imaging area setting unit configured to divide an imaging range that includes a sample into a plurality of areas and set each of the plurality of areas as a first imaging area; a first imaging unit configured to capture a first image at an in-focus position of the first imaging area; a second imaging area setting unit configured to set an area that extends over adjacent first imaging areas as a second imaging area in a case where in-focus positions of the adjacent first imaging areas are so different from each other that the difference exceeds a predetermined value; a second imaging unit configured to capture a second image at an in-focus position of the second imaging area; and an image combining unit configured to combine the first image with the second image.

Abstract: An example apparatus may include a light source for illuminating a sample, an objective lens positioned on a light path extending from the sample, a lenslet array having a plurality of lenslets and positioned along the light path to receive light from the objective lens. The lenslet array may be positioned along the light path at substantially a Fourier plane of the sample. The example apparatus may also include a detector positioned along the light path approximately one lenslet focal length from the lenslet array. The plurality of lenslets of the lenslet array may correspond to portions of the detector. Each lenslet of the lenslet array may transmit to a corresponding portion of the detector an image of the same portion of the sample from a different viewing angle.

Abstract: A detection device (1) includes a light receiving unit (13a) which receives fluorescence; a light receiving intensity calculation unit (32) which calculates light receiving intensity of fluorescence which is received by the light receiving unit (13a) while changing a relative position (L) between the light receiving unit (13a) and a test subject (100); and a specifying unit (33) which specifies an optimal position of the relative position (L) where the light receiving intensity which is calculated by the light receiving intensity calculation unit (32) becomes a maximum, and in which fluorescence is detected using the optimal position which is specified by the specifying unit (33).

Abstract: A wide-field optical microscope and method capable of resolving images down to 0.1 {acute over (?)} with a magnification range in excess of 250 million power includes an objective having a primary and a secondary element. A sample is held so that the area of interest is at a location that is closer to the primary element than the focal length of the primary element. The primary element collects and collimates light reflected from the sample. The secondary element then focuses the collimated light onto a pinhole aperture, which blocks all light rays that were not parallel, thus producing a non-focused reflected pattern. The non-focused reflected pattern passes through a field stop and is then magnified by one or more negative optical elements and additional field stops to produce an enlarged pattern.

Abstract: A microscope system including a stage on which a specimen is mounted and that can be moved in a direction that intersects with an optical axis of illumination light irradiated on the specimen; an observation optical system that acquires an image of the specimen on which the illumination light is irradiated; a viewing-range setting unit that sets a viewing range of the image acquired by the observation optical system and displayed on a display unit; a ratio calculating unit that calculates a ratio of the viewing range of the image, which is set by the viewing-range setting unit, relative to a maximum image-acquisition area that can be captured by the observation optical system; and a stage controller that controls a moving speed of the stage in accordance with the ratio calculated by the ratio calculating unit.

Abstract: A stereoscopic image capturing system includes a plurality of lens devices with optical elements, a vibration detection unit, a control unit that calculates a drive signal to drive the optical element(s) for correcting image blurring based on an output from the vibration detection unit, and a driving unit that drives the optical element(s) based on the drive signal.

Abstract: The present invention relates to a method and optical device for Raman spectroscopy and for observing a sample, said device including an optical means for superimposing an excitation laser beam having a spectral band B0 and an observation beam having a spectral band BV so as to form a combined excitation and observation incident beam, and an optical separation means arranged in the path of a collected beam coming from scattering on the sample and including a first filtering means, a second filtering means capable of spatially separating said collected beam into a first secondary beam and two tertiary beams, each of which includes a spectral band selected from the spectral band B0 of the laser, the spectral band BV of the observation beam, and the spectral band BR of the Raman scattering beam, respectively.

Abstract: A pinhole changing device for a confocal microscope is detailed herein. Several pinholes of different sizes are contained on a single disk. A precision XY stage is used to switch between the several pinholes. The same device could be used as a spatial filter for a multi-photon microscope. One can also add beam expansion control and spherical aberration correction to the same device with no additional loss.

Abstract: Provided is a nonlinear optical device capable of alleviating, without the need for a complicated compensation mechanism, temporal broadening and the waveform distortion resulting from a group-velocity dispersion slope, to thereby irradiate an object with short optical pulses having high peak power. The nonlinear optical device includes a short optical pulse source (10) for generating short optical pulses and a short optical pulse delivery system (20) for delivering the short optical pulses generated from the short optical pulse source to an object, in which there is generated substantially no nonlinear optical effect and there is substantially no amount of group-velocity dispersion, the short optical pulse source generates short optical pulses, and the short optical pulses have a spectral width (full width at half maximum) ?FWHM satisfying ?1<?FWHM<?2.

Abstract: An apparatus for transmitted light illumination for light microscopes A diaphragm edge may be variably positioned in the direction of the optical axis, wherein a position of the diaphragm edge in the direction of the optical axis can be varied irrespectively of a position of the diaphragm edge transversely to the optical axis. A separate sample support table may be mounted on a housing. The housing has a passage opening, through which the diaphragm edge can be moved in the direction of the optical axis. A holding device is formed in the region of the passage opening of the housing or on a separate sample support table and a control device is present which is adapted to position the diaphragm edge in dependence at least upon a determined presence of a sample support table.

Abstract: A microendoscope, and a microendoscopy method related to the microendoscope, each include a tube housing, where an end of the tube housing is shaped and finished to facilitate collection of light emitted from a sample when examined using the microendoscope. In addition, a catadioptric lens assembly, an endomicroscope that includes the catadioptric lens assembly and a microendoscopy method for microscopic analysis that uses the endomicroscope are predicated upon a second element and a third element within the catadioptric lens assembly that each has a dichroic coating. The placement of the dichroic coating on the second element and the third element provides for different magnification factors as a function of illumination wavelength when using the microendoscopy method.

Abstract: An observation system includes: a container holding unit; a ring illumination, including a light source in a ring shape, arranged in a position opposed to an outer bottom surface of a container so that a central axis of the illumination is aligned to that of a bottom surface of the container held by the container holding unit, when an observation target is observed; a first light-shielding plate in a ring shape, having an inner diameter capable of varying, arranged between the illumination and the container holding unit, and configured to shield light from the illumination; a lens, arranged in a position opposed to an inner bottom surface of the container held by the container holding unit, to observe the observation target; and a second light-shielding plate, having an outer diameter capable of varying, arranged in an internal space of the illumination, and configured to shield light from the illumination.