Abstract: A method of replacing an ion source in a mass spectrometer (MS) system is provided, where the ion source includes an ionization volume, at least one ionizing element and at least one focusing element, and where the mass MS system includes the ion source, a vacuum chamber that houses the ion source, and an interlock chamber. The method includes opening a valve between the interlock chamber and the vacuum chamber, moving the ion source into the interlock chamber through the opened valve and closing the valve, and removing the ion source from the interlock chamber. The ion source may further include means for plugging into a docking station in substantially one action, where the docking station provides sufficient electrical connection, upon plugging with the ion source, for operation of the ion source.

Abstract: A mass analyzer comprises a pair of planar electrode structures. The electrode structures are disposed opposite one another, parallel to one another, and axially offset from one another. One of the pair of planar electrodes comprises an opening. The mass analyzer comprises an ion mirror disposed between the pair of planar electrodes. A mass spectrometer and a mass spectrometry method are also described.

Abstract: Methods, systems and computer readable media for controlling an instrument in communication with a host computer are provided. Operations of an instrument that must be completed on schedule are controlled via an embedded controller embedded in the instrument. A complete status packet is sent to a host computer from the embedded controller. Periodically, the embedded controller repeats the sending of a complete status packet to the host computer, wherein status values in the complete status packet are updated with each iteration of sending a complete status packet.

Abstract: In an example embodiment, the system receives user inputs including: (1) a netlist that describes the circuit elements and connections of an integrated circuit; (2) a mathematical device model that corresponds to the integrated circuit and that includes an equation with a number of parameters and decision variables grouped into terms; and (3) a description of an input voltage. Based on these inputs, the system calculates an output such as a voltage or current. The system displays a GUI that includes a view which shows each of the terms of the equation after a value has been assigned to each parameter and to each decision variable and a view which shows each of the terms of the equation after reduction of the values assigned to each parameter and each decision variable. Upon receipt of a user's change to the value of a parameter, the system promptly updates the views.

Abstract: An interface for use in a mass spectrometer is disclosed. The interface comprises a first ion funnel comprising a first inlet and a first outlet, and a first axis between the first inlet and the first outlet. The interface further comprises a second ion funnel in tandem with the first ion funnel, the second ion funnel comprising a second inlet and a second outlet, and a second axis between the second inlet and the second outlet. The first axis and the second axis are offset relative to one another. A mass spectrometer comprising the interface and a method are disclosed.

Abstract: A system includes at least two IEEE 1588 nodes. The nodes are connected by two paths: a low latency communication path and a high bandwidth path. The clocks within the nodes are synchronized to one another at a network interface. Data and timing are transmitted on the high bandwidth path while critical timing signals are passed on the low latency communication path thereby improving timing synchronization.

Abstract: A plunger for a syringe used to inject gas and liquid chromatography samples has an end portion surrounded by a flexible sleeve. The sleeve forms a seal in the bore of a barrel traversed by the plunger, the bore being in fluid communication with a cannula attached to the end of the barrel. The plunger has an end surface that is exposed and is flush with an annular end surface of the sleeve. The plunger end surface, and not the sleeve, contacts the cannula upon injection and avoids imposing cyclical hoop stresses on the barrel which tend to crack the barrel after a finite number of cycles. In a further embodiment, a thin layer of sleeve material covers the end surface of the plunger. The thin layer avoids inducing significant barrel stress.

Abstract: In some embodiments, a nuclear magnetic resonance (NMR) receiver using digital downconversion and subsampling tracks transmit and/or receive signal phases according to time(s) elapsed since reference times (e.g. reset times) corresponding to known phases. Carrier-frequency (fc) NMR signals are digitized directly, using a sampling frequency (fs) lower than fc. Digital downconversion and carrier phase correction are performed to yield NMR modulations of interest. A phase determination circuit may include an N-bit counter incremented at fs and an associated count register storing an indicator of a time elapsed since a carrier generator reset for each transient sampling period start, A real-time controller synchronously resets the counters and corresponding signal generators. A sin/cos lookup table maps counts to phase correction function values.

Abstract: A method for optimizing loop gain of an atomic force microscope (AFM) apparatus includes determining a change in gain of the physical system and adjusting a controller frequency response of the controller in an AFM loop to compensate for the determined change in gain. The AFM loop has a corresponding loop response that includes the product of the controller frequency response and a physical system response of the physical system.

Abstract: Methods, systems, and computer readable media for determining the quality of a CGH array, including calculating a spread of the derivative of log ratio value differences between consecutive probes representing consecutive positions along a chromosome, wherein ratio values are calculated from probe signals from a CGH array.

Abstract: A system for measuring inter-carrier interference (ICI) in an OFDM system includes a test symbol generator coupled to a transmitter of the OFDM system to generate an N*N orthogonal matrix having N*N test symbols, and to send the test symbols in a test symbol stream via the transmitter to a receiver of the OFDM system. The N*N test symbols are arranged by the test symbol generator into an N*N orthogonal matrix before being sent to the receiver. The system also includes an ICI measuring module coupled to the receiver to detect the N*N test symbols received in the receiver and to arrange the test symbols as a receiving matrix in the same way as the orthogonal matrix in the transmitter. The ICI measuring module outputs the receiving matrix as an ICI matrix of the OFDM system, wherein an element on the kth row, lth column of the ICI matrix represents interference from the lth sub-carrier on the kth sub-carrier, wherein k?l.

Abstract: A network analyzer comprises: a signal source configured to supply an input signal to a device, wherein the device is configured to generate a phase reference signal; a receiver configured to receive the phase reference signal from the device and to measure a phase response of the device according to the phase reference signal; and a calibration component configured to compare the measured phase response of the device with an actual phase response of the device to identify a tracking parameter for the receiver.

Abstract: A thermal conductivity detector includes a structure defining a cavity, the structure principally comprising a material having a first coefficient of thermal expansion; a sensing element for sensing a thermal conductivity of a gas flowing within the cavity, the sensing element having a second coefficient of thermal expansion different from the third first coefficient of thermal expansion, the sensing element being disposed at least in part within the cavity; and a compensation structure having a third coefficient of thermal expansion different from the first and second thermal coefficients of expansion. Over a selected temperature range, a stress within the sensing element is less than a yield stress of any component of the sensing element, and a stress within the compensation structure is less than a yield stress of any component of the compensation structure.

Abstract: The present invention provides methods, nucleic acids, compositions, and kits for detecting microRNA (miRNA) in samples. The methods comprise designing mRNA-specific primers, adding a polyA tail to the miRNA, and using reverse transcription and amplification to detect the miRNA. The nucleic acids, compositions, and kits typically comprise some or all of the components necessary to practice the methods of the invention.

Abstract: A method and apparatus generate a measured data set by: (i) providing a probe tip at a selected height from a doped region of a substrate, (ii) applying a probing signal to the probe tip, (iii) measuring a characteristic of an electrical interaction between the probe tip and the doped region of the substrate, and (iv) repeating steps (i) through (iii) for a plurality of different selected heights. A plurality of reference data sets are provided characterizing the electrical interaction between the probe tip and the doped region of the substrate as a function of height between the probe tip and the doped region of the substrate. Each data set corresponds to a different dopant density. The measured data set is compared to the plurality of reference data sets and based on the comparison, the dopant density of the doped region of the substrate is determined.

Abstract: A method for amplifying a target nucleic acid is disclosed, which includes: (a) fragmenting a nucleic acid sample to create a target fragment comprising a target nucleic acid and two probe-complementary portions; (b) contacting said fragmented nucleic acid sample with a probe comprising two target fragment-complementary portions complementary to the probe-complementary portions of the target fragment; (c) rendering the fragmented nucleic acid sample single-stranded; (d) allowing the probe-complementary portions to hybridise with the target-fragment complementary portions; (e) if the probe in step (b) is not immobilised, immobilising the probe-target fragment hybrid on a solid phase via immobilisation moiety; (f) separating non-immobilised nucleic acid fragments from the solid phase; (g) contacting the solid phase with a ligase to ligate ligatable 5? and 3? ends of the target fragment whereby the target fragment is circularized; and (h) amplifying said circularized target fragment.

Abstract: A method of deprotecting a solid support bound polynucleotide includes the step of contacting the polynucleotide with a composition comprising a diamine under conditions sufficient to deprotect the 2?-protected ribonucleotide residue. The solid support bound polynucleotide has at least one 2?-protected ribonucleotide residue, which has the following structure: wherein BP is a protected or unprotected heterocycle; R12 is a protecting group selected from a hydrocarbyl, a substituted hydrocarbyl, an aryl, and a substituted aryl; X is O or S; and PG is a thionocarbamate protecting group.

Abstract: A fluid pump for a fluid separation device for separating a fluid includes a fluid inlet being supplyable with fluid at an inlet pressure (pI), and a fluid conducting mechanism configured for conducting the fluid supplied to the fluid inlet towards a connected fluidic path, in which the fluid conducting mechanism is controllable so that, regardless of a value of the inlet pressure (pI), the fluid is continuously conducted away from the fluid inlet with a definable flow rate (FT).

Abstract: The invention provides methods for synthesizing oligonucleotides using nucleoside monomers having carbonate protected hydroxyl groups that are deprotected with ?-effect nucleophiles. The ?-effect nucleophile irreversibly cleave the carbonate protecting groups while simultaneously oxidizing the internucleotide phosphite triester linkage to a phosphodiester linkage. The procedure may be carried out in aqueous solution at neutral to mildly basic pH. The method eliminates the need for separate deprotection and oxidation steps, and, since the use of acid to remove protecting groups is unnecessary, acid-induced depurination is avoided. Fluorescent or other readily detectable carbonate protecting groups can be used, enabling monitoring of individual reaction steps during oligonucleotide synthesis. The invention is particularly useful in the highly parallel, microscale synthesis of oligonucleotides.

Abstract: In a mass spectrometer, a dual stage axial extraction field is applied to transport ions from an accumulator to a detector cell. Ions of a same mass may be transported to the detector cell or a point axially preceding the detector cell at the same time. This may be done by selecting the relative strengths of a first axial electric field applied to the accumulator and a second axial electric field applied to a shutter located at an exit end of the accumulator. This may also be done by selecting relative axial lengths of the accumulator, shutter, and an ion guide located at an exit end of the shutter. A dual stage decelerating field may also be applied to slow ions down prior to and after entering the detector cell.