Abstract: A method and apparatus for performing time of flight mass spectrometry wherein the number of sums of transients taken for generating a given spectra is determined as a function of a characteristic of the incoming data for that spectrum. For instance, the number of transient measurements taken for a given spectrum output can be determined as a function of the abundance of ions in the sample or the abundance of ions corresponding to a base peak or another selected peak. In yet another embodiment, the collection of transients is terminated when a threshold signal to noise ratio is attained.

Abstract: A fluidic device comprising a capillary for conducting a fluid and a pivot arm configured for being pivoted, wherein the pivot arm supports at least a portion of the capillary, and a part of the capillary is coiled to at least partially compensate stress resulting from pivoting of the pivot arm.

Abstract: A waveguide having a spatially-variable refractive index is disclosed. The waveguide having a spatially-variable refractive index comprises a light-propagating medium and a non-uniform distribution of liquid crystal material in a matrix of dielectric material located in a portion of the light-propagating medium.

Abstract: An interface for use in a mass spectrometer is disclosed. The interface comprises a first ion funnel comprising a first inlet and a first outlet, and a first axis between the first inlet and the first outlet. The interface further comprises a second ion funnel in tandem with the first ion funnel, the second ion funnel comprising a second inlet and a second outlet, and a second axis between the second inlet and the second outlet. The first axis and the second axis are offset relative to one another. A mass spectrometer comprising the interface and a method are disclosed.

Abstract: A causal impulse response function is calculated from a truncated spectrum by extending the real part of the spectrum beyond the truncation frequency and computing the imaginary part with the Hilbert transform to enforce causality. The out of band extrapolation is optimized to reduce the discrepancy between the computed and the original imaginary part in the in band frequency range so that the causal impulse response accurately represents the original spectrum. The technique can be applied to spectral with the delay phase subtracted to enforce delay causality. The Hilbert transform may be employed to maintain causality in S-parameter passivity violation correction. At frequencies where violation happens, the S-parameter matrix is scaled down by the inverse of the magnitude of the largest eigenvalue. Magnitudes at other frequencies are unchanged. An additional phase calculated by the magnitude phase Hilbert transform is added to the scaled spectrum to maintain the causality.

Abstract: A scanning probe microscope and method for using the same are disclosed. The Scanning probe microscope includes a probe mount for connecting a cantilever arm and a probe signal generator. The probe position signal generator generates a position signal indicative of a position of the probe relative to one end of the cantilever arm. The probe position signal generator includes a first light source that directs a light beam at a first reflector positioned on the cantilever arm and a detector that detects a position of the light beam after the light beam has been reflected from the first reflector. A second reflector reflects the light beam after the light beam is reflected from the first reflector and before the light beam enters the detector, the second reflector passing light from a second light source that illuminates the sample.

Abstract: In one embodiment, a packet-based system having a number of buses is stimulated using apparatus having 1) a hardware interface configured to provide data packets to the buses; 2) a plurality of hardware-based queue schedulers, each configured to schedule data packets received from a respective one of a plurality of data packet sources, in a respective one of a plurality of hardware-based queues; and 3) a hardware-based priority scheduler configured to cause each particular queue to transmit a next highest priority data packet over one of the buses, based on i) timing requirements of the next highest priority data packet in the particular queue, and ii) a determination that transmission of the next highest priority data packet in the particular queue will not delay a transmission of a higher priority data packet in another one of the hardware-based queues.

Abstract: The present invention discloses novel blends of chimeric and non-chimeric thermostable DNA polymerases for use in PCR, DNA sequencing and mutagenesis protocols. The invention allows for PCR reactions with shorter extension times that will facilitate PCR amplification of genomic DNA templates and improve the efficacy of long PCR.

Abstract: A fluid separation system (10) for separating compounds of a sample fluid in a mobile phase comprises a detector (50) adapted to detect separated compounds by providing an optical stimulus signal to the sample fluid and receiving a response signal to the optical stimulus signal. The detector (50) comprises a light source (100) adapted to provide an output light beam (230) as the optical stimulus signal. The light source (100) comprises a plurality of light emitting elements (200, 200A, 200Z) each adapted to emit a light beam (210, 210A1, 210A2, 210Z1, 210Z2) having a respective wavelength, and a diffracting element (220). The plurality of light emitting elements (200, 200A, 200Z) are arranged that emitted light beams (210, 210A1, 210A2, 210Z1, 210Z2) impinging, on the diffracting element (220) are diffracted by the diffracting element (220) to form the output light beam (230).

Abstract: A Finite Impulse Response (“FIR”) filter that uses an approximation of the time integral of the impulse response derived from data collected at non-uniform intervals and processes time-synchronized measurements taken at uneven intervals.

Abstract: A mass spectrometer system an ion source configured to produce ions and a non-metallic capillary configured to receive at least a portion of the ions from the ion source. The capillary includes an elongated body and multiple bores traversing the elongated body in a longitudinal direction. The bores transport the received ions through the capillary toward a mass analyzer of the mass spectrometer system for detection.

Abstract: Systems and methods for analysis of polymers, e.g., polynucleotides, are provided. The systems are capable of analyzing a polymer at a specified rate. One such analysis system includes a structure having a nanopore aperture and a molecular motor, e.g., a polymerase, adjacent the nanopore aperture.

Abstract: A sample separation device for separating a sample comprises a first fluid supply path for supplying a first fluid, a second fluid supply path for supplying a second fluid, a sample separation unit adapted for separating the sample, a sensor configured for determining a value of a parameter related to the fluid, and a valve configured for selectively coupling both of the first and second fluid supply paths to the sensor and to the sample separation unit, or coupling one of the first and second fluid supply paths to the sensor.

Abstract: The invention provides novel extracts, proteins, and complexes that improve the polymerization activity of nucleic acid polymerases. Included within the aspects of the invention are methods for identifying compositions with a polymerase enhancing activity, methods for purifying and using these compositions, and specific extracts, proteins, and complexes that function to enhance polymerase activity. As an example, specifically described is nucleotide and amino acid sequence information for a Pyrococcus furiousus PEF (P45), which was used to produce a recombinant PEF.

Abstract: The present invention provides an addressable multi-channel peristaltic pump. According to the invention, this pump design allows for selection and operation of one or more pump heads on a drive shaft, while locking other non-selected pump heads in a stationary position. It is possible to operate the multi-channel pump using a limited number of motors, preferably two motors: a selector motor and a dispense motor. Thus, the pump provides for pumping or dispensing of one or more fluids without the need for multiple pumps. Likewise, compared with typical single motor multi-channel systems, where all pump heads on the drive shaft must rotate at the same time, the present invention provides for selective dispensing of one or more fluids. The pumps of the present invention are suitable for any multiple fluid transfer application, including in automated multi-channel reagent dispensing systems, such as nucleic acid purification systems.

Abstract: Embodiments of the present invention provide a method of chromatographic delipidation comprising separating a lipid-containing sample on a superficially porous stationary phase at greater than about 70° C., at least about 80° C., having at least one mobile phase comprising an ion-pairing agent in water, an ion-pairing agent in an organic modifier, an acid in an organic modifier, and an alcohol. The invention provides minimal protein losses and high run-to-run reproducibility. The on-column delipidation method aventageously utilize reversed phase liquid chromatography.

Abstract: An output signal is generated from a received input data stream representing a sequence of digital data values. For each group of successive data values in the sequence of data values, a respective waveform pattern is assigned in dependence of the data content of the respective group of successive data values. The output signal is generated by generating the assigned respective waveform patterns corresponding to the input data stream.

Abstract: A fitting for coupling a tubing to another component of a fluidic device, the fitting comprising a male piece having a front ferrule and a back ferrule both being slidable on the tubing, the male piece further having a first joint element configured slidably on the tubing, and a female piece having a recess configured for accommodating the front ferrule and the tubing and having a second joint element configured to be joinable to the first joint element, wherein the back ferrule is configured in such a manner that, upon joining the first joint element to the second joint element, the back ferrule exerts a pressing force on the front ferrule to provide a sealing between the front ferrule and the female piece, and the back ferrule exerts a grip force between the male piece and the tubing.

Abstract: A thermal conductivity detector includes a structure defining a cavity, the structure principally comprising a material having a first coefficient of thermal expansion; a sensing element for sensing a thermal conductivity of a gas flowing within the cavity, the sensing element having a second coefficient of thermal expansion different from the third first coefficient of thermal expansion, the sensing element being disposed at least in part within the cavity; and a compensation structure having a third coefficient of thermal expansion different from the first and second thermal coefficients of expansion. Over a selected temperature range, a stress within the sensing element is less than a yield stress of any component of the sensing element, and a stress within the compensation structure is less than a yield stress of any component of the compensation structure.

Abstract: A fluidic device for separating different components of a sample, the fluidic device comprising a microstructured body and porous material covering at least a portion of a surface of the microstructured body.