Abstract: Aspects of the disclosure are generally directed to probes and probe compositions for detecting or quantifying a target. One aspect provides a method for selectively hybridizing a probe to a polynucleotide by contacting a sample containing a first and second polynucleotide with a probe. The probe includes a number of nucleotides complementary to the first or second polynucleotide in the region of mismatch between the first and second polynucleotides. Another aspect provides arrays including the disclosed probes and methods of using the arrays and the probes.

Abstract: A dissolution testing apparatus includes a base structure, a vessel support structure, a cleaning manifold and a stirring mechanism. The vessel support structure is configured for mounting testing vessels and is rotatably mounted to the base structure. The vessel support structure is rotatable from an upright position at which respective open ends of the vessels face upwardly to an inverted position at which the open ends face downwardly. The cleaning manifold is mounted to base structure below the vessel support structure and is configured for directing a fluid into the vessels while the vessel support structure is at the inverted position. The stirring mechanism includes shafts and is movably mounted to the base structure. The stirring mechanism is movable from a lower position at which the shafts extend into the vessels to an upper position at which the shafts are outside the vessels. At the upper position the stirring mechanism provides clearance for the vessel support structure to rotate.

Abstract: A coupling device includes a fiber collimator, a wedge window pair and a plane window for coupling a light beam provided by a beam source to optical fiber. The fiber collimator is mounted to a base plate and includes a collimator lens, an end of the optical fiber being positioned at a focal point of the collimator lens. The wedge window pair is mounted to the base plate, and is configured to adjust the light beam to be parallel to an optical axis of the fiber collimator. The plane window is mounted to the base plate between the wedge window pair and the fiber collimator. The plane window is configured to align the parallel direction of the light beam with the optical axis of the fiber collimator.

Abstract: A stacked IC structure has an integrated circuit (IC) having a front IC side, a back IC side, and a first conductive feature formed on the front IC side. A through-chip via connects to the first conductive feature on the front IC side. A substrate has an external circuit formed on a front surface. The IC attaches to the front surface of the substrate and the through-chip via forms a connection between the first conductive feature and the external circuit.

Abstract: Synchronizing multiple-phase data converters by exchanging terminal count pulses via a bidirectional link. Multiple-phase data converters such as analog to digital converters (ADCs) or digital to analog converters (DACs) are synchronized to operate at the same phase by exchanging terminal count (TC) pulses and capturing counter state, representing a time offset from TC. Time offset and the symmetrical delay introduced by the link are used to solve for the delay introduced by the link and the off-set between devices. The offset information is used to align the devices. The process may be repeated to correct for drift.

Abstract: The present invention provides, inter alia, methods of analyzing mass spectral data based on charge states of analyte ions. In some embodiments, the methods can be used for differential profiling of samples, such as comparing a sample comprising a given compound and a sample comprising metabolites of the same compound. The methods can also be used to identify and isolate biomarkers. Systems for performing the methods, as well as computer-readable media for performing the methods, are also described.

Abstract: A system processes discrete digital data according to a function, the discrete digital data including data values and time stamps, the time stamps being at non-uniform time intervals according to a time scale. The system comprises a processor having program instructions for implementing the function in terms of state space equations, using system design and data flow language software; inputting the discrete data into the implementation of the function; and outputting the result as an output discrete data stream of new data values and time stamps.

Abstract: A mass spectrometer comprises an ion detector, an analog-to-digital (A/D) converter, a sample adjuster, and an adder. The A/D converter is configured to generate digital samples representing an analog signal received from the ion detector during a mass scan. The adder is configured to sum the samples with corresponding unsuppressed samples representing analog signals received from the ion detector during previous mass scans, the summed samples defining a mass spectrum. The sample adjuster is configured to identify a peak defined by the samples and to suppress all but one of the samples defining the identified peak to enhance resolution of a peak in the mass spectrum.

Abstract: A flame photometric detector comprises a burner assembly configured to combust a sample of an effluent, the combusted sample emitting at least one excited molecule, an interchangeable selective optical filter configured to pass a plurality of selected optical wavelengths corresponding to the excited molecule, and a photomultiplier tube configured to quantify the concentration of the excited molecule.

Abstract: A column for use in chromatography, the column having a tube with a bore that can contain a packing medium captured between two porous plugs positioned at opposite ends. End fittings are attached to the tube ends, the end fittings having a duct with a duct opening having a diameter at least as large as the diameter of the tube bore which it faces. The duct opening may be positioned within a recess in spaced apart relation to the porous plugs to prevent the end fitting from contacting the plug and burnishing its surface.

Abstract: An RF signal detection process by incorporating direction detection of the RF signal along with energy detection to arrive at a signal of interest (SOI). The SOI is identified by matching direction detections and energy detections. Low-level and noise-like signals can be reevaluated for unresolved direction detections or energy detections. The RF signal detections can be filtered based on valid direction detections very early on in the processing chain to reduce the amount down-stream processing required.

Abstract: A method for deciding the relationship between a physical property and a threshold, comprising measuring a physical property to obtain a measured value, calculating the distribution of actual values for the physical property, calculating the error distribution of the obtained measured value, calculating the penalty associated with an erroneous decision, and calculating a margin based on the expected loss from selecting at least one possible actual value as a function of the calculated distribution of actual values, the calculated error distribution of the measured value, and the calculated penalty associated with an erroneous decision. Embodiments including a system and apparatus are also disclosed.

Abstract: A device for providing a precision current includes a first operational amplifier and multiple path transistors. The first operational amplifier outputs a gate voltage based on reference voltage and input voltages. The path transistors have corresponding gates for receiving the gate voltage from the first operational amplifier. Each path transistor is connected to a first enable transistor configured to selectively connect the path transistor to a reference path and a second enable transistor configured to selectively connect the path transistor to an output path. The first and second enable transistors are separately enabled by first and second enable signals, respectively. At least one path transistor is connected to the reference path through a corresponding first enable transistor to provide the reference current, and least one other path transistor is connected to the output path through a corresponding second enable transistor to provide the precision current based on the reference current.

Abstract: Methods of identifying regions of a nucleic acid sequence suitable for use as probes in nucleic acid arrays, as well as compositions of matter for practicing such methods, are provided. The subject methods include: (a) providing a set of known, undesirable probe sequences; (b) comparing a nucleic acid sequence to each member of the set; and (c) identifying regions of the nucleic acid sequence that substantially match a sequence of a member of the probe set as undesirable regions. The subject methods also include marking the undesirable regions of the nucleic acid sequence to identify regions of the nucleic acid sequence that are suitable for use as probes in a nucleic acid array. The subject invention also includes an algorithm and kits for carrying out the subject methods. Also provided are nucleic acid arrays produced with the identified suitable probes, and methods for using the same.

Abstract: A system for determining signal-to-noise ratio (SNR), signal-to-noise and distortion ratio (SINAD), and total harmonic distortion (THD), includes logic for estimating a frequency of a dominant sinusoidal signal present in a sampled data record, logic for iteratively fitting a curve, the curve including a fundamental sinusoid, harmonic components and a DC offset, and logic for computing SNR, SINAD and THD using the fundamental sinusoid, harmonic components and a DC offset.

Abstract: A discrete-time phase lock loop (DTPLL) includes an analog section comprising a digital-to-analog converter (DAC) and an oscillator, operative to provide a clock signal based on an input from the DAC. The DTPLL also includes a digital signal processor (DSP). The DSP includes a loop controller state machine; a phase detector; a counter, operative to receive clock signals from the oscillator and to provide a count value to the phase detector; a divider, operative to receive a reference signal and to provide a reference pulse output to the phase detector; and a loop filter operative to provide a control effort value based on an output from the phase detector. Based on the phase error value, an output of the oscillator is changed to reduce the phase error to a steady state value.

Abstract: Representative embodiments are directed to distributed sensor systems from which measurement data is extracted using mobile devices. Representative embodiments record access attempts by the mobile devices and calculate the probabilities of future access by a mobile device to sensor devices using the recorded information. Collection points are selected in response to the probabilities of future access. Additionally, memory management and data prioritization may occur that is at least partially dependent upon the probabilities of future access.

Abstract: Power measurements of a signal having a changing frequency are corrected by acquiring samples of the signal, matching the samples of the signal to a sequence of frequencies indexed to correction transforms, and applying the correction transforms to the samples of the signal. Additionally, an apparatus corrects power measurements of a signal having a changing frequency and includes an acquisition block for acquiring samples of the signal, a sequence controller for matching the samples of the signal to a sequence of frequencies indexed to correction transforms and a correction block for applying the correction transforms to the samples of the signal.

Abstract: Methods, tools, systems and computer readable media for compliance testing instrumentation and/or software. Data from one or more analytical instruments and/or software is inputted, and calculations are performed on the data to produce one or more outputs. At least one of the outputs may be compared to first and second test limits, and compliance status of the at least one output relative to the first and second test limits is reported.

Abstract: A fluidic device for providing fluidic connections is described. The fluidic device comprises a fluid conduit and a planar coupling member with a fluid port, the fluid port being fluidically connected with the fluid conduit. A contour of the planar coupling member is in a predefined relationship with the fluid port's position.