Abstract: A biosensor manufacturing method including a sheet material forming process and a dicing process. In the sheet material forming process a sheet material with plural biosensor forming sections is formed. Each of the biosensor forming sections includes a first base plate, a second base plate stacked on the first base plate and forming a capillary between the second base plate and the leading end portion of the first base plate for sucking in sample liquid, and a hydrophilic layer formed on the second base plate at least in a region facing the capillary. In the dicing process plural biosensors are obtained by dicing the sheet material with a blade from the first base plate side at the leading end of each of the biosensor forming sections, such that the leading end of the capillary opens onto the leading end face of the first base plate and the second base plate.

Abstract: A modified pyrroloquinoline quinone glucose dehydrogenase that exhibits a high selectivity for glucose is provided. A modified pyrroloquinoline quinone glucose dehydrogenase is disclosed in which the amino acid residue G at Position 99 of a pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) represented by SEQ ID NO: 1, or the amino acid residue G at Position 100 of the pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) represented by SEQ ID NO: 3, is substituted by the amino acid sequence TGZN (where Z is SX, S, or N and X is any amino acid residue). The modified PQQGDH of the present invention may additionally comprise one or more mutations selected from the group consisting of Q192G, Q192A, or Q192S; L193X; E277X; A318X; Y367A, Y367F, or Y367W; G451C; and N452X (where X is any amino acid residue).

Abstract: Disclosed is a method of amplifying a polynucleotide, comprising: (a) mixing primers for amplifying the polynucleotide, a polymerase, nucleotide substrates and a template polynucleotide, and (b) amplifying the polynucleotide by polymerase reaction, wherein the polymerase has an amino acid sequence consisting of SEQ ID NO: 1 or an amino acid sequence with at least 85% sequence identity to SEQ ID NO: 1, and wherein an amino acid residue corresponding to position 653 of the amino acid sequence has been replaced with glutamic acid.

Abstract: An electrophoresis apparatus that applies voltage from electrodes that are provided in a capillary flow channel and causes component separation by performing electrophoresis on a specimen that is injected into the capillary flow channel comprises: a physical quantity acquisition unit and a physical quantity determination unit. The physical quantity acquisition unit, with migration solution and specimen injected inside the capillary flow channel, acquires an electrical quantity that occurs in the capillary flow channel at a specified time when voltage is being applied to the electrodes. The physical quantity determination unit determines whether or not the electrical quantity that the physical acquisition unit acquires is within a specified range.

Abstract: An analysis system includes: a creatinine information acquiring section to input creatinine measurement data that indicate a measurement quantity of creatinine in a urine sample; a material ingredient information acquiring section to input material ingredient data that indicate a measured quantity of a material ingredient in the urine sample; and a correcting section to correct the measured quantity of the material ingredient indicated as the material ingredient data, by use of the measured quantity of creatinine indicated as the creatinine measurement data.

Abstract: Provided are a measuring apparatus and method for obtaining a measurement value from a response to a signal applied to a sample, wherein the measuring apparatus includes a first measuring unit that measures a first electric response to a first signal that is input to a first pair of electrodes that can come into contact with a sample, a second measuring unit that measures a second electric response to a second signal that is input to a second pair of electrodes that can come into contact with the sample, the second signal changing its value from a first level to a second level and thereafter maintaining the second level for a certain period of time, as a peak value of a response signal with respect to the change in the second signal, and a control unit that corrects a value indicating the amount of a measuring target component of the sample, the value being obtained from the first electric response, based on the peak value of the response signal.

Abstract: Provided are an analytical device comprising a pair of hematocrit electrodes (first pair of electrodes) and a pair of glucose electrodes (second pair of electrodes) that allows a sample to sufficiently reach as far as the second pair of electrodes that are provided on a downstream side in a flow path, a method for manufacturing the analytical device, and a measuring apparatus using the analytical device.

Abstract: The present invention relates to probes which detect a polymorphism(s) in exon 12 of the NPM1 gene, a kit therefor, and the method of detecting the polymorphism(s) thereof.

Abstract: A fluid agitation method is provided, whereby a swirling flow is generated in a trace amount of fluid, thereby agitating the fluid. The fluid agitation method includes introducing the fluid into an agitation chamber (3) including a wall having an uneven mass distribution, and applying oscillation (F) to the wall with frequencies varying in a predetermined frequency range. The uneven mass distribution of the wall is attained, for example, by arranging a plurality of thickened portions (11 to 18) of different thicknesses in a ring.

Abstract: Provided is a method for measuring a color change of an oxidation-reduction indicator, which method is applicable to measurement of the cariogenic bacterial count and the like. A color change of an oxidation-reduction indicator is measured by a method for measuring a color change of an oxidation-reduction indicator, the method comprising reacting a test reagent with a test sample and measuring a color change, wherein the test reagent contains an oxidation-reduction indicator, an oxidation-reduction promoter, and a halogen salt.

Abstract: The present invention provides a nucleic acid collection device that can estimate the nucleic acid collection amount when collecting nucleic acids from a biological sample containing nucleic acids. The nucleic acid collection device comprises a sucking and discharging unit for sucking in and forcing out a sample containing nucleic acids, a collector for collecting the nucleic acids by sucking in and forcing out the sample using the sucking and discharging unit, a pressure measurer for measuring a discharging pressure when forcing out the sample and a sucking pressure when sucking in the sample, and measuring a differential pressure that is the difference between the discharging pressure and the sucking pressure, and an estimator for estimating the collection amount of nucleic acids collected based on the differential pressure.

Abstract: The present invention provides an electrochemical sensor comprising a substrate, an electrically conductive layer made of carbon particles which is disposed on the substrate, a cell membrane mimetic structure layer containing an enzyme at least one of inside the cell membrane mimetic structure layer and on the interface of the cell membrane mimetic structure layer which is disposed on the electrically conductive layer.

Abstract: A sample measuring device according to the present invention includes a measuring unit for performing measurement with respect to a particular component contained in a sample, a measurement data storage unit for storing measurement data obtained by the measuring unit, a display unit for displaying the measurement data, a sensor strip detector for detecting insertion and removal of a sensor strip to which the sample is applied, and a first data transmitter/receiver for transmitting the measurement data via wireless communication. The first data transmitter/receiver performs initial authentication process for wireless communication after insertion of the sensor strip is detected by the sensor strip detector.

Abstract: To provide a cell culture device, a cell culture system and a cell culture method capable of suppressing fluctuations in pressure in a culture fluid, and suppressing gas bubbles from flowing into a cell culture section. A cell culture device including: a cell culture section that cultures cells; a storage section that stores a culture fluid; flow paths that connect the cell culture section and the storage section; a fluid delivery device that is provided at the flow paths and that delivers the culture fluid from the storage section to the cell culture section; a pressure equalizing unit that is provided at the flow paths and that suppresses fluctuations in pressure imparted to the culture fluid delivered to the cell culture section; and a pressurization unit that is provided at the flow path at a flow outlet side of the cell culture section and that applies a specific pressure to the culture fluid.

Abstract: A measurement system includes a measurement device and a multifunctional portable terminal. The a measurement device includes a measurement unit for measuring a particular component in a biological sample, a measurement-side communication unit for transmitting by radio a measurement datum obtained by the measurement unit, and a measurement-side controller for controlling the measurement unit and the measurement-side communication unit. The multifunctional portable terminal includes an output-side communication unit for receiving the measurement datum transmitted from the measurement-side communication unit, an output-side storage unit for storing a plurality of voice message data, an output-side controller for generating a voice message datum or selecting a voice message datum from the voice message data stored in the output-side storage unit based on the measurement datum, and a voice message output unit for outputting the voice message datum generated or selected by the output-side controller.

Abstract: A method for recovering a metal, capable of recovering a metal easily without requiring the use of an organic medium, is provided. A first complex between a first chelating agent and a metal present in a sample is formed in a first mixture prepared by mixing the first chelating agent and the sample. Then, the first complex is recovered from the first mixture, and a second complex between the metal derived from the first complex and a second chelating agent is formed in a second mixture prepared by mixing the first complex and an aqueous solution of the second chelating agent. The aqueous solution is under the pH conditions where the first chelating agent can be insoluble in the aqueous solution. Then, a liquid fraction containing the second complex is recovered from the second mixture. Thus, the metal can be recovered.

Abstract: The one aspect of the present invention aims to provide a novel cloning method for human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), which method is based on culture of dispersed single cells without addition of an apoptotic agent. More specifically, the one aspect of the present invention aims to provide a cloning method for hESCs and hiPSCs based on culture of dispersed single cells utilizing shear stress. The above problem is solved by providing a method for culturing dispersed single pluripotent cells, wherein the dispersed single cells are cultured under laminar flow conditions.

Abstract: A dry test strip for measuring creatinine comprises: a support; a reagent layer that is disposed on the support; a reagent holding layer that is disposed on the reagent layer; and a connection layer that is composed of an adhesive which adhesively bonds the reagent layer to the reagent holding layer and is formed in spot form, wherein the reagent layer contains creatininase and 4-aminoantipyrine; the reagent holding layer contains creatinase, sarcosine oxidase, peroxidase, and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline; and the connection layer delays arrival of a liquid sample spot-deposited on the reagent holding layer at the reagent layer.

Abstract: A measurement device includes: a measurement portion that acquires measurement data; a setting portion that specifies an external device as a transmission destination of the measurement data based on a signal transmitted from the external device, and allows communication with the external device without receiving input of authentication information; and a transmission and reception portion that transmits measurement data to the external device. The setting portion causes the transmission and reception portion to transmit identification information of the measurement device to the external device, and further to transmit confirmation information thereto in addition to the identification information. The confirmation information is used by the external device to decide whether or not the external device can handle the measurement data transmitted from the measurement device.