Abstract: This disclosure describes related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods. Further disclosed are conditions to enable real-time monitoring of RPA reactions, methods to regulate RPA reactions using light and otherwise, methods to determine the nature of amplified species without a need for gel electrophoresis, methods to improve and optimize signal to noise ratios in RPA reactions, methods to optimize oligonucleotide primer function, methods to control carry-over contamination, and methods to employ sequence-specific third ‘specificity’ probes.

Abstract: A method for collecting and processing remotely sensed imagery in order to achieve precise spatial co-registration (e.g., matched alignment) between multi-temporal image sets is presented. Such precise alignment or spatial co-registration of imagery can be used for change detection, image fusion, and temporal analysis/modeling. Further, images collected in this manner may be further processed in such a way that image frames or line arrays from corresponding photo stations are matched, co-aligned and if desired merged into a single image and/or subjected to the same processing sequence. A second methodology for automated detection of moving objects within a scene using a time series of remotely sensed imagery is also presented. Specialized image collection and preprocessing procedures are utilized to obtain precise spatial co-registration (image registration) between multitemporal image frame sets.

Abstract: A MAC design for Ku-band mobile wireless mesh network with multi-beam smart antennas is disclosed. This MAC includes an overlay control that separates the collision domain. It also has lower layer CSMA-like scheme. The disclosed design includes an enhanced PCF and an enhanced DCF for two purposes: (1) exploiting multi-beam concurrent communication capability (2) supporting QoS and mission-based communications. An efficient time synchronization scheme is also disclosed to ensure all beams can concurrently send data to the star node. Finally, ARMA or HMM based prediction schemes are disclosed to predict future traffic profile in each beam. This helps the star node to better prepare the queue content and schedule information.

Abstract: As part of an integrated banking platform, an internet banking platform includes a banking server, a processor, and a memory module. The memory module includes stored computer program code. The memory module, the stored computer program code, and the processor are configured to cause the banking server to receive access to a rewards program server associated with a rewards account platform. Further, the server is caused to receive enrollment information associated with a user, and to connect to the rewards program server. The server is also caused to transmit an enrollment request message to the rewards program server. The enrollment request message includes at least a subset of the enrollment information. Additionally, the server is caused to receive an enrollment response message that indicates whether the user has been enrolled in a rewards account maintained by the rewards account platform.

Abstract: Enables a coffee making apparatus with a froth spoon and at least one impeller configured to enable users to make a cup of coffee, agitate the coffee grounds with the at least one impeller, for example before or after as the plunger is pushed to the bottom of the carafe. Embodiments thus enable a user to make additional froth, pour out the coffee into a cup, and after the cup is filled to the desired level, spoon out froth over the coffee with the froth spoon without pouring excess coffee into the cup.

Abstract: A delivery system and method for a brewed beverage, such as coffee or tea, that stores a batch of brewed beverage in a cooled container, and heats a serving quantity on demand at the point of dispensing. A heater such as a tankless water heater may be connected to a dispensing line to generate hot servings on demand. Cold servings may also be provided. Quality control of the brewing process may be improved by brewing in a centralized, controlled environment and then shipping cooled containers to dispensing points such as coffee shops or cafés. Containers may be filled with a gas such as nitrogen to prevent oxidation, thereby preserving freshness during storage or shipping. At the dispensing site, containers may be refrigerated and attached to gas tanks to maintain the freshness as servings are dispensed.

Abstract: The present invention describes compositions and methods for treating cardiovascular disease and myocardial infarction using dipeptidyl peptidase inhibitors. Also provided are methods for increasing natriuretic peptide function by administering one or more analogues of B type natriuretic peptide that provide increased stability in the presence of prolyl-specific dipeptidyl peptidases.

Abstract: A device and method is described for electronic human prosthetics, and specifically a body-machine interface (BMI) device where the input, output and on-board computing are combined into a single unit to form a compact ECG, respiratory sensing, temperature-sensing-prosthetics device. The devices (BMIs) can also communicate with other body-machine interface devices (BMI) and/or with external controllers wirelessly. The compact device has ultrasonic battery charging system. One or more BMI can be wirelessly connected so that a closed loop of BMIs, or a BMI and an external controller, can wirelessly send trigger pulses to the stimulator over the heart, glossopharyngeal nerve(s) or diaphragm.

Abstract: This application relates to techniques for obtaining wide area intermittent video (WAIV). Some embodiments disclosed herein include a method of obtaining WAIV. The method can include, for example, capturing images at a series of sensor stations having pre-determined locations along a flightline. The flightline can be repeated one or more times, where images are captured at the same sensor stations with each pass of the flightline. The captured images from the same sensor station may have replicated view geometry and may be co-registered and precisely aligned with pixel-level precision. The captured images from multiple sensor stations through time may also be displayed together based upon absolute or relative sensor station locations to create a temporal sequence of wide area intermittent video. The approach provides efficient methods for creating wide area video with reduced temporal imaging frame rates. Systems and devices for forming wide area intermittent video are also disclosed.

Abstract: An electrical service interface system include a configurable device and inverter mounted to an equipment tower, wherein each of the configurable device and inverter include alignment devices configured to rollably mount the configurable device and inverter to corresponding guides mounted to a support pad, and to align the configurable device to the inverter, and the inverter to the equipment tower. In an installed configuration, the configurable device electrically and mechanically couples to the inverter via a DC connector and one or more mounting systems, and the inverter mechanically and electrically couples to the equipment tower via an AC connector and one or more mounting systems.

Abstract: The present invention relates to novel 6-acetylmorphine analogs, and methods for their synthesis and use. Such analogs are designed to provide a convenient linkage chemistry for coupling under mild conditions to a suitable group on a target protein, polypeptide, solid phase or detectable label.

Abstract: Methods and systems for detecting a falling power line in a power transmission or distribution system are described. Electrical measurements at various points in the power distribution system are used to detect changes in a power line indicating that the conductor has broken or become disconnected. Upon detection, control mechanisms are used to deenergize the conductor while the conductor is falling and/or before it contacts the ground.

Abstract: Disclosed herein are systems and methods directed to routing in the wireless mesh network (WMN) with multi-beam directional antennas (MBDAs). The disclosed systems and methods describe Ripple-Diamond-Chain (RDC) shaped routing, systematic link quality modeling and artificial intelligence (AI) augmented path link selection. In simulations, real-time video as well as other types of traffic types are used to validate the high-throughput, quality of service (QoS)-differentiated, multi-beam routing efficiency of the disclosed systems and methods, as well as the intelligent path determination in dynamic WMN environments.

Abstract: Interconnection meter socket adapters are provided. An interconnection meter socket adapter comprises a housing enclosing a set of electrical connections. The interconnection meter socket adapter may be configured to be coupled to a standard distribution panel and a standard electric meter, thereby establishing connections between a distribution panel and a user such that electrical power may be delivered to the user while an electrical meter measures the power consumption of the user. A power regulation module is disposed between the interconnection meter socket adapter, and configured to selectively connect one or more energy sources or energy sinks.

Abstract: Disclosed herein, in certain embodiments, is a method of depositing a polymer onto a surface. In some embodiments, the method comprises using a high electric field and a high frequency vibratory motion to deposit a polymer solution onto the surface. Disclosed herein, in certain embodiments, is a method of manufacturing an electrode or diode. In some embodiments, the method comprises using a high electric field and a high frequency vibratory motion to deposit a polymer onto a surface. Further disclosed herein, in certain instances, is an electrode manufactured by any method disclosed herein. Further disclosed herein, in certain instances, is a diode manufactured by any method disclosed herein.

Abstract: The present invention features novel, diverse, hybrid and engineered recombinase enzymes, and the utility of such proteins with associated recombination factors for carrying out DNA amplification assays. The present invention also features different recombinase ‘systems’ having distinct biochemical activities in DNA amplification assays, and differing requirements for loading factors, single-stranded DNA binding proteins (SSBs), and the quantity of crowding agent employed.

Abstract: A delivery system and method for a brewed beverage, such as coffee or tea, that stores a batch of brewed beverage in a cooled container, and heats a serving quantity on demand at the point of dispensing. A heater such as a tankless water heater may be connected to a dispensing line to generate hot servings on demand. Cold servings may also be provided. Quality control of the brewing process may be improved by brewing in a centralized, controlled environment and then shipping cooled containers to dispensing points such as coffee shops or cafés. Containers may be filled with a gas such as nitrogen to prevent oxidation, thereby preserving freshness during storage or shipping. At the dispensing site, containers may be refrigerated and attached to gas tanks to maintain the freshness as servings are dispensed.

Abstract: Interconnection meter socket adapters are provided. An interconnection meter socket adapter comprises a housing enclosing a set of electrical connections. The interconnection meter socket adapter may be configured to be coupled to a standard distribution panel and a standard electric meter, thereby establishing connections between a distribution panel and a user such that electrical power may be delivered to the user while an electrical meter measures the power consumption of the user. A power regulation module is disposed between the interconnection meter socket adapter, and configured to selectively connect one or more energy sources or energy sinks.

Abstract: The present invention relates to methods for treating systemic inflammation. In certain embodiments, the method comprises administering a therapeutically effective amount of curcumin-selenium loaded nanoparticles. The curcumin-selenium loaded nanoparticles can comprise a matrix of chitosan, polyethylene glycol and tetramethoxysilane encapsulating curcumin and selenium. In certain embodiments, the method comprises administering a therapeutically effective amount of nitric oxide-releasing nanoparticles. The nitric oxide-releasing nanoparticles can comprise a matrix of chitosan encapsulating nitric oxide. In certain embodiments, the systemic inflammation can be caused by endotoxemia. In certain embodiments, the systemic inflammation can be caused by a Filovirus, including an Ebola virus or a Marburg virus. In certain embodiments, the methods for treating systemic inflammation in a subject result in the reduction of proinflammatory cytokines in a subject.

Abstract: In alternative embodiments, provided are chimeric cells, which in alternative embodiments are the so-called “cardiochimeras”, and methods for making and using them. In alternative embodiments, exemplary chimeric cells as provided herein comprise a cardiac stem cell of cardiac origin or a cardiac progenitor cell fused to either a mesenchymal progenitor cell or mesenchymal stem cell, an endothelial progenitor cell or endothelial stem cell, or a cardiac stem cell or a cardiac progenitor cell. In alternative embodiments, the chimeric cells as provided herein comprise an endothelial progenitor cell, which may or may not be of cardiac origin, fused to either a mesenchymal progenitor cell or mesenchymal stem cell, an endothelial progenitor cell or endothelial stem cell, or a cardiac stem cell or a cardiac progenitor cell. In alternative embodiments, methods for making chimeric cells as provided herein further comprise selecting a cell fusion product comprising a viable chimera of the fused cells.