Abstract: The present invention relates to attenuated, immunogenic West Nile virus chimeras built on a dengue virus backbone for the production of immunogenic, live, attenuated West Nile virus vaccines.

Abstract: The system in at least one embodiment provides a tamper evident specimen sample lock that in a further embodiment includes an embedded storage medium. They system and method in at least one embodiment allows for efficiently and securely creating, maintaining, identifying, tracking, and controlling inventories (either prospective or retrospective) of biological and chemical sample containers and their contents, while eliminating potential loss and degradation associated with handling the samples. The system in at least one embodiment provides a sample container tray or cryogenic box having one or more integrated RFID readers within an associated smart lid that sense RFIDs associated with the individual samples thereby eliminating the need to remove, visually inspect, and/or handle the samples.

Abstract: Neutralizing antibodies and antigen binding fragments that specifically bind to Ebola virus glycoprotein are disclosed. Nucleic acids encoding these antibodies, vectors and host cells are also provided. Methods for detecting Ebola virususing the antibodies and antigen binding fragments are disclosed. The antibodies, antigen binding fragments, nucleic acids, and vectors, can be used, for example, to prevent and/or treat Ebola virusinfection in a subject.

Abstract: A synthetic, codon-optimized Hantaan virus (HTNV) full-length M gene open reading frame that consists of a unique nucleotide sequence encoding HTNV proteins. This synthetic gene was cloned into a plasmid to form the first optimized HTNV full-length M gene that elicits neutralizing antibodies in animals when delivered in combination with a similarly optimized Puumala virus (PUUV) DNA vaccine. The invention obviates the need for an extraneous gene sequence that was previously required for expression of the non-optimized HTNV gene. The synthetic gene is engineered into a molecular vaccine system to prevent hemorrhagic fever with renal syndrome (HFRS) caused by infection with HTNV, SEOV, or DOBV. Alternatively, it can be combined with the optimized PUUV DNA vaccine to protect against HFRS caused by any hantavirus.

Abstract: An exoskeletal orthosis includes a proximal cuff including a hinge along an upper edge of the cuff; an ankle section/footplate; and at least one posterior strut connecting the proximal cuff to the ankle section and foot plate.

Abstract: Described in this application is a method for producing Plasmodium sporozoites in a cell-free, mosquito-free in vitro culture-system. Compositions and methods resulting from applying the methods of the present invention are described.

Abstract: Antibodies and method of making antibodies, either monoclonal or polyclonal wherein said antibodies have dual or multi-specific binding capacity to more than one type of antigenic epitope. The antibodies have simultaneous or independent recognition subsites to each of the epitopes. Antigenic epitopes include lipids, peptides, proteins, amino acid sequences, sugars and carbohydrates. Monoclonal antibodies and a method of making monoclonal antibodies of the invention include monoclonal antibodies that are broadly neutralizing to HIV-1 or other envelop viruses wherein the monoclonal antibody has subsites that simultaneously recognize protein and lipid epitopes from the virus.

Abstract: In this application is described a method for preparing nano-VLP composition, thereby permitting purification using chromatography and filtration. The nano-VLP composition has a more uniform size range of filovirus particles, roughly 230 nm diameter, allowing ease of manipulation of the composition, while retaining GP conformational integrity and the antigenic effectiveness of the vaccine. Additionally, the nano-VLP can be lyophilized without loss of nano-VLP structure, or GP immunogenicity. Lyophilized nano-VLP have greatly enhanced thermostability, allowing the creation of a filovirus nano-VLP vaccine without a cold chain requirement.

Abstract: A synthetic molecule 4210 and a therapeutic use of a synthetic small molecule 4210 for treating viral infections, especially encephalitic alphavirus infections. The compound 4210 showed antiviral efficacy by up regulation type 1 interferon (IFN) specifically IFN-?. The compound 4210 was designed and synthesized by a structure-based approach targeting intracellular adaptor protein, myeloid differentiation primary response protein 88 (MyD88). Besides having an antiviral effect, the compound 4210 also demonstrated therapeutic efficacy for treating inflammatory syndrome associated with Gram positive bacterial infections such as exposure to staphylococcal enterotoxin B (SEB) induced toxic shock syndrome (TSS) in mice and can potentially be used in clinical set up for treating sepsis and septic shock.

Abstract: A method of diagnosing TBI-induced tauopathy/chronic traumatic encephalopathy (CTE) by obtaining control samples from a control patients that have not been exposed to TBI and recording a normal range of tissue non-specific alkaline phosphatase (TNAP) or total alkaline phosphatase (AP) activity. Then obtaining samples from object patients that have been exposed to TBI. Comparing the biomarker, TNAP/AP, levels of said object patients to the controls. Then determining if the object patient has been exposed to TBI if the TNAP/AP levels are decreased below the normal range. Treating the patient by increasing the level of TNAP enzyme in the brain to within a normal range or modifying the TNAP enzyme activity so that it regains normal activity.

Abstract: The system in at least one embodiment provides a tamper evident specimen sample lock that in a further embodiment includes an embedded storage medium. They system and method in at least one embodiment allows for efficiently and securely creating, maintaining, identifying, tracking, and controlling inventories (either prospective or retrospective) of biological and chemical sample containers and their contents, while eliminating potential loss and degradation associated with handling the samples. The system in at least one embodiment provides a sample container tray or cryogenic box having one or more integrated RFID readers within an associated smart lid that sense RFIDs associated with the individual samples thereby eliminating the need to remove, visually inspect, and/or handle the samples.

Abstract: Various embodiments associated with synchronization of signal editions from multiple sensors are described. A plurality of sensors can be deployed in an environment. Different copies of a desired signal can be present in the environment and there can be a desire to identify this signal even if the signal is unknown (and a demodulation scheme of the desired signal is not known). The plurality of sensors can sense weak editions of the desired signal and transfer those signals to a master sensor or to a separate location, such as a fusion center. The weak editions may be asynchronous due to the editions being captured at different locations, different channels, different devices, etc. At the master sensor or separate location, the weak editions can be post-synchronized together and then fused together into a strong signal. From the strong signal, a demodulation scheme can be determined and the signal can be demodulated.

Abstract: Grafted triglycerides comprising an acrylated triglyceride grafted with a fatty acid residue containing 4 to 28 carbon atoms. Also described are methods for making a grafted triglyceride and for curing a material selected from vinyl esters and unsaturated polyesters and mixtures thereof and optionally a reactive diluent. The method includes the steps of mixing a grafted triglyceride of the present invention with a material selected from vinyl esters, unsaturated polyesters and mixtures thereof to form a mixture, and curing the mixture to form a cured resin system. A cured resin system comprising a cured product obtained by the foregoing method and composites containing the cured product and a filler or reinforcing material are also disclosed. This method also includes use of the grafted triglycerides to make toughened resin and composite systems with reduced hazardous air pollutants without significantly reducing the glass transition temperature and significantly increasing the viscosity.

Abstract: Various embodiments are described that relate to a loop with a constant phase. The loop can function as an antenna and be used in object detection. A current source can be used to power the loop such that the loop produces a magnetic field and electric field. The magnetic field can be powerful enough to detect a dielectric while the electric field is so small that it does not interfere with nearby communication equipment. Thus, detection can occur without disruption of other important devices.

Abstract: Various embodiments are described that relate to production of an array pattern and an element pattern for an antenna. A user can enter input parameters by way of a computer interface and based on these input parameters the array pattern and element pattern can be produced. The array pattern and the element pattern are produced such that a monitoring apparatus does not identify the antenna as an object of interest when the antenna is implemented with the element pattern and the array pattern.

Abstract: A method and apparatus for path optimization comprising receiving model data, obstacle data and impact data describing an environment, parsing the model and obstacle data and constructing a three-dimensional data grid and calculating the cost of a path traversal from a start node to an end node based on cost of movement and the impact data.

Abstract: Diagnostic biomarkers for diagnosing immune suppression/dysfunction. The diagnostic biomarkers are genes and/or transcripts that are up or down regulated compared to normal expression when a subject has been stressed either mentally and/or physically. The invention also relates to a method of detecting comprised or suppressed immune response in a subject by comparing certain diagnostic biomarkers in the subject to a control set of diagnostic biomarkers.

Abstract: An apparatus and method for inflicting brain injury on a laboratory animal that employs a platform for supporting the laboratory animal. The platform defines an opening for positioning the head of the laboratory animal over the opening. A projectile is launched from a projectile launching device orientated below the opening of the platform. The projectile launching device has a means for propelling the projectile directly at and/or through the opening of said platform, thereby inflicting brain injury on the animal via either a pressure wave or concussive impact of the projectile. Without helmet, direct impact of the projectile results in severe traumatic brain injury. Use of helmet protects animals from skull fracture, subdural hematoma, intracerebral hemorrhage and contusion yet produces mild concussion-like pathology.

Abstract: An electrical component includes an inkjet-printed graphene electrode. Graphene oxide flakes are deposited on a substrate in a graphene oxide ink using an inkjet printer. The deposited graphene oxide is thermally reduced to graphene. The electrical properties of the electrode are comparable to those of electrodes made using activated carbon, carbon nanotubes or graphene made by other methods. The electrical properties of the graphene electrodes may be tailored by adding nanoparticles of other materials to the ink to serve as conductivity enhancers, spacers, or to confer pseudocapacitance. Inkjet-printing can be used to make graphene electrodes of a desired thickness in preselected patterns. Inkjet printing can be used to make highly-transparent graphene electrodes. Inkjet-printed graphene electrodes may be used to fabricate double-layer capacitors that store energy by nanoscale charge separation at the electrode-electrolyte interface (i.e., “supercapacitors”).

Abstract: An electrical component includes an inkjet-printed graphene electrode. Graphene oxide flakes are deposited on a substrate in a graphene oxide ink using an inkjet printer. The deposited graphene oxide is thermally reduced to graphene. The electrical properties of the electrode are comparable to those of electrodes made using activated carbon, carbon nanotubes or graphene made by other methods. The electrical properties of the graphene electrodes may be tailored by adding nanoparticles of other materials to the ink to serve as conductivity enhancers, spacers, or to confer pseudocapacitance. Inkjet-printing can be used to make graphene electrodes of a desired thickness in preselected patterns. Inkjet printing can be used to make highly-transparent graphene electrodes. Inkjet-printed graphene electrodes may be used to fabricate double-layer capacitors that store energy by nanoscale charge separation at the electrode-electrolyte interface (i.e., “supercapacitors”).