Abstract: Techniques Nuc-seq, Div-Seq, and Dronc-Seq are allow for unbiased analysis of any complex tissue. Nuc-Seq, a scalable single nucleus RNA-Seq method, can sensitively identify closely related cell types, including within the adult hippocampus. Div-seq combines Nuc-Seq with EdU-mediated labeling of proliferating cells, allowing tracking of transcriptional dynamics of newborn neurons in an adult neurogenic region in the hippocampus. Dronc-Seq uses a microfluidic device to co-encapsulate individual nuclei in reverse emulsion aqueous droplets in an oil medium together with one uniquely barcoded mRNA-capture bead.

Abstract: Disclosed are a method, a device and a non-transitory storage medium. The method includes: deploying a detection satellite to fly in one detection orbital plane opposite to the satellites in the large-scale constellation, to sequentially detect satellites flying in one target orbital plane of the large-scale constellation in a manner that an approach distance between the detection satellite and a satellite being detected is within a set detection distance, transmitting the detection satellite from the one detection orbital plane to another detection orbital plane in virtue of Earth oblateness perturbation, so as to sequentially detect satellites flying in another target orbital plane of the large-scale constellation in a manner that the approach distance between the detection satellite and a satellite being detected is within the set detection distance, and stopping the detection satellite from detecting until all satellites of the large-scale constellation have been detected.

Abstract: Systems and methods disclosed herein relate to the detection of irregular particles in a blood flow based on a determined relative speed of a particle suspended in a blood flow and/or other properties of a particle suspended in a blood flow including a particle's relative position within a blood vessel and a particles tendency to cluster with other particles suspended in a blood flow. Based on a determined relative speed and/or other relevant factors, the properties of irregular particles may also be measured, including the size, shape, and frequency of irregular particles in a blood flow. Machine learning techniques may be employed to determine patterns for the behavior of irregular particles suspended in a blood flow. These patterns may correspond to particular health risks and conditions.

Abstract: Drawn polymer fibers have internal channels running, at least partially, through the length of the fibers. These fibers may be configured to for use as thermal isolators that can thermally isolate material at the central core of the fiber from the outside environment. In such instances, the channels may be used as insulating channels and/or a heating or cooling fluid can be pumped through the channels to maintain the temperature of the material at the inner core. As another application, the fibers may be used as recuperative, regenerative, parallel-flow, counter-flow, cross-flow or condenser/evaporator heat exchangers. In this case, the channels may be used to direct fluid flow. The fiber may allow for the exchange of heat between fluids in the channels.

Abstract: Described herein are compositions and methods for signaling and antigen-presenting bifunctional receptors (SABRs) comprising one or more antigen presenting domains; and one or more signal transduction domains, wherein the one or more antigen presenting domains comprise a binding fragment of a major histocompatibility complex (MHC) molecule. Various immunological functions of the SABRs are also described.

Abstract: The invention in its various embodiments relates to a method of providing surgical guidance and targeting in robotic surgery systems. The method utilizes data from a navigation system in tandem with 2-dimensional (2D) intra-operative imaging data. 2D intra-operative image data is superimposed with a pre-operative 3-dimensional (3D) image and surgery plans made in the pre-operative image coordinate system. The superimposition augments real-time intraoperative navigation for achieving image guided surgery in robotic surgery systems. Also, a robotic surgery system that incorporates the method of providing surgical guidance and targeting is disclosed. The advantages include minimizing radiation exposure to a patient by avoiding intra-operative volumetric imaging, mobility of tools, imager and robot in and out of the operating space without the need for re-calibration, and relaxing the need for repeating precise imaging positions.

Abstract: Compositions of retroviruses and methods of using the same for gene delivery are disclosed, wherein the retroviruses comprise a viral envelope protein comprising at least one mutation that diminishes its native function, a non-viral membrane-bound protein comprising a membrane-bound domain and an extracellular targeting domain.

Abstract: Systems and methods for determining a property (e.g., density) of a particle (e.g., a cell) are generally described.

Abstract: Systems and methods for determining a density of a particle suspected of having been exposed to a therapeutic agent are generally described.

Abstract: In some aspects, the present disclosure provides methods and compositions for isothermal low temperature amplification of target polynucleotides, and detection thereof.

Abstract: Described is a partitioned cable joint comprising a plurality of physically distributed joint elements with the plurality of joint elements taken together defining a joint length. Joint elements may have a first mounting region having a shape selected to accept one petal of superconducting cable and a second mounting region having a shape selected to accept one petal of a second conductor.

Abstract: A convection enhanced energy storage system includes an electrochemical cell with a positive electrode, a separator, and a negative electrode, a tank holding an electrolyte, and a pump connected to the electrochemical cell and the tank to circulate the electrolyte. The electrochemical cell has large ? and ? values, which has high transport resistance from diffusion and there is limited salt in the electrolyte solution to compensate. A computer system can implement a model of a convection enhanced energy storage system, for example for simulation to select parameters for such an energy storage system. The model includes: a convection term in a Nernst-Planck equation representing the convection enhanced energy storage system; boundary conditions of a cell of the convection enhanced energy storage system to account for forced convection at boundaries; gauging conservation of anions within an external tank; and calculating electrode active area as a function of porosity.

Abstract: The invention provides for systems, methods, and compositions for targeting RNA. In particular, the invention provides a non-naturally occurring or engineered RNA-targeting system comprising an RNA-targeting Cas protein and at least one RNA-targeting guide RNA, wherein said RNA-targeting guide RNA is capable of hybridizing with a target RNA in a cell.

Abstract: An orbital tensile drive is herein disclosed, along with systems and methods associated therewith. The orbital tensile drive uses a tensile element that is conveyed around a static, fixed shaft and a rotating output shaft. This is facilitated via multiple orbiting idler pulleys that are mounted to an orbiter body. The static and rotating shafts, as well as the orbiting assembly, share a common axis. Input rotation to the orbiter body is transformed into lower-speed, higher-torque rotation at the rotating output shaft. The present invention has many potential applications including, but not limited to, robotics.

Abstract: Embodiments provided herein relate to systems for synthetically-engineered reciprocal chromosomal translocation for gene insertion into a population of organisms such as insects.

Abstract: Disclosed are a dark-field confocal microscopic measurement apparatus and method based on vortex dichroism. The apparatus includes an array vortex light generation module, an array vortex light illumination module and an array dark-field confocal detection module; an array vortex wave plate of the array vortex light generation module generates vortex light to illuminate a sample of the array vortex light illumination module; and the array dark-field confocal detection module extracts scattering signals, and identifies differences between scattering signals collected under the illumination of opposite-order vortex light.

Abstract: Methods and systems for the fabrication of composite materials are generally described. Certain inventive methods and systems can be used to fabricate composite materials with few or no defects. According to certain embodiments, composite materials are fabricated without the use of an autoclave. In some embodiments, composite materials are fabricated in low pressure environments.

Abstract: The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered DNA or RNA-targeting systems comprising a novel DNA or RNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA.

Abstract: Systems and methods related to the separation of carbon dioxide (CO2) from a fluid source are generally described.

Abstract: This disclosure describes techniques for fabricating a high-resolution, non-cytotoxic and transparent microfluidic device. A material can be selected based on having an optical property with a predetermined degree of transparency to provide viewability of a biological sample through the microfluidic device and a level of cytotoxicity within a predetermined threshold to provide viability of the biological sample within the microfluidic device. An additive manufacturing technique can be selected from a plurality of additive manufacturing techniques for fabricating the microfluidic device based on the selected material to provide a resolution of dimensions of one or more channels of the microfluidic device higher than a predetermined resolution threshold.