Abstract: The present disclosure provides vectors, methods and kits for for delivery and stable expression of CRISPR/Cas components capable of inducing genetic modification of cells, followed by recombinase-mediated excision of some or all of these components after the cells have been successfully genetically modified. The disclosed vectors and methods provide for reduced immunogenic effects arising from one or more CRISPR/Cas components. The disclosed vectors comprise coding sequences that encode a Cas protein, detectable markers and a guide RNA. The disclosed vectors provide for the subsequent genomic excision of the CRISPR/Cas components after successful genetic modification, as mediated by recombinase recognition of recombination sites flanking one or more of the disclosed coding sequences. The present disclosure further provides methods of generating a population of genetically modified tumor cells for screening a candidate target gene for cancer immunotherapy.

Abstract: An emergency restoration system is disclosed. The emergency restoration system including a base and a tower pivotally connected to the base. The tower including at least one tower section and at least one insulated tower section pivotally connected to the at least one tower section, the insulated tower section including at least one insulator pivotally connected thereto.

Abstract: A system of assessing performance of a photocuring light source includes a photocuring light source unit, a light source control unit, a sensing unit, and a computer unit. The light source unit produces light rays on a work platform of a printing machine. The light source control unit is electrically coupled to and controls the light source unit. The sensing unit is disposed proximally to the light source unit to detect power of the light rays from the light source unit. The computer unit is electrically coupled to the sensing unit and the light source control unit to control the light source unit and record the power of the light rays and operating period of the light source unit. A combination having the system is also disclosed.

Abstract: [Object] To provide a compact, high-output actuator device allowing force control. [Solution] An actuator device 1000 includes an electromagnetic coil member 110 provided over a prescribed width on an outer circumference of a cylinder 100, and a movable element 200 slidable as a piston in the cylinder 100. The movable element 200 has a magnetic member 202, and is moved relatively by excitation of the electromagnetic coil member 110. Fluid is supplied to first and second chambers 106a and 106b such that when the movable element 200 is to be moved relatively, the movable element 200 is driven in the same direction.

Abstract: A magnetic memory structure is provided. The magnetic memory structure includes a magnetic tunneling junction (MTJ) layer and a heavy-metal layer. The MTJ layer includes a pinned-layer, a barrier-layer formed under the pinned-layer and a free-layer formed under the barrier-layer. The heavy-metal layer is formed under the free-layer. The barrier-layer has a first upper surface, the pinned-layer has a lower surface, and area of the first upper surface is larger than area of the lower surface.

Abstract: The present disclosure relates generally to systems, methods, and devices for closed loop deep brain stimulation. In particular, a neural signal is measured and provided to software. The software includes a feature generator and a brain network model that takes the neural signal and estimates other neural signals that are not directly measured, and operates as a model of the brain. The software determines a stimulation signal to be sent to stimulating electrodes. Estimated signals by the brain network model are continuously compared to actual signals from the brain. The closed loop feedback system advantageously allows for electrical stimulation levels and patterns to be continuously updated while delivered to a patient.

Abstract: A method of calculating a moving distance of a movable object in consideration of a bias identified based on a linear velocity of the movable object in the process of implementing a SLAM, and an electronic apparatus therefor are provided. One or more of an electronic apparatus, a vehicle and an autonomous vehicle disclosed in the present invention may be connected to an artificial intelligence module, a drone (Unmanned Aerial Vehicle, UAV), a robot, an augmented reality (AR) device, a virtual reality (VR) device, a device related to 5G service, and so on.

Abstract: A computing system trains a classification model using distributed training data. In response to receipt of a first request, a training data subset is accessed and sent to each higher index worker computing device, the training data subset sent by each lower index worker computing device is received, and a first kernel matrix block and a second kernel matrix block are computed using a kernel function and the accessed or received training data subsets. (A) In response to receipt of a second request from the controller device, a first vector is computed using the first and second kernel matrix blocks, a latent function vector and an objective function value are computed, and the objective function value is sent to the controller device. (A) is repeated until the controller device determines training of a classification model is complete. Model parameters for the trained classification model are output.

Abstract: Some embodiments relate to genetically engineered bacterial strains for modulation of levels of the bacterial metabolite 4-ethylphenol (4EP) and its sulfated form, 4-ethylphenyl sulfate (4EPS). In some embodiments, the bacteria reduce or inhibit production of 4EP or 4EPS in the gut of a subject. The bacteria can ameliorate, delay the onset or reduce the likelihood of one or more symptoms associated with anxiety and/or autism spectrum disorder (ASD) in the subject.

Abstract: Systems and methods improving the performance of statistical model-based single-channel speech enhancement systems using a deep neural network (DNN) are disclosed. Embodiments include a DNN-trained system to predict speech presence in the input signal, and this information can be used to create frameworks for tracking noise and conducting a priori signal to-noise ratio estimation. Example frameworks provide increased flexibility for various aspects of system design, such as gain estimation. Examples include training a DNN to detect speech in the presence of both noise and reverberation, enabling joint suppression of additive noise and reverberation. Example frameworks provide significant improvements in objective speech quality metrics relative to baseline systems.

Abstract: The present disclosure relates to the technical field of non-destructive detecting of residual stress, and in particular to a non-destructive detecting device for component residual stress gradient.

Abstract: A carbon nanotube aligned film as well as a preparation method and application thereof are disclosed. The preparation method includes: providing a carbon nanotube dispersion solution comprising a selected carbon nanotube, a polymer as a carbon nanotube dispersing agent and binding to the selected carbon nanotube, an aromatic molecule binding to the selected carbon nanotube and allowing the surface of the selected carbon nanotube to have the same charges and an organic solvent being at least used for cooperating with the rest components of the dispersion solution to form uniform dispersion solution; and introducing a water phase layer to the upper surface of the dispersion solution to form a double-layer liquid phase system, partially or completely inserting a base into the double-layer liquid system, and then pulling out the base so as to form the carbon nanotube aligned film on the surface of the base.

Abstract: A layered structure having high adhesive properties, and high hardness or excellent transparency, is prepared on a base material such as a resin. A layered structure including: an organic-inorganic hybrid member containing a primary inorganic particle and an organic polymer covalently bound to each other, wherein the primary inorganic particle forms a network containing the polymer; and a particle aggregate layer containing a secondary particle which is deposited on the organic-inorganic hybrid member and is composed of an inorganic material or a metallic material; wherein in the organic-inorganic hybrid member, the primary inorganic particle and the secondary particle have different crystal particle sizes.

Abstract: A vacuum generation system and method utilizes a dual-action piston-cylinder vacuum generation system to evacuate a vacuum storage. Saturated steam of higher than ambient pressure is inserted into a condensation cylinder with two chambers separated by a movable piston. Steam moves the piston to fill one chamber while expel gaseous content and condensate out of the other chamber. Steam is then condensed to a rough vacuum (RV) state by cooling. By repeated operations of inserting and condensing steam in each chamber alternatively, a sustained vacuum generation is achieved. A multi-level vacuum storage is also disclosed with a high vacuum (HV) storage placed inside a rough vacuum (RV) storage to reduce leakage as well as mechanical stresses. The vacuum generation system and method is extended for creating a prime mover or actuator to drive vacuum pumps maximizing thermal energy usage for increased vacuuming capacity.

Abstract: A radial servo control device for a super-resolution optical disc includes an excitation light source, a servo light source, an integrated optical path, focusing units, a servo light detecting unit and a drive control unit; the drive control unit presets N detection error reference values with respect to each guide layer trench irradiated by servo light, and controls corresponding positions of the focusing units in N data tracks below each guide layer trench according to a comparison result between a detection result of servo reflected light and the detection error reference values. The device is applicable to a variety of super-resolution optical discs on the basis of stimulated radiation loss microscopy technology, a two-photon absorption technology, and the like, and achieves accurate radial servo control of super-resolution data tracks (<100 nm) without reducing the wavelength of servo light and the width of guide layer trenches.

Abstract: A 3D optical switch for transferring an optical signal between a plurality of layers of an optical integrated circuit, which comprises: a first optical coupler for distributing the optical signal input to a first optical waveguide deployed in a first layer among the plurality of layers to a second optical waveguide deployed in a second layer different from the first layer; a phase shifter for changing a phase of a first optical signal in the first optical waveguide passing through the first optical coupler and a phase of a second optical signal in the second optical waveguide distributed by the first optical coupler; and a second optical coupler for combining the first optical signal of which the phase is changed and the second optical signal of which the phase is changed is provided.

Abstract: A method for managing AI components installed in containers is provided. The container-based component management method creates a container, installs at least one selected from a plurality of components in the container, and manages the components installed in the container. Accordingly, the execution priorities of the AI components installed in the containers can be managed and operated, such that degradation of system performance and frequent error occurrence can be prevented.

Abstract: An image decoding method according to the present invention includes reconstructing a residual block by inverse-quantizing and inverse-transforming an entropy-decoded residual block, generating a prediction block by performing intra prediction on a current block, and reconstructing an picture by adding the reconstructed residual block to the prediction block, wherein generating the prediction block includes generating a final prediction value of a prediction target pixel included in the current block based on a first prediction value of the prediction target pixel and a final correction value calculated by performing an arithmetic right shift on a two's complementary integer representation for an initial correction value of the prediction target pixel by a binary digit of 1. Accordingly, upon image encoding/decoding, computation complexity may be reduced.

Abstract: The present invention relates to a substrate testing cartridge provided for simultaneously testing multiple substrates for which a substrate treatment process has been finished, and a method for manufacturing same. According to an embodiment of the present invention, a substrate testing cartridge comprises: a chuck member on which a substrate is placed; a probe card which contacts and tests the substrate and is positioned to face the chuck member with reference to the substrate; and coupling members which couple the substrate, the chuck member, and the probe card, wherein each coupling member comprises: a substrate coupling part which couples the substrate and the chuck member; and a chuck coupling part which couples the probe card and the chuck member.

Abstract: The present invention provides compounds of Formula (II), and pharmaceutically acceptable salts, solvates, hydrates, polymorphs, co-crystals, tautomers, stereoisomers, isotopically labeled derivatives, pro-drugs, and compositions thereof. Also provided are methods and kits involving the compounds of Formula (I), (II) or (III) for treating diseases associated with the over-expression of phosphoglycerate dehydrogenase (PHGDH) in a subject, such as proliferative diseases (e.g., cancers (e.g., breast cancer, ER negative breast cancer, melanoma, cervical cancer), benign neoplasms, diseases associated with angiogenesis, inflammatory diseases, autoinflammatory diseases, and autoimmune diseases). Treatment of a subject with a proliferative disease using a compound or composition of the invention may inhibit the activity of PHGDH or inhibit the serine biosynthetic pathway, or both.