Abstract: The present invention provides a polymer replica of a target molecule, the polymer replica being capable of accurately inheriting surface information possessed by the target molecule in a molecular imprinting technique.

Abstract: The invention provides a method for producing a plant cell modified at a targeted site of a double-stranded DNA, including (i) a step of providing a plant cell comprising the double-stranded DNA of interest, (ii) a step of providing a complex in which a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in the double-stranded DNA and a DNA glycosylase with sufficiently low reactivity with the double-stranded DNA are bound, (iii) a step of placing the complex in a condition under which the plant cell is transfected, (iv) a step of placing the transfected plant cell in a condition that induces modification of the targeted site, without cleaving at least one strand of the double-stranded DNA in the targeted site, and (v) a step of selecting a cell into which the complex has been introduced and/or a cell into which the modification has been introduced.

Abstract: An anti-SIRP? antibody that can be used as a tumor agent and an anti-tumor agent comprising the antibody as an active ingredient. An antibody that binds specifically to human SIRP? to inhibit binding of human SIRP? to CD47.

Abstract: A reaction of a fluid in a reaction chamber is satisfactorily performed. A fluid control method includes: generating, in a reaction chamber (84) formed between an outer cylinder (61) and an inner cylinder (70) coaxially disposed in the outer cylinder (61), a reaction phase (30) having a ring shape and formed of a reaction target fluid (F1, F2) in which a Taylor vortex (V1) is generated, the reaction phases (30) arranged in an axis (R) direction, and an inhibiting phase (31) having a ring shape that is adjacent to the reaction phase (30) in the axis (R) direction and inhibits the reaction target fluid (F1, F2) in the reaction phase (30) from flowing out to an outside of the reaction phase (30).

Abstract: To provide a characteristic evaluation device that can properly evaluate a characteristic of a shaft coupling while considering a delay in a response of a motor, a characteristic evaluation device of a shaft coupling includes: a motor system including a drive motor, a rotation angle sensor configured to acquire a rotation angle of a drive shaft, and a motor control unit configured to control the drive motor based on a torque command; a rotational load connected to a driven shaft; and a processor configured to output the torque command and calculate a frequency response of a gain of an amplitude of an angular velocity ? of the rotation angle, wherein the processor is configured to calculate a characteristic of the shaft coupling based on a response characteristic of the motor system and the frequency response.

Abstract: The disclosure provides for agents, compositions of the agent for the use in treating cancer by contacting a cancer cell with an agent to increase the tension of a plasma membrane of the cancer cell, thereby preventing or reducing cell migration and/or proliferation of the cancer cell and treating the cancer.

Abstract: The invention provides a method for producing a plant cell modified at a targeted site of a double-stranded DNA, including (i) a step of providing a plant cell comprising the double-stranded DNA of interest, (ii) a step of providing a complex in which a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in the double-stranded DNA and a DNA glycosylase with sufficiently low reactivity with the double-stranded DNA are bound, (iii) a step of placing the complex in a condition under which the plant cell is transfected, (iv) a step of placing the transfected plant cell in a condition that induces modification of the targeted site, without cleaving at least one strand of the double-stranded DNA in the targeted site, and (v) a step of selecting a cell into which the complex has been introduced and/or a cell into which the modification has been introduced.

Abstract: Provided is a method for altering a targeted site of a DNA in a cell, including a step of stimulating the cell with a factor inducing a DNA modifying enzyme endogenous to the cell, and bringing a complex of a nucleic acid sequence-recognizing module specifically binding to a target nucleotide sequence in a given DNA and a DNA modifying enzyme-binding module bonded to each other into contact with the DNA to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site.

Abstract: A solid-liquid boundary detection device 20 is a device for detecting a boundary 141 between a solid and a liquid in a test tube 14, and includes a surface illumination 21, an imaging unit 22, and a boundary detection processor 31. The surface illumination 21 illuminates the test tube 14 from outside. The imaging unit 22 is arranged on an opposite side of the test tube 14 from the surface illumination 21, and captures an image including the boundary 141. The boundary detection processor 31 detects the boundary 141 from the image captured by the imaging unit 22.

Abstract: Provided is a ??T cell for securing the purity and number of cells sufficient for treatment. Also provided is a method of generating the ??T cell. More specifically, provided are homogeneous ??T cells excellent in that the ??T cells are not affected by exhaustion of the cells. The foregoing is achieved by ??T cells obtained by subjecting induced pluripotent stem cells (iPS cells) to differentiation induction treatment. Specifically, the foregoing is achieved by ??T cells generated by subjecting iPS cells having a rearranged ??TCR gene (??TCR-type iPS cells) to differentiation induction treatment. According to the method of generating the ??T cell of the present invention, there can be provided ??T cells and a cell population of ??T cells that have an excellent function of having antigen-specific cytotoxic activity in a MHC-unrestricted manner, and that are more homogeneous and have a higher effect than ??T cells separated from peripheral blood.

Abstract: The invention provides a site-specific modification method of a DNA molecule that enables gene function manipulation. In particular, the invention provides a method for manipulating gene function by a site-specific action on a target DNA molecule, said method comprising a step for preparing a complex containing an Argonaute protein, a guide RNA and an additional sequence and a step for contacting the target DNA molecule with the complex, wherein the guide RNA is designed to guide the complex to a region containing the desired site in the target DNA molecule.

Abstract: Provided is an orally administrable vaccine against a coronavirus infectious disease. A transformed Bifidobacterium designed to display a part or a whole of a constituent protein of a coronavirus on a surface of the Bifidobacterium enables the provision of the orally administrable vaccine against a coronavirus infectious disease. The transformed Bifidobacterium designed to display a part or a whole of a constituent protein of a coronavirus on a surface of the Bifidobacterium can induce humoral immunity and cellular immunity through oral administration to suppress an increase in severity of pneumonia or the like even after viral infection.

Abstract: Provided is a method capable of shortening the time required for analysis and capable of analyzing various substances produced by microorganisms. A method for analyzing a metabolite of a microorganism according to the present invention includes: a step of supplying a mobile phase including carbon dioxide in a liquid state, a subcritical state, or a supercritical state to a container in which a microorganism cultured in a medium is contained together with the medium, to move a component of a metabolite of the microorganism present in the microorganism and the medium to the mobile phase; a step of introducing a mobile phase to which a component of the metabolite has moved into a column; and a step of performing mass spectrometry on a component of the metabolite contained in the mobile phase that has passed through the column.

Abstract: A holographic three-dimensional multi-spot light stimulation device is provided with: a three-dimensional imaging holographic optical system A which employs fluorescent exciting light to acquire three-dimensional fluorescence distribution information resulting from fluorescent signal light from a plurality of stimulation target objects; and a three-dimensional light stimulation holographic optical system B which employs a light stimulation hologram generated on the basis of the acquired three-dimensional fluorescence distribution information to form a plurality of light spots in space, to impart stimulation simultaneously to the plurality of stimulation target objects.

Abstract: Expression of matrix metalloproteinase 1 is suppressed. A matrix metalloproteinase 1 expression suppression agent of this disclosure contains, as an active ingredient, at least one selected from the group consisting of Saxifraga sarmentosa, an extract of Saxifraga sarmentosa, rosemary, an extract of rosemary, licorice, an extract of licorice, Melissa officinalis, an extract of Melissa officinalis, wild thyme, an extract of wild thyme, hop, and an extract of hop.

Abstract: A federated learning system in which a plurality of local servers repeatedly learn cooperatively through communications between the plurality of local servers and a central server via a network. The local server includes a decryption unit, a mean gradient calculation unit, a model updating unit, a validation error calculation unit, an encryption unit, and a local transmission unit that transmits at least one of a current local mean gradient and a current local validation error. The central server includes a central reception unit, a model selection unit, a weight determination unit, and a central transmission unit. The central reception unit receives encrypted current local models and at least one of current local training data counts, the current local mean gradients, and the current local validation errors from the plurality of respective local servers.

Abstract: The present invention relates to an improved technology for a device that identifies and diagnoses an environmental stress state of plants. An environmental stress diagnosis device comprises a measurement light source 12, an induction light source 14, and a transmitted light detector 18. The measurement light source 12 radiates a first measurement light ML1 and a second measurement light ML2 to a plant sample S, the induction light source 14 radiates a first photosynthesis inducing light FR and a second photosynthesis inducing light AL to the plant sample S, and the transmitted light detector 18 detects a first transmitted light TL1 and a second transmitted light TL2. The control unit 20 has an analysis circuit 20a and a control circuit 20b.

Abstract: The present invention relates to an improved technology for a device that identifies and diagnoses an environmental stress state of plants. A control circuit 20b controls a measurement light source 12 such that a second measurement light ML2 has higher power than a first measurement light ML1 and the first measurement light ML1 and the second measurement light ML2 become opposite-phase rectangular waves, and further controls the measurement light source 12 such that the first measurement light ML1 and the second measurement light ML2 are output in synchronization to form the first measurement light ML1 and the second measurement light ML2 into a quasi-single composite rectangular wave measurement light ML3 of 5 kHz to 30 kHz. A transmitted light detector 18 detects the composite rectangular wave measurement light ML3 transmitted through a plant sample S as a composite rectangular wave transmitted light TL.

Abstract: Provided is a method for altering a targeted site of a DNA in a cell, including a step of stimulating the cell with a factor inducing a DNA modifying enzyme endogenous to the cell, and bringing a complex of a nucleic acid sequence-recognizing module specifically binding to a target nucleotide sequence in a given DNA and a DNA modifying enzyme-binding module bonded to each other into contact with the DNA to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site.

Abstract: The objective of the present invention is to provide a method for producing a carbonyl halide efficiently to the used halogenated hydrocarbon. The method for producing a carbonyl halide according to the present invention is characterized in comprising the steps of preparing a mixed gas comprising oxygen and a C2-4 halogenated hydrocarbon having one or more halogeno groups selected from the group consisting of chloro, bromo and iodo, and flowing the mixed gas and irradiating a high energy light to the flowed mixed gas.