Abstract: The object of the present invention is to provide a coating agent for forming a coating film having barrier properties and stretchability and to provide a resin container comprising a coating film formed using the coating agent on a surface thereof, wherein the coating film has high barrier properties and is less prone to film breakage during stretching. LDH, which is in the form of nanometer-scale particles, is added along with PVA.

Abstract: Providing a membrane-forming solution suitable for producing a separation membrane such as a hollow fiber membrane and a flat membrane. A membrane-forming solution including triacetylcellulose having an acetyl group substitution degree of 2.7 or higher, a good solvent for thermally induced phase separation and a poor solvent for thermally induced phase separation, wherein the good solvent is capable of heat-dissolving the triacetylcellulose (at a solid content concentration of 25 mass %), and the poor solvent is incapable of dissolving the triacetylcellulose up to the heat dissolution temperature of the good solvent, wherein both the good solvent and the poor solvent are included so as to enable phase separation of the heat-dissolved triacetylcellulose solution while the heat-dissolved triacetylcellulose solution is cooled to room temperature (from 20 to 30° C.

Abstract: At least one stable isotope reagent is added to each biological sample and standard sample to prepare biological samples for analysis and standard sample for analysis. The quality of the biological samples is evaluated using data of one set of biological samples for analysis composed of a plurality of biological samples for analysis. Besides, the quality of a pretreatment and/or analysis of each set of samples for analysis is evaluated using data obtained by analyzing the standard sample for analysis before and after an analysis of one set of samples for analysis. An abnormality in a chromatograph or mass analyzer used for the analysis of one set of samples is evaluated by the data obtained by analyzing a sample for device evaluation before and after the analysis of one set of samples for analysis. Thus, the quality of data obtained by chromatographic mass spectrometry on biological samples is comprehensively evaluated.

Abstract: A therapeutic agent for intervertebral disc degeneration that contains LASCol obtained by enzymatically cleaving a terminus of collagen.

Abstract: The present invention provides a method of modifying a targeted site of a double stranded DNA, including a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a selected double stranded DNA and DNA glycosylase with sufficiently low reactivity with a DNA having an unrelaxed double helix structure (unrelaxed DNA) are bonded, with the double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site, without cleaving at least one strand of the double stranded DNA in the targeted site.

Abstract: The present invention provides a complex containing a nucleic acid sequence-recognizing module and a proteolysis tag, wherein the module is linked to the proteolysis tag, the module specifically binds to a target nucleotide sequence in a double stranded DNA, and the tag consists of (i) a peptide containing 3 hydrophobic amino acid residues at the C-terminal, or (ii) a peptide containing 3 amino acid residues at the C-terminal m wherein at least a part of the amino acid residues is substituted by serine.

Abstract: One or more embodiments of the present invention are to provide a new means of improving the productivity of a target protein. The present inventors have identified a novel protein consisting of the amino acid sequence set forth in SEQ ID NO: 2 through an exhaustive analysis of the nucleotide sequence of chromosomal DNA of a yeast belonging to the genus Komagataella. Activation of a gene encoding the novel protein provides a cell having an improved productivity of a target protein.

Abstract: The present invention provides a method of modifying a targeted site of a double-stranded DNA, comprising a step of introducing a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a double-stranded DNA and PmCDA1 are bonded, into a cell containing the double-stranded DNA, and culturing the cell at a low temperature at least temporarily to convert the targeted site, i.e., the target nucleotide sequence and nucleotides in the vicinity thereof, to other nucleotides, or delete the targeted site, or insert nucleotide into the site.

Abstract: A liquid having oxygen absorbing ability, comprising a cobalt-salen complex or a derivative thereof and an ionic liquid formed from an anion having an amine structure and a cation of an aliphatic quaternary phosphonium or ammonium having alkyl chains with each 2-20 carbon atoms, wherein the anion of the ionic liquid is coordinated to a cobalt ion of the cobalt-salen complex or a derivative thereof.

Abstract: A method is for treating a surface of a resin material layer. The method includes a first step of introducing as a substituent at least one selected from the group consisting of an acid halide and an alkyl halide into an aromatic polyether-based resin included in the resin material layer by Friedel-Crafts reaction.

Abstract: A laser tip, a laser treatment tool, a laser treatment device and a laser treatment system are provided for performing laser treatment surgery and minimizing any bleeding. A laser tip is attachable to a laser radiation opening located at the tip end of a laser transmission tube. The laser tip includes an attaching portion detachable from the laser radiation opening and a contact portion contactable with biological tissue. The contact portion is at the front of the attaching portion in the direction in which the laser light exits the laser radiation opening. An open space is located between the contact portion and the attaching portion, and a coupler couples a part of the contact portion and a part of the attaching portion. The contact portion has a reflective surface at a rear end thereof which reflects the laser light toward the coupler.

Abstract: In the present invention: a plurality of meshes are defined on an object surface; the luminance of the object surface when the object surface is viewed from a viewpoint position is calculated for each of the plurality of meshes on the basis of data concerning a processed shape, a surface roughness curve, the viewpoint position, the direction, angle distribution, and intensity of incident light onto the object surface, and a reflectance and scattering characteristics for each wavelength on the object surface; the shape of the object surface is displayed on the basis of the luminances; and data concerning at least the object shape or the surface roughness curve is corrected so as to obtain a desired appearance of the object surface.

Abstract: Provided is an on-chip monitor circuit mounted on a semiconductor chip that is equipped with a security function module for performing a security function process on an input signal and outputting a security function signal, the on-chip monitor circuit comprising a monitor circuit for monitoring signal waveforms of the semiconductor chip, wherein the circuit is provided with a first storage means for storing data that designates a window period in which to perform a test of the semiconductor chip, and a control means for performing control to operate the circuit during the window period, when a prescribed test signal is inputted to the security function module. By using the on-chip monitor circuit in a semiconductor chip of which security is required, security attacks, e.g., a Trojan horse or the like, intended to embed a malicious circuit in the production stage of security function module-equipped semiconductors chips, can be prevented.

Abstract: A shape evaluation method includes a shape error calculation step that calculates a shape error, which is an error between a designed shape and a shape to be evaluated, and a visible error detection step that detects visible shape errors on the basis of the shape error and predetermined visual characteristic data.

Abstract: The invention provides a method of modifying a targeted site of gram-positive bacterium of a double stranded DNA. The method includes contacting the double-stranded DNA with a complex of a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a given double stranded DNA and a nucleic acid base converting enzyme to convert, delete, or insert one or more nucleotides in the targeted site without cleaving at least one strand of the double stranded DNA in the targeted site, by introducing the nucleic acid encoding the complex into the gram-positive bacterium. The invention also provide a nucleic acid-modifying enzyme complex of a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a double stranded DNA of a gram-positive bacterium and a nucleic acid base converting enzyme bonded to each other, which complex is used for the method.

Abstract: The present invention provides a method for modifying a targeted site of a double-stranded DNA in a cell, the method including a step of bringing a complex in which a nucleic acid sequence-recognizing module that specifically binds to a selected target nucleotide sequence in a double-stranded DNA and a nucleic acid base converting enzyme or DNA glycosylase are linked, and a donor DNA containing an insertion sequence into contact with said double-stranded DNA, to substitute the targeted site with the insertion sequence, or insert the insertion sequence into said targeted site, without cleaving at least one strand of said double-stranded DNA in the targeted site.

Abstract: A method of cultivating algal cells of an algae belonging to a class selected from Chlorophyceae, Euglenophyceae, Bacillariophyceae and Haptophyceae includes: irradiating the algal cells with an artificial light having a ratio of (i) photon flux density in a wavelength range of 520-630 nm to (ii) photosynthetic photon flux density, that is 65% or more; and measuring a condition of the algal cells and/or a condition of an algal cell culture provided by cultivating the algal cells. Irradiation and non-irradiation of the algal cells with the artificial light are switched, or the photon flux density in the wavelength range of 520-630 nm is changed, according to the measured condition of the algal cells and/or the measured condition of the algal cell culture.

Abstract: A laminate exhibiting structural color is characterized by including: a base material layer having a plurality of projections or a plurality of recesses substantially regularly arranged on a surface thereof; a first layer that is a metal layer laminated on the surface of the base material layer so as to have a metallic microstructure enabling surface plasmonic resonance; and a second layer laminated on the first layer and made of a material that more easily absorbs visible light than a metal forming the first layer.

Abstract: A digital holographic microscope in which two digital holographic microscopes for detecting a fluorescence image and a phase image, respectively, are combined to be able to three-dimensionally measure a fluorescence image and a phase image at the same time, and perform measurement at a high SN ratio in all the polarization states including random light polarization. A first holographic optical system that, by using laser light, acquires a phase three-dimensional image due to interference light generated by superimposing object light which passes through a sample stage and reference light which does not pass through the sample stage onto each other. A second holographic optical system that, by using fluorescent excitation light, acquires a fluorescence three-dimensional image due to a fluorescence signal light, wherein phase measurement by the first holographic optical system and fluorescence measurement by the second holographic optical system are performed at the same time.

Abstract: A cancer vaccine that can be orally administered can be provided through the use of a transformed Bifidobacterium capable of expressing and displaying a WT1 protein. The WT1 protein expressed and displayed by the transformed Bifidobacterium is a protein covering most of a WT1 protein unlike a WT1 peptide vaccine restricted to a certain HLA. A cancer vaccine using the transformed Bifidobacterium as an active ingredient is applicable to patients of various HLA types.