Abstract: A high efficiency microfluidic biobarrier platform, system, and method permit an in vitro examination of a biobarrier under physiological flow conditions. The platform comprises an inlet, a first member, and an outlet. The inlet receives an influx of fluid. The first member retains the biobarrier, and the member includes a channel in fluid communication with the inlet to receive the fluid and to pass the fluid adjacent to the biobarrier. The outlet directs an outflux of the fluid. The microfluidic platform features built-in plastic electrodes to assess changes in biobarrier impedance in real-time. A system includes the platform, a pump to provide the fluid, a media collecting reservoir to receive the fluid from the platform, and an impedance analyzer to measure changes in biobarrier impedance. A method uses the platform in the system to determine the integrity of the biobarrier and its permeability across the cell barrier.

Abstract: Tetrazine non-canonical amino acids, methods for genetic encoding proteins and polypeptides using the tetrazine amino acids, proteins and polypeptides comprising the tetrazine amino acids, and compositions comprising the proteins and polypeptides having at least one post-translational modification thereof comprising in vivo reaction with the incorporated tetrazine amino acid and a second molecule.

Abstract: This disclosure provides a novel magnetic nanoparticle-assisted genome editing (MAGE) platform, which significantly improves transfection efficiency and biocompatibility in stem cells as well as the efficiency of CRISPR-Cas systems. Due to the great potential of CRISPR-Cas systems, the disclosed MAGE platform can be used for a host of applications in stem cell therapy for genetic disorders.

Abstract: Examples of the present disclosure include a lens. The lens includes a body of a contact lens. The body has a side. The lens includes a plurality of nodes formed onto the side. Each of the plurality of nodes has a parameter value that is based on a reduction of transmission of light through the lens at one or more wavelengths selected to alter a color perception and/or a color discriminability of a wearer of the lens. The parameter value includes a value of: a distance between the node and an adjacent node of the plurality of nodes, a dimension of the node, a configuration of an arrangement in which the plurality of nodes are arranged on the side, a composition of a material coating the node, and/or a material composition of the node.

Abstract: A system including at least one processor is configured for executing a mathematical contagion model based on percolation theory to anticipate the loss distribution resulting from a cyberattack on a class of client-server network architectures with K different client types. The system includes a framework that computes the exact mean and variance of the losses depending on key parameters such as probabilities of attack types, the topology of the network of clients, and contagion strength among the clients. This framework can be used by insurance companies to estimate the liability assessments of insuring a given IT network against the damages that may arise from cyberattacks, including the losses of company revenue, and improved decision-making in cybersecurity protection investments of the network. Further, the framework provides insights into better investment strategies for cybersecurity protection on the client-server network.

Abstract: A flow control system for three-dimensional (3D) control of fluids with a high spatiotemporal resolution using alternating magnetic fields is disclosed. This technology is applicable to many disciplines, including fluid dynamics, microfluidics, electrochemistry, metallurgy, and healthcare. In particular, the proposed technique can address challenging problems such as turbulence control, drag reduction, contactless mixing, crystal growth control, and targeted drug delivery.

Abstract: Using nucleotide architectures to very closely and precisely placed chromophores that produce quantum coherent excitons, biexcitons, and triexcitons upon excitement to create excitonic quantum wires, switching, and gates that would then form the basis of quantum computation. Creating the various excitons and controlling the timing of the excitons would be performed using light of the corresponding wavelength and polarization to stimulate the corresponding chromophores.

Abstract: Systems and methods for utilizing laboratory equipment in a hybrid reality environment or augmented virtual reality environment, are contemplated herein and include: at least one piece of laboratory equipment having at least one feature, wherein the at least one piece of laboratory equipment is tracked; a tracking module for tracking a position and an orientation of the at least one piece of tracked laboratory equipment; a virtual model, stored in a memory, comprising at least one 3-D virtual representation of the at least one feature of the at least one piece of laboratory equipment; and an experimentation module.

Abstract: A technique, and its applications, for high resolution, rapid, and simple nanopatterning. The general method has been demonstrated in several forms and applications. One is patterning nanophotonic structures at an optical fiber tip for refractive index sensing. Another is patterning nanoresonator structures on a sensor substrate for plasmonic effect related detection of VOCs. In the latter example, a graphene oxide coated plasmonic crystal as a gas sensor capable of identifying different gas species using an array of such structures. By coating the surface of multiple identical plasmonic crystals with different thicknesses of Graphene-Oxide (GO) layer, the effective refractive index of the GO layer on each plasmonic crystal is differently modulated when exposed to a specific gas. Identification of various gas species is accomplished using pattern recognition algorithm.

Abstract: The present invention relates to tobacco products from plants comprising mutated berberine bridge enzyme-like nucleic acids and the recessive nic1 and/or nic2 alleles and methods of making the same.

Abstract: Multifunctional dendritic molecular probes that selectively detect Cu2+ ions via potentiometric and fluorometric techniques with low detection limits are disclosed. The selective and reversible binding of the molecules with the Cu2+ ion was used to make solid-state microsensors by incorporating the molecular probes into the carbon-based membranes as an ionophore for Cu (II).

Abstract: The presently disclosed subject matter provides a free-energy based model of translation elongation to predict and optimize heterologous gene expression. The model and software allow for the prediction and optimization of genes for increased or decreased protein yield and for increased or decreased protein aggregation.

Abstract: A biocompatible implant, as well as method of fabricating the implant and repairing an annular defect in annulus fibrosus (AF) of an intervertebral disc (IVD) disposed in a functional spinal unit (FSU), includes an insert that provides faces that can interface with the defect to repair or treat the AF. The implant can deliver biological and mechanical factors to enhance healing in an annular defect and prevent recurrence of herniation symptoms. The implant can be fabricated using a hybrid of first fibrous scaffold structure including at least a first layer and a second layer of first fibers fabricated via three-dimensional fiber deposition (3FD) and second fibrous scaffold structure including a third layer and a fourth layer of second fibers formed via melt electrowriting (MEW). Suitable attachment methods, including bio-adhesives can be used to bond the implant to the IVD and/or FSU.

Abstract: The present application is directed to a compound having the Formula (I): wherein R1, R2, and R3 are as described herein. The present application is also directed to a wax composition comprising a wax and a compound of Formula (I). Processes of making a wax composition and for coating a plant or plant part with the compound of Formula (I) are also described.

Abstract: A device comprises a cannula having a first end, a second end, and a channel between the first end and the second end; an imaging probe couplable to the first end of the cannula, where the imaging probe includes: a transducer, and a reflective surface; and a biopsy device coupled to the cannula, where the biopsy device is configured to collect a tissue sample from an organ of a patient.

Abstract: A method for incorporating semiconductors on a diamond substrate. A buffer layer (e.g., GaN) is grown on a transition layer (e.g., AlN/AlGaN) residing on a substrate. A silicon nitride layer is then grown on the buffer layer. After selectively seeding diamond on the silicon nitride layer, the selective seeding of the diamond is dry etched to form regions with seeded diamond and regions without seeded diamond. The silicon nitride is selectively etched in the regions without seeded diamond and diamond is grown in the regions with seeded diamond forming regions of diamond. Additional Group III-nitride semiconductor material (e.g., GaN) is grown in the etched regions without seeded diamond to fill such regions to reach a level of the regions with diamond. An epitaxial overgrowth of the Group III semiconductor material at and above the level of the regions with diamond is then performed.

Abstract: This disclosure is directed to the discovery of an improved method to diagnose malignant melanoma of the oral cavity in the dog.

Abstract: The disclosure relates to methods and related systems for precision crop modeling and management using the same. Precision crop modeling and management can be incorporated into various methods for growing plants such as crop plants and various methods for managing the growth of such plants in a particular field. The methods generally utilize in-season information relating to weather conditions actually experienced by the field to prepare mid-season, updated crop management plans. A crop management plan is determined using a crop model incorporating a variety of inputs and plant-specific material and energy balances to specify one or more grower-controlled management parameters. An updated plan for a given field can be followed by a grower to increase crop yield and/or optimize one or more other crop or field parameters.

Abstract: In one aspect, the disclosure relates to fluorescent dyes that absorb and emit in the near infrared II (NIR II) range of the electromagnetic spectrum, methods of making same, compositions comprising same and methods of using the compositions to perform imaging on biological samples, and optoelectronic devices using the dyes. The dyes are small organic molecules that are inexpensive and facile to produce, can be water-soluble, have tunable properties, and are biocompatible and/or possess low toxicity.

Abstract: Pharmaceutical compositions of the invention include substituted riluzole prodrugs useful for the treatment of cancers including melanoma, breast cancer, brain cancer, and prostate cancer through the release of riluzole. Prodrugs of riluzole have enhanced stability to hepatic metabolism and are delivered into systemic circulation by oral administration, and then cleaved to release riluzole in the plasma via either an enzymatic or general biophysical release process.