Abstract: The present invention includes compositions and methods for modifying primary T cells. In one aspect, the invention comprises administering to a cell a stem-loop Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) RNA (st-crRNA) and a Cpf1 enzyme.

Abstract: Methods are provided for targeting cells for depletion, including without limitation tumor cells, in a regimen comprising contacting the targeted cells with a combination of agents that modulate immunoregulatory signaling. Immunoregulatory modulating agents include (i) an agent that blockades CD47 activity; and (ii) an agent that agonizes an immune costimulatory molecule, e.g. CD137. The regimen may further comprise an agent that specifically binds to the target cell, e.g. an antibody or biologically active fragment or derivative thereof. The level of depletion of the targeted cell is enhanced relative to a regimen in which a single immunoregulatory modulating agent is used; and the effect may be synergistic relative to a regimen in which a single immunoregulatory modulating agent is used.

Abstract: An insert that has a one-way valve that allows for one-way flow of liquid in response to reduced pressure. The insert is configured to be installed in a baby bottle to form two chambers—a reservoir chamber and a dispensing chamber. The insert also is provided with a valve failure mechanism which allows a user to force the one-way valve open in the absence of reduced pressure, which allows a user to prime the dispensing chamber (the nipple) prior to beginning feeding.

Abstract: Methods and compositions are provided for editing the genome of a cell without the use of an exogenously supplied nuclease. Aspects of the methods include contacting a cell with a targeting vector comprising nucleic acid sequence to be integrated into the target locus, where the cell is not also contacted with a nuclease. In addition, reagents, devices and kits thereof that find use in practicing the subject methods are provided.

Abstract: Compounds comprising peptides capable of binding C3 protein and inhibiting complement activation are disclosed. The compounds comprise compstatin analogs in which the N-terminus and/or C-terminus contains an added component that improves (1) the peptide's solubility at physiological pH; (2) the peptide's plasma half-life; (3) the peptide's intraocular retention; and/or (4) the peptide's binding affinity to C3 or its fragments as compared to an unmodified compstatin peptide under equivalent conditions. Pharmaceutical compositions and methods of using the compounds are also disclosed.

Abstract: Compositions and methods are provided for treating ocular neuropathy in a subject. In one aspect, a recombinant adeno-associated viral vector is provided which includes a nucleic acid molecule comprising a sequence encoding NRF2. In another aspect, a recombinant adeno-associated viral vector is provided which includes a nucleic acid molecule comprising a sequence encoding SIRT1. In desired embodiments, the subject is human, cat, dog, sheep, or non-human primate.

Abstract: Antenna arrays with three-dimensional (3D) conformal radiating elements are provided, as well as methods of manufacturing and methods of using the same. An array can include a ground plane and a plurality of unit cells disposed thereon. Each unit cell can include a 3D conformal radiating element. The 3D conformal radiating elements can be, for example, patches (e.g., circular 3D patches), dipoles, or loops, and each radiating element is conformal on a hemispherical shape.

Abstract: A substrate inspection apparatus includes a light irradiator including an objective lens and a plurality of optical fibers. The objective lens is configured to irradiate light to an illumination area on a semiconductor substrate having a plurality of circuit pattern layers, the plurality of optical fibers are adjacent a periphery of the objective lens and are configured to irradiate the light to a peripheral area adjacent the illumination area. A light generator is configured to generate the light. The light generator is configured to change an irradiation angle of the light to selectively irradiate the light to one or more of the objective lens and the plurality of optical fibers. A light analyzer is configured to obtain images of the circuit pattern layers from the light reflected from the illumination area and the peripheral area.

Abstract: Behavioral and mental health therapy systems in accordance with several embodiments of the invention include a wearable camera and/or a variety of sensors (accelerometer, microphone, among various other) connected to a computing system including a display, audio output, holographic output, and/or vibrotactile output to automatically recognize social cues from images captured by at least one camera and provide this information to the wearer via one or more outputs such as (but not limited to) displaying an image, displaying a holographic overlay, generating an audible signal, and/or generating a vibration.

Abstract: Methods and compositions for 3D and 4D printing inks exhibit desirable electrical and rheological properties. The compositions disclosed include a 3D printing ink employing a liquid metal emulsion and a method of creating a stretchable electronic device using the same. The compositions also include 4D printing inks and methods of tuning said inks to have desirable properties.

Abstract: A method for tomographic imaging comprising acquiring [200] a set of one or more 2D projection images [202] and reconstructing [204] a 3D volumetric image [216] from the set of one or more 2D projection images [202] using a residual deep learning network comprising an encoder network, a transform module and a decoder network, wherein the reconstructing comprises: transforming [206] by the encoder network the set of one or more 2D projection images [202] to 2D features [208]; mapping [210] by the transform module the 2D features [208] to 3D features [212]; and generating [214] by the decoder network the 3D volumetric image [216] from the 3D features [212]. Preferably, the encoder network comprises 2D convolution residual blocks and the decoder network comprises 3D blocks without residual shortcuts within each of the 3D blocks.

Abstract: The present disclosure provides compounds represented by Formula I: and pharmaceutically acceptable salts, solvates, e.g., hydrates, and prodrugs thereof, wherein X and n are as defined as set forth in the specification. The present disclosure also provides compounds of Formula I for use to treat diseases and conditions, e.g., cancer, wherein inhibition of HDAC provides a benefit.

Abstract: Systems and methods for training photonic neural networks in accordance with embodiments of the invention are illustrated. One embodiment includes a method for training a set of one or more optical interference units (OIUs) of a photonic artificial neural network (ANN), wherein the method includes calculating a loss for an original input to the photonic ANN, computing an adjoint input based on the calculated loss, measuring intensities for a set of one or more phase shifters in the set of OIUs when the computed adjoint input and the original input are interfered with each other within the set of OIUs, computing a gradient from the measured intensities, and tuning phase shifters of the OIU based on the computed gradient.

Abstract: Disclosed herein are compounds, compositions, and methods for selecting targeting ?2AR receptors. The compounds, compositions, and methods may be used in the treatment of obstructive lung diseases.

Abstract: The invention provides a polynucleotide sequence (e.g., a gene, e.g., DNA or RNA) encoding UGT1A1 (e.g., expressing human UGT1A1). The invention further provides a vector, such as an adeno-associated virus (AAV) vector (e.g., AAV8) having a vector genome including inverted terminal repeat sequences and a UGT1A1 coding sequence operably linked to one or more expression control sequences (e.g., expression control sequences including a liver-specific promoter). Also provided are compositions containing these AAV vectors and methods of treating Crigler-Nijjar syndrome type I, Crigler-Nijjar syndrome type II, and Gilbert syndrome.

Abstract: The present disclosure relates to devices and methods for the detection and/or sorting of nucleic acids. Further disclosed are methods for device fabrication.

Abstract: Immunogenic compositions and methods of their use in eliciting immune responses to coronaviruses, such as SARS-CoV-2 are provided.

Abstract: A method for cardiac computed tomography imaging acquires 4D image volumes using cardiac CT, generates synthetic low noise data from the 4D image volumes for a complete cardiac cycle by exploiting redundancies in the data, uses the generated synthetic low noise data to train a 3D U-Net with 2D+time architecture to produce denoised cardiac CT output from noisy cardiac CT input, and uses the trained 3D U-Net for deep learning based denoising of functional cardiac CT data.

Abstract: This invention provides systems and methods that can detect incidental OVFs in CT examinations at the level of practicing radiologists. The OVF detection system leverages a deep convolutional neural network (CNN) to extract radiological features from each CT scan slice. These extracted features are processed through a feature aggregation module to make the final diagnosis for the full CT scan. Feature aggregation, can be performed in a variety of ways, including the use of a long short-term memory (LSTM) network. In one example, the CNN can be trained on a predetermined number of CT scans, which are established as reference standards. This result can effectively the performance of practicing radiologists on this test set in real world clinical circumstances. The system and method can be employed to assist and improve OVF diagnosis in clinical settings by pre-screening routine CT examinations and flagging suspicious cases prior to review by radiologists.

Abstract: The present disclosure generally relates to compositions and methods useful for an adoptive cell therapy. Some embodiments of the disclosure relates to methods for producing a population of lymphocytes enriched in T stem cell memory cells (TSCM cells). Further provided are compositions containing a substantially pure population of TSCM cells that are therapeutically effective in treating various cancers, and kits containing such compositions. Methods for treating a subject having or suspected of having a cancer using the compositions and kits as disclosed herein are also provided.