Abstract: The present application provides methods for preparing soluble lipidated ligand agents comprising a ligand entity and a lipid entity, and in some embodiments, provides relevant parameters of each of these components, thereby enabling appropriate selection of components to assemble active agents for any given target of interest.

Abstract: Various aspects as described herein are directed to a radiative cooling apparatuses and methods for cooling an object. As consistent with one or more embodiments, a radiative cooling apparatus includes an arrangement of a plurality of different material located at different depths along a depth dimension relative to the object. The plurality of different material includes a solar spectrum reflecting portion configured and arranged to suppress light modes, thereby inhibiting coupling of the incoming electromagnetic radiation, of at least some wavelengths in the solar spectrum, to the object at a range of angles of incidence relative to the depth dimension. Further, the plurality of material includes a thermally-emissive arrangement configured and arranged to facilitate, simultaneously with the inhibiting coupling of the incoming electromagnetic radiation, the thermally-generated electromagnetic emissions from the object at the range of angles of incidence and in mid-IR wavelengths.

Abstract: Incorporation of deuterium into the lipids, proteins, and/or genetic material in cells, tissues, organs, or organisms, including animals, such as humans, and plants, by administration of heavy water, allows for the spectral determination of levels of lipids, proteins, and/or genetic material using Raman spectroscopy, such as stimulated Raman spectroscopy. Following administration of a drug, changes in the relative amounts of lipid, protein, and/or genetic materials can be determined, and the efficacy, or lack thereof, of the administered drug can be determined.

Abstract: The disclosure provides compositions and methods for high-efficiency genome editing in mammals. In some aspects, the disclosure provides guide RNA-retron cassettes, RNA-guided nuclease (e.g., Cas9)-reverse transcriptase fusion protein encoding cassettes, and vectors comprising the cassettes. Also provided are mammalian host cells that have been transfected with the vectors. In other aspects, guide-retron donor DNA molecules are provided. In some other aspects, methods for genome editing in mammals and the screening of genetic loci are provided. In further aspects, methods and compositions are provided for the prevention or treatment of genetic diseases and for other applications. Kits for genome editing and screening are also provided.

Abstract: According to various embodiments, a system for using magnetic reconnection to accelerate plasma is disclosed. The system includes a pair of electrodes including two concentric rings separated by an electrode gap and held at different electrostatic potential by applying a voltage to generate an inter-electrode electric field. The system further includes a plurality of magnetic coils configured to produce magnetic field lines that connect the pair of electrodes. The system additionally includes a gas injector configured to inject gas into the electrode gap, the injected gas being partially ionized by the inter-electrode electric field to generate a poloidal current that flows along open magnetic field lines across the electrode gap.

Abstract: The harmonic tag includes a ring resonator circuit and may include a first ring microstrip disposed on a first side of a first dielectric substrate, a microstrip feedline spaced apart from and adjacent to the first ring microstrip and a ground plane disposed opposite the first ring microstrip and the microstrip feedline on a second side of the first dielectric substrate. The ring resonator circuit is tuned to resonate at an interrogation frequency. A dipole antenna circuit may include a dipole microstrip electrically coupled to the microstrip feedline through a first diode. The first diode causes the dipole microstrip to transmit a harmonic signal having a harmonic frequency twice the interrogation frequency.

Abstract: A computer that includes a processor and a memory, the memory including instructions executable by the processor to generate radar data by projecting radar returns of objects within a scene onto an image plane of camera data of the scene based on extrinsic and intrinsic parameters of a camera and extrinsic parameters of a radar sensor to generate the radar data. The image data can be received at an image channel of an image/radar convolutional neural network (CNN) and receive the radar data at a radar channel of the image/radar CNN, wherein features are transferred from the image channel to the radar channel at multiple stages Image object features and image confidence scores can be determined by the image channel, and radar object features and radar confidences by the radar channel. The image object features can be combined with the radar object features using a weighted sum.

Abstract: Nanocmulsion compositions, processes, and methods that include dihydromyricetin (DHM).

Abstract: Compounds and compositions for the transient expression of exogenous telomerase activity in a cell are provided. The compounds and compositions, which relate to a ribonucleic acid coding for a telomerase reverse transcriptase, are useful in the extension of telomeres in cells needing such treatment. Such cells include, for example, cells that contain shortened telomeres and cells from subjects that may benefit from telomere extension, for example subjects that suffer from, or are at risk of suffering from, age-related or other illnesses. Also provided are methods of extending telomeres through the administration of the provided compounds and compositions to animal cells, either in vitro or in vivo, and kits including the compounds or compositions and instructions for use.

Abstract: In one aspect, the disclosure relates to a facile strategy to introduce electron-deficient aryl sulfate diesters to silylated hydroxyl groups of carbohydrates and amino acids, among other substrates, wherein selective hydrolysis and the removal of an electron-deficient aromatic group allows for the efficient generation of sulfated carbohydrates, peptides, and other compounds. The incorporation of electron-deficient aryl sulfate diesters in the early stage of the synthesis of glycans, peptides, and the like, disclosed herein avoids time-consuming protecting group manipulations, simplifies the purification of sulfated products, and improves the overall yield and efficiency. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

Abstract: This invention provides purified preparations of an RNA, oligoribonucleotide, or polyribonucleotide comprising a modified nucleoside, and methods of assessing purity of purified preparations of an RNA, oligoribonucleotide, or polyribonucleotide comprising a modified nucleoside.

Abstract: A flexible sensor is provided. A further aspect employs a sensor apparatus including pressure or force sensing layers or sheets in addition to location sensing layers or sheets. In another aspect, an electrical sensing circuit and flexible films are used to sense pressure and/or location of a contacting object. In another aspect, a compressible polymer includes conductive particles therein such that the compressible polymer changes electrical resistance when compressed to indicate a force or location. Still another aspect has stacked sandwiches of pressure and/or location sensing films, layers or sheets.

Abstract: Smart membranes (14) are integrated into a small, unmanned surface vessel (20) to enable the efficient, automated cleanup of oil spills. Such a vessel (20) has the potential to provide a low-cost, modular solution for day-to-day oil-spill cleanup operations, especially in confined aquatic areas, such as under piers and in the small spaces between marine vessels and piers. The smart membranes (14) are provided on the surface of a conveyor belt (34) that circulates the membranes (14) through the surrounding body of water (10) for oil collection, as well as through an internal reduction chamber (22) of the vessel (20) for oil release. The smart membranes (14) are adapted to attract and repel oil (12) in response to low-voltage commands applied across the conveyor belt (34), using a process that is repeatable for a number of cycles, offering high efficiency and long durability (FIG. 5).

Abstract: The present invention describes substituted benzo[f]imidazo[1,5-a][1,4]diazepines of the formula (I): wherein R1, R2, R2?, R3, and X are as disclosed herein. These compounds target alpha-4 and alpha-5 GABAa receptors and are useful for the treatment of airway hyperresponsiveness and inflammation in asthma.

Abstract: Disclosed is an assay for determining resistance in a target cell or tissue to a therapy associated with cellular stress using chemical microscopy and high-throughput single cell analysis to determine functional metabolic alteration, including determining metabolic reprogramming in a target cell or tissue to a therapy associated with cellular stress, and methods of using the assays.

Abstract: Cyclooxygenase-2 (COX-2) over-expression is prominent in inflammatory diseases, neurodegenerative disorders, and cancer. Directly monitoring COX-2 activity within its native environment poses an exciting approach to account for and illuminate the effect of the local environments on protein activity. Herein, we report the development of CoxFluor, the first activity-based sensing approach for monitoring COX-2 within live cells with confocal microscopy and flow cytometry. CoxFluor strategically links a natural substrate with a dye precursor to engage both the cyclooxygenase and peroxidase activities of COX-2. This catalyzes the release of resorufin and the natural product, as supported by molecular dynamics and ensemble docking. CoxFluor enabled the detection of oxygen-dependent changes in COX-2 activity that are independent of protein expression within live macrophage cells.

Abstract: A reflector apparatus is provided. A further aspect employs at least one antenna or radar operably emitting signals, and a moving object-mounted reflector which operably reflects at least some of the signals back to the at least one antenna or radar, the reflector including trihedral walls with metallic reflective surfaces. In another aspect, a programmable controller in an automotive vehicle determines a moving object-characteristic based on at least reflected signals received by a horn antenna using a Doppler or micro-Doppler effect. Still another aspect includes a trihedral reflector which moves with a movable object including handlebars, a straddled user seat and being one of: a bicycle, a motorcycle, or an all-terrain vehicle. A further aspect includes multiple spaced apart trihedral reflectors attached to a user-wearable, flexible and nonconductive substrate.

Abstract: Aspects provide fluid responsive actuators and methods of using fluid responsive actuators capable of generating a power-to-volume ratio sufficient to inject a drug from a syringe within an injection period from about 1 to about 60 seconds after exposure of the fluid responsive elements to a fluid. The fluid responsive actuators can be used to inject viscous drugs to a patient.

Abstract: The present disclosure relates to, among other things, compositions and methods for treating an inflammatory condition including, but not limited to, ocular inflammation, dry eye disease, and ocular neuropathic pain. One aspect of the present disclosure relates to a composition comprising (a) chemerin or a fragment or analog thereof and (b) a lipid entity linked to the chemerin or fragment or analog thereof.

Abstract: Aspects of the present disclosure generally relate to catalyst compositions including metal chalcogenides, processes for producing such catalyst compositions, processes for enhancing catalytic active sites in such catalyst compositions, and uses of such catalyst compositions in, e.g., processes for producing conversion products. In an aspect, a process for forming a catalyst composition is provided. The process includes introducing an electrolyte material and an amphiphile material to a metal chalcogenide to form the catalyst composition. In another aspect, a catalyst composition is provided. The catalyst composition includes a metal chalcogenide, an electrolyte material, and an amphiphile material. Devices for hydrogen evolution reaction are also provided.