Abstract: This present disclosure relates to a bioengineering approach based on microphysiological culture to mimic tissue-tissue interface. Accordingly, the present disclosure provides methods, compositions and kits related to the approach.

Abstract: Host cells that are engineered to produce benzylisoquinoline alkaloid (BIAS) precursors, such as norcoclaurine (NC) and norlaudanosoline (NL), are provided. The host cells may have one or more engineered modifications selected from: a feedback inhibition alleviating mutation in a enzyme gene; a transcriptional modulation modification of a biosynthetic enzyme gene; an inactivating mutation in an enzyme; and a heterologous coding sequence. Also provided are methods of producing a BIA of interest or a precursor thereof using the host cells and compositions, e.g., kits, systems etc., that find use in methods of the invention.

Abstract: The subject matter described herein relates to laminated magnetic cores, methods of fabricating laminated magnetic cores, and electric devices using laminated magnetic cores. In some examples, a method for fabricating a laminated magnetic core includes depositing a first magnetic layer and depositing an interlamination layer of over the first magnetic layer. The interlamination layer comprises a partially conducting material having a conductivity greater than or equal to 10?4 S/cm and less than or equal to 105 S/cm. The method includes depositing a second magnetic layer over the interlamination layer. The method can include sequentially depositing additional interlamination layers and additional magnetic layers in an alternating fashion to produce the laminated magnetic core.

Abstract: A laser system for blood assessment is provided. Another aspect of a laser system includes a container within which is blood, a laser operable to emit a laser beam at the container to vibrate the blood, a detector operable to detect a vibrational characteristic of the blood, a controller connected to the detector operable to automatically determine a characteristic of the blood based at least in part on the vibrational characteristic detected by the detector, and a display operable to indicate a determination result from the controller. Another aspect of the present blood or tissue assessment laser system includes a laser, a power supply, a detector, a controller and an electronic display, all contained within or coupled to a handheld and portable housing.

Abstract: Methods for performing frequency and phase correction of magnetic resonance spectroscopy (MRS) data in quantifying one or more metabolites involved in the pathology of schizophrenia and related disorders.

Abstract: A dual vector system for treating a genetic disorder is provided. The system includes (a) a gene editing vector comprising an expression cassette comprising a nucleic acid sequence encoding a nuclease and regulatory sequences that direct expression of the nuclease in a target cell comprising a PCSK9 gene; and (b) a donor vector comprising a nucleic acid sequence encoding an exogenous product for expression from the PCSK9 locus, wherein the inserted nucleic acid sequence does not encode PCSK9, wherein the system further comprises sequences that direct the nuclease to specifically targets the native PCSK9 gene locus; and wherein the native PCSK9 in the target cell is optionally ablated or reduced post-dosing with the dual vector system.

Abstract: Disclosed are methods, systems, device, and other implementations, including a method (performed by, for example, a hearing aid device) that includes obtaining a combined sound signal for signals combined from multiple sound sources in an area in which a person is located, and obtaining neural signals for the person, with the neural signals being indicative of one or more target sound sources, from the multiple sound sources, the person is attentive to. The method further includes determining a separation filter based, at least in part, on the neural signals obtained for the person, and applying the separation filter to a representation of the combined sound signal to derive a resultant separated signal representation associated with sound from the one or more target sound sources the person is attentive to.

Abstract: The invention provides aminoacyl-tRNA synthetases, compositions thereof, and methods for use thereof.

Abstract: Compositions and methods for eliciting an immune response in a subject against Vibrio cholerae are provided. The compositions include fusion proteins with one or more epitopes from Vibrio cholerae, nucleic acids and vectors encoding the fusion proteins, pharmaceutical compositions, immunogenic compositions, and vaccines. In examples, the methods include administering a disclosed composition to a subject, such as a human.

Abstract: Provided herein are compounds, pharmaceutical formulations, and methods for treatment of cancer, particularly including chronic myeloid leukemias, and neurodegenerative disorders in a subject.

Abstract: The technology described herein is directed to compositions and methods for modifying and controlling the activity of cells by expression of proteins from self-amplifying RNA (saRNA). Also described herein are compositions and methods for modifying and controlling the activity of cells by expression of proteins from self-amplifying RNA that is substituted with chemically modified nucleotides.

Abstract: Embodiments of the present disclosure pertain to compositions that include a metal-organic framework. The metal-organic framework includes a plurality of metals and a plurality of ligands coordinated with the plurality of metals, where the plurality of metals include bismuth, and the plurality of ligands include a plurality of hydroxy moieties that are not part of carboxyl groups. At least some of the hydroxy moieties are coordinated with bismuth to form Bi—O bonds. The metal-organic framework is in the form of a conductive and interconnected network. Additional embodiments of the present disclosure pertain to methods of utilizing the compositions to detect analytes in a sample and shield various objects from radiation.

Abstract: Disclosed is a fast-response sensor system to measure a fluid vector in a plurality of dimensions. This system, known as ‘MAST’ (for MEMS Anemometry Sensing Tower), utilizes microelectromechanical (MEMS) hot-wire devices to produce a solid-state, lightweight, and robust flow sensor system suitable for, e.g., real-time wind estimation onboard a UAV. The MAST uses three through eighteen polygonally-arranged microscale flow sensors to determine the fluid's vector's direction and magnitude.

Abstract: This disclosure provides data showing that LEFTY inhibits differentiation-promoting pathways such as BMP7/pSMAD5 in breast cancer cell lines, over and above its known role of inhibiting Nodal/pSMAD2. LEFTY competes with BMP7 to bind to its cell surface receptor BMPR2, leading to inhibition of pSMAD5. The LEFTY-BMPR2 interaction is dominant over BMP-BMPR2 in tumorigenic cells, resulting in diminished pSMAD status, whereas in non-tumorigenic cells, there is minimal LEFTY-BMPR2 interaction, increased BMP7-BMPR2 association, and elevated pSMAD. Compositions and methods for inducing or inhibiting expression and/or the activity of LEFTY and BMP proteins are described, which can be used in diagnosis and therapy of cancer and other conditions, and to promote proliferation of stem cells.

Abstract: Devices, systems, and methods for shock absorption are provided herein. Collapsible shock absorption devices have an inner wall having at least one orifice, an outer wall, and a fluid sealed within the outer wall can mitigate sharp increases in force during loading and can better distribute loading forces. In some cases, collapsible shock absorption devices disclosed herein are used for prevention of injury to a biological tissue of a subject or damage to an inanimate object.

Abstract: An acoustic resonator includes a piezoelectric thin film (PTF) disposed on a carrier substrate. The PTF confines a fundamental shear-horizontal (SH0) surface-acoustic wave (SAW) within the PTF. The acoustic resonator includes an input bus line coupled to an input source and a ground bus line coupled to a ground potential. The acoustic resonator includes a first grating reflector disposed at a first end of the PTF and coupled between the input bus line and the ground bus line. The acoustic resonator includes a second grating reflector disposed at a second end of the PTF and coupled between the input bus line and the ground bus line. The acoustic resonator includes interdigital transducers (IDTs) disposed between the first grating reflector and the second grating reflector. Each IDT includes an input electrode coupled to the input bus line, and a ground electrode coupled to the ground bus line.

Abstract: In an example, a method including dispensing a liquid onto a first portion of a surface of a substrate and dispensing a solution comprising colloidal spheres onto a second portion of the surface of the substrate. The method additionally includes agitating the colloidal spheres to disperse the colloidal spheres along the first portion and the second portion of the surface of the substrate and directing air flow above the colloidal spheres inducing rotation of the colloidal spheres. In another example, a method includes positioning a retaining ring on a surface of a liquid above a substrate below the surface of the liquid and dispensing a solution comprising colloidal spheres onto the surface of the liquid within a surface area of the retaining ring. The method further includes agitating the surface of the liquid and the colloidal spheres to disperse the colloidal spheres along the surface area of the retaining ring.

Abstract: Disclosed herein are Fenton filters comprising a porous substrate and a catalyst coating the porous substrate, wherein the catalyst includes a matrix and single metal atoms incorporated in the matrix. Also disclosed herein are methods of generating radicals from an oxidant, electrolyzers, methods of generating hydrogen peroxide, and water treatment systems.

Abstract: A method of fabricating a sensing device for DNA sequencing and biomolecule characterization including the steps of fabricating a microelectrode chip having a silicon substrate and a silicon nitride diaphragm, attaching a monolayer graphene sheet to the silicon nitride diaphragm, dicing a portion of the monolayer graphene sheet to form a graphene microribbon, converting the graphene microribbon to a graphene nanoribbon, and converting the graphene nanoribbon to a carbyne. A sensing device for DNA sequencing and biomolecule characterization is also disclosed. The sensing device includes a silicon substrate, a cavity in the silicon substrate covered by a silicon nitride layer, microelectrodes attached to the silicon nitride layer, graphene covering the microelectrodes, and carbyne attached to a portion of the silicon nitride layer covering said cavity.

Abstract: Systems and methods for synthetic image generation include a method of generating synthetic histological slide images that includes translating each of several RNA-Seq records into a latent space, training a first diffusion model to produce a first synthetic histological slide image at a lower resolution using the translated RNA-Seq records and associated histological slides, training a second diffusion model to upscale lower resolution synthetic histological slide images produced by the first diffusion model to higher resolution synthetic histological slide images, obtaining a given RNA-Seq record, translating the given RNA-Seq record into the latent space, providing the latent representation of the given RNA-Seq record to the trained first diffusion model to generate a given lower resolution synthetic histological slide image, and providing the given lower resolution synthetic histological slide image to the trained second diffusion model to generate a given higher resolution synthetic histological slide im