Abstract: The invention provides for delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are delivery systems and tissues or organ which are targeted as sites for delivery. Also provided are vectors and vector systems some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity and to edit or modify a target site in a genomic locus of interest to alter or improve the status of a disease or a condition.

Abstract: Described herein are systems, methods, and compositions capable of targeting nucleic acids. Describe in certain exemplary embodiments herein are a class of small Cas proteins (Type II-D Cas proteins) and systems thereof. Also described in certain exemplary embodiments herein are methods of modifying target sequences using the class of small Cas proteins (Type II-D Cas proteins) and systems thereof described herein.

Abstract: Techniques are provided to form semiconductor devices that include through-gate structures (e.g., gate cut structures or conductive via structures) that have an airgap spacer between the structure and the adjacent gate electrode. In an example, a semiconductor device includes a gate structure around or otherwise on a semiconductor region (or channel region) that extends from a first source or drain region to a second source or drain region. A through-gate structure may extend in a third direction through an entire thickness of the gate structure and adjacent to the semiconductor region along the second direction. The through-gate structure may be a dielectric structure (e.g., a gate cut) or a conductive structure (e.g., a via). In either case, an airgap spacer exists between the through-gate structure and the gate structure.

Abstract: A hybrid powertrain and a vehicle are provided. The hybrid powertrain includes a first electrical machine, an engine, a main shaft, a clutch assembly, a second electrical machine, and a power battery. One end of the main shaft is connected to an output shaft of the engine, and another end of the main shaft is selectively connected to an electrical machine shaft of the first electrical machine through the clutch assembly. The first electrical machine is configured to selectively output power to a first wheel end through the clutch assembly. The second electrical machine includes a rotor and a stator, the rotor is configured to rotate relative to the stator, and the rotor is fixedly connected to the main shaft. The first electrical machine and the second electrical machine are connected to the power battery.

Abstract: A phase shifter includes first and second dielectric substrates opposite to each other and a plurality of phase shift units between the first and the second dielectric substrates; the phase shift unit includes first and second electrode layers and an adjustable dielectric layer between the first and the second electrode layers; orthographic projections of the first and second electrode layers on the first dielectric substrate at least partially overlap each other, and at least one accommodation cell of the phase shift unit is defined in a region where the orthographic projections of the first and second electrode layers on the first dielectric substrate overlap each other; the adjustable dielectric layer is in at least the accommodation cell; and cell gaps of the accommodation cells of at least a part of the plurality of phase shift units are different from each other.

Abstract: Techniques are provided to form semiconductor devices where portions of the gate structure (e.g., foot structures) adjacent to the subfins have been removed. A semiconductor device includes a gate structure around or otherwise on a semiconductor region. The gate structure includes a gate dielectric and a gate electrode. The gate structure may be interrupted, for example, between two transistors with a gate cut that extends through an entire thickness of the gate structure and includes dielectric material to electrically isolate the portions of the gate structure on either side of the gate cut. The gate cut includes dielectric lobe structures that extend outwards from the sidewalls of the gate cut. The lobe structures effectively replace foot structures of the gate structure between the gate cut and subfin portions of the semiconductor fins. Removing the gate foot structures contributes to the reduction of the parasitic capacitance in the semiconductor device.

Abstract: The embodiments disclosed herein utilized RNA targeting effectors to provide a robust CRISPR-based diagnostic with attomolar sensitivity. Embodiments disclosed herein can detect broth DNA and RNA with comparable levels of sensitivity and can differentiate targets from non-targets based on single base pair differences. Moreover, the embodiments disclosed herein can be prepared in freeze-dried format for convenient distribution and point-of-care (POC) applications. Such embodiments are useful in multiple scenarios in human health including, for example, viral detection, bacterial strain typing, sensitive genotyping, and detection of disease-associated cell free DNA.

Abstract: A frame for a pool that may be installed as a series of frame assemblies is disclosed. The frame assemblies may comprise a frame member, a sleeve comprising an end wall and a protrusion extending from the end wall, and a joint configured to receive the frame within the sleeve that fits within an opening of the joint, and receive a portion of the protrusion of the sleeve to lock the protrusion in place. The sleeve may comprise one or more ribs on an outer wall of the sleeve.

Abstract: Provided herein are compositions, systems, and methods for delivering cargo to a target cell. The compositions, systems, and methods comprise one or more polynucleotides encoding one or more LTR retroelement polypeptides for forming a delivery vesicle and one or more capture moieties for packaging a cargo within the delivery vesicle. The one or more LTR retroelement polypeptides for forming a delivery vesicle may comprise two or more of an LTR retroelement gag protein, a retroelement envelope protein, an LTR retroelement reverse transcriptase, or a combination thereof. The LTR retroelement polypeptide alone, the LTR retroelement envelope protein alone, or both the LTR retroelement-derived polypeptide and LTR retroelement envelope protein may be endogenous.

Abstract: Described herein are engineered paraneoplastic Ma protein (PNMA) capable of forming a capsid. In some embodiments, the engineered PNMA proteins comprise one or more modifications that enhance binding or loading of a cargo into the capsid, one or more modifications that modify cell-specificity of the capsid, one or more modifications that enhance intracellular delivery of the capsid, or a combination thereof. Also described herein are delivery systems comprising capsids comprising an engineered PNMA protein and a cargo.

Abstract: An R-tree index merging and updating method and apparatus based on a Hilbert curve, and a medium. The method comprises: S1, acquiring a first R-tree to be merged and a second R-tree into which the first R-tree is to be inserted; S2, querying a node for insertion, for each merged leaf node in a leaf node level of the first R-tree by means of a “top to bottom” hierarchical query algorithm; S3, inserting, according to an order of the Hilbert values, spatial objects included in each leaf node in the first R-tree into the node for insertion that is determined in S2, thereby merging the two R-trees; and S4, for the second R-tree after merging, updating information stored in each node level by level according to an order from a leaf node to a root node.

Abstract: The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered DNA or RNA-targeting systems comprising a novel DNA or RNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA.

Abstract: A computer implemented method includes receiving a start date and an end date specifying a future absence or away-from-work time period for a user, generating an away from office communication specifying the away-from-work time period, retrieving via the computer a list of collaborators based on frequency and duration of recent interactions with the user, receiving via the computer a selection of collaborators from the list, identifying meetings involving the user during the away-from-work time period, generating via the computer a list of user viewable items for the user to complete prior to the away-from-work time period, and generating via the computer a list of items for the user to attend to upon return from the away-from-work time period for presentation to the user upon return.

Abstract: A kitchen waste wastewater treatment equipment includes a reaction kettle, a discharging mechanism and a pressurizing mechanism. In the process of high-temperature and high-pressure thermal hydrolysis of wastewater in the present disclosure, the pressurizing disk in the pressurizing mechanism continuously moves downwards in a vacuum state in the reaction kettle, reducing the volume in the reaction kettle and thus increasing the pressure. The secondary pressurization of wastewater is achieved while using heating for high-temperature and high-pressure, and the high-temperature and high-pressure environment greatly improves the reaction rate of thermal hydrolysis. The thermal hydrolysis reaction also generates gas for further pressurization, which greatly improves the treatment efficiency of wastewater. Moreover, the pressurizing mechanism and the exhaust annular groove can separate the gas generated after thermal hydrolysis.

Abstract: A clutch assembly includes a first transmission member, a first clutch component, and a second clutch component. The first clutch component includes a first clutch portion and a second clutch portion, and the second clutch component includes a third clutch portion and a fourth clutch portion. The first clutch portion and the third clutch portion are connected to the first transmission member to enable the first transmission member to form a first end of the clutch assembly. The second clutch portion is selectively engaged with the first clutch portion to enable the second clutch portion to form a second end of the clutch assembly. The fourth clutch portion is selectively engaged with the third clutch portion to enable the fourth clutch portion to form a third end of the clutch assembly.

Abstract: The present disclosure relates generally to the field of delivery systems using contractile injection systems (CIS). Specifically disclosed are engineered extracellular CISs (eCISs) that can deliver non-natural protein payloads to non-natural target cells such as human cells. In addition, methods of using the engineered eCISs are also disclosed.

Abstract: This disclosure is directed to a targeted delivery vehicle that can deliver a cargo to a cell of interest. The targeted delivery vehicle has a fusogen and a targeting domain which are embedded in a lipid bilayer membrane that forms a vesicle, and a cargo within the vesicle. The disclosure is also directed to methods for targeted delivery of cargo using the targeted delivery vehicle described herein.

Abstract: Disclosed is a method for recommending real-time audios and/or videos, including acquiring description data of a plurality of real-time audios and/or videos; acquiring user data; determining at least one candidate real-time audio and/or video in the real-time audios and/or videos; acquiring audio and/or video data of the at least one candidate real-time audio and/or video upon initiation of the candidate real-time audio and/or video; acquiring content data of the at least one candidate real-time audio and/or video; acquiring a degree of association between the content data of the at least one candidate real-time audio and/or video and the user data; and displacing a target candidate real-time audio and/or video in the at least one candidate real-time audio and/or video in a user interface.