Osmosensing histidine kinases

Abstract

The present invention relates to histidine kinases, including osmosensing fungal histidine kinases. In particular, the present invention provides amino acid and nucleic acid sequences of fungal histidine kinases from organisms such as Candida (e.g., C. albicans) and Neurospora (e.g., N. crassa). The present invention further provides compositions and methods for the development of antifungal compounds.

Claims

1. A purified and isolated nucleic acid sequence encoding an osmosensing histidine kinase, wherein said sequence is selected from the group consisting of SEQ ID NO:1 and its fully complementary sequence.

2. The purified and isolated nucleic acid sequence of claim 1, wherein said nucleic acid sequence is from Neurospora.

3. A composition comprising the nucleic acid sequence of claim 1.

4. An expression vector containing the nucleic acid sequence of claim 1.

5. A host cell containing the vector of claim 4.

6. The host cell of claim 5, wherein said host cell is prokaryotic.

7. The host cell of claim 5, wherein said host cell is eukaryotic.

8. The host cell of claim 9, wherein said host cell is a fungal cell.

9. The host cell of claim 8, wherein said host cell is selected from the group consisting of Neurospora, Candida, Aspergillus, and Saccharomyces.

10. The host cell of claim 9, wherein said host cell comprises Candida albicans.

11. The host cell of claim 8, wherein said host cell comprises SEQ ID NO:1, such that said host cell expresses a fungal histidine kinase.

12. The host cell of claim 11, wherein said fungal histidine kinase is a Neurospora crassa histidine kinase.

13. The host cell of claim 11, wherein said fungal histidine kinase is a Candida albicans histidine kinase.

14. The host cell of claim 11, wherein said fungal histidine kinase is a Saccharomyces histidine kinase.

15. A purified and isolated nucleic acid sequence comprising SEQ ID NO:17, wherein said nucleic acid sequence encodes an osmosensing histidine kinase.