Abstract: The present invention provides for systems and methods for acquiring acoustic information from a resonating body. Specifically, a focused light beam is emitted from an emitter module onto an impedance matching reflector. The impedance matching reflector has similar wave propagation characteristics to the resonating body, so as to capture a mechanical vibrations thereof. The impedance matching reflector reflects the focused light beam as it vibrates, and the vibrations are captured by a receiver module through the reflected light beam, which is converted to an input signal. The input signal may be further processed and converted to an audio output signal, as an accurate representation of the acoustic information emanating from within the resonating body.

Abstract: A method for evaluating atomic vacancies in a silicon wafer surface layer includes: element formation in which a pair of comb-shaped electrodes are formed on the same surface of a silicon sample over piezoelectric thin films; detection during which the sample is cooled and an ultrasonic pulse generated from one electrode while an external magnetic field is applied, the ultrasonic pulse being propagated through the sample surface and received by the other electrode, and a phase difference being detected between the ultrasonic pulse generated by the one electrode and the ultrasonic pulse received by the other electrode; and evaluation during which the sample surface elastic constant is determined on the basis of the phase difference, and the atomic vacancies in the sample surface are evaluated on the basis of changes in the elastic constant according to temperature or changes in the elastic constant according to the magnetic field intensity.

Abstract: An ultrasound probe assembly includes a transducer configured to transmit and receive ultrasound signals in relation to a structure, and a delay line coupled to the transducer. The delay line is configured to change shapes between an uncompressed state and a compressed state. In at least one embodiment, the changing shape of the delay line changes a shape of the ultrasound signals.

Abstract: The invention describes the process by which a standard mixture of organic compounds are spiked and retained onto a composite sorbent matrix for the controlled generation of a standard in fluid above the spiked matrix either in gas (headspace) or aqueous phase which is contained in portable vials. The novelty of the aforementioned composite matrix stems from the combination of an immobilizing liquid phase such as silicone oil or a polyacrylonitrile solution, and solid, porous particles such as polystyrene-co-divinylbenzene (PS-DVB) or Hydrophilic/Lipophilic Balance (HLB) particles to strongly retain the spiked standards facilitating high capacity which translates into prolonged use as a source of calibrant. These novel composite mixtures exhibit sorptive capabilities greater than the sum of their individual components producing a standard analyte generator far superior to those described in the prior art.

Abstract: A method for controlling the flow of liquid in a high performance liquid chromatography apparatus. The method includes operating a pump, measuring the liquid pressure downstream of the pump, measuring the liquid flow rate downstream of the pump, and controlling the operation of the pump. In the method, it is automatically determined whether the pump is controlled to achieve a desired pressure or controlled to achieve a desired flow rate. Fuzzy logic can be applied in the method to determine the switch between the control modes.

Abstract: Disclosed herein are compounds and methods for identifying and quantifying a metabolite or breakdown product of PEG. A sample may be assayed for PEG metabolites or breakdown products using liquid chromatography combined with mass spectrometry. Derivatization of the PEG metabolites or breakdown products within the sample with pentaflurobenzoyl chloride in conjunction with negative chemical ionization mode liquid chromatography optimizes the assay.

Abstract: In order to achieve high sensitivity without an increase in device complexity or cost, a far-ultraviolet absorbance detection device for liquid chromatography is provided with: an optical system including a light source that emits light including far-ultraviolet light, a diffraction grating for dispersing the light emitted from the light source, a flow cell through which a liquid is passed, a slit for selecting a predetermined wavelength of +1 order light diffracted by the diffraction grating and causing the light to enter the flow cell, a first photodetector for detecting the light transmitted by the flow cell, and a second photodetector for detecting light other than the +1 order light diffracted by the diffraction grating; a mechanism for evacuating or substituting the optical system with nitrogen gas; and a computation unit that calculates absorbance from an output signal from the first photodetector and an output signal from the second photodetector. The second photodetector is fixedly disposed.

Abstract: Rose Bengal for detecting a presence of chemical warfare agents. A method of detecting presence of a chemical warfare agent and includes applying a quinoid form of Rose Bengal to a substrate. When the substrate is exposed to the chemical warfare agent, a lactone form of Rose Bengal is spectrally observed because presence of the chemical warfare agent converts the quinoid form to the lactone form of Rose Bengal.

Abstract: Compositions that include bis(glyoxime)-transition metal complexes bound to metal oxide solid supports are provided. In some embodiments the compositions include nickel dimethylglyoxime and the metal oxide supports can be alumina or silica. These compositions can be used to make a colorimetric moisture-indicating sensor that changes visible reflection spectrum quantitatively and reversibly according to the amount of moisture in contact with the sensor. Also provided is a method of detecting moisture using the provided compositions.

Abstract: A gas processing device includes a sample inlet defining a gas flow path into a housing, and a pump and a noise dampening device disposed in the housing. The noise dampening device includes an inlet chamber, an outlet chamber, and an elastomeric membrane interposed between and fluidly isolating the inlet chamber and the outlet chamber. The inlet chamber is interposed between the sample inlet and the pump inlet, and the outlet chamber communicates with the pump outlet.

Abstract: A replaceable spectroscopic detector used in volatile organic compounds testing devices, such as a portable breath testing device for roadside drug testing or a testing device for any air handling systems, such as those used for indoor agriculture, which reversibly sorb compounds and prepare a concentrated sample in a single gas cell configured for performing spectroscopy of the contents within the cell. The disclosed invention comprising; one or more check valves, a source of electromagnetic radiation and an electromagnetic receiver that releasably connects to a volatile organic compounds testing device such that it is replaceable and is configured for performing spectroscopy and passing exhaled breath and/or airflow through a disposable and replaceable reversibly engaging cartridge comprising a reversible sorbent and a gas cell configured for spectroscopy.

Abstract: Various environmental systems are disclosed. In accordance with one of the systems, a first and second valve are provided. The valves provide a first air flow path from an air pump to a photo-ionization detector is provided through a pre-filter. The valves also provide a second air flow path from the air pump to the photo-ionization detector so that the pre-filter is isolated from its environmental surroundings. Various other arrangements of environmental monitoring systems with one or more pre-filters are also disclosed.

Abstract: A system for detecting a gas released during production of methamphetamine in a residential building includes a sensor. The sensor is attachable to a part of the residential building. The sensor outputs a signal in response to detection of a concentration of the gas. The system includes a communication link.

Abstract: Methods, system and device are provided for detection and quantitative and qualitative analysis of components in a gaseous mixture distinguished by high selectivity and high resolution. The influence of individual gases may be distinguished through detection of changes associated with a sensor's sensitive layer that interacts with the components of the gaseous mixture. Through periodic variations of parameters or conditions of the sensor, the characteristics of the gas components may be derived. For example, the concentration of a gas or multiple gases in a mixture may be determined with a high degree of accuracy. Non-invasive detection of biological off-gases may be implemented. Other uses abound.

Abstract: The invention relates to an electrochemical method for determining the DOC and/or TOC and/or IC content in a water sample by means of anodic oxidation on electrodes in a closed electrolysis cell, wherein the carbon is oxidized at least partially to carbon dioxide (CO2). The carbon dioxide thereby formed is collected in a closed gas space, wherein, in the range of the essentially linear increase in the CO2 content in the gas space, the rate at which this increase takes place is determined and used in an analyzer unit to determine the DOC and/or TOC and/or IC content.

Abstract: A system and method is provided for enhancing hydrocarbon production. The method and system involve geochemistry analysis and include multiply substituted isotopologue and position specific isotope geochemistry. The method and system involve using clumped isotope and position-specific isotope signatures to enhance reservoir surveillance operations.

Abstract: The invention relates to a sensor unit for determining properties of a lubricant; for example, a lubricating grease in a rolling bearing. The invention further relates to a machine element having the sensor unit according to the invention and to a machine assembly having the machine element according to the invention. The sensor unit according to the invention first of all includes a sensor housing having a window which can be aligned to the lubricant. A transmitter for emitting electromagnetic radiation through the window and a receiver for receiving electromagnetic radiation which passes through the window are located in the sensor housing. Evaluating electronics arranged in the sensor housing are electrically connected at least to the receiver. The evaluating unit is designed to generate at least one digital sensor signal. The sensor unit further includes an electrical connection, which is led through the sensor housing and electrically connected to the evaluating electronics in the sensor housing.

Abstract: This disclosure describes a level indicator assembly for measuring the level of solids in a vessel. The level indicator may desirably comprise a solid shaft extending into a vessel. The shaft may have paddle connected to an interior end of the shaft and an external sensor attached to an exterior end of the shaft. A support structure may engage the sensor to prevent at least a portion of the sensor from moving. By keeping a portion of the sensor stationary, the sensor may measure the change in torsional force exerted on the shaft. By locating the sensor external to the vessel, operators may remove and replace sensors without accessing either the vessel interior or the portions of the level indicator assembly that extend into the vessel.

Abstract: Embodiments of the disclosure are directed to a device for molecule sensing. In some embodiments, the device includes a first electrode separated from a second electrode by a dielectric layer. The first electrode comprises a large area electrode and the second electrode comprises a small area electrode. At least one opening (e.g., trench) cut or otherwise created into the dielectric layer exposes a tunnel junction therebetween whereby target molecules in solution can bind across the tunnel junction.

Abstract: A sensor storage container includes: a lid configured to cover in an openable and closable manner an opening of an open-topped main body case that stores an elongated plate-like sensor lying flat; and a sensor ejecting tool configured to take the sensor out of the main body case. The sensor ejecting tool is elongated, and includes two ends, one end being linked to one of end sides of an opening portion of the main body case, and the other end being rotatable in a vertical direction between a bottom portion of the main body case and a position above the opening portion. A sensor adhesive portion at the other end of the sensor ejecting tool is provided with an adhesive face on a side of the sensor adhesive portion facing a bottom face of the main body case.

Abstract: A container device for storing, singulating and dispensing rigid and semi-rigid strips such as diagnostic test strips. A plurality of strips is contained and protected within a transparent container having an internal capture element for capturing and positioning a single strip adjacent a narrow normally-closed dispensing aperture. The aperture is exposed by rotating a spring-loaded container top cap to an open position. A detachable container base cap contains a desiccant and is replaceable. An optional stand stores the device in an upright position, and incorporates a tool to facilitate removal of the base cap for replenishing the container's contents or replacing the desiccant. A radio frequency identification (RFID) tag can be incorporated for electronic inventory control. The device can also be used as an integrated test strip container/single strip dispensing means for an automated analyte meter module.

Abstract: A microfluidic device for separating liquid from the same liquid containing deformable particles without external sources of energy. The device includes: at least one transport channel (1) using various fluid-hydrodynamic principles in order to increase the amount of separated/obtained fluid; and at least one separating area (2) which diverts part of the fluid circulating through the transport channel towards at least one collection channel (3) or reaction chamber. The separating area (2) can include at least one array of pillars, the configuration of which maximizes the amount of fluid extracted at once. The depth and width of the collection channel (3) are adaptable according to the requirements of the test to be implemented. A filter is provided for separating plasma from a drop of blood in a highly efficient manner, which can be used in point-of-care testing systems.

Abstract: A variety of applications, systems, methods and constructs are implemented for use in connection with screening of ion-channel modulators. Consistent with one such system, drug candidates are screened to identify their effects on cell membrane ion channels and pumps. The system includes screening cells having light responsive membrane ion switches, voltage-gated ion switches and fluorescence producing voltage sensors. A chemical delivery device introduces the drug candidates to be screened. An optical delivery device activates the light responsive ion switches. An optical sensor monitors fluorescence produced by the voltage sensors. A processor processes data received from the optical sensor. A memory stores the data received from the optical sensor.

Abstract: The invention provides novel methods of determining or comparing potency of a test interferon relative to rSIFN-co (an interferon having therapeutic effects on solid tumors); methods of establishing substantial equivalence between a test interferon and rSIFN-co; methods for down-regulation of expression of Wnt-related receptors or co-receptors, such as LRP6/FZD6; down-regulation of expression of Wnt-related target genes, such as, Axin2, CD24, Survivin and/or ID2; inhibition of beta-catenin/TCF transcriptional activities; suppression of expression of beta-catenin; up-regulation of tumor suppressor genes, such as DKK-3, BATF2 and/or KLF4; inhibition of tumor cell viability in vitro; inhibition of tumor growth and metastases in vivo; inhibition of tumor cell migration, pseudopod formation, and colony formation in vitro; as well as methods for determining potency of a test interferon, kits for determination of such methods, and an interferon or an interferon substitute having said activities.

Abstract: Disclosed is a method for selecting a cancer treatment regimen for a subject.

Abstract: The invention features a method of identifying a therapeutically active compound that inhibits apoptotic compensatory signaling complex (ACSC)-induced cell proliferation.

Abstract: The present invention provides methods of determining cell sensitivity to a therapeutic agent.

Abstract: Assays that can measure the effect of proteasome inhibitors on target cells in a biological sample are provided. The assays include evaluation of the effects of proteasome inhibitors on proteasome activity in cells in a biological sample.

Abstract: A blood test device is configured to measure a blood count and an electrolyte in a blood sample. The blood test device includes: a sample placement section in which a blood sample containing an anticoagulant containing a salt of ethylenediaminetetraacetic acid (EDTA) other than sodium salt, potassium salt, or calcium salt of EDTA is placed; and a measurement section by which measurement of a blood count and an electrolyte is carried out for the blood sample placed in the sample placement section.

Abstract: This invention relates to a method for analyzing gas samples, particularly with the aid of lanthanide(III) ions and ligands. The invention relates also to a kit for analyzing gas samples utilizing the properties of lanthanide(III) chelates.

Abstract: The present invention provides a urine test device incorporating a urinary catheter attachment device. This urine test device may be used in an ER environment or the like to simultaneously test a catheterized (or uncatheterized) patient for pregnancy, alcohol, a variety of illicit and legal drugs, blood in the urine, urine glucose, disease, point of care concerns, and/or the like. The urine test device is selectively attached to a patient's urinary catheter (or the associated urinary bag or tubing) to conveniently extract a urine sample for testing on site. Alternatively, a urine sample can be introduced into the urine test device via a more conventional approach, such as voluntarily by the patient, or by syringe by a medical professional. Appropriate attachments are provided for these purposes.

Abstract: Methods of detecting a target analyte in a sample are provided. Aspects of the method include: (a) contacting the sample with (i) a first capture agent that specifically binds the target analyte and (ii) a reporter complex under conditions sufficient to produce a sandwich complex; (b) separating the sandwich complex from the sample; and (c) releasing a detectable tag from the sandwich complex. The reporter complex may include a first specific binding member linked to a second capture agent that specifically binds the target analyte, and a second specific binding member specifically bound to the first specific binding member. In some cases, the second specific binding member is linked to a detectable tag. The releasing step may be achieved using a displacement binding member that is complementary to the first or second specific binding member. Also provided are compositions, systems and kits for practicing the subject methods.

Abstract: Provided are methods for detecting a target molecule or particle suspected to be present in a sample, comprising (a) contacting the sample with (i) a fusion molecule comprising a ligand capable of binding to the target molecule or particle and a binding domain, and (ii) a polymer scaffold comprising at least one binding motif to which the binding domain is capable of binding, under conditions that allow the target molecule or particle to bind to the ligand and the binding domain to bind to the binding motif; (b) loading the polymer into a device comprising a pore comprising an opening in a structure that separates an interior space of the device into two volumes, and configuring the device to pass the polymer through the pore from one volume to the other volume, wherein the device further comprises a sensor configured to identify objects passing through the pore; and (c) determining, with the sensor, whether the fusion molecule or particle bound to the binding motif is bound to the target molecule or particle

Abstract: Provide is a stable metallic nanostructure that causes no aggregation when surface-modified with biomolecule-reactive functional molecules. 30 to 90% of the surface of the metallic nanostructure is covered with at least one or more types of colloid-stabilizing functional molecules. Furthermore, the metallic nanostructure is covered with one or more types of biologically functional molecules.

Abstract: The present disclosure provides an improved field effect transistor and device that can be used to sense and characterize a variety of materials. The field effect transistor and/or device including the transistor may be used for a variety of applications, including genome sequencing, protein sequencing, biomolecular sequencing, and detection of ions, molecules, chemicals, biomolecules, metal atoms, polymers, nanoparticles and the like.

Abstract: An implantable diagnostic device in accordance with the present disclosure provides various benefits such as a compact size thereby allowing implanting of the device inside animate objects; low cost due to incorporation of inexpensive detection circuitry and the use of conventional IC fabrication techniques; re-usability by heating thereby allowing multiple diagnostic tests to be performed without discarding the device; and a configuration that allows performing of simultaneous and/or sequential diagnostic tests for detecting one or more similar or dissimilar target molecules concurrently or at different times.

Abstract: A test device for use with a liquid solution includes a housing that houses a plurality of sorbent strips. At least one strip has a location for receiving the solution. The housing defines at least an opening in said housing wherein said opening is aligned with the location for receiving the solution. The device includes a sealed reservoir filled with the liquid solution, the sealed reservoir being disposed in alignment with the opening and coupled to the housing. The device includes an opener coupled to the housing. The opener is arranged to rotate from a first position, in which the opener is not in contact with the sealed reservoir, to a second position, in which the opener causes the sealed reservoir to effect a release of the solution from the reservoir and into the opening and onto the sorbent strip housed within the housing.

Abstract: A microreactor array system includes an array of microreactors, and a sealing membrane having a first surface and an opposite second surface, the sealing membrane configured to movably seal the array of microreactors. The microreactor array system further includes a reagent gap providing a fluid path between the array of microreactors and the second surface of the sealing membrane when the second surface of the sealing membrane is in spaced relationship with the array of microreactors. The microreactor array system further includes an injector for delivering a reagent into the reagent gap, an applicator for directing a working fluid against the first surface of the sealing membrane, and a detector.

Abstract: An immunosensing device includes a hub mountable to a device for fluid collection, such as a syringe, sample collection container, or vacuum collection container, and a biosensor mounted to the hub. The biosensor includes a biosensor active area in fluid communication with a fluid passage within the hub. The biosensor active area includes nanoelements functionalized with one or more antibodies or antigen binding fragments thereof for contact with a fluid in the fluid passage. The biosensor active area can include multiple regions each including nanoelements functionalized with a different antibody or antigen binding fragment thereof to detect multiple biomarkers. The biosensor, separately or attached to the hub, can be inserted in a biomarker reader for detection of a biomarker in the fluid.

Abstract: A process for the identification of compounds with a molecular weight in the range 100 to 750 which inhibit the binding of the first and/or second bromodomains of human BRD-2 to 4 to acetylated lysine residues of their physiological partner proteins which comprises selecting those compounds which are able to: a) form a hydrogen bonding interaction in which the compound accepts a hydrogen bond from the sidechain NH2 group of the asparagine residue found at: BRD-2 BRD-2 BRD-3 BRD-3 BRD-4 BRD-4 BD1 BD2 BD1 BD2 BD1 BD2 ASN156 ASN429 ASN116 ASN391 ASN140 ASN433 or b) accept a water-mediated hydrogen bond in which the compound accepts a hydrogen bond from a water that is itself hydrogen-bonded to the sidechain hydroxyl of the tyrosine residue found at BRD-2 BRD-2 BRD-3 BRD-3 BRD-4 BRD-4 BD1 BD2 BD1 BD2 BD1 BD2 TYR113 TYR386 TYR73 TYR348 TYR97 TYR390 and c) which are also able to form a Van der Waals interaction with a lipophi

Abstract: A composition for classifying a human pediatric subject having adolescent idiopathic scoliosis (AIS), comprising (a) a cell sample of the subject; and (b) a known melatonin-signaling pathway agonist, wherein said melatonin signaling pathway in said cell sample has been artificially induced by said melatonin-signaling pathway agonist, wherein the cell sample is selected from the group consisting of blood cell sample, osteoblast cell sample, osteoclast cell sample and myoblast cell sample, and whereby the composition enables the detection of the presence or absence of at least one impairment in the melatonin-signaling pathway in cells from said cell sample and thereby enables the classification of subject.

Abstract: Specific DNA sequences for binding various foodborne and waterborne pathogens and biotoxins are described. Each of these sequences can function in varying assay and sensor formats with varying degrees of success.

Abstract: According to aspects illustrated herein, there are provided a sensor chip suitable for serological detection of Streptococcus pneumoniae, a method for detecting serotypes of Streptococcus pneumoniae using the sensor chip, a detection system that includes the sensor chip, and a method for detecting serotypes of Streptococcus pneumoniae using the detection system described herein.

Abstract: The present disclosure highlights the relationship between extracellular mitochondrial components, optionally in combination with the secreted phospholipase A2-IIA and/or an auto-antibody, and in vivo as well as in vitro and inflammatory reactions/conditions, especially those released as a result of the degradation of a platelet. The present disclosure provides methods for determining the presence of inflammatory mediators, for limiting inflammatory reactions/conditions, for the diagnosis inflammatory reactions/conditions, for screening therapeutics for the treatment and/or the alleviation of symptoms of inflammatory reactions/conditions based on the detection or modulation of the level of these extracellular mitochondrial components.

Abstract: The human HLA-A2:1g dimer molecule is a recombinant protein comprising a mouse IgG antibody fused with two human MHC Class I HLA-A2 molecules. Any peptide (usually 8-10 amino acids in length) can be loaded into the peptide-binding groove of the two HLA molecules, for example, by incubating a mixture of the dimer and peptide solution overnight. The resulting N peptide-specific HLA-A2:1g dimer mixture can then be added to PBMCs from peripheral blood samples in order to detect CD8 T lymphocytes which express T cell receptors that are capable of specifically interacting with and binding to the peptide HLA-A2:1g dimer molecules. The presence of such specific binding activity and interactions can then be detected by additional staining with fluorescence-conjugated antibodies.

Abstract: This present invention relates to a method for selecting an antigen-specific hybridomas comprising catching a monoclonal antibody secreted from a hybridoma by using a plasma cell's cell-surface expression molecular on the surface of the hybridoma, providing an antigen with a label to react with the hybridoma, and selecting the hybridoma expressing the label to obtain an antigen-specific hybridoma. In other words, the hybridoma can express the label because of the monoclonal antibody combining with the antigen. Accordingly, the method of this present invention can quickly select the antigen-specific hybridoma.

Abstract: The present invention belongs to the field of compliance markers and marker vaccines which allow for the differentiation between infected and vaccinated individuals. In particular, it relates to a method of determining whether an individual has received an immunogenic composition comprising a recombinant protein produced by a baculovirus expression system in cultured insect cells.

Abstract: A method of diagnosing a subset of Epstein Barr Virus, Myalgic Encephalomyelitis Chronic Fatigue Syndrome (ME/CFS) patients through a multi-prong clinical/serological analysis is provided wherein Epstein Barr Virus Abortive Lytic Replication (EBV) is determined by detecting the presence of BMRF1 in serum antibody assays. A method of treating patients diagnosed with Epstein Barr Virus Abortive Lytic Replication (EBV), Myalgic Encephalomyelitis Chronic Fatigue Syndrome (ME/CFS) with specific antiviral nucleosides is also provided, to alleviate the condition.

Abstract: The present disclosure relates to monoclonal anti-LINE-1 ORF2 protein endonuclease domain antibody, and fragments and derivatives that bind the LINE-1 ORF2 protein endonuclease domain and inhibit its activity.

Abstract: The present disclosure provides high-performance immunoreagents for use in a variety of immunologic assays and other related techniques. The immunoreagents comprise a primary antibody and a bridging antigen, wherein the bridging antigen is recognized by a detectable secondary antibody with high affinity. Also provided are compositions comprising panels of immunoreagents specific for multiple different target antigens and compositions comprising pairs of primary immunoreagents and their complementary detectable secondary antibodies. The paired primary immunoreagents and secondary antibodies are useful in a variety of immunologic assays, particularly in highly multiplexed assays, where the structure of the bridging antigen is varied in tandem with variation in the detectable secondary antibody, such that a multiplicity of immunoreagents are provided that are capable of simultaneously detecting a multiplicity of target antigens in a single assay.