Abstract: An installation structure for grease sensor includes a housing having a through-hole. A resilient grommet is installed in the through-hole. The resilient grommet has an installation hole. A probe of the grease sensor extends from a base and is inserted into said installation hole such that the end of the probe extends into the interior of the housing to contact the grease. The inner surface of the resilient grommet is resiliently pressed against the outer surface of the probe to form a tightly sealing interface. This kind of installation structure allows effective grease sealing, and convenient and stable installation.

Abstract: A multi-dimensional data acquisition and analysis system for placenta tissue is provided, including a preparation area, a sampling area, a tension cable, a mobile platform, a tray, a mobile cabin door, a cabin door motor, a motor, a motor controller, binocular vision cameras, a laser point cloud detector, a hyperspectral camera, an ultrasonic ranging, a lifting motor, a lifting guide rail, ring light sources, an infrared sensor, a two-dimensional code area and a two-dimensional code identification camera. The technical scheme of the disclosure is adopted to solve the problem of lack of effective technical means for placenta detection at present.

Abstract: A lipid bimolecular membrane substrate 1A includes: a substrate 12 in which a microwell 20 opened on one surface is formed; a lipid bimolecular membrane 30 disposed on the substrate 12 so as to cover an opening of the microwell 20; and a sealing liquid 22 disposed between the substrate 12 and the lipid bimolecular membrane 30. The sealing liquid 22 contains an ionic liquid.

Abstract: Disclosed are a method for determining a platelet concentration of a blood sample, a hematology system and a storage medium.

Abstract: In certain embodiments, the invention stems from the discovery that analysis of population distribution curves of metabolite levels in blood can be used to facilitate predicting risk of autism spectrum disorder (ASD) and/or to differentiate between ASD and non-ASD developmental delay (DD) in a subject. In certain aspects, information from assessment of the presence, absence, and/or direction (upper or lower) of a tail effect in a metabolite distribution curve is utilized to predict risk of ASD and/or to differentiate between ASD and DD.

Abstract: A method of automatically tracking urine in a toilet includes creating a background image of an interior of a toilet bowl using an image sensor, wherein the background image shows the interior of the toilet bowl. The method also includes acquiring an image of the interior of the toilet bowl using the image sensor, wherein the acquired image shows urine and a collecting and tracking member in the interior of the toilet bowl. The method further includes producing a difference image that shows a difference between the acquired image and the background image, and processing the difference image to identify a location of the urine and the collecting and tracking member, and moving the collecting and tracking member to the identified location of the urine.

Abstract: A breath capture and analysis system includes a breath inlet for capturing a user's breath. The breath is drawn into the system and retained for analysis. The system includes a membrane that allows volatile organic compounds contained in the breath to pass therethrough. The volatile organic compounds pass into a vacuum chamber that includes a residual gas analyzer that is configured to analyze the volatile organic compounds. The analyzed breath is then vacated from the system.

Abstract: Embodiments include breath intoxicant monitoring systems and methods of operating a breath intoxicant monitoring system. In an embodiment, a breath intoxicant monitoring system, includes a first detection element, a second detection element, and a control module. The first detection element can be operable to detect a level of an intoxicant in a user's breath and to provide a continuous signal indicating the level of intoxicant. The second detection element can be operable to detect a level of the intoxicant. The second detection element includes a different type of element than the first detection element. The second detection element can have greater accuracy than the first detection element. The control module to selectively operate the second detection element based on the signal received from the first detection element, and to indicate whether the level of intoxicant in the user's breath exceeds a threshold. Other embodiments are also included herein.

Abstract: Provided is a method for determining an effect of a potential active agent on a sample comprising a co-culture comprising a target cellular object, TCO, and one or more further cell types, FCT, wherein the TCO has been labelled with a flourescent live-cell marker having a first emission/excitation profile, and with a luminescent cell-death marker, and optionally the FCT has been labelled with a fluorescent live-cell marker having a second emission/excitation profile different from the first emission/excitation profile, comprising capturing, during an acquisition event using a microscope, a first fluorescent image from the fluorescent live-cell marker having the first emission/excitation profile, and optionally a second fluorescent image from the fluorescent live-cell marker having the second emission/excitation profile, and a luminescence measurement of the sample, and comparing fluorescent images and luminescent measurements between sample contacted with the potential active agent and sample not contacted wi

Abstract: Combined treatment for cancer is provided. Accordingly, there is provided a method of selecting or determining therapeutic efficacy of a combination of agents for the treatment of cancer in a subject in need thereof, the method comprising: (i) culturing a cancerous tissue of the subject in an ex-vivo organ culture (EVOC) in the presence of a combination of an anti-cancer agent and an additional agent, said additional agent is inhibiting expression and/or activity of a target conferring innate resistance to said anti-cancer agent or increasing expression and/or activity of a target conferring innate sensitivity to said anti-cancer agent; and (ii) determining an anti-cancer effect of the combination on the tissue, wherein responsiveness of the tissue to the combination indicates the combination is efficacious for the treatment of the cancer in the subject.

Abstract: The present invention provides novel processes, compositions, and methods for analyzing or assaying the potency and/or functionality of tumor infiltrating lymphocyte (TIL) products for use in therapy, including human cancer therapy, and analyzing or assaying the potency and/or functionality of other polyclonal products, such as marrow infiltrating lymphocyte (MIL) and peripheral blood lymphocyte (PBL) products. Compositions, methods, and kits for preparing and treating cancer using TIL, MIL, and PBL products are also provided.

Abstract: The present disclosure provides methods, compositions, and devices for making and using three-dimensional biological tissues that accurately mimic native physiology, architecture, and other properties of native tissues for use in, among other applications, drug testing, tissue repair and/or treatment, and regenerative medicine.

Abstract: The present disclosure provides methods, compositions, and devices for making and using three-dimensional biological tissues that accurately mimic native physiology, architecture, and other properties of native tissues for use in, among other applications, drug testing, tissue repair and/or treatment, and regenerative medicine.

Abstract: A system is provided for developing a predictive immunity profile on the basis of the quantitative analysis of one or more samples from an individual. The system comprises: a device configured to perform quantitative analysis of the interaction between one or more target species and one or more probes in solution on a fluid sample to provided quantitative analysis data; a data store storing: personal data relating to at least one individual; at least one model of target/probe interaction; processing circuitry configured to access the data store and identify and retrieve data relevant to the sample; receive quantitative analysis data of the sample from the device; perform analysis to fit the model to the received quantitative analysis data; extrapolate, through the model, to create a predictive immunity profile for the individual, and update the data store with the quantitative analysis data.

Abstract: A centrifuge for cleaning a reaction vessel unit, having a rotor for holding at least one reaction vessel unit with its opening(s) directed outwardly, a motor for rotating the rotor around a rotation axis, a housing having a substantially cylindrical inner surface, wherein a drain is provided for discharging fluid expelled from the reaction vessel unit, wherein a gap is provided between the inner surface and the rotor so that by rotating the rotor a wind is generated which drives the expelled fluid on the inner surface to the drain wherein an aspiration pump is connected to the drain for discharging fluid.

Abstract: The present disclosure provides devices, kits, and methods for focusing/enriching and/or separating/sorting submicron size particles, including biological entities such as exosomes and other submicron size extracellular vesicles. Devices, kits, and methods of the present disclosure utilize ferrohydrodynamic manipulation to focus populations of submicron particles into a stream for enrichment and/or further sort various sub-populations of submicron particles based on size differences.

Abstract: The invention provides novel compounds, reagents, systems, and methods for detecting a metabolite related to a NRTI in a biological sample and use thereof in monitoring adherence to pre-exposure prophylaxis or anti-retroviral treatment. Such reagents comprise NRTI derivatives, analogs, NRTI derivatives conjugates, along with antibodies directed to same, which are useful for antibody-based methods, such as a lateral flow immunoassay and other point of care devices.

Abstract: The present invention relates to diagnostic test and technology.

Abstract: Microfluidic devices and methods for assaying molecules in aqueous droplets are provided. The methods include the steps of loading an aqueous sample onto a microfluidic device, dispensing one or more sample droplets into an oil filled droplet operations gap of the microfluidic device and optionally diluting the sample droplet, and transporting a sample droplet to a sensor zone and initiating the assay.

Abstract: The present disclosure relates to methods for determining the presence of or the amount of an analyte in a sample and to a slide for use in the method.

Abstract: Disclosed are various embodiments of a device comprising a synthetic polymeric substrate having a high quality finish upper surface, the upper surface having at least a bilayer coating comprising a first, reflective layer and a second, transparent layer. Also disclosed are kits containing embodiments of the disclosed device and detectable particles. Also disclosed are various embodiments of a method of using the disclosed device and various embodiments of a method of using the disclosed kit.

Abstract: The invention provides compositions and methods for detecting thyroid hormone blocking immunoglobulin (TBI). The invention's methods are sensitive and specific for TBI, and may be used for the dual detection of both TBI and TSI. The invention's compositions and methods are useful for the diagnosis of diseases that are associated with the presence of TBI and/or TSI, for monitoring the progress of disease and/or treatment regimens, therapeutics, vaccines, etc., and for assisting clinicians in making treatment decisions.

Abstract: Provided herein is a tubular protein capture device including a continuous tubular body having an inner surface and an outer surface and methods of using thereof for cell clarification purposes. A protein selected from protein A, protein G, protein L or combination thereof is attached to the inner surface of the tubular protein capture device described herein. The devices and methods provided herein are useful for improving the efficiency and/or capacity of cell clarification to separate proteins. In some embodiments, the continuous tubular body of the present technology has a helical shape. In other embodiments, the continuous tubular body of the present technology includes a plurality of tubes aligned within a hollow enclosure.

Abstract: Combinations of Trypanosoma cruzi polypeptides, fusion protein formed therefrom, and compositions and methods of use thereof for improved detection of antibodies against T. cruzi are disclosed. Preferred polypeptide combinations include two or more polypeptides selected from Table 1, or a variant or fragment thereof. In particularly preferred embodiments, the polypeptide combinations include the two polypeptide as paired in Table 2 or Table 3, or variants or fragments thereof. Preferably, the one, or more preferably both, of the polypeptides are antigenic to T. cruzi antibodies. The polypeptide combination can unfused or fused for form fusion proteins. Methods of using the disclosed compositions, including methods of detecting anti-T. cruzi antibodies, diagnosing T. cruzi infections, and monitoring disease status and treatment efficacy are also provided.

Abstract: Provided herein are methods for detecting an antigen or for detecting expression of a chimeric antigen receptor (CAR). The methods include obtaining a sample from a subject, contacting the sample with a fusion protein comprising a reporter fused to a single chain antibody specific to the antigen or fused to an extracellular domain of an antigen targeted by the CAR or fused to Protein L and assaying the activity of the reporter, wherein presence of reporter activity or increase in reporter activity relative to a reference value is indicative of presence of the antigen or presence of the expression of the chimeric antigen receptor in the sample.

Abstract: Methods of assessing or evaluating functional properties of T cell therapeutic products are described herein.

Abstract: The invention relates to methods for determining the presence and/or amount of flavivirus-reactive complement-fixing antibodies in a sample from a subject. Further, the invention is related to methods for the concomitant determination of the presence and/or amount of complement-fixing antibodies reactive to different flaviviruses in a sample from a subject. Moreover, the invention is related to in vitro methods for diagnosing a flavivirus infection in a subject. In addition, the present invention also provides kits for carrying out the methods.

Abstract: The present disclosure relates to a nanostructure for detecting viruses including an amphipathic polymer, and a diagnostic platform using the same, wherein the nanostructure is capable of specifically detecting viruses through silica-based nanoparticles with excellent stability and high dispersion and a biocompatible amphipathic polymer, such that it is possible to develop a diagnostic platform with high sensitivity through binding and agglomeration of the nanostructure and viruses and enable rapid and accurate diagnosis of a target virus.

Abstract: The disclosure relates to use of the HE4/HE4a marker(s) together with one or more other markers to assess ovarian cancers in a patient.

Abstract: The present disclosure provides the use of BAZ1B_K426hy in the preparation of a product for tumor detection and belongs to the field of biotechnology. The present disclosure further provides a group of immunogenic polypeptides, including polypeptide A and polypeptide B. An anti-BAZ1B_K426hy polyclonal antibody is prepared by conducting mixed immunization on an animal with the immunogenic polypeptide. The polyclonal antibody can specifically recognize an endogenous protein BAZ1B_K426hy by enzyme-linked immunosorbent assay (ELISA)/Dot blot/Western blot, which is used for preparation of detection products for tumors and Williams syndrome.

Abstract: The invention provides a method for quantifying the number of target entities that were successfully encapsulated into a microfluidic compartment such as a microfluidic droplet. The invention is predicated upon a continuous quantifiable detectable signal corresponding to the quantity of entities encapsulated in a microfluidic compartment. The present invention is useful in the context of high throughput microfluidic screening approaches which require thorough signal normalization in order to reliably improve signal to noise ratio and to reliably identify screening hits.

Abstract: The present disclosure provides a method of identifying a polypeptide. The method can include steps of (a) attaching a polypeptide to a particle or solid support, thereby producing an immobilized polypeptide having a plurality of amino acids linked to the particle or solid support; (b) fragmenting the immobilized polypeptide, whereby the particle is attached to a set of fragments of the polypeptide; (c) performing a binding assay including contacting the set of fragments with a plurality of affinity reagents and detecting binding of affinity reagents of the plurality of affinity reagents to the set of fragments; and (d) identifying the polypeptide from results of the binding assay.

Abstract: Methods of calibrating ELISA read-out for clinical antibody response are described. In particular, the invention relates to methods for calibrating ELISA read-out for the clinical antibody response to vaccines by quantitative mass spectrometry.

Abstract: Mycobacterial-specific biomarkers and methods of using such biomarkers for diagnosis of mycobacterial infection in a mammal are disclosed.

Abstract: The present disclosure is directed to human monoclonal IgE antibodies, and IgG antibodies engineered therefrom. Such engineered antibodies can be used to blunt pathologic IgE responses in subjects, such as in the treatment or prevention of allergies.

Abstract: The present disclosure relates generally to methods and chemical compositions for saliva sample processing and testing, and more specifically, rapid analysis of saliva biomarkers for detecting and monitoring disease.

Abstract: The present invention relates to compositions and methods for identifying at-risk patients and modulating thrombotic conditions in a cancer patient. Embodiments provided herein include a method of determining risk for a thrombotic event in a cancer patient comprising: detecting in a sample of a patient with cancer elevated levels of PPIA, PDIA3 and at least one of EIF5A, EIF4H, EIF4a3, UBE2N, UBE2L3, UBE2I, and HSP70.

Abstract: The disclosure relates generally to methods of treating allergies in subjects, methods of monitoring treatment of allergies in subjects, methods of diagnosing allergies in subjects, methods for determining whether subjects are candidates for allergy treatments, methods and kits for detecting allergies or allergens, methods for identifying cross-reactive allergens, antibodies that bind allergenic proteins and methods of identifying the same, and multiple allergen compositions and methods of preparing the same.

Abstract: The present disclosure provides methods and systems for diagnosing diseases and monitoring their progression and therapeutic responses by detecting a presence or absence, or an increase or decrease, of one or more substances in a sample. The methods may involve microflow liquid chromatography (LC) coupled with mass spectrometry (MS) to rapidly quantitate biomarkers in a sample and identify the likelihood of the sample being positive for a disease or condition.

Abstract: A unique pipeline is employed for biomarker discovery that entailed domain antibody phage display, next generation sequencing analysis, and nanotechnology strategies to generate antibody mimetics are disclosed. Also disclosed are the temporal biomarkers of traumatic brain injury and their methods of use. In some embodiments, the temporal biomarkers are synthetic peptides comprising the HCDR3 sequences identified using the disclosed pipeline. In some aspects, the synthetic peptides have less than 30 amino acid residues and comprise a biotin scaffold that is linked to the HCDR3 sequences.

Abstract: An object of the present invention is to provide a simple purifying method, measuring method, and kit. The present invention is an oxytocin purifying method including treating a sample with an acid or a salt thereof, and treating the sample treated with the acid or the salt thereof with a hydrophobic carrier, and related to the purifying method, measuring method including the purifying method, and kit, in which the acid or the salt thereof is at least one or more acids or salts thereof selected from the group consisting of hydrochloric acid, acetic acid, sulfuric acid, sodium hydrogensulfate, potassium hydrogensulfate, lithium hydrogensulfate, ammonium sulfate, and a mixture thereof, and the hydrophobic carrier is a hydrophobic carrier having at least one or more functional groups on the surface, selected from the group consisting of an alkyl group having 4 to 6 carbon atoms, an alkylene glycol group, and a phenyl group.

Abstract: An ultra-rapid and highly-sensitive chemiluminescence immunoassay for the detection of thyroid stimulating hormone uses water-soluble CdTe QDs as a chemiluminescence reagent and PBS solution of KMnO4 with specific concentration and pH as an activator to construct a CdTe QDs/KMnO4 chemiluminescence system of nanoparticle luminescence; based on the semi-automatic test method, the ultra-fast and highly sensitive detection of TSH can reach the level of single molecule detection.

Abstract: System, apparatus and method of testing a sample capable of transporting oxygen and including a dye, wherein the system includes: an illumination device to generate a first light pulse for a first period and second light pulses for each second period, the first pulse and second pulses illuminating the sample; a first detector to collect absorbance spectrum measurements from the sample illuminated by the first pulse; a second detector to collect phosphorescence measurements emitted by the dye of the sample illuminated by the second pulses, each phosphorescence measurement collected during a third period after a corresponding second pulse; a microcontroller to generate an absorbance spectrum average of the spectrum measurements, and to generate a phosphorescence average of phosphorescence measurements; and a computing device to calculate SatHb based on the spectrum average and calculate PO2 based on the phosphorescence average, and plot a point SatHb, PO2 on an oxygen equilibrium curve.

Abstract: Disclosed are an immunochemical assay device and a method of using the immunochemical assay device for detecting one or more targets or markers such as Cardiac Troponin I, NT-pro-BNP, D-dimer and/or cross-linked fibrin in a fluid sample.

Abstract: Cell deposition and staining apparatuses and methods are disclosed herein. In particular, the deposition and staining apparatuses disclosed herein provide low-volume, automated bench top systems for depositing and staining cellular samples on a cytological slide. An example deposition and staining apparatus includes a housing having an access door; a substrate processing holder located within the housing configured to hold one or more substrates and/or one or more substrate cartridges, wherein the substrate processing holder is accessible when the access door is in an open configuration; at least one opening located at least partially above at least a portion of the substrate processing holder; a spray nozzle configured to dispense a gaseous substance into the substrate processing area; a user interface configured receive an input from a user, and in response to receiving the input, cause execution of a pre-programmed protocol; and a waste and/or reagent holder element.

Abstract: Disclosed is a detachable modularized test platform. The detachable modularized test platform includes a gantry, a control box and a jig box, where a test area is arranged on the jig box, an air cylinder is arranged on the gantry, a control assembly is mounted in the control box, and a test assembly is mounted in the jig box; during testing, a sample to be tested is placed on the test area, and the upper computer sends an instruction to control the air cylinder to extend a driving rod to move to the sample to be tested; and the upper computer sends the instruction to the control assembly in the control box, to complete testing of the sample, and output and upload test data to a server for saving. The present invention employs a modularized structure, which reduces a size, detection is convenient, and applicability is high.

Abstract: A GUI is used to schedule a set of tasks for one or more robots to test samples using one or more instruments. A set of drivers are used to map instructions for the robots and instruments form a standardized format to a set of instrument/robot-specific formats. Using the standardized formats enables support for new types of robot and/or instrument to be easily added to the system. The robots and instruments generate test results from samples according to the instructions. Information about the testing may be stored in conjunction with user identifiers and other metadata to provide a verifiable audit trail across the instruments and robots.

Abstract: A method of determining a 3D center location of a specimen container on a track. The method includes providing a calibration object on the track; providing an initially calibrated image capture device adjacent to the track; moving the calibration object to at least two different longitudinal positions along the track; capturing a first image with the calibration object located at the first longitudinal position; capturing a second image with the calibration object located at the second longitudinal position; and determining a three-dimensional path trajectory of a center location along the track based at least upon the first image and the second image. The method can be used to determine a 3D center location of a specimen container imaged anywhere within a viewing area. Characterization apparatus and specimen testing apparatus adapted to carry out the methods are described, as are other aspects.

Abstract: A method of aligning a component to a structure in a diagnostic laboratory system. The method includes aligning a position sensor to the structure; sensing a position of the component using the position sensor; and calculating the position of the component relative to the structure based at least in part on the sensing. Other methods, apparatus, and systems are disclosed.

Abstract: An evaluation apparatus according to the present disclosure includes at least one memory storing a set of instructions, and at least one processor configured to execute the set of instructions and thereby to acquire, from a sensor, time-space data on vibrations that have occurred in an overhead optical fiber strung between poles, and evaluate, for each of predetermined analysis sections, a wind velocity and a propagation speed of wind in the analysis section based on the time-space data, and evaluate a wind direction in the analysis section based on the evaluated wind velocity and the propagation speed of the wind.