Abstract: A non-particulate organic porous material with optical resolution capability, the non-particulate organic porous material having a continuous pore structure, which comprises macropores and mesopores, the macropores being interconnected forming mesopores with a radius of 0.01-100 ?m in the interconnected parts, and optically active groups uniformly introduced therein possesses high physical stability, can be used under wide separating conditions, and has a large capacity for separating optically active substances (enantiometers).

Abstract: Disclosed is a separating agent for optical isomer which is excellent in optical separation ability. Specifically disclosed is a separating agent for optical isomer containing: an inclusion complex including a ?-conjugated polymer in a polymer compound having a hydroxy group or an amino group; and a carrier, the inclusion complex being carried by the carrier.

Abstract: To provide a medium suitable for high speed/high resolution, rich in hydrophilicity and resistant to a high concentration aqueous alkaline solution. A medium comprising crosslinked polymer particles containing from 20 to 95 mol % of repeating units derived from a (meth)acryloyl monomer represented by the formula (1): wherein R2 is a hydrogen atom or a C1-4 alkyl group, R1 is —NR3-R4-R5 or —O—R4-R5, R3 is a hydrogen atom or a C1-4 alkyl group, R4 is a C6-15 alkylene group containing an alicyclic ring or a C4-8 linear alkylene group, and R5 is a halogen atom, an alcoholic OH group, an amino group, a glycidyl group or an epoxy group.

Abstract: A sample separation apparatus (200) for separating a fluidic sample, the sample separation apparatus (200) comprising a first separation unit (204) for separating the fluidic sample, a first fluid drive (202) configured for conducting the fluidic sample to be separated through the first separation unit (204), a second separation unit (208), arranged downstream of the first separation unit (204), for further separating the fluidic sample after treatment by the first separation unit (204), a second fluid drive (206) configured for at least partially conducting the fluidic sample, after treatment by the first separation unit (204), through the second separation unit (208), and a fluidic valve (218) having fluidic interfaces (222, 224, 226, 228) fluidically coupled to the first fluid drive (202) and the second fluid drive (206) and being switchable for performing the separation of the fluidic sample, wherein the sample separation apparatus (200) is configured for adjusting a pressure at a predefined position to a

Abstract: An apparatus for chemical separations includes a first substantially rigid microfluidic substrate defining a first fluidic port; a second substantially rigid microfluidic substrate defining second fluidic port; and a coupler disposed between the first and second substrates, the coupler defining a fluidic path in fluidic alignment with the ports of the first and second substrates. The coupler includes a material that is deformable relative to a material of the first substrate and a material of the second substrate. The substrates are clamped together to compress the coupler between the substrates and form a fluid-tight seal.

Abstract: The present invention concerns new monolithic shaped bodies, a method for their preparation as well as their use especially for the selective purification and separation of biopolymers. A chromatographic separation material is provided with the new monolithic shaped bodies that allows a selective, efficient and reproducible purification and separation of biopolymers.

Abstract: In an embodiment, a porous composite particulate material includes a plurality of composite particles. Each composite particle includes an acid-base-resistant core particle at least partially surrounded by one or more layers of acid-base-resistant shell particles. The shell particles are adhered to the core particle by a polymeric layer. The shell particles and/or core particles may be made from an acid-base-resistant material that is stable in harsh chemical conditions. For example, the shell particles and/or core particles may be made from diamond, graphitic carbon, silicon carbide, boron nitride, tungsten carbide, combinations of the foregoing, or other acid-base-resistant materials. The porous composite particulate materials disclosed herein and related methods and devices may be used in separation technologies, including, but not limited to, chromatography, and solid phase extraction.

Abstract: Disclosed are methods for the chromatographic separation of rebaudioside A from stevioside in glycoside solutions that are derived from stevia. The chromatographic separation may be an adsorb/desorb type of chromatographic separation or a fractionation type of chromatographic separation.

Abstract: The present invention concerns a method of purifying colistin using reverse phase chromatography, wherein loading a column with colistin base in acetic acid and high ethanol concentration and eluting with low ethanol concentration is performed.

Abstract: The present invention is relates to method for production of a biomolecule adsorbent with a pentadentate ligand with very strong chelating properties, and to methods for use thereof for, for example, protein purification, detection or binding. The biomolecule adsorbent comprises the formula: wherein Q is a carrier, S is a spacer, L is an amide linkage, X is COOH, and n=2 or 3.

Abstract: A frit (44) includes a porous support structure having a plurality of void spaces, where a plurality of secondary particles (46) are filled in the void spaces, the secondary particles (46) being dimensioned with respect to the void spaces such that the frit (44) retains packing materials with diameters of less than about 2.5 microns. Preferably one or more frits (44) are received in a high pressure liquid chromatography (HPLC) chromatographic column, where the column includes fittings and filter assemblies for receiving frits (44) at its inlet and outlet. The frit (44) can be constructed from a porous support structure with void spaces or pores that are filled with secondary particles (46) smaller those used to manufacture the support. The particles (46) contained in the void spaces are large enough to be retained by the support, but small enough to create a finer network of interconnected channels within the support's void spaces that are capable of retaining sub-2.5 micrometer particles.

Abstract: The present invention provides methods for cleaning or regenerating a chromatography materiel for reuse. The methods of the invention can be used for cleaning or regenerating chromatography columns for reuse in the large-scale manufacture of multiple polypeptide products.

Abstract: Embodiments of the present invention feature methods and apparatus for performing chromatographic separations. The invention feature methods and control means in the form of computer programs and software that control the formation of a concentration gradient at two or more total flow rates, with an elution period occurring in a slow flow rate and at least other portions of the gradient occurring during a fast total flow rate.

Abstract: A chromatography column distribution system (101) comprises a set of first bed support ribs (107) extending radially from a inner, first radial position (R1) near the centre of the plate to a outer radial position nearer to the periphery (109) of the plate and at least one set of intermediate bed support ribs (117, 119) starting at an intermediate radial position (R2, R3) and extending to an outer radial position nearer to the periphery (109) of the plate (101), whereby channels are formed between adjacent bed support ribs (107, 117, 119).

Abstract: A device and method for making a microfluidic separation device. A microfluidic separation device could include a microfluidic column having an inlet, the microfluidic column being configured to hold a first fluid and the microfluidic column including a porous portion, and an outlet attached to the microfluidic column, the outlet being configured to output a second fluid. The method may include providing a microfluidic column having an inlet, configuring the microfluidic column to hold a first fluid, forming a porous portion in the microfluidic column, and attaching an outlet to the microfluidic column.

Abstract: Described are methods in which the retention times of a chromatographic column are adjusted through the control of the temperature of a mobile phase at the inlet to the chromatographic column. The temperature of the mobile phase at the inlet is different from the temperature of the chromatographic column. Adjustment of the temperature of the mobile phase at the inlet can be used as part of a method to transfer a chromatographic method from one liquid chromatography system to another liquid chromatography system. The method can alternatively be adapted for trapping a sample at the head of a chromatographic column to reduce the amount of band broadening of the sample from the sample injector of a liquid chromatography system. A gradient elution can then be performed to cause the concentrated volume of the analyte in the sample to elute in a tight band, resulting in improved measurement sensitivity.

Abstract: A multicapillary sample preparation device, especially useful for handling biological samples, comprising a plurality of uniform capillary tubes coated with a stationary phase, and arranged in a monolithic element. The multicapillary device is suitable for attachment to a pipette, micropipette, syringe, or other analytical or sample preparation instrument.

Abstract: The present invention relates to a holder for monolithic sorbents in which a radial pressure can be exerted over the entire length of the sorbent by a conically tapering clamping tube.

Abstract: A solution containing a target compound is passed through a trap column (14) to capture the target compound in the column (14). Thereafter, wash water is introduced in the trap column (14). Then, the setting of a six-port valve (7) is changed and an on/off valve (6) is opened to introduce nitrogen gas through a supply-side passage (8) in the trap column (14). In this stage, the nitrogen gas is warmed by means of a heat-exchange block (10), and the trap column (14) is warmed via a column rack (15). Any water remaining in the trap column (14) is initially thrust upwards by the nitrogen gas, to be extruded from an exit edge (14b) and discharged through a discharge-side passage (20). Furthermore, any water adhering to adsorbent or the inner wall surface of the trap column (14) is quickly vaporized and carried away by the nitrogen gas.

Abstract: LC/MS data generated by an LC/MS system is analyzed to determine groupings of ions associated with originating molecules. Ions are grouped initially according to retention time, for example, using retention time or chromatographic peaks in mass chromatograms. After initial groupings are determined based on retention time, ion peak shapes are compared to determine whether ions should be excluded. Ions having peak shapes not matching other ions, or alternatively a reference peak shape, are excluded from the group.

Abstract: Methods and related apparatuses and mixtures are described for chromatographic analysis. The described system includes a pressurized source of a mobile phase and a flow path in fluid communication with the pressurized source such that the mobile phase flows through the flow path. The system also includes an injector in fluid communication with the flow path and downstream of the pressurized source, the injector being configured to inject a sample into the flow path. A first column located downstream of the injector, contains a stationary phase, and forms part of the flow path. A first detector is positioned to detect properties of fluid in the flow path at a location downstream of the injector and upstream from the first column. A second detector is positioned to detect properties of fluid in the flow path at a location downstream of the first column.

Abstract: A mixed liquid passage (31) is connected to a trapping passage (22) at a point upstream of a trap column (23). A mixture of a diluting liquid and an additive agent, which are respectively drawn and sent by pumps (35) and (36), is supplied into the mixed liquid passage (31). When an eluate containing a target compound is passed through the trap column (23), the eluate is diluted with the diluting liquid and hence its elution capability decreases, so that the target compound can be easily captured in the trap column (23). Meanwhile, the solubility of the mobile phase in the eluate decreases, causing not only the target component but also foreign compounds to more easily precipitate. However, their precipitation is prevented by the effect of the additive agent. Thus, clogging of the trap column (23) or pipes is prevented.

Abstract: The present is a hydrophilic polymer microparticle, which has a DW/DA ratio of 2.0 or less, the DW representing a particle diameter of the hydrophilic polymer microparticle dispersed in water, the DA representing a particle diameter of the hydrophilic polymer microparticle dispersed in acetone, each of the particle diameters measured by a particle size distribution analyzer after the hydrophilic polymer microparticle is dispersed in water or acetone, subjected to irradiation with ultrasonic waves for 15 minutes, and allowed to stand at a temperature of 25° C. for 240 hours for equilibration; and has a contact angle with water of 70° or less, the contact angle measured using a contact angle measurement apparatus at a temperature of 25° C. by forming a droplet of pure water on a single layer of the hydrophilic polymer microparticles arranged with no space therebetween.

Abstract: Aimed at readily and exactly separate 8-hydroxy-2?-deoxyguanosine (8-OHdG) contained in a sample, a column packing material used for separating 8-OHdG, which contains a packing material composed of a material having a straight-chain hydrocarbon group having 6 or more and 30 or less carbon atoms as a functional group, and having a carbon content over the surface of carrier of 18% or less by element ratio, wherein the packing material contains 1 cumulative percent or more and 20 cumulative percent or less, on the particle-count basis, of particles having a circle-equivalent diameter, measured using a flow-type particle image analyzer, of 0.5 ?m or larger and 10 ?m or smaller, is used.

Abstract: Provided are methods for determining the amount of reverse T3 in a sample using mass spectrometry. The methods generally involve ionizing reverse T3 in a sample and detecting and quantifying the amount of the ion to determine the amount of reverse T3 in the sample.

Abstract: A method of liquid chromatography includes providing one or more solvent reservoirs, providing a solvent pump, drawing one or more solvents into the pump in response to a pressure drop that promotes outgassing of the solvents, and dispersing outgassed bubbles into smaller bubbles to promote re-dissolution of the gas. A liquid-chromatography apparatus includes at least two solvent reservoirs, a pump, at least one bubble-dispersing unit that receives a pressurized flow of proportioned solvents from the pump, and a control unit. The control unit includes a processor and a memory that stores instructions; the control unit controls proportioning of solvents to obtain a preselected solvent composition, and pumping at flow rates to support preparative-scale or process-scale liquid chromatography.

Abstract: This invention relates to methods for the evaluation and/or quantification of the binding affinity of small molecules or other compounds to target components contained within an analyte, such as target proteins contained within the proteome of a cell or tissue.

Abstract: The subject of the invention is a method for purifying the rabies virus, comprising a single ion-exchange chromatography step, said step being cation exchange chromatography according to which: a) the supernatant of a culture of cells infected with this virus is brought into contact with a cation exchange chromatography support comprising a polymethacrylate matrix onto which sulfoisobutyl groups have been grafted such that the rabies virus binds to this support, and; b) the virus is eluted from its support.

Abstract: Exemplary embodiments of the present disclosure relate to systems and methods comprising a dynamic pressure regulator and a force balance needle that regulate pressure changes due to flow or composition changes in a pressurized flow system, such as, for example, a CO2-based chromatography system.

Abstract: Disclosed is a chromatographic system and method which includes a passive pre-heater assembly. In the inventive system and method a fluid mixture is supplied from an injector to the pre-heater assembly and then to a chromatographic column having an active heating source. The pre-heater assembly includes a convoluted tube with first and second ends and a fluid passage extending centrally therebetween, first and second end fittings attached respectively to the first and second ends of the convoluted tube, and a thermally conductive film attached to an outer surface of the convoluted tube. The first end fitting is adapted for fluidly connecting the first end of the convoluted tube to the injector and the second end fitting is adapted for fluidly connecting the second end of the convoluted tube to the chromatographic column.

Abstract: A method and system for preconcentrating analytes at a microvalve in a microfluidic device is disclosed. The system includes a sample channel loaded with a sample solution. The sample channel includes a semi-permeable membrane microvalve. An electric potential is applied at or across the microvalve to preconcentrate the sample solution when the microvalve is closed.

Abstract: The present invention relates to a method of separating betaine and at least one other component from a sugar beet based fermentation solution. The invention is based on the use of a combination of SAC resins and WAC resins in a specific order and in specified proportions in a chromatographic SMB separation system. The chromatographic separation system is preferably a single integrated SMB system comprising both SAC and WAC resin beds.

Abstract: There is proposed a separation method for mixtures of materials, using a cellulose hydrate membrane having a porous double structure which consists of micropores having a diameter in the range from >100 nm to 20 ?m and ultrapores which have a diameter of <100 nm and which are not accessible to Blue Dextran having an average molecular weight Mw of 2 000 000, wherein the fraction of the volume of the ultrapores is more than 15% of the entire pore volume accessible to water, and wherein, in a preferred embodiment, sulfonic acid ligands are bonded to the membrane.

Abstract: A method is described to treat atherosclerosis by extracorporeally treating a patient's blood. A patient's blood is treated extracorporeally using a moiety that targets an antigen in the blood. The moiety facilitates removal of the blood antigen. The cleansed blood is then returned to the patient.

Abstract: The present invention discloses a silica particle having a diameter less than or equal to 2 ??, wherein the particle is spherical and comprises interconnected pores having a diameter in the range from 50 nm to 300 nm. The silica particle is preferably produced by spray pyrolysis (=spray drying) of a silica colloid. In the production process, porosity is introduced by means of an inorganic salt, such as NaCl, KCI, LiCl, NaNO3 or Ll NO3, which serves as a pore template. The silica particle may further be functionalized with proteins, peptides, nucleic acids, polysaccharides and proteoglycans, preferably concanavalin A or avidin. The present invention further discloses the use of the silica particle in chromatography, in particular in affinity chromatography.

Abstract: The invention provides a preparation method of super-macroporous polymeric microspheres and a product thereof. The method is characterized in that adding high content of surfactant into an oil phase containing a monomer and dispersing the oil phase containing the monomer and the surfactant into an aqueous phase. Super-macroporous microspheres are obtained by means of suspension polymerization. In the interior of the microspheres, 10-60% of the pores are distributed in a range of 500-900 nm. The microspheres have a particle diameter of 1-200 ?m and a porosity of 30%-90%. The product is suitable for being used as a stationary phase matrix in liquid chromatography, a carrier for high performance catalysts and a high performance adsorbent, and especially, being used as a separation medium for separating biomacromolecules.

Abstract: A column for use in chromatography, the column having a tube with a bore that can contain a packing medium captured between two porous plugs positioned at opposite ends. End fittings are attached to the tube ends, the end fittings having a duct with a duct opening having a diameter at least as large as the diameter of the tube bore which it faces. The duct opening may be positioned within a recess in spaced apart relation to the porous plugs to prevent the end fitting from contacting the plug and burnishing its surface.

Abstract: To use a monolithic silica body in chromatography with a HPLC column or a GC column and to simplify the use thereof as a separation medium, it is intended to provide a method of cladding a main body of a monolithic adsorbent or separating agent with glass so as to protect the outer surface, and to provide a separation medium prepared by the method. To this end, a monolithic silica body alone is formed by molding, and the molding is coated with a glass body; and then the glass body and the monolithic silica body are fused and integrated at the melting temperature of the glass body at an appropriate pressure. The surface of the resulting monolithic silica body clad with glass is strongly protected by the glass, and the homogeneity of the interior of the monolithic silica body is maintained, and thus uniform flow of a sample solution ensures analytical accuracy.

Abstract: Sieve valves for use in microfluidic device are provided. The valves are useful for impeding the flow of particles, such as chromatography beads or cells, in a microfluidic channel while allowing liquid solution to pass through the valve. The valves find particular use in making microfluidic chromatography modules.

Abstract: The present invention aims at providing a preparative separation/purification system for vaporizing an eluate in a short period of time, while enhancing the efficiency of collecting the target substance by accelerating the initiation of collecting the eluate.

Abstract: An apparatus for chemical separations includes a first substantially rigid microfluidic substrate defining a first fluidic port; a second substantially rigid microfluidic substrate defining a second fluidic port; and a coupler disposed between the first and second substrates, the coupler defining a fluidic path in fluidic alignment with the ports of the first and second substrates. The coupler includes a material that is deformable relative to a material of the first substrate and a material of the second substrate. The substrates are clamped together to compress the coupler between the substrates and form a fluid-tight seal.

Abstract: Disclosed is a process for the separation of one or more fractions from a resulting reaction mixture of an enzymatically catalyzed polymerization of flavonoid using HPLC with a varying concentration of organic solvent in the mobile phase. The isolated fractions can be used in numerous applications including as an antioxidant.

Abstract: The invention concerns a device comprising a base (2) and a door (20), said device having a closed door position in which a circuit (8) of the device comprises a bag comprising two flexible films and connectors of the conveying network, and a press (9) comprising a first she (16) disposed on a front face (5) of said base (2) and a second shed (17) disposed in said door (20); and a hinge system hinging said door (20) relative to said base (2), and disposed only on one side of said door (20) so as to form lateral clearances between said door (20) and said base (2) over the rest of a perimeter of said door (20)

Abstract: Methods and apparatus for desolvating flowing liquid streams while retaining temporal resolution of dissolved substrates are disclosed. A novel small-scale self-regulating spray dryer preserves temporal resolution while desolvating a liquid chromatography eluent stream and depositing the solute onto an optical surface for infrared spectrographic analysis. The liquid eluent is pumped through a heated nebulizer to create a high-speed jet of solute containing liquid and solvent vapor. This jet is directed circumferentially inside a hot cylindrical cavity. Centrifugal force causes the larger liquid droplets to travel along the outer diameter of the cavity. The cavity surface is heated to cause the droplets to film boil. Film boiling reduces droplet contact with the cavity surface thereby retaining the solute in the droplets. The solute temperature is limited by controlling the pressure into which the solvent evaporates from the droplets.

Abstract: Described is a disposable valve that includes an actuation layer operationally linked to a flexible diaphragm; a valving layer operationally linked to the flexible diaphragm; and a connection layer operationally connected to the valving layer; wherein the valving layer and the connection layer have a corresponding plurality of channels passing therethrough, and the channels define at least one fluid flow path, and the flexible diaphragm is movable between a first open position wherein fluid can flow through the fluid flow path, and a second closed position wherein fluid cannot flow through the fluid flow path. Chromatographic systems incorporating a plurality of such valves, optionally integrated into a unitary valve block, are also described.

Abstract: Disclosed is a chromatographic column for use with chromatographic materials packed in a defined manner. Said chromatographic column is characterized in that the chromatographic material in a defined compressed state is enclosed in a column body (1), which is preferably cylindrical and is provided with a discharge opening (9), between two filter plates which are fixed at a specified distance from one another by contact with support surfaces situated inside the column body.

Abstract: Separation methods based on electrophoresis or chromatography that use a stationary phase including fibril cellulose.

Abstract: Chromatography devices contain chromatography media and methods of making and methods of using chromatography devices. Chromatography devices enable a more efficient, productive and/or environmentally friendly chromatographic operation due to one or more of the following advantages over conventional chromatographic operations: elimination of a device packing step by the user; elimination of clean-in-place (CIP) steps; elimination of clean-in-place (CIP) steps utilizing sodium hydroxide solution; elimination of any validation steps by the user; and use of a chromatography device comprising biodegradable material. The chromatography media includes porous inorganic particles having a functionalized surface and having a median pore size of at least about 300 Angstroms (A), or at least about 300 A up to about 3000 A. The inorganic particles may have a BET surface area of at least about 20 m2/g, or at least about 25 m2/g, or about 30 m2/g, up to about 2000 m2/g.

Abstract: A sample dispatcher is disclosed and is configured for individually introducing a plurality of portions of one or more sample fluids into a flow of a mobile phase of a liquid separation system. The liquid separation system is configured for separating compounds of the sample fluids and comprises a mobile phase drive configured for driving the mobile phase through a separation unit configured for separating compounds of the sample fluids in the mobile phase. The sample dispatcher comprises one or more sample reservoirs, each configured for receiving and temporarily storing a respective sample fluid portion or at least a part thereof, and a bypass channel.

Abstract: Disclosed herein are methods and devices for dielectrokinetic chromatography. As disclosed, the devices comprise microchannels having at least one perturber which produces a non-uniformity in a field spanning the width of the microchannel. The interaction of the field non-uniformity with a perturber produces a secondary flow which competes with a primary flow. By decreasing the size of the perturber the secondary flow becomes significant for particles/analytes in the nanometer-size range. Depending on the nature of a particle/analyte present in the fluid and its interaction with the primary flow and the secondary flow, the analyte may be retained or redirected. The composition of the primary flow can be varied to affect the magnitude of primary and/or secondary flows on the particles/analytes and thereby separate and concentrate it from other particles/analytes.