Abstract: The present invention relates to a pipette (12) comprising a body portion (14) for aspirating a fluid sample (36) into a pipette tip (16) when attached thereto, wherein the body portion (14) comprises at least one light source (26), or an entry point for light from at least one light source, providing an optical path of light that passes through the sample (36) in a direction essentially along the longitudinal axis of the pipette tip (16). A method for measuring light output from a fluid sample (36), comprises providing a pipette tip (14) or capillary tube having first and second open ends (46, 68) with the sample (36) to be analysed contained therein, and detecting light output from an open end (46) of the pipette tip (16) or capillary tube. An apparatus and kit are also described. The pipette, apparatus, kit and method allow the accurate analysis and quantitative determination of minute amounts of biological samples.

Abstract: The invention concerns a sample chamber used for monitoring the concentrations of components of additives in a printing process liquid for maintaining predetermined desired concentrations of components of additives in a printing process liquid, wherein the actual concentrations of components are determined followed by redosing of measured components to a predetermined desired concentration.

Abstract: A system for and method of mapping process samples which are present in an environmental control chamber at a plurality of “X”-“Y” locations on the surface thereof, wherein the system includes a shield between windows for entering and exiting a beam of electromagnetic radiation, and a process sample.

Abstract: A blood analysis apparatus is provided. The blood analysis apparatus includes: a chip holding portion having an aperture which allows light to pass therethrough and holding a ?-TAS chip for holding a measurement liquid; a rotary body on which the chip holding portion is mounted; a light source; and a light-receiving unit. A measurement position of the rotary body at which the measurement liquid is to be measured with the light from the light source is set by: rotating the rotary body to obtain a light value of light which is emitted from the light source and received by the light-receiving unit through the aperture; and setting a rotational position of the rotary body where the light value is a threshold value or more, as the measurement position.

Abstract: Embodiments provide a handheld optical measuring device and method of measuring an optical property of a liquid sample. In some embodiments the optical measuring device includes a handheld controller module having an immersible sensor head and a sampling member including a sample cup and an attachment member that couples the sample cup to the handheld controller module. In some embodiments the attachment member is an elongated rigid member that is hingedly coupled to the controller module, thus providing a folding configuration for enclosing the sensor head with the sample cup during measurements, transportation, and/or storage. In some embodiments the attached sample cup provides a protective shell for the immersible sensor head during use and/or when not in use.

Abstract: An electrophoresis apparatus includes a multi-capillary array having a liquid or solid disposed between the capillaries of the array. The liquid or solid exhibits a refractive index higher than that of air and less than that of water and reduces the amount of laser beams scattered by the capillaries. Also provided are methods of adjusting refracted and reflected excitation light beams passing through capillaries of a multi-capillary array, to reduce loss of intensity of the laser beams and increase irradiation of respective samples disposed in the capillaries.

Abstract: A calibration card and method of using the card to calibrate an analytical instrument capable of reading a photoluminescent oxygen probe. The card includes at least (a) a first mass of an oxygen sensitive photoluminescent dye retained within a hermetically sealed space so as to isolate the dye from environmental oxygen, and in fluid communication with an activated metal-air battery whereby any oxygen permeating into the hermetically sealed space is quickly consumed by the battery, and (b) a second mass of an oxygen sensitive photoluminescent dye in fluid communication with the environment whereby the second mass of photoluminescent dye is exposed to an environmental concentration of oxygen.

Abstract: A pixel-by-pixel, digitally-addressable, pixelated, precursor, fluid-assay, active-matrix micro-structure including plural pixels formed preferably on a glass or plastic substrate, wherein each pixel, formed utilizing low-temperature TFT and Si technology, includes (a) at least one non-functionalized, digitally-addressable assay sensor, and (b), disposed operatively adjacent this sensor, digitally-addressable and energizable electromagnetic field-creating structure which is selectively energizable to create, in the vicinity of the at least one assay sensor, an ambient electromagnetic field environment which is structured to assist in functionalizing, as a possession on said at least one assay sensor, at least one digitally-addressable assay site which will display an affinity for a selected fluid-assay material.

Abstract: A digitally-addressable, pixelated, DNA fluid-assay, active-matrix micro-structure formed, utilizing low-temperature TFT and Si technology, on a substrate preferably made of glass or plastic, and including at least one pixel which is defined by (a) an addressable pixel site, (b) a sensor home structure disposed within that site for receiving and hosting a functionalized assay site possessing a DNA oligonucleotide probe, and (c) an addressable, pixel-site-specific, energy-field-producing functionalizer (preferably optical) operable to functionalize such a probe on the assay site. Each pixel may also include a pixel-integrated optical detector. Further disclosed are related methodology facets involving (1) the making of such a micro-structure (a) in a precursor form (without a functionalized probe), and thereafter (b) in a finalized/functionalized form (with such a probe), and (2) the ultimate use of a completed micro-structure in the performance of a DNA assay.

Abstract: A pixel-by-pixel digitally-addressable, pixelated, fluid-assay, active-matrix micro-structure including plural pixels formed preferably on a glass or plastic substrate, wherein each pixel, formed utilizing low-temperature TFT and Si technology, includes (a) at least one functionalized, digitally-addressable assay sensor including at least one functionalized, digitally-addressable assay site which has been affinity-functionalized to respond to a selected, specific fluid-assay material, and (b) disposed operatively adjacent that sensor and its associated assay site, digitally-addressable and energizable electromagnetic field-creating structure which is selectively energizable to create, in the vicinity of the sensor and its associated assay site, a selected, ambient, electromagnetic field environment which is structured to assist, selectively and optionally only, in the reading-out of an assay-result response from the assay sensor and assay site.

Abstract: Light emitting diodes (LEDs) are mounted in an array to an upper structure overlying a lower structure with a plurality of light detectors thereon. Each LED is configured to overlie a separate detector. Each LED emits light at a frequency relevant for measuring optical density of a specimen. LEDs having different frequencies are included within the LED array. A corresponding array of detectors is also provided, mounted to the lower structure. Spacing between adjacent LEDs and between adjacent detectors match a spacing between wells in a microtiter plate. Spacing between the lower structure and the upper structure supporting the LEDs is sufficient for the microtiter plate to fit between. Circuitry sequentially fires individual LEDs and gathers optical density data through the detectors for specimens in the wells of the microtiter plate. The structures are then moved to a next adjacent well position on the microtiter plate and the process repeated.

Abstract: An apparatus is described in which an optical fiber is mounted within a fiber optic holder which includes the non-rotating shaft of a linear actuator. The fiber holder may be held captive in order to restrain the fiber holder and, consequently, the fiber mounted therein, from rotating during operation of the linear actuator, thereby resulting in linear travel with minimal rotational effects and minimal change in optical alignment of the fiber during travel. In addition, an optical path length sensor in conjunction with an optimized absorbance method of operation is utilized herein to provide micron precision of the displacement between respective receiving and transmission fibers so as to enable precise absorbance measurements from about 0.005 up to about 2.0 Absorbance Units for any given path length.

Abstract: A microfabricated analysis chip with integrated waveguides for evanescent field absorption detection is provided. Fabrication of the microfluidic device may be performed by micropatterning a layer of photoresist or other suitable material using a single step photoresist process to produce a microchannel and an optical structure (e.g., a U-bend waveguide) in the microfluidic device. The microfluidic device couples a micro-channel network with a waveguide in a collinear fashion to maintain higher path length and incurring little or no scattering, dispersion, and divergence losses. A sample can be passed through the microchannel while light is transmitted through the waveguide. Any change in refractive index as well as change in optical absorbance property of fluid flowing in the microchannel can be detected by detecting changes in light output power (e.g., changes in absorbance). A change in concentration of solution in the microchannel can be determined by detecting a change in light absorbance output.

Abstract: An apparatus for counting and inspecting medicaments and other small objects whereby the objects are poured into a funnel. From the funnel, the objects fall onto the sharp point of a concentric cone, dispersing the objects on their way outwards causing dispersion and lateral singulation. Objects are vertically singulated when falling from the bottom edge of the cone. Objects are circularly scanned from just below the edge of the cone. A high speed processor resolves the scanned path in sufficiently small segments to determine width, and angular position measurements of the objects. The height measurements are resolved by the number of scans that show the objects in the same location before falling out of view. By calculations based on recurring sequential scans of objects at the same location, a total count can be made as well as sizes and irregularities of the objects.

Abstract: An integrated flow cell, the flow cell comprising a semiconductor substrate, and a fluidic conduit having an at least partially transparent semiconductor oxide tubing, wherein the semiconductor oxide tubing is formed with the semiconductor substrate.

Abstract: Apparatus for analysing a liquid sample (194) comprises beam generating means (1) for generating electromagnetic radiation, detector means (8) for detecting electromagnetic radiation from the beam generating means after the radiation has interacted with the sample, and sample retaining means (10) for releasably retaining sample in the path of the beam. The sample retaining means comprises a hydrophobic surface (for example, a coating on a plate 118) on which the sample is, in use, supported. There is also disclosed a method of performing photometric or spectrophotometric analysis of a liquid sample by sandwiching samples between two opposed hydrophobic support surfaces, passing a beam of electromagnetic radiation through one of the surfaces and then through the sample, and analysing the beam after it has passed through the sample.

Abstract: A dual-mode method and apparatus of selectively measuring samples in either a vessel or as a surface tension retained sample held between two opposing pedestals is introduced. In either configuration, such modes further contain optical paths from a source system through a small-volume or large-volume sample to a spectrometer based system. Such a system enables a user to measure samples with absorbances ranging from about 0.005 up to about 2.0 Absorbance Units for any given wavelength.

Abstract: This invention relates to optical cell geometry and to the arrangement of optical elements in an absorbance (at 200 nm to 50000 nm) detector. The invention allows to significantly expand the dynamic range of an optical density measuring instrument without affecting the signal to noise ratio. The invention also allows to enhance the precision of said instrument by eliminating the artifacts that arise from partial reflections of the incident beam, which occur on the passage of the beam through interfaces between different media.

Abstract: The present invention discloses a flow cell optical detection system comprising a light source, a flow cell and a light detector, wherein the light detector is arranged in a separate detector unit that is arranged to be releasably attached to a detector interface, the detector interface being in optical communication with the light source and comprises optical connectors for optically connecting the flow cell and the detector unit in the light path from the light source, and wherein the flow cell is an interchangeable unit arranged to be held in position by the detector unit when attached to the detector interface.

Abstract: This disclosure relates generally to a sampling device, and more particularly, a sampling device that facilitates spectroscopic measurements with a variable path length and the necessary software controlled algorithms and methods for such a device.

Abstract: Instrument-cartridge interfaces for fluidic analyzers that have an instrument and a removable cartridge are disclosed. For example, and in one illustrative embodiment, the instrument may include a needle that is adapted to penetrate a septum on a removable cartridge. In another illustrative embodiment, the instrument may include a plunger that is adapted to deform a deformable membrane on a removable cartridge. In yet another illustrative embodiment, the instrument may include a nozzle that is adapted to mate and seal with a flow channel on a removable cartridge. Techniques for detecting the flow rate in a flow channel on a removable cartridge, as well as the position of fluid in a flow channel of a removable cartridge, are also disclosed.

Abstract: A unit is provided comprising an array (2) of sample containers (1), said containers, being connected together and arranged in a planar configuration, each container having multiple optically transparent windows arranged such that the sample contained therein can be interrogated using simultaneous multiple optical analytical techniques, the array of containers being configured so as to allow optical access to the windows of each container in the array. Also provided is an apparatus comprising such a unit, a system comprising a combination of such an apparatus and unit and a method of analysing multiple samples by introducing each individual sample into an individual container of such an apparatus, illuminating the samples and detecting and analysing light emerging therefrom.

Abstract: The present invention is directed to the use of a light absorbing wall material to eliminate stray light paths in light-guiding structures, such as those used for HPLC absorbance detection. More specifically, the present invention relates to the use of carbon-doped Teflon® AF, or “black Teflon® AF,” for all or part of the walls of a light-guiding flowcell adapted for use in HPLC absorbance detection.

Abstract: Thermally controlled enclosures that can be used with gas analyzers are described. The enclosures incorporate one or more phase changing materials that buffer ambient and internal heat loads to reduce the power consumption demand of mechanical or electronic heating apparatus. Maintenance of gas analyzer equipment at a consistent temperature can be important to achieving stable and reproducible results. Related systems, apparatus, methods, and/or articles are also described.

Abstract: After a liquid sample between a sample-holding platform 11 and a window plate 22 is measured, a window plate holder 23 is raised, and a head 14 is moved from a standby position into the gap between the window plate 22 and the sample-holding platform 11. Then, the window plate holder 23 is lowered so as to press the window plate 22 onto the head 14 until the head 14 touches the sample-holding platform 11 below. Then the head 14 is swung back to the original position, whereby a wipe material 40 fitted on the head 14 simultaneously wipes off the liquid sample from both the lower surface of the window plate 22 and the upper surface of the sample-holding platform 11. Pressing the arch springs 35 enables the wipe material 40 to be easily attached to or detached from the head 14.

Abstract: A flow cell (200) for a sample separation apparatus (10) for separating components of a sample fluid in a mobile phase, the flow cell (200) being configured for detecting the separated components and comprising a tubing (202) having an inner wall (204) and an outer wall (206), the inner wall (204) defining a lumen (208) for conducting the sample fluid, the tubing (202) further having at one end an end face (210), and a detection unit (212) configured for detecting electromagnetic radiation (214) exiting the end face (210) after propagation of the electromagnetic radiation (214) through the sample fluid in a portion of the lumen (208) and through a portion of the tubing (202) between the inner wall (204) and the outer wall (206).

Abstract: The invention relates to a detector for measuring scattered light in liquids having a housing, a transparent, flexible tube for transporting liquid through the housing, a light emitter, and a light detector. Two parallel surfaces are formed in the housing, between which the tube is arranged such that two opposing tube walls are formed in a planar parallel manner. The light emitter is arranged in such a way that the optical axis thereof is perpendicular to the parallel surfaces of the first tube wall, and the light detector is adjacent to the light emitter, the optical axes of the light emitter and light detector forming an angle smaller than 90°. The invention also relates to a method for detecting the presence of blood and for the quantitative determination of biological marker substances, especially bilirubin, in solution, and to a device for treating blood containing the detector.

Abstract: A system includes a sensing cell having a walled structure configured to receive a fuel sample within an interior space of the walled structure. The sensing cell also has at least one cooling surface located on at least a portion of the walled structure and configured to cool the fuel sample. The sensing cell further has an optical port configured to couple to one or more optical fibers and to provide first radiation to the fuel sample. In addition, the sensing cell has a mirror configured to reflect the first radiation in order to provide second radiation to the optical port. The optical port defines a collinear optical geometry for providing the first radiation to the fuel sample and receiving the second radiation through the fuel sample. The system also includes at least one cooler configured to cool the fuel sample in the sensing cell by cooling the at least one cooling surface.

Abstract: A method and apparatus for determining a property of a medium, the apparatus including: a light source in a housing, for irradiating a space along a measuring path; a receiver in a housing, for registering intensity of light which has traversed the measuring path; wherein the measuring path enters through a first window section in a wall of the housing of the light source into the space, and wherein the measuring path leaves from the space through a second window section in a wall of the housing of the receiver. A medium to be measured is introducible in such a manner into the space, that the measuring path for a media measurement extends through the measured medium. At least one reference absorber, which is introducible at times into the space; and wherein the measuring path for at least one reference measurement extends through the reference absorber, when the reference absorber is brought into the space.

Abstract: Methods and apparatus for analyzing a sample using at least one detector are disclosed.

Abstract: A calibration tool for use in combination with a photoluminescent oxygen-sensitive working probe and an analytical instrument capable of reading the working probe. The calibration tool is effective for achieving two-point calibration of the analytical instrument, and includes at least first and second solid state compositions having different sensitivities to oxygen. The first composition is an oxygen-sensitive photoluminescent dye that is the same as that in the working probe, embedded within an oxygen-permeable carrier matrix that is the same as that in the working probe. The second composition is an oxygen-sensitive photoluminescent dye that is the same as that in the first composition, embedded within a carrier matrix that is different from that in the first composition. The oxygen sensitivity of the second composition is less than the oxygen sensitivity of the first composition.

Abstract: An optical measurement device is adapted to measure characteristics of a measurement targeted fluid based on a transmitted inspection light. The device comprises a cell having an internal space for accommodating the measurement targeted fluid to flow therein and having a pair of opposing through holes for transmitting the inspection light, each of the through holes sealed with a transparent member. The device includes a projection optical system member having a port for projecting the inspection light and a light-receiving optical system member having a port for receiving the inspection light transmitted through the internal space. A base member is provided for supporting the cell, the projection optical system member, and the light-receiving optical system member in a configuration with gaps between the cell and the respective optical system members to accommodate movement in a predetermined range along a direction perpendicular to an optical axis of the inspection light.

Abstract: A gas analyzer capable of measuring a concentration of a gas component in gas at sensor units provided at a plurality of positions in real time by decreasing the number of signals input from the sensor units to an analyzer so as to reduce a data amount input to the analyzer and a gas analyzing method. The gas analyzing method includes the steps of: demultiplexing laser light by a demultiplexer into measurement laser light and reference laser light; letting the measurement laser light pass through gas to be received by a photoreceiver; finding an absorption spectrum absorbed by a gas component in the gas based on a light intensity of the received measurement laser light and of the reference laser light; and analyzing the absorption spectrum to measure a concentration of the gas component.

Abstract: In a cavity mode of a cavity, a diameter of a mode waist is decreased so that the diameter is similar to a wavelength of an electromagnetic wave resonant with the cavity mode, when a material is located in the cavity. The material includes a physical system having two quantum states. A relative position between the material and the mode waist is scanned along three-directions unparallel mutually. A laser coupled with the cavity mode is input to the cavity. An intensity of at least one of a reflected light and a transmitted light of the laser from the cavity is measured.

Abstract: A device to measure the amount of light able to transmit through a test liquid sample. A single lamp is used to illuminate a liquid sample cell containing the test water. A light detector is fixed relative to the lamp and is used to detect the amount of light from the lamp able to transmit through the liquid sample cell. The liquid sample cell is shaped in such a way as to provide at least two sets of opposed side walls that are able to transmit the light emitted from the lamp, where each set of opposed side walls defines a different path length through the liquid sample in the liquid sample cell. A rotation mechanism is used to provide relative rotation between the liquid sample cell and the lamp/light detector assembly. A microprocessor connected to the light detector calculates the light transmitted through at least two different path lengths through the liquid sample. Using these calculated transmittances the microprocessor then calculates the overall transmittance of the test water.

Abstract: The invention relates to a detector for measuring scattered light in liquids having a housing, a transparent, flexible tube for transporting liquid through the housing, a light emitter, and a light detector. Two parallel surfaces are formed in the housing, between which the tube is arranged such that two opposing tube walls are formed in a planar parallel manner. The light emitter is arranged in such a way that the optical axis thereof is perpendicular to the parallel surfaces of the first tube wall, and the light detector is adjacent to the light emitter, the optical axes of the light emitter and light detector forming an angle smaller than 90°. The invention also relates to a method for detecting the presence of blood and for the quantitative determination of biological marker substances, especially bilirubin, in solution, and to a device for treating blood containing the detector.

Abstract: A liquid sample is dropped onto the upper surface of a transparent and cylindrical light-transmitting body (22), and the liquid sample is maintained as a droplet by the surface tension. From above the liquid sample, a transparent cover plate (25) is lowered down to the position where the lower surface thereof touches a spacer (23) in order that the liquid sample is held in the small gap formed between the upper surface of the light-transmitting body (22) and the lower surface of the transparent cover plate (25). A measurement light is provided into the liquid sample held in this manner from immediately above it, and passes through the liquid sample. The transmitted light emitted downwards through the light-transmitting body (22) is introduced into a spectro-detecting unit to be spectro-measured. The measurement optical path length can be adjusted by the height of the spacer (23). This enables an easy transmission spectro-measurement of an extremely small amount of liquid sample.

Abstract: An electrophoresis apparatus includes a multi-capillary array having a liquid or solid disposed between the capillaries of the array. The liquid or solid exhibits a refractive index higher than that of air and less than that of water and reduces the amount of laser beams scattered by the capillaries. Also provided are methods of adjusting refracted and reflected excitation light beams passing through capillaries of a multi-capillary array, to reduce loss of intensity of the laser beams and increase irradiation of respective samples disposed in the capillaries.

Abstract: A method and device for measuring trace levels of particles in an air sample is described. A device operating in a gradiometer configuration with two cavities built from a monolithic structure and utilizing a single probe laser, provides common mode subtraction of acoustic, vibrational, laser intensity and other noise sources, which allows sensitivity more closely approaching the quantum limit. Differential measurements between the two cavities occur simultaneously, which reduces errors due to cavity drift. Absorptive gradiometry can therefore provide noise immune detection for trace gasses, including broad linewidth absorbers where frequency-noise immune schemes are not practical. Differential measurements can be used for background subtraction, sensing vapor plume gradients and determining vapor plume propagation direction.

Abstract: An apparatus and method for estimating a parameter of interest in a downhole fluid using fluid testing module. The fluid testing module may include: a substrate comprising at least one microconduit, and a sensor. The sensor may be disposed within the at least one microconduit or external. The apparatus may include a fluid transporter for moving fluid within the microconduit. The method includes estimating a parameter of interest using the fluid testing module.

Abstract: Embodiments provide a handheld optical measuring device and method of measuring an optical property of a liquid sample. In some embodiments the optical measuring device includes a handheld controller module having an immersible sensor head and a sampling member including a sample cup and an attachment member that couples the sample cup to the handheld controller module. In some embodiments the attachment member is an elongated rigid member that is hingedly coupled to the controller module, thus providing a folding configuration for enclosing the sensor head with the sample cup during measurements, transportation, and/or storage. In some embodiments the attached sample cup provides a protective shell for the immersible sensor head during use and/or when not in use.

Abstract: Characteristics of a chemical or biological sample are detected using an approach involving light detection. According to an example embodiment of the present invention, an assaying arrangement including a light detector is adapted to detect light from a sample, such as a biological material. A signal corresponding to the detected light is used to characterize the sample, for example, by detecting a light-related property thereof. In one implementation, the assaying arrangement includes integrated circuitry having a light detector and a programmable processor, with the light detector generating a signal corresponding to the light and sending the signal to the processor. The processor provides an output corresponding to the signal and indicative of a characteristic of the sample.

Abstract: This disclosure relates generally to a sampling device, and more particularly, a sampling device that facilitates spectroscopic measurements with a variable path length and the necessary software controlled algorithms and methods for such a device.

Abstract: A closed cell for use in spectrophotometers is adapted to receive a liquid to be subjected to analysis and an air space or bubble to absorb pressure differentials, wherein the cell includes means to retain the air space or bubble in a predetermined location in the cell.

Abstract: An optical waveguide is provided comprising a non-solid core layer surrounded by a solid-state material, wherein light can be transmitted with low loss through the non-solid core layer. A vapor reservoir is in communication with the optical waveguide. One implementation of the invention employs a monolithically integrated vapor cell, e.g., an alkali vapor cell, using anti-resonant reflecting optical waveguides, or ARROW waveguides, on a substrate.

Abstract: Assay cartridges are described that have a detection chamber, preferably having integrated electrodes, and other fluidic components which may include sample chambers, waste chambers, conduits, vents, bubble traps, reagent chambers, dry reagent pill zones and the like. In certain embodiments, these cartridges are adapted to receive and analyze a sample collected on an applicator stick. Also described are kits including such cartridges and a cartridge reader configured to analyze an assay conducted using an assay cartridge.

Abstract: An optical measurement apparatus can be provided, in which the sample is optically measured without loss of the illuminating light with high sensitivity. A glass plate as the transparent member 50 is placed in the interface between the end face 43 of the optical waveguide 40 guiding the illuminating light L generated by the laser light source 20 and the wall face of the capillary 30. According to the above feature, the air layer is prevented from existing in the interface between the end face 43 of the optical fiber 40 and the wall face of the capillary 30, thus the sample S can be optically measured with high sensitivity and few variability without causing the loss of the illuminating light L.

Abstract: A mixing bag for use in bioprocessing receives a fluid and includes a sensing element for measuring at least one property thereof. The bag may include an internal fluid-agitating element and an integral sparger. Related methods are also disclosed.

Abstract: High resolution particle differentiation process and separation system that provides enhanced resolution of particles based upon selected particle characteristics. In particular, the system may include an enhanced resolution flow cytometer. In an embodiment, the invention can include at least one fluid source conduit (24) that introduces 0 fluid source stream (24) into an enhanced resolution nozzle (25) at an angle that enhances particle resolution by the cell sensing system (13).

Abstract: A system and method for creating a unique optical signature that uses a plurality of optical signature chambers containing fluids having differing optical properties, for example differing indices of refraction, that produce different individual optical signatures for each chamber. If the chambers are broken causing the fluids to spill or the fluids are otherwise discharged from the chambers, the fluids mix together so one does not know which fluid came from which chamber, thereby preventing replication of the correct optical signatures. This destruction of the optical signatures can occur mechanically, without the application or presence of electrical power.