Automated System With Sample Fluid Pressure Transport Means Patents (Class 422/81)
  • Patent number: 10352891
    Abstract: The application relates to an electrode for use in the electrochemical detection of target species, and in particular for the detection of insulin. The application enables insulin to be detected with high selectivity and with a detection limit in the picomolar range or lower. The electrodes of the invention can easily be reused and are ideally suited for use in point-of-care diagnostics. In a preferred embodiment, the electrode comprises an antibody modified, polyethylene glycol (PEG) monolayer assembled on a gold surface. In a second embodiment, the electrode comprises a chemisorbed zwitterionic carboxybetaine polymer obtained by photopolymerisation of carboxybetaine methacrylate (CBMA). This allows the use of non-Faradaic analysis to determine the insulin concentration even in undiluted blood serum.
    Type: Grant
    Filed: July 2, 2013
    Date of Patent: July 16, 2019
    Assignee: Oxford University Innovation Limited
    Inventor: Jason Davis
  • Patent number: 10131867
    Abstract: The present invention relates to a device for putting material into a cell to be modified. More particularly, the present invention is directed to a device formed within one solid material and comprises, a first passage on which the cell passes; a second passage on which the material passes and connected to the first passage at a position randomly selected between both ends of the first passage; and an apparatus which applies pressure difference or electric potential difference on the first passage and the second passage, and is also directed to a process for making the device.
    Type: Grant
    Filed: December 28, 2015
    Date of Patent: November 20, 2018
    Assignee: FEMTOBIOMED INC.
    Inventor: Sanghyun Lee
  • Patent number: 9846114
    Abstract: Technology is provided for a fluid transmissometer manifold. The transmissometer manifold includes a manifold body having an upwardly extending bubble diverter passageway with an upper end portion and a lower end portion. A flow restrictor is connected to the upper end portion and an inlet passageway is connected to the diverter passageway between the flow restrictor and the lower end portion. An upwardly extending optical chamber is connected to the lower end portion. At least a portion of a fluid entering the inlet passageway flows downward into the optical chamber and any bubbles contained in the fluid travel upward through the bubble diverter passageway. A light source can be positioned at a first end of the optical chamber and a detector positioned at a second end of the optical chamber opposite the light source and operative to detect light emitted from the light source.
    Type: Grant
    Filed: March 9, 2016
    Date of Patent: December 19, 2017
    Assignee: WET Labs, Inc.
    Inventors: Michael Dewey, Corey Koch
  • Patent number: 9778278
    Abstract: A sample-taking unit having a sampling unit and a hold-down device which is movable relative thereto in a parallel manner, said sample-taking unit including a self-holding mechanism which, as a result of a self-locking effect, automatically fixes the hold-down device in a lowered fixing position against an upward movement until the self-locking effect is removed again as a result of the self-holding mechanism being acted upon with a detaching force, preferably as a result of contact between the sampling unit and a clamping element of the self-holding mechanism.
    Type: Grant
    Filed: July 6, 2015
    Date of Patent: October 3, 2017
    Assignee: DIONEX SOFTRON GMBH
    Inventors: Hermann Hochgraeber, Robert Springer
  • Patent number: 9770716
    Abstract: The invention is directed to multi-fluidic cartridges for sample analysis and to methods of using said cartridges. In one embodiment, the cartridge comprises: (a) a first conduit beginning at a sample entry port for receiving a fluid sample and in fluid communication with one or more sensors; (b) a plurality of rupturable fluidic pouches, each containing a different fluid and in fluid communication with a respective delivery conduit configured for delivering a respective fluid to said first conduit; and (c) at least one pneumatic pump configured to move said fluid sample to said one or more sensors and for transporting at least one of said different fluids to said first conduit.
    Type: Grant
    Filed: August 29, 2014
    Date of Patent: September 26, 2017
    Assignee: Abbott Point of Care Inc.
    Inventor: Cary James Miller
  • Patent number: 9689866
    Abstract: A device for remote management of patients suffering from heart failure and hypertension can measure one or more biomarker. The device aids in monitoring the efficacy and safety of treatment in such patients.
    Type: Grant
    Filed: January 29, 2013
    Date of Patent: June 27, 2017
    Assignee: INVERNESS MEDICAL SWITZERLAND GMBH
    Inventors: Paul Sheard, Marika Reay
  • Patent number: 9677989
    Abstract: The present invention, provides a flow cytometry apparatus for the detection of particles from a plurality of samples comprising: means for moving a plurality of samples comprising particles from a plurality of respective source wells into a fluid flow stream; means for introducing a separation gas between each of the plurality of samples in the fluid flow stream; and means for selectively analyzing each of the plurality of samples for the particles. The present invention also provides a flow cytometry method employing such an apparatus.
    Type: Grant
    Filed: January 26, 2016
    Date of Patent: June 13, 2017
    Assignee: STC.UNM
    Inventors: Larry A. Sklar, Bruce Edwards, Frederick Kuckuck
  • Patent number: 9625367
    Abstract: In flow cytometry, particles (2) can be distinguished between populations (8) by combining n-dimensional parameter data, which may be derived from signal data from a particle, to mathematically achieve numerical results representative of an alteration (48). An alteration may include a rotational alteration, a scaled alteration, or perhaps even a translational alteration. Alterations may enhance separation of data points which may provide real-time classification (49) of signal data corresponding to individual particles into one of at least two populations.
    Type: Grant
    Filed: August 5, 2015
    Date of Patent: April 18, 2017
    Assignee: Beckman Coulter, Inc.
    Inventors: George C. Malachowski, Paul Barclay Purcell, Edward Allan Stanton, Kenneth Michael Evans
  • Patent number: 9588132
    Abstract: Method for validating the accuracy of automated analyzers by performing an improved dual dye ratio method procedure that uses at least first and second dye solutions in combination with gravimetric measurement of selected test solutions.
    Type: Grant
    Filed: January 4, 2015
    Date of Patent: March 7, 2017
    Assignees: Artel, Inc., Uniflex Company, Ltd.
    Inventors: Akira Hattori, George Rodrigues, Axel Bjoern Carle
  • Patent number: 9494613
    Abstract: A modular flow injection analysis system has a sample connection, a reagent inflow and a media discharge. The analysis system is formed of a plurality of functional modules stacked one on top of the other. These functional modules include a metering module including a metering channel having an inlet and an outlet, a mixing module including a mixing channel having an inlet and an outlet, an analysis module including a media chamber connected to the outlet of the mixing channel, and a media control module. The modules are connected by aligned media channels. These media channels replace the conventional tube connections. They are connected automatically when the modules are fitted together.
    Type: Grant
    Filed: May 18, 2010
    Date of Patent: November 15, 2016
    Assignee: BUERKERT WERKE GMBH
    Inventors: Christian Oberndorfer, Michael Winkler, Marco Zuern
  • Patent number: 9453789
    Abstract: Provided herein are improved cell sorter systems and methods. Such systems and methods provide a self-stabilizing sorter jet to automate calibration, and address the issue of drift in cell sorting systems. The systems and methods presented make it possible to determine and set the charge delay interval automatically with circuitry in the cell sorter. These circuits can set, monitor, and adjust the time delay continuously, allowing for a completely automatic, autonomous, turn-key, self-stabilizing sorter jet.
    Type: Grant
    Filed: October 26, 2015
    Date of Patent: September 27, 2016
    Assignee: Becton, Dickinson and Company
    Inventor: Ger van den Engh
  • Patent number: 9395361
    Abstract: The invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.
    Type: Grant
    Filed: June 5, 2015
    Date of Patent: July 19, 2016
    Assignees: ADVANCED LIQUID LOGIC, INC., DUKE UNIVERSITY
    Inventors: Vamsee K. Pamula, Michael G. Pollack, Vijay Srinivasan, Richard B. Fair
  • Patent number: 9372133
    Abstract: According to the microchemical chip of the present invention, the sample introducing port 11 is formed as a hole penetrating a face and a back of the first substrate 10, the sample flow path 21 is formed as a slit penetrating a face and a back of the second substrate 20, the sample discharging port 31 is formed as a hole penetrating a face and a back of the third substrate 30, the second substrate 20 is disposed between the first substrate 10 and the third substrate 30, the sample introducing port 11 and the sample discharging port 31 are in communication with each other through the sample flow path 21, and one of ends of the sample flow path 21 is an opening port, and thus, a square hollow groove including an angle having one piece of about 100 microns can be produced as the sample flow path 21.
    Type: Grant
    Filed: June 29, 2011
    Date of Patent: June 21, 2016
    Assignee: METABOSCREEN CO., LTD.
    Inventors: Ryuichi Sekizawa, Ryoko Aso
  • Patent number: 9170187
    Abstract: A flow cytometer and a fluid system are provided. The fluid system comprises a flow cell, a sample providing unit, a waste container, a sheath container, a negative pressure source, a quantitative unit and a sample flow monitoring unit, the negative pressure source, the waste container and the flow cell are connected, a negative pressure source, which provides a negative pressure relative to the sample providing unit for the flow cell so that the sample providing unit causes the sample to flow into the flow cell under the negative pressure, a sample flow monitoring unit monitors a flow of the sample fluid transported from the sample providing unit to the flow cell and outputs a feedback signal reflecting flow changes of the sample fluid in real-time; wherein the controller receives the feedback signal and controls the quantitative unit to adjust a flow of the sheath fluid according to the feedback signal.
    Type: Grant
    Filed: February 10, 2015
    Date of Patent: October 27, 2015
    Assignees: SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS CO., LTD., BEIJING SHEN MINDRAY MEDICAL ELECTRONICS TECHNOLOGY RESEARCH INSTITUTE CO., LTD.
    Inventors: Baohua Yan, Chuixin Liao, William Li, Huawen Yan, Wenheng Guo
  • Patent number: 9134204
    Abstract: A method and device for generating an ongoing consistent velocity within a pipeline flow in order to achieve fluid homogeneity prior to oil water measurement. A recirculation flow loop withdraws flow from a first point in a pipeline, accelerates that flow with a pump, and reinjects the outflow from the pump through turbulence inducing outlets at a second point in the pipeline upstream of the first flow withdrawal point. The increased turbulence and mixing of the fluid flowing in the pipeline enhances the homogeneity of the oil/water suspension. The more homogeneous fluid recirculating in the flow loop passes through a measurement probe of an oil/water detector, thus increasing the accuracy of the oil/water measurement of the fluid.
    Type: Grant
    Filed: July 17, 2012
    Date of Patent: September 15, 2015
    Assignee: KAM Controls, Inc.
    Inventor: Kam Mohajer
  • Patent number: 9110010
    Abstract: A device having: a laminar flow channel for liquids; two or more electrodes; a confining fluid inlet; a sample inlet; and a meter for measuring the impedance of any fluid between the electrodes. The device may have one or more specific binding sites, or it may have sheathing and unsheathing fluid transporting structures. A method of: providing the device; flowing a confining fluid and a conductive liquid that may contain cells or particles through the channel as described herein; and measuring the impedance between the electrodes.
    Type: Grant
    Filed: May 9, 2008
    Date of Patent: August 18, 2015
    Assignee: The United States of America, as represented by the Secretary of the Navy
    Inventors: Daniel A. Ateya, Peter B Howell, Jr., Frances S. Ligler
  • Patent number: 9081007
    Abstract: The present invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.
    Type: Grant
    Filed: August 22, 2014
    Date of Patent: July 14, 2015
    Assignee: ADVANCED LIQUID LOGIC, INC.
    Inventors: Michael G. Pollack, Vamsee K. Pamula, Ramakrishna Sista, Arjun Sudarsan
  • Patent number: 9039973
    Abstract: The present invention provides a hybrid digital and channel microfluidic device in the form of an integrated structure in which a droplet may be transported by a digital microfluidic array and transferred to a microfluidic channel. In one aspect of the invention, a hybrid device comprises a first substrate having a digital microfluidic array capable of transporting a droplet to a transfer location, and a second substrate having a microfluidic channel. The first and second substrates are affixed to form a hybrid device in which an opening in the microfluidic channel is positioned adjacent to the transfer location, so that a droplet transported to the transfer location contacts the channel opening and may enter the channel. The invention also provides methods of performing separations using a hybrid digital and channel microfluidic device and methods of assembling a hybrid digital microfluidic device.
    Type: Grant
    Filed: October 13, 2009
    Date of Patent: May 26, 2015
    Assignee: THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO
    Inventors: Michael W. L. Watson, Mohamed Abdelgawad, Mais Jebrail, Hao Yang, Aaron R. Wheeler
  • Patent number: 9005545
    Abstract: The present invention relates to a method for detecting the presence and/or the reaction of a biomolecule by monitoring changes of electrical property accurately according to the biological, biochemical or chemical reaction of the biomolecule, and a biochip provided for this purpose.
    Type: Grant
    Filed: December 7, 2012
    Date of Patent: April 14, 2015
    Assignee: Digital Genomics Inc.
    Inventor: Jae-Hoon Lee
  • Patent number: 8986449
    Abstract: The invention relates to a microfluidic device for promoting crystallization of target molecules, such as proteins. The device comprises a solid structure with a top face and an opposite bottom face and with a least one liquid channel. The liquid channel comprises a target molecule solution inlet and at least two precipitant inlets. The target molecule solution inlet is in liquid communication with each of the precipitant inlets through the liquid channel. The liquid channel comprises a branching channel section adjacent to the target molecule solution inlet, crystallization channel sections adjacent to the respective precipitant inlets and flow break channel sections arranged between the branching channel section and each of the crystallization channel sections.
    Type: Grant
    Filed: June 27, 2007
    Date of Patent: March 24, 2015
    Assignee: Microlytic North America Inc.
    Inventor: Morten Sommer
  • Patent number: 8989859
    Abstract: Methods and apparatus are provided, for selective destruction or temporary disruption of nerves and/or conduction pathways in a mammalian body for the treatment of pain and other disorders. Apparatus comprises catheters having electrodes for targeting and affecting nerve tissue at a cellular level to reversible and irreversible nerve portion and incapacitation.
    Type: Grant
    Filed: January 23, 2014
    Date of Patent: March 24, 2015
    Assignee: Medtronic Ardian Luxembourg S.a.r.l.
    Inventors: Mark E. Deem, Hanson Gifford, III
  • Patent number: 8968661
    Abstract: A sample analyzer prepares a measurement sample from a blood sample or a body fluid sample which differs from the blood sample; measures the prepared measurement sample; obtains characteristic information representing characteristics of the components in the measurement sample; sets either a blood measurement mode for measuring the blood sample, or a body fluid measurement mode for measuring the body fluid sample as an operating mode; and measures the measurement sample prepared from the blood sample by executing operations in the blood measurement mode when the blood measurement mode has been set, and measuring the measurement sample prepared from the body fluid sample by executing operations in the body fluid measurement mode that differs from the operations in the blood measurement mode when the body fluid measurement mode has been set, is disclosed. A computer program product is also disclosed.
    Type: Grant
    Filed: May 10, 2013
    Date of Patent: March 3, 2015
    Assignee: Sysmex Corporation
    Inventors: Takaaki Nagai, Noriyuki Narisada, Daigo Fukuma
  • Patent number: 8968660
    Abstract: A chemical analyzer analyzes a sample for a target chemical. The sample and reagent are added to a measurement cell until a detectable change is observed. A reference compound, which does not take part in the reaction between the target chemical and reagent, is added to the reagent. By measuring the concentration of the reference compound, the amount of reagent used may be determined. In one embodiment, a mechanism introduces the sample, adds reagent, mixes, cleans, and flushes the measurement cell, which incorporates a longitudinal chamber having inlets for the sample and reagent and an outlet for measured sample/reagent mixtures. A detector located in or adjacent to the chamber between the inlets and outlet and a piston movable in the chamber carries seals such that movement of a piston sequentially opens the inlets and expels the fluid. The analyzer also includes a device that delivers reagent to a measurement cell.
    Type: Grant
    Filed: August 17, 2011
    Date of Patent: March 3, 2015
    Assignee: Aqualysis Pty Ltd
    Inventors: Stephen Charles Davis, John David Petty, John Thomas Huberts
  • Patent number: 8962258
    Abstract: Provided are a multiple immunoassay apparatus on a chip in which a structure comprising multiple microfluidic channels is associated with a tissue sample, which allows immunohistochemical reactions to be conducted therein, to examine various markers specific for certain diseases, and a method for performing multiple immunoassays using the same. The multiple immunoassay apparatus comprises: at least one antibody-introducing unit through which at least one antibody is introduced into the apparatus; at least one reaction unit in which the antibody reacts with a sample in an immunohistochemical pattern; and at least one fluid outlet through which a fluid including the antibody is discharged outside the apparatus.
    Type: Grant
    Filed: August 29, 2008
    Date of Patent: February 24, 2015
    Assignees: Korea Advanced Institute of Science and Technology, National Cancer Center
    Inventors: Je-Kyun Park, MinSeok Kim, Eun Sook Lee, Sun-Young Kong, Solm Kwon
  • Publication number: 20150050719
    Abstract: A dispensing assembly comprising a cartridge holder is presented. The cartridge holder can receive a cartridge for dispensing a fluid. The cartridge can comprise a reservoir for receiving the fluid. The reservoir can comprise an outlet. The reservoir can have an adjustable volume for forcing the fluid through the outlet. The cartridge can further comprise a nozzle for dispensing the fluid. The nozzle can be connected to the outlet. The dispensing assembly can further comprise an actuator for actuating the adjustable volume. The dispensing assembly can further comprise an impulse generator for imparting an impulse to the nozzle. The impulse generator can comprise an actor for contacting the nozzle. The dispenser assembly can further comprise a controller for controlling the actor and the impulse generator.
    Type: Application
    Filed: November 3, 2014
    Publication date: February 19, 2015
    Inventors: Stefan Borja Bammesberger, Andreas Ernst, Peter Koltay, Nadine Losleben, Laurent Tanguy
  • Patent number: 8951805
    Abstract: A system for managing bulk liquids for an automated clinical analyzer. The system comprises (a) at least one local reservoir for storing a bulk liquid for impending use, (b) at least one container for holding a bulk liquid before the liquid is transferred to a local reservoir, and (c) a controller for monitoring the level of a bulk liquid in a local reservoir. The local reservoir for storing a bulk liquid for impending use can be a trough. The use of troughs for storing a reagent, a diluent, or some other treating agent for impending use enables an aspirating/dispensing device having a plurality of pipettes to aspirate and dispense the reagent, diluent, or other treating agent at a high rate of throughput. The controller can monitor the level of a liquid in (a) a local reservoir for storing a bulk liquid for imminent use and the level of liquid in a (b) container for holding a bulk liquid before the liquid is transferred to a local reservoir.
    Type: Grant
    Filed: April 8, 2014
    Date of Patent: February 10, 2015
    Assignee: Abbott Laboratories
    Inventors: Patrick P. Fritchie, Gregory E. Gardner, Richard W. Mahoney
  • Patent number: 8936755
    Abstract: Disclosed is an apparatus for analyzing the composition of bodily fluid. The apparatus can include a fluid handling network including a patient end configured to maintain fluid communication with a bodily fluid in a patient and a pump unit in operative engagement with the fluid handling network. The pump unit can have an infusion mode, in which the pump unit is operable to deliver infusion fluid to the patient through the patient end, and a sample draw mode, in which the pump unit is operable to draw a sample of the bodily fluid from the patient through the patient end. The apparatus can include a spectroscopic analyzer positioned to analyze at least a portion of the sample; a processor in communication with or incorporated into the spectroscopic analyzer; and stored program instructions executable by the processor to obtain measurements of two or more properties of the sample.
    Type: Grant
    Filed: March 16, 2012
    Date of Patent: January 20, 2015
    Assignee: OptiScan Biomedical Corporation
    Inventors: Jennifer H. Gable, James R. Braig, Kenneth I. Li, Mark Wechsler, Peng Zheng, Richard Keenan
  • Patent number: 8926902
    Abstract: An automatic apparatus is described, which is suitable for the withdrawal of portions of biological material from a parent test tube (1), mobile in a conveyor (6) of a test tube transport plant by means of transport devices (8) of single test tubes, to be loaded in one or more child test tubes (2) countermarked beforehand with suitable identification means and to be sent to different biological material analysis instruments interfaced to said transport plant.
    Type: Grant
    Filed: March 12, 2010
    Date of Patent: January 6, 2015
    Assignee: Inpeco Holding Ltd.
    Inventor: Gianandrea Pedrazzini
  • Publication number: 20140370583
    Abstract: The invention is directed to multi-fluidic cartridges for sample analysis and to methods of using said cartridges. In one embodiment, the cartridge comprises: (a) a first conduit beginning at a sample entry port for receiving a fluid sample and in fluid communication with one or more sensors; (b) a plurality of rupturable fluidic pouches, each containing a different fluid and in fluid communication with a respective delivery conduit configured for delivering a respective fluid to said first conduit; and (c) at least one pneumatic pump configured to move said fluid sample to said one or more sensors and for transporting at least one of said different fluids to said first conduit.
    Type: Application
    Filed: August 29, 2014
    Publication date: December 18, 2014
    Inventor: Cary James Miller
  • Patent number: 8894930
    Abstract: A specimen processing device is disclosed that comprises: a processing unit configured to aspirate a specimen from a specimen container accommodating the specimen, and to process the aspirated specimen; a state transition section configured to make the processing unit undergo transition to a pause state; an instruction accepting section configured to accept an instruction to start processing of the specimen when the processing unit is in the pause state; and a pause state releasing section configured to release the processing unit from the pause state to make the processing unit perform the processing of specimen when the instruction to start the processing is accepted by the instruction accepting section. A specimen processing method using a specimen processing device is also disclosed.
    Type: Grant
    Filed: May 26, 2010
    Date of Patent: November 25, 2014
    Assignee: Sysmex Corporation
    Inventor: Toru Mizumoto
  • Patent number: 8883513
    Abstract: The present invention relates to droplet-based particle sorting. According to one embodiment, a droplet microactuator is provided and includes: (a) a suspension of particles; and (b) electrodes arranged for conducting droplet operations using droplets comprising particles. A method of transporting a particle is also provided, wherein the method includes providing a droplet comprising the particle and transporting the droplet on a droplet microactuator.
    Type: Grant
    Filed: February 18, 2011
    Date of Patent: November 11, 2014
    Assignee: Advanced Liquid Logic, Inc.
    Inventors: Michael G. Pollack, Vamsee Pamula, Vijay Srinivasan
  • Patent number: 8871496
    Abstract: Embodiments of the present invention provide methods, microfluidic devices, and systems for the detection of an active target agent in a fluid sample. A substrate molecule is used that contains a sequence which may cleave in the presence of an active target agent. A SNAP25 sequence is described, for example, that may be cleaved in the presence of Botulinum Neurotoxin. The substrate molecule includes a reporter moiety. The substrate molecule is exposed to the sample, and resulting reaction products separated using electrophoretic separation. The elution time of the reporter moiety may be utilized to identify the presence or absence of the active target agent.
    Type: Grant
    Filed: August 20, 2009
    Date of Patent: October 28, 2014
    Assignee: Sandia Corporation
    Inventors: Gregory J. Sommer, Anson V. Hatch, Anup K. Singh, Ying-Chih Wang
  • Patent number: 8865075
    Abstract: The present invention relates to an immunoaffinity device for capturing one or more analytes present at high or low concentrations in simple or complex matrices. The device is designed as a modular, multi-task immunoaffinity device secured to a peripheral box and connected to capillary electrophoresis or liquid chromatography for the isolation, enrichment, separation and identification of polymeric macromolecules, primarily protein biomarkers. In one embodiment, two devices are coupled in-tandem to perform separately and sequentially microreactions and concentrations of proteins. In this embodiment, biological samples containing proteins can be subjected to proteolytic processing in the first device, and the resulting peptides subjected to selective purification and concentration in the second device.
    Type: Grant
    Filed: October 28, 2011
    Date of Patent: October 21, 2014
    Assignee: Princeton Biochemicals, Inc.
    Inventor: Norberto A. Guzman
  • Publication number: 20140308163
    Abstract: This disclosure is directed to a device and a system for picking a target analyte of a suspension. A picker introduces at least one force, such as by a magnetic gradient and/or by a pressure gradient, to extract the target analyte from a specimen. The magnetic gradient may be introduced by a magnet, such as a permanent magnet or an electromagnet, and the pressure gradient may be introduced by a pump which moves within a fluid-primed cannula to create the pressure gradient, thereby drawing the target analyte into the cannula. The picker may also expel the target analyte onto or into a substrate, such as a well plate, after the target analyte has been drawn into the picker by reversing the pressure gradient or removing the magnetic gradient.
    Type: Application
    Filed: April 9, 2014
    Publication date: October 16, 2014
    Applicant: RareCyte, Inc.
    Inventors: Joshua Nordberg, Steve Quarre
  • Patent number: 8845965
    Abstract: The present application relates to a sensor (1) for use in detecting an analyte comprising: a transducer (10) composed of a pyroelectric or piezoelectric polymer substrate e.g. PVDF (3) and a transparent electrode layer (4) on a surface of the substrate; a layer of parylene (12) on the transparent electrode layer; and a reagent (17) immobilised on the transducer, the reagent having a binding site which is capable of binding the analyte or a derivative of the analyte.
    Type: Grant
    Filed: March 31, 2009
    Date of Patent: September 30, 2014
    Assignee: Vivacta Ltd.
    Inventors: Timothy Joseph Nicholas Carter, Steven Andrew Ross, Timothy Stuart Dwyer
  • Publication number: 20140273277
    Abstract: An apparatus for measuring the luminescence and the fluorescence of a sample, comprising: a light tight optics box capable of receiving a pipette tip containing a sample; an optical sensor located within the optics box and capable of being disposed in both a luminescence reading position and a fluorescence reading position; an excitation light fiber optic bundle and a sample transmission fiber optic bundle; an excitation light assembly that projects excitation light onto a first terminus end of the excitation light fiber optic bundle; and an in-line filter located along the sample transmission fiber optic bundle.
    Type: Application
    Filed: March 17, 2014
    Publication date: September 18, 2014
    Inventors: Ronald Norman Diamond, Steve Michael Gann, Eric Darnell Hall, Tae Ho Hwang, John Lewis Morton, Anatoly Moskalev, Bruce Alan Sargeant, Dennis Edwin Rieger, Marinela Gombosev, Mark David Van Cleve
  • Patent number: 8828335
    Abstract: Fluid-based no-moving part logic devices are constructed from complex sequences of micro- and nanofluidic channels, on-demand bubble/droplet modulators and generators for programming the devices, and micro- and nanofluidic droplet/bubble memory elements for storage and retrieval of biological or chemical elements. The input sequence of bubbles/droplets encodes information, with the output being another sequence of bubbles/droplets or on-chip chemical synthesis. For performing a set of reactions/tasks or process control, the modulators can be used to program the device by producing a precisely timed sequence of bubbles/droplets, resulting in a cascade of logic operations within the micro- or nanofluidic channel sequence, utilizing the generated droplets/bubbles as a control. The devices are based on the principle of minimum energy interfaces formed between the two fluid phases enclosed inside precise channel geometries.
    Type: Grant
    Filed: February 26, 2013
    Date of Patent: September 9, 2014
    Assignee: Massachusetts Institute of Technology
    Inventors: Manu Prakash, Neil Gershenfeld
  • Patent number: 8831783
    Abstract: A biochemical processing apparatus is provided having a stage receiving a biochemical reaction cartridge which includes chambers and flow paths communicating therebetween, a moving system for moving liquid via the flow paths, and a detector for detecting the presence of the liquid in a chamber and/or the amount of the liquid. In addition, a determining device determines a result of the movement of the liquid from the information of the liquid in the chamber detected by the detector.
    Type: Grant
    Filed: August 24, 2011
    Date of Patent: September 9, 2014
    Assignee: Canon Kabushiki Kaisha
    Inventor: Yasuyuki Numajiri
  • Patent number: 8815070
    Abstract: Disclosed herein is a device A device of the microelectrode array architecture, comprising: (a) a bottom plate comprising an array of multiple microelectrodes disposed on a top surface of a substrate covered by a dielectric layer; wherein each of the microelectrode is coupled to at least one grounding elements of a grounding mechanism, wherein a hydrophobic layer is disposed on the top of the dielectric layer and the grounding elements to make hydrophobic surfaces with the droplets; (b) a field programmability mechanism for programming a group of configured-electrodes to generate microfluidic components and layouts with selected shapes and sizes; and, (c) a system management unit, comprising: (i) a droplet manipulation unit; and (ii) a system control unit.
    Type: Grant
    Filed: February 17, 2011
    Date of Patent: August 26, 2014
    Assignee: Sparkle Power, Inc.
    Inventors: Gary Chorng-Jyh Wang, Ching Yen Ho, Wen Jang Hwang, Wilson Wen-Fu Wang
  • Patent number: 8808624
    Abstract: This blood analyzer includes a sample preparation portion preparing a measurement sample free from a labeling substance from a blood sample and a hemolytic agent free from a labeling substance, a light information generation portion generating fluorescent information and at least two types of scattered light information from the measurement sample and a control portion performing a first classification of white blood cells in the measurement sample into at least four groups of monocytes, neutrophils, eosinophils and others on the basis of the fluorescent information and the two types of scattered light information.
    Type: Grant
    Filed: November 9, 2010
    Date of Patent: August 19, 2014
    Assignee: Sysmex Corporation
    Inventors: Hideaki Matsumoto, Kinya Uchihashi, Yuji Itose, Aya Konishi
  • Patent number: 8808625
    Abstract: In a dispensing apparatus for dispensing a liquid, such as a sample solution, on a substrate, such as a glass slide, the dispensing apparatus includes a capillary provided with a distal end and a proximal end, a pump unit configured to pump an operating liquid into the capillary and to pump the operating liquid out of the capillary, and a controller configured to control the pump unit so as to change a position of a liquid surface of the operating liquid in the capillary so that a predetermined volume of liquid is suctioned from the distal end into the capillary and the liquid suctioned in the capillary is discharged from the distal end. As a result, it is possible to precisely dispense extremely small volume amounts of liquids such as a nanoliter.
    Type: Grant
    Filed: August 29, 2008
    Date of Patent: August 19, 2014
    Assignee: National Institute of Advanced Industrial Science and Technology
    Inventors: Hiroshi Aoki, Hiroaki Tao, Masaki Torimura, Takashi Ikeda
  • Patent number: 8802028
    Abstract: A fluid sample collection device for a disk-based fluid separation system is disclosed. The disk-based separation system includes a compact microfluidic disk with at least one flow channel pattern formed on a side surface of the disk. At least one orifice is formed on an outflow boundary of the disk and is designed in fluid communication with the flow channel pattern through a communication channel. The fluid sample collection device includes at least one collection tube having an open end serving as a fluid receiving end and corresponding to the orifice of the disk with a distance. When the disk is rotated, at least a portion of fluid sample in a sample processing reservoir formed on the disk is delivered by centripetal force through the communication channel and the orifice, and finally the expelling fluid sample is collected in the collection tube.
    Type: Grant
    Filed: June 28, 2013
    Date of Patent: August 12, 2014
    Assignee: National Taiwan University
    Inventors: Andrew Man Chung Wo, Chen-Lin Chen, Cheng-Wei Yang, Yu-Cheng Pan
  • Patent number: 8802029
    Abstract: Systems and methods for improved measurement of absorbance/transmission through fluidic systems are described. Specifically, in one set of embodiments, optical elements are fabricated on one side of a transparent fluidic device opposite a series of fluidic channels. The optical elements may guide incident light passing through the device such that most of the light is dispersed away from specific areas of the device, such as intervening portions between the fluidic channels. By decreasing the amount of light incident upon these intervening portions, the amount of noise in the detection signal can be decreased when using certain optical detection systems.
    Type: Grant
    Filed: May 20, 2013
    Date of Patent: August 12, 2014
    Assignee: OPKO Diagnostics, LLC
    Inventors: David Steinmiller, Vincent Linder
  • Publication number: 20140220693
    Abstract: A plunger 66 is moved downwardly in predetermined distance while the tip of the sample probe 15 is immersed in a sample to suck the sample into the probe. A pressure sensor 26 detects the pressure fluctuation during the suction operation, an AD converter converting the signals into digital signals to send the signals for a signal processing unit 76. The signal processing unit 76 extracts feature variables data of a suction waveform to calculate the Statistical distance D from normal group data. The Statistical distance D an a threshold value th are compared with each other, it is judged that there is an abnormality in the suction operation when the Statistical distance D is more than or equal to the threshold value th. When the Statistical distance D is smaller than the threshold value th, an operation is proceeded to a discharge operation.
    Type: Application
    Filed: July 19, 2012
    Publication date: August 7, 2014
    Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION
    Inventors: Isao Yamazaki, Masaharu Nishida, Kumiko Kamihara, Hideto Tamezane
  • Patent number: 8771611
    Abstract: Devices and methods use an integrated microfluidic system that has the capability of realizing a wide range of accurate dilutions in a logarithmic way through semi-direct dilution of samples inside a chip. The device for dose response analysis is able to contain a first fluid source on a microfluidic chip, wherein the first fluid source comprises a drug, a second fluid source on the microfluidic chip, a mixing area on the microfluidic chip fluidically coupling with the first and the second fluidic source, and a detection area coupling with the mixing area for drug information detection using a detection system.
    Type: Grant
    Filed: November 28, 2011
    Date of Patent: July 8, 2014
    Assignees: Auburn University, Pukyong National University
    Inventors: Jong Wook Hong, Se-Kwon Kim, Jae Young Yun
  • Publication number: 20140178251
    Abstract: A sample analyzer comprises a container setting part on which a liquid container is to be set, a liquid supplying part configured to supply a liquid to the liquid container, an aspiration tube configured to aspirate a sample or a reagent, a movement mechanism configured to move the aspiration tube, a liquid surface sensor configured to detect contact of the aspiration tube with a liquid surface, and a controller configured to execute an aspiration tube adjustment operation. The aspiration tube adjustment operation comprises supplying the liquid to the liquid container by the liquid supplying part, lowering the aspiration tube by the movement mechanism toward the liquid container set on the container setting part, and obtaining information regarding a position in a height direction of the aspiration tube at a time when the aspiration tube has come into contact with the liquid surface.
    Type: Application
    Filed: December 19, 2013
    Publication date: June 26, 2014
    Applicant: Sysmex Corporation
    Inventors: Miyuki YAMADA, Shuji KAWAGUCHI, Hiroshi KURONO
  • Patent number: 8753894
    Abstract: An integrated microelectronic sensor is provided in a disposable flow membrane sensing device. The integrated sensors detect electromagnetic effect labels in flow detection zones above the sensor in the membrane. The labels are small particles that give off a detectable electromagnetic signal. They are commonly used for isolating and quantifying biochemical targets of interest. The sensors are fabricated using planar integrated circuit technologies. Sensors can detect labels of several types including magnetic, electric, and photonic. These types all have in common the fact that the sensor detects the label at a distance. Magnetoresistive sensors for detecting magnetic labels, and photodiodes for detecting photonic labels are described. A system for using the sensors is described. There are disposable cartridges with a backing that supports the sensors and membrane is described. The integrated sensor in the cartridge is designed to be discarded after use. Also, label excitation sources are provided.
    Type: Grant
    Filed: February 1, 2008
    Date of Patent: June 17, 2014
    Assignee: Diagnostic Biosensors, LLC
    Inventor: Mark Christopher Tondra
  • Patent number: 8748165
    Abstract: This invention provides a method for generating short tandem repeat (STR) profiles on each of a plurality of samples comprising, for each sample: a) isolating DNA from the sample; b) amplifying STR markers in the isolated DNA and c) analyzing the amplification product by electrophoresis.
    Type: Grant
    Filed: August 21, 2012
    Date of Patent: June 10, 2014
    Assignee: IntegenX Inc.
    Inventors: Mattias Vangbo, William D. Nielsen, Iuliu I. Blaga, Michael Van Nguyen, Steven B. Jovanovich
  • Publication number: 20140141523
    Abstract: A use composition monitor determines the concentration of peracid and/or peroxide in a use composition using a kinetic assay procedure. A sample mixture containing a sample of the use composition, a diluent and at least one reagent is prepared and analyzed using, for example, an optical detector. A reduced-turbulence optical detector can be used to improve collected response data. A reduced-turbulence optical detector can include a cell body disposed about a length of transparent tubing. The cell body positions one or more emitter/receiver pairs about the transparent tubing. Thus, tube junctions are eliminated and sample flow within the tube is substantially turbulence free.
    Type: Application
    Filed: October 11, 2013
    Publication date: May 22, 2014
    Inventors: Eugene Tokhtuev, Christopher Owen, Anna Pilipchenko, Paul Schilling, Daniel Kamben
  • Patent number: 8721966
    Abstract: A method for dispensing a sample solution and a reagent into a container in a chemical analyzer includes a first step of dispensing a reagent into a container, a second step of dispensing a sample solution into the container after the first step, and a third step of dispensing the reagent into the container after the second step. A dilution cup includes an inner face where a diameter of a horizontal section increases on at least one position from a bottom to a top, an opening portion surrounding an opening, where a sample solution is dispensed, located on or near the top, a reagent aperture portion surrounding an aperture where a reagent is dispensed such that the sample solution and the reagent create an upswing spiral flow, and a drain aperture portion surrounding a drain aperture, where a mixed solution of the sample solution and the reagent is discharged, located on or near the bottom.
    Type: Grant
    Filed: March 3, 2010
    Date of Patent: May 13, 2014
    Assignees: Kabushiki Kaisha Toshiba, Toshiba Medical Systems Corporation
    Inventors: Hitoshi Shibutani, Naoto Sato