And Means Segmenting Fluid Material Patents (Class 422/82)
  • Patent number: 10253357
    Abstract: Colorimetry is used to detect amplification reaction products. A sample is contacted with a reaction mix under conditions such that an amplification reaction occurs and produces an amplification reaction product if the sample contains a target nucleic acid template molecule. The reaction mix includes an enzyme for catalyzing the amplification reaction, and at least one halochromic agent. If the target nucleic acid template molecule is present, the amplification reaction changes the starting pH of the reaction mix to cause a detectable colorimetric change of the halochromic agent, thereby indicating the presence of the target nucleic acid. If the target nucleic acid template molecule is not present, the amplification reaction does not generate an adequate number of protons to sufficiently change the starting pH of the reaction mix to cause a detectable colorimetric change of the halochromic agent, thereby indicating that the amplification reaction product has not been produced.
    Type: Grant
    Filed: April 24, 2015
    Date of Patent: April 9, 2019
    Assignee: DIASSESS INC.
    Inventors: Debkishore Mitra, Ivan Krastev Dimov, John Robert Waldeisen
  • Patent number: 9994713
    Abstract: Fluorescent Schiff base compounds comprising a xanthene dye based moiety and an alkyl imidazole moiety. Methods of assessing these fluorescent Schiff base compounds as fluorescent probes for metal ions in Cu2+ chemosensing applications, methods of preparing the fluorescent Schiff base compounds and methods of detecting Cu2+ ions with the same are also provided.
    Type: Grant
    Filed: March 28, 2016
    Date of Patent: June 12, 2018
    Assignee: King Fahd University of Petroleum and Minerals
    Inventor: Aasif Helal
  • Patent number: 9713802
    Abstract: The present invention provides microsphere manufacturing method and apparatus capable of stable obtaining microspheres of a desired size and reducing facility cost. According to the method and the apparatus, to manufacture microspheres made of a second liquid in a first liquid, the first liquid is supplied into a first channel to flow therein and the second liquid is supplied to an intermediate part of the first channel through a second channel. The supply velocities of the first and second liquids are set such that the second liquid closes the first channel and the closing part of the second liquid is cut off due to a pressure difference between an upstream side and a downstream side to form microspheres.
    Type: Grant
    Filed: May 28, 2009
    Date of Patent: July 25, 2017
    Assignee: Kobe Steel, Ltd.
    Inventors: Hiroyuki Ban, Koji Noishiki, Kazuto Okada, Seiichi Yamamoto
  • Patent number: 9120032
    Abstract: Provided is a microchip capable of integrating liquid evaporation as an operation on the microchip. In the microchip 10 having a gas flow path 13 inside, liquid is dispersed by capillary action and pooled in a pool portion 12 at a bottom of the gas flow path 13, and at least a part of the liquid pooled in the pool portion 12 is evaporated. As the capillary action is used, the liquid can be dispersed and pooled in the pool portion 12 at the bottom of the gas flow path 13 inside the microchip 10. Besides, the liquid pooled in the pool portion 12 remains in the pool portion by a surface tension even if gas is made to flow in the gas flow path 13 or the gas flow path is evacuated for evaporation. This enables highly efficient evaporation inside the microchip 10.
    Type: Grant
    Filed: October 30, 2008
    Date of Patent: September 1, 2015
    Assignees: JFE ENGINEERING CORPORATION, KANAGAWA ACADEMY OF SCIENCE AND TECHNOLOGY
    Inventors: Katsuhiko Ohsaki, Shigeki Yamazaki, Takehiko Kitamori, Masaharu Ueno, Kazuma Mawatari, Yoshikuni Kikutani
  • Patent number: 9110049
    Abstract: A use composition monitor determines the concentration of peracid and/or peroxide in a use composition using a kinetic assay procedure. A sample mixture containing a sample of the use composition, a diluent and at least one reagent is prepared and analyzed using, for example, an optical detector. A reduced-turbulence optical detector can be used to improve collected response data. A reduced-turbulence optical detector can include a cell body disposed about a length of transparent tubing. The cell body positions one or more emitter/receiver pairs about the transparent tubing. Thus, tube junctions are eliminated and sample flow within the tube is substantially turbulence free.
    Type: Grant
    Filed: October 11, 2013
    Date of Patent: August 18, 2015
    Assignee: Ecolab USA Inc.
    Inventors: Eugene Tokhtuev, Christopher Owen, Anna Pilipchenko, Paul Schilling, Daniel Kamben
  • Patent number: 9011777
    Abstract: A system for monodispersed microdroplet generation and trapping including providing a flow channel in a microchip; producing microdroplets in the flow channel, the microdroplets movable in the flow channel; providing carrier fluid in the flow channel using a pump or pressure source; controlling movement of the microdroplets in the flow channel and trapping the microdroplets in a desired location in the flow channel. The system includes a microchip; a flow channel in the microchip; a droplet maker that generates microdroplets, the droplet maker connected to the flow channel; a carrier fluid in the flow channel, the carrier fluid introduced to the flow channel by a source of carrier fluid, the source of carrier fluid including a pump or pressure source; a valve connected to the carrier fluid that controls flow of the carrier fluid and enables trapping of the microdroplets.
    Type: Grant
    Filed: February 3, 2009
    Date of Patent: April 21, 2015
    Assignee: Lawrence Livermore National Security, LLC
    Inventor: Neil Reginald Beer
  • Patent number: 9012203
    Abstract: A microfluidic device for controlled encapsulation of particles of sub-millimetric dimensions, or clusters of such particles, the device comprising: a first duct for delivering a first liquid phase containing particles for encapsulating in suspension; a second duct for conveying a flow of a second liquid phase that is immiscible with said first liquid phase; the first duct opening out into the second duct and forming a fluidic junction therewith; at least one microfluidic duct for discharging the first liquid phase flowing in said first duct and provided with a mouth located upstream from said junction and liable to be obstructed, at least in part, by a particle in suspension, thereby causing pressure to rise in the first duct. The invention also provides a microfluidic system including such a device, and a method of encapsulation based on using such a device.
    Type: Grant
    Filed: May 13, 2009
    Date of Patent: April 21, 2015
    Assignee: Commissariat a l'Energie Atomique
    Inventors: Florence Rivera, Jean Berthier, Sophie Le Vot
  • Patent number: 9012206
    Abstract: The invention provides methods of performing a sizing analysis. In the methods, a sizing ladder used in performing the sizing analysis is corrected. In one method, the sizing ladder is corrected for batch-to-batch variations in a sieving gel. In another method, the sizing ladder is corrected for a sample concentration that is different from the archival sizing ladder concentration. Methods are also provided in which the sizing ladder is corrected using a standard marker in a sample and/or using a real-time standard sizing ladder. The methods may be used individually or in combination.
    Type: Grant
    Filed: October 28, 2014
    Date of Patent: April 21, 2015
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Dan Camporese, Advit Bhatt, Josh Molho, Hui Xu, Ken Summers
  • Patent number: 9005545
    Abstract: The present invention relates to a method for detecting the presence and/or the reaction of a biomolecule by monitoring changes of electrical property accurately according to the biological, biochemical or chemical reaction of the biomolecule, and a biochip provided for this purpose.
    Type: Grant
    Filed: December 7, 2012
    Date of Patent: April 14, 2015
    Assignee: Digital Genomics Inc.
    Inventor: Jae-Hoon Lee
  • Patent number: 8986449
    Abstract: The invention relates to a microfluidic device for promoting crystallization of target molecules, such as proteins. The device comprises a solid structure with a top face and an opposite bottom face and with a least one liquid channel. The liquid channel comprises a target molecule solution inlet and at least two precipitant inlets. The target molecule solution inlet is in liquid communication with each of the precipitant inlets through the liquid channel. The liquid channel comprises a branching channel section adjacent to the target molecule solution inlet, crystallization channel sections adjacent to the respective precipitant inlets and flow break channel sections arranged between the branching channel section and each of the crystallization channel sections.
    Type: Grant
    Filed: June 27, 2007
    Date of Patent: March 24, 2015
    Assignee: Microlytic North America Inc.
    Inventor: Morten Sommer
  • Patent number: 8969071
    Abstract: An apparatus for passive sorting of microdroplets including a main flow channel, a flow stream of microdroplets in the main flow channel wherein the microdroplets have substantially the same diameter and wherein the flow stream of microdroplets includes first microdroplets having a first degree of stiffness and second microdroplets having a second degree of stiffness wherein the second degree of stiffness is different than the first degree of stiffness. A second flow channel is connected to the main flow channel for the second microdroplets having a second degree of stiffness. A separator separates the second microdroplets having a second degree of stiffness from the first microdroplets and directs the second microdroplets having a second degree of stiffness into the second flow channel.
    Type: Grant
    Filed: November 3, 2010
    Date of Patent: March 3, 2015
    Assignees: Lawrence Livermore National Security, LLC, The Regents of the University of California
    Inventors: Neil Reginald Beer, Abraham P. Lee, Andrew C. Hatch, Jeffrey S. Fisher
  • Patent number: 8968661
    Abstract: A sample analyzer prepares a measurement sample from a blood sample or a body fluid sample which differs from the blood sample; measures the prepared measurement sample; obtains characteristic information representing characteristics of the components in the measurement sample; sets either a blood measurement mode for measuring the blood sample, or a body fluid measurement mode for measuring the body fluid sample as an operating mode; and measures the measurement sample prepared from the blood sample by executing operations in the blood measurement mode when the blood measurement mode has been set, and measuring the measurement sample prepared from the body fluid sample by executing operations in the body fluid measurement mode that differs from the operations in the blood measurement mode when the body fluid measurement mode has been set, is disclosed. A computer program product is also disclosed.
    Type: Grant
    Filed: May 10, 2013
    Date of Patent: March 3, 2015
    Assignee: Sysmex Corporation
    Inventors: Takaaki Nagai, Noriyuki Narisada, Daigo Fukuma
  • Patent number: 8883513
    Abstract: The present invention relates to droplet-based particle sorting. According to one embodiment, a droplet microactuator is provided and includes: (a) a suspension of particles; and (b) electrodes arranged for conducting droplet operations using droplets comprising particles. A method of transporting a particle is also provided, wherein the method includes providing a droplet comprising the particle and transporting the droplet on a droplet microactuator.
    Type: Grant
    Filed: February 18, 2011
    Date of Patent: November 11, 2014
    Assignee: Advanced Liquid Logic, Inc.
    Inventors: Michael G. Pollack, Vamsee Pamula, Vijay Srinivasan
  • Patent number: 8871496
    Abstract: Embodiments of the present invention provide methods, microfluidic devices, and systems for the detection of an active target agent in a fluid sample. A substrate molecule is used that contains a sequence which may cleave in the presence of an active target agent. A SNAP25 sequence is described, for example, that may be cleaved in the presence of Botulinum Neurotoxin. The substrate molecule includes a reporter moiety. The substrate molecule is exposed to the sample, and resulting reaction products separated using electrophoretic separation. The elution time of the reporter moiety may be utilized to identify the presence or absence of the active target agent.
    Type: Grant
    Filed: August 20, 2009
    Date of Patent: October 28, 2014
    Assignee: Sandia Corporation
    Inventors: Gregory J. Sommer, Anson V. Hatch, Anup K. Singh, Ying-Chih Wang
  • Patent number: 8871524
    Abstract: The invention provides methods of performing a sizing analysis. In the methods, a sizing ladder used in performing the sizing analysis is corrected. In one method, the sizing ladder is corrected for batch-to-batch variations in a sieving gel. In another method, the sizing ladder is corrected for a sample concentration that is different from the archival sizing ladder concentration. Methods are also provided in which the sizing ladder is corrected using a standard marker in a sample and/or using a real-time standard sizing ladder. The methods may be used individually or in combination.
    Type: Grant
    Filed: November 1, 2011
    Date of Patent: October 28, 2014
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Dan Camporese, Advit Bhatt, Josh Molho, Hui Xu, Ken Summers
  • Publication number: 20140273052
    Abstract: A sensor system in a water treatment system has a housing, controller, one or more light sources, one or more sensors and one or more targets having an immobilized reagent thereon. Light source emits light energy into the housing that is incident upon the target with the immobilized reagent and the reagent being in contact with water from the system. The immobilized reagent interacts with a reactant in the water such that the interaction changes the state of the reagent. When energy from the light source is incident on the target with the immobilized reagent the energy shows a change detectable by the sensor such that the changed energy is detectable by and collected at the sensor and data on the energy is communicated to the controller. The data is then correlated as a representation of a desired variable to be measured for the water in the water treatment system.
    Type: Application
    Filed: March 15, 2013
    Publication date: September 18, 2014
    Inventors: Rakesh Reddy, Bruce Johnson, Kevin Doyle
  • Patent number: 8834807
    Abstract: The invention provides devices and methods for isolating one or more sample components of a sample material following separation of the sample material into a plurality of sample components. A device includes a separation channel having a sample loading well. A low-conductivity buffer is disposed in the loading well, the buffer having a conductivity<0.2 mS/cm. In a method, a buffer is loaded into a loading well in fluid communication with a separation channel of a device. A sample material having a conductivity higher than that of the buffer is then loaded into the loading well such that the sample material is disposed beneath the buffer, the buffer disposed over and covering the sample material. The sample material is separated into a plurality of separated components in the separation channel, and a separated component is collected from a collection well disposed in a collection leg of the device.
    Type: Grant
    Filed: November 1, 2011
    Date of Patent: September 16, 2014
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Josh Molho, Hui Xu
  • Patent number: 8828335
    Abstract: Fluid-based no-moving part logic devices are constructed from complex sequences of micro- and nanofluidic channels, on-demand bubble/droplet modulators and generators for programming the devices, and micro- and nanofluidic droplet/bubble memory elements for storage and retrieval of biological or chemical elements. The input sequence of bubbles/droplets encodes information, with the output being another sequence of bubbles/droplets or on-chip chemical synthesis. For performing a set of reactions/tasks or process control, the modulators can be used to program the device by producing a precisely timed sequence of bubbles/droplets, resulting in a cascade of logic operations within the micro- or nanofluidic channel sequence, utilizing the generated droplets/bubbles as a control. The devices are based on the principle of minimum energy interfaces formed between the two fluid phases enclosed inside precise channel geometries.
    Type: Grant
    Filed: February 26, 2013
    Date of Patent: September 9, 2014
    Assignee: Massachusetts Institute of Technology
    Inventors: Manu Prakash, Neil Gershenfeld
  • Patent number: 8826724
    Abstract: Chemical sensors such as carbon dioxide sensors and methods for making such sensors are disclosed. An example carbon dioxide sensor may include a substrate, with a sensing beam supported by the substrate. The sensing beam may be configured to resonant. A sensing layer may be disposed on the sensing beam, wherein the sensing layer may include an amino group and is configured to sense carbon dioxide. In some instances, a reference beam may also be supported by the substrate, and may be configured to resonant. A reference layer may be disposed on the reference beam, wherein the reference layer may includes an amino group that has been poisoned so that it will be substantially non-sensitive to carbon dioxide.
    Type: Grant
    Filed: December 21, 2011
    Date of Patent: September 9, 2014
    Assignee: Honeywell International Inc.
    Inventors: Bogdan Serban, Mihai N. Mihalia, Cornel Cobianu, Viorel Georgel Dumitru, Octavian Buiu
  • Patent number: 8831783
    Abstract: A biochemical processing apparatus is provided having a stage receiving a biochemical reaction cartridge which includes chambers and flow paths communicating therebetween, a moving system for moving liquid via the flow paths, and a detector for detecting the presence of the liquid in a chamber and/or the amount of the liquid. In addition, a determining device determines a result of the movement of the liquid from the information of the liquid in the chamber detected by the detector.
    Type: Grant
    Filed: August 24, 2011
    Date of Patent: September 9, 2014
    Assignee: Canon Kabushiki Kaisha
    Inventor: Yasuyuki Numajiri
  • Patent number: 8809070
    Abstract: A dispensing method which dispenses a first liquid contained in a first vessel which stores the first liquid and a second liquid to introduce the first liquid into a second vessel by using a tube.
    Type: Grant
    Filed: June 14, 2012
    Date of Patent: August 19, 2014
    Assignee: Seiko Epson Corporation
    Inventors: Yuji Saito, Fumio Takagi
  • Patent number: 8771597
    Abstract: The invention relates to an apparatus for the determination of a concentration of a component to be measured in a gas, comprising a light source, a wavelength selection unit, a measurement cuvette, a reference cuvette arranged in the optical beam path in parallel thereto, at least one light receiver and an evaluation unit which determines the concentration from the signals of the light receiver, wherein the gas to be analyzed is supplied to the measurement cuvette, on the one hand, and, on the other hand, to the reference cuvette via an absorption apparatus which includes a substance which completely absorbs the component to be measured. Further, the component to be measured is H2S and a wavelength selection unit is provided for the selection of an absorption wavelength.
    Type: Grant
    Filed: May 15, 2012
    Date of Patent: July 8, 2014
    Assignee: SICK AG
    Inventors: Michael Zochbauer, Carsten Rogge, Dominikus Huttner
  • Patent number: 8753894
    Abstract: An integrated microelectronic sensor is provided in a disposable flow membrane sensing device. The integrated sensors detect electromagnetic effect labels in flow detection zones above the sensor in the membrane. The labels are small particles that give off a detectable electromagnetic signal. They are commonly used for isolating and quantifying biochemical targets of interest. The sensors are fabricated using planar integrated circuit technologies. Sensors can detect labels of several types including magnetic, electric, and photonic. These types all have in common the fact that the sensor detects the label at a distance. Magnetoresistive sensors for detecting magnetic labels, and photodiodes for detecting photonic labels are described. A system for using the sensors is described. There are disposable cartridges with a backing that supports the sensors and membrane is described. The integrated sensor in the cartridge is designed to be discarded after use. Also, label excitation sources are provided.
    Type: Grant
    Filed: February 1, 2008
    Date of Patent: June 17, 2014
    Assignee: Diagnostic Biosensors, LLC
    Inventor: Mark Christopher Tondra
  • Patent number: 8753773
    Abstract: A simple and low cost device to detect the presence of mercury or other trace metals in the environment and quantify said concentration by spectrophotometric, colorimetric, or electrical conductivity is disclosed. Owing to its small size, it is suitable for portable mercury detectors or detectors that may be worn as badges in the breathing zone of workers.
    Type: Grant
    Filed: June 29, 2012
    Date of Patent: June 17, 2014
    Inventors: John Perrin Davis, Cheryl D. Stevenson
  • Patent number: 8748191
    Abstract: Analytical systems and methods are provided for simultaneously dispensing metered volumes of fluids at different rates and mixing the fluids to generate a mixed sample having the fluids in proportion to the different rates at which they were dispensed. In some cases two or more of the fluids are premixed prior to mixing with other fluids. In some cases a use composition and diluent are simultaneously dispensed at different rates and premixed to form a diluted sample. One or more reagents may be mixed with the diluted sample and the sample mixture can be analyzed to determine characteristics of the use composition.
    Type: Grant
    Filed: August 2, 2010
    Date of Patent: June 10, 2014
    Assignee: Ecolab USA Inc.
    Inventors: Paul R. Kraus, William M. Christensen
  • Publication number: 20140148358
    Abstract: Various embodiments provide a system for detecting one or more analytes in a fluid. The system comprises: a controller adapted to obtain one or more sample streams of the fluid. The controller is configured in use to form a plurality of output streams. Each output stream comprises at least part of one of the one or more sample streams. The system further comprises: a detector adapted to receive from the controller the plurality of output streams and comprising a plurality of sensors. Each sensor is operable to detect an interaction between a corresponding detecting agent and a corresponding analyte. The detector is configured in use to detect one or more said interactions using the plurality of output streams to determine if the fluid contains one or more said analytes. A corresponding method is also provided.
    Type: Application
    Filed: June 28, 2012
    Publication date: May 29, 2014
    Applicant: NATIONAL UNIVERSITY OF SINGAPORE
    Inventors: Fong Yau Sam Li, Huanan Wu, Mahe Liu
  • Patent number: 8735169
    Abstract: The present invention generally relates methods for analyzing agricultural and/or environmental samples using liquid bridges. In certain embodiments, the invention provides a method for analyzing an agricultural sample for a desired trait including obtaining a gene or gene product from an agricultural sample, in which the gene or gene product is in a first fluid; providing a liquid bridge for mixing the gene or gene product with at least one reagent to form a mixed droplet that is wrapped in an immiscible second fluid; and analyzing the mixed droplet to detect a desired trait of the agricultural sample.
    Type: Grant
    Filed: May 20, 2009
    Date of Patent: May 27, 2014
    Assignee: Stokes Bio Limited
    Inventors: Mark Davies, Tara Dalton
  • Patent number: 8715592
    Abstract: An integrated electronic-micro fluidic device an integrated electronic-micro fluidic device, comprising a semiconductor substrate on a first support, an electronic circuit on a first semiconductor-substrate side of the semiconductor substrate, and a signal interface structure to an external device. A micro fluidic structure is formed in the semiconductor substrate, and is configured to confine a fluid and to allow a flow of the fluid to and from the microfluidic structure only on a second semiconductor-substrate side that is opposite to the first semiconductor-substrate side and faces away from the first support.
    Type: Grant
    Filed: January 31, 2013
    Date of Patent: May 6, 2014
    Assignee: Koninklijke Philips N.V.
    Inventors: Ronald Dekker, Remco Henricus Wilhelmus Pijnenburg, Nicolaas Johannes Anthonius Van Veen
  • Patent number: 8715573
    Abstract: The fluidic system 10 of the preferred embodiment includes a sheath pump 12 to pump sheath fluid 14 from a sheath container 16 through a sample port 34 into an interrogation zone 18 and a waste pump 20 to pump the sheath fluid 14 and a sample fluid 26 as waste fluid 22 from the interrogation zone 18 into a waste container 24, and a processor 30 to calculate a time window based on the flow rate of the sample fluid 26. Preferably the processor 30 also calculates a time window for the sample fluid to reach the interrogation zone 18 from the sample port 34 based on the flow rate of the sample fluid 26. The interrogation zone 18 functions to provide a location for the fluidic system 10 and an optical analysis system 32 of the flow cytometer to cooperatively facilitate the analysis of the sample fluid 26.
    Type: Grant
    Filed: October 15, 2007
    Date of Patent: May 6, 2014
    Assignee: Accuri Cytometers, Inc.
    Inventors: Jack T. Ball, Collin A. Rich
  • Patent number: 8703069
    Abstract: This disclosure provides systems, methods, and devices for processing samples on a microfluidic device. One method includes moving a sample from an upstream channel of a microfluidic device into a DNA manipulation module located downstream of the upstream channel. The DNA manipulation module includes a DNA manipulation zone configured to perform amplification of the sample, a first valve disposed upstream of the DNA manipulation zone, and a second valve disposed downstream of the DNA manipulation zone. The method also includes receiving the sample in the DNA manipulation zone; closing the first valve and the second valve such that as and liquid are prevented from flowing into or out of the DNA manipulation zone; and thermal cycling the sample in the DNA manipulation zone.
    Type: Grant
    Filed: September 14, 2012
    Date of Patent: April 22, 2014
    Assignee: Handylab, Inc.
    Inventors: Kalyan Handique, Gene Parunak
  • Patent number: 8704158
    Abstract: The invention concerns fluorescence standards, and in particular fluorescence standards for calibrating optical detectors. According to the invention, a fluorescent mineral or mixtures of minerals are employed for use as a fluorescence standard. The fluorescent mineral can be a naturally occurring mineral or a synthetically produced mineral. Preferred fluorescent minerals for use as fluorescence standards are corundum, fluorite, turquoise, amber, zircon, zoisite, iolite or cordierite, spinel, topaz, calcium fluorite, sphalerite or zincblende, calcite or calcspar, apatite, scheelite or calcium tungstate, willemite, feldspars, sodalite, a uranium mineral, a mineral containing Al3+, and in particular ruby and sapphire.
    Type: Grant
    Filed: April 17, 2009
    Date of Patent: April 22, 2014
    Assignee: Qiagen Lake Constance GmbH
    Inventors: Klaus Haberstroh, Konrad Faulstich
  • Patent number: 8704977
    Abstract: The invention provides a liquid crystal cell and method thereof. The cell comprises two opposed substrates and a surfactant-free lyotropic chromonic liquid crystals (LCLC) material disposed therebetween. By using an ammonium compound with LCLC or surface treatment on the substrates, the alignment of the LCLC material can be manipulated as a homeotropic bulk alignment; or a hybrid bulk alignment in which the LCLC alignment is changed from homeotropic bulk alignment in the vicinity of one substrate to planar alignment in the vicinity of another substrate. The cell can be used in biosensing, detection and amplification of ligands, optical devices, and photovoltaics etc.
    Type: Grant
    Filed: March 5, 2008
    Date of Patent: April 22, 2014
    Assignee: Kent State University
    Inventors: Oleg D. Lavrentovich, Yuriy A. Nastyshyn, Vassili G. Nazarenko, Roman M. Vasyuta, Oleksandr P. Boiko, Ye Yin, Sergij V. Shiyanovskii
  • Patent number: 8658367
    Abstract: A microfluidic device is provided for analyzing or sorting biological materials, such as polynucleotides, polypeptides, proteins, enzymes, viruses and cells. The invention can be used for high throughput or combinatorial screening. The device comprises a main channel and an inlet channel that communicate at a droplet extrusion region so that droplets of solution are deposited into an immiscible solvent in the main channel. Droplets can thereafter be sorted according to biological material detected in each droplet.
    Type: Grant
    Filed: May 18, 2012
    Date of Patent: February 25, 2014
    Assignee: California Institute of Technology
    Inventors: Stephen R. Quake, Todd Thorsen
  • Patent number: 8658368
    Abstract: A microfluidic device is provided for analyzing or sorting biological materials, such as polynucleotides, polypeptides, proteins, enzymes, viruses and cells. The invention can be used for high throughput or combinatorial screening. The device comprises a main channel and an inlet channel that communicate at a droplet extrusion region so that droplets of solution are deposited into an immiscible solvent in the main channel. Droplets can thereafter be sorted according to biological material detected in each droplet.
    Type: Grant
    Filed: May 18, 2012
    Date of Patent: February 25, 2014
    Assignee: California Institute of Technology
    Inventors: Stephen R. Quake, Todd Thorsen
  • Patent number: 8637261
    Abstract: The present invention, provides a flow cytometry apparatus for the detection of particles from a plurality of samples comprising: means for moving a plurality of samples comprising particles from a plurality of respective source wells into a fluid flow stream; means for introducing a separation gas between each of the plurality of samples in the fluid flow stream; and means for selectively analyzing each of the plurality of samples for the particles. The present invention also provides a flow cytometry method employing such an apparatus.
    Type: Grant
    Filed: August 14, 2012
    Date of Patent: January 28, 2014
    Assignee: STC.UNM
    Inventors: Larry A Sklar, Bruce Edwards, Frederick Kuckuck
  • Patent number: 8632734
    Abstract: A microreaction device or system (4) includes at least one thermal control fluidic passage (C,E) and a principal working fluidic passage (A) with average cross-sectional area in the range of 0.25 to 100 mm2, and having a primary entrance (92) and multiple secondary entrances (94) with the spacing between secondary entrances (94) having a length along the passage (A) of at least two times the root of the average cross-sectional area of the passage (A). The device or system (4) also includes at least one secondary working fluidic passage (B) having an entrance (102) and multiple exits (106) including a final exit (106), each exit (106) being in fluid communication with a corresponding one of the multiple secondary entrances (94) of the principal fluidic passage (A).
    Type: Grant
    Filed: May 7, 2007
    Date of Patent: January 21, 2014
    Assignee: Corning Incorporated
    Inventors: Philippe J Barthe, Nikolaus Bieler, Celine Claude Guermeur, Olivier Lobet, Maxime Moreno, Dominique Roberge, Pierre Woehl
  • Patent number: 8613890
    Abstract: Disclosed herein is a microparticle sorting apparatus, including: a discharge direction confirming section configured to confirm a discharge direction of a discharged liquid discharged from a discharge outlet of a flow path; opposite electrodes disposed downstream with respect to the discharge outlet; an opposite electrode position controlling section configured to control positions of the opposite electrodes in accordance with the discharge direction confirmed by the discharge direction confirming section; a droplet generating section configured to generate a droplet from the discharge outlet; and a charging section configured to electrically charge the droplet with electric charges.
    Type: Grant
    Filed: July 30, 2010
    Date of Patent: December 24, 2013
    Assignee: Sony Corporation
    Inventor: Yosuke Muraki
  • Patent number: 8613889
    Abstract: The present invention relates to droplet-based washing. According to one embodiment, a method of providing a droplet in contact with a surface with a reduced concentration of a substance is provided, wherein the method includes: (a) providing a surface in contact with a droplet comprising a starting concentration and starting quantity of the substance and having a starting volume; (b) conducting one or more droplet operations to merge a wash droplet with the droplet provided in step (a) to yield a combined droplet; and (c) conducting one or more droplet operations to divide the combined droplet to yield a set of droplets comprising: (i) a droplet in contact with the surface having a decreased concentration of the substance relative to the starting concentration; and (ii) a droplet which is separated from the surface.
    Type: Grant
    Filed: December 15, 2006
    Date of Patent: December 24, 2013
    Assignees: Advanced Liquid Logic, Inc., Duke University
    Inventors: Michael G. Pollack, Vamsee K. Pamula, Vijay Srinivasan, Richard B. Fair
  • Patent number: 8592215
    Abstract: This invention provides microfabricated devices and methods for detecting, analyzing and sorting biological materials and particles. Droplets containing the particles are provided in an extrusion fluid, passed through a detection region, and then directed into a branch channel according to predetermined characteristics. For example, cells or viral particles contained in droplets of aqueous solvent are flowed past a detector in the nonpolar extrusion fluid decane, and routed into a selected branch channel for subsequent analysis or use.
    Type: Grant
    Filed: September 29, 2011
    Date of Patent: November 26, 2013
    Assignee: California Institute of Technology
    Inventors: Stephen R. Quake, Todd Thorsen
  • Patent number: 8584345
    Abstract: Because reprocessing or refurbishing of physiological sensors reuses large portions of an existing sensor, the material costs for refurbishing sensors is significantly lower than the material costs for making an entirely new sensor. Typically, existing reprocessors replace only the adhesive portion of an adhesive physiological sensor and reuse the sensing components. However, re-using the sensing components can reduce the reliability of the refurbished sensor and/or reduce the number of sensors eligible for refurbishing due to out-of-specification sensor components. It is therefore desirable to provide a process for refurbishing physiological sensors that replaces the sensing components of the sensor. While sensing components are replaced, generally, sensor cable and/or patient monitor attachments are retained, resulting in cost savings over producing new sensors.
    Type: Grant
    Filed: March 7, 2011
    Date of Patent: November 19, 2013
    Assignee: Masimo Corporation
    Inventors: Ammar Al-Ali, Yassir Abdul-Hafiz, Massi Joe E. Kiani
  • Patent number: 8563320
    Abstract: A use composition monitor determines the concentration of peracid and/or peroxide in a use composition using a kinetic assay procedure. A sample mixture containing a sample of the use composition, a diluent and at least one reagent is prepared and analyzed using, for example, an optical detector. A reduced-turbulence optical detector can be used to improve collected response data. A reduced-turbulence optical detector can include a cell body disposed about a length of transparent tubing. The cell body positions one or more emitter/receiver pairs about the transparent tubing. Thus, tube junctions are eliminated and sample flow within the tube is substantially turbulence free.
    Type: Grant
    Filed: February 15, 2012
    Date of Patent: October 22, 2013
    Assignee: Ecolab USA Inc.
    Inventors: Eugene Tokhtuev, Christopher Owen, Anna Pilipchenko, Paul Schilling, Daniel Kamben
  • Patent number: 8557598
    Abstract: The present invention relates to a method for measuring a blood sample comprising the steps of: communicating the inside of a sealed container with atmosphere by piercing a cap for sealing the sealed container; supplying a diluted solution from the base end side of the aspiration pipe to fill the aspiration pipe with the diluted solution; aspirating the blood sample in the sealed container; discharging the aspirated blood sample into a measurement sample preparing container; and measuring a measurement sample prepared in the measurement sample preparing container.
    Type: Grant
    Filed: March 17, 2006
    Date of Patent: October 15, 2013
    Assignee: Sysmex Corporation
    Inventors: Takaaki Nagai, Masaharu Shibata
  • Patent number: 8545758
    Abstract: Disclosed herein is a microparticle sorting apparatus, including: a discharge direction confirming section configured to confirm a discharge direction of a discharged liquid discharged from a discharge outlet of a flow path; opposite electrodes disposed downstream with respect to the discharge outlet; an opposite electrode position controlling section configured to control positions of the opposite electrodes in accordance with the discharge direction confirmed by the discharge direction confirming section; a droplet generating section configured to generate a droplet from the discharge outlet; and a charging section configured to electrically charge the droplet with electric charges.
    Type: Grant
    Filed: July 30, 2010
    Date of Patent: October 1, 2013
    Assignee: Sony Corporation
    Inventor: Yosuke Muraki
  • Patent number: 8524506
    Abstract: Methods for sampling a liquid flow are provided. The method includes supplying a continuous liquid flow from a continuous flow fluid input source to a surface along a first flow path. The method additionally includes sampling the continuous liquid flow by performing an electric field-based technique to split off a sample droplet from a portion of the liquid flow, whereby the sample droplet is distinct from the liquid flow and controllable independently of the liquid flow, wherein the electric field-based technique is performed by providing a set of electrodes on the surface and selectively biasing the set of electrodes, whereby the sample droplet is formed on one of the set of electrodes.
    Type: Grant
    Filed: November 7, 2007
    Date of Patent: September 3, 2013
    Assignee: Duke University
    Inventors: Vamsee K. Pamula, Michael G. Pollack, Richard B. Fair
  • Patent number: 8478445
    Abstract: Various embodiments of the present invention provide, for example, a system and method for automatically adjusting the inoculum level of a sample. Certain embodiments of the present invention may measure a concentration of particles present in a preliminary sample using a sensor device and determine an amount of diluent to be added to or removed from a sample container to prepare a sample having a selected concentration of particles, corresponding to a selected inoculum level. Embodiments of the present invention may also automatically add or remove the diluent using an automated fluidics system so as to prepare a sample having the selected particle concentration. Once the selected particle concentration is achieved and verified, some embodiments may also remove at least a portion of the sample from the sample container such that the container contains a selected volume of the sample.
    Type: Grant
    Filed: October 17, 2011
    Date of Patent: July 2, 2013
    Assignee: Becton, Dickinson and Company
    Inventors: Timothy Roy Hansen, Mark Anthony Messina, John Thulin Page, Thomas Paul Borgoyn, Ammon David Lentz, Robert Michael Novak
  • Patent number: 8475715
    Abstract: A system having reduced gas interference that includes a fluid chamber and a resonant sensor device in fluid communication with a fluid in the fluid chamber. The system includes a fluid control device adapted to change at least one of the fluid flow or pressure within the fluid chamber to achieve substantial wetting of surfaces in proximity to the resonant sensor device. Fluid surfaces of the system can include a material to increase the wettability (e.g., hydrophilicity) of the fluid surfaces.
    Type: Grant
    Filed: August 10, 2006
    Date of Patent: July 2, 2013
    Assignee: BioScale, Inc.
    Inventors: Brett P. Masters, Michael F. Miller
  • Patent number: 8465697
    Abstract: Disclosed are a system and method for regulating flow in an exemplary fluidic device comprising a fluidic stream carrying a transport medium, sample and one or more reagents for analysis and synthesis of reaction products. The flow rate of the fluidic stream is maintained constant by adjusting the flow rate of transport medium to compensate for the introduction of sample and reagents. An embodiment controls the flow rate of transport medium using a pump, a back pressure regulator, and a variable-sized orifice. Single and multiple channel embodiments are disclosed.
    Type: Grant
    Filed: December 31, 2008
    Date of Patent: June 18, 2013
    Assignee: O.I. Corporation
    Inventors: Gary L. Erickson, Craig Ranger
  • Patent number: 8464076
    Abstract: A device for generating a born encrypted optical file includes a photovoltaic matrix for converting an optical image into a digital file. The digital file is a collection of digital data that has not been processed by any image processing logic and thus cannot be used to directly generate a reproduced image of the object. An encryption logic converts the digital file into an encrypted digital file that can be exported from the device to an authorized device to create a decrypted digital file. This decrypted digital file is capable of being used by a display logic to display an image of the object.
    Type: Grant
    Filed: March 24, 2011
    Date of Patent: June 11, 2013
    Assignee: International Business Machines Corporation
    Inventors: Timothy Durniak, Robert R. Friedlander, James R. Kraemer
  • Patent number: 8425862
    Abstract: The system has a plurality of dishes (1) for receiving at least one sample, each dish having a perforated base (2) enabling liquid reagents or solutions to pass continuously therethrough, the dishes being superposed in a confining tube (5); a circuit (50) for the continuous outflow of the solutions originating from distinct reservoirs (17); a multipath electrovalve (15); an electric pump (13); and a member for controlling and commanding the pump (13) and the multipath electrovalve (15) in a sequential and coordinated manner.
    Type: Grant
    Filed: March 4, 2004
    Date of Patent: April 23, 2013
    Assignees: Centre National de la Recherche Scientifique, Universite Joseph Fourier
    Inventor: Jacques Lucien Thelu
  • Patent number: 8420397
    Abstract: Fluid flow devices include a small plate (2), at least one flow channel (20) formed into this small plate, at least one storage channel (221-226) extending from this connection channel, and a set of valves (V1-V6), each of which is suitable for allowing or stopping the flow of fluid in a corresponding storage channel.
    Type: Grant
    Filed: October 12, 2007
    Date of Patent: April 16, 2013
    Assignees: Rhodia Operations, Centre National de la Recherche Scientifique (C.N.R.S.)
    Inventors: Mathieu Joanicot, Philippe Laval, Jean-Baptiste Salmon