Methionine; Cysteine; Cystine Patents (Class 435/113)
  • Patent number: 10240140
    Abstract: A gene expression cassette capable of producing psicose at high yield with high stability, a GRAS (Generally recognized as safe) microorganism, a method of producing the enzyme by using the GRAS microorganism, and a method of producing the psicose by using the GRAS microorganism and enzyme are provided.
    Type: Grant
    Filed: October 30, 2015
    Date of Patent: March 26, 2019
    Assignee: SAMYANG CORPORATION
    Inventors: Jin Sol Heo, Hye Jung Kim, Min Jeong Kim, Jeong Yoon Choi, Chong Jin Park, Kang Pyo Lee
  • Patent number: 10221434
    Abstract: The present invention relates to a polypeptide which is resistant to feedback inhibition by methionine and has an activity of homoserine O-succinyltransferase, a microorganism for producing O-succinylhomoserine which expresses the polypeptide, and a method for producing O-succinylhomoserine using the same.
    Type: Grant
    Filed: October 22, 2014
    Date of Patent: March 5, 2019
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Chang Il Seo, So Young Kim, Yong Uk Shin, Kwang Ho Na, Hye Won Um, In Kyung Heo
  • Patent number: 9657328
    Abstract: The present invention relates to a method for modulating thermostability or acid tolerance of a microbe by mutating a nucleic acid molecule encoding a homoserine o-succinyltransferase (MetA) to obtain a nucleic acid molecule encoding a mutant MetA and transforming a microbial cell with the nucleic acid molecule encoding a mutant MetA. The microbe, a bacteria expressing the mutant MetA of the present invention represents a growth rate improved in a vigorous environment such as higher-temperatures and/or lower pH conditions.
    Type: Grant
    Filed: September 11, 2009
    Date of Patent: May 23, 2017
    Assignee: Korean Research Institute of Bioscience and Biotechnology
    Inventors: Jae-Gu Pan, Mor Dukhova Elena Anatolievna
  • Patent number: 9611461
    Abstract: L-cysteine can be produced inexpensively and efficiently by using a bacterium belonging to the family Enterobacteriaceae modified to reduce activity of O-acetylserine sulfhydrylase B thereof, the bacterium being modified so that the C terminal region of its thiosulfate-binding protein is deleted, and the bacterium having an increased ability to produce L-cysteine in the presence of a sulfate.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: April 4, 2017
    Assignee: National University Corporation Nara Institute of Science and Technology
    Inventors: Iwao Ohtsu, Takeshi Nakatani, Ai Tamakoshi, Sayo Takahashi, Hiroshi Takagi
  • Patent number: 9506093
    Abstract: The present invention is related to a recombinant microorganism optimized for the fermentative production of methionine, wherein the activity of the cobalamin-independent methionine synthase MetE is attenuated in said microorganism. The invention is also related to a method for producing methionine by fermentation.
    Type: Grant
    Filed: June 18, 2012
    Date of Patent: November 29, 2016
    Assignee: METABOLIC EXPLORER
    Inventors: Wanda Dischert, Rainer Figge
  • Patent number: 9493801
    Abstract: Described herein are microorganisms that produce methionine and related products from endogenous genes in a transsulfuration pathway, as well as from exogenous genes providing a direct sulfhydrylation pathway. Novel genes that are useful for methionine and SAMe production are disclosed.
    Type: Grant
    Filed: August 6, 2015
    Date of Patent: November 15, 2016
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Brian Brazeau, Jin-Sook Chang, Kwang Myung Cho, Young Wook Cho, Mervyn Desouza, Holly J. Jessen, So-Young Kim, Wei Niu, Fernando A. Sanchez-Riera, Yong-Uk Shin, Hyewon Um
  • Patent number: 9382561
    Abstract: The present invention relates to a method for the production of methionine using modified strains with attenuated transformation of threonine. This can be achieved by reducing threonine transformation into glycine, and/or by reducing its transformation to ?-ketobutyrate. The invention also concerns the modified strains with attenuated transformation of threonine.
    Type: Grant
    Filed: December 29, 2010
    Date of Patent: July 5, 2016
    Assignee: METABOLIC EXPLORER
    Inventors: Cedric Boisart, Gwenaelle Bestel-Corre, Guillaume Barbier, Rainer Figge
  • Patent number: 9347078
    Abstract: The invention relates to a method for producing L-cysteine and its derivatives L-cystine and thiazolidine by fermenting a cysteine-producing microorganism strain in a fermentation medium. The invention is characterized in that L-methionine, L-isoleucine, or L-threonine in respective concentrations ranging from 0.1 to 10 g/L are added to the fermentation medium in the main culture.
    Type: Grant
    Filed: September 9, 2013
    Date of Patent: May 24, 2016
    Assignee: Wacker Chemie AG
    Inventors: Tobias Dassler, Walfred Leinfelder, Guenter Wich
  • Patent number: 9234223
    Abstract: A method for producing L-cysteine and the like is provided by developing a novel technique for improving bacterial L-cysteine-producing ability. The method includes the steps of culturing a bacterium belonging to the genus Escherichia which has L-cysteine-producing ability, and in which expression of a gene involved in sulfite reduction is enhanced, in a medium containing thiosulfate, and collecting L-cysteine, a related substance thereof, or a mixture thereof which accumulate in the medium.
    Type: Grant
    Filed: March 27, 2012
    Date of Patent: January 12, 2016
    Assignee: AJINOMOTO CO., INC.
    Inventors: Shunsuke Yamazaki, Gen Nonaka, Kazuhiro Takumi
  • Patent number: 9187775
    Abstract: A method of producing methionine, derivatives or precursors thereof includes culturing a modified microorganism in a culture medium comprising a source of carbon and a source of sulfur; and recovering methionine from the culture medium, wherein said modified microorganism has an increased expression of cysE gene encoding serine acetyltransferase, metH gene encoding methionine synthase and metF gene encoding 5,10-methylenetetrahydrofolate reductase compared to expression of the cysE, metH and metF genes in an unmodified microorganism.
    Type: Grant
    Filed: January 9, 2013
    Date of Patent: November 17, 2015
    Assignee: Metabolic Explorer
    Inventors: Rainer Figge, Fabien Lux, Céline Raynaud, Michel Chateau, Philippe Soucaille
  • Patent number: 9127257
    Abstract: The present invention relates to a polypeptide that is modified to have homoserine O-acetyltransferase activity, and in particular, the present invention provides a modified polypeptide having homoserine O-acetyltransferase activity, in which the amino acid at position 111 of a polypeptide having homoserine succinyltransferase activity is substituted with other amino acid.
    Type: Grant
    Filed: December 21, 2011
    Date of Patent: September 8, 2015
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: So Young Kim, Yong Uk Shin, Chang Il Seo, In Kyung Heo, Ju Eun Kim, Hyun Ah Kim, Han Jin Lee, Kwang Ho Na, Sung Kwang Son
  • Patent number: 9040261
    Abstract: A method of preventing or inhibiting L-cystine crystallization using the compounds of formula I is disclosed. wherein A, L, R1a, R1b, and m are as described herein. The compounds may be prepared as pharmaceutical compositions, and may be used for the prevention and treatment of conditions that are causally related to L-cystine crystallization, such as comprising (but not limited to) kidney stones.
    Type: Grant
    Filed: May 24, 2013
    Date of Patent: May 26, 2015
    Assignee: NEW YORK UNIVERSITY
    Inventors: Michael D. Ward, Jeffrey Rimer
  • Publication number: 20150140614
    Abstract: The invention relates to an isolated polynucleotide having promoter activity, a variant of the promoter of the gap gene coding for glyceraldehyde-3-phosphate dehydrogenase; and to a microorganism which produces and/or secretes a fine chemical, the microorganism including the isolated polynucleotide having promoter activity, which enables various genes to be overexpressed in comparison with the particular starting strain; and to a process for preparing fine chemicals using the microorganism.
    Type: Application
    Filed: January 28, 2015
    Publication date: May 21, 2015
    Applicant: EVONIK DEGUSSA GMBH
    Inventors: ALEXANDER RETH, BRIGITTE BATHE, STEPHAN HANS, WILFRIED CLAES
  • Patent number: 9034611
    Abstract: The present invention is related to a microorganism for the production of methionine, wherein said microorganism is modified to enhance the transhydrogenase activity of PntAB. In a preferred aspect of the invention, the activity of the transhydrogenase UdhA is attenuated in said microorganisms. The invention also related to a method for producing methionine by fermentation.
    Type: Grant
    Filed: October 24, 2011
    Date of Patent: May 19, 2015
    Assignee: METALBOLIC EXPLORER
    Inventors: Wanda Dischert, Perrine Vasseur, Cédric Boisart, Rainer Figge
  • Patent number: 9029105
    Abstract: The present invention relates to a method for producing L-methionine, comprising: i) culturing an L-methionine precursor-producing microorganism strain in a fermentation solution, so that the L-methionine precursor accumulates in the solution; and ii) mixing a converting enzyme and methylmercaptan or its salts with at least a portion of the solution to convert the accumulated L-methionine precursor into L-methionine, as well as to microorganism strains used in each step.
    Type: Grant
    Filed: January 29, 2013
    Date of Patent: May 12, 2015
    Assignee: CJ Cheiljedang Corporation
    Inventors: So-young Kim, Kwang-myung Choi, Yong-uk Shin, Hye-won Um, Kyung-oh Choi, Jin-sook Chang, Young-wook Cho, Young-hoon Park
  • Patent number: 9023623
    Abstract: The present invention provides a preparing method of an allicin injection and the low temperature continuous stirring ultrafiltration device thereof. Said preparing method consists of the following steps: extracting allicin; diluting the allicin with solvent precooled to 1-4 in a clean environment, adding nitrogen gas or argon gas, and then encapsulating the solution to obtain allicin injection with different specifications.
    Type: Grant
    Filed: December 21, 2012
    Date of Patent: May 5, 2015
    Assignee: Xinjiang Ailexin Pharmaceutical Co., Ltd.
    Inventors: Jian Chen, Xinxia Li
  • Patent number: 9005952
    Abstract: The present invention relates to a microorganism producing L-methionine precursor, O-acetylhomoserine, and a method of producing L-methionine precursor using the microorganism.
    Type: Grant
    Filed: April 4, 2008
    Date of Patent: April 14, 2015
    Assignee: CJ Cheiljedang Corporation
    Inventors: Chul Ha Kim, So Young Kim, Young Uk Shin, Hye Won Um, Jin Sook Chang, Young Wook Cho, Han Jin Lee, In Kyung Heo, Chang Il Seo, Kwang Ho Na
  • Patent number: 8969048
    Abstract: The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.
    Type: Grant
    Filed: May 2, 2014
    Date of Patent: March 3, 2015
    Assignee: Ajinomoto Co., Inc.
    Inventors: Yury Ivanovich Kozlov, Akito Chinen, Hiroshi Izui, Yoshihiko Hara, Hisashi Yasueda, Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Joanna Yosifovna Katashkina
  • Publication number: 20150056666
    Abstract: The invention relates to an isolated polynucleotide having promoter activity, a variant of the promoter of the gap gene coding for glyceraldehyde-3-phosphate dehydrogenase; and to a microorganism which produces and/or secretes a fine chemical, the microorganism including the isolated polynucleotide having promoter activity, which enables various genes to be overexpressed in comparison with the particular starting strain; and to a process for preparing fine chemicals using the microorganism.
    Type: Application
    Filed: November 4, 2014
    Publication date: February 26, 2015
    Applicant: Evonik Degussa GmbH
    Inventors: Alexander RETH, Brigitte Bathe, Stephan Hans, Wilfried Claes
  • Publication number: 20150056665
    Abstract: A method of producing a chemical includes culturing cells in a culture solution in a fermentor to ferment a feedstock to produce a chemical; supplying the culture solution containing the chemical produced in the culturing to a plurality of separation membrane units arranged in parallel; filtering the culture solution supplied in the supplying to separate a permeate containing the chemical; refluxing a retentate that is not filtered in the filtering to the fermentor; and supplying a gas containing oxygen to the plurality of separation membrane units while a supply amount is changed to at least two different values to perform scrubbing, wherein the supply amount and supply time of the gas containing oxygen supplied in the culturing and the supplying the gas are set so that a kLa value is within a predetermined range from an optimal kLa value for the cells cultured in the culturing.
    Type: Application
    Filed: March 27, 2013
    Publication date: February 26, 2015
    Inventors: Satoko Kanamori, Hideki Sawai, Norihiro Takeuchi
  • Patent number: 8962284
    Abstract: A novel technique for improving the production by bacteria of amino acids that contain sulfur has been developed, and thereby a sulfur-containing amino acid-producing bacterium, and a method for producing a compound such as a sulfur-containing amino acid are provided.
    Type: Grant
    Filed: March 4, 2013
    Date of Patent: February 24, 2015
    Assignee: Ajinomoto Co., Inc.
    Inventor: Gen Nonaka
  • Publication number: 20150004658
    Abstract: Provision of a composition stably comprising a high concentration of sulfur amino acid derived from a plant belonging to the genus Allium. A method for producing a sulfur amino acid-comprising composition comprising: heating a plant belonging to the genus Allium; treating the plant belonging to the genus Allium thus heated with a ?-glutamyl bond cleaving enzyme; and subjecting the resulting enzyme-treated product to ion exchange chromatography.
    Type: Application
    Filed: March 13, 2013
    Publication date: January 1, 2015
    Applicant: NISSHIN PHARMA INC.
    Inventors: Masataka Harata, Yuto Inagawa
  • Publication number: 20150004657
    Abstract: The present invention relates to a method for producing cysteine or derivatives thereof using novel O-phosphoserine sulfhydrylase. According to the present invention, a method for producing cysteine by novel O-phosphoserine sulfhydrylase (OPSS) using O-phosphoserine as a substrate is provided, and this method is advantageous in that cysteine can be simply and environmental-friendly produced in a high yield.
    Type: Application
    Filed: December 14, 2012
    Publication date: January 1, 2015
    Inventors: Byeong Cheol Song, Jin Sook Chang, Jae Hyun Jo, Hye Won Kim
  • Publication number: 20140315261
    Abstract: The present invention provides a method for producing L-amino acids by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to attenuate expression of the yjjK gene.
    Type: Application
    Filed: April 23, 2014
    Publication date: October 23, 2014
    Applicant: AJINOMOTO CO., INC.
    Inventors: Natalia Viktorovna Stoynova, Valery Vasilievich Samsonov, Natalia Sergeevna Eremina, Evgeniya Aleksandrovna Polyakova
  • Patent number: 8852897
    Abstract: A method for producing an L-amino acid is described using a bacterium of the Enterobacteriaceae family, wherein the bacterium contains a protein which is able to confer resistance to growth inhibition by L-cysteine.
    Type: Grant
    Filed: May 3, 2013
    Date of Patent: October 7, 2014
    Assignee: Ajinomoto Co., Inc.
    Inventors: Ekaterina Alekseevna Savrasova, Natalia Viktorovna Stoynova, Gen Nonaka, Shunsuke Yamazaki, Kazuhiro Takumi
  • Publication number: 20140242643
    Abstract: The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.
    Type: Application
    Filed: May 2, 2014
    Publication date: August 28, 2014
    Applicant: AJINOMOTO CO., INC.
    Inventors: Yury Ivanovich Kozlov, Akito Chinen, Hiroshi Izui, Yoshihiko Hara, Hisashi Yasueda, Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Joanna Yosifovna Katashkina
  • Publication number: 20140227746
    Abstract: The present invention relates to a method for producing L-methionine using a bio-synthesis process and a specific enzymatic process. More particularly, the present invention relates to a method for producing L-methionine with high yield by enzyme conversion reaction from L-methionine precursor in the presence of methyl mercaptan (CH3SH). The process of the present invention enables selective production of L-methionine which may be used in various fields of industry, such as feed- and food-additives, as raw material for medical supplies, pharmaceutical drugs, and the like.
    Type: Application
    Filed: September 2, 2011
    Publication date: August 14, 2014
    Applicants: CJ CHEILJEDANG CORPORATION, ARKEMA FRANCE
    Inventors: Georges Fremy, Patrice Barre, So Young Kim, Sung Kwang Son, Sang Mok Lee
  • Patent number: 8802399
    Abstract: A method for the production of natural L-cysteine by fermentation in a production fermenter in which a microorganism strain is cultured in a fermentation medium, characterized in that the fraction of the compounds L-cysteine, L-cystine and thiazolidine in the fermentation medium is controlled in a targeted manner by an iron concentration of a maximum of 8 mg/l in the fermentation medium.
    Type: Grant
    Filed: June 25, 2012
    Date of Patent: August 12, 2014
    Assignee: Wacker Chemie AG
    Inventors: Anneliese Reutter-Maier, Markus Brunner, Tobias Dassler
  • Patent number: 8795990
    Abstract: The present invention relates to the use of recombinant homoserine transsuccinylase enzymes with altered feedback sensitivity (MetA*) and possibly recombinant S-adenosyl methionine synthetase enzymes with reduced activity (MetK*) for the production of methionine, its precursors or derivatives thereof.
    Type: Grant
    Filed: May 12, 2005
    Date of Patent: August 5, 2014
    Assignee: Metabolic Explorer
    Inventors: Gwénaëlle Bestel-Corre, Michel Chateau, Rainer Figge, Céline Raynaud, Philippe Soucaille
  • Publication number: 20140162325
    Abstract: The present invention provides a method for producing L-amino acid using a bacterium belonging to the family Enterobacteriaceae, particularly a motile bacterium belonging to the genus Escherichia, Enterobacter or Pantoea, wherein the bacterium has been modified so that expression of at least one gene of the flagella formation and motility cascade is enhanced.
    Type: Application
    Filed: February 11, 2014
    Publication date: June 12, 2014
    Applicant: AJINOMOTO CO., INC.
    Inventors: Irina Borisovna Altman, Tatyana Abramovna Yampolskaya, Leonid Romanovich Ptitsyn
  • Patent number: 8748136
    Abstract: The present invention relates to a method for the production of methionine or its derivatives by culturing a microorganism in an appropriate culture medium comprising a source of carbon and a source of sulphur. The microorganism and/or the culture medium and/or the process parameters were modified in a way that the accumulation of the by-product N-acyl-methionine (NAM) is reduced. The isolation of methionine or its derivatives from the fermentation medium is also claimed.
    Type: Grant
    Filed: February 12, 2009
    Date of Patent: June 10, 2014
    Assignee: Metabolic Explorer
    Inventors: Rainer Figge, Philippe Soucaille, Gwénaëlle Bestel-Corre
  • Publication number: 20140141474
    Abstract: A method for the production of natural L-cysteine by fermentation in a production fermenter in which a microorganism strain is cultured in a fermentation medium, characterized in that the fraction of the compounds L-cysteine, L-cystine and thiazolidine in the fermentation medium is controlled in a targeted manner by an iron concentration of a maximum of 8 mg/l in the fermentation medium.
    Type: Application
    Filed: June 25, 2012
    Publication date: May 22, 2014
    Applicant: Wacker Chemie AG
    Inventors: Anneliese Reutter-Maier, Markus Brunner, Tobias Dassler
  • Publication number: 20140141473
    Abstract: Yeast cell belonging to the genus Saccharomyces having introduced into its genome at least one xylA gene and at least one of each of araA, araB and araD genes and that is capable of consuming a mixed sugar mixture comprising glucose, xylose and arabinose, wherein the cell co-consumes glucose and arabinose, has genetic variations obtained during adaptive evolution and has a specific xylose consumption rate in the presence of glucose that is 0.25 g xylose/h, g DM or more.
    Type: Application
    Filed: April 20, 2012
    Publication date: May 22, 2014
    Applicant: DSM IP ASSETS B.V.
    Inventors: Paul Klaassen, Bianca Elisabeth Maria Gielesen, Gijsberdina Pieternella Van Suylekom, Panagiotis Sarantinopoulos, Wilbert Herman Marie Heijne, Aldo Greeve
  • Publication number: 20140134680
    Abstract: The present invention is related to a recombinant microorganism for improved methionine production comprising modifications to produce methionine from glucose as main carbon source by fermentation, and modifications to improve glucose import, wherein the glucose import is improved by modifying the expression of at least one gene selected from ptsG, sgrT sgrS and dgsA. The invention is also related to a method for the fermentative production of methionine or methionine derivatives comprising the steps of: culturing the recombinant microorganism as described above in an appropriate culture medium comprising a fermentable source of carbon containing glucose and a source of sulphur, and recovering methionine or methionine derivatives from the culture medium.
    Type: Application
    Filed: June 29, 2012
    Publication date: May 15, 2014
    Inventors: Wanda Dischert, Rainer Figge
  • Patent number: 8703446
    Abstract: The present invention provides a method for producing L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to enhance the expression of the bssR gene, which encodes a regulator of biofilm through signal secretion.
    Type: Grant
    Filed: January 7, 2013
    Date of Patent: April 22, 2014
    Assignee: Ajinomoto Co., Inc.
    Inventors: Mikhail Yurievich Kiryukhin, Mikhail Markovich Gusyatiner
  • Patent number: 8691537
    Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the rcsA gene.
    Type: Grant
    Filed: November 26, 2008
    Date of Patent: April 8, 2014
    Assignee: Ajinomoto Co., Ltd.
    Inventors: Dmitriy Vladimirovich Filippov, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner
  • Publication number: 20140080186
    Abstract: The invention relates to a method for producing L-cystine by fermenting a microorganism strain in a fermentation medium, in which method L-cystine is precipitated in an amount of at least 70% relative to the total cysteine, characterized in that the O2 saturation of the fermentation medium is kept at least at 1% and at most at 40±3% during the formation of L-cystine.
    Type: Application
    Filed: May 4, 2012
    Publication date: March 20, 2014
    Applicant: WACKER CHEMIE AG
    Inventors: Tobias Dassler, Anneliese Reutter-Maier, Thomas Schloesser
  • Patent number: 8673597
    Abstract: A bacterium which belongs to the family Enterobacteriaceae, and has an ability to produce L-lysine, L-threonine, L-asparagine, L-aspartic acid, L-methionine, L-alanine, L-isoleucine, and/or L-homoserine. The bacterium has been modified so that expression of the gltP and/or gltS genes is/are increased when cultured in a medium, resulting in the accumulation of the L-amino acid(s) in the medium or bacterial cells.
    Type: Grant
    Filed: May 24, 2011
    Date of Patent: March 18, 2014
    Assignee: Ajinomoto Co., Inc.
    Inventors: Yuori Wakasa, Ryo Takeshita
  • Patent number: 8647847
    Abstract: An L-cysteine-producing bacterium is provided, as well as a method for producing L-cysteine etc. using the bacterium by developing a novel technique for improving L-cysteine-producing ability of a bacterium. By culturing a bacterium belonging to the family Enterobacteriaceae, which has L-cysteine-producing ability and is modified so that activity of a protein encoded by the yciW gene, for example, a protein defined by the following (A) or (B), is reduced, in a medium, and collecting L-cysteine, L-cystine, a derivative thereof, or a mixture thereof from the medium, these compounds are produced: (A) a protein having the amino acid sequence shown in SEQ ID NO: 2, (B) a protein having the amino acid sequence shown in SEQ ID NO: 2, but which includes substitution, deletion, insertion, or addition of one or several amino acid residues, reduction of which activity in the bacterium results in improvement in the L-cysteine-producing ability.
    Type: Grant
    Filed: May 29, 2012
    Date of Patent: February 11, 2014
    Assignee: Ajinomoto Co., Inc.
    Inventor: Gen Nonaka
  • Publication number: 20130330787
    Abstract: A method produces a chemical through continuous fermentation including: (a) culturing a cell in a culture medium in a fermentor to ferment a feedstock to produce a chemical; (b) conducting filtration of the culture medium with a separation membrane module; (c) separating a permeate containing the chemical from the culture medium while retaining a non-permeated liquid in the fermentor, and (d) supplying a gas from at least one of a lower portion of the separation membrane module and a pipe communicating between the fermentor and the separation membrane module to adjust a gas linear velocity in the separation membrane module to 0.15 cm/s to 70 cm/s while supplying the separation membrane module with a liquid.
    Type: Application
    Filed: December 8, 2011
    Publication date: December 12, 2013
    Inventors: Satoko Kanamori, Jihoon Cheon, Takashi Mimitsuka, Norihiro Takeuchi, Makoto Nishida, Yuji Tanaka
  • Publication number: 20130323784
    Abstract: A target substance can be efficiently produced by culturing, in a medium, a coryneform bacterium in which the activity of a PTS protein relating to fructose uptake is reduced or lost as compared with a parent strain and the bacterium can produce the target substance, allowing the target substance to form and accumulate in a culture; and collecting the target substance from the culture
    Type: Application
    Filed: February 9, 2012
    Publication date: December 5, 2013
    Applicant: KYOWA HAKKO BIO CO., LTD.
    Inventors: Tetsuro Ujihara, Tetsuya Abe, Makoto Yagasaki
  • Publication number: 20130288313
    Abstract: An L-amino acid is produced by culturing a microorganism belonging to the family Enterobacteriaceae having an L-amino acid-producing ability and modified so that glycerol dehydrogenase and dihydroxyacetone kinase activities are increased, in a medium containing glycerol as a carbon source to produce and accumulate an L-amino acid in the medium or cells, and collecting the L-amino acid from the medium or the cells.
    Type: Application
    Filed: July 10, 2013
    Publication date: October 31, 2013
    Inventors: Yuri Nagai, Kazuyuki Hayashi, Takuji Ueda, Yoshihiro Usuda, Kazuhiko Matsui
  • Publication number: 20130273601
    Abstract: The present invention relates to a yeast cell comprising one or more exogenous genes of a pentose metabolic pathway non-native to the yeast cell wherein the yeast cell has a disruption of the hxk1, hxk2 glk1 and gal1 native in the yeast cell. The invention further relates to pentose and glucose fermenting yeast cell that is capable of simultaneous pentose and glucose consumption.
    Type: Application
    Filed: October 11, 2011
    Publication date: October 17, 2013
    Applicant: DSM IP ASSETS B.V.
    Inventors: Hendrik Wouter Wisselink, Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk
  • Publication number: 20130273614
    Abstract: The present invention relates to a novel protein having O-acetylhomoserine sulfhydrylase activity, a mutant protein thereof, a polynucleotide encoding the same, a recombinant vector comprising the polynucleotide, a microorganism transformed with the recombinant vector, and a method for producing methionine or acetic acid using the protein. The production method of the present invention has the advantage of producing L-methionine and acetic acid cost-effectively through having higher conversion rate and reduced reaction time compared to the existing methods, and it can minimize the amount of enzyme homogenate added when using the mutant protein, thereby easily producing L-methionine and acetic acid at high yield.
    Type: Application
    Filed: December 21, 2011
    Publication date: October 17, 2013
    Applicant: CJ CHEILJEDANG CORPORATION
    Inventors: So Young Kim, Yong Uk Shin, Chang Il Seo, Sung Kwang Son, In Kyung Heo, Han Jin Lee, Ju Eun Kim, Hyun Ah Kim, Jee Yeon Bae, Kwang Ho Na
  • Patent number: 8557549
    Abstract: The present invention provides methods for the production of cysteine or derivates thereof by culturing a microorganism having reduced activity of endogenous phosphoserine phosphatase and enhanced activity of phosphoglycerate dehydrogenase and/or phosphoserine aminotransferase. The O-phosphoserine produced by such an organism can then be reacted with a sulfide in the presence of a sulfydrylase or a microorganism expressing a sulfhydrylase to produce cysteine or a derivative thereof. Microorganisms having the properties noted above are also provided herein.
    Type: Grant
    Filed: October 20, 2011
    Date of Patent: October 15, 2013
    Assignee: CJ Cheildjedang Corporation
    Inventors: Jin Sook Chang, Jae Hyun Jo, Hyun Ae Bae, Byeong Cheol Song, Sol Kim, Hye Won Kim
  • Patent number: 8551741
    Abstract: An L-amino acid is produced by culturing a bacterium of the Enterobacteriaceae family which has an L-amino acid-producing ability in a medium containing fatty acids as the carbon source, particularly fatty acids which have been subjected to emulsification or homogenization, to thereby produce and accumulate the L-amino acid in a culture medium; and collecting the L-amino acid from the culture medium.
    Type: Grant
    Filed: June 5, 2009
    Date of Patent: October 8, 2013
    Assignee: Ajinomoto Co., Inc.
    Inventors: Yoshihiro Usuda, Seizaburo Shiraga, Kazuhiko Matsui, Shigeo Suzuki
  • Patent number: 8551742
    Abstract: Described herein are microorganisms that produce methionine and related products from endogenous genes in a transsulfuration pathway, as well as from exogenous genes providing a direct sulfhydrylation pathway. Novel genes that are useful for methionine and SAMe production are disclosed.
    Type: Grant
    Filed: April 11, 2007
    Date of Patent: October 8, 2013
    Assignee: CJ Cheiljedang Corporation
    Inventors: Brian Brazeau, Jin-Sook Chang, Kwang Myung Cho, Young Wook Cho, Mervyn Desouza, Holly J. Jessen, So-Young Kim, Wei Niu, Fernando A. Sanchez-Riera, Yong-Uk Shin, Hyewon Um
  • Publication number: 20130260424
    Abstract: Described herein are microorganisms that produce methionine and related products from endogenous genes in a transsulfuration pathway, as well as from exogenous genes providing a direct sulfhydrylation pathway. Novel genes that are useful for methionine and SAMe production are disclosed.
    Type: Application
    Filed: March 15, 2013
    Publication date: October 3, 2013
    Applicant: CJ Cheiljedang Corporation
    Inventors: Brian BRAZEAU, Jin-Sook Chang, Kwang Myung Cho, Young Wook Cho, Mervyn Desouza, Holly J. Jessen, So-Young Kim, Wei Niu, Fernando A. Sanchez-Reira, Yong-Uk Shin, Hyewon Um
  • Publication number: 20130261331
    Abstract: The present invention provides a simple industrial process for producing an L- or D-optically active ?-methylcysteine derivative or its salt, which is a useful pharmaceutical intermediate, from readily available, inexpensive raw materials. In a process for producing an L- or D-optically active ?-methylcysteine derivative or its salt, a racemic N-carbamoyl-?-methylcysteine derivative or its salt is D-selectively cyclized with hydantoinase to produce a D-5-methyl-5-thiomethylhydantoin derivative or its salt and an N-carbamoyl-?-methyl-L-cysteine derivative or its salt, which are then subjected to deprotection of the amino group and the sulfur atom, and hydrolysis.
    Type: Application
    Filed: May 17, 2013
    Publication date: October 3, 2013
    Inventors: Takahiro Ohishi, Hirokazu Nanba, Masanobu Sugawara, Masashi Izumida, Tatsuya Honda, Kohei Mori, Satohiro Yanagisawa, Nabuo Nagashima, Kenji Inoue
  • Publication number: 20130231503
    Abstract: The present invention relates to a method for producing L-methionine, comprising: i) culturing an L-methionine precursor-producing microorganism strain in a fermentation solution, so that the L-methionine precursor accumulates in the solution; and ii) mixing a converting enzyme and methylmercaptan or its salts with at least a portion of the solution to convert the accumulated L-methionine precursor into L-methionine, as well as to microorganism strains used in each step.
    Type: Application
    Filed: January 29, 2013
    Publication date: September 5, 2013
    Applicant: CJ CHEILJEDANG CORPORATION
    Inventors: So-young Kim, Kwang-myung Choi, Yong-uk Shin, Hye-won Um, Kyung-oh Choi, Jin-sook Chang, Young-wook Cho, Young-hoon Park