Methionine; Cysteine; Cystine Patents (Class 435/113)
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Publication number: 20090226982Abstract: L-Cysteine is produced by culturing a microorganism having an ability to produce L-cysteine and modified so that expression of emrAB, emrKY, yojIH, acrEF, bcr, or cusA gene should be enhanced in a medium to produce and accumulate L-cysteine in the medium and collecting the L-cysteine from the medium. Genes coding for novel L-cysteine-excreting proteins are identified, and utilized for breeding of L-cysteine-producing microorganism to provide a novel method of producing L-cysteine.Type: ApplicationFiled: July 11, 2007Publication date: September 10, 2009Applicant: AJINOMOTO CO., INCInventors: Hiroshi TAKAGI, Shigeru Nakamori, Akihito Yamaguchi, Kunihiko Nishino
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Publication number: 20090221027Abstract: The present invention pertains to improved microorganisms and methods for the production of methionine and other sulfur containing fine chemicals using the metI gene from Bacillus subtilis or a gene related to metI. In some embodiments of the present invention, the metI gene or another gene is integrated in a fashion that allows for co-production of a water soluble compound such as methionine or other amino acid and a caortenoid compound.Type: ApplicationFiled: July 18, 2006Publication date: September 3, 2009Applicant: BASF AGInventors: Oskar Zelder, Andrea Herold, Corinna Klopprogge, Hartwig Schröder, R. Rogers Yocum, Mark Williams
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Publication number: 20090215129Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to attenuate expression of the leuO gene.Type: ApplicationFiled: September 4, 2007Publication date: August 27, 2009Inventors: Konstantin Vyacheslavovich Rybak, Elvira Borisovna Voroshilova, Tatyana Viktorovna Leonova, Yury Ivanovich Kozlov
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Publication number: 20090215130Abstract: An L-amino acid is produced by culturing an L-amino acid-producing bacterium which belongs to the Enterobacteriaceae family and which has been modified so that the activity of an iron transporter is increased by enhancing expression of one or more genes of the following genes: tonB gene, fepA gene, and fecA.Type: ApplicationFiled: July 25, 2008Publication date: August 27, 2009Inventors: Mayu Iyo, Ryo Takeshita, Shinichi Sugimoto
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Publication number: 20090209011Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to enhance expression of at least one gene of the fucPIKUR operon.Type: ApplicationFiled: December 7, 2007Publication date: August 20, 2009Inventors: Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Marina Evgenievna Sheremet'eva, Aleksandra Yurievna Skorokhodova, Vitaly Grigorievich Paraskevov
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Patent number: 7575905Abstract: The invention relates to a process for the preparation of L-amino acids by the fermentation of recombinant microorganisms of the family Enterobacteriaceae. The microorganisms produce the desired L-amino acid and overexpress the E. coli or S. typhimurium yibD gene encoding the putative glycosyl transferase enzyme. The microorganisms are cultivated in a medium under conditions in which the desired L-amino acid is enriched in the medium or in the cells. The amino acid is then isolated, optionally with constituents of the fermentation broth, and/or all or part (?0 to 100%) of the biomass.Type: GrantFiled: March 8, 2004Date of Patent: August 18, 2009Assignee: Evonik Degussa GmbHInventors: Mechthild Rieping, Nicole Dusch
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Patent number: 7563602Abstract: The invention relates to mutants and alleles of the gnd gene from coryneform bacteria coding for 6-phosphogluconate dehydrogenases which contain at position 329 or a comparable position of the amino acid sequence any amino acid other than L-valine, and to processes for the production of amino acids, preferably L-lysine and L-tryptophan, by fermentation using bacteria that contain these alleles.Type: GrantFiled: January 18, 2008Date of Patent: July 21, 2009Assignee: Degussa AG, Intellectural Property ManagementInventors: Georg Thierbach, Brigitte Bathe, Natalie Schischka
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Publication number: 20090170170Abstract: The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.Type: ApplicationFiled: July 16, 2005Publication date: July 2, 2009Applicant: BASF AktiengesellschaftInventors: Jong-Soo Choi, Weol Kyu Jeong, Il Kwon Kim, Seong Han Lim, Heung-Shick Lee
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Publication number: 20090170169Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the rspAB operon.Type: ApplicationFiled: December 30, 2008Publication date: July 2, 2009Inventors: Dmitriy Vladimirovich Filippov, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner
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Publication number: 20090137011Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the rcsA gene.Type: ApplicationFiled: November 26, 2008Publication date: May 28, 2009Inventors: Dmitriy Vladimirovich Filippov, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner
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Patent number: 7534590Abstract: A solution to be treated containing monatin and impurities is treated with a nonpolar resin having aromatic rings, whereby monatin is separated from the solution. Treatment with the nonpolar resin having aromatic rings results in efficient separation of monatin under a mild pH condition.Type: GrantFiled: December 27, 2005Date of Patent: May 19, 2009Assignee: AJINOMOTO Co., Inc.Inventors: Ken-ichi Mori, Masakazu Sugiyama, Tadashi Takemoto
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Publication number: 20090104667Abstract: An L-amino acid is produced by culturing a microorganism which belongs to the family Enterobacteriaceae and is able to produce an L-amino acid, wherein the bacterium has been modified to enhance orotate phosphoribosyltransferase activity is enhanced, in a medium to produce and cause accumulation of an L-amino acid in the medium or cells, and collecting the L-amino acid from the medium or the cells.Type: ApplicationFiled: October 27, 2008Publication date: April 23, 2009Inventors: Yoko Asakura, Yuri Nagai, Takuji Ueda
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Publication number: 20090098621Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of a gene coding for sRNA.Type: ApplicationFiled: September 18, 2008Publication date: April 16, 2009Inventors: Konstantin Vyacheslavovich Rybak, Aleksandra Yurievna Skorokhodova, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner, Tatyana Viktorovna Leonova, Yury Ivanovich Kozlov, Takuji Ueda, Vitaly Grigorievich Paraskevov
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Publication number: 20090087886Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the sfmACDFH-fimZ cluster and/or the fimZ gene.Type: ApplicationFiled: October 17, 2008Publication date: April 2, 2009Inventors: Dmitriy Vladimirovich Filippov, Vera Georgievna Doroshenko, Aleksandra Yurievna Skorokhodova, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner
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Publication number: 20090081738Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to attenuate expression of the bolA gene.Type: ApplicationFiled: July 31, 2007Publication date: March 26, 2009Inventors: Dmitriy Vladimirovich Filippov, Elvira Borisovna Voroshilova, Tatyana Viktorovna Leonova, Yuri Ivanovich Kozlov, Vitaly Grigorievich Paraskevov
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Publication number: 20090068711Abstract: The invention relates to a method for the production of L-amino acids by fermentation of recombinant microorganisms of the Enterobacteriaceae family, characterized in that a) the microorganisms producing the desired L-amino acid and wherein the lamB-gene or nucleotide sequence coding for the gene product maltoporin is amplified, particularly overexpressed, are cultivated in a medium in conditions enabling the desired L-amino acid to be enriched in the medium or in cells, and b) the desired L-amino acid is isolated, wherein constituents of the fermentation broth and/or biomass remain in the entirety thereof or in parts thereof (=0 bis 100%) in the isolated product or are fully removed.Type: ApplicationFiled: July 5, 2005Publication date: March 12, 2009Applicant: Degussa GmbHInventor: Mechthild Rieping
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Publication number: 20090053778Abstract: The present invention relates to microorganisms and methods for producing at least one sulfur-containing compound.Type: ApplicationFiled: July 20, 2005Publication date: February 26, 2009Applicant: FRANCE TELECOMInventors: Uwe Sauer, Jorg Mampel, Oskar Zelder, Andrea Herold, Corinna Klopprogge, Hartwig Schroder, Stefan Haefner
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Patent number: 7485444Abstract: The invention relates to methods for the fermentative production of sulfur-containing fine chemicals, in particular L-methionine, by using bacteria which express a nucleotide sequence coding for a methionine synthase (metH) gene.Type: GrantFiled: April 16, 2003Date of Patent: February 3, 2009Assignee: Evonik Degussa GmbHInventors: Burkhard Kröger, Oskar Zelder, Corinna Klopprogge, Hartwig Schröder, Stefan Häfner
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Publication number: 20090029424Abstract: The present invention relates to the use of recombinant homoserine transsuccinylase enzymes with altered feedback sensitivity (MetA*) and possibly recombinant S-adenosyl methionine synthetase enzymes with reduced activity (MetK*) for the production of methionine, its precursors or derivatives thereof.Type: ApplicationFiled: May 12, 2005Publication date: January 29, 2009Applicant: Metabolic ExplorerInventors: Gwenaelle Bestel-Corre, Michel Chateau, Rainer Figge, Celine Raynaud, Philippe Soucaille
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Patent number: 7476531Abstract: There is provided a method for producing L-threonine, L-valine, L-proline, L-leucine, L-methionine and L-arginine using bacterium belonging to the genus Escherichia wherein L-amino acid productivity of the bacterium is enhanced by enhancing an activity of proteins coded by b2682 and b2683 genes, or protein coded by b1242 or b3434 gene.Type: GrantFiled: February 13, 2002Date of Patent: January 13, 2009Assignee: Ajinomoto Co., Inc.Inventors: Ekaterina Aleksandrovna Tabolina, Konstantin Vyacheslavovich Rybak, Evgeni Moiseevich Khourges, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner
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Publication number: 20090004705Abstract: The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.Type: ApplicationFiled: February 13, 2008Publication date: January 1, 2009Applicant: BASF AktiengesellschaftInventors: Burkhard Kroger, Oskar Zelder, Corinna Klopprogge, Hartwing Schroder, Stefan Haefner
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Patent number: 7470525Abstract: There is provided a process for producing an optically active 2-alkylcysteine or a salt thereof, characterized by allowing cells of microorganism or treated products thereof having an activity of stereoselective hydrolysis of the amide bond of a 2-alkyl-L-cysteinamide or a salt thereof to act on a 2-alkylcysteinamide consisting of a mixture of D- and L-isomers or a salt thereof; and allowing the obtained 2-alkyl-L-cysteine and 2-alkyl-D-cysteinamide to react with an aldehyde or a ketone, or an acetal or ketal thereof, so as to derive therefrom a 4-alkylthiazolidine-4-carboxylic acid or a salt thereof and a 4-alkylthiazolidine-4-carboxamide or a salt thereof, thereby efficiently separating and obtaining a 2-alkyl-L-cysteine or a salt thereof, or a 2-alkyl-D-cysteine or a salt thereof. There is also provided a process for producing a 4-alkylthiazolidine-4-carboxylic acid or a salt thereof from the 2-alkylcysteine or a salt thereof.Type: GrantFiled: November 18, 2004Date of Patent: December 30, 2008Assignee: Mitsubishi Gas Chemical Company, Inc.Inventors: Yasushi Higuchi, Akinori Tanaka, Ryuji Hasemi, Masaki Sugita
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Publication number: 20080318790Abstract: The present invention relates to a process for the production of methionine in an organism such as a microorganism, a non-human animal or plant. The invention furthermore relates to nucleic acid molecules, polypeptides, nucleic acid constructs, vectors, antisense molecules, antibodies, host cells, plant tissue, propagation material, harvested material, plants, microorganisms as well as agricultural compositions and to their use.Type: ApplicationFiled: March 30, 2005Publication date: December 25, 2008Applicant: Metanomics GmbHInventors: Marcus Ebneth, Piotr Puzio, Astrid Blau, Tilmann B. Walk, Volker Haake, Klaus Däschner, Gunnar Plesch
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Publication number: 20080311632Abstract: A method of producing methionine, derivatives or precursors thereof comprising culturing a microorganism modified to enhance production of cysteine in a culture medium comprising a source of carbon and a source of sulfur and recovering methionine from the culture medium. A microorganism for fermentative production of methionine or its derivatives in which production of methionine or derivatives thereof, wherein the microorganism enhances production of cysteine.Type: ApplicationFiled: January 4, 2006Publication date: December 18, 2008Inventors: Rainer Figge, Fabien Lux, Celine Raynaud, Michel Chateau, Philippe Soucaille
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Publication number: 20080293109Abstract: The present invention relates to methods for converting plant cell wall polysaccharides into one or more products, comprising: treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into the one or more products.Type: ApplicationFiled: July 14, 2008Publication date: November 27, 2008Applicant: Novozymes, Inc.Inventors: Randy Berka, Joel Cherry
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Publication number: 20080286841Abstract: The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.Type: ApplicationFiled: February 27, 2008Publication date: November 20, 2008Applicant: BASF AktiengesellschaftInventors: Burkhard Kroger, Oskar Zelder, Corinna Klopprogge, Hartwig Schroder, Stefan Haefner
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Publication number: 20080274516Abstract: The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.Type: ApplicationFiled: December 16, 2004Publication date: November 6, 2008Inventors: Burkhard Kroger, Oskar Zelder, Corinna Klopprogge, Hartwig Schroder, Stefan Haefner
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Patent number: 7435420Abstract: The present invention discloses a method for therapeutically treating an animal, including a human, for psychosomatic, depressive and neuropsychiatric diseases, such as anxiety, depression, insomnia, schizophrenia, epilepsy, spasm and chronic pain. Administration of a suitable DP IV inhibitor causes the reduction of activity in the enzyme dipeptidyl peptidase (DP IV or CD 26) or of DP IV—like enzyme activity in the brain of mammals and leads as a causal consequence to a reduced degradation of the neuropeptide Y (NPY) and similar substrates by DP IV and DP IV-like enzymes. Such treatment will result in a reduction or delay in the decrease of the concentration of functionally active neuronal NPY (1-36).Type: GrantFiled: April 4, 2006Date of Patent: October 14, 2008Assignee: Probiodrug AGInventors: Stephan von Hoersten, Ants Kask, Hans-Ulrich Demuth, Huu Phuc Nguyen, Susanne Kruber, Matthias Hoffmann
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Patent number: 7432085Abstract: In a method for producing an L-amino acid by culturing a microorganism having an ability to produce an L-amino acid in a medium to produce and accumulate the L-amino acid in the medium and collecting the L-amino acid from the medium, a Gram-negative bacterium having the Entner-Doudoroff pathway and modified so that 6-phosphogluconate dehydratase activity or 2-keto-3-deoxy-6-phosphogluconate aldolase activity, or activities of the both are enhanced is used as the microorganism.Type: GrantFiled: November 18, 2005Date of Patent: October 7, 2008Assignee: Ajinomoto Co., Inc.Inventors: Yoshihiko Hara, Hiroshi Izui, Takahiro Asano, Yasuyuki Watanabe, Tsuyoshi Nakamatsu
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Publication number: 20080194030Abstract: Disclosed herein are a method of preparing an L-methionine production strain by overexpressing proteins involved in L-methionine biosynthesis in an L-threonine production strain, a strain prepared by the method, and a method of producing L-methionine using the strain.Type: ApplicationFiled: June 16, 2005Publication date: August 14, 2008Inventors: Young Hoon Park, Kwang Myung Cho, Yong-Uk Shin
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Patent number: 7410789Abstract: A microorganism strain secreting S-adenosylmethionine having increased activity of the cmr (mdfA) gene product is provided.Type: GrantFiled: March 2, 2006Date of Patent: August 12, 2008Assignee: Wacker Chemie AGInventors: Thomas Schlösser, Susanne Leonhartsberger, Renate Flinspach
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Publication number: 20080176295Abstract: The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.Type: ApplicationFiled: March 17, 2008Publication date: July 24, 2008Inventors: Oskar Zelder, Corinna Klopprogge, Burkhard Kroger, Hartwig Schroder, Stefan Haefner
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Publication number: 20080166775Abstract: The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.Type: ApplicationFiled: February 27, 2008Publication date: July 10, 2008Applicant: BASF AktiengesellschaftInventors: Burkhard Kroger, Oskar Zelder, Corinna Klopprogge, Hartwig Schroder, Stefan Haefner
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Publication number: 20080166774Abstract: The invention relates to a method for producing an amino acid comprising culturing a microorganism of the genus Corynebacterium or Brevibacterium wherein said microorganism is partially or completely deficient in at least one of the gene loci of the group which is formed by otsAB, treZ and treS, and subsequent isolation of the amino acid from the culture medium.Type: ApplicationFiled: January 28, 2008Publication date: July 10, 2008Applicant: BASF AGInventors: Corinna Klopprogge, Oskar Zelder, Burkhard Kroger, Hartwig Schroder, Stefan Hafner, Wolfgang Liebl
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Patent number: 7381549Abstract: The invention relates to methods for the fermentative production of sulfur-containing fine chemicals, in particular L-methionine, by using bacteria which express a nucleotide sequence coding for a methionine synthase (metH) gene.Type: GrantFiled: August 26, 2003Date of Patent: June 3, 2008Assignee: BASF AktiengesellschaftInventors: Burkhard Kröger, Oskar Zelder, Corinna Klopprogge, Hartwig Schröder, Stefan Häfner
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Publication number: 20080118959Abstract: The invention relates to methods for the production of sulfur-containing fine chemicals, in particular L-methionine, by fermentation using bacteria in which a nucleotide sequence encoding an S-adenosylmethionine synthase (metK) gene is expressed.Type: ApplicationFiled: May 23, 2003Publication date: May 22, 2008Inventors: Hartwig Schroder, Burkhard Kroger, Oskar Zelder, Corinna Klopprogge, Stefan Hafner
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Publication number: 20080076163Abstract: L-cysteine is produced by culturing an Escherichia bacterium having L-cysteine producing ability and containing a gene encoding an O-acetylserine sulphydrylase B or MalY regulatory protein that is modified so that cysteine desulfhydrase activity is reduced or eliminated. The bacterium is cultured in a medium to produce and cause accumulation of L-cysteine in the medium, and collecting L-cysteine from the medium.Type: ApplicationFiled: March 3, 2005Publication date: March 27, 2008Inventors: Hiroshi Takagi, Shigeru Nakamori, Masaru Wada, Hirotada Mori
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Patent number: 7338790Abstract: The invention relates to mutants and alleles of the gnd gene from coryneform bacteria coding for 6-phosphogluconate dehydrogenases which contain at position 329 or a comparable position of the amino acid sequence any amino acid other than L-valine, and to processes for the production of amino acids, preferably L-lysine and L-tryptophan, by fermentation using bacteria that contain these alleles.Type: GrantFiled: September 16, 2005Date of Patent: March 4, 2008Assignee: Degussa AG, Intellectual Property ManagementInventors: Georg Thierbach, Brigitte Bathe, Natalie Schischka
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Patent number: 7312058Abstract: O-acetylserine, L-cysteine and sulphurous compounds derived therefrom may be produced using a bacterium belonging to the genus Escherichia which harbors a mutant feedback-resistant serine acetyltransferases in which the amino acid sequence corresponding to positions from 89 to 96 in a wild-type serine acetyltransferase is replaced with any one of the amino acid sequences shown in SEQ ID NOS: 4 to 9, and feedback inhibition by L-cysteine in the bacterium is desensitized.Type: GrantFiled: July 16, 2004Date of Patent: December 25, 2007Assignee: Ajinomoto Co., Ltd.Inventors: Tatsuki Kashiwagi, Yuko Kai, Kohki Ishikawa, Ei-ichiro Suzuki, Hiroshi Takagi, Mikhail Kharisovich Ziyatdinov, Ekaterina Igorevna Redkina, Mikhail Markovich Gusyatiner
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Patent number: 7311836Abstract: According to the process for producing amino acid or salt thereof in the present invention, in the adsorption step, an amino acid-containing aqueous solution is fed into a pressure tight column so that a free amino acid is adsorbed on a carbonate-type anion exchange resin packed in the pressure tight column. Subsequently, in the elution step, eluent liquid containing a hydrogen carbonate ion and/or a carbonate ion is injected into the pressure tight column in a pressurized state to elute the amino acid adsorbed on the anion exchange resin and simultaneously to regenarate the anion exchange resin into the carbonate-type. In the case of purifying an acidic amino acid, an aqueous ammonium carbonate solution is employed as the eluent liquid. In the case of purifying a neutral amino acid, an aqueous carbonic acid solution, an aqueous hydrogen carbonate solution, an aqueous ammonium hydrogen carbonate solution or an aqueous ammonium carbonate solution is employed as the eluent liquid.Type: GrantFiled: June 27, 2005Date of Patent: December 25, 2007Assignee: Ajinomoto Co., Inc.Inventors: Yasuhiro Kusunose, Kenji Takahashi
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Patent number: 7300777Abstract: The present invention relates to a mutated pyruvate carboxylase gene from Corynebacterium. The mutant pyruvate carboxylase gene encodes a pyruvate carboxylase enzyme which is resistant to feedback inhibition from aspartic acid. The present invention also relates to a method of replacing the wild-type pyruvate carboxylase gene in Corynebacterium with this feedback-resistant pyruvate carboxylase gene. The present invention further relates to methods of the production of amino acids, preferably lysine, comprising the use of this mutant pyruvate carboxylase enzyme in microorganisms.Type: GrantFiled: May 25, 2005Date of Patent: November 27, 2007Assignees: Archer-Daniels-Midland Company, Massachusetts Institute of TechnologyInventors: Paul D. Hanke, Anthony J. Sinskey, Laura B. Willis, Stephane Guillouet
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Patent number: 7282357Abstract: The invention relates to methods for the fermentative production of sulfur-containing fine chemicals, in particular L-methionine, by using bacteria which express a nucleotide sequence coding for a methionine synthase (metF) gene.Type: GrantFiled: August 26, 2003Date of Patent: October 16, 2007Assignee: BASF AktiengesellschaftInventors: Burkhard Kröger, Oskar Zelder, Corinna Kolpprogge, Hartwig Schröder, Stefan Häfner
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Patent number: 7273920Abstract: The invention relates to novel compounds comprising a ubiquitination recognition element and a protein binding element. The invention also relates to the use of said compounds for modulating the level and/or activity of a target protein. The compounds are useful for the treatment of disease such as infections, inflammatory conditions, cancer and genetic diseases. The compounds are also useful as insecticides and herbicides.Type: GrantFiled: January 16, 2003Date of Patent: September 25, 2007Assignee: Wellstat BioCatalysis, LLCInventors: John H. Kenten, Steven F. Roberts
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Patent number: 7238502Abstract: The invention relates to methods for the fermentative production of sulfur-containing fine chemicals, in particular L-methionine, by using bacteria which express a nucleotide sequence coding for a methionine synthase (metA) gene.Type: GrantFiled: August 26, 2003Date of Patent: July 3, 2007Assignee: BASF AktiengesellschaftInventors: Burkhard Kröger, Oskar Zelder, Corinna Kolpprogge, Hartwig Schröder, Stefan Häfner
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Patent number: 7211415Abstract: The present invention relates to a process for the production of L-amino acids by fermentation of recombinant microorganisms of the Enterobacteriaceae family, wherein a) the yfiD ORF and/or the pflB gene or nucleotide sequences coding for the gene products are overexpressed in the microorganisms producing the desired L-amino acid, and the microorganisms are cultured in a medium under conditions in which the desired L-amino acid is enriched in the medium or in the cells; and b) the desired L-amino acid is isolated, in a manner such that constituents of the fermentation broth and/or the biomass in its entirety or in portions (>0 to 100%) either remain in the isolated product or are completely removed.Type: GrantFiled: April 5, 2004Date of Patent: May 1, 2007Assignee: Degussa AGInventors: Mechthild Rieping, Mike Farwick
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Patent number: 7208631Abstract: A process for producing a 2-alkylcysteinamide, which comprises hydrolysis of a 4-alkylthiazolidine-4-carboxamide represented by the general formula (2) or a salt thereof: wherein R represents a lower alkyl group having 1–4 carbon atoms; and each of R1 and R2 independently represents hydrogen or a lower alkyl group having 1–4 carbon atoms, or R1 and R2 are linked together to form an alicyclic, structure having 4–7 carbon atoms, excluding the case where both R1 and R2 are hydrogen, to give a 2-alkylcysteinamide represented by the general formula (1) or a salt thereof wherein R represents a lower alkyl group having 1–4 carbon atoms. Cells of a microorganism or treated products thereof having activity of stereoselective hydrolysis of a 2-alkyl-L-cysteinamide are allowed to act on the compound represented by the general formula (1) to yield a 2-alkyl-L-cysteine.Type: GrantFiled: April 7, 2004Date of Patent: April 24, 2007Assignee: Mitsubishi Gas Chemical Company, Inc.Inventors: Yasushi Higuchi, Akinori Tanaka, Ryuji Hasemi
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Patent number: 7195897Abstract: The invention relates to a homoserine transsuccinylase, which exhibits reduced sensitivity towards L-methionine or SAM in comparison with a homoserine transsuccinylase wild-type enzyme, whereby the latter comprises an amino acid sequence containing a TyrGlnXaaThrPro sub-sequence, the Thr of said sub-sequence lying between positions 285 and 310 of the amino acid sequence and position 1 being filled by the starter methionine. The inventive homoserine transsuccinylase is characterized in that in comparison with the wild-type enzyme at least 2 amino acids are modified, said modification taking place in the Thr of the sub-sequence or in the C-terminal.Type: GrantFiled: October 16, 2003Date of Patent: March 27, 2007Assignee: Consortium für elektrochemische Industrie GmbHInventors: Susanne Leonhartsberger, Christoph Winterhalter, Kerstin Pfeiffer, Brigitte Bauer
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Patent number: 7192747Abstract: A methane-utilizing microorganism capable of producing L-amino acid, for example, bacteria belonging to type I, type X or type II in the taxonomic categorization methane-utilizing bacteria such as Methylomonas albus, Methylococcus capsulatus and Methylosinus trichosporium, is cultivated in a culture medium in contact with gas containing methane which is the main source of carbon, to allow the L-amino acid to be produced and accumulated in the medium, and the L-amino acid is collected from the medium.Type: GrantFiled: December 4, 2002Date of Patent: March 20, 2007Assignee: Ajinomoto, Co. Inc.Inventors: Yukiko Ono, Hisashi Yasueda, Yoshio Kawahara, Shinichi Sugimoto
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Patent number: 7166436Abstract: The present invention relates to a method of simultaneously identifying and determining the levels of expression of cysteine-containing proteins in normal and perturbed cells, a method for proteomic analysis, a process for preparing fusion proteins, and compounds and reagents related thereto.Type: GrantFiled: August 1, 2002Date of Patent: January 23, 2007Assignee: Syngenta Participations, AGInventors: Paul Haynes, Jing Wei, John Yates, Nancy Andon
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Patent number: 7160570Abstract: The present invention relates to a method for the manufacture of a food material containing high quantities of cysteine. In this method, a yeast extract is prepared from yeast cells that contains ?-glutamylcysteine and the extract is concentrated by controlling the temperature to be not greater than 60° C. to prepare a food material in a liquid form, wherein the concentration of solids is at least 10% and the resulting food material is maintained at 70 to 130° C.Type: GrantFiled: March 10, 2004Date of Patent: January 9, 2007Assignee: Ajinomoto Co., Inc.Inventors: Hiroaki Nishiuchi, Yuki Nishimori, Yasushi Nishimura