Methionine; Cysteine; Cystine Patents (Class 435/113)
  • Publication number: 20090209011
    Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to enhance expression of at least one gene of the fucPIKUR operon.
    Type: Application
    Filed: December 7, 2007
    Publication date: August 20, 2009
    Inventors: Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Marina Evgenievna Sheremet'eva, Aleksandra Yurievna Skorokhodova, Vitaly Grigorievich Paraskevov
  • Patent number: 7575905
    Abstract: The invention relates to a process for the preparation of L-amino acids by the fermentation of recombinant microorganisms of the family Enterobacteriaceae. The microorganisms produce the desired L-amino acid and overexpress the E. coli or S. typhimurium yibD gene encoding the putative glycosyl transferase enzyme. The microorganisms are cultivated in a medium under conditions in which the desired L-amino acid is enriched in the medium or in the cells. The amino acid is then isolated, optionally with constituents of the fermentation broth, and/or all or part (?0 to 100%) of the biomass.
    Type: Grant
    Filed: March 8, 2004
    Date of Patent: August 18, 2009
    Assignee: Evonik Degussa GmbH
    Inventors: Mechthild Rieping, Nicole Dusch
  • Patent number: 7563602
    Abstract: The invention relates to mutants and alleles of the gnd gene from coryneform bacteria coding for 6-phosphogluconate dehydrogenases which contain at position 329 or a comparable position of the amino acid sequence any amino acid other than L-valine, and to processes for the production of amino acids, preferably L-lysine and L-tryptophan, by fermentation using bacteria that contain these alleles.
    Type: Grant
    Filed: January 18, 2008
    Date of Patent: July 21, 2009
    Assignee: Degussa AG, Intellectural Property Management
    Inventors: Georg Thierbach, Brigitte Bathe, Natalie Schischka
  • Publication number: 20090170170
    Abstract: The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.
    Type: Application
    Filed: July 16, 2005
    Publication date: July 2, 2009
    Applicant: BASF Aktiengesellschaft
    Inventors: Jong-Soo Choi, Weol Kyu Jeong, Il Kwon Kim, Seong Han Lim, Heung-Shick Lee
  • Publication number: 20090170169
    Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the rspAB operon.
    Type: Application
    Filed: December 30, 2008
    Publication date: July 2, 2009
    Inventors: Dmitriy Vladimirovich Filippov, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner
  • Publication number: 20090137011
    Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the rcsA gene.
    Type: Application
    Filed: November 26, 2008
    Publication date: May 28, 2009
    Inventors: Dmitriy Vladimirovich Filippov, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner
  • Patent number: 7534590
    Abstract: A solution to be treated containing monatin and impurities is treated with a nonpolar resin having aromatic rings, whereby monatin is separated from the solution. Treatment with the nonpolar resin having aromatic rings results in efficient separation of monatin under a mild pH condition.
    Type: Grant
    Filed: December 27, 2005
    Date of Patent: May 19, 2009
    Assignee: AJINOMOTO Co., Inc.
    Inventors: Ken-ichi Mori, Masakazu Sugiyama, Tadashi Takemoto
  • Publication number: 20090104667
    Abstract: An L-amino acid is produced by culturing a microorganism which belongs to the family Enterobacteriaceae and is able to produce an L-amino acid, wherein the bacterium has been modified to enhance orotate phosphoribosyltransferase activity is enhanced, in a medium to produce and cause accumulation of an L-amino acid in the medium or cells, and collecting the L-amino acid from the medium or the cells.
    Type: Application
    Filed: October 27, 2008
    Publication date: April 23, 2009
    Inventors: Yoko Asakura, Yuri Nagai, Takuji Ueda
  • Publication number: 20090098621
    Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of a gene coding for sRNA.
    Type: Application
    Filed: September 18, 2008
    Publication date: April 16, 2009
    Inventors: Konstantin Vyacheslavovich Rybak, Aleksandra Yurievna Skorokhodova, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner, Tatyana Viktorovna Leonova, Yury Ivanovich Kozlov, Takuji Ueda, Vitaly Grigorievich Paraskevov
  • Publication number: 20090087886
    Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the sfmACDFH-fimZ cluster and/or the fimZ gene.
    Type: Application
    Filed: October 17, 2008
    Publication date: April 2, 2009
    Inventors: Dmitriy Vladimirovich Filippov, Vera Georgievna Doroshenko, Aleksandra Yurievna Skorokhodova, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner
  • Publication number: 20090081738
    Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to attenuate expression of the bolA gene.
    Type: Application
    Filed: July 31, 2007
    Publication date: March 26, 2009
    Inventors: Dmitriy Vladimirovich Filippov, Elvira Borisovna Voroshilova, Tatyana Viktorovna Leonova, Yuri Ivanovich Kozlov, Vitaly Grigorievich Paraskevov
  • Publication number: 20090068711
    Abstract: The invention relates to a method for the production of L-amino acids by fermentation of recombinant microorganisms of the Enterobacteriaceae family, characterized in that a) the microorganisms producing the desired L-amino acid and wherein the lamB-gene or nucleotide sequence coding for the gene product maltoporin is amplified, particularly overexpressed, are cultivated in a medium in conditions enabling the desired L-amino acid to be enriched in the medium or in cells, and b) the desired L-amino acid is isolated, wherein constituents of the fermentation broth and/or biomass remain in the entirety thereof or in parts thereof (=0 bis 100%) in the isolated product or are fully removed.
    Type: Application
    Filed: July 5, 2005
    Publication date: March 12, 2009
    Applicant: Degussa GmbH
    Inventor: Mechthild Rieping
  • Publication number: 20090053778
    Abstract: The present invention relates to microorganisms and methods for producing at least one sulfur-containing compound.
    Type: Application
    Filed: July 20, 2005
    Publication date: February 26, 2009
    Applicant: FRANCE TELECOM
    Inventors: Uwe Sauer, Jorg Mampel, Oskar Zelder, Andrea Herold, Corinna Klopprogge, Hartwig Schroder, Stefan Haefner
  • Patent number: 7485444
    Abstract: The invention relates to methods for the fermentative production of sulfur-containing fine chemicals, in particular L-methionine, by using bacteria which express a nucleotide sequence coding for a methionine synthase (metH) gene.
    Type: Grant
    Filed: April 16, 2003
    Date of Patent: February 3, 2009
    Assignee: Evonik Degussa GmbH
    Inventors: Burkhard Kröger, Oskar Zelder, Corinna Klopprogge, Hartwig Schröder, Stefan Häfner
  • Publication number: 20090029424
    Abstract: The present invention relates to the use of recombinant homoserine transsuccinylase enzymes with altered feedback sensitivity (MetA*) and possibly recombinant S-adenosyl methionine synthetase enzymes with reduced activity (MetK*) for the production of methionine, its precursors or derivatives thereof.
    Type: Application
    Filed: May 12, 2005
    Publication date: January 29, 2009
    Applicant: Metabolic Explorer
    Inventors: Gwenaelle Bestel-Corre, Michel Chateau, Rainer Figge, Celine Raynaud, Philippe Soucaille
  • Patent number: 7476531
    Abstract: There is provided a method for producing L-threonine, L-valine, L-proline, L-leucine, L-methionine and L-arginine using bacterium belonging to the genus Escherichia wherein L-amino acid productivity of the bacterium is enhanced by enhancing an activity of proteins coded by b2682 and b2683 genes, or protein coded by b1242 or b3434 gene.
    Type: Grant
    Filed: February 13, 2002
    Date of Patent: January 13, 2009
    Assignee: Ajinomoto Co., Inc.
    Inventors: Ekaterina Aleksandrovna Tabolina, Konstantin Vyacheslavovich Rybak, Evgeni Moiseevich Khourges, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner
  • Publication number: 20090004705
    Abstract: The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.
    Type: Application
    Filed: February 13, 2008
    Publication date: January 1, 2009
    Applicant: BASF Aktiengesellschaft
    Inventors: Burkhard Kroger, Oskar Zelder, Corinna Klopprogge, Hartwing Schroder, Stefan Haefner
  • Patent number: 7470525
    Abstract: There is provided a process for producing an optically active 2-alkylcysteine or a salt thereof, characterized by allowing cells of microorganism or treated products thereof having an activity of stereoselective hydrolysis of the amide bond of a 2-alkyl-L-cysteinamide or a salt thereof to act on a 2-alkylcysteinamide consisting of a mixture of D- and L-isomers or a salt thereof; and allowing the obtained 2-alkyl-L-cysteine and 2-alkyl-D-cysteinamide to react with an aldehyde or a ketone, or an acetal or ketal thereof, so as to derive therefrom a 4-alkylthiazolidine-4-carboxylic acid or a salt thereof and a 4-alkylthiazolidine-4-carboxamide or a salt thereof, thereby efficiently separating and obtaining a 2-alkyl-L-cysteine or a salt thereof, or a 2-alkyl-D-cysteine or a salt thereof. There is also provided a process for producing a 4-alkylthiazolidine-4-carboxylic acid or a salt thereof from the 2-alkylcysteine or a salt thereof.
    Type: Grant
    Filed: November 18, 2004
    Date of Patent: December 30, 2008
    Assignee: Mitsubishi Gas Chemical Company, Inc.
    Inventors: Yasushi Higuchi, Akinori Tanaka, Ryuji Hasemi, Masaki Sugita
  • Publication number: 20080318790
    Abstract: The present invention relates to a process for the production of methionine in an organism such as a microorganism, a non-human animal or plant. The invention furthermore relates to nucleic acid molecules, polypeptides, nucleic acid constructs, vectors, antisense molecules, antibodies, host cells, plant tissue, propagation material, harvested material, plants, microorganisms as well as agricultural compositions and to their use.
    Type: Application
    Filed: March 30, 2005
    Publication date: December 25, 2008
    Applicant: Metanomics GmbH
    Inventors: Marcus Ebneth, Piotr Puzio, Astrid Blau, Tilmann B. Walk, Volker Haake, Klaus Däschner, Gunnar Plesch
  • Publication number: 20080311632
    Abstract: A method of producing methionine, derivatives or precursors thereof comprising culturing a microorganism modified to enhance production of cysteine in a culture medium comprising a source of carbon and a source of sulfur and recovering methionine from the culture medium. A microorganism for fermentative production of methionine or its derivatives in which production of methionine or derivatives thereof, wherein the microorganism enhances production of cysteine.
    Type: Application
    Filed: January 4, 2006
    Publication date: December 18, 2008
    Inventors: Rainer Figge, Fabien Lux, Celine Raynaud, Michel Chateau, Philippe Soucaille
  • Publication number: 20080293109
    Abstract: The present invention relates to methods for converting plant cell wall polysaccharides into one or more products, comprising: treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into the one or more products.
    Type: Application
    Filed: July 14, 2008
    Publication date: November 27, 2008
    Applicant: Novozymes, Inc.
    Inventors: Randy Berka, Joel Cherry
  • Publication number: 20080286841
    Abstract: The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.
    Type: Application
    Filed: February 27, 2008
    Publication date: November 20, 2008
    Applicant: BASF Aktiengesellschaft
    Inventors: Burkhard Kroger, Oskar Zelder, Corinna Klopprogge, Hartwig Schroder, Stefan Haefner
  • Publication number: 20080274516
    Abstract: The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.
    Type: Application
    Filed: December 16, 2004
    Publication date: November 6, 2008
    Inventors: Burkhard Kroger, Oskar Zelder, Corinna Klopprogge, Hartwig Schroder, Stefan Haefner
  • Patent number: 7435420
    Abstract: The present invention discloses a method for therapeutically treating an animal, including a human, for psychosomatic, depressive and neuropsychiatric diseases, such as anxiety, depression, insomnia, schizophrenia, epilepsy, spasm and chronic pain. Administration of a suitable DP IV inhibitor causes the reduction of activity in the enzyme dipeptidyl peptidase (DP IV or CD 26) or of DP IV—like enzyme activity in the brain of mammals and leads as a causal consequence to a reduced degradation of the neuropeptide Y (NPY) and similar substrates by DP IV and DP IV-like enzymes. Such treatment will result in a reduction or delay in the decrease of the concentration of functionally active neuronal NPY (1-36).
    Type: Grant
    Filed: April 4, 2006
    Date of Patent: October 14, 2008
    Assignee: Probiodrug AG
    Inventors: Stephan von Hoersten, Ants Kask, Hans-Ulrich Demuth, Huu Phuc Nguyen, Susanne Kruber, Matthias Hoffmann
  • Patent number: 7432085
    Abstract: In a method for producing an L-amino acid by culturing a microorganism having an ability to produce an L-amino acid in a medium to produce and accumulate the L-amino acid in the medium and collecting the L-amino acid from the medium, a Gram-negative bacterium having the Entner-Doudoroff pathway and modified so that 6-phosphogluconate dehydratase activity or 2-keto-3-deoxy-6-phosphogluconate aldolase activity, or activities of the both are enhanced is used as the microorganism.
    Type: Grant
    Filed: November 18, 2005
    Date of Patent: October 7, 2008
    Assignee: Ajinomoto Co., Inc.
    Inventors: Yoshihiko Hara, Hiroshi Izui, Takahiro Asano, Yasuyuki Watanabe, Tsuyoshi Nakamatsu
  • Publication number: 20080194030
    Abstract: Disclosed herein are a method of preparing an L-methionine production strain by overexpressing proteins involved in L-methionine biosynthesis in an L-threonine production strain, a strain prepared by the method, and a method of producing L-methionine using the strain.
    Type: Application
    Filed: June 16, 2005
    Publication date: August 14, 2008
    Inventors: Young Hoon Park, Kwang Myung Cho, Yong-Uk Shin
  • Patent number: 7410789
    Abstract: A microorganism strain secreting S-adenosylmethionine having increased activity of the cmr (mdfA) gene product is provided.
    Type: Grant
    Filed: March 2, 2006
    Date of Patent: August 12, 2008
    Assignee: Wacker Chemie AG
    Inventors: Thomas Schlösser, Susanne Leonhartsberger, Renate Flinspach
  • Publication number: 20080176295
    Abstract: The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.
    Type: Application
    Filed: March 17, 2008
    Publication date: July 24, 2008
    Inventors: Oskar Zelder, Corinna Klopprogge, Burkhard Kroger, Hartwig Schroder, Stefan Haefner
  • Publication number: 20080166775
    Abstract: The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.
    Type: Application
    Filed: February 27, 2008
    Publication date: July 10, 2008
    Applicant: BASF Aktiengesellschaft
    Inventors: Burkhard Kroger, Oskar Zelder, Corinna Klopprogge, Hartwig Schroder, Stefan Haefner
  • Publication number: 20080166774
    Abstract: The invention relates to a method for producing an amino acid comprising culturing a microorganism of the genus Corynebacterium or Brevibacterium wherein said microorganism is partially or completely deficient in at least one of the gene loci of the group which is formed by otsAB, treZ and treS, and subsequent isolation of the amino acid from the culture medium.
    Type: Application
    Filed: January 28, 2008
    Publication date: July 10, 2008
    Applicant: BASF AG
    Inventors: Corinna Klopprogge, Oskar Zelder, Burkhard Kroger, Hartwig Schroder, Stefan Hafner, Wolfgang Liebl
  • Patent number: 7381549
    Abstract: The invention relates to methods for the fermentative production of sulfur-containing fine chemicals, in particular L-methionine, by using bacteria which express a nucleotide sequence coding for a methionine synthase (metH) gene.
    Type: Grant
    Filed: August 26, 2003
    Date of Patent: June 3, 2008
    Assignee: BASF Aktiengesellschaft
    Inventors: Burkhard Kröger, Oskar Zelder, Corinna Klopprogge, Hartwig Schröder, Stefan Häfner
  • Publication number: 20080118959
    Abstract: The invention relates to methods for the production of sulfur-containing fine chemicals, in particular L-methionine, by fermentation using bacteria in which a nucleotide sequence encoding an S-adenosylmethionine synthase (metK) gene is expressed.
    Type: Application
    Filed: May 23, 2003
    Publication date: May 22, 2008
    Inventors: Hartwig Schroder, Burkhard Kroger, Oskar Zelder, Corinna Klopprogge, Stefan Hafner
  • Publication number: 20080076163
    Abstract: L-cysteine is produced by culturing an Escherichia bacterium having L-cysteine producing ability and containing a gene encoding an O-acetylserine sulphydrylase B or MalY regulatory protein that is modified so that cysteine desulfhydrase activity is reduced or eliminated. The bacterium is cultured in a medium to produce and cause accumulation of L-cysteine in the medium, and collecting L-cysteine from the medium.
    Type: Application
    Filed: March 3, 2005
    Publication date: March 27, 2008
    Inventors: Hiroshi Takagi, Shigeru Nakamori, Masaru Wada, Hirotada Mori
  • Patent number: 7338790
    Abstract: The invention relates to mutants and alleles of the gnd gene from coryneform bacteria coding for 6-phosphogluconate dehydrogenases which contain at position 329 or a comparable position of the amino acid sequence any amino acid other than L-valine, and to processes for the production of amino acids, preferably L-lysine and L-tryptophan, by fermentation using bacteria that contain these alleles.
    Type: Grant
    Filed: September 16, 2005
    Date of Patent: March 4, 2008
    Assignee: Degussa AG, Intellectual Property Management
    Inventors: Georg Thierbach, Brigitte Bathe, Natalie Schischka
  • Patent number: 7311836
    Abstract: According to the process for producing amino acid or salt thereof in the present invention, in the adsorption step, an amino acid-containing aqueous solution is fed into a pressure tight column so that a free amino acid is adsorbed on a carbonate-type anion exchange resin packed in the pressure tight column. Subsequently, in the elution step, eluent liquid containing a hydrogen carbonate ion and/or a carbonate ion is injected into the pressure tight column in a pressurized state to elute the amino acid adsorbed on the anion exchange resin and simultaneously to regenarate the anion exchange resin into the carbonate-type. In the case of purifying an acidic amino acid, an aqueous ammonium carbonate solution is employed as the eluent liquid. In the case of purifying a neutral amino acid, an aqueous carbonic acid solution, an aqueous hydrogen carbonate solution, an aqueous ammonium hydrogen carbonate solution or an aqueous ammonium carbonate solution is employed as the eluent liquid.
    Type: Grant
    Filed: June 27, 2005
    Date of Patent: December 25, 2007
    Assignee: Ajinomoto Co., Inc.
    Inventors: Yasuhiro Kusunose, Kenji Takahashi
  • Patent number: 7312058
    Abstract: O-acetylserine, L-cysteine and sulphurous compounds derived therefrom may be produced using a bacterium belonging to the genus Escherichia which harbors a mutant feedback-resistant serine acetyltransferases in which the amino acid sequence corresponding to positions from 89 to 96 in a wild-type serine acetyltransferase is replaced with any one of the amino acid sequences shown in SEQ ID NOS: 4 to 9, and feedback inhibition by L-cysteine in the bacterium is desensitized.
    Type: Grant
    Filed: July 16, 2004
    Date of Patent: December 25, 2007
    Assignee: Ajinomoto Co., Ltd.
    Inventors: Tatsuki Kashiwagi, Yuko Kai, Kohki Ishikawa, Ei-ichiro Suzuki, Hiroshi Takagi, Mikhail Kharisovich Ziyatdinov, Ekaterina Igorevna Redkina, Mikhail Markovich Gusyatiner
  • Patent number: 7300777
    Abstract: The present invention relates to a mutated pyruvate carboxylase gene from Corynebacterium. The mutant pyruvate carboxylase gene encodes a pyruvate carboxylase enzyme which is resistant to feedback inhibition from aspartic acid. The present invention also relates to a method of replacing the wild-type pyruvate carboxylase gene in Corynebacterium with this feedback-resistant pyruvate carboxylase gene. The present invention further relates to methods of the production of amino acids, preferably lysine, comprising the use of this mutant pyruvate carboxylase enzyme in microorganisms.
    Type: Grant
    Filed: May 25, 2005
    Date of Patent: November 27, 2007
    Assignees: Archer-Daniels-Midland Company, Massachusetts Institute of Technology
    Inventors: Paul D. Hanke, Anthony J. Sinskey, Laura B. Willis, Stephane Guillouet
  • Patent number: 7282357
    Abstract: The invention relates to methods for the fermentative production of sulfur-containing fine chemicals, in particular L-methionine, by using bacteria which express a nucleotide sequence coding for a methionine synthase (metF) gene.
    Type: Grant
    Filed: August 26, 2003
    Date of Patent: October 16, 2007
    Assignee: BASF Aktiengesellschaft
    Inventors: Burkhard Kröger, Oskar Zelder, Corinna Kolpprogge, Hartwig Schröder, Stefan Häfner
  • Patent number: 7273920
    Abstract: The invention relates to novel compounds comprising a ubiquitination recognition element and a protein binding element. The invention also relates to the use of said compounds for modulating the level and/or activity of a target protein. The compounds are useful for the treatment of disease such as infections, inflammatory conditions, cancer and genetic diseases. The compounds are also useful as insecticides and herbicides.
    Type: Grant
    Filed: January 16, 2003
    Date of Patent: September 25, 2007
    Assignee: Wellstat BioCatalysis, LLC
    Inventors: John H. Kenten, Steven F. Roberts
  • Patent number: 7238502
    Abstract: The invention relates to methods for the fermentative production of sulfur-containing fine chemicals, in particular L-methionine, by using bacteria which express a nucleotide sequence coding for a methionine synthase (metA) gene.
    Type: Grant
    Filed: August 26, 2003
    Date of Patent: July 3, 2007
    Assignee: BASF Aktiengesellschaft
    Inventors: Burkhard Kröger, Oskar Zelder, Corinna Kolpprogge, Hartwig Schröder, Stefan Häfner
  • Patent number: 7211415
    Abstract: The present invention relates to a process for the production of L-amino acids by fermentation of recombinant microorganisms of the Enterobacteriaceae family, wherein a) the yfiD ORF and/or the pflB gene or nucleotide sequences coding for the gene products are overexpressed in the microorganisms producing the desired L-amino acid, and the microorganisms are cultured in a medium under conditions in which the desired L-amino acid is enriched in the medium or in the cells; and b) the desired L-amino acid is isolated, in a manner such that constituents of the fermentation broth and/or the biomass in its entirety or in portions (>0 to 100%) either remain in the isolated product or are completely removed.
    Type: Grant
    Filed: April 5, 2004
    Date of Patent: May 1, 2007
    Assignee: Degussa AG
    Inventors: Mechthild Rieping, Mike Farwick
  • Patent number: 7208631
    Abstract: A process for producing a 2-alkylcysteinamide, which comprises hydrolysis of a 4-alkylthiazolidine-4-carboxamide represented by the general formula (2) or a salt thereof: wherein R represents a lower alkyl group having 1–4 carbon atoms; and each of R1 and R2 independently represents hydrogen or a lower alkyl group having 1–4 carbon atoms, or R1 and R2 are linked together to form an alicyclic, structure having 4–7 carbon atoms, excluding the case where both R1 and R2 are hydrogen, to give a 2-alkylcysteinamide represented by the general formula (1) or a salt thereof wherein R represents a lower alkyl group having 1–4 carbon atoms. Cells of a microorganism or treated products thereof having activity of stereoselective hydrolysis of a 2-alkyl-L-cysteinamide are allowed to act on the compound represented by the general formula (1) to yield a 2-alkyl-L-cysteine.
    Type: Grant
    Filed: April 7, 2004
    Date of Patent: April 24, 2007
    Assignee: Mitsubishi Gas Chemical Company, Inc.
    Inventors: Yasushi Higuchi, Akinori Tanaka, Ryuji Hasemi
  • Patent number: 7195897
    Abstract: The invention relates to a homoserine transsuccinylase, which exhibits reduced sensitivity towards L-methionine or SAM in comparison with a homoserine transsuccinylase wild-type enzyme, whereby the latter comprises an amino acid sequence containing a TyrGlnXaaThrPro sub-sequence, the Thr of said sub-sequence lying between positions 285 and 310 of the amino acid sequence and position 1 being filled by the starter methionine. The inventive homoserine transsuccinylase is characterized in that in comparison with the wild-type enzyme at least 2 amino acids are modified, said modification taking place in the Thr of the sub-sequence or in the C-terminal.
    Type: Grant
    Filed: October 16, 2003
    Date of Patent: March 27, 2007
    Assignee: Consortium für elektrochemische Industrie GmbH
    Inventors: Susanne Leonhartsberger, Christoph Winterhalter, Kerstin Pfeiffer, Brigitte Bauer
  • Patent number: 7192747
    Abstract: A methane-utilizing microorganism capable of producing L-amino acid, for example, bacteria belonging to type I, type X or type II in the taxonomic categorization methane-utilizing bacteria such as Methylomonas albus, Methylococcus capsulatus and Methylosinus trichosporium, is cultivated in a culture medium in contact with gas containing methane which is the main source of carbon, to allow the L-amino acid to be produced and accumulated in the medium, and the L-amino acid is collected from the medium.
    Type: Grant
    Filed: December 4, 2002
    Date of Patent: March 20, 2007
    Assignee: Ajinomoto, Co. Inc.
    Inventors: Yukiko Ono, Hisashi Yasueda, Yoshio Kawahara, Shinichi Sugimoto
  • Patent number: 7166436
    Abstract: The present invention relates to a method of simultaneously identifying and determining the levels of expression of cysteine-containing proteins in normal and perturbed cells, a method for proteomic analysis, a process for preparing fusion proteins, and compounds and reagents related thereto.
    Type: Grant
    Filed: August 1, 2002
    Date of Patent: January 23, 2007
    Assignee: Syngenta Participations, AG
    Inventors: Paul Haynes, Jing Wei, John Yates, Nancy Andon
  • Patent number: 7160570
    Abstract: The present invention relates to a method for the manufacture of a food material containing high quantities of cysteine. In this method, a yeast extract is prepared from yeast cells that contains ?-glutamylcysteine and the extract is concentrated by controlling the temperature to be not greater than 60° C. to prepare a food material in a liquid form, wherein the concentration of solids is at least 10% and the resulting food material is maintained at 70 to 130° C.
    Type: Grant
    Filed: March 10, 2004
    Date of Patent: January 9, 2007
    Assignee: Ajinomoto Co., Inc.
    Inventors: Hiroaki Nishiuchi, Yuki Nishimori, Yasushi Nishimura
  • Patent number: 7160703
    Abstract: The present invention provides nucleotide sequences from Coryneform bacteria which code for the PtsI protein and a process for the fermentative preparation of amino acids using bacteria in which the ptsI gene is enhanced.
    Type: Grant
    Filed: June 13, 2003
    Date of Patent: January 9, 2007
    Assignee: Degussa AG
    Inventors: Bettina Moeckel, Stephan Hans, Natalie Schischka, Walter Pfefferle
  • Patent number: 7157251
    Abstract: The present invention relates to the processes of racemization and deprotection of special N-protected amino acids in the acylase/racemase system for the total conversion of special N-protected racemic amino acids into optically pure amino acids.
    Type: Grant
    Filed: September 2, 2003
    Date of Patent: January 2, 2007
    Assignee: Degussa AG
    Inventors: Andreas Bommarius, Karlheinz Drauz, Stefan Verseck
  • Patent number: 7148047
    Abstract: L-cysteine is produced by culturing a coryneform bacterium in which intracellular serine acetyltransferase activity is increased by transformation with a gene coding for serine acetyltransferase, such modification of an expression control sequence of a gene coding for serine acetyltransferase that expression of the gene in a cell should be enhanced and for forth, preferably in which L-cysteine decomposition system is further suppressed, in a medium to produce and accumulate L-cysteine in culture and collecting the L-cysteine from the culture.
    Type: Grant
    Filed: February 1, 2002
    Date of Patent: December 12, 2006
    Assignee: Ajinomoto Co., Inc.
    Inventors: Hiroshi Takagi, Masaru Wada, Shigeru Nakamori
  • Patent number: 7118775
    Abstract: The present invention relates to a method for producing a cysteine-rich food material by maintaining a food material containing ?-glutamylcysteine at a ratio of at least 1 wt % to the solid content thereof at a temperature ranging from 50 to 120° C. and at a pH ranging from 1 to 7. This process is conducted in the absence of a sugar and in the presence of water. The present invention also relates to a method for producing a cysteine-rich food material by reacting the food material with a ?-glutamyl peptide hydrolase at a pH ranging from 3 to 9 and at a temperature ranging from 15 to 70° C. in the present of water. Further, the present invention relates to a method for producing a flavor-enhancing material for use in food, food products obtained by these processes, and yeast cells or extracts for use in food products.
    Type: Grant
    Filed: December 2, 2002
    Date of Patent: October 10, 2006
    Assignee: Ajinomoto Co., Inc.
    Inventors: Masanori Kohmura, Yasushi Nishimura, Koh-ichiro Sano, Hirokazu Kawaguchi, Gaku Hibino, Reiko Sugimoto, Hiroaki Nishiuchi, Hidehiko Wakabayashi, Yoichi Ueda, Minori Ishiguro, legal representative, Tatsuya Ishiguro, legal representative, Shouji Ishiguro, legal representative, Youhei Ishiguro, legal representative, Kyousuki Ishiguro, deceased