Methionine; Cysteine; Cystine Patents (Class 435/113)
  • Publication number: 20120164698
    Abstract: The invention relates to a method for preparing organic-chemical compounds, characterized in that the following steps are carried out: a) fermentation of a microorganism secreting an L-amino acid, which microorganism contains an overexpressed polynucleotide coding for a polypeptide having polyphosphate-dependent NAD+ kinase activity, in a fermentation medium, to form a fermentation broth, b) accumulation of said compound in said fermentation broth and/or in the cells of said microorganism. The invention relates to a method for preparing organic-chemical compounds by fermentation of a microorganism in which a polypeptide having polyphosphate-dependent NAD+ kinase is overexpressed.
    Type: Application
    Filed: June 18, 2010
    Publication date: June 28, 2012
    Applicant: EVONIK DEGUSSA GMBH
    Inventors: Volker F. Wendisch, Steffen Lindner, Brigitte Bathe, Wilfried Claes
  • Patent number: 8187850
    Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the ybiV gene.
    Type: Grant
    Filed: January 30, 2008
    Date of Patent: May 29, 2012
    Assignee: Ajinomoto Co., Inc.
    Inventors: Konstantin Vyacheslavovich Rybak, Marina Evgenievna Sheremet'eva, Aleksandra Yurievna Skorokhodova, Tatyana Viktorovna Leonova, Yury Ivanovich Kozlov, Vitaly Grigorievich Paraskevov, legal representative
  • Publication number: 20120123158
    Abstract: The present invention relates to a method for increasing L-methionine productivity and organic acid productivity. More particularly, the present invention relates to a method which involves adding a mixture containing methyl mercaptan and dimethyl sulfide at a appropriate ratio to O-acetyl homoserine or O-succinyl homoserine and to an enzyme having an activity of converting methionine precursor into L-methionine, so as to perform an enzyme reaction, to thereby improve the conversion rate of L-methionine and organic acid from the L-methionine precursor, and thus increasing L-methionine yield as compared to conventional method.
    Type: Application
    Filed: February 26, 2010
    Publication date: May 17, 2012
    Applicant: CJ CHEILJEDANG CORPORATION
    Inventors: So Young Kim, Yong Uk Shin, In Kyung Heo, Hyun Ah Kim, Ju Eun Kim, Chang Il Seo, Sung Kwang Son, Sang Mok Lee, Sung Hoo Jhon, Han Jin Lee, Kwang Ho Na, Il Chul Kim
  • Patent number: 8178322
    Abstract: A method is described for producing an L-amino acid or a nucleic acid by culturing a microorganism having an ability to produce the L-amino acid or nucleic acid in a liquid medium in a fermentation tank containing a stirring impeller, and optionally adding seed crystals to the medium as required to produce and accumulate crystals of the L-amino acid or nucleic acid in the medium, and collecting crystals of the L-amino acid or nucleic acid from the culture. The power density of the stirring impeller is controlled to be 2.4 kW/m3 or lower after either precipitation of the crystals or addition of the seed crystals.
    Type: Grant
    Filed: August 14, 2009
    Date of Patent: May 15, 2012
    Assignee: Ajinomoto Co., Inc.
    Inventors: Masayuki Araki, Yusuke Takahashi, Akihiro Watanabe, Fumito Ohnishi, Takahiro Asano, Kazuya Kondo, Wataru Hibino, Shintaro Iwatani, Satoshi Okutani
  • Publication number: 20120115186
    Abstract: The present invention relates to a process for the production of one or more fermentation product from a sugar composition, comprising the following steps: a) fermentation of the sugar composition in the presence of a yeast belonging to the genera Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Kloeckera, Schwanniomyces or Yarrowia, and b) recovery of the fermentation product, wherein the yeast comprises the genes araA, araB and araD and the sugar composition comprises glucose, galactose and arabinose.
    Type: Application
    Filed: July 6, 2010
    Publication date: May 10, 2012
    Applicant: DSM IP ASSETS B.V.
    Inventors: Paul Klaassen, Gijsberdina Pieternella Van Suylekom, Bianca Elisabeth Maria Gielesen, Nicolette Jasmijn Broers, Beate Wiedemann, Wilhelmus Theodorus Antonius Maria De Laat
  • Patent number: 8163532
    Abstract: The present invention relates to microorganisms and methods for producing methionine by reactivation of the MetH enzyme.
    Type: Grant
    Filed: May 27, 2009
    Date of Patent: April 24, 2012
    Assignee: Evonik Degussa GmbH
    Inventors: Oskar Zelder, Hartwig Schröder, Corinna Klopprogge, Andrea Herold, Stefan Haefner, R. Rogers Yocum, Thomas A. Patterson, Mark Williams
  • Patent number: 8148117
    Abstract: The present invention relates to microorganisms and processes for the efficient preparation of L-amino acids such as L-methionine. In particular, the present invention relates to microorganisms and processes in which the formation and/or accumulation of homolanthionine in the methionine pathway is reduced and/or prevented.
    Type: Grant
    Filed: October 23, 2006
    Date of Patent: April 3, 2012
    Assignee: Evonik Degussa GmbH
    Inventors: Oskar Zelder, Andrea Herold, Corinna Klopprogge, Hartwig Schröder, Stefan Haefner, Elmar Heinzle, Christoph Wittmann, Jens Kroemer, Janice G. Pero, R. Rogers Yocum, Thomas A. Patterson, Mark Williams, Theron Herman
  • Patent number: 8143031
    Abstract: The present invention claims an isolated polypeptide having L-amino-acid-N-acyl transferase enzymatic activity and a modified microorganism in which this enzyme is overexpressed. Substrates of said enzyme include mainly methionine and their derivatives or analogs. Overexpression in sulphur-containing amino acid producing microorganisms permits the production of large amounts of N-acylated sulphur-containing amino acids. The isolation of the N-acylated sulphur-containing amino acids from the fermentation medium is also claimed.
    Type: Grant
    Filed: February 12, 2009
    Date of Patent: March 27, 2012
    Assignee: Metabolic Explorer
    Inventors: Rainer Figge, Guillaume Barbier, Gwénaëlle Bestel-Corre
  • Patent number: 8114649
    Abstract: L-cysteine is produced by culturing an Escherichia bacterium having L-cysteine producing ability and containing a gene encoding an O-acetylserine sulphydrylase B or MalY regulatory protein that is modified so that cysteine desulfhydrase activity is reduced or eliminated. The bacterium is cultured in a medium to produce and cause accumulation of L-cysteine in the medium, and collecting L-cysteine from the medium.
    Type: Grant
    Filed: December 10, 2009
    Date of Patent: February 14, 2012
    Assignee: Ajinomoto Co., Inc.
    Inventors: Hiroshi Takagi, Shigeru Nakamori, Masaru Wada, Hirotada Mori
  • Patent number: 8114639
    Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the sfmACDFH-fimZ cluster and/or the fimZ gene.
    Type: Grant
    Filed: October 17, 2008
    Date of Patent: February 14, 2012
    Assignee: Ajinomoto Co., Inc.
    Inventors: Dmitriy Vladimirovich Filippov, Vera Georgievna Doroshenko, Aleksandra Yurievna Skorokhodova, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner
  • Publication number: 20120015409
    Abstract: The present invention provides a method of improving efficiency of a fermentative production of an L-amino acid.
    Type: Application
    Filed: February 9, 2010
    Publication date: January 19, 2012
    Applicant: KYOWA HAKKO BIO CO., LTD.
    Inventors: Kazuhiko Tabata, Akihiro Senoo
  • Publication number: 20110318792
    Abstract: The object of the present invention is to provide a microbial mutant capable of producing S-adenosylmethionine (hereinafter referred to as SAM) in a large amount with no change in growth characteristics and a method of efficiently producing SAM by incubating the microbial mutant. The method of producing S-adenosylmethionine comprises incubating a microorganism having a loss or a reduction in the function of an enzyme relating to synthesis of phosphatidylcholine and recovering S-adenosylmethionine accumulated in the culture.
    Type: Application
    Filed: August 14, 2007
    Publication date: December 29, 2011
    Applicant: ISHIHARA SANGYO KAISHA, LTD.
    Inventors: Toshio Ohsuga, Kuni Fushikida
  • Patent number: 8076106
    Abstract: A process for producing high yields of enantioselective amino acids and chiral amines by reacting a keto acid or ketone and an amino acid donor in the presence of a transaminase biocatalyst to produce a keto acid by-product and an amino acid or amine product. Further reacting the keto acid by-product with a peroxide to increase the yield of additional amino acid or amine product.
    Type: Grant
    Filed: August 6, 2009
    Date of Patent: December 13, 2011
    Assignee: Richmond Chemical Corporation
    Inventors: Ian Fotheringham, Nicholas Oswald
  • Patent number: 8071331
    Abstract: A method of producing amino acid metal chelates includes producing an amino acid ligand by enzymatically hydrolyzing bacterial cells, and reacting the amino acid ligand with a metal cation.
    Type: Grant
    Filed: June 3, 2009
    Date of Patent: December 6, 2011
    Assignee: Ajinomoto U.S.A., Inc.
    Inventors: Paul Summer, Shinya Tachibana, Randall Vos
  • Publication number: 20110269185
    Abstract: The present invention provides compositions and methods designed to increase value output of a fermentation reaction that yields a first product, intended for commercialization, such as ethanol, and a fermentation residual used, for example, as animal feed. The methods involve using microorganisms in the fermentation process that have been modified so as to yield a residual having greater value that a residual produced in the process by a microorganism not so modified. In particular, the present invention contemplates using microorganisms in a fermentation process that have been modified to increase production of a nutrient, such as an essential amino acid, thereby reducing the need to supplement the nutrient in the animal's diet. The present invention also provides a modified fermentation residual of higher commercial value. Also provided in the present invention are complete animal feeds, nutritional supplements comprising the subject ferment residuals.
    Type: Application
    Filed: April 27, 2011
    Publication date: November 3, 2011
    Inventor: Peter R. David
  • Patent number: 8048651
    Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i.e., fructose-1,6-bisphosphatase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of increasing the expression of fructose-1,6-bisphosphatase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.
    Type: Grant
    Filed: July 31, 2009
    Date of Patent: November 1, 2011
    Assignee: BASF SE
    Inventors: Oskar Zelder, Corinna Klopprogge, Hartwig Schroder, Stefan Haefner, Burkhard Kröger, Patrick Kiefer, Elmar Heinzle, Christoph Wittmann
  • Publication number: 20110229937
    Abstract: Isolated nucleic acid molecules, designated MR nucleic acid molecules, which encode novel MR proteins from Corynebacterium glutamicum are described which are involved in biosynthesis of a fine chemical, e.g., methionine biosynthesis. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing MR nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides methods of producing methionine from microorganisms, e.g., C. glutamicum, which involve culturing recombinant microorganisms which overexpress or underexpress at least one MR molecule of the invention under conditions such that methionine is produced. Also featured are methods of producing a fine chemical, e.g., methionine, which involve culturing recombinant microorganisms having selected MR genes deleted or mutated under conditions such that the fine chemical, e.g., methionine, is produced.
    Type: Application
    Filed: January 31, 2011
    Publication date: September 22, 2011
    Applicant: Evonik Degussa GMBH
    Inventors: Markus Pompejus, Burkhard Kröger, Hartwig Schröder, Oskar Zelder, Gregor Haberhauer, Stefan Haefner, Corrinna Klopprogge
  • Publication number: 20110223284
    Abstract: The present invention provides compositions and methods designed to increase value output of a fermentation reaction. In particular, the present invention provides a business method of increasing value output of a fermentation plant. The present invention also provides a modified fermentation residual of higher commercial value. Also provided in the present invention are complete animal feeds, nutritional supplements comprising the subject ferment residuals. Further provided by the present invention is a method of performing fermentation, a modified fermentative microorganism and a genetic vehicle for modifying such microorganism.
    Type: Application
    Filed: October 14, 2010
    Publication date: September 15, 2011
    Applicant: Ambrozea, Inc.
    Inventor: Peter R. David
  • Patent number: 8017362
    Abstract: The present invention provides a method for producing L-methionine by culturing a microorganism in a medium to produce and accumulate L-methionine in the medium, and collecting the L-methionine from the medium, where the microorganism is deficient in a repressor of L-methionine biosynthesis system and has L-methionine productivity.
    Type: Grant
    Filed: September 8, 2009
    Date of Patent: September 13, 2011
    Assignee: Ajinomoto Co., Inc.
    Inventors: Yoshihiro Usuda, Osamu Kurahashi
  • Patent number: 8012722
    Abstract: L-amino acids such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, and L-cysteine are produced by culturing in a medium a bacterium having an L-amino acid-producing ability and wherein the bacterium has been modified so that the phosphotransacetylase activity is enhanced.
    Type: Grant
    Filed: July 25, 2008
    Date of Patent: September 6, 2011
    Assignee: Ajinomoto Co., Inc.
    Inventors: Akito Chinen, Hisashi Yasueda, Jun Nakamura
  • Publication number: 20110212496
    Abstract: An L-amino acid is produced by culturing a bacterium belonging to the family Enterobacteriaceae, which has an L-amino acid-producing ability and inherently has a native activity of a glucose dehydrogenase that uses pyrroloquinoline quinone as a coenzyme, but has been modified so that the activity of the glucose dehydrogenase is reduced, in a medium, and collecting the L-amino acid from the medium.
    Type: Application
    Filed: March 1, 2011
    Publication date: September 1, 2011
    Inventors: Rie Takikawa, Yoshihiko Hara, Gen Nonaka, Kazuhiro Takumi
  • Patent number: 8008048
    Abstract: The present invention provides a bacterium belonging to the family Enterobacteriaceae which has L-cysteine-producing ability and has been modified to decrease the activity of the protein encoded by the yhaM gene. This bacterium is cultured in a medium, and L-cysteine, L-cystine, their derivatives, or a mixture thereof is collected from the medium.
    Type: Grant
    Filed: March 4, 2009
    Date of Patent: August 30, 2011
    Assignee: Ajinomoto Co., Inc.
    Inventors: Gen Nonaka, Kazuhiro Takumi
  • Publication number: 20110207184
    Abstract: The present invention relates to a microorganism producing L-methionine precursor, O-acetylhomoserine, and a method of producing L-methionine precursor using the microorganism.
    Type: Application
    Filed: January 3, 2011
    Publication date: August 25, 2011
    Applicant: CJ CHEILJEDANG CORPORATION
    Inventors: Chul Ha Kim, So Young Kim, Young Uk Shin, Hye Won Um, Jin Sook Chang, Young Wook Cho, Han Jin Lee, In Kyung Heo, Chang II Seo, Kwang Ho Na
  • Publication number: 20110207183
    Abstract: The present invention is directed to a method utilizing a microorganism with reduced isocitrate dehydrogenase activity for the production of fine chemicals. Said fine chemicals may be amino acids, monomers for polymer synthesis, sugars, lipids, oils, fatty acids or vitamins and are preferably amino acids of the aspartate family, especially methionine or lysine, or derivatives of said amino acids, especially cadaverine. Furthermore, the present invention relates to a recombinant microorganism having a reduced isocitrate dehydrogenase activity in comparison to the initial microorganism and the use of such microorganisms in producing fine chemicals such as aspartate family amino acids and their derivatives.
    Type: Application
    Filed: April 28, 2009
    Publication date: August 25, 2011
    Applicant: BASF SE
    Inventors: Andrea Herold, Hartwig Schröder, Weol Kyu Jeong, Corinna Klopprogge, Oskar Zelder, Stefan Haefner, Ulrike Richter, Judith Becker, Christoph Wittmann
  • Publication number: 20110183383
    Abstract: Described herein are microorganisms that produce methionine and related products from endogenous genes in a transsulfuration pathway, as well as from exogenous genes providing a direct sulfhydrylation pathway. Novel genes that are useful for methionine and SAMe production are disclosed.
    Type: Application
    Filed: April 11, 2007
    Publication date: July 28, 2011
    Applicant: CJ Cheijedang Corporation
    Inventors: Brian Brazeau, Jin-Sook Chang, Kwang Myung Cho, Young Wook Cho, Mervyn Desouza, Holly J. Jessen, So-Young Kim, Wei Niu, Fernando A. Sanchez-Riera, Yong-Uk Shin, Hyewon Um
  • Publication number: 20110177566
    Abstract: A method for producing an L-amino acid is described using a bacterium of the Enterobacteriaceae family, wherein the bacterium contains a protein which is able to confer resistance to growth inhibition by L-cysteine.
    Type: Application
    Filed: January 11, 2011
    Publication date: July 21, 2011
    Inventors: Ekaterina Alekseevna Savrasova, Natalia Viktorovna Stoynova, Gen Nonaka, Shunsuke Yamazaki, Kazuhiro Takumi
  • Patent number: 7951565
    Abstract: The present invention relates to a process for the production of methionine in an organism such as a microorganism, a non-human animal or plant. The invention furthermore relates to nucleic acid molecules, polypeptides, nucleic acid constructs, vectors, antisense molecules, antibodies, host cells, plant tissue, propagation material, harvested material, plants, microorganisms as well as agricultural compositions and to their use.
    Type: Grant
    Filed: March 30, 2005
    Date of Patent: May 31, 2011
    Assignee: Metanomics GmbH
    Inventors: Marcus Ebneth, Piotr Puzio, Astrid Blau, Tilmann B. Walk, Volker Haake, Klaus Däschner, Gunnar Plesch
  • Publication number: 20110117614
    Abstract: The present invention is directed to a method utilizing a microorganism with reduced isocitrate dehydrogenase activity for the production of methionine.
    Type: Application
    Filed: April 27, 2009
    Publication date: May 19, 2011
    Applicant: EVONIK DEGUSSA GMBH
    Inventors: Oskar Zelder, Hartwig Schöder, Corinna Klopprogge, Andrea Herold, Stefan Haefner
  • Publication number: 20110111466
    Abstract: The present invention relates to a transformed microorganism producing an L-amino acid using sucrose as a main carbon source, and a method for producing an L-amino acid using the same.
    Type: Application
    Filed: February 11, 2010
    Publication date: May 12, 2011
    Applicant: CJ Cheiljedang Corporation
    Inventors: Jae Yeong Ju, Kwang Ho Lee, Hyun Ae Bae, Hyo Jin Kim, Keun Chul Lee, Young Bin Hwang, Kyu Soo Cho, Hye Min Park, Hyun Ah Kim
  • Publication number: 20110053227
    Abstract: The invention relates to a process for preparing L-amino acids by fermenting recombinant microorganisms of the Enterobacteriaceae family, characterized in that a) the desired L-amino acid-producing microorganisms, in which the yjcG-ORF, or nucleotide sequences or alleles encoding the gene product, is/are enhanced, in particular overexpressed, is cultured in a medium under conditions under which the desired L-amino acid is accumulated in the medium or in the cells, and b) the desired L-amino acid is isolated, with, where appropriate, constituents of the fermentation broth, and/or the biomass remaining in its/their entirety or in portions (from ?0 to 100%) in the isolated product or being removed completely.
    Type: Application
    Filed: January 22, 2010
    Publication date: March 3, 2011
    Inventor: Nicole DUSCH
  • Patent number: 7892798
    Abstract: Isolated nucleic acid molecules, which encode novel metabolic regulatory proteins from Corynebacterium glutamicum are described. These nucleic acid molecules are involved in the biosynthesis of a fine chemical, e.g., methionine biosynthesis. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing metabolic regulatory nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides methods of producing methionine from microorganisms, e.g., C. glutamicum, which involve culturing recombinant microorganisms which overexpress or underexpress at least one metabolic regulatory molecule of the invention under conditions such that methionine is produced. Also featured are methods of producing a fine chemical, e.g., methionine, which involve culturing recombinant microorganisms having selected metabolic regulatory genes deleted or mutated under conditions such that the fine chemical, e.g.
    Type: Grant
    Filed: November 29, 2002
    Date of Patent: February 22, 2011
    Assignee: Evonik Degussa GmbH
    Inventors: Markus Pompejus, Burkhard Kröger, Hartwig Schröder, Oskar Zelder, Gregor Haberhauer, Stefan Haefner, Corrinna Klopprogge
  • Patent number: 7888077
    Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to attenuate expression of the kefB gene.
    Type: Grant
    Filed: November 5, 2007
    Date of Patent: February 15, 2011
    Assignee: Ajinomoto., Inc.
    Inventors: Dmitriy Vladimirovich Filippov, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner
  • Publication number: 20110033902
    Abstract: L-Cysteine, L-cystine, a derivative or precursor thereof, or a mixture thereof is produced by culturing a bacterium belonging to the family Enterobacteriaceae, which has L-cysteine-producing ability and has been modified so that the activity of a protein encoded by a tolC gene, for example, a protein defined in the following (a) or (b), is increased in a medium, and by collecting L-cysteine, L-cystine, a derivative or precursor thereof, or a mixture thereof from the medium: (a) a protein comprising the amino acid sequence of SEQ ID NO: 2, (b) a protein comprising the amino acid sequence of SEQ ID NO: 2, but wherein one or several amino acid residues are substituted, deleted, inserted or added, increase of which activity in the bacterium improves the ability of the bacterium to produce L-cysteine.
    Type: Application
    Filed: August 18, 2010
    Publication date: February 10, 2011
    Inventors: Gen Nonaka, Hiroshi Takagi, Iwao Ohtsu
  • Publication number: 20110033896
    Abstract: The present invention relates to a method for manufacturing an aqueous glucose solution from the starch components of Triticeae grains, for example from rye, triticale or in particular wheat grains. The invention also relates to a glucose-based fermentation method for manufacturing organic compounds in which the glucose manufactured for fermentation is produced from the starch components of Triticeae grains by way of a method according to the invention.
    Type: Application
    Filed: April 9, 2009
    Publication date: February 10, 2011
    Applicant: BASF SE
    Inventors: Matthias Boy, Stephan Freyer, Julia Brodersen
  • Publication number: 20100330623
    Abstract: The present invention relates generally to compositions and methods useful for, inter alia, production of commercial biologic products such as amino acids. More specifically, the present invention relates to genetically modified strains of microorganisms and the use thereof for the production of commercial products. The present invention also provides, inter alia, novel isolated DNA, nucleic acid, vectors and reduced genome bacteria.
    Type: Application
    Filed: February 19, 2009
    Publication date: December 30, 2010
    Applicant: SCARAB GENOMICS LLC
    Inventors: Frederick R. Blattner, Sun Chang Kim, Jun Hyoung Lee
  • Patent number: 7850852
    Abstract: According to the process for producing amino acid or salt thereof in the present invention, in the adsorption step, an amino acid-containing aqueous solution is fed into a pressure tight column so that a free amino acid is adsorbed on a carbonate-type anion exchange resin packed in the pressure tight column. Subsequently, in the elution step, eluent liquid containing a hydrogen carbonate ion and/or a carbonate ion is injected into the pressure tight column in a pressurized state to elute the amino acid adsorbed on the anion exchange resin and simultaneously to regenarate the anion exchange resin into the carbonate-type. In the case of purifying an acidic amino acid, an aqueous ammonium carbonate solution is employed as the eluent liquid. In the case of purifying a neutral amino acid, an aqueous carbonic acid solution, an aqueous hydrogen carbonate solution, an aqueous ammonium hydrogen carbonate solution or an aqueous ammonium carbonate solution is employed as the eluent liquid.
    Type: Grant
    Filed: August 24, 2007
    Date of Patent: December 14, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Yasuhiro Kusunose, Kenji Takahashi
  • Patent number: 7846887
    Abstract: Cysteine derivatives of formula (I) wherein R1-R7 are as defined in the description, and their use as malodor counteractant. Furthermore, a process of their production and consumer products comprising them are described.
    Type: Grant
    Filed: June 18, 2008
    Date of Patent: December 7, 2010
    Assignee: Givaudan SA
    Inventors: Felix Flachsmann, Markus Gautschi, Thomas McGee, Richard P. Sgaramella
  • Publication number: 20100279362
    Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the ybiV gene.
    Type: Application
    Filed: January 30, 2008
    Publication date: November 4, 2010
    Inventors: Konstantin Vyacheslavovich Rybak, Marina Evgenievna Sheremet'eva, Aleksandra Yurievna Skorokhodova, Tatyana Viktorovna Leonova, Yury Ivanovich Kozlov, Vitaly Grigorievich Paraskevov
  • Publication number: 20100267094
    Abstract: The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.
    Type: Application
    Filed: June 28, 2010
    Publication date: October 21, 2010
    Inventors: Yury Ivanovich Kozlov, Akito Chinen, Hiroshi Izui, Yoshihiko Hara, Hisashi Yasueda, Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Joanna Yosifovna Katashkina
  • Publication number: 20100255544
    Abstract: The invention relates to coryneform bacteria which, instead of the singular copy of an open reading frame (ORF), gene or allele naturally present at the particular desired site (locus), have at least two copies of the open reading frame (ORF), gene or allele in question, preferably in tandem arrangement, and optionally at least a third copy of the open reading frame (ORF), gene or allele in question at a further gene site, and processes for the preparation of chemical compounds by fermentation of these bacteria.
    Type: Application
    Filed: September 3, 2009
    Publication date: October 7, 2010
    Applicant: Evonik Degussa GmbH
    Inventors: Brigitte Bathe, Caroline Kreutzer, Bettina Mockel, Georg Thierbach
  • Publication number: 20100248311
    Abstract: Process for the production of methionine or its derivatives by culturing a microorganism in an appropriate culture medium comprising a source of carbon and a source of sulfur. The microorganism and/or the culture medium are modified in such way that the methionine/carbon source yield is increased. The isolation of methionine or its derivates from the fermentation medium is also described.
    Type: Application
    Filed: September 25, 2008
    Publication date: September 30, 2010
    Applicant: METABOLIC EXPLORER
    Inventor: Rainer FIGGE
  • Publication number: 20100233765
    Abstract: The present invention provides a bacterium belonging to the family Enterobacteriaceae, which is able to produce L-cysteine, and has been modified to decrease activity of the YdjN protein, or the activities of the YdjN and the FliY protein. This bacterium is cultured in a medium, and L-cysteine, L-cystine, a derivative or precursor thereof, or a mixture of these can be collected from the medium.
    Type: Application
    Filed: March 11, 2010
    Publication date: September 16, 2010
    Inventor: Gen Nonaka
  • Patent number: 7790424
    Abstract: Disclosed herein are a method of preparing an L-methionine production strain by overexpressing proteins involved in L-methionine biosynthesis in an L-threonine production strain, a strain prepared by the method, and a method of producing L-methionine using the strain.
    Type: Grant
    Filed: June 16, 2005
    Date of Patent: September 7, 2010
    Assignee: CJ Cheiljedang Corporation
    Inventors: Young Hoon Park, Kwang Myung Cho, Yong-Uk Shin, Hyewon Um
  • Publication number: 20100221792
    Abstract: An L-amino acid is produced by culturing a bacterium belonging to the family Enterobacteriaceae, which is able to produce the L-amino acid, and is modified so that the activity of ribonuclease G is decreased in a medium containing glycerol as the carbon source, and collecting the L-amino acid from the culture.
    Type: Application
    Filed: March 30, 2010
    Publication date: September 2, 2010
    Inventors: YURI NAGAI, YOSHIHIRO USUDA
  • Patent number: 7785846
    Abstract: The present invention relates to a process for producing methionine by fermentation, a process for isolating the methionine formed, the methionine-containing biomass produced in isolation, use thereof for producing a feedstuff or feed supplement, and also to the use of the isolated methionine for producing foodstuffs or feedstuffs or food supplements or feed supplements.
    Type: Grant
    Filed: December 17, 2004
    Date of Patent: August 31, 2010
    Assignee: Evonik Degussa GmbH
    Inventors: Matthias Boy, Daniela Klein, Hartwig Schröder
  • Publication number: 20100216196
    Abstract: The present invention provides a bacterium belonging to the family Enterobacteriaceae, which is able to produce L-cysteine and has been modified to increase the activity of the protein encoded by the dsbA gene. This bacterium is cultured in a medium, and L-cysteine, L-cystine, a derivative or precursor thereof or a mixture thereof can be collected from the medium.
    Type: Application
    Filed: February 24, 2010
    Publication date: August 26, 2010
    Inventors: Gen Nonaka, Hiroshi Takagi, Iwao Ohtsu, Natthawut Wiriyathanawudhiwong, Takeshi Nakatani
  • Publication number: 20100209977
    Abstract: A bacterium belonging to the family Enterobacteriaceae, which has an ability to produce an amino acid such as L-cysteine and has been modified to have specific mutation in the yeas gene, is cultured in a medium, and the L-amino acid is collected from the medium.
    Type: Application
    Filed: February 16, 2010
    Publication date: August 19, 2010
    Inventors: Kazuhiro Takumi, Gen Nonaka
  • Publication number: 20100203592
    Abstract: The invention provides a process for producing glutathione or ?-glutamylcysteine by culturing in a medium a microorganism with a higher activity of a protein having an activity to transport intracellular glutathione to the outside of cells, and a higher activity of a protein involved in glutathione or ?-glutamylcysteine biosynthesis, compared with that of the parent strain, forming and accumulating glutathione or ?-glutamylcysteine in the medium, and recovering the glutathione or ?-glutamylcysteine from the culture.
    Type: Application
    Filed: April 4, 2008
    Publication date: August 12, 2010
    Applicant: KYOWA HAKKO BIO CO., LTD.
    Inventors: Kazuhiko Tabata, Yoshiyuki Yonetani
  • Patent number: 7771976
    Abstract: The present invention provides a method for producing a non-aromatic L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to attenuate expression of the csrA gene.
    Type: Grant
    Filed: July 31, 2007
    Date of Patent: August 10, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Andrey Yurievich Gulevich, Danila Vadimovich Zimenkov, Elena Vitalievna Klyachko, Tatyana Viktorovna Leonova, Yury Ivanovich Koslov, Vitaly Grigorievich Paraskevov, legal representative
  • Publication number: 20100197934
    Abstract: The present invention provides a simple industrial process for producing an L- or D-optically active ?-methylcysteine derivative or its salt, which is a useful pharmaceutical intermediate, from readily available, inexpensive raw materials. In a process for producing an L- or D-optically active ?-methylcysteine derivative or its salt, a racemic N-carbamoyl-?-methylcysteine derivative or its salt is D-selectively cyclized with hydantoinase to produce a D-5-methyl-5-thiomethylhydantoin derivative or its salt and an N-carbamoyl-?-methyl-L-cysteine derivative or its salt, which are then subjected to deprotection of the amino group and the sulfur atom, and hydrolysis.
    Type: Application
    Filed: April 9, 2010
    Publication date: August 5, 2010
    Applicant: KANEKA CORPORATION
    Inventors: Takahiro Ohishi, Hirokazu Nanba, Masanobu Sugawara, Masashi Izumida, Tatsuya Honda, Kohei Mori, Satohiro Yanagisawa, Nobuo Nagashima, Kenji Inoue