Containing At Least Two Hetero Rings Bridged Or Fused Among Themselves Or Bridged Or Fused With A Common Carbocyclic Ring System, (e.g., Rifamycin, Etc.) Patents (Class 435/119)
  • Patent number: 10429400
    Abstract: The present invention relates to methods of testing medications to evaluate authenticity and identify counterfeits. The testing methods employ a reactive system comprising a solvent and an acid. Optionally, the reactive system can also comprise an organometallic agent. The testing methods can provide rapid results verifying the authenticity of an antimalarial medication. Further the test method can provide clear results that can be implemented and interpreted without special training, anywhere in the world. The test methods can offer quantitative and qualitative results. The test methods are based on reactions that yield different colors where the color can indicate the presence of an active ingredient, and the intensity of the color can indicate the concentration of the active ingredient.
    Type: Grant
    Filed: May 7, 2015
    Date of Patent: October 1, 2019
    Assignees: South Dakota Board of Regents, Rochester Institute of Technology
    Inventors: Daniel Heglund, Andrew Olson, Scott Williams, Neal Hodges, Joseph A. Marshall
  • Patent number: 10287545
    Abstract: Provided are a Streptomyces (Streptomyces hygroscopicus) HS7523 and a method for preparing milbemycin A3 by culturing the Streptomyces. The Streptomyces (Streptomyces hygroscopicus) HS7523 is deposited in “China General Microbiological Culture Collection Center” with an accession number of CGMCC No. 9672 on Sep. 16, 2014.
    Type: Grant
    Filed: March 25, 2016
    Date of Patent: May 14, 2019
    Assignee: Zhejiang Hisun Pharmaceutical Co., Ltd.
    Inventors: Yun Teng, Meidong Xu, Meiyi Mo, Zhihui He, Zhengjie Chen, Lianiqing Jiang, Hua Bai
  • Patent number: 10208322
    Abstract: The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention.
    Type: Grant
    Filed: September 28, 2016
    Date of Patent: February 19, 2019
    Assignee: California Institute of Technology
    Inventors: Pedro S. Coelho, Eric M. Brustad, Frances H. Arnold, Zhan Wang, Jared C. Lewis
  • Patent number: 10111856
    Abstract: The present invention relates to a composition for, inter alia, preventing or treating a climacteric disease, or preventing or improving a climacteric skin symptom comprising a soybean extract comprising coumestrol as an active ingredient, and more specifically relates to a composition for, inter alia, preventing or treating a climacteric disease, or preventing or improving a climacteric skin symptom comprising a germinated and fermented soybean extract comprising coumestrol as an active ingredient.
    Type: Grant
    Filed: May 7, 2014
    Date of Patent: October 30, 2018
    Assignee: AMOREPACIFIC CORPORATION
    Inventors: Hyun Jung Shin, Hyun Woo Jeong, Ji Hae Lee, Dae Jin Min, Dae Bang Seo, Sang Jun Lee
  • Patent number: 9630994
    Abstract: Synthetic nanostructures, polypeptides that are useful, for example, in making synthetic nanostructures, and methods for using such synthetic nanostructures are disclosed herein.
    Type: Grant
    Filed: November 3, 2015
    Date of Patent: April 25, 2017
    Assignee: University of Washington
    Inventors: David Baker, Jacob B. Bale, Neil P. King, William H. Sheffler, Daniel Ellis
  • Patent number: 9631181
    Abstract: The present disclosure relates to non-naturally occurring monooxygenase polypeptides useful for preparing prazole compounds, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
    Type: Grant
    Filed: July 15, 2016
    Date of Patent: April 25, 2017
    Assignee: Codexis, Inc.
    Inventors: Yong Koy Bong, Michael D. Clay, Steven J. Collier, Benjamin Mijts, Michael Vogel, Xiyun Zhang, Jun Zhu, Jovana Nazor, Derek J. Smith, Shiwei Song
  • Patent number: 9617573
    Abstract: The present disclosure provides engineered transaminase polypeptides useful for the synthesis of chiral amine compounds under industrially relevant conditions. The disclosure also provides polynucleotides encoding the engineered transaminase polypeptides, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases for the production of chiral amine compounds.
    Type: Grant
    Filed: February 24, 2014
    Date of Patent: April 11, 2017
    Assignee: Codexis, Inc.
    Inventors: Martina Quintanar-Audelo, Ellen Eberhard, Jovana Nazor, Derek Smith, Cuixia Wang
  • Patent number: 9523107
    Abstract: The invention is directed to immobilized transaminases and methods of making and using them.
    Type: Grant
    Filed: February 24, 2014
    Date of Patent: December 20, 2016
    Assignee: Merck Sharp & Dohme Corp.
    Inventors: Matthew D. Truppo, Michel Journet, Hallena Strotman, Jonathan P. McMullen, Shane T. Grosser
  • Patent number: 9399762
    Abstract: The disclosure generally relates to the fields of synthetic organic chemistry. In particular, the present disclosure relates to methods and systems for the imidation of sulfides.
    Type: Grant
    Filed: February 18, 2015
    Date of Patent: July 26, 2016
    Assignee: California Institute of Technology
    Inventors: Christopher C. Farwell, John A. McIntosh, Frances H. Arnold
  • Patent number: 9315785
    Abstract: An isolated novel polynucleotide comprising a nucleotide sequence encoding at least one polypeptide involved in biosynthesis of pyripyropene A, a recombinant vector comprising the polynucleotide and a transformant comprising the polynucleotide are disclosed. By the present invention, a pyripyropene A biosynthetic gene useful for production of a novel pyripyropene analog, improvement of productivity of a pyripyropene A-producing bacterium, production of an insecticidal agent for microorganisms, creation of a plant resistant to insect pests or the like are provided.
    Type: Grant
    Filed: September 10, 2013
    Date of Patent: April 19, 2016
    Assignee: MEIJI SEIKA PHARMA CO., LTD.
    Inventors: Hiroyuki Anzai, Kentaro Yamamoto, Mariko Tsuchida, Kazuhiko Oyama, Masaaki Mitomi
  • Patent number: 9273332
    Abstract: The present invention has its object to provide a method for producing an L-amino acid comprising reacting a keto acid with an amino acid dehydrogenase and an enzyme having coenzyme regenerating ability to convert to a L-amino acid, wherein a coenzyme is added in two or more portions in the reaction. The method of the present invention enables efficient production of an L-amino acid useful as a synthetic intermediate such as a pharmaceutical intermediate with high optical purity by an enzymatic reductive amination independent of the purity of the keto acid used as a substrate.
    Type: Grant
    Filed: October 11, 2007
    Date of Patent: March 1, 2016
    Assignee: Kaneka Corporation
    Inventors: Hiroyuki Kanamaru, Makoto Ueda, Hirokazu Nanba
  • Patent number: 9169504
    Abstract: There is provided a method for culturing a microorganism in which a particular polynucleotide or a recombinant vector comprising it/them is introduced with an intermediate compound necessary for biosynthesis of pyripyropene A. The method of the present invention allows for the production of pyripyropene.
    Type: Grant
    Filed: January 19, 2011
    Date of Patent: October 27, 2015
    Assignee: MEIJI SEIKA PHARMA CO., LTD.
    Inventors: Hiroyuki Anzai, Kentaro Yamamoto, Kazuhiko Oyama, Mariko Tsuchida, Kimihiko Goto, Masaaki Mitomi
  • Patent number: 9139821
    Abstract: The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents. The present disclosure provides engineered polypeptides having transaminase activity, polynucleotides encoding the polypeptides, methods of the making the polypeptides, and methods of using the polypeptides for the biocatalytic conversion of ketone substrates to amine products. The present enzymes have been engineered to have one or more residue differences as compared to the amino acid sequence of the naturally occurring transaminase of Vibrio fluvialis. In particular, the transaminases of the present disclosure have been engineered for efficient formation of chiral tryptamine derivatives from its corresponding prochiral ketone substrates.
    Type: Grant
    Filed: March 22, 2013
    Date of Patent: September 22, 2015
    Assignee: Codexis, Inc.
    Inventors: Jovana Nazor, Derek Smith, Michael Crowe, Shiwei Song, Steven J. Collier
  • Patent number: 9090608
    Abstract: A pharmaceutical composition comprising a phenyltetrazole derivative of formula (I) or a pharmaceutical acceptable salt thereof is effective in preventing or treating osteoporosis, obesity, diabetes, or hyperlipidemia, by regulating protein TAZ. wherein, A is ethyl or n-butyl; R1 is methyl, —CH2OH, —CO2CH3, —CH2F, —CH(OCH3)2, —CH2OC(?O)CH3, styryl or —CH2OCH2SCH3; R2 is H, Br, —CO2CH3, phenyl, pyridin-2-yl, pyridin-3-yl, or styryl; R3 is H or methyl; X is CH or N; and P is H or —CH(CH3)OCH2CH3.
    Type: Grant
    Filed: January 4, 2013
    Date of Patent: July 28, 2015
    Assignee: KOREA RESEARCH INSTITUTE OF CHEMICAL TECHNOLOGY
    Inventors: Nak Jeong Kim, Myung-Ae Bae, Sung-Eun Yoo, Namsook Kang, Jung Nyoung Heo, Kyu Yang Yi, Jee Hee Suh, Sung Youn Chang, Eun Sook Hwang, Jeong-Ho Hong
  • Patent number: 9085788
    Abstract: The present invention provides a convenient and efficient process for the preparation of enantiomerically enriched, deuterated secondary alcohols without reducing deuterium incorporation.
    Type: Grant
    Filed: September 1, 2011
    Date of Patent: July 21, 2015
    Assignee: Concert Pharmaceuticals, Inc.
    Inventors: Ramanaiah C. Kanamarlapudi, Steven A. Weissman, Emerich Eisenreich, Xuejun Liu
  • Patent number: 9074233
    Abstract: The present invention provides a process for the preparation of enantiomerically enriched, deuterated secondary alcohols of Formula 1-A by employing ketoreductases or carbonyl reductases without reducing deuterium incorporation.
    Type: Grant
    Filed: September 1, 2011
    Date of Patent: July 7, 2015
    Assignee: Concert Pharmaceuticals, Inc.
    Inventors: Ramanaiah C. Kanamarlapudi, Steven A. Weissman, Emerich Eisenreich, Xuejun Liu
  • Patent number: 9062334
    Abstract: The present invention provides a method for producing PQQ using a bacterium belonging to the genera Methylobacterium or Hyphomicrobium, which has been modified to enhance the expression of pqq gene cluster and/or gene(s) encoding a precursor for PQQ biosynthesis.
    Type: Grant
    Filed: August 26, 2013
    Date of Patent: June 23, 2015
    Assignee: AJINOMOTO CO., INC.
    Inventors: Evgeniy Rodionovich Gak, Natalya Vasilievna Gorshkova, Irina L'vovna Tokmakova
  • Publication number: 20150118719
    Abstract: The present disclosure provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents. The present disclosure provides engineered proline hydroxylase biocatalysts, polynucleotides encoding the biocatalysts. methods of their preparation, and processes for preparing hydroxylated compounds using these engineered biocatalysts.
    Type: Application
    Filed: May 7, 2013
    Publication date: April 30, 2015
    Inventors: Haibin Chen, Yong Koy Bong, Fabien L. Cabirol, Anupam Gohel Prafulchandra, Tao Li, Jeffrey C. Moore, Martina Quintanar-Audelo, Yang Hong, Steven J. Collier, Derek Smith
  • Patent number: 9017974
    Abstract: There is herein described a nanovesicle comprising a bilayer of porphyrin-phospholipid conjugates. Each porphyrin-phospholipid conjugate comprises one porphyrin, porphyrin derivative or porphyrin analog covalently attached to a lipid side chain at one of the sn-1 or the sn-2 positions of one phospholipid. Further, the nanovesicle has a defined regioisomeric ratio of sn-1:sn-2 porphyrin-phospholipid conjugates.
    Type: Grant
    Filed: May 25, 2012
    Date of Patent: April 28, 2015
    Assignee: University Health Network
    Inventors: Gang Zheng, Jonathan Lovell
  • Publication number: 20150099879
    Abstract: Methods for producing phycotoxins from natural sources, wherein the phycotoxins have a definite compositional profile are described herein. In one embodiment, the phycotoxins are produced by cyanobacteria. In one embodiment, the phycotoxins are produced by continuously culturing cyanobacteria under strictly controlled conditions in order to produce a definite compositional profile. In another embodiment, organic nutrients are added to the culture that allows for higher concentrations of neosaxitoxin and saxitoxin or gonyaulatoxins 2 and 3 per weight of the algae. The phycotoxins are isolated primarily from the bacteria but can also be isolated from the culture medium. In one embodiment, the cyanobacteria produce only neosaxitoxin and saxitoxin in a ratio of about 6:1, 5:1, 4:1, or 3:1. In a preferred embodiment, the amount of saxitoxin is less than 20% by weight of the total amount of neosaxitoxin and saxitoxin produced. In another embodiment, the cyanobacteria produce only GTX2 and GTX 3.
    Type: Application
    Filed: December 15, 2014
    Publication date: April 9, 2015
    Inventor: Marcelo Santiago Logas Gonzalez
  • Patent number: 8993262
    Abstract: The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention.
    Type: Grant
    Filed: February 20, 2014
    Date of Patent: March 31, 2015
    Assignee: California Institute of Technology
    Inventors: Pedro S. Coelho, Eric M. Brustad, Frances H. Arnold, Zhan Wang, Jared C. Lewis
  • Publication number: 20150079642
    Abstract: The present invention provides a novel method for producing rapamycin by submerged fermentation which comprises cultivating Streptomyces hygroscopicus (CBS 773.72) and mutants thereof. The present invention provides a high yielding mutant culture MTCC5681 from Streptomyces hygroscopicus CBS 773.72. This culture is capable of producing rapamycin more efficiently than the cultures Streptomyces hygroscopicus from sources like ATCC, NRRL etc which have been reported so far.
    Type: Application
    Filed: April 12, 2012
    Publication date: March 19, 2015
    Applicant: Natco Pharma Limited
    Inventors: Baby Rani Polavarapu, Suneel Kumar Battula, Kali Satya Bhujanga Rao Adibhatla, Venkaiah Chowdary Nannapaneni
  • Publication number: 20150079640
    Abstract: The present disclosure relates to methods of using transaminase polypeptides in the synthesis of chiral amines from prochiral ketones.
    Type: Application
    Filed: November 19, 2014
    Publication date: March 19, 2015
    Inventors: Gregory Hughes, Paul N. Devine, Fred J. Fleitz, Brendan T. Grau, John Limanto, Christopher Savile, Emily Mundorff
  • Patent number: 8980586
    Abstract: An object of the present invention is to provide a novel compound useful as an antifungal substance and a method for producing the compound.
    Type: Grant
    Filed: October 22, 2012
    Date of Patent: March 17, 2015
    Assignees: The Ritsumeikan Trust, National University Corporation Shizuoka University
    Inventors: Nobutaka Imamura, Kazuya Nakagawa, Shinji Tokuyama
  • Publication number: 20150072383
    Abstract: The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents. The present disclosure provides engineered polypeptides having transaminase activity, polynucleotides encoding the polypeptides, methods of the making the polypeptides, and methods of using the polypeptides for the biocatalytic conversion of ketone substrates to amine products. The present enzymes have been engineered to have one or more residue differences as compared to the amino acid sequence of the naturally occurring transaminase of Vibrio fluvialis. In particular, the transaminases of the present disclosure have been engineered for efficient formation of chiral tryptamine derivatives from its corresponding prochiral ketone substrates.
    Type: Application
    Filed: March 22, 2013
    Publication date: March 12, 2015
    Inventors: Jovana Nazor, Derek Smith, Michael Crowe, Shiwei Song, Steven J. Collier
  • Publication number: 20150050704
    Abstract: The present invention is directed to the biosynthetic pathway for a nonribosomal peptide synthetase (NRPS) derived drug and analogs thereof. The invention also discloses polynucleotide sequences useful for heterologous expression in a convenient microbial host for the synthesis of the NRPS derived drug.
    Type: Application
    Filed: August 25, 2014
    Publication date: February 19, 2015
    Inventors: David H. Sherman, Garth D. Ehrlich, Benjamin Janto, Robert M. Williams, Christopher M. Rath
  • Patent number: 8957207
    Abstract: Methods for producing phycotoxins from natural sources, wherein the phycotoxins have a definite compositional profile are described herein. In one embodiment, the phycotoxins are produced by cyanobacteria. In one embodiment, the phycotoxins are produced by continuously culturing cyanobacteria under strictly controlled conditions in order to produce a definite compositional profile. In another embodiment, organic nutrients are added to the culture that allows for higher concentrations of neosaxitoxin and saxitoxin or gonyaulatoxins 2 and 3 per weight of the algae. The phycotoxins are isolated primarily from the bacteria but can also be isolated from the culture medium. In one embodiment, the cyanobacteria produce only neosaxitoxin and saxitoxin in a ratio of about 6:1, 5:1, 4:1, or 3:1. In a preferred embodiment, the amount of saxitoxin is less than 20% by weight of the total amount of neosaxitoxin and saxitoxin produced. In another embodiment, the cyanobacteria produce only GTX2 and GTX 3.
    Type: Grant
    Filed: September 23, 2011
    Date of Patent: February 17, 2015
    Assignee: Proteus S.A.
    Inventor: Marcelo Santiago Lagos González
  • Publication number: 20150044735
    Abstract: The biosynthesis of fungal bicyclo[2.2.2]diazaoctane indole alkaloids with a wide spectrum of biological activities have attracted increasing interest. Their intriguing mode of assembly has long been proposed to feature a non-ribosomal peptide synthetase, a presumed intramolecular Diels-Alderase, a variant number of prenyltransferases, and a series of oxidases responsible for the diverse tailoring modifications of their cyclodipeptide-based structural core. Until recently, the details of these biosynthetic pathways have remained largely unknown due to lack of information on the fungal derived biosynthetic gene clusters. Herein, we report a comparative analysis of four natural product metabolic systems of a select group of bicyclo[2.2.2]diazaoctane indole alkaloids including (+)/(?)-notoamide, paraherquamide and malbrancheamide, in which we propose an enzyme for each step in the biosynthetic pathway based on deep annotation and on-going biochemical studies.
    Type: Application
    Filed: April 3, 2013
    Publication date: February 12, 2015
    Inventors: Shengying Li, Krithika Anand Srinivasan, Robert M. Williams, David H. Sherman
  • Publication number: 20150004659
    Abstract: The disclosure relates to a nucleic acid molecule isolated from a Papaver somniferum cultivar that produces the opiate alkaloid noscapine which comprises 10 genes involved in the biosynthesis of opiate alkaloids.
    Type: Application
    Filed: March 12, 2013
    Publication date: January 1, 2015
    Applicant: GlaxoSmithKline Australia Pty Limited
    Inventors: Thilo Winzer, Ian Alexander Graham, Tracy Carol Walker
  • Publication number: 20150005490
    Abstract: Provided is a novel strain permitting genetic engineering of green sulfur bacteria that synthesize bacteriochlorophyll e. Isolated green sulfur bacterium Chlorobaculum limnaeum strain RK-j-1 with accession number NITE BP-1202; isolated transformed Chlorobaculum limnaeum strain obtained by transformation of strain RK-j-1, preferably Chlorobaculum limnaeum dbchU strain with accession number NITE BP-1203 wherein bchU gene is disrupted; a production method of bacteriochlorophyll f containing a step of cultivating Chlorobaculum limnaeum strain dbchU; and bacteriochlorophyll f obtained by the aforementioned production method.
    Type: Application
    Filed: February 12, 2013
    Publication date: January 1, 2015
    Inventors: Jiro Harada, Masato Noguchi, Hitoshi Tamiaki
  • Publication number: 20140377813
    Abstract: The invention provides a microbial eukaryotic cell capable of utilizing C5 sugars, in particular xylose. Another objective of the invention is to provide an improved protein sequence to enable eukaryotic cells to degrade C5 sugars. The present invention thus provides protein comprising an amino acid sequence having at least 75% identity, preferably 80% identity, most preferably 90% identity, most highly preferably 95% identity to SEQ ID NO. 2 or SEQ ID NO. 8 and having xylose-isomerase activity in a eukaryotic cell.
    Type: Application
    Filed: February 7, 2013
    Publication date: December 25, 2014
    Inventors: Zdravko Dragovic, Christian Gamauf, Christoph Reisinger, Ulrich Kettling
  • Publication number: 20140370556
    Abstract: The invention relates to a new method for preparing (R)-praziquantel. In the invention, by taking advantage of the high stereo selectivity, site selectivity and region selectivity of an enzyme, an intermediate of a pure optical and chiral (R)-praziquantel are obtained by means of the dynamic kinetic resolution of an enantiomer from the synthesized racemate or derivatives thereof, and the (R)-praziquantel is obtained by using various conventional and mature organic chemical reactions with higher yield. The method of the invention has the potential advantages of easily available raw materials, low cost, environmentally safer process and convenience for large-scale production. Also, the purity of the end product can be more than 98%. By adopting the invention, the quality of the product is improved and a basis for developing high quality of active pharmaceutical ingredients and formulations is established, and thus the pending industrial problem of purifying praziquantel over 30 years becomes solvable.
    Type: Application
    Filed: August 28, 2014
    Publication date: December 18, 2014
    Inventor: Mingxin Qian
  • Publication number: 20140370555
    Abstract: The invention relates to a new method for preparing (R)-praziquantel. In the invention, by taking advantage of the high stereo selectivity, site selectivity and region selectivity of an enzyme, an intermediate of a pure optical and chiral (R)-praziquantel are obtained by means of the dynamic kinetic resolution of an enantiomer from the synthesized racemate or derivatives thereof, and the (R)-praziquantel is obtained by using various conventional and mature organic chemical reactions with higher yield. The method of the invention has the potential advantages of easily available raw materials, low cost, environmentally safer process and convenience for large-scale production. Also, the purity of the end product can be more than 98%. By adopting the invention, the quality of the product is improved and a basis for developing high quality of active pharmaceutical ingredients and formulations is established, and thus the pending industrial problem of purifying praziquantel over 30 years becomes solvable.
    Type: Application
    Filed: August 28, 2014
    Publication date: December 18, 2014
    Inventor: Mingxin Qian
  • Publication number: 20140371456
    Abstract: Provided are processes for the synthesis of amino analogues from ketone starting materials.
    Type: Application
    Filed: April 21, 2014
    Publication date: December 18, 2014
    Inventors: Brian C. Austad, Adilah Bahadoor, Jitendra D. Belani, Somarajannair Janardanannair, Sheldon L. Wallerstein, Gregg F. Keaney, Priscilla L. White, Charles W. Johannes
  • Patent number: 8900830
    Abstract: A process for efficiently producing optically active succinimide derivatives as key intermediates of (3R)-2?-(4-bromo-2-fluorobenzyl)spiro{pyrrolidine-3,4?(1?H)-pyrrolo[1,2-a]pyrazine}-1?,2,3?,5(2?H)-tetraone, which comprises the following reaction steps, and the step 2 is performed by using a non-animal-derived enzyme.
    Type: Grant
    Filed: July 28, 2011
    Date of Patent: December 2, 2014
    Assignees: Sumitomo Dainippon Pharma Co., Ltd., Kyowa Hakko Bio Co., Ltd.
    Inventors: Shinji Kita, Kazuya Tsuzaki, Eitora Yamamura
  • Patent number: 8895271
    Abstract: The present disclosure relates to non-naturally occurring monooxygenase polypeptides useful for preparing prazole compounds, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
    Type: Grant
    Filed: December 8, 2010
    Date of Patent: November 25, 2014
    Assignee: Codexis, Inc.
    Inventors: Yong Koy Bong, Michael D. Clay, Steven J. Collier, Benjamin Mijts, Michael Vogel, Xiyun Zhang, Jun Zhu, Jovana Nazor, Derek Smith, Shiwei Song
  • Patent number: 8889380
    Abstract: The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
    Type: Grant
    Filed: September 5, 2012
    Date of Patent: November 18, 2014
    Assignee: Codexis, Inc.
    Inventors: Christopher Savile, Emily Mundorff, Jeffrey Moore, Paul N. Devine, Jacob M. Janey
  • Patent number: 8883462
    Abstract: A triprenyl phenol compound represented by the following formula (II) and (III) and having a thrombolysis-enhancing activity, and an efficient method for producing the triprenyl phenol compound. In formula (II) and (III), R1 represents an aromatic group having as a substituent or as a part of a substituent at least one member selected from the group consisting of a carboxyl group, a hydroxyl group, a sulfonic acid group and a secondary amino group, or an aromatic group which comprises a secondary amino group and may comprise nitrogen; R4 in the general formula (III) represents an aromatic amino acid residue represented by the general formula (III-1) below; X represents —CHY—(CH3)2Z; and Y and Z are respectively —H or —OH or together form a single bond: (III-1) wherein R5 represents a hydroxyl group which may be present or absent; and n represents an integer of 0 or 1.
    Type: Grant
    Filed: January 4, 2012
    Date of Patent: November 11, 2014
    Assignee: Tokyo University of Agriculture and Technology TLO Co., Ltd.
    Inventors: Keiji Hasumi, Yoshikazu Kitano, Hideo Ohishi, Haruki Koide, Keiko Hasegawa, Ritsuko Narasaki
  • Patent number: 8877501
    Abstract: The present invention relates to the production of alkaloids from Liliaceae cell culture.
    Type: Grant
    Filed: June 18, 2010
    Date of Patent: November 4, 2014
    Assignee: Phyton Holdings, LLC
    Inventors: Venkatesh Srinivasan, Jennifer Alford, Beth Slusar-Place, Charles Swindell, Michael Horn, Khisal Alvi, Gilbert Gorr
  • Publication number: 20140315896
    Abstract: This invention relates to the field of biologically active compounds and specifically to phaeofungins, of the general formula (I) or a pharmacologically acceptable salt, solvate, hydrate or a pharmacologically acceptable formulation thereof, pharmaceutical compositions comprising these compounds, methods for the production of the phaeofungins and their use as fungicide, antibiotic as well as antitumor agent.
    Type: Application
    Filed: June 15, 2012
    Publication date: October 23, 2014
    Applicant: Leibniz-Institut fuer Naturstoff-Forschung und Infektionsbiologie E.V. Hans-Knoell-Institut (HKI)
    Inventors: Axel Brakhage, Hans-Martin Dahse, Christian Hertweck, Hans-Wilhelm Nuetzmann, Kirstin Scherlach
  • Publication number: 20140309438
    Abstract: An object of the present invention is to provide a novel compound useful as an antifungal substance and a method for producing the compound.
    Type: Application
    Filed: October 22, 2012
    Publication date: October 16, 2014
    Applicants: THE RITSUMEIKAN TRUST, NATIONAL UNIVERSITY CORPORATION SHIZUOKA UNIVERSITY
    Inventors: Nobutaka Imamura, Kazuya Nakagawa, Shinji Tokuyama
  • Patent number: 8841096
    Abstract: The invention relates to a method for the amination of at least one keto group in a multicyclic ring system comprising at least one keto group into an amino group, using at least one enzyme E having transaminase activity.
    Type: Grant
    Filed: January 13, 2010
    Date of Patent: September 23, 2014
    Assignee: Evonik Degussa GmbH
    Inventors: Volker Sieber, Katrin Grammann, Broder Ruehmann, Thomas Haas, Jan Christoph Pfeffer, Kai Doderer, Claudia Rollmann, Arne Skerra, Christian Rausch, Alexandra Lerchner
  • Publication number: 20140273109
    Abstract: Aspects of the invention include host cells that are engineered to produce benzylisoquinoline alkaloids (BIAs). The host cells include heterologous coding sequences for a variety of enzymes involved in synthetic pathways from starting compounds to BIAs of the host cell. Also provided are methods of producing the BIAs of interest by culturing the host cells under culture conditions that promote expression of enzymes encoded by the heterologous coding sequences of the host cells. Aspects of the invention further include compositions, e.g., host cells, starting compounds and kits, etc., that find use in methods of the invention.
    Type: Application
    Filed: March 14, 2014
    Publication date: September 18, 2014
    Inventors: Christina D. Smolke, Catherine Thodey, Isis Trenchard, Stephanie Galanie
  • Patent number: 8835487
    Abstract: The invention provides methods for treating multiple sclerosis by administering biotin. The invention also provides methods for treating sequalae after multiple sclerosis attacks by administering biotin.
    Type: Grant
    Filed: October 4, 2012
    Date of Patent: September 16, 2014
    Assignee: Assistance Publique Hopitaux De Paris
    Inventor: Frédéric Sedel
  • Publication number: 20140256003
    Abstract: Provided are R-praziquantel preparation methods, which utilize the characteristics of biological enzyme of strong stereoselectivity, site-selectivity, and regioselectivity, of high resolution efficiency, of mild reaction conditions, and of simple operations to catalyze the hydrolysis of a certain enantiomer in a chemically synthesized racemate or a derivative, thus acquiring a mixture of reacted and unreacted optical isomers. R-praziquantel prepared by the method can have a purity of 98% or more.
    Type: Application
    Filed: October 26, 2012
    Publication date: September 11, 2014
    Applicant: Suzhou Tongli Biomedical Co., Ltd.
    Inventor: Mingxin Qian
  • Patent number: 8829205
    Abstract: The present invention relates to a method for preparing coumestrol, comprising a step in which beans are germinated and a step in which the beans are fermented, and to coumestrol prepared by the method.
    Type: Grant
    Filed: January 31, 2011
    Date of Patent: September 9, 2014
    Assignee: Amorepacific Corporation
    Inventors: Hee Young Jeon, Dae Bang Seo, Si Young Cho, Sang Jun Lee
  • Publication number: 20140227729
    Abstract: The invention provides isolated nucleic acid encoding one or more gene products that allow for degradation of caffeine and related structures, and for preparation of intermediates in that catabolic pathway. Further provided are methods of using one or more of those gene products.
    Type: Application
    Filed: October 18, 2012
    Publication date: August 14, 2014
    Applicant: University of Iowa Research Foundation Iowa Centers for Enterprise
    Inventors: Venkiteswaran Subramanian, Michael Tai-Man Louie, Ryan Summers
  • Publication number: 20140220457
    Abstract: The present invention relates generally to substrates for making polymers and methods for making polymers. The present invention also relates generally to polymers and devices comprising the same.
    Type: Application
    Filed: February 5, 2014
    Publication date: August 7, 2014
    Applicant: Avertica, Inc.
    Inventor: Nicholas Brendan Duck
  • Publication number: 20140206889
    Abstract: [Problem] The present invention addresses the problem of providing an anti-trypanosomal drug having a novel skeleton, in order to solve issues that occur with conventional technologies. [Solution] The present invention is based on the discovery of a microbe that produces an anti-trypanosomal drug having a novel skeleton. Specifically, this invention provides: a compound indicated by formula (I) having a trypanosomal inhibitory activity; an analog thereof; a production method therefor; and a Lechevalieria sp. Kl0-0216 strain that produces said compound.
    Type: Application
    Filed: August 31, 2012
    Publication date: July 24, 2014
    Applicant: THE KITASATO INSTITUTE
    Inventors: Satoshi Omura, Yoko Takahashi, Takuji Nakashima, Kazuhiko Otoguro, Kazuro Shiomi, Masato Iwatsuki, Atsuko Matsumoto
  • Publication number: 20140201868
    Abstract: The present invention relates to fungi of Acremonium spp, wherein said fungi are purified or isolated from plants of the Brachiaria-Urochloa complex and wherein, when said fungi are inoculated into a plant, said plant has improved resistance to diseases and/or pests relative to an uninocualated control plant. The present invention also relates to plants inoculated with such fungi, products produced by the fungi and related genes, proteins and methods.
    Type: Application
    Filed: June 1, 2012
    Publication date: July 17, 2014
    Applicant: AGRICULTURE VICTORIA SERVICES PTY LTD
    Inventors: German Carlos Spangenberg, Kathryn Michaela Guthridge