Containing At Least Two Hetero Rings Bridged Or Fused Among Themselves Or Bridged Or Fused With A Common Carbocyclic Ring System, (e.g., Rifamycin, Etc.) Patents (Class 435/119)
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Publication number: 20130023017Abstract: There is provided a method for culturing a microorganism in which a particular polynucleotide or a recombinant vector comprising it/them is introduced with an intermediate compound necessary for biosynthesis of pyripyropene. A. The method of the present invention allows for the production of pyripyropene.Type: ApplicationFiled: January 19, 2011Publication date: January 24, 2013Inventors: Hiroyuki Anzai, Kentaro Yamamoto, Kazuhiko Oyama, Mariko Tsuchida, Kimihiko Goto, Masaaki Mitomi
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Publication number: 20130017581Abstract: There is provided a nucleic acid construct comprising a pyripyropene biosynthetic gene cluster and a marker gene. The nucleic acid construct according to the present invention provides an inexpensive and highly productive method for producing pyripyropene.Type: ApplicationFiled: January 19, 2011Publication date: January 17, 2013Inventors: Sato Aihara, Naomi Sumida, Koichiro Murashima, Kouji Yanai, Hiroyuki Anzai, Kentaro Yamamoto
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Publication number: 20130017580Abstract: The present disclosure relates to non-naturally occurring monooxygenase polypeptides useful for preparing prazole compounds, polynucleotides encoding the polypeptides, and methods of using the polypeptides.Type: ApplicationFiled: December 8, 2010Publication date: January 17, 2013Applicant: CODEXIS, INC.Inventors: Yong Koy Bong, Michael D. Clay, Steven J. Collier, Benjamin Mijts, Michael Vogel, Xiyun Zhang, Jun Zhu, Jovana Nazor, Derek Smith, Shiwei Song
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Publication number: 20130012527Abstract: The preQ1 riboswitch is a target for antibiotics and other small molecule therapies. The preQ1 riboswitch and portions thereof can be used to regulate the expression or function of RNA molecules and other elements and molecules. The preQ1 riboswitch and portions thereof can be used in a variety of other methods to, for example, identify or detect compounds. Compounds can be used to stimulate, active, inhibit and/or inactivate the preQ1 riboswitch. The preQ1 riboswitch and portions thereof, both alone and in combination with other nucleic acids, can be used in a variety of constructs and RNA molecules and can be encoded by nucleic acids.Type: ApplicationFiled: September 14, 2012Publication date: January 10, 2013Applicant: YALE UNIVERSITYInventors: Ronald R. Breaker, Jeffrey E. Barrick, Adam Roth, Wade Winkler
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Publication number: 20130004993Abstract: Methods for producing and obtaining natural products from microbial amplification chambers are described. This approach utilizes the concept of green chemistry to synthesize and extract the marine and terrestrial natural products. The method describes techniques to colonize and grow the selected bacteria and to continuously harvest the pharmaceutical agent from the broth without using any commercial solvents.Type: ApplicationFiled: June 28, 2011Publication date: January 3, 2013Applicants: University System of Georgia, Valdosta State UniversityInventor: Thomas J. Manning
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Publication number: 20120329108Abstract: The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.Type: ApplicationFiled: September 5, 2012Publication date: December 27, 2012Applicant: CODEXIS, INC.Inventors: Christopher Savile, Emily Mundorff, Jeffrey C. Moore, Paul N. Devine, Jacob M. Janey
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Publication number: 20120330022Abstract: There is provided a method for producing a pyripyropene derivative represented by the following formula A by an enzyme method. The production method of the present invention allows for production of a pyripyropene derivative under simpler conditions and in shorter steps. [wherein R represents a linear, branched or cyclic C2-6 alkylcarbonyl group (when the alkyl moiety of this group is branched or cyclic, C3-6 alkyl carbonyl group)].Type: ApplicationFiled: January 19, 2011Publication date: December 27, 2012Inventors: Kentaro Yamamoto, Mariko Tsuchida, Kazuhiko Oyama, Kimihiko Goto, Masaaki Mitomi
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Publication number: 20120315679Abstract: A process for the large-scale chemoenzymatic production of (6S)-5-methyl-5,6,7,8-tetrahydrofolic acid, also known as (6S)-5-methylTHFA, the process comprising the steps of: (1) reducing folic acid (FA) so as to yield dihydrofolic acid (DHFA); (2) stereoselectively reducing DHFA with dihydrofolate reductase (DHFR) in the presence of NADP/NADPH, glucose and GluDH so as to yield (6S)-THFA; (3) converting (6S)-THFA to (6S)-5-methlTHFA; and (4) isolating (6S)-5-methylTHFA,Type: ApplicationFiled: June 14, 2012Publication date: December 13, 2012Applicant: CHEMIC LABORATORIES INC.Inventors: Gerald S. Jones, JR., Joseph P. St. Laurent, Scott A. Goodrich, George Maguire
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Publication number: 20120302754Abstract: The present invention relates to the production of alkaloids from Liliaceae cell culture.Type: ApplicationFiled: June 18, 2010Publication date: November 29, 2012Applicant: PHYTON HOLDINGS, LLCInventors: Venkatesh Srinivasan, Jennifer Alford, Beth Slusar-Place, Charles Swindell, Michael Horn, Khisal Alvi, Gilbert Gorr
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Publication number: 20120297507Abstract: A method for producing leafy biomass from undifferentiated plant cells, the method comprising providing undifferentiated plant cells, contacting them with an agent that promotes differentiation of the cells into leafy tissue and growing the cells in a temporary liquid immersion culture system. This method of the invention may be used to produce polypeptides, and natural medicinal products, and can be used to capture carbon dioxide. A method of producing a polypeptide in plant cells in vitro comprising: providing undifferentiated plant cells containing chloroplasts that carry a transgenic nucleic acid molecule encoding the polypeptide, wherein the plant cells display homoplastomy; and propagating the cells according to the above method to produce leafy biomass containing the polypeptide.Type: ApplicationFiled: August 12, 2010Publication date: November 22, 2012Applicant: Imperial Innovations LimitedInventors: Franck Michoux, Peter Nixon, James Gerard McCarthy
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Publication number: 20120289714Abstract: The present invention relates to a method for preparing coumestrol, comprising a step in which beans are germinated and a step in which the beans are fermented, and to coumestrol prepared by the method.Type: ApplicationFiled: January 31, 2011Publication date: November 15, 2012Inventors: Hee Young Jeon, Dae Bang Seo, Si Young Cho, Sang Jun Lee
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Publication number: 20120288891Abstract: The present disclosure relates to the use of pantothenate compounds as a non-genetic switch for the production of heterologous acetyl-CoA derived (HACD) compounds in microbial host cells. The invention provides genetically modified microorganisms that are more stable when stored and initially cultured under reduced pantothenate concentrations, cell culture media having reduced concentrations of pantothenate compounds, and methods of producing HACD compounds using the cell culture media and the genetically engineered microorganisms of the invention.Type: ApplicationFiled: May 9, 2012Publication date: November 15, 2012Applicant: AMYRIS, INC.Inventor: Adam Meadows
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Publication number: 20120288905Abstract: The present invention provides isolated nucleic acids comprising nucleotide sequences encoding isoprenoid modifying enzymes, as well as recombinant vectors comprising the nucleic acids. The present invention further provides genetically modified host cells comprising a subject nucleic acid or recombinant vector. The present invention further provides a transgenic plant comprising a subject nucleic acid. The present invention further provides methods of producing an isoprenoid compound, the method generally involving culturing a subject genetically modified host cell under conditions that permit synthesis of an isoprenoid compound modifying enzyme encoded by a subject nucleic acid.Type: ApplicationFiled: March 21, 2012Publication date: November 15, 2012Inventors: Dae-Kyun Ro, Karyn Newman, Eric M. Paradise, Jay D. Keasling, Mario Ouellet, Rachel a. Krupa, Kimberly Ho, Timothy Ham
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Publication number: 20120276587Abstract: The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms by engineering a microorganism for increased carbon flux towards mevalonate production in the following enzymatic pathways: (a) citrate synthase, (b) phosphotransacetylase, (c) acetate kinase, (d) lactate dehydrogenase, (e) malic enzyme, and (f) pyruvate dehydrogenase such that one of more of the enzyme activity is modulated. In addition, production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids can be further enhanced by the heterologous expression of the mvaE and mvaS genes (such as, but not limited to, mvaE and mvaS genes from the organisms Listeria grayi DSM 20601, Enterococcus faecium, Enterococcus gallinarum EG2, and Enterococcus casseliflavus).Type: ApplicationFiled: April 27, 2012Publication date: November 1, 2012Applicant: Danisco US Inc.Inventors: Zachary Q. Beck, Michael C. Miller, Caroline M. Peres, Yuliya A. Primak, Jeff P. Pucci, Derek H. Wells
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Publication number: 20120270282Abstract: The present disclosure relates to engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.Type: ApplicationFiled: July 6, 2012Publication date: October 25, 2012Applicant: Codexis, Inc.Inventors: Behnaz Behrouzian, Anke Krebber, Emily Mundorff
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Patent number: 8293507Abstract: The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.Type: GrantFiled: February 26, 2010Date of Patent: October 23, 2012Assignee: Codexis, Inc.Inventors: Christopher Savile, Emily Mundorff, Jeffrey C. Moore, Paul N. Devine, Jacob M. Janey
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Patent number: 8263369Abstract: A compound having a structure expressed by the following Structural Formula (1):Type: GrantFiled: March 1, 2011Date of Patent: September 11, 2012Assignee: Microbial Chemistry Research FoundationInventors: Hiroyuki Inoue, Manabu Kawada, Daishiro Ikeda
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Publication number: 20120220789Abstract: A novel substance exhibiting physiologically active effects is produced from a tea leaf. A novel polyphenol derivative is produced, for example, by subjecting a microbially fermented tea leaf obtained by culturing Aspergillus sp. (PK-1) with a tea leaf, to an extraction treatment.Type: ApplicationFiled: September 17, 2010Publication date: August 30, 2012Applicants: SAGA UNIVERSITY, KABUSHIKI KAISHA RIVERSONInventors: Teruyoshi Yanagita, Kanji Ishimaru, Takashi Tanaka, Kazunori Koba, Hitoshi Miyazaki, Naohito Aoki, Denbei Kawamura
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Publication number: 20120190651Abstract: This invention relates to novel compounds obtained by fermentation of Myxobacteria strain (PM0670013/MTCC 5570). The present invention further relates to the processes for the production of the novel anti-inflammatory compounds, to the culture no. PM0670013 (MTCC 5570), and to pharmaceutical compositions containing compounds of the present invention as an active ingredient and its use in medicines for the treatment of inflammatory diseases or disorders mediated by proinflammatory cytokines such as Tumor Necrosis Factor-alpha (TNF-?) and/or interleukins such as IL-6, having anti-inflammatory activity The invention also includes all stereoisomeric forms of compounds of the present invention.Type: ApplicationFiled: January 24, 2011Publication date: July 26, 2012Inventors: Koteppa Pari, Girish Badrinath Mahajan, Nidhi Tomar, Vijaya Phani Kumar Yemparala, Asha Adrian Kulkarni-Almeida, Saji George
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Patent number: 8187844Abstract: Natamycin is separated and recovered from a fermentation broth containing biomass and natamycin by adjusting the pH of a natamycin-containing suspension first to dissolve the natamycin in a solvent then adjusting the pH again to precipitate the dissolved natamycin which is used as an antifungal used to protect products susceptible to fungal spoilage.Type: GrantFiled: September 29, 2006Date of Patent: May 29, 2012Assignee: DSM IP Assets B.V.Inventors: Ferdinand Theodorus Jozef Van Rijn, Willem Johannes Wildeboer
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Publication number: 20120115897Abstract: The present invention designs and synthesizes the ascorbyl ester derivatives of the aryl (ethanoic) propanoic acid non-steroidal anti-inflammatory medicaments, such as ibuprofen, ketoprofen and naproxen, and addition salt of the derivatives with pharmaceutical acid or pharmaceutical alkaline. The non-steroidal anti-inflammatory medicament which takes the ibuprofen as the representative is a common antipyretic analgesic medicament. The invention has remarkable antipyretic and analgesic effects and good safety except for anti-inflammatory effect, thus being not only suitable for adults, but also suitable for the elderly people, infants and children.Type: ApplicationFiled: December 24, 2009Publication date: May 10, 2012Applicants: WUXI HONGRUI BIO-PHARMA-TECH CO., LTD .Inventors: Luhong Tang, Ajuan Dai, Ze Wang, Yang Sun, Xinning Liu, Lingyan Xu, Xin Fang, Shuang Qiu, Yaqing Cao, Xiaomin Xu, Ruixia Jiang, Chao Wu
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Publication number: 20120108494Abstract: The present invention provides compositions comprising amiloride amino acid and peptide conjugates. Efficient methods are also provided for administering the compositions for treating cancer and for delivering an amiloride conjugate into cancer cells in a subject in need thereof.Type: ApplicationFiled: August 1, 2008Publication date: May 3, 2012Applicant: The Regents of the University of CaliforniaInventors: Fredric A. Gorin, Michael H. Nantz
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Publication number: 20120100579Abstract: A triprenyl phenol compound represented by the following formula (II) and (III) and having a thrombolysis-enhancing activity, and an efficient method for producing the triprenyl phenol compound. In formula (II) and (III), R1 represents an aromatic group having as a substituent or as a part of a substituent at least one member selected from the group consisting of a carboxyl group, a hydroxyl group, a sulfonic acid group and a secondary amino group, or an aromatic group which comprises a secondary amino group and may comprise nitrogen; R4 in the general formula (III) represents an aromatic amino acid residue represented by the general formula (III-1) below; X represents —CHY—(CH3)2Z; and Y and Z are respectively —H or —OH or together form a single bond: (III-1) wherein R5 represents a hydroxyl group which may be present or absent; and n represents an integer of 0 or 1.Type: ApplicationFiled: January 4, 2012Publication date: April 26, 2012Applicant: TOKYO UNIVERSITY OF AGRICLATURE AND TECHNOLOGY TLO CO., LTD.Inventors: Keiji HASUMI, Yoshikazu KITANO, Hideo OHISHI, Haruki KOIDE, Keiko HASEGAWA, Ritsuko NARASAKI
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Publication number: 20120083607Abstract: Provided are processes for the synthesis of amino analogues from ketone starting materials.Type: ApplicationFiled: July 1, 2011Publication date: April 5, 2012Applicant: Infinity Pharmaceuticals, Inc.Inventors: Brian c. Austad, Adilah Bahadoor, Jitendra D. Belani, Somarajannair Janardanannair, Charles W. Johannes, Gregg F. Keaney, Priscilla L. White, Sheldon L. Wallerstein
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Publication number: 20120083527Abstract: The present disclosure relates generally to compositions and methods for treating cancer. In some aspects, novel spirohexenolides and methods of using and producing them are described.Type: ApplicationFiled: April 16, 2010Publication date: April 5, 2012Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Michael D. Burkart, James J. La Clair, Minjin Kang, Brian D. Jones, Alexander L. Mandel, Wei-Luen Yu, Justin C. Hammons
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Publication number: 20120070866Abstract: Food products fortified by purified PQQ and methods of application and production are disclosed. A method of preserving a food product comprising admixing an effective amount of purified PQQ or a compound containing purified PQQ derivatives with a foodstuff to provide an inhibiting means to microorganism growth in the food product.Type: ApplicationFiled: November 30, 2011Publication date: March 22, 2012Applicant: Shanghai Medical Life Science Research Center, LtdInventors: Jianxin Gu, Xiaojing Yun, Xiaoning Chen, Si Zhang, Junwu Yang, Yihong Wu
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Publication number: 20120065359Abstract: An enzymatic polymerization method of covalently linking a colorant to a polyester to produce a colorant-polyester compound, the method including the steps of: (a) providing a reaction solution comprised of an ester monomer, a colorant having or functionalized to have a hydroxyl group, and an enzymatic catalyst; (b) reacting the ester monomer and the colorant using the enzymatic catalyst to produce a polymeric product, wherein the polymeric product includes a colorant-polyester compound; and (c) separating the polymeric product from the reaction solution. A colorant-polyester compound includes a colorant covalently linked to a polyester and the polyester is obtained by polymerizing a lactone.Type: ApplicationFiled: September 10, 2010Publication date: March 15, 2012Applicant: XEROX CORPORATIONInventors: Santiago FAUCHER, Kimberly D. Nosella, Rosa M. Duque
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Publication number: 20120053344Abstract: Methods for producing phycotoxins from natural sources, wherein the phycotoxins have a definite compositional profile are described herein. In one embodiment, the phycotoxins are produced by cyanobacteria. In one embodiment, the phycotoxins are produced by continuously culturing cyanobacteria under strictly controlled conditions in order to produce a definite compositional profile. In another embodiment, organic nutrients are added to the culture that allows for higher concentrations of neosaxitoxin and saxitoxin or gonyaulatoxins 2 and 3 per weight of the algae. The phycotoxins are isolated primarily from the bacteria but can also be isolated from the culture medium. In one embodiment, the cyanobacteria produce only neosaxitoxin and saxitoxin in a ratio of about 6:1, 5:1, 4:1, or 3:1. In a preferred embodiment, the amount of saxitoxin is less than 20% by weight of the total amount of neosaxitoxin and saxitoxin produced. In another embodiment, the cyanobacteria produce only GTX2 and GTX 3.Type: ApplicationFiled: September 23, 2011Publication date: March 1, 2012Inventor: Marcelo Santiago Lagos González
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Publication number: 20120041216Abstract: The invention relates to a method for the amination of at least one keto group in a multicyclic ring system comprising at least one keto group into an amino group, using at least one enzyme E having transaminase activity.Type: ApplicationFiled: January 13, 2010Publication date: February 16, 2012Applicant: EVONIK DEGUSSA GmbHInventors: Volker Sieber, Katrin Grammann, Broder Ruehmann, Thomas Haas, Jan Christoph Pfeffer, Kai Doderer, Claudia Rollmann, Arne Skerra, Christian Rausch, Alexandra Lerchner
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Publication number: 20120015410Abstract: An isolated novel polynucleotide comprising a nucleotide sequence encoding at least one polypeptide involved in biosynthesis of pyripyropene A, a recombinant vector comprising the polynucleotide and a transformant comprising the polynucleotide are disclosed. By the present invention, a pyripyropene A biosynthetic gene useful for production of a novel pyripyropene analog, improvement of productivity of a pyripyropene A-producing bacterium, production of an insecticidal agent for microorganisms, creation of a plant resistant to insect pests or the like are provided.Type: ApplicationFiled: July 24, 2009Publication date: January 19, 2012Inventors: Hiroyuki Anzai, Kentaro Yamamoto, Mariko Tsuchida, Kazuhiko Oyama, Masaaki Mitomi
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Publication number: 20110318790Abstract: The present invention relates to eukaryotic cells which have the ability to isomerise xylose directly into xylulose. The cells have acquired this ability by transformation with nucleotide sequences coding for a xylose isomerase that has one or more specific sequence elements typical for isomerases having the ability of functional expression in yeasts, such as e.g. xylose isomerases obtainable from a bacterium of the genera Clostridiumand Fusobacteriumor a tunicate form the genus Ciona. The cell preferably is a yeast or a filamentous fungus, more preferably a yeast is capable of anaerobic alcoholic fermentation.Type: ApplicationFiled: December 24, 2009Publication date: December 29, 2011Inventors: Aloysius Wilhelmus Rudolphus Hubertus Teunissen, Johannes Adrianus Maria De Bont
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Publication number: 20110312034Abstract: The invention relates to novel molecules and libraries thereof as well as methods for their production. Methods of producing the novel molecules include the cleaving of starting molecules into molecular subunits and the assembly of the subunits into novel recombined molecules.Type: ApplicationFiled: March 2, 2011Publication date: December 22, 2011Inventor: Dennis M. Brown
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Patent number: 8067213Abstract: There is disclosed an improved poppy straw of a stably reproducing Papaver somniferum for the extraction of thebaine and/or oripavine, the threshed straw having thebaine and oripavine constituting about 50% by weight or greater of the alkaloid combination consisting of morphine, codeine, thebaine and oripavine.Type: GrantFiled: February 26, 2004Date of Patent: November 29, 2011Assignee: Tasmanian Alkaloids Pty., Ltd.Inventors: Anthony John Fist, Christopher James Byrne, Wayne Lyle Gerlach
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Patent number: 8053217Abstract: Method to produce eritadenine by liquid phase fermentation of Lentinus edodes without formation of the fruit body wherein the Lentinus edodes is exposed to shear during its cultivation.Type: GrantFiled: May 6, 2008Date of Patent: November 8, 2011Assignee: Working Bugs ABInventors: Kris Arvid Berglund, Ulrika Rova, Josefine Enman
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Publication number: 20110250656Abstract: The present invention relates to the field of biotechnological production of polyketide based colorants from filamentous fungi, in particular a method for preparing a biomass comprising a Monascus-like pigment composition from a nontoxigenic and non-pathogenic fungal source. The present invention further relates to use of the Monascus-like pigment composition as a colouring agent for food items and/or non-food items, and a cosmetic composition comprising the Monascus-like pigment composition.Type: ApplicationFiled: August 28, 2008Publication date: October 13, 2011Applicant: DTU, TECHNICAL UNIVERSITY OF DENMARKInventors: Sameer A.S. Mapari, Anne S. Meyer, Jens C. Frisvad, Ulf Thrane
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Publication number: 20110237533Abstract: Compounds represented by formula (I) wherein the symbols are as defined in the description, exhibit a lipase inhibitory activity, and are useful for foods, drinks, and the like.Type: ApplicationFiled: April 1, 2011Publication date: September 29, 2011Applicant: AJINOMOTO CO.,INC.Inventors: Kenzo NOMURA, Yayoi Kanbayashi
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Publication number: 20110213142Abstract: The present invention relates to a method for biologically producing heme-iron through microorganism cultivation, and to an iron supplementing composition containing the heme-iron produced by the same. The present invention provides a method for biologically producing heme-iron through microorganism cultivation, making it possible to economically produce heme-iron or heme-iron preparations which can be safely used as an iron supplement.Type: ApplicationFiled: September 2, 2009Publication date: September 1, 2011Applicant: Catholic University Industry Academic Cooperation FoundationInventor: Pil Kim
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Publication number: 20110213164Abstract: A compound having a structure expressed by the following Structural Formula (1):Type: ApplicationFiled: March 1, 2011Publication date: September 1, 2011Applicant: MICROBIAL CHEMISTRY RESEARCH FOUNDATIONInventors: Hiroyuki Inoue, Manabu Kawada, Daishiro Ikeda
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Patent number: 8003358Abstract: The present invention discloses a two-step enzyme method for preparing 7-aminocephalosporanic acid from cephalosporin C, wherein D-amino acid oxidase used is purified D-amino acid oxidase mutant of yeast Trigonopsis variabilis, having a specific activity of 105% higher than that of parent D-amino acid oxidase. The method has no need of addition of hydrogen peroxide, ?-lactamase inhibitor, catalase inhibitor, catalase and the like commonly used in the prior art. The productivity of the method can reach more than 93%. Thus, the method is simple, low in cost and high in productivity.Type: GrantFiled: August 2, 2006Date of Patent: August 23, 2011Assignee: Bioright Worldwide Company LimitedInventors: Jun Wang, Waikei Tsang, Hongkin Yap, Junmin Chen, Yaulung Siu, Supyin Tsang
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Publication number: 20110183385Abstract: The present invention relates to a method of fermenting and purifying tricyclo compounds, specifically FK506 and/or FK520, and more particularly, to a method of purifying tricyclo compounds by adding a hydrophobic absorbent resin as a carrier in culturing FK506 and/or FK520 producing bacteria.Type: ApplicationFiled: May 6, 2008Publication date: July 28, 2011Inventors: Jae Jong Kim, Si Kyu Lim, Mi Ok Lee, Sang Myoun Lim, Bo-mi Lee, Dong Hwan Kim, Jeoung Hyun, Keum Soon Lee
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Patent number: 7977074Abstract: The present invention relates methods for the biosynthesis of tetrodotoxin (TTX) involving the steps of obtaining a culture possessing one or more of a Vibrio species, such as through a seed culture, inoculating the culture and tissue extract from a textrodotoxin-bearing organism in a fermenter medium, and isolating and purifying tetrodotoxin from said fermenter, resulting in a yield of TTX of about 0.5 g TTX/L in about 3 to 5 days, such TTX being at least 90% pure.Type: GrantFiled: February 23, 2007Date of Patent: July 12, 2011Assignee: The Hong Kong Polytechnic UniversityInventors: Peter Hoi-fu Yu, Chun Fai Yu
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Publication number: 20110165633Abstract: The present invention relates to production of polyketides and other natural products and to libraries of compounds and individual novel compounds. One important area is the isolation and potential use of novel FKBP-ligand analogues and host cells that produce these compounds. The invention is particularly concerned with methods for the efficient transformation of strains that produce FKBP analogues and recombinant cells in which cloned genes or gene cassettes are expressed to generate novel compounds such as polyketide (especially rapamycin) FKBP-ligand analogues, and to processes for their preparation, and to means employed therein (e.g. nucleic acids, vectors, gene cassettes and genetically modified strains).Type: ApplicationFiled: March 11, 2011Publication date: July 7, 2011Inventors: Matthew Alan Gregory, Sabine Gaisser, Hrvoje Petkovic, Steven Moss
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Patent number: 7972836Abstract: The present invention relates to a method for preparing an optically active 4-hydroxy-1,2,3,4-tetrahydroquinoline compound [I], which comprises the steps of: treating a racemic 4-hydroxy-1,2,3,4-tetrahydroquinoline compound represented by general formula [I]: [wherein R1 represents a hydrogen atom or a protecting group for amino group.] with an enzyme having an ability of selectively or preferentially acylating one enantiomer of the racemic compound [I] in the presence of an acyl donor; and if necessary, subjecting the reaction product to solvolysis.Type: GrantFiled: October 4, 2006Date of Patent: July 5, 2011Assignee: Mitsubishi Tanabe Pharma CorporationInventors: Masaki Okamoto, Akira Sakuragi, Muneki Kishida, Yoshikazu Mori
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Publication number: 20110144176Abstract: This invention relates, in part, to macrolide compounds, actinomycete strains for producing them, and pharmaceutical compositions containing them.Type: ApplicationFiled: December 15, 2010Publication date: June 16, 2011Applicant: Wyeth LLCInventors: Mia Haruna Yoshino SUMMERS, Edmund Idris Graziani, Margaret M. Leighton, Kevin Pong, Roger Alan Kele, David P. Labeda
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Publication number: 20110136753Abstract: Glycosylated indolocarbazoles, method for obtaining same and uses thereof. This invention relates to rebeccamycin and staurosporine derivatives obtained by fermentation of recombinant bacterial strains. The invention also relates to the methods used to obtain the recombinant strains and the production of rebeccamycin and staurosporine derivatives. The invention also relates to bacterial strains that are of use for the production of rebeccamycin and staurosporine derivatives. Lastly, the rebeccamycin and staurosporine derivatives described herein are applicable to the field of human health, specifically for manufacturing drugs that are of use in the treatment of tumour, neurological and inflammatory diseases.Type: ApplicationFiled: April 6, 2009Publication date: June 9, 2011Applicants: UNIVERSIDAD DE OVIEDO, ENTRECHEM S.L.Inventors: Aaroa Pêrez Salas, César Sánchez Reillo, Alfredo Fernández Braña, Carmen Méndez Fernández, Jose Antonio Salas Fernández, Francisco Morís Varas
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Publication number: 20110137036Abstract: The present invention relates to the stereoselective synthesis of (4aS,7aS)-octahydro-1H-pyrrolo[3,4-b]pyridine, as well as the conversion thereof, to give Moxifloxacin. Particularly, the present invention relates to a method for the synthesis of (4aS,7aS)-octahydro-1H-pyrrolo[3,4-b]pyridine of formula (I) comprising: (a) the optical resolution by enzymatic hydrolysis of the intermediate dialkyl-1-alkylcarbonylpiperidine-2,3-dicarboxylate racemate of formula (II) to give, following isolation, the intermediate dialkyl-(2S,3R)-1-alkylcarbonyl-piperidine-2,3-dicarboxylate of formula (III) in which AIk is a straight or branched C1-C5 alkyl group; (b) the conversion of the intermediate (III) to (4aR,7aS)-1-alkylcarbonylhexahydrofuro[3,4-b]pyridine-5,7-dione of formula (IV) in which AIk has the meanings set forth above; (c) the conversion of the intermediate (IV) to (4aS,7as)-octahydro-1H-pyrrolo[3,4-b]pyridine of formula (I) with an optical purity above 99%.Type: ApplicationFiled: March 4, 2010Publication date: June 9, 2011Inventors: Riccardo Motterle, Giancarlo Arvotti, Elisabetta Bergantino, Andrea Castellin, Stefano Fogal, Marco Galvagni
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Patent number: 7939060Abstract: This invention provides a composition comprising a daidzein-containing substrate and a strain of micro-organism capable of metabolizing daidzein to equol as essential ingredients. This composition is effective in the prevention and alleviation of unidentified clinical syndrome inclusive of menopausal syndrome in middle-aged to elderly women for which no effective means of prevention or alleviation has heretofore been available.Type: GrantFiled: January 9, 2008Date of Patent: May 10, 2011Assignee: Otsuka Pharmaceutical Co., Ltd.Inventors: Shigeto Uchiyama, Tomomi Ueno, Megumi Kumemura, Kiyoko Imaizumi, Kyosuke Masaki, Seiichi Shimizu
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Publication number: 20110104360Abstract: The invention provides an enzyme having a lignan-hydroxylating activity, particularly an enzyme capable of catalyzing the reaction of transferring a hydroxyl group to a lignan, an enzyme capable of catalyzing the hydroxylation of piperitol to 9-hydroxylpiperitol or pinoresinol to 9-hydroxylpinoresinol. The invention provides a polypeptide having a lignan-hydroxylating activity; a polynucleotide encoding the same; a vector or transformant comprising the polynucleotide; a method for producing a polypeptide having a lignan-hydroxylating activity which comprises using the transformant; and so on. The transformant wherein the polynucleotide is expressibly introduced is useful for the hydroxylation of a lignan or for the production of a product using the same in the food sector and a variety of industry sectors.Type: ApplicationFiled: December 17, 2008Publication date: May 5, 2011Applicant: SUNTORY HOLDINGS LIMITEDInventors: Eiichiro Ono, Asako Okada, Yuko Fukui
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Publication number: 20110092534Abstract: A compound having the general the general structure I is provided: (Formula I), wherein Z is a substituent selected from a group consisting of S and O; X is a substituent selected from a group consisting of Cl, Br, I, OH and NH2; each of R1 and R2 is a substituent independently selected from a group consisting of H, a linear, cyclic or branched, substituted or unsubstituted C1-C6 alkyl, a substituted or unsubstituted aryl, and a substituted or unsubstituted heteroaryl; and each of Y and Y1 is a substituent independently selected from a group consisting of H and C(O)—C6H4—Br.Type: ApplicationFiled: June 27, 2008Publication date: April 21, 2011Inventors: William Fenical, Paul Jensen, John Macmillan, Chambers C. Hughes, James J. Laclair
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Publication number: 20110091943Abstract: The present invention relates to an semi-continuous or continuous process for the regioselective enzymatic hydrolysis of alcohol groups protected e.g. as esters or amino-acid esters or phosphate groups The process of the present invention is useful for instance for the selective enzymatic hydrolysis of pyranosides or furanosides having more than one hydrolysable groups.Type: ApplicationFiled: June 25, 2008Publication date: April 21, 2011Applicant: Andadys Pharmaceuticals, Inc.Inventors: Fabrice Gallou, Pascal Beney