Fat; Fatty Oil; Ester-type Wax; Higher Fatty Acid (i.e., Having At Least Seven Carbon Atoms In An Unbroken Chain Bound To A Carboxyl Group); Oxidized Oil Or Fat Patents (Class 435/134)
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Publication number: 20130197248Abstract: The present invention relates to the provision of genetically modified fungal cells, such as yeast cells with an improved ability for producing different fatty acids and specifically fatty acid ethyl esters (FAEE), the main components of biodiesel. An increased in fatty acid production, and hence in FAEE, is obtained in the first place by expressing different heterologous polypeptides in combination with the down-regulation, attenuation, deletion or over-expression of specially selected genes, wherein said genes encode enzymes involved in the fatty acids synthesizing pathway, fatty acid consuming pathways, carbohydrate biosynthesis pathways or enzyme acting as wax ester transporters or a combination thereof. The methods and products of the invention would allow large-scale production of FAEE with carbohydrates as the only externally-supplied substrate.Type: ApplicationFiled: August 6, 2011Publication date: August 1, 2013Inventors: Jens Nielsen, Shuobo Shi
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Publication number: 20130197086Abstract: This invention refers to a procedure for obtaining fatty acids of pharmacological and nutritional interest that comprises the steps of feeding a gas comprising CO2 into a reactor that contains a culture that comprises at least one species of microalgae capable of photosynthesis, the process of photosynthesis by the species of microalgae from the CO2 supplied, producing a biomass that contains a general formula (I) compound: extraction of the general formula (I) compound from the biomass obtained and concentration and/or purification of this compound.Type: ApplicationFiled: June 23, 2010Publication date: August 1, 2013Inventor: Bernard A. J. Stroïazzo Mougin
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Patent number: 8497116Abstract: The invention provides methods of manufacturing oils and oil-based products such as transportation fuels, industrial chemicals, lubricants and plastics using sugar cane, sugar cane juice, corn steep liquor, corn starch or depolymerized cellulosic material as a feedstock for bioproduction processes. Oils are manufactured using microalgae expressing sucrose invertase, which allows the microalgae to produce oil using sucrose-based feedstocks, along or in combination with other feedstocks.Type: GrantFiled: December 3, 2010Date of Patent: July 30, 2013Assignee: Solazyme, Inc.Inventors: Donald E. Trimbur, Chung-Soon Im, Harrison F. Dillon, Anthony G. Day, Scott Franklin, Anna Coragliotti
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Patent number: 8497099Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.Type: GrantFiled: February 7, 2013Date of Patent: July 30, 2013Assignee: DSM IP Assets B.V.Inventors: Paul W. Behrens, John M. Thompson, Kirk Apt, Joseph W. Pfeifer, III, James P. Wynn, James Casey Lippmeier, Jaouad Fichtali, Jon Hansen
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Patent number: 8497100Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.Type: GrantFiled: February 7, 2013Date of Patent: July 30, 2013Assignee: DSM IP Assets B.V.Inventors: Paul W. Behrens, John M. Thompson, Kirk Apt, Joseph W. Pfeifer, III, James P. Wynn, James Casey Lippmeier, Jaouad Fichtali, Jon Hansen
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Publication number: 20130189749Abstract: The present invention provides compositions, methods, and kits comprising PUFAs produced by microalgae, in particular omega-3 and/or omega-6 fatty acids produced by members of the genus Rhodomonas, in particular Rhodomonas salina. The invention also provides compositions, methods, and kits comprising the PUFAs for the prophylactic and/or therapeutic treatment of a disease or condition, in particular a cardiovascular and/or inflammatory disease or condition.Type: ApplicationFiled: December 28, 2012Publication date: July 25, 2013Applicant: WAKE FOREST UNIVERSITY SCHOOL OF MEDICINEInventor: Wake Forest University School of Medicine
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Publication number: 20130190521Abstract: The disclosed subject matter relates generally to a method for modifying oil, and specifically to a process for increasing the concentration of polyunsaturated fatty acid in an oil composition.Type: ApplicationFiled: March 8, 2013Publication date: July 25, 2013Applicant: OCEAN NUTRITION CANADA LIMITEDInventor: Ocean Nutrition Canada Limited
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Patent number: 8486672Abstract: To provide a method for supplying lauric acid with algae. The method for producing an oil or fat containing lauric acid as a constituent fatty acid includes culturing algae in the class Chlorarachniophyceae in a medium and recovering, from the culture product, an oil or fat having a lauric acid content of 3 weight % or higher of the fatty acid composition.Type: GrantFiled: March 24, 2011Date of Patent: July 16, 2013Assignee: Kao CorporationInventors: Hiroshi Yoshida, Fumikazu Takahashi, Yasushi Takimura
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Patent number: 8486671Abstract: The present invention relates to a process for producing polyunsaturated fatty acids in an organism by introducing nucleic acids into the organism which code for polypeptides having acyl-CoA:lysophospholipid a cyltransferase activity. Advantageously, these nucleic acid sequences may, if appropriate together with further nucleic acid sequences coding for biosynthesis polypeptides of the fatty acid or lipid metabolism, be expressed in the transgenic organism. The invention furthermore relates to the nucleic acid sequences, to nucleic acid constructs comprising the nucleic acid sequences of the invention, to vectors comprising the nucleic acid sequences and/or the nucleic acid constructs and to transgenic organisms comprising the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced by the process of the invention and to their use.Type: GrantFiled: April 2, 2009Date of Patent: July 16, 2013Assignee: BASF Plant Science GmbHInventors: Andreas Renz, Ernst Heinz, Amine Abbadi, Frederic Domergue, Thorsten Zank
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Patent number: 8480904Abstract: A process for purifying a lipid composition having predominantly neutral lipid components having at least one long chain polyunsaturated fatty acid is disclosed. The process employs contacting the lipid composition with a polar solvent, such as acetone, wherein the solvent is selected such that contaminants are less soluble in the solvent than is the long chain polyunsaturated fatty acid. The process is typically conducted at cooler temperatures, including about 0° C. Upon precipitation of the contaminants from the lipid composition, a separation is conducted to remove the precipitated material from the lipid composition. The long chain polyunsaturated fatty acids can include ARA, DPA, EPA, and/or DHA. The process of the present invention effectively winterizes lipid compositions, thereby reducing the tendency of such compositions to become hazy.Type: GrantFiled: September 6, 2011Date of Patent: July 9, 2013Assignee: DSM IP Assets B.V.Inventors: Daniel G. Dueppen, Samuel G. Zeller, Sandra I. Diltz, Robert H. Driver
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Publication number: 20130171702Abstract: A novel method for culturing mixotrophic single-cell algae makes it possible to enrich the lipid content of these algae, the enrichment being induced by a variable or discontinuous provision of light, in particular in the form of flashes.Type: ApplicationFiled: September 15, 2011Publication date: July 4, 2013Applicant: FERMENTALGInventor: Pierre Calleja
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Publication number: 20130172590Abstract: The present invention is directed to isolated microorganisms as well as strains and mutants thereof, biomasses, microbial oils, compositions, and cultures; methods of producing the microbial oils, biomasses, and mutants; and methods of using the isolated microorganisms, biomasses, and microbial oils.Type: ApplicationFiled: July 20, 2012Publication date: July 4, 2013Inventors: Joseph W. PFEIFER III, Jon Milton Hansen, Jose R. Garcia, Xiao Daniel Dong, Paul Warren Behrens, Kirk E. Apt
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Patent number: 8475543Abstract: A process for production of biofuels from algae can include cultivating an oil-producing algae by promoting sequential photoautotrophic and heterotrophic growth. The method can further include producing oil by heterotrophic growth of algae wherein the heterotrophic algae growth is achieved by introducing a sugar feed to the oil-producing algae. An algal oil can be extracted from the oil-producing algae, and can be converted to form biodiesel.Type: GrantFiled: February 14, 2011Date of Patent: July 2, 2013Assignee: Genifuel CorporationInventor: James R. Oyler
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Patent number: 8476059Abstract: The invention provides methods of manufacturing oils and oil-based products such as transportation fuels, industrial chemicals, edible oils, lubricants and plastics using sugar cane, sugar beets, and cane/beet agricultural processing byproducts as a feedstock for bioproduction processes. The disclosed processes utilize oil-bearing microbes as a conversion technology to convert chemical energy produced by sugar cane and sugar beets into energy-containing oils and oil derivatives. Also provided herein are oil-bearing microbes containing one or more exogenous sucrose utilization genes.Type: GrantFiled: June 2, 2008Date of Patent: July 2, 2013Assignee: Solazyme, Inc.Inventors: Donald E. Trimbur, Chung-Soon Im, Harrison F. Dillon, Anthony G. Day, Scott Franklin, Anna Coragliotti
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Patent number: 8476046Abstract: Disclosed is a method of pre-treating and saccharifying an algae biomass, by dehydrating the algae biomass to have a water content of about 10% to about 70% by weight, cutting the algae biomass having a water content of about 10% to about 70% by weight to a predetermined size, and saccharifying the cut algae biomass using a hydrolysis catalyst and/or a hydrolase to yield a monosaccharide.Type: GrantFiled: June 22, 2009Date of Patent: July 2, 2013Assignee: Samsung Electronics Co., Ltd.Inventors: Hwa Young Cho, Jun Seok Kim, Jae Chan Park, Sung Min Park, Jin Woo Kim
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Publication number: 20130164799Abstract: An energy efficient process for the preparation of marine microalgae Chlorella fatty acid methyl ester (CME) from hydrolysate of deoiled cake of Jatropha (JOCH) and crude glycerol co-product stream (GL7 and GL8) along with seawater diluted with tap water (1:2). A small part of the crude glycerol layer in case of JME is processed to recover glycerol for glycerol washing and the otherwise problematic still bottom is utilized for microbial synthesis of PHAs and the rest is utilized for Microalgal conversion of JME byproducts into CME. The remaining part of the methanol-depleted glycerol layer is utilized, along with hydrolysate of the Jatropha deoiled cake (JOCH), for single-stage Microalgal production of lipids by a marine Microalgal isolate (Chlorella sp.) without the need for any other nutrients. Waste streams from the microalgal processes can be discharged directly into agricultural fields as biofertilizer or recycled back in the mass cultivation.Type: ApplicationFiled: September 22, 2011Publication date: June 27, 2013Applicant: COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCHInventors: Pushpito Kumar Ghosh, Sandhya Chandrika Prasad Mishra, Mahesh Ramniklal Gandhi, Sumesh Chandra Upadhyay, Sanjiv Kumar Mishra, Imran Pancha, Anupama Vijaykumar Shrivastav, Deepti Jain, Bhumi Shethia, Subama Maiti, Krushnadevsinh Sukhdevsingh Zala
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Publication number: 20130160353Abstract: Disclosed herein are oils, including fatty acid triglyceride and fatty acid ester compositions suitable for the production of biofuel, biofuels, and methods for producing such materials and compositions. These materials and compositions comprise substantially no sterol glycosides.Type: ApplicationFiled: December 26, 2012Publication date: June 27, 2013Applicant: BP Corporation North America Inc.Inventor: BP Corporation North America Inc.
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PROCESS FOR THE IMPROVED SEPARATION OF A HYDROPHOBIC ORGANIC SOLUTION FROM AN AQUEOUS CULTURE MEDIUM
Publication number: 20130164797Abstract: A process for the improved separation of a hydrophobic organic solution from an aqueous culture medium is provided. The process includes preparing an aqueous culture medium of a metabolically active cell having a decreased activity; contacting of the aqueous culture medium with a hydrophobic organic solution comprising a substrate for biotransformation; conducting a biotransformation of the substrate; and separating the hydrophobic organic solution comprising a biotransformed substrate from the aqueous culture medium. The decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell and the decreased activity is of at least of one enzyme that catalyses one reaction of ?-oxidation of fatty acids.Type: ApplicationFiled: December 20, 2012Publication date: June 27, 2013Inventors: Jasmin GIELEN, Thomas Haas, Hans-Georg Hennemann, Steffen Schaffer, Mirja Wessel, Katharina Berse, Marion Schiebelhut, Sabine Hafkemeyer, Markus Poetter, Frank Erhardt -
Publication number: 20130164798Abstract: The present invention relates to processes for producing industrial products such as hydrocarbon products from non-polar lipids in a vegetative plant part. Preferred industrial products include alkyl esters which may be blended with petroleum based fuels.Type: ApplicationFiled: December 21, 2012Publication date: June 27, 2013Applicant: Commonwealth Scientific and Industrial Research OrganisationInventors: Thomas Vanhercke, James Robertson Petrie, Anna El Tahchy, Surinder Pal Singh, Qing Liu
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Publication number: 20130164795Abstract: The present invention includes methods of generating co-products for animal feed and compositions useful as co-products for animal feed derived from biofuel production processes. More specifically, the invention includes co-products for animal feed from at least one process feedstream, such as fatty acids from oil hydrolysis, lipids from evaporation of thin stillage, syrup, distillers grains, distillers grains and solubles, solids from a mash before fermentation, and solids from a whole stillage after fermentation.Type: ApplicationFiled: June 15, 2012Publication date: June 27, 2013Applicant: BUTAMAX(TM) ADVANCED BIOFUELS LLCInventors: David J. LOWE, Brian Michael Roesch
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Publication number: 20130164796Abstract: Systems and methods for the removal of contaminants from a liquid culture microalgae and/or cyanobacteria comprise an let tube, a pump, a gas injector, a vertical chamber, and/or a collection container that promotes the production of foam in the microalgae culture, wherein the foam contains the contaminants.Type: ApplicationFiled: June 27, 2012Publication date: June 27, 2013Inventors: Jason Licamele, Anna Lee Y. Tonkovich, Alexander Sitek, Scott Kuhlman
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Patent number: 8470571Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding a delta-8 desaturase along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using this delta-8 desaturase in plants and oleaginous yeast are disclosed.Type: GrantFiled: September 21, 2011Date of Patent: June 25, 2013Assignee: E. I. du Pont de Nemours and CompanyInventors: Howard Glenn Damude, Quinn Qun Zhu
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Publication number: 20130160169Abstract: The invention is directed to isolated polynucleotide and polypeptides of the CoD5, CoD6 and CoE6 genes from Conidiobolus obscurus, the CtE6 gene from Conidiobolus thromboids, and the PgDesX gene from Puccinia graminis; nucleic acid constructs, vectors and host cells incorporating the polynucleotide sequences; and methods of producing and using same.Type: ApplicationFiled: December 19, 2011Publication date: June 20, 2013Applicants: THE UNIVERSITY OF SASKATCHEWAN, THE GOVERNORS OF THE UNIVERSITY OF ALBERTAInventor: Xiao QIU
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Publication number: 20130158280Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.Type: ApplicationFiled: February 7, 2013Publication date: June 20, 2013Applicant: DSM IP Assets B.V.Inventor: DSM IP Assets B.V.
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Publication number: 20130157325Abstract: Molecular dynamics (MD) simulation on the three-dimensional structure of Candida anrtarctica lipase B revealed two hitherto unknown lids with a marked mobility, and this discovery was used to design lipolytic enzyme variants with increased lipolytic enzyme activity.Type: ApplicationFiled: February 7, 2013Publication date: June 20, 2013Applicant: NOVOZYMES A/SInventor: NOVOZYMES A/S
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Publication number: 20130157324Abstract: Disclosed herein are methods of producing alcohol esters during a fermentation by providing alcohol-producing microorganisms which further comprise an engineered polynucleotide encoding a polypeptide having lipase activity.Type: ApplicationFiled: June 15, 2012Publication date: June 20, 2013Applicant: BUTAMAX (TM) ADVANCED BIOFUELS LLCInventors: Robert DICOSIMO, Arthur Leo Kruckeberg, Thomas Edwin Van Aken
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Publication number: 20130149754Abstract: The present invention relates to a novel mutant strain of yeast, particularly a strain of Yarrowia lipolytica, which is capable of accumulating a large quantity of lipids. Said strain is deficient in the beta-oxidation of fatty acids and overexpresses the GPD1 gene. Preferably, the mutant strains, which do not express the GUT2 gene and which are deficient for the beta-oxidation of fatty acids, do not express the POX2 to POX5 genes, very preferably the POX2 to 6 genes. The invention also relates to a method for obtaining the strains of yeast according to the invention and to the use of said strains in the production of lipids.Type: ApplicationFiled: July 1, 2011Publication date: June 13, 2013Inventors: Thierry Dulermo, Jean-Marc Nicaud
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Publication number: 20130149753Abstract: A method of reducing process interruptions in biofuel production systems by reducing the amount of unwanted bacteria in the biofuel production system in which the reduction is effected by the use of an effective amount of one or more types of bacteriophages virulent for at least some strains of the unwanted bacteria.Type: ApplicationFiled: May 7, 2012Publication date: June 13, 2013Applicant: PHAGE BIOCONTROL RESEARCH, LLCInventors: Elizabeth J. Summer, Mei Liu
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Publication number: 20130152229Abstract: A ?5 fatty acid desaturase gene, a ?6 fatty acid desaturase gene, and a ?6 fatty-acid-chain elongase gene are isolated from a single species of Marchantiales. By introducing these genes into higher plants, transformed plants which can produce arachidonic acid and eicosapentaenoic acid (EPA) are obtained.Type: ApplicationFiled: January 31, 2011Publication date: June 13, 2013Applicant: Suntory Holdings LimitedInventor: Kanji Ohyama
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Publication number: 20130150604Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.Type: ApplicationFiled: February 7, 2013Publication date: June 13, 2013Applicant: DSM IP Assets B.V.Inventor: DSM IP Assets B.V.
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Publication number: 20130152230Abstract: Described nucleic acid molecules (and corresponding peptides) encode lyso-phosphatidylcholine (LPC) acyltransferases. Over-expression of the LPC acyltransferases in a cell may lead to enhanced production of PUFA, or other unusual fatty acids, and/or to increased oil content in the cell.Type: ApplicationFiled: January 18, 2013Publication date: June 13, 2013Applicants: Dow AgroSciences LLC, National Research Council of CanadaInventors: Qilin Chen, Jitao Zou, Zhifu Zheng, Jingyu Xu
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Patent number: 8460905Abstract: A method for degumming an oil composition comprises the steps of (a) providing an oil composition containing a quantity of phospholipids, (b) contacting said oil composition simultaneously with one or more phospholipase A enzymes and one or more phospholipase C enzymes, under conditions sufficient for the enzymes to react with the phospholipids to create phospholipid reaction products, and (c) separating the phospholipids reaction products from the oil composition, the remaining oil composition after the separation being a degummed oil composition, whereby during step (b) the reaction of said one or more phospholipase A enzymes proceeds at a faster rate than it would in the absence of said one or more phospholipase C enzymes, and wherein the reaction of step (b) continues for a duration of less than about one hour.Type: GrantFiled: September 11, 2007Date of Patent: June 11, 2013Assignee: Bunge Oils, Inc.Inventors: Christopher L. G. Dayton, Erin Marie Rosswurm, Flavio da Silva Galhardo
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Publication number: 20130143260Abstract: Provided are an alga in which productivity of a photosynthate is increased, and use of the alga. The alga of the present invention has an increased glutathione concentration in its chloroplast. A method of the present invention for producing biomass is a method for producing biomass with the use of the alga of the present invention or an alga produced by a method of the present invention for producing an alga.Type: ApplicationFiled: August 29, 2011Publication date: June 6, 2013Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCYInventors: Kenichi Ogawa, Masanobu Nishikawa
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Publication number: 20130143281Abstract: Disclosed are chimeric polyunsaturated fatty acid (PUFA) olyketide synthase (PKS) proteins and chimeric PUFA PKS systems, including chimeric PUFA PKS proteins and systems derived from Schizochytrium and Thraustochytrium. Disclosed are nucleic acids and proteins encoding such chimeric PUFA PKS proteins and systems, genetically modified organisms comprising such chimeric PUFA PKS proteins and systems, and methods of making and using such chimeric PUFA PKS proteins and systems.Type: ApplicationFiled: October 2, 2012Publication date: June 6, 2013Inventor: DSM IP Assets B.V.,
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Publication number: 20130143283Abstract: Methods and systems for processing organic waste material are provided. These methods and systems include integrating an anaerobic bio-digester and nutrient recovery module with a bio-production facility, which can locally provide feedstock for the bio-production facility, and can locally provide organic material for the anaerobic bio-digester. Methods and systems for integrating an anaerobic bio-digester with a gas cleaner are also provided, which can recover nutrients while cleaning the biogas produced by the anaerobic bio-digester.Type: ApplicationFiled: October 26, 2012Publication date: June 6, 2013Applicant: HIMARK BIOGAS INC.Inventor: HIMARK BIOGAS INC.
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Publication number: 20130143284Abstract: This disclosure describes genetically modified photosynthetic microorganisms, including Cyanobacteria, that contain one or more exogenous genes encoding a diacyglycerol acyltransferase, a phosphatidate phosphatase, and/or an acetyl-CoA carboxylase, and which are capable of producing increased amounts of fatty acids and/or synthesizing triglycerides.Type: ApplicationFiled: February 6, 2013Publication date: June 6, 2013Applicant: MATRIX GENETICS, LLCInventor: MATRIX GENETICS, LLC
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Publication number: 20130143282Abstract: Some aspects of this invention provide engineered microbes for oil production. Methods for microbe engineering and for use of engineered microbes are also provided herein. In some embodiments, microbes are provided that are engineered to modulate a combination of rate-controlling steps of lipid synthesis, for example, a combination of a step generating metabolites, acetyl-CoA, ATP or NADPH for lipid synthesis (a push step), and a step sequestering a product or an intermediate of a lipid synthesis pathway that mediates feedback inhibition of lipid synthesis (a pull step). Such push-and-pull engineered microbes exhibit greatly enhanced conversion yields and TAG synthesis and storage properties.Type: ApplicationFiled: October 19, 2012Publication date: June 6, 2013Applicant: Massachusetts Institute of TechnologyInventor: Massachusetts Institute of Technology
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Patent number: 8455669Abstract: The present invention relates to a new process for extracting fatty acids from aqueous biomass in a membrane contactor module. The present invention also relates to an integrated process combining biomass concentration and/or diafiltration and fatty acid extraction in said membrane contactor module.Type: GrantFiled: June 10, 2010Date of Patent: June 4, 2013Assignee: Due Miljo ASInventors: Andreia Manuela Martins Miranda, Eddy G. Torp, Inga Marie Aasen, Andrew Guy Livingston
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Publication number: 20130137149Abstract: The invention is directed to a genetically modified microorganism for the extracellular production of free fatty acids and esters thereof, wherein said microorganism is characterised by a modified lipid biosynthesis metabolic pathway: for example reduced fatty acyl-coA synthetase activity that enables the microorganism to over-produced and secrete of esters of fatty acids (Biodiesel) into the surrounding medium, using one or more of: glucose, starch, lignocellulose and a glycerol-based substrate, as a carbon source. The invention further provides a method for the extracellular production of free fatty acids and esters thereof, comprising the use of said genetically modified organism, and a growth medium adapted for said method.Type: ApplicationFiled: June 20, 2011Publication date: May 30, 2013Inventors: Ambareesh Govind Phadnavis, Peter Ruhdal Jensen
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Publication number: 20130137887Abstract: The embodiments disclosed herein relate to bacterial cell production of triacylglycerides from renewable biomass resources.Type: ApplicationFiled: October 19, 2012Publication date: May 30, 2013Applicant: Massachusetts Institute of TechnologyInventor: Massachusetts Institute of Technology
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Publication number: 20130130336Abstract: A method comprising providing a starting composition comprising a polyunsaturated fatty acid, a polyunsaturated fatty ester, a carboxylate salt of a polyunsaturated fatty acid, a polyunsaturated triglyceride, or a mixture thereof; self-metathesizing the starting composition or cross-metathesizing the starting composition with at least one short-chain olefin in the presence of a metathesis catalyst to form self-/cross-metathesis products comprising: cyclohexadiene; at least one olefin; and one or more acid-, ester-, or salt-functionalized alkene; and reacting cyclohexadiene to produce at least one cycloalkane or cycloalkane derivatives.Type: ApplicationFiled: January 15, 2013Publication date: May 23, 2013Applicant: Energy & Environmental Research Center FoundationInventor: Energy & Environmental Research Center Foundation
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Publication number: 20130130335Abstract: Methods of producing renewable materials may include consuming a fermentation feedstock with a fermentation organism to produce a renewable material in fermentation broth; water may then be separated from the feedstock or broth using one or more phase separations, or the renewable material may be concentrated from the feedstock or broth using one or more phase separations. Methods of producing biofuel components may include consuming a lignocellulosic or sugar fermentation feedstock with a fermentation organism to produce either ethanol or butanol in fermentation broth; cooling the feedstock or broth to solidify at least some water therein; and separating the solidified water from the feedstock or broth using a solid-liquid phase separation.Type: ApplicationFiled: November 21, 2012Publication date: May 23, 2013Applicant: BP Corporation North America Inc.Inventors: Glen Austin, Binita X. Bhattacharjee, Leslie W. Bolton, Jacob Borden, Martin E. Carrera, Amit A. Gokhale, Chris Horler, Aidan Hurley, Eric T. Mack
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Publication number: 20130129775Abstract: Disclosed here are compositions rich in omega-7 fatty acids, including palmitoleic acid, and products rich in omega-7 fatty acids derived from algal biomass. The algae and/or compositions rich in omega-7 fatty acids may be used in products or as ingredients of products. Methods and systems for increasing the production or concentration of omega-7 fatty acids, and isolating omega-7 fatty acids from algal biomass are also disclosed herein.Type: ApplicationFiled: November 19, 2012Publication date: May 23, 2013Applicant: HELIAE DEVELOPMENT, LLCInventor: Heliae Development, LLC
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Publication number: 20130131361Abstract: A medical application of N-oleoyl-dopamine and 3?-O-methyl-N-oleoyl-dopamine for the production of a medication for treatment or prophylaxis of disturbances or states due to deficiency of dopamine, preferably chosen from the group covering oxygen deficiencies (hypoxia) in anesthetized and awake mammals, breathing with atmospheric oxygen (normoxia), for the respiratory responses to hypoxia in mammals, lack of dopamine binding to the membrane system in the mammalian brain, disturbances in metabolic pathways of dopamine metabolism, states of dopamine deficiencies provoked by morbid or genetic factors as well as under condition connected to the physiological process of aging of the organism, through the systemic introduction of the compound to the mammalian organism. A method of production of 3?-O-methyl-N-oleoyl-dopamine in vitro is also disclosed.Type: ApplicationFiled: November 23, 2011Publication date: May 23, 2013Applicants: Uniwersytet Warszawski, Wydzial Chemii, Instytut Medycyny Doswiadczalnej i Klinicznej im. M. Mossakowskiego PANInventors: Mieczyslaw Pokorski, Dominika Zajac, Zbigniew Czarnocki, Piotr Roszkowski
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Publication number: 20130130334Abstract: A fuel cell comprising an anode electrode, a cathode electrode and a reference electrode electronically connected to each other; a first biocatalyst comprising a consolidated bioprocessing organism (e.g., a cellulomonad or clostridium or related strains, such as Cellulomonas uda (C. uda), C. lentocellum, A. cellolulyticus, C. cellobioparum, alcohol-tolerant C. cellobioparum, alcohol-tolerant C. uda, Clostridium cellobioparum (C. cellobioparum) and combinations thereof) capable of fermenting biomass (e.g., cellulosic biomass or glycerin-containing biomass) to produce a fermentation byproduct; and a second biocatalyst comprising an electricigen (e.g., Geobacter sulfurreducens) capable of transferring substantially all the electrons in the fermentation byproduct (e.g., hydrogen, one or more organic acids, or a combination thereof) to the anode electrode to produce electricity is disclosed. Systems and methods related thereto are also disclosing a consolidated bioprocessing organism.Type: ApplicationFiled: March 17, 2011Publication date: May 23, 2013Applicant: Board of Trustees of Michigan State UniversityInventors: Gemma Reguera, Allison Speers, Jenna Young
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Publication number: 20130131173Abstract: The invention relates to a microbial biomass composition produced from a heterotrophic fermentation whose fatty acid profile exhibits: an eicosapentaenoic acid (EPA) to arachidonic acid (ARA) ratio of about 11:1 or more; an EPA to total co-concentrating fatty acid ratio of about 8:1 or more, extract compositions, and methods of producing such compositions.Type: ApplicationFiled: June 9, 2011Publication date: May 23, 2013Applicant: Photonz Corporation LimitedInventors: Hywel David Griffiths, Karl Thomas Geiringer, Mark Humphrey Dines
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Publication number: 20130122558Abstract: The present invention relates to mutant ?5 desaturases, which have the ability to convert dihomo-?-linolenic acid [DGLA; 20:3 ?-6] to arachidonic acid [ARA; 20:4 ?-6] and/or eicosatetraenoic acid [ETA; 20:4 ?-3] to eicosapentaenoic acid [EPA; 20:5 ?-3] and which possess at least one mutation within the HPGG motif of the cytochrome b5-like domain. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding ?5 desaturases, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”] using these mutant ?5 desaturases in oleaginous yeast, are disclosed.Type: ApplicationFiled: January 8, 2013Publication date: May 16, 2013Applicant: E I DU PONT DE NEMOURS AND COMPANYInventor: E I Du Pont De Nemours And Company
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Publication number: 20130123525Abstract: A method for lowering saturated fatty acid content, the method comprising concentrating polyunsaturated fatty acid using a lipase having low reactivity for the polyunsaturated fatty acid to react with a glyceride containing a polyunsaturated fatty acid; wherein the lipase reaction is performed at a temperature of not more than 25° C.Type: ApplicationFiled: May 27, 2011Publication date: May 16, 2013Applicant: NIPPON SUISAN KAISHA, LTD.Inventors: Hideo Ikemoto, Nobushige Doisaki, Yasuo Umehara
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Publication number: 20130122557Abstract: The invention is directed to isolated microorganisms, as well as biomasses, cultures, microbial oils, and compositions thereof. The invention also provides methods of producing the microbial oils and methods of using the isolated microorganisms, biomasses, and microbial oils.Type: ApplicationFiled: March 11, 2011Publication date: May 16, 2013Inventors: Kirk E. Apt, William R. Barclay, Paul Warren Behrens
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Patent number: 8440435Abstract: The present application provides a method of reducing and/or removing diglyceride from an edible oil, comprising admixing an edible oil with an acyl acceptor substrate and a diglyceride:glycerol acyltransferase, wherein the diglyceride:glycerol acyltransferase is characterized as an enzyme which in an edible oil is capable of transferring an acyl group from a diglyceride to glycerol. The diglyceride:glycerol acyltransferase can comprise the amino acid sequence motif GDSX. The present invention also relates to the use of a diglyceride:glycerol acyltransferase in the manufacture of an edible oil, for reducing and/or removing diglyceride from said edible oil and to the use of said enzyme in the manufacture of a foodstuff comprising an edible oil for improving the crystallization properties of said foodstuff.Type: GrantFiled: June 25, 2009Date of Patent: May 14, 2013Assignee: DuPont Nutrition Biosciences ApSInventors: Paul Wassell, Jørn Borch Søe, Jørn Dalgaard Mikkelsen, Anna Cecilie Jentoft Kristensen