Abstract: Described herein are engineered microalgae that exhibit enhanced lipid production during exponential growth. Such engineered microalgae are useful, for example, for the production of biofuels.

Abstract: Disclosed are an amphipathic peptide-lipase conjugate with enhanced lipase activity, a polynucleotide coding for the conjugate, an expression vector carrying the polynucleotide, a transformant anchoring the expression vector therein, a method for preparing the conjugate, a lipolysis method using the conjugate, and a method for producing biodiesel using the lipase.

Abstract: This invention concerns a method for the targeted integration of at least three copies of a gene of interest in the genome of a Yarrowia strain including the steps of: (a) cultivating a Yarrowia strain, said strain including a deletion among at least three genes, the phenotype associated with each of these deletions corresponding to an auxotrophy or to a dominant character for this strain; (b) transforming said Yarrowia strain thus obtained with at least three recombinant vectors that include selection markers allowing, for this strain, the complementation of auxotrophy and, potentially, of the dominant character resulting from each of these deletions; and (c) selecting, on a minimum medium, the yeasts having integrated said at least three recombinant vectors.

Abstract: Described herein are methods and systems that can provide independent energy generation as well as systems for the generation of other useful products, such as chemicals. In addition, the systems and methods can provide ways of harnessing the potential of biomass feedstock to generate of multitude of products including green crude, biogas, electricity, heat, fatty acids, biodiesel, ammonia, and chemical products. In many cases, the systems and methods herein utilize anaerobic microorganisms, including aquatic and ruminant organisms, to digest material and create products.

Abstract: The invention provides methods and systems for the production of lipid products from a gaseous substrate using a two stage fermentation process. The method comprises providing a gaseous substrate comprising CO or CO2 and H2 or mixtures thereof, to a first bioreactor containing a culture or one or more microorganisms, and fermenting the substrate to produce acetate. The acetate from the first bioreactor is then provided to a second bioreactor, where it is used as a substrate for fermentation to lipids by one or more microalgae.

Abstract: The invention provides method and compositions to minimize the chlorophyll antenna size of photosynthesis by decreasing TLA1 gene expression, thereby improving solar conversion efficiencies and photosynthetic productivity in plants, e.g., green microalgae, under bright sunlight conditions.

Abstract: The present disclosure provides compositions and methods for the fermentation of cellodextrins and ?-D-glucose. Host cells and recombinant polypeptides having glucose mutarotase activity are provided. Additionally, methods for improving cell growth, production of chemicals, and consumption of cellodextrins and ?-D-glucose during fermentation of mixtures containing cellodextrins and ?-D-glucose are provided.

Abstract: A process for the fermentative production of at least one organic compound having at least 3 C atoms or having at least 2 C atoms and at least one 1 N atom, comprising the following steps: a1) milling a starch feedstock, thus obtaining a millbase which comprises at least part of the nonstarchy solid constituents of the starch feedstock; a2) suspending the millbase in an aqueous liquid and hydrolysis of the starch portion in the millbase by enzymatic liquefaction and, if appropriate, subsequent saccharification, whereby a first liquid (1) which comprises mono- or oligosaccharides is obtained; and b) addition of the liquid (1) which comprises mono- or oligosaccharides together with metabolizable mono-, di- or oligosaccharides or together with a composition which comprises metabolizable mono-, di- or oligosaccharide in a concentration of at least 50% by weight and which is essentially free from solids which are insoluble in water to a fermentation medium comprising a microorganism which is capable of overproduc

Abstract: The present compositions and methods relate to a lipase cloned from Geobacillus stearothermophilus, polynucleotides encoding the lipase, and methods of use thereof. The compositions and methods have particular application in detergent cleaning compositions and methods.

Abstract: An energy efficient process for the preparation of marine microalgae Chlorella fatty acid methyl ester (CME) from hydrolysate of deoiled cake of Jatropha (JOCH) and crude glycerol co-product stream (GL7 and GL8) along with seawater diluted with tap water (1:2). A small part of the crude glycerol layer in case of JME is processed to recover glycerol for glycerol washing and the otherwise problematic still bottom is utilized for microbial synthesis of PHAs and the rest is utilized for Microalgal conversion of JME byproducts into CME. The remaining part of the methanol-depleted glycerol layer is utilized, along with hydrolysate of the Jatropha deoiled cake (JOCH), for single-stage Microalgal production of lipids by a marine Microalgal isolate (Chlorella sp.) without the need for any other nutrients. Waste streams from the microalgal processes can be discharged directly into agricultural fields as biofertilizer or recycled back in the mass cultivation.

Abstract: Methods of and devices for recovering oil and protein and increasing production yields using two centrifuges in series or a centrifuge with dual oil and protein separating functions in a dry mill are provided.

Abstract: The invention is directed to isolated microorganisms, as well as biomasses, cultures, microbial oils, and compositions thereof. The invention also provides methods of producing the microbial oils and methods of using the isolated microorganisms, biomasses, and microbial oils.

Abstract: Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 50 weight percent of eicosapentaenoic acid in the total oil fraction are described. These strains over-express heterologous ?9 elongases, ?8 desaturases, ?5 desaturases, ?17 desaturases, ?12 desaturases and C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases. Gene knockouts are also described, as are methods for producing EPA within host cells, and products comprising EPA from the optimized Yarrowia lipolytica strains.

Abstract: Disclosed herein are transformed Yarrowia lipolytica comprising an exogenous polynucleotide encoding a polypeptide having sucrose invertase activity. Also disclosed are methods of using the transformed Y. lipolytica.

Abstract: Disclosed are novel Pseudomonas aeruginosa strains capable of producing in high yield and preparation methods thereof. The strains anchor an expression vector carrying either or both of a nucleotide sequence coding for acetyl-CoA carboxylase carboxytransferase subunit alpha of Pseudomonas aeruginosa and a nucleotide sequence coding for malonyl-CoA-[acyl-carrier-protein] transacylase of Pseudomonas aeruginosa, and/or a nucleotide sequence coding for acyl-acyl carrier protein thioesterase of Streptococcus pyogenes. The recombinant Pseudomonas aeruginosa strains are genetically stable and have high lipid or fatty acid contents, thus being applicable to the mass production of fatty acids.

Abstract: The present invention relates to processes for producing industrial products such as hydrocarbon products from non-polar lipids in a vegetative plant part. Preferred industrial products include alkyl esters which may be blended with petroleum based fuels.

Abstract: High cell density fermentations of wild-type organisms can result in increased viscosity due to the production of exocellular polysaccharides. Mutant microorganisms with a dry morphology, resulting from reduced exocellular polysaccharide formation, were isolated and characterized. The exocellular polysaccharide composition for these modified microorganisms is shown to be different than the polysaccharide composition of the wild type microorganism. In addition to reduced exocellular polysaccharide formation, dry morphology mutants of multiple strains show reduced viscosity, improved oxygen mass transfer, and improved fatty acid fermentation yield on carbon.

Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.

Abstract: The invention provides methods and systems for the production of lipid products from a gaseous substrate using a two stage fermentation process. The method comprises providing a gaseous substrate comprising CO, CO2 and H2 or mixtures thereof, to a first bioreactor containing a culture or one or more microorganisms, and fermenting the substrate to produce a product comprising acetate. The acetate from the first bioreactor is then provided to a second bioreactor, where it is used as a substrate for fermentation to lipids by one or more yeasts.

Abstract: Vegetable oils having a low level of glycidol esters are disclosed.

Abstract: The invention relates to a method for producing at least one organic compound comprising at least 3 C-atoms or at least 2 C-atoms and at least 1 N-atom by fermentation. Said method comprises the following steps: i) a starch source is ground in order to obtain a grinding material which contains at least one part of the non-starch containing solid components of the starch source; ii) the grinding material is suspended in an aqueous liquid in an amount such that the dry mass content in the suspension is at least 45 wt.

Abstract: This invention relates to a lignin sorbent, a lignin removal unit, a biorefinery, a process for removing lignin, a process for binding lignin, and a renewable material. The lignin sorbent includes a substrate, and a lignin binding material dispersed with respect to the substrate. The lignin binding process includes the step of adding a lignin binding material to an input stream, and the step of converting the input stream into a renewable material.

Abstract: The invention provides a recombinant cell having a nucleotide sequence encoding a polypeptide which is a lipase having at least 40% amino acid sequence identity to a polypeptide having SEQ ID NO:1, and methods of using the recombinant cell to produce triacylglycerols or to increase oil production by the cell.

Abstract: Bioreactors, and particularly, photobioreactors having a reactor chamber and surge driver, and methods for using these devices, for example, for the production of carbon-based products are provided. The reactor chamber provides a housing for microorganisms and culture medium. The surge driver produces a surge of the microorganisms and/or culture medium in the reactor chamber.

Abstract: Disclosed are methods for obtaining oil from maize, involving grinding maize kernels to form flour, adding water to the flour to form a slurry, and incubating the slurry with ?-amylase for about 10 minutes to about 180 minutes at a temperature of about 75° to about 120° C. and at a pH of about 3 to about 7 to form a mash, cooling the mash to about 15° C. to about 40° C. and adding a nitrogen source, glucoamylase, yeast, acid protease, and cell-wall polysaccharide-degrading enzymes to form a beer containing ethanol and oil, wherein the beer has a pH of about 3 to about 7, and recovering oil from the beer.

Abstract: Provided herein are exemplary genes, constructs and methods for the formation of triacylglycerols (TAGs). The exemplary genes include a phosphatic acid phosphohydrolase (PA Hydrolase) gene, a diacylglycerol o-acyltransferase (DAGAT2A) gene, and a phospholipid:diacylglycerol acyltransferase (LROI) gene.

Abstract: This invention is a system for the production of biomass from photosynthesizing microorganisms that includes a photobioreactor comprising a transparent panel made from two transparent sheets with a separation between them, with top and bottom openings and with transparent, parallel subdivisions that form a panel of vertically arranged, transparent cells, where each transparent cell has a top opening and a bottom opening; a lower recirculation chamber in fluid contact with the bottom openings of the transparent panel; an upper recirculation chamber in fluid contact with the top openings of the transparent panel; a gas distribution tube externally arranged along the edge of said transparent panel; where said gas distribution tube comprises gas injectors arranged in fluid contact with the interior of a plurality of transparent cells; and a supporting structure that supports the transparent panel, the lower recirculation chamber, the upper recirculation chamber and the air distribution tube.

Abstract: A method for producing a fatty acid, which comprises adding an organic solvent to a culture of an alga obtained by culturing the alga in a culture medium, and stirring the culture medium to allow a transesterification or hydrolysis reaction of a lipid, and collecting a fatty acid ester or a fatty acid from the reaction mixture.

Abstract: The invention provides non-naturally occurring microbial organisms containing a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organisms selectively produce a fatty alcohol, fatty aldehyde or fatty acid of a specified length. Also provided are non-naturally occurring microbial organisms having a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organisms further include an acetyl-CoA pathway. In some aspects, the microbial organisms of the invention have select gene disruptions or enzyme attenuations that increase production of fatty alcohols, fatty aldehydes or fatty acids. The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde or a fatty acid.

Abstract: A method for increasing cuticular wax biosynthesis of plant includes transforming a plant cell with the recombinant vector containing MYB96 (myb domain protein 96) gene from Arabidopsis thaliana. A method for producing a transgenic plant with increased cuticular wax biosynthesis includes transforming a plant cell with the recombinant vector containing the MYB96 gene, and regenerating the transformed plant cell into the transgenic plant. A plant and a seed with increased cuticular wax biosynthesis are produced by the method. A method for producing a biofuel using a cuticular wax includes separating and purifying a cuticular wax from the plant. A composition for increasing cuticular wax biosynthesis of plant includes the MYB96 gene.

Abstract: A novel strain of microalga of the Odontella genus, capable of growing in mixotrophic mode, and a method for selecting and culturing such a strain allow production of polyunsaturated fatty acids, notably EPA and DHA, in heterotrophic or mixotrophic mode.

Abstract: The invention relates to the utilisation of fatty acid feedstocks with substantial free fatty acid content in the production of biodiesel by the use of microbial enzymes.

Abstract: The present disclosure is directed to novel polynucleotide sequences for use in Nannochloropsis gaditana. The novel polynucleotide sequences include control sequences and coding sequences. Also disclosed are novel gene expression constructs wherein N. gaditana promoters/control regions are operatively linked to N. gaditana or non-N. gaditana coding sequences. These novel polynucleotide sequences and expression constructs can be introduced into N. gaditana and can recombine into the N. gaditana genome. Expression from these polynucleotide sequences and expression constructs can enhance N. gaditana biomass and/or lipid biosynthesis. Also disclosed are methods for modifying N. gaditana, for example by stably transforming N. gaditana with nucleic acid sequences, growing the modified N. gaditana, and obtaining biomass and biofuels from the modified N. gaditana.

Abstract: Exemplary methods for increasing TAG production in an algal cell during imbalanced growth conditions are provided. Some methods comprise knocking out an AOX gene, wherein the AOX gene produces an amino acid sequence having substantial similarity to the amino acid sequence of SEQ. ID. NO. 2. In further methods, the algal cell may be of genus Nannochloropsis. The AOX gene may be replaced by a construct having a nucleotide sequence having substantial similarity to SEQ ID. NOS. 3 through 5 (inclusive), wherein each of the sequences are next to or in close proximity to one another in a linear fashion. In some methods, the AOX gene may be replaced via homologous recombination. As a result, lipid production by the selected recombinant algal cell may be increased over that produced by a wild-type algal cell.

Abstract: The invention relates to a novel strain of Schizochytrium sp. that can produce large quantities of squalene, the methods for the production of lipid compounds of interest using said strain and the products and compositions prepared with said strain.

Abstract: Disclosed is a plant for producing microalgae biofuel. The plant includes a plant space part having an internal space, a culture part provided in the internal space of the plant space part to culture microalgae by continuously circulating a fluid, which includes the microalgae and is supplied from an outside, at positions different from each other, and a temperature adjusting part to adjust a temperature value of the internal space in the plant space part to a temperature value in a preset temperature value range.

Abstract: The present invention relates generally to microalgae strains that are tolerant to high salinity, the products derived from the high salinity tolerant microalgae strains, and methods of producing high salinity tolerant microalgae strains and their products.

Abstract: A process for the modification of a substrate comprising passing the substrate through a packed bed column of a specific volume of immobilized enzyme wherein the substrate enters the column at or near one end of the column (the ‘inlet end’) and the modified substrate exits at or near the opposite end of the column (the ‘outlet end’), a portion of the volume of immobilized enzyme is periodically removed at or near to the inlet end of the column, and an equivalent portion of immobilized enzyme is periodically added at or near to the outlet end of the column.

Abstract: Engineered strains of the oleaginous yeast Yarrowia lipolytica are disclosed herein that are capable of producing microbial oil comprising greater than 25 weight percent of eicosapentaenoic acid [“EPA”], an omega-3 polyunsaturated fatty acid, measured as a weight percent of dry cell weight.

Abstract: The present invention relates to a host cell deficient in an essential gene, comprising a vector, said vector comprising at least said essential gene and an autonomous replication sequence, wherein the host cell is a filamentous fungal cell. The invention also relates to a host cell deficient in an essential gene, comprising a vector, said vector comprising at least said essential gene and an autonomous replication sequence, wherein the host cell comprises a recombinant polynucleotide construct comprising a polynucleotide encoding a biological compound of interest or a compound involved in the synthesis of a biological compound of interest.

Abstract: Provided are methods and compositions for higher yields through the addition of pretreatment/hydrolysis extracts in the fermentation process. These fractions increase the rate of growth for biocatalysts and result in improved composition of fermentation end products.

Abstract: The present invention relates to processes and systems for the production of fermentation products such as alcohols. The present invention also provides methods for separating feed stream components for improved biomass processing and productivity.

Abstract: The present invention provides novel diacylglycerol acyltransferase (DGAT) genes, including novel genes encoding localization peptides. The present invention also provides recombinant cells, such as algae, transformed with DGAT genes and methods of using such recombinant cells to produce triglyceride.

Abstract: Disclosed herein are obligate heterotrophic microalgae cells containing an exogenous gene. In some embodiments the gene is a sucrose utilization gene, and further disclosed are methods of manufacturing triglyceride oils using sugar cane or sugar beets as a feedstock in a heterotrophic fermentation. In other embodiments the feedstock is depolymerized cellulosic material. Also disclosed are cells that produce medium chain fatty acids at levels not produced in non-recombinant cells of the same species and genus.

Abstract: Diols produced in fermentation are processed in broth by esterification of the product diol with a carboxylic acid (e.g., fatty acid) and a catalyst (e.g., lipase) capable of esterifying the product diol, such as 1,3-propanediol, with the carboxylic acid to form the diol esters. The diol esters can be extracted from the broth, and the product diol recovered from the diol esters. The carboxylic acid can also serve as an extractant for removal of the diol esters from the fermentation medium.

Abstract: Disclosed are processes for producing lipids for biofuel or lubricant and Streptomyces bacteria used in the processes. The processes include steps whereby bacterial cells of the germs Streptomyces are cultivated in a medium comprising organic waste(s) or residue(s) or mixtures thereof as carbon and/or nutrient source(s), wherein the organic waste(s) or residue(s) comprise lignocellulosic waste(s) or lignocellulosic residue(s), recovering lipids from the cells of the bacteria or from the cultivation medium, and using the recovered lipids or a fraction thereof as biofuel and/or lubricant, or as a starting material for biofuel and/or lubricant production.

Abstract: The invention provides methods of manufacturing biodiesel and other oil-based compounds using glycerol and combinations of glycerol and other feedstocks as an energy source in fermentation of oil-bearing microorganisms. Methods disclosed herein include processes for manufacturing high nutrition edible oils from non-food feedstock materials such as waste products from industrial waste transesterification processes. Also included are methods of increasing oil yields by temporally separating glycerol and other feedstocks during cultivation processes. Also provided herein are oil-bearing microbes containing exogenous oil production genes and methods of cultivating such microbes on glycerol and other feedstocks.

Abstract: The invention teaches the obtained specifications and process of production of engine worthy marine microalgal fatty acid methyl ester (biodiesel) using naturally occurring marine microalgal mats and also marine microalgae cultivated in cost-effective manner in solar salt pans. Utility of co-product streams adds to the attractiveness of the invention.

Abstract: The present invention provides novel fatty acid desaturases genes used for synthesis of polyunsaturated fatty acids, especially omega-3 desaturases (FADS15). The present invention also provides nucleic acid sequence coding the above-described desaturases, expression vector of the above-described desaturases and recombinant microorganism expressing above-described desaturases.

Abstract: The present invention provides a bioreactor having a reactor chamber and one or more support chambers. The reactor chamber can have one or more flexible walls for enclosing microorganisms and culture medium. The reactor chamber provides an enclosure for microorganism and culture medium. The support chamber can also have one or more flexible walls. When inflated to a predetermined amount, the support chamber causes the microorganisms and culture medium to distribute to a substantially even depth across the reactor chamber.