Ketone Patents (Class 435/148)
  • Patent number: 11000558
    Abstract: The present invention is directed to the direct application of beneficial or probiotic bacteria to the vaginal niche for maintenance of a healthy vaginal microbiota and to help restore an unbalanced vaginal microbiota. The application is based on the use of selected Lactobacillus strains as anti-pathogenic agents, in particular L. pentosus and/or L. plantarum, against common vaginal pathogens, whereby produced acids such as lactic acid are important antimicrobial factors.
    Type: Grant
    Filed: March 21, 2017
    Date of Patent: May 11, 2021
    Inventors: Sarah Lebeer, Ingmar Claes, Eline Oerlemans, Marianne Van Den Broek
  • Patent number: 10995321
    Abstract: The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
    Type: Grant
    Filed: October 17, 2019
    Date of Patent: May 4, 2021
    Assignee: Codexis, Inc.
    Inventors: Christopher K. Savile, Vesna Mitchell, Xiyun Zhang, Gjalt W. Huisman
  • Patent number: 10941429
    Abstract: Disclosed are methods, compositions, proteins, nucleic acids, cells, vectors, compounds, reagents, and systems for the preparation of kavalactones, flavokavains, and kavalactone and flavokavain biosynthetic intermediates using enzymes expressed in heterologous host cells, such as microorganisms or plants, or using in vitro enzymatic reactions. This invention also provides for the expression of the enzymes by recombinant cell lines and vectors. Furthermore, the enzymes can be components of constructs such as fusion proteins. The kavalactones produced can be utilized to treat anxiety disorder, insomnia, and other psychological and neurological disorders. The flavokavains produced can be utilized to treat various cancers including colon, bladder, and breast cancers.
    Type: Grant
    Filed: January 16, 2019
    Date of Patent: March 9, 2021
    Assignee: Whitehead Institute for Biomedical Research
    Inventors: Tomás Pluskal, Jing-Ke Weng
  • Patent number: 10907136
    Abstract: It is intended to provide an efficient method for expressing a protein of interest as an active soluble recombinant protein in a transformant. The present invention provides a method for producing an enzyme having improved activity per recombinant as compared with a reference form, comprising the steps of: (1) preparing recombinants each expressing a mutant having an amino acid sequence derived from the amino acid sequence of the reference form by the substitution of one or more cysteine residues by other amino acid residues; (2) selecting a plurality of mutants that exhibit 50% or more activity per recombinant relative to 100% activity of the reference form; and (3) expressing a mutant in which corresponding amino acid residues are substituted at two or more positions among the respective positions of the substituted amino acid residues of the mutants selected in step (2).
    Type: Grant
    Filed: February 28, 2019
    Date of Patent: February 2, 2021
    Assignee: Mitsubishi Chemical Corporation
    Inventors: Fujio Yu, Wataru Mizunashi
  • Patent number: 10889840
    Abstract: The present invention relates to new methylglyoxal reductase (MGR) enzymes which are useful for efficiently converting methylglyoxal into hydroxyacetone. The invention more particularly relates to a method for efficiently converting methylglyoxal into hydroxyacetone using said enzymes, to a method for producing 1,2-propanediol using a microorganism overexpressing said enzymes, and to said microorganism.
    Type: Grant
    Filed: December 29, 2017
    Date of Patent: January 12, 2021
    Assignee: METABOLIC EXPLORER
    Inventors: Laetitia Faure, Gwenaëlle Corre, Céline Raynaud, Laurence Dumon-Seignovert
  • Patent number: 10877035
    Abstract: X-ray fluorescence (XRF) spectrometry has been used for detecting binding events and measuring binding selectivities between chemicals and receptors. XRF may also be used for estimating the therapeutic index of a chemical. For estimating the binding selectivities of a chemical versus chemical analogs, for measuring post translational modification of proteins, and for drug manufacturing.
    Type: Grant
    Filed: April 22, 2015
    Date of Patent: December 29, 2020
    Assignee: ICAGEN, LLC
    Inventors: Eva R. Birnbaum, Andrew T. Koppisch, Sharon M. Baldwin, Benjamin P. Warner, T. Mark McCleskey, Jennifer A. Berger, Jeffrey J. Stewart, Michael N. Harris, Anthony K. Burrell
  • Patent number: 10801020
    Abstract: Provided are a hexuronate C4-epimerase variant with improved activity in converting D-fructose by D-tagatose of hexuronate C4-epimerase and a method for production of D-tagatose using them.
    Type: Grant
    Filed: July 31, 2017
    Date of Patent: October 13, 2020
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Sung Jae Yang, Young Mi Lee, Il Hyang Park, Chan-Hyoung Lee, Hyun Kug Cho, Seong Bo Kim, Yang Hee Kim, Seung Won Park
  • Patent number: 10772913
    Abstract: Methods and compositions related to the use of a protein with kynureninase activity are described. For example, in certain aspects there may be disclosed a modified kynureninase capable of degrading kynurenine. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer with kynurenine depletion using the disclosed proteins or nucleic acids.
    Type: Grant
    Filed: April 2, 2019
    Date of Patent: September 15, 2020
    Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
    Inventors: George Georgiou, Everett Stone
  • Patent number: 10731137
    Abstract: The present invention relates to new lactaldehyde reductase (LAR) enzymes useful for the production of 1,2-propanediol and to microorganisms overexpressing said enzymes. The invention also relates to a method for producing 1,2-propanediol by converting lactaldehyde into 1,2-propanediol with said enzymes.
    Type: Grant
    Filed: September 10, 2015
    Date of Patent: August 4, 2020
    Assignee: METABOLIC EXPLORER
    Inventors: Gwénaëlle Corre, Pascale Aliprandi
  • Patent number: 10724008
    Abstract: The invention relates to ketoreductases and the use thereof. The ketoreductases of the invention are particularly useful for enzymatically catalyzing the reduction of ketones to chiral secondary alcohols.
    Type: Grant
    Filed: August 30, 2018
    Date of Patent: July 28, 2020
    Assignee: C-LEcta GmbH
    Inventors: Ramona Schmiedel, Andreas Vogel, Sabrina Koepke, Rico Czaja, Claudia Feller, Hedda Merkens, Kamila Rzeznicka, Daniel Schwarze, Thomas Greiner-Stoeffele, Andreas Petri, Marc Struhalla
  • Patent number: 10590406
    Abstract: The invention provides a recombinant, carboxydotrophic Clostridium bacterium that expresses one or more of pyruvate:ferredoxin oxidoreductase (EC 1.2.7.1), acetolactate synthase (EC 2.2.1.6), and acetolactate decarboxylase (EC 4.1.1.5). The invention further provides a method of producing a fermentation product by fermenting the recombinant bacterium in the presence of a gaseous substrate comprising CO to produce one or more of ethanol, butanol, isopropanol, isobutanol, higher alcohols, butanediol, 2,3-butanediol, succinate, isoprenoids, fatty acids, biopolymers, and mixtures thereof.
    Type: Grant
    Filed: December 7, 2015
    Date of Patent: March 17, 2020
    Assignee: LANZATECH NEW ZEALAND LIMITED
    Inventors: Michael Koepke, Alexander Paul Mueller, Loan Phuong Tran
  • Patent number: 10538797
    Abstract: The invention relates to a method for producing flavone glycoside dihydrochalcones, having the following steps: (a) providing a transgenic microorganism containing (i) a first nucleic acid portion (A) containing a gene which codes for a bacterial chalcone isomerase and (ii) a second nucleic acid portion (B) containing a gene which codes for a bacterial enoate reductase, (b) adding one or more flavone glycosides to the transgenic microorganism under conditions which allow the simultaneous isomerization and reduction of the flavone glycoside into the flavone glycoside dihydrochalcone, and optionally (d) isolating and purifying the final product, wherein the nucleic acid portion (A) (1) is a nucleotide sequence according to SEQ ID NO:1, in which the nucleic acid portion (A?) according to SEQ ID NO:3 has been cut out, or (2) is an amino acid sequence according to SEQ ID NO:2, in which the amino acid portion (A?) according to SEQ ID NO:4 has been cut out.
    Type: Grant
    Filed: June 27, 2015
    Date of Patent: January 21, 2020
    Assignee: SYMRISE AG
    Inventors: Maren Thomsen, Jakob Ley, Egon Gross, Winfried Hinrichs, Uwe Bornscheuer
  • Patent number: 10494615
    Abstract: The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
    Type: Grant
    Filed: June 28, 2018
    Date of Patent: December 3, 2019
    Assignee: Codexis, Inc.
    Inventors: Christopher K. Savile, Vesna Mitchell, Xiyun Zhang, Gjalt W. Huisman
  • Patent number: 10035988
    Abstract: The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
    Type: Grant
    Filed: February 27, 2017
    Date of Patent: July 31, 2018
    Assignee: Codexis, Inc.
    Inventors: Christopher K. Savile, Vesna Mitchell, Xiyun Zhang, Gjalt W. Huisman
  • Patent number: 10031125
    Abstract: Methods for diagnosing, treating, and monitoring the treatment of invasive aspergillosis (IA) are described. The methods can include detecting the presence of one or more volatile organic compounds (VOCs) in the breath of subjects suspected of having IA.
    Type: Grant
    Filed: September 6, 2013
    Date of Patent: July 24, 2018
    Assignees: The Brigham and Women's Hospital, Inc., The Charles Stark Draper Laboratory, Inc.
    Inventors: Sophia Koo, Horatio R. Thomas, James Constantine Comolli, Preshious Rearden, Lindsey R. Baden, Francisco M. Marty
  • Patent number: 9951354
    Abstract: The present invention provides methods and compositions for producing ?-phellandrene hydrocarbons from cyanobacteria.
    Type: Grant
    Filed: February 6, 2013
    Date of Patent: April 24, 2018
    Assignee: The Regents of the University of California
    Inventors: Anastasios Melis, Fiona K. Davies, Hsu-Ching Chen Wintz, Andreas Zurbriggen
  • Patent number: 9914984
    Abstract: The present invention relates to a composition for epimerization of a non-phosphorylated hexose, comprising sugar epimerases derived from thermophiles, and a method for preparing a non-phosphorylated hexose epimer using the composition. The sugar epimerases derived from thermophiles according to the present invention can effectively catalyze an epimerization reaction of a non-phosphorylated hexose and can easily produce an epimer form of the non-phosphorylated hexose, in particular a rare sugar hexose, and thus can be usefully utilized in the pharmaceutical and food industry.
    Type: Grant
    Filed: November 19, 2014
    Date of Patent: March 13, 2018
    Assignee: CJ CheilJedang Corporation
    Inventors: Dong Woo Lee, Sun Mi Shin, Yong Jik Lee, Sang Jae Lee
  • Patent number: 9890384
    Abstract: Carboxydotrophic acetogenic microorganisms do not produce MEK and/or 2-butanol. They lack the biosynthesis pathways to make these products. In addition, they produce the intermediate (R,R)-2,3-butanediol whereas the production of MEK and 2-butanol requires production of the intermediate (R,S)-2,3-butanediol. Nonetheless, the production of MEK and/or 2-butanol can be accomplished using recombinant microorganisms adapted to express or overexpress key enzymes in the MEK and/or 2-butanol biosynthesis pathways. Such microorganisms, such as the carboxydotrophic acetogen Clostridium autoethanogenum, can ferment substrates comprising CO. The overall scheme involves the production of 2-butanol from (R,S)-2,3-butanediol and the conversion of (R)-acetoin to (S)-2,3-butanediol. These steps are involved in the production of both MEK and 2-butanol. Such fermentation methods offer a means of using carbon monoxide from industrial processes which would otherwise be released into the atmosphere and pollute the environment.
    Type: Grant
    Filed: June 10, 2013
    Date of Patent: February 13, 2018
    Assignee: LANZATECH NEW ZEALAND LIMITED
    Inventors: Alexander Paul Mueller, Michael Koepke, Shilpa Nagaraju
  • Patent number: 9796752
    Abstract: It is intended to provide a novel terpenoid derivative that exhibits anti-inflammatory action and a cytoprotective action by activating the Keap1/Nrf2/ARE signaling pathway.
    Type: Grant
    Filed: March 18, 2014
    Date of Patent: October 24, 2017
    Assignee: Daiichi Sankyo Company, Limited
    Inventors: Satoshi Komoriya, Yasuhiro Nakagami, Emiko Hatano, Takashi Ohnuki, Kayoko Masuda, Mayumi Iizuka, Yasunori Ono
  • Patent number: 9738910
    Abstract: At least one isolated microorganism and a fermentation method to convert hydrogen gas, carbon dioxide gas, and/or carbon monoxide gas to a lower alkyl alcohol and/or carboxylic acid and to produce at least 2% by volume of the lower alkyl alcohol or carboxylic acid in an aqueous-based medium.
    Type: Grant
    Filed: August 7, 2013
    Date of Patent: August 22, 2017
    Inventors: Richard Allen Kohn, Seon-Woo Kim
  • Patent number: 9410168
    Abstract: The present disclosure relates to bioengineering approaches for producing biofuel and, in particular, to the use of a C1 metabolizing microorganism reactor system for converting C1 substrates, such as methane or methanol, into biomass and subsequently into biofuels, bioplastics, or the like.
    Type: Grant
    Filed: July 30, 2015
    Date of Patent: August 9, 2016
    Assignee: Calysta, Inc.
    Inventors: Joshua A. Silverman, Sol M. Resnick, Michael Mendez, Renee M. Saville, Sungwon Lee, Luan Nguyen
  • Patent number: 9139853
    Abstract: A non-naturally occurring microbial organism has cyclohexanone pathways that include at least one exogenous nucleic acid encoding a cyclohexanone pathway enzyme. A pathway includes a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on C—C bond), a 2-ketocyclohexane-1-carboxylate decarboxylase and an enzyme selected from a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-1-carboxyl-CoA transferase, and a 2-ketocyclohexane-1-carboxyl-CoA synthetase.
    Type: Grant
    Filed: February 4, 2014
    Date of Patent: September 22, 2015
    Assignee: Genomatica, Inc.
    Inventors: Robin E. Osterhout, Anthony P. Burgard, Mark J. Burk, Priti Pharkya
  • Patent number: 9133443
    Abstract: This invention relates to mutant enzymes with enhanced properties and processes for oxidation of organic compound substrates using such enzymes.
    Type: Grant
    Filed: October 8, 2008
    Date of Patent: September 15, 2015
    Assignee: Isis Innovation Limited
    Inventors: Luet Lok Wong, Stephen Graham Bell, Christopher Whitehouse
  • Publication number: 20150140619
    Abstract: A method comprising a series of selective extraction techniques for the parallel production of biodiesel and isolation of several valuable co-products including an alkenone hydrocarbon mixture of the kerosene/jet fuel range (primarily C10-, C12-, and C17-hydrocarbons) and fucoxanthin, a high-valued carotenoid, from the marine alkenone-producing microalgae Isochrysis.
    Type: Application
    Filed: January 17, 2015
    Publication date: May 21, 2015
    Inventors: Christopher M. Reddy, Gregory W. O'Neil
  • Publication number: 20150133564
    Abstract: Provided are a novel compound, preparing method thereof, and use thereof as inhibitor of histone demethylase. The compound represented by Chemical Formula 1 has activity which inhibits histone demethylase and thus is capable of specifically and effectively inhibit activity of histone demethylase.
    Type: Application
    Filed: April 2, 2014
    Publication date: May 14, 2015
    Applicants: SNU R&DB FOUNDATION, INDUSTRY-ACADEMIC COOPERATION FOUNDATION, YONSEI UNIVERSITY, KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Dong-Chan OH, Seong-Hwan KIM, Jongheon SHIN, Hak Cheol KWON, So Hee KWON
  • Publication number: 20150125912
    Abstract: The invention provides a process for the preparation of hydrocarbons substituted with at least one group containing at least one oxygen atom, comprising the process steps A) reaction of a carbon source comprising at least one selected from CO2 and CO to give acetate and/or ethanol with a first microorganism, B) separating off of the acetate from the first microorganism, C) reaction of the acetate to give a hydrocarbon substituted with at least one group containing at least one oxygen atom with a second microorganism and optionally D) purification of the hydrocarbon substituted with at least one group containing at least one oxygen atom.
    Type: Application
    Filed: May 8, 2013
    Publication date: May 7, 2015
    Applicant: Evonik Industries AG
    Inventors: Thomas Haas, Eva Maria Wittmann
  • Publication number: 20150125903
    Abstract: The present invention provides fungal xylanase and/or xylosidase enzymes suitable for use in saccharification reactions. The present invention provides xylanase and xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase(s) and/or xylosidase(s), as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures. In some embodiments, the xylanase and xylosidase enzyme(s) are M. thermophila enzymes.
    Type: Application
    Filed: January 14, 2015
    Publication date: May 7, 2015
    Inventors: Nicholas John Agard, David Elgart, Jie Yang, Goutami Banerjee, Jeanne Bonomo Benoit, Dipnath Baidyaroy
  • Publication number: 20150111266
    Abstract: The invention provides processes and methods for utilization of carbon dioxide (CO2) in the fermentation of a gaseous substrate comprising hydrogen (H2) and CO2. In particular, the invention allows for the conversion of at least a portion of the CO2 in the gaseous substrate to one or more products, such as ethanol, acetate, and/or 2,3-butanediol.
    Type: Application
    Filed: October 16, 2014
    Publication date: April 23, 2015
    Inventors: Joseph Henry Tizard, Paul Alvin Sechrist
  • Publication number: 20150093796
    Abstract: The present invention provides Clostridium acetobutylicum and an application thereof. A preservation number of the Clostridium acetobutylicum provided in the invention is CGMCC No. 5234. The Clostridium acetobutylicum provided in the present invention can be used for cogeneration of acetone, butanol, ethanol, and 3-hydroxy butanone through fermentation, so as to improve the economic benefit of butanol fermentation. NAD+ coupling and regeneration can be implemented by adding metabolism or growth regulating substances, so as to improve the product yield, and at the same time, the yield of cogeneration products can be flexibly adjusted, so as to cater for the market demand.
    Type: Application
    Filed: March 20, 2012
    Publication date: April 2, 2015
    Inventors: Hanjie Ying, Dong Liu, Yong Chen, Yong Chen, An Li, Tao Zhou, Jinglan Wu, Xiaoqing Lin, Xiaochun Chen, Jingjing Xie, Jianxin Bai
  • Patent number: 8993282
    Abstract: The present invention provides a method for producing a carotenoid, which comprises culturing a carotenoid-producing bacterium in an amino acid-supplemented medium, and collecting the carotenoid from the resulting cultured product, wherein the amino acid is at least one selected, from the group consisting of glutamic acid, aspartic acid, glutamine, asparagine, alanine, glycine, serine, threonine, arginine, tyrosine, proline, phenylalanine and leucine, and salts thereof.
    Type: Grant
    Filed: October 16, 2009
    Date of Patent: March 31, 2015
    Assignee: JX Nippon Oil & Energy Corporation
    Inventors: Kazuaki Hirasawa, Akira Tsubokura, Hiroshi Satou, Tetsuhisa Yata
  • Publication number: 20150087031
    Abstract: The present invention relates to methods of processing lignocellulosic material to obtain hemicellulose sugars, cellulose sugars, lignin, cellulose and other high-value products. Also provided are hemicellulose sugars, cellulose sugars, lignin, cellulose, and other high-value products.
    Type: Application
    Filed: May 3, 2013
    Publication date: March 26, 2015
    Inventors: Robert Jansen, Claire Gregoire, philip Travisano, Lee Madsen, Neta Matis, Yael Har-Tal, Shay Eliahu, James Alan Lawson, Noa Lapidot, Luke Burke, Aharon M. Eyal, Timothy Allen Bauer, Hagit Sade, Paul Mcwilliams, Ziv-Vladimir Belman, Bassem Hallac, Michael Zviely, Yelena Gershinksy, Adam Carden
  • Patent number: 8986961
    Abstract: The invention describes a process for preparing acetone starting from acetyl-coenzyme A comprising process steps A. enzymatic conversion of acetyl-CoA into acetoacetyl-CoA B. enzymatic conversion of acetoacetyl-CoA into acetoacetate and CoA and C. decarboxylation of acetoacetate to acetone and CO2, which is characterized in that the coenzyme A is not transferred in process step B to an acceptor molecule. In addition, process step B is surprisingly catalyzed by enzymes of the classes of acyl-CoA thioesterase, acyl-CoA synthetase or acyl-CoA thiokinase. A completely novel metabolic pathway is concerned, because the enzymatic hydrolysis of acetoacetyl-CoA without simultaneous transfer of CoA to a receptor molecule has never previously been described for any microbial enzyme.
    Type: Grant
    Filed: October 1, 2008
    Date of Patent: March 24, 2015
    Assignee: Evonik Degussa GmbH
    Inventors: Stefan Verseck, Steffen Schaffer, Werner Freitag, Friedrich-Georg Schmidt, Matthias Orschel, Gerda Grund, Wilfried Schmidt, Hubert Johannes Bahl, Ralf-Joerg Fischer, Antje May, Peter Duerre, Simone Lederle
  • Publication number: 20150079649
    Abstract: The present invention relates to a novel valencene synthase, to a nucleic acid encoding such valencene synthase, to a host cell comprising said encoding nucleic acid sequence and to a method for preparing valencene, comprising converting farnesyl diphosphate to valencene in the presence of a valencene synthase according to the invention.
    Type: Application
    Filed: June 21, 2012
    Publication date: March 19, 2015
    Applicant: ISOBIONICS B.V.
    Inventors: Theodorus Sonke, Rene M. De Jong
  • Publication number: 20150082493
    Abstract: The present invention relates to GH61 polypeptide variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: April 26, 2013
    Publication date: March 19, 2015
    Inventors: Janine Lin, Doreen Bohan, Michelle Maranta, Leslie Beresford, Michael Lamsa, Matt Sweeney, Mark Wogulis, Elizabeth Znameroski, Frank Winther Rasmussen
  • Patent number: 8969053
    Abstract: The present invention concerns a method for the production of a biochemical selected among acetol and 1,2-propanediol, 1,3-propanediol, ethylene glycol and 1,4-butanediol comprising culturing a microorganism modified for an improved production of the biochemical selected among acetol and 1,2-propanediol, 1,3-propanediol, ethylene glycol and 1,4-butanediol in an appropriate culture medium and recovery of the desired biochemical which may be further purified wherein the microorganism expresses a YqhD enzyme which catalytic efficiency toward NADPH is increased. The present invention also relates to a mutant YqhD enzyme comprising at least one amino acid residue in the protein sequence of the parent enzyme replaced by a different amino acid residue at the same position wherein the mutant enzyme has retained more than 50% of the YqhD activity of the parent enzyme and the catalytic efficiency toward NADPH of the mutant YqhD is increased as compared with the catalytic efficiency toward NADPH of the parent enzyme.
    Type: Grant
    Filed: July 30, 2010
    Date of Patent: March 3, 2015
    Assignee: Metabolic Explorer
    Inventors: François Voelker, Laurence Dumon-Seignovert, Philippe Soucaille
  • Patent number: 8956835
    Abstract: Methods are provided for producing biodegradable polyhydroxyalkanoates (PHAs) with desired geometry, molecular mass, mechanical and/or physical-chemical properties from glycerol, an inexpensive carbon source and byproduct of the biodiesel industry. Microorganisms capable of converting carbon to PHA can be used to convert biodiesel-glycerol to poly-3-hydroxybutyrate (PHB) or other monomer or copolymer PHAs via fermentation. The microorganisms are cultured in a medium comprising glycerol as a primary carbon source and one or more low molecular mass organic acids as a secondary carbon source. Biomass can be harvested from the culture medium and crude PHA extracted and purified, thereby recovering purified PHA with the desired property. After PHA isolation, a nucleating agent can be added to improve certain physical-chemical properties of the PHA, e.g., crystallization temperature, to enhance performance of the PHA during injection molding.
    Type: Grant
    Filed: November 23, 2011
    Date of Patent: February 17, 2015
    Assignee: Suny Research Foundation
    Inventors: James P. Nakas, Chengjun Zhu, Joseph A. Perrotta, Christopher T. Nomura
  • Publication number: 20150038738
    Abstract: An object of the present invention is to efficiently produce an optically active bicyclic compound. The optically active bicyclic compound is efficiently produced using an enzyme.
    Type: Application
    Filed: October 10, 2014
    Publication date: February 5, 2015
    Applicant: Daiichi Sankyo Company, Limited
    Inventors: Yoshitaka Nakamura, Yukito Furuya, Kousei Shimada, Yoshiyuki Onishi
  • Publication number: 20150031095
    Abstract: The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
    Type: Application
    Filed: October 2, 2014
    Publication date: January 29, 2015
    Inventors: Christopher K. Savile, Vesna Mitchell, Xiyun Zhang, Gjalt W. Huisman
  • Publication number: 20150031534
    Abstract: Provided is a pesticidal Flavobacterium strain and bioactive compositions and metabolites derived therefrom as well as their methods of use for controlling pests.
    Type: Application
    Filed: March 13, 2013
    Publication date: January 29, 2015
    Inventors: Ratnakar Asolkar, Ana-Lucia Cordova-Kreylos, Marja Koivunen, Margarita Rodriguez, Lijuan Xing, Pamela Marrone
  • Patent number: 8940516
    Abstract: The invention concerns a polypeptide which can be isolated from the Brassicaceae family and which has at least the activity of a hydroxynitrile lyase (HNL). The hydroxynitrile lyase of the invention is the first HNL from the Brassicaceae family. The plants (Arabidopsis) from which this enzyme or its gene is isolated is also described as non-cyanogenic. All HNL-containing plants described so far are cyanogenic plants and so it has until now been assumed that only cyanogenic plants contain hydroxynitrile lyases. Surprisingly, it transpires that a polypeptide (AtHNL) of the invention is (R)-selective. The amino acid sequence gives a theoretical molecular weight of 29.2 kDa for the AtHNL subunit. The calculated molecular mass of the protein of approximately 30 kDa can be confirmed by SDS gel electrophoresis.
    Type: Grant
    Filed: November 19, 2012
    Date of Patent: January 27, 2015
    Assignee: Evocatal GmbH
    Inventors: Thorsten Eggert, Jennifer Andexer
  • Patent number: 8927235
    Abstract: The present invention relates to methods of degrading or converting biomass material enriched with hemicellulosic material into fermentable sugars.
    Type: Grant
    Filed: December 6, 2011
    Date of Patent: January 6, 2015
    Assignee: Novozymes A/S
    Inventors: Prashant Iyer, Harry Showmaker, Hui Xu, Kishore Rane
  • Publication number: 20150004656
    Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: September 15, 2014
    Publication date: January 1, 2015
    Inventors: Lan Tang, Ye Liu, Junxin Duan, Yu Zhang, Christian Joergensen, Randall Kramer
  • Publication number: 20150007368
    Abstract: Provided are valencene synthase polypeptides, nucleic acid molecules encoding the valencene synthases, host cells containing the nucleic acids and methods for producing products whose production is catalyzed by the polypeptides. Also provided are methods for producing valencene and nootkatone.
    Type: Application
    Filed: March 11, 2014
    Publication date: January 1, 2015
    Inventors: Dayal Saran, Grace Eunyoung Park
  • Publication number: 20150007369
    Abstract: The present invention relates to GH61 polypeptide variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: November 21, 2012
    Publication date: January 1, 2015
    Inventors: Janine Lin, Doreen Bohan, Michelle Maranta, Leslie Beresford, Michael Lamsa, Bjarne Gram Hansen, Frank Winther Rasmussen, Matt Sweeney, Douglas J. Boyle, III
  • Publication number: 20140370560
    Abstract: The present invention relates to methods and compositions for increasing production of methyl ketones in a genetically modified host cell that overproduces ?-ketoacyl-CoAs through a re-engineered ?-oxidation pathway and overexpresses FadM.
    Type: Application
    Filed: October 26, 2012
    Publication date: December 18, 2014
    Inventors: Harry R. Beller, Ee-Been Goh
  • Publication number: 20140356919
    Abstract: A non-naturally occurring microbial organism has cyclohexanone pathways that include at least one exogenous nucleic acid encoding a cyclohexanone pathway enzyme. A pathway includes a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on C—C bond), a 2-ketocyclohexane-1-carboxylate decarboxylase and an enzyme selected from a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-1-carboxyl-CoA transferase, and a 2-ketocyclohexane-1-carboxyl-CoA synthetase.
    Type: Application
    Filed: February 4, 2014
    Publication date: December 4, 2014
    Inventors: Robin E. Osterhout, Anthony P. Burgard, Mark J. Burk, Priti Pharkya
  • Publication number: 20140349349
    Abstract: The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate, or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.
    Type: Application
    Filed: July 18, 2014
    Publication date: November 27, 2014
    Inventors: MICHAEL DAUNER, Lori Ann Maggio-Hall, Jean-Francois Tomb
  • Publication number: 20140342408
    Abstract: Provided are isolated polypeptides having xylanase activity, catalytic domains and cellulose binding domains, and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.
    Type: Application
    Filed: November 22, 2012
    Publication date: November 20, 2014
    Inventors: Yu Zhang, Lan Tang, Junxin Duan, Ye Liu
  • Publication number: 20140335570
    Abstract: The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: May 8, 2014
    Publication date: November 13, 2014
    Applicant: Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • Patent number: 8883475
    Abstract: The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
    Type: Grant
    Filed: October 23, 2012
    Date of Patent: November 11, 2014
    Assignee: Codexis, Inc.
    Inventors: Christopher Savile, Vesna Mitchell, Xiyun Zhang, Gjalt Huisman