Polyhydric Patents (Class 435/158)
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Patent number: 11952607Abstract: The disclosure provides genetically engineered microorganisms and methods for improved biological production of ethylene glycol and precursors of ethylene glycol. The microorganism of the disclosure produces ethylene glycol or a precursor of ethylene glycol through one or more of 5,10-methylenetetrahydrofolate, oxaloacetate, citrate, malate, and glycine. The disclosure further provides compositions comprising ethylene glycol or polymers of ethylene glycol such as polyethylene terephthalate.Type: GrantFiled: August 5, 2022Date of Patent: April 9, 2024Assignee: LanzaTech, Inc.Inventors: Zachary Robert Cowden, Ching Leang, Michael Koepke, Rasmus Overgaard Jensen, Alexander Paul Mueller
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Patent number: 11441128Abstract: Described herein are fusion proteins including methanol dehydrogenase (MeDH) and at least one other polypeptide such as 3-hexulose-6-phosphate dehydrogenase (HPS) or 6-phospho-3-hexuloisomerase (PHI), such as DHAS synthase or fructose-6-Phosphate aldolase or such as DHA synthase or DHA kinase. In a localized manner, the fusion protein can promote the conversion of methanol to formaldehyde and then to a ketose phosphate such as hexulose 6-phosphate or then to DHA and G3P. When expressed in cells, the fusion proteins can promote methanol uptake and rapid conversion to the ketose phosphate or to the DHA and D3P, which in turn can be used in a pathway for the production of a desired bioproduct. Beneficially, the rapid conversion to the ketose phosphate or to the DHA and G3P can avoid the undesirable accumulation of formaldehyde in the cell.Type: GrantFiled: October 27, 2016Date of Patent: September 13, 2022Assignee: Genomatica, Inc.Inventors: Nelson R. Barton, Jingyi Li, Joseph R. Warner, Priti Pharkya
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Patent number: 11390889Abstract: A method for manufacturing 1,3-propanediol includes culturing, in the presence of a saccharide and formaldehyde to produce 1,3-propanediol, a microorganism having the following genes: (a) a first gene encoding an enzyme that catalyzes an aldol reaction between pyruvic acid and aldehydes; (b) a second gene encoding an enzyme that catalyzes a decarboxylation reaction of ?-keto acids; and (c) a third gene encoding an enzyme that catalyzes a reduction reaction of aldehydes, is provided.Type: GrantFiled: October 30, 2018Date of Patent: July 19, 2022Assignees: Green Earth Institute Co., Ltd., Natural Beauty, LimitedInventors: Keisuke Yamamoto, Atsunari Tsuchisaka, Shuhei Nakane, Toru Nakayashiki
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Patent number: 11371046Abstract: The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or ?-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB).Type: GrantFiled: September 20, 2016Date of Patent: June 28, 2022Assignee: Genomatica, Inc.Inventors: Mark J. Burk, Stephen J. Van Dien, Anthony P. Burgard, Wei Niu
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Patent number: 11254954Abstract: Chemically defined culture medium and culture conditions that allow bacteria to assimilate dinitrogen gas (N2) as a nitrogen source during bio-ethanol production are disclosed herein. Methods of bioethanol production using the chemically defined culture medium and culture conditions are also disclosed.Type: GrantFiled: December 21, 2015Date of Patent: February 22, 2022Assignee: Indiana University Research and Technology CorporationInventors: James McKinlay, Amanda Posto, Timothy Kremer, Breah LaSarre
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Patent number: 11180780Abstract: A non-naturally occurring microbial organism includes a microbial organism having a 1,3-butanediol (1,3-BDO) pathway having at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO.Type: GrantFiled: June 7, 2017Date of Patent: November 23, 2021Assignee: Genomatica, Inc.Inventors: Anthony P. Burgard, Mark J. Burk, Robin E. Osterhout, Priti Pharkya
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Patent number: 10975394Abstract: The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms by engineering a microorganism for increased carbon flux towards mevalonate production in the following enzymatic pathways: (a) citrate synthase, (b) phosphotransacetylase, (c) acetate kinase, (d) lactate dehydrogenase, (e) malic enzyme, and (f) pyruvate dehydrogenase such that one of more of the enzyme activity is modulated. In addition, production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids can be further enhanced by the heterologous expression of the mvaE and mvaS genes (such as, but not limited to, mvaE and mvaS genes from the organisms Listeria grayi DSM 20601, Enterococcus faecium, Enterococcus gallinarum EG2, and Enterococcus casseliflavus).Type: GrantFiled: November 26, 2018Date of Patent: April 13, 2021Inventors: Zachary Q. Beck, Michael C. Miller, Caroline M. Peres, Yuliya A. Primak, Jeff P. Pucci, Derek H. Wells
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Patent number: 10837033Abstract: The invention relates to a method for obtaining at least one organic target product and biogas. The method is characterised in that a) organic substrate is introduced into an anaerobic fermentation process; b) in this anaerobic fermentation process a hydrolysis, acidification and methanation are performed using a mixed culture of bacteria and archaea without spatial separation; c) partial suppression of the anaerobic fermentation is realised, with the result that a part of the organic substrate is not completely decomposed to biogas or methane; d) at least one target product is enriched as an organic metabolite of at least one microorganism in the fermentation process by deliberate incomplete composition of the organic substrate; e) the biogas formed in the anaerobic fermentation process is recovered for further use; and f) at least one organic target product is obtained from the anaerobic fermentation process for further use.Type: GrantFiled: June 6, 2016Date of Patent: November 17, 2020Assignee: VERBIO VEREINIGTE BIOENERGIE AGInventors: Oliver Luedtke, Michael Schlimbach, Enrico Fichter
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Patent number: 10774347Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG) and one or more three-carbon compounds such as acetone, isopropanol or propene. The MEG and one or more three-carbon compounds described herein are useful as starting material for production of other compounds or as end products for industrial and household use. The application further relates to recombinant microorganisms co-expressing a C2 branch pathway and a C3 branch pathway for the production of MEG and one or more three-carbon compounds. Also provided are methods of producing MEG and one or more three-carbon compounds using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally the products MEG and one or more three-carbon compounds.Type: GrantFiled: March 8, 2017Date of Patent: September 15, 2020Assignee: BRASKEM S.A.Inventors: Daniel Johannes Koch, Mateus Schreiner Lopes, Ane Fernanda Beraldi Zeidler, Lucas Pedersen Parizzi
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Patent number: 10759827Abstract: The invention relates to a method for separating and purifying mogroside V by subcritical water desorption technology. The macroporous adsorption resin enriched with mogroside V is subjected to desorption under a subcritical condition of water using water as a solvent, to give an aqueous solution rich in mogroside V. The method not only improves the content of mogroside V in product, but also effectively removes bitter impurities and residual pesticides, greatly improves the taste adaptability of the product, and improves the safety and quality of the product. The method reduces the processing steps and reduces the use of organic solvents in the prior art, and reduces total production costs.Type: GrantFiled: October 10, 2018Date of Patent: September 1, 2020Assignee: Guilin Layn Natural Ingredients Corp.Inventors: Minglian Jiang, Wenguo Yang, Yunfei Song, Yuanyuan Li
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Patent number: 10717997Abstract: The present invention relates to a method for storing gaseous hydrogen, comprising the steps of producing methanoate (formate) through contacting gaseous hydrogen with carbon dioxide in the presence of a hydrogen dependent carbon dioxide reductase (HDCR), and thereby storing of said gaseous hydrogen. The HDCR and/or its complex is preferably derived from Acetobacterium woodii.Type: GrantFiled: June 26, 2018Date of Patent: July 21, 2020Assignee: JOHANN WOLFGANG GOETHE-UNIVERSITÄTInventors: Volker Müller, Kai Schuchmann
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Patent number: 10662136Abstract: A process of isolating 1,4-butanediol (1,4-BDO) from a fermentation broth includes separating a liquid fraction enriched in 1,4-BDO from a solid fraction comprising cells, removing water from said liquid fraction, removing salts from said liquid fraction, and purifying 1,4-BDO. A process for producing 1,4-BDO includes culturing a 1,4-BDO-producing microorganism in a fermentor for a sufficient period of time to produce 1,4-BDO. The 1,4-BDO-producing microorganism includes a microorganism having a 1,4-BDO pathway having one or more exogenous genes encoding a 1,4-BDO pathway enzyme and/or one or more gene disruptions. The process for producing 1,4-BDO further includes isolating 1,4-BDO.Type: GrantFiled: May 9, 2018Date of Patent: May 26, 2020Assignee: Genomatica, Inc.Inventors: Warren Clark, Michael Japs, Mark J. Burk
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Patent number: 10662415Abstract: The present disclosure describes the engineering of microbial cells for fermentative production of (6E)-8-hydroxygeraniol and provides novel engineered microbial cells and cultures, as well as related (6E)-8-hydroxygeraniol production method.Type: GrantFiled: June 24, 2019Date of Patent: May 26, 2020Assignee: Zymergen Inc.Inventors: Cara Ann Tracewell, Steven M. Edgar, Stepan Tymoshenko, Alexander Glennon Shearer
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Patent number: 10626422Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway (MMP) that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,4-butanediol (BDO). Also provided herein are methods for using such an organism to produce BDO.Type: GrantFiled: August 27, 2013Date of Patent: April 21, 2020Assignee: Genomatica, Inc.Inventors: Anthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae
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Patent number: 10584338Abstract: The present invention relates to a novel promoter, a vector comprising the promoter, a microorganism comprising the promoter or the vector, and a method for producing a target product using the microorganism.Type: GrantFiled: March 20, 2017Date of Patent: March 10, 2020Assignee: CJ CHEILJEDANG CORPORATIONInventors: Young Mi Lee, Seung Bin Lee, Seong Bo Kim, Ji Hyun Lee, Seung Hyun Cho, Seung Won Park, Jin Sook Chang
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Patent number: 10525431Abstract: A cold-wall reactor for suspension-bed hydrogenation includes a reactor body including a reaction product outlet, cold hydrogen gas inlet and feed inlet. The reactor body includes a housing, surfacing layer and thermal insulation liner. An inner lining cylinder is fixedly arranged inside the reactor body with an outlet connected with the reaction product outlet. A side wall of the inner lining cylinder and an inner side wall of the reactor body define a cavity serving as a first circulation channel. A second circulation channel is arranged on the inner lining cylinder side wall. The inner lining cylinder communicates with the first circulation channel through the second circulation channel. In suspension-bed hydrogenation, material temperature is more uniform, reaction efficiency is improved, materials coking is reduced, thermal insulation liner issues are prevented, and the temperature of the outer wall of the reactor body is lower than the temperature of the medium.Type: GrantFiled: October 17, 2017Date of Patent: January 7, 2020Assignee: Beijing Huashi United Energy Technology and Development Co., Ltd.Inventors: Lin Li, Lixin Guo, Chuntao Li
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Patent number: 10485255Abstract: A culture product comprising a large quantity of ethyl benzoate and has a more complex and fresher fruity aroma than a chemically synthesized product. The culture product is obtained by culturing a microorganism belonging to the genus Wickerhamomyces in a milk component-containing culture medium.Type: GrantFiled: January 18, 2016Date of Patent: November 26, 2019Assignee: KABUSHIKI KAISHA YAKULT HONSHAInventors: Naoki Izawa, Miyuki Kudo, Toshiro Sone
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Patent number: 10487342Abstract: The invention provides non-naturally occurring microbial organisms containing butadiene or 2,4-pentadienoate pathways comprising at least one exogenous nucleic acid encoding a butadiene or 2,4-pentadienoate pathway enzyme expressed in a sufficient amount to produce butadiene or 2,4-pentadienoate. The organism can further contain a hydrogen synthesis pathway. The invention additionally provides methods of using such microbial organisms to produce butadiene or 2,4-pentadienoate by culturing a non-naturally occurring microbial organism containing butadiene or 2,4-pentadienoate pathways as described herein under conditions and for a sufficient period of time to produce butadiene or 2,4-pentadienoate. Hydrogen can be produced together with the production of butadiene or 2,4-pentadienoate.Type: GrantFiled: July 2, 2015Date of Patent: November 26, 2019Assignee: Genomatica, Inc.Inventors: Priti Pharkya, Anthony P. Burgard, Mark J. Burk
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Patent number: 10385322Abstract: The present invention concerns a method for the production of derivatives of 4-hydroxy-2-ketobutyrate chosen among 1,3-propanediol or 2,4-dihydroxybutyrate by culturing a genetically modified microorganism for the production of the desired derivative of 4-hydroxy-2-ketobutyrate, the microorganism further comprising a gene coding for a mutant glutamate dehydrogenase converting by deamination L-homoserine into 4-hydroxy-2-ketobutyrate. The invention also concerns said genetically modified microorganism.Type: GrantFiled: October 2, 2015Date of Patent: August 20, 2019Assignee: METABOLIC EXPLORERInventors: Zhen Chen, Feng Geng, An-Ping Zeng, Wanda Dischert, Philippe Soucaille
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Patent number: 10208320Abstract: The invention provides a non-naturally occurring microbial organism having a microbial organism having at least one exogenous gene insertion and/or one or more gene disruptions that confer production of primary alcohols. A method for producing long chain alcohols includes culturing these non-naturally occurring microbial organisms.Type: GrantFiled: January 4, 2016Date of Patent: February 19, 2019Assignee: Genomatica, Inc.Inventors: Jun Sun, Anthony P. Burgard, Priti Pharkya
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Patent number: 10173947Abstract: Process for the production of 1,3-butadiene comprising: feeding a mixture (a) comprising 1,3-butanediol and water to an evaporator, said water being present in an amount of greater than or equal to 5% by weight, preferably ranging from 10% by weight to 85% by weight, more preferably ranging from 15% by weight to 30% by weight, relative to the total weight of said mixture (a), to obtain: (b) a gaseous stream comprising 1,3-butanediol exiting from the top of said evaporator; and, optionally, (c) a blowdown stream exiting from the bottom of said evaporator; feeding said gaseous stream (b) to a first reactor containing at least one dehydration catalyst to obtain (d) a stream comprising alkenols, water and, optionally, impurities and/or unreacted 1,3-butanediol, exiting from said first reactor; optionally, feeding said stream (d) to a first purification section to obtain: (e) a stream comprising alkenols, water, and, optionally, impurities; (f) a stream comprising water and, optionally, impurities and/or unreactedType: GrantFiled: December 11, 2015Date of Patent: January 8, 2019Assignee: Versalis S.p.A.Inventors: Nicola Vecchini, Armando Galeotti, Andrea Pisano
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Patent number: 10155937Abstract: The present invention provides various combinations of genetic modifications to a transformed host cell that provide increase conversion of carbon to a chemical product. The present invention also provides methods of fermentation and methods of making various chemical products.Type: GrantFiled: September 19, 2016Date of Patent: December 18, 2018Assignee: CARGILL, INCORPORATEDInventors: Hans Liao, Christopher Patrick Mercogliano, Travis Robert Wolter, Michael Tai Man Louie, Wendy Kathleen Ribble, Tanya E. W. Lipscomb, Eileen Colie Spindler, Michael D. Lynch
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Patent number: 9944954Abstract: Provided herein are glycerol-3-phosphate dehydrogenase (GPD) enzymes with increased KM for NADH and GPD enzymes with substantially the same affinity for NADH and NADPH and/or are feedback inhibited by glycerol-3-phosphate. Also provided herein are recombinant microorganisms comprising a heterologous gene encoding GPD and a deletion or disruption in an endogenous gene encoding GPD. Also provided are recombinant microorganisms comprising a heterologous gene encoding GPD and a butanol biosynthetic pathway. Further provided are methods of producing butanol comprising providing the recombinant microorganisms described herein and contacting the recombinant microorganism with at least one fermentable carbon substrate under conditions wherein butanol is produced.Type: GrantFiled: August 4, 2016Date of Patent: April 17, 2018Assignee: Butamax Advanced Biofuels LLCInventors: Ritu Bhalla, Gopal K. Chotani, Michael Dauner, Mark J. Nelson, Daniel P. O'Keefe, Caroline M. Peres, Jahnavi Chandra Prasad, Jean-Francois Tomb
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Patent number: 9932543Abstract: In one aspect, the present invention is directed to a plant or plant part coated with a composition comprising a bacterial spore and a germinative compound. In another aspect the present invention is directed to a method of enhancing the growth of a plant or plant part comprising coating such plant or plant part with such a composition. In yet another aspect, the present invention is directed to a composition comprising a bacterial spore and a germinative compound where such components are maintained in an inactive form. The present invention also relates to use of the compositions in wastewater treatment, environmental remediation, oil recovery, aquaculture systems, and direct fed microbials.Type: GrantFiled: August 6, 2015Date of Patent: April 3, 2018Assignee: Envera, LLCInventors: Tommie Eugene Hashman, Michael Matheny
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Patent number: 9909150Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol. Also provided herein are methods for using such an organism to produce 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol.Type: GrantFiled: November 4, 2013Date of Patent: March 6, 2018Assignee: Genomatica, Inc.Inventors: Anthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae
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Patent number: 9896701Abstract: The present invention relates to a method for producing 1,3-propanediol using a mutant microorganism lacking a 2,3-butanediol synthetic gene, and more particularly to a mutant microorganism wherein a gene encoding lactate dehydrogenase and a gene encoding an enzyme which is involved in 2,3-butanediol synthesis are deleted in a microorganism having the ability to produce 1,3-propanediol from glycerol and wherein a gene encoding pyruvate decarboxylase and a gene encoding aldehyde dehydrogenase are introduced or amplified, and to a method of promoting the production of 1,3-propanediol while inhibiting the production of 2,3-butanediol by using the mutant microorganism. The use of the glycerol-fermenting mutant microorganism according to the present invention can significantly increase the production of 1,3-propanediol while minimizing the production of 2,3-butanediol.Type: GrantFiled: February 16, 2015Date of Patent: February 20, 2018Assignee: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGYInventors: Chul Ho Kim, Jeong-Woo Seo, Sun-Yeon Heo, Sung Mok Lee, Jang Min Park, Baek Rock Oh, You Ree Jung
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Patent number: 9783832Abstract: The invention relates to a method for producing 2,3-butanediol using improved strains of Raoultella planticola, and to novel mutant strains obtained by random mutagenesis from the bacterial species Raoultella planticola CECT843, that can be used in the industrial production of 2,3-butanediol from glycerol. The invention preferably relates to the Raoultella planticola strains designated IA1 and IIIA3 and deposited in the Spanish Type Culture Collection (CECT) under deposit number CECT8158 (corresponding to the strain designated IA1) and deposit number CECT8159 (corresponding to the strain designated IIIA3). The invention also relates to a method for producing 2,3-butanediol from glycerol by means of a biotechnological process using the novel strains of the invention.Type: GrantFiled: July 22, 2013Date of Patent: October 10, 2017Assignees: Biopolis, S.L., Universidad Complutense de MadridInventors: Antonia Maria Rojas Martinez, Silvia Segarra Manzano, Alejandro Montesinos Paes, Marta Tortajada Serra, Daniel Ramon Vidal, Victoria Eugenia Santos Mazorra, Miguel Ladero Galan, Felix Garcia-Ochoa Soria, Vanessa Ripoll Morales, Alberto Rodriguez Martín
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Patent number: 9708632Abstract: A non-naturally occurring microbial organism includes a microbial organism having a 1,3-butanediol (1,3-BDO) pathway having at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO.Type: GrantFiled: March 30, 2015Date of Patent: July 18, 2017Assignee: Genomatica, Inc.Inventors: Anthony P. Burgard, Mark J. Burk, Robin E. Osterhout, Priti Pharkya
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Patent number: 9657316Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,4-butanediol (BDO). Also provided herein are methods for using such an organism to produce BDO.Type: GrantFiled: August 26, 2013Date of Patent: May 23, 2017Assignee: Genomatica, Inc.Inventors: Anthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae
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Patent number: 9605283Abstract: The invention relates to a modified microorganism for the production of PDO from a carbon substrate wherein the microorganism includes a three-step metabolic pathway including a first step of conversion of 2,4-dihydroxybutyrate (DHB) to obtain 2-oxo-4-hydroxybutyrate (OHB) by an enzyme having 2,4-DHB dehydrogenase activity, a second step of decarboxylation of the OHB to obtain 3-hydroxypropionaldehyde by an enzyme having 2-oxo-4-hydroxybutyrate decarboxylase activity, and a third step of reduction of the obtained 3-hydroxypropionaldehyde to obtain PDO with an enzyme having 3-hydroxypropionaldehyde reductase activity and the genes enabling the microorganism for the synthesis of DHB.Type: GrantFiled: July 10, 2013Date of Patent: March 28, 2017Assignee: INSTITUT NATIONAL DES SCIENCES APPLIQUÉESInventors: Thomas Walther, Jean Marie Francois
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Patent number: 9533931Abstract: A process produces 1,4-butanediol by purifying 1,4-butanediol originated from a fermentation broth, by which process 1,4-butanediol having properties suited as a material of producing a polyester is obtained, which 1,4-butanediol enables to reduce by-production of tetrahydrofuran during the esterification reaction and to reduce the delay in polymerization. The process of producing 1,4-butanediol includes adding an alkaline substance other than an ammonia or an amine to an aqueous 1,4-butanediol-containing solution originated from a fermentation broth; distilling the resulting mixture; and recovering a 1,4-butanediol-containing solution from the vapor flow.Type: GrantFiled: April 9, 2014Date of Patent: January 3, 2017Assignee: Toray Industries, Inc.Inventors: Kenji Kawamura, Izumi Morita, Masateru Ito, Satoshi Sakami, Masataka Makino, Katsushige Yamada, Mark J. Burk, Michael Japs, Warren Clark
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Patent number: 9493746Abstract: Recombinant butyraldehyde dehydrogenases (Blds) with improved production of 1,4-BDO, as well as recombinant microorganisms comprising polynucleotides encoding the recombinant Blds, and methods of producing 1,4-BDO by using the recombinant microorganisms.Type: GrantFiled: October 1, 2014Date of Patent: November 15, 2016Assignees: SAMSUNG ELECTRONICS CO., LTD., AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATIONInventors: Jinhwan Park, Pyungcheon Lee, Jaechan Park, Youngmin Lee, Wooyong Lee, Jinwoo Park, Kwangmyung Cho, Heejin Hwang
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Patent number: 9487803Abstract: The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or ?-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB).Type: GrantFiled: January 22, 2015Date of Patent: November 8, 2016Assignee: Genomatica, Inc.Inventors: Mark J. Burk, Stephen J. Van Dien, Anthony P. Burgard, Wei Niu
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Patent number: 9487451Abstract: The present invention provides novel endophytic yeast strains capable of metabolizing both pentose and hexose sugars. Methods of producing ethanol and xylitol using the novel endophytic yeast are provided herein. Also provided are methods of fixing nitrogen and fertilizing a crop using the novel endophytic yeast strains provided herein.Type: GrantFiled: March 13, 2014Date of Patent: November 8, 2016Assignee: University of Washington Through Its Center for CommercializationInventors: Sharon L. Doty, James Trotter Staley, Michael Su, Azra Vajzovic, Renata Bura, Regina Redman, Zareen Khan
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Patent number: 9434964Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.Type: GrantFiled: January 30, 2012Date of Patent: September 6, 2016Assignee: Genomatica, Inc.Inventors: Stephen J. Van Dien, Anthony P. Burgard, Robert Haselbeck, Catherine J. Pujol-Baxley, Wei Niu, John D. Trawick, Harry Yim, Mark J. Burk, Robin E. Osterhout, Jun Sun
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Patent number: 9410158Abstract: Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications include deletion of non-specific or specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.Type: GrantFiled: September 24, 2013Date of Patent: August 9, 2016Assignee: Cargill IncorporatedInventors: Vineet Rajgarhia, Kari Koivuranta, Merja Penttila, Marja Ilmen, Pirkko Suominen, Aristos Aristidou, Christopher Kenneth Miller, Stacey Olson, Laura Ruohonen
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Patent number: 9387229Abstract: The invention relates to reuterin-producing strains of Lactobacillus brevis. These strains are useful in particular for the treatment or the prevention of conditions resulting from Helicobacter pylori infection.Type: GrantFiled: November 30, 2011Date of Patent: July 12, 2016Assignee: Compagnie Gervais DanoneInventors: Peggy Garault, Gaelle Quere, Raphaelle Bourdet-Sicard
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Patent number: 9353386Abstract: The present invention relates to a host cell having an elevated expression or activity of an enzyme as compared with the parent cell from which it has been derived, said enzyme having lactoyl-CoA reductase activity. Furthermore, provided is a method of producing lactaldehyde and/or 1,2-propanediol, said method comprising culturing said host cell and/or utilizing said enzyme to produce said compound.Type: GrantFiled: June 15, 2012Date of Patent: May 31, 2016Assignee: B.R.A.I.N. Biotechnology Research and Information Network AGInventors: Daniel Koch, Guido Meurer, Jürgen Eck
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Patent number: 9353031Abstract: Hydrothermal digestion of cellulosic biomass solids may be conducted such that a glycol reaction product is formed for subsequent processing. Processing of a glycol reaction product may include a drying operation conducted prior to condensation of the glycol reaction product into higher molecular weight compounds. Methods for digesting cellulosic biomass solids to form a glycol reaction product can comprise: providing cellulosic biomass solids and a slurry catalyst in a hydrothermal digestion unit, the slurry catalyst being capable of activating molecular hydrogen; heating the cellulosic biomass solids in the hydrothermal digestion unit in the presence of the slurry catalyst, a digestion solvent, and molecular hydrogen, thereby forming a liquor phase comprising soluble carbohydrates; and performing a first catalytic reduction reaction on the soluble carbohydrates within the hydrothermal digestion unit, thereby at least partially converting the soluble carbohydrates into a reaction product comprising a glycol.Type: GrantFiled: October 30, 2013Date of Patent: May 31, 2016Assignee: Shell Oil CompanyInventors: Joseph Broun Powell, Kimberly Ann Johnson
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Patent number: 9328360Abstract: A method of producing 1,3-propanediol. The method comprises fermenting a haloalkaliphilic species of Halanaerobium with a source of glycerol into 1,3-propanediol, at a pH of greater than about 10 and at a salt concentration of greater than about 5% w/v. Furthermore, with supplementation of vitamin B12, the yield of 1,3-propanediol to glycerol can be increased.Type: GrantFiled: September 6, 2014Date of Patent: May 3, 2016Assignee: The Curators of the University of MissouriInventors: Melanie Rose Mormile, Daniel William Roush, Dwayne Alexander Elias, Oliver Clifford Sitton
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Patent number: 9328359Abstract: The invention refers to a method of biotransforming a carbohydrate of a raw material into a chemical, by cultivating Lactobacillus diolivorans in the presence of the raw material to produce a chemical substance, and isolating the chemical substance in the purified form, and the use of L. diolivorans in one of a series of biotransformation methods, wherein carbohydrates from at least two different carbohydrate sources of low purity are transformed into chemicals.Type: GrantFiled: November 5, 2012Date of Patent: May 3, 2016Assignee: VOGELBUSCH GMBHInventors: Michael Sauer, Hans Marx, Stefan Pfluegl, Diethard Mattanovich
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Patent number: 9315533Abstract: The invention relates to a method of crystallizing lactose from a lactose-containing liquid comprising the steps of providing a lactose-containing liquid comprising less than 80% by weight total solids, providing an evaporator system that comprises a heat exchanger and an evaporation vessel, the heat exchanger comprising a tube or tubes that define a flowpath having an inlet and an outlet, heating the lactose-containing liquid in the heat exchanger to about 50 to about 90° C. such that the lactose-containing liquid passes along the flowpath by forced circulation or thermo-siphoning, concentrating the lactose-containing liquid in the evaporation vessel, to generate crystallized lactose in the lactose-containing liquid in the evaporator system.Type: GrantFiled: October 10, 2011Date of Patent: April 19, 2016Assignees: FONTERRA CO-OPERATIVE GROUP LIMITED, AUERCON NEW ZEALAND LIMITEDInventors: Anthony James Styles, Paul Stephenson
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Patent number: 9309524Abstract: The present invention relates to methods for obtaining ethanol-producing yeast strains, yeast strains thus produced, and the industrial production of ethanol from said strains. More particularly, the present invention describes in its most general aspect, a method for preparing yeasts from Saccharomyces cerevisiae strains by means of the integration, into the genome of the yeast, of at least one gene encoding xylose isomerase and of at least one gene encoding xylitol dehydrogenase. The strains of the invention are useful for producing ethanol from a medium comprising at least one pentose, preferably xylose or a mixture of xylose and arabinose.Type: GrantFiled: December 2, 2011Date of Patent: April 12, 2016Assignee: LESAFFRE ET COMPAGNIEInventors: Thomas Desfougeres, Georges Pignede
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Patent number: 9303274Abstract: The present invention provides a microorganism belonging to the genus Enterobacter, wherein the microorganism has an ability to assimilate glycerol to produce hydrogen gas and 1,3-propanediol, and wherein the microorganism is capable of assimilating glycerol in the presence of 10 mass % glycerol.Type: GrantFiled: March 30, 2011Date of Patent: April 5, 2016Assignee: SAPPORO BREWERIES LIMITEDInventor: Yukio Okada
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Patent number: 9284581Abstract: A non-naturally occurring microbial organism having a 1,3-butanediol (1,3-BDO) pathway includes at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme or protein expressed in a sufficient amount to produce 1,3-BDO. A method for producing 1,3-BDO that includes culturing the this non-naturally occurring microbial organism under conditions and for a sufficient period of time to produce 1,3-BDO.Type: GrantFiled: August 28, 2012Date of Patent: March 15, 2016Assignee: GENOMATICA, INC.Inventors: Anthony P. Burgard, Mark J. Burk, Priti Pharkya
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Patent number: 9222113Abstract: The invention provides non-naturally occurring microbial organisms comprising 1,4-butanediol (14-BDO) and gamma-butyrolactone (GBL) pathways comprising at least one exogenous nucleic acid encoding a 14-BDO and GBL pathway enzyme expressed in a sufficient amount to produce 14-BDO and GBL. The invention additionally provides methods of using such microbial organisms to produce 14-BDO and GBL.Type: GrantFiled: July 8, 2013Date of Patent: December 29, 2015Assignee: Genomatica, Inc.Inventors: Jun Sun, Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Wei Niu, John D. Trawick, Robert Haselbeck
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Patent number: 9175297Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.Type: GrantFiled: April 17, 2012Date of Patent: November 3, 2015Assignee: Genomatica, Inc.Inventors: Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Jun Sun
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Patent number: 9096860Abstract: A mutant capable of producing 1,4-butanediol and a method of preparing 1,4-butanediol using the same are provided. The mutant microorganism is prepared by introducing and amplifying genes encoding enzymes converting succinate into 4-hydroxybutyrate and 4-hydroxybutyrate into 1,4-butanediol in a microorganism capable of producing succinate. The method includes culturing the mutant in a medium containing carbohydrate and obtaining 1,4-butanediol from the culture. Thus, 1,4-butanediol, which is essential in chemical industry, can be prepared in a biological process.Type: GrantFiled: August 13, 2008Date of Patent: August 4, 2015Assignees: LG CHEM, LTD., KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Si-Jae Park, Sang-Hyun Lee, Sang-Yup Lee, Eun-Jeong Lee
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Patent number: 9062329Abstract: A method for producing arabitol, and more particularly to producing arabitol in a major amount based on a total weight of all polyols produced and in relatively high concentration from a mixture including a carbon source such as glycerol. The method includes in one embodiment utilizing select yeast strains to produce arabitol in high yield while minimizing the amounts of other polyols, using carbon sources such as glycerol as a component in a medium. In a beneficial embodiment, biodiesel byproduct glycerol is used as the substrate for arabitol production.Type: GrantFiled: July 22, 2011Date of Patent: June 23, 2015Assignee: The University of AkronInventor: Lu-Kwang Ju
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Publication number: 20150147786Abstract: A process for mechanical destructuring of starch-based biomass was developed that makes use of a short application of high compression, impact, and shearing forces. The biomass may be destructured using a specific energy input that is less than 40% of the total combustible energy of the biomass. The destructured starch-based biomass, with or without saccharification and/or in-feed glycosyl hydrolase enzymes, may be used in feed applications. The destructured starch-based may saccharified to produce syrups and fermentable sugars, and for production of products including ethanol using a biocatalyst.Type: ApplicationFiled: November 18, 2014Publication date: May 28, 2015Inventors: KATHLEEN A CLARKSON, F Glenn Gallagher, Aaron Perelman, Luis Fernando Romero Millan, Vivek Sharma, Jayarama K. Shetty, Daniel A. Slanac, Paula Johanna Maria Teunissen