Polyhydric Patents (Class 435/158)
  • Patent number: 10385322
    Abstract: The present invention concerns a method for the production of derivatives of 4-hydroxy-2-ketobutyrate chosen among 1,3-propanediol or 2,4-dihydroxybutyrate by culturing a genetically modified microorganism for the production of the desired derivative of 4-hydroxy-2-ketobutyrate, the microorganism further comprising a gene coding for a mutant glutamate dehydrogenase converting by deamination L-homoserine into 4-hydroxy-2-ketobutyrate. The invention also concerns said genetically modified microorganism.
    Type: Grant
    Filed: October 2, 2015
    Date of Patent: August 20, 2019
    Assignee: METABOLIC EXPLORER
    Inventors: Zhen Chen, Feng Geng, An-Ping Zeng, Wanda Dischert, Philippe Soucaille
  • Patent number: 10208320
    Abstract: The invention provides a non-naturally occurring microbial organism having a microbial organism having at least one exogenous gene insertion and/or one or more gene disruptions that confer production of primary alcohols. A method for producing long chain alcohols includes culturing these non-naturally occurring microbial organisms.
    Type: Grant
    Filed: January 4, 2016
    Date of Patent: February 19, 2019
    Assignee: Genomatica, Inc.
    Inventors: Jun Sun, Anthony P. Burgard, Priti Pharkya
  • Patent number: 10173947
    Abstract: Process for the production of 1,3-butadiene comprising: feeding a mixture (a) comprising 1,3-butanediol and water to an evaporator, said water being present in an amount of greater than or equal to 5% by weight, preferably ranging from 10% by weight to 85% by weight, more preferably ranging from 15% by weight to 30% by weight, relative to the total weight of said mixture (a), to obtain: (b) a gaseous stream comprising 1,3-butanediol exiting from the top of said evaporator; and, optionally, (c) a blowdown stream exiting from the bottom of said evaporator; feeding said gaseous stream (b) to a first reactor containing at least one dehydration catalyst to obtain (d) a stream comprising alkenols, water and, optionally, impurities and/or unreacted 1,3-butanediol, exiting from said first reactor; optionally, feeding said stream (d) to a first purification section to obtain: (e) a stream comprising alkenols, water, and, optionally, impurities; (f) a stream comprising water and, optionally, impurities and/or unreacted
    Type: Grant
    Filed: December 11, 2015
    Date of Patent: January 8, 2019
    Assignee: Versalis S.p.A.
    Inventors: Nicola Vecchini, Armando Galeotti, Andrea Pisano
  • Patent number: 10155937
    Abstract: The present invention provides various combinations of genetic modifications to a transformed host cell that provide increase conversion of carbon to a chemical product. The present invention also provides methods of fermentation and methods of making various chemical products.
    Type: Grant
    Filed: September 19, 2016
    Date of Patent: December 18, 2018
    Assignee: CARGILL, INCORPORATED
    Inventors: Hans Liao, Christopher Patrick Mercogliano, Travis Robert Wolter, Michael Tai Man Louie, Wendy Kathleen Ribble, Tanya E. W. Lipscomb, Eileen Colie Spindler, Michael D. Lynch
  • Patent number: 9944954
    Abstract: Provided herein are glycerol-3-phosphate dehydrogenase (GPD) enzymes with increased KM for NADH and GPD enzymes with substantially the same affinity for NADH and NADPH and/or are feedback inhibited by glycerol-3-phosphate. Also provided herein are recombinant microorganisms comprising a heterologous gene encoding GPD and a deletion or disruption in an endogenous gene encoding GPD. Also provided are recombinant microorganisms comprising a heterologous gene encoding GPD and a butanol biosynthetic pathway. Further provided are methods of producing butanol comprising providing the recombinant microorganisms described herein and contacting the recombinant microorganism with at least one fermentable carbon substrate under conditions wherein butanol is produced.
    Type: Grant
    Filed: August 4, 2016
    Date of Patent: April 17, 2018
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Ritu Bhalla, Gopal K. Chotani, Michael Dauner, Mark J. Nelson, Daniel P. O'Keefe, Caroline M. Peres, Jahnavi Chandra Prasad, Jean-Francois Tomb
  • Patent number: 9932543
    Abstract: In one aspect, the present invention is directed to a plant or plant part coated with a composition comprising a bacterial spore and a germinative compound. In another aspect the present invention is directed to a method of enhancing the growth of a plant or plant part comprising coating such plant or plant part with such a composition. In yet another aspect, the present invention is directed to a composition comprising a bacterial spore and a germinative compound where such components are maintained in an inactive form. The present invention also relates to use of the compositions in wastewater treatment, environmental remediation, oil recovery, aquaculture systems, and direct fed microbials.
    Type: Grant
    Filed: August 6, 2015
    Date of Patent: April 3, 2018
    Assignee: Envera, LLC
    Inventors: Tommie Eugene Hashman, Michael Matheny
  • Patent number: 9909150
    Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol. Also provided herein are methods for using such an organism to produce 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol.
    Type: Grant
    Filed: November 4, 2013
    Date of Patent: March 6, 2018
    Assignee: Genomatica, Inc.
    Inventors: Anthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae
  • Patent number: 9896701
    Abstract: The present invention relates to a method for producing 1,3-propanediol using a mutant microorganism lacking a 2,3-butanediol synthetic gene, and more particularly to a mutant microorganism wherein a gene encoding lactate dehydrogenase and a gene encoding an enzyme which is involved in 2,3-butanediol synthesis are deleted in a microorganism having the ability to produce 1,3-propanediol from glycerol and wherein a gene encoding pyruvate decarboxylase and a gene encoding aldehyde dehydrogenase are introduced or amplified, and to a method of promoting the production of 1,3-propanediol while inhibiting the production of 2,3-butanediol by using the mutant microorganism. The use of the glycerol-fermenting mutant microorganism according to the present invention can significantly increase the production of 1,3-propanediol while minimizing the production of 2,3-butanediol.
    Type: Grant
    Filed: February 16, 2015
    Date of Patent: February 20, 2018
    Assignee: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY
    Inventors: Chul Ho Kim, Jeong-Woo Seo, Sun-Yeon Heo, Sung Mok Lee, Jang Min Park, Baek Rock Oh, You Ree Jung
  • Patent number: 9783832
    Abstract: The invention relates to a method for producing 2,3-butanediol using improved strains of Raoultella planticola, and to novel mutant strains obtained by random mutagenesis from the bacterial species Raoultella planticola CECT843, that can be used in the industrial production of 2,3-butanediol from glycerol. The invention preferably relates to the Raoultella planticola strains designated IA1 and IIIA3 and deposited in the Spanish Type Culture Collection (CECT) under deposit number CECT8158 (corresponding to the strain designated IA1) and deposit number CECT8159 (corresponding to the strain designated IIIA3). The invention also relates to a method for producing 2,3-butanediol from glycerol by means of a biotechnological process using the novel strains of the invention.
    Type: Grant
    Filed: July 22, 2013
    Date of Patent: October 10, 2017
    Assignees: Biopolis, S.L., Universidad Complutense de Madrid
    Inventors: Antonia Maria Rojas Martinez, Silvia Segarra Manzano, Alejandro Montesinos Paes, Marta Tortajada Serra, Daniel Ramon Vidal, Victoria Eugenia Santos Mazorra, Miguel Ladero Galan, Felix Garcia-Ochoa Soria, Vanessa Ripoll Morales, Alberto Rodriguez Martín
  • Patent number: 9708632
    Abstract: A non-naturally occurring microbial organism includes a microbial organism having a 1,3-butanediol (1,3-BDO) pathway having at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO.
    Type: Grant
    Filed: March 30, 2015
    Date of Patent: July 18, 2017
    Assignee: Genomatica, Inc.
    Inventors: Anthony P. Burgard, Mark J. Burk, Robin E. Osterhout, Priti Pharkya
  • Patent number: 9657316
    Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,4-butanediol (BDO). Also provided herein are methods for using such an organism to produce BDO.
    Type: Grant
    Filed: August 26, 2013
    Date of Patent: May 23, 2017
    Assignee: Genomatica, Inc.
    Inventors: Anthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae
  • Patent number: 9605283
    Abstract: The invention relates to a modified microorganism for the production of PDO from a carbon substrate wherein the microorganism includes a three-step metabolic pathway including a first step of conversion of 2,4-dihydroxybutyrate (DHB) to obtain 2-oxo-4-hydroxybutyrate (OHB) by an enzyme having 2,4-DHB dehydrogenase activity, a second step of decarboxylation of the OHB to obtain 3-hydroxypropionaldehyde by an enzyme having 2-oxo-4-hydroxybutyrate decarboxylase activity, and a third step of reduction of the obtained 3-hydroxypropionaldehyde to obtain PDO with an enzyme having 3-hydroxypropionaldehyde reductase activity and the genes enabling the microorganism for the synthesis of DHB.
    Type: Grant
    Filed: July 10, 2013
    Date of Patent: March 28, 2017
    Assignee: INSTITUT NATIONAL DES SCIENCES APPLIQUÉES
    Inventors: Thomas Walther, Jean Marie Francois
  • Patent number: 9533931
    Abstract: A process produces 1,4-butanediol by purifying 1,4-butanediol originated from a fermentation broth, by which process 1,4-butanediol having properties suited as a material of producing a polyester is obtained, which 1,4-butanediol enables to reduce by-production of tetrahydrofuran during the esterification reaction and to reduce the delay in polymerization. The process of producing 1,4-butanediol includes adding an alkaline substance other than an ammonia or an amine to an aqueous 1,4-butanediol-containing solution originated from a fermentation broth; distilling the resulting mixture; and recovering a 1,4-butanediol-containing solution from the vapor flow.
    Type: Grant
    Filed: April 9, 2014
    Date of Patent: January 3, 2017
    Assignee: Toray Industries, Inc.
    Inventors: Kenji Kawamura, Izumi Morita, Masateru Ito, Satoshi Sakami, Masataka Makino, Katsushige Yamada, Mark J. Burk, Michael Japs, Warren Clark
  • Patent number: 9493746
    Abstract: Recombinant butyraldehyde dehydrogenases (Blds) with improved production of 1,4-BDO, as well as recombinant microorganisms comprising polynucleotides encoding the recombinant Blds, and methods of producing 1,4-BDO by using the recombinant microorganisms.
    Type: Grant
    Filed: October 1, 2014
    Date of Patent: November 15, 2016
    Assignees: SAMSUNG ELECTRONICS CO., LTD., AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION
    Inventors: Jinhwan Park, Pyungcheon Lee, Jaechan Park, Youngmin Lee, Wooyong Lee, Jinwoo Park, Kwangmyung Cho, Heejin Hwang
  • Patent number: 9487451
    Abstract: The present invention provides novel endophytic yeast strains capable of metabolizing both pentose and hexose sugars. Methods of producing ethanol and xylitol using the novel endophytic yeast are provided herein. Also provided are methods of fixing nitrogen and fertilizing a crop using the novel endophytic yeast strains provided herein.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: November 8, 2016
    Assignee: University of Washington Through Its Center for Commercialization
    Inventors: Sharon L. Doty, James Trotter Staley, Michael Su, Azra Vajzovic, Renata Bura, Regina Redman, Zareen Khan
  • Patent number: 9487803
    Abstract: The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or ?-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB).
    Type: Grant
    Filed: January 22, 2015
    Date of Patent: November 8, 2016
    Assignee: Genomatica, Inc.
    Inventors: Mark J. Burk, Stephen J. Van Dien, Anthony P. Burgard, Wei Niu
  • Patent number: 9434964
    Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.
    Type: Grant
    Filed: January 30, 2012
    Date of Patent: September 6, 2016
    Assignee: Genomatica, Inc.
    Inventors: Stephen J. Van Dien, Anthony P. Burgard, Robert Haselbeck, Catherine J. Pujol-Baxley, Wei Niu, John D. Trawick, Harry Yim, Mark J. Burk, Robin E. Osterhout, Jun Sun
  • Patent number: 9410158
    Abstract: Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications include deletion of non-specific or specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.
    Type: Grant
    Filed: September 24, 2013
    Date of Patent: August 9, 2016
    Assignee: Cargill Incorporated
    Inventors: Vineet Rajgarhia, Kari Koivuranta, Merja Penttila, Marja Ilmen, Pirkko Suominen, Aristos Aristidou, Christopher Kenneth Miller, Stacey Olson, Laura Ruohonen
  • Patent number: 9387229
    Abstract: The invention relates to reuterin-producing strains of Lactobacillus brevis. These strains are useful in particular for the treatment or the prevention of conditions resulting from Helicobacter pylori infection.
    Type: Grant
    Filed: November 30, 2011
    Date of Patent: July 12, 2016
    Assignee: Compagnie Gervais Danone
    Inventors: Peggy Garault, Gaelle Quere, Raphaelle Bourdet-Sicard
  • Patent number: 9353386
    Abstract: The present invention relates to a host cell having an elevated expression or activity of an enzyme as compared with the parent cell from which it has been derived, said enzyme having lactoyl-CoA reductase activity. Furthermore, provided is a method of producing lactaldehyde and/or 1,2-propanediol, said method comprising culturing said host cell and/or utilizing said enzyme to produce said compound.
    Type: Grant
    Filed: June 15, 2012
    Date of Patent: May 31, 2016
    Assignee: B.R.A.I.N. Biotechnology Research and Information Network AG
    Inventors: Daniel Koch, Guido Meurer, Jürgen Eck
  • Patent number: 9353031
    Abstract: Hydrothermal digestion of cellulosic biomass solids may be conducted such that a glycol reaction product is formed for subsequent processing. Processing of a glycol reaction product may include a drying operation conducted prior to condensation of the glycol reaction product into higher molecular weight compounds. Methods for digesting cellulosic biomass solids to form a glycol reaction product can comprise: providing cellulosic biomass solids and a slurry catalyst in a hydrothermal digestion unit, the slurry catalyst being capable of activating molecular hydrogen; heating the cellulosic biomass solids in the hydrothermal digestion unit in the presence of the slurry catalyst, a digestion solvent, and molecular hydrogen, thereby forming a liquor phase comprising soluble carbohydrates; and performing a first catalytic reduction reaction on the soluble carbohydrates within the hydrothermal digestion unit, thereby at least partially converting the soluble carbohydrates into a reaction product comprising a glycol.
    Type: Grant
    Filed: October 30, 2013
    Date of Patent: May 31, 2016
    Assignee: Shell Oil Company
    Inventors: Joseph Broun Powell, Kimberly Ann Johnson
  • Patent number: 9328359
    Abstract: The invention refers to a method of biotransforming a carbohydrate of a raw material into a chemical, by cultivating Lactobacillus diolivorans in the presence of the raw material to produce a chemical substance, and isolating the chemical substance in the purified form, and the use of L. diolivorans in one of a series of biotransformation methods, wherein carbohydrates from at least two different carbohydrate sources of low purity are transformed into chemicals.
    Type: Grant
    Filed: November 5, 2012
    Date of Patent: May 3, 2016
    Assignee: VOGELBUSCH GMBH
    Inventors: Michael Sauer, Hans Marx, Stefan Pfluegl, Diethard Mattanovich
  • Patent number: 9328360
    Abstract: A method of producing 1,3-propanediol. The method comprises fermenting a haloalkaliphilic species of Halanaerobium with a source of glycerol into 1,3-propanediol, at a pH of greater than about 10 and at a salt concentration of greater than about 5% w/v. Furthermore, with supplementation of vitamin B12, the yield of 1,3-propanediol to glycerol can be increased.
    Type: Grant
    Filed: September 6, 2014
    Date of Patent: May 3, 2016
    Assignee: The Curators of the University of Missouri
    Inventors: Melanie Rose Mormile, Daniel William Roush, Dwayne Alexander Elias, Oliver Clifford Sitton
  • Patent number: 9315533
    Abstract: The invention relates to a method of crystallizing lactose from a lactose-containing liquid comprising the steps of providing a lactose-containing liquid comprising less than 80% by weight total solids, providing an evaporator system that comprises a heat exchanger and an evaporation vessel, the heat exchanger comprising a tube or tubes that define a flowpath having an inlet and an outlet, heating the lactose-containing liquid in the heat exchanger to about 50 to about 90° C. such that the lactose-containing liquid passes along the flowpath by forced circulation or thermo-siphoning, concentrating the lactose-containing liquid in the evaporation vessel, to generate crystallized lactose in the lactose-containing liquid in the evaporator system.
    Type: Grant
    Filed: October 10, 2011
    Date of Patent: April 19, 2016
    Assignees: FONTERRA CO-OPERATIVE GROUP LIMITED, AUERCON NEW ZEALAND LIMITED
    Inventors: Anthony James Styles, Paul Stephenson
  • Patent number: 9309524
    Abstract: The present invention relates to methods for obtaining ethanol-producing yeast strains, yeast strains thus produced, and the industrial production of ethanol from said strains. More particularly, the present invention describes in its most general aspect, a method for preparing yeasts from Saccharomyces cerevisiae strains by means of the integration, into the genome of the yeast, of at least one gene encoding xylose isomerase and of at least one gene encoding xylitol dehydrogenase. The strains of the invention are useful for producing ethanol from a medium comprising at least one pentose, preferably xylose or a mixture of xylose and arabinose.
    Type: Grant
    Filed: December 2, 2011
    Date of Patent: April 12, 2016
    Assignee: LESAFFRE ET COMPAGNIE
    Inventors: Thomas Desfougeres, Georges Pignede
  • Patent number: 9303274
    Abstract: The present invention provides a microorganism belonging to the genus Enterobacter, wherein the microorganism has an ability to assimilate glycerol to produce hydrogen gas and 1,3-propanediol, and wherein the microorganism is capable of assimilating glycerol in the presence of 10 mass % glycerol.
    Type: Grant
    Filed: March 30, 2011
    Date of Patent: April 5, 2016
    Assignee: SAPPORO BREWERIES LIMITED
    Inventor: Yukio Okada
  • Patent number: 9284581
    Abstract: A non-naturally occurring microbial organism having a 1,3-butanediol (1,3-BDO) pathway includes at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme or protein expressed in a sufficient amount to produce 1,3-BDO. A method for producing 1,3-BDO that includes culturing the this non-naturally occurring microbial organism under conditions and for a sufficient period of time to produce 1,3-BDO.
    Type: Grant
    Filed: August 28, 2012
    Date of Patent: March 15, 2016
    Assignee: GENOMATICA, INC.
    Inventors: Anthony P. Burgard, Mark J. Burk, Priti Pharkya
  • Patent number: 9222113
    Abstract: The invention provides non-naturally occurring microbial organisms comprising 1,4-butanediol (14-BDO) and gamma-butyrolactone (GBL) pathways comprising at least one exogenous nucleic acid encoding a 14-BDO and GBL pathway enzyme expressed in a sufficient amount to produce 14-BDO and GBL. The invention additionally provides methods of using such microbial organisms to produce 14-BDO and GBL.
    Type: Grant
    Filed: July 8, 2013
    Date of Patent: December 29, 2015
    Assignee: Genomatica, Inc.
    Inventors: Jun Sun, Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Wei Niu, John D. Trawick, Robert Haselbeck
  • Patent number: 9175297
    Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.
    Type: Grant
    Filed: April 17, 2012
    Date of Patent: November 3, 2015
    Assignee: Genomatica, Inc.
    Inventors: Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Jun Sun
  • Patent number: 9096860
    Abstract: A mutant capable of producing 1,4-butanediol and a method of preparing 1,4-butanediol using the same are provided. The mutant microorganism is prepared by introducing and amplifying genes encoding enzymes converting succinate into 4-hydroxybutyrate and 4-hydroxybutyrate into 1,4-butanediol in a microorganism capable of producing succinate. The method includes culturing the mutant in a medium containing carbohydrate and obtaining 1,4-butanediol from the culture. Thus, 1,4-butanediol, which is essential in chemical industry, can be prepared in a biological process.
    Type: Grant
    Filed: August 13, 2008
    Date of Patent: August 4, 2015
    Assignees: LG CHEM, LTD., KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Si-Jae Park, Sang-Hyun Lee, Sang-Yup Lee, Eun-Jeong Lee
  • Patent number: 9062329
    Abstract: A method for producing arabitol, and more particularly to producing arabitol in a major amount based on a total weight of all polyols produced and in relatively high concentration from a mixture including a carbon source such as glycerol. The method includes in one embodiment utilizing select yeast strains to produce arabitol in high yield while minimizing the amounts of other polyols, using carbon sources such as glycerol as a component in a medium. In a beneficial embodiment, biodiesel byproduct glycerol is used as the substrate for arabitol production.
    Type: Grant
    Filed: July 22, 2011
    Date of Patent: June 23, 2015
    Assignee: The University of Akron
    Inventor: Lu-Kwang Ju
  • Publication number: 20150147795
    Abstract: The invention relates to a modified microorganism for the production of PDO from a carbon substrate wherein the microorganism includes a three-step metabolic pathway including a first step of conversion of 2,4-dihydroxybutyrate (DHB) to obtain 2-oxo-4-hydroxybutyrate (OHB) by an enzyme having 2,4-DHB dehydrogenase activity, a second step of decarboxylation of the OHB to obtain 3-hydroxypropionaldehyde by an enzyme having 2-oxo-4-hydroxybutyrate decarboxylase activity, and a third step of reduction of the obtained 3-hydroxypropionaldehyde to obtain PDO with an enzyme having 3-hydroxypropionaldehyde reductase activity and the genes enabling the microorganism for the synthesis of DHB.
    Type: Application
    Filed: July 10, 2013
    Publication date: May 28, 2015
    Inventors: Thomas Walther, Jean Marie Francois
  • Publication number: 20150147786
    Abstract: A process for mechanical destructuring of starch-based biomass was developed that makes use of a short application of high compression, impact, and shearing forces. The biomass may be destructured using a specific energy input that is less than 40% of the total combustible energy of the biomass. The destructured starch-based biomass, with or without saccharification and/or in-feed glycosyl hydrolase enzymes, may be used in feed applications. The destructured starch-based may saccharified to produce syrups and fermentable sugars, and for production of products including ethanol using a biocatalyst.
    Type: Application
    Filed: November 18, 2014
    Publication date: May 28, 2015
    Inventors: KATHLEEN A CLARKSON, F Glenn Gallagher, Aaron Perelman, Luis Fernando Romero Millan, Vivek Sharma, Jayarama K. Shetty, Daniel A. Slanac, Paula Johanna Maria Teunissen
  • Publication number: 20150147794
    Abstract: Disclosed herein is a microorganism capable of producing ethane-1,2-diol from D-xylose, and a method for producing ethane-1,2-diol using the same. More specifically, the present invention relates to an engineered Escherichia coli (E. coli) prepared by knocking out a D-xylose isomerase gene and/or an aldehyde dehydrogenase gene within the genomic DNA of E. coli and transforming an expression vector including a D-xylose dehydrogenase gene into the E. coli, and an efficient method for producing ethane-1,2-diol from D-xylose using the engineered E. coli.
    Type: Application
    Filed: February 5, 2013
    Publication date: May 28, 2015
    Inventors: Wook-Jin Chung, Huaiwei Liu
  • Publication number: 20150148513
    Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate, 1,4-butanediol, or other product pathway and being capable of producing 4-hydroxybutyrate, 1,4-butanediol, or other product, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate, 1,4-butanediol, or other product or related products using the microbial organisms.
    Type: Application
    Filed: June 3, 2013
    Publication date: May 28, 2015
    Applicant: Genomatica, Inc.
    Inventors: Priti Pharkya, Anthony P. Burgard, Stephen J. Van Dien, Robin E. Osterhout, Mark J. Burk, John D. Trawick, Michael P. Kuchinskas, Brian Steer
  • Publication number: 20150140606
    Abstract: A method of producing a sugar liquid from cellulose-containing biomass includes (1) to (4): (1) subjecting a cellulose-containing biomass to a dilute sulfuric acid treatment and thereafter separating the treated cellulose-containing biomass into a dilute sulfuric acid-treated liquid and a cellulose-containing solid content; (2) adding a cellulase to the cellulose-containing solid content to hydrolyze the cellulose and thereafter obtaining a sugar liquid; (3) filtering the dilute sulfuric acid-treated liquid through a nanofiltration membrane at pH 2.5 or lower to thereby separate a sugar concentrated liquid as a retentate and at the same time recover a sulfuric acid aqueous solution as a permeate; and (4) reusing the whole amount or a part of the sulfuric acid aqueous solution obtained in (3) in the dilute sulfuric acid treatment in (1).
    Type: Application
    Filed: June 11, 2013
    Publication date: May 21, 2015
    Inventors: Atsushi Minamino, Hiroyuki Kurihara, Katsushige Yamada
  • Publication number: 20150135369
    Abstract: Provided herein are transgenic plants that include increased expression of a coding region encoding a PAE poly-peptide compared to a control plant. In one embodiment, a transgenic plant includes a phenotype of decreased recalcitrance, increased growth, or the combination thereof. Also provided herein are methods for generating transgenic plants, and methods for using transgenic plants. Examples of methods for using transgenic plants include, for instance, processing a transgenic plant described herein to result in a processed pulp, and exposing a plant material obtained from a plant described herein to conditions suitable for the production of a metabolic product.
    Type: Application
    Filed: March 11, 2013
    Publication date: May 14, 2015
    Inventors: Debra A. Mohnen, Ajaya K. Biswal
  • Publication number: 20150125906
    Abstract: The present invention relates to methods of degrading or converting biomass material enriched with hemicellulosic material into fermentable sugars.
    Type: Application
    Filed: January 14, 2015
    Publication date: May 7, 2015
    Inventors: Prashant Iyer, Harry Showmaker, Hui Xu, Kishore Rane
  • Publication number: 20150125903
    Abstract: The present invention provides fungal xylanase and/or xylosidase enzymes suitable for use in saccharification reactions. The present invention provides xylanase and xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase(s) and/or xylosidase(s), as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures. In some embodiments, the xylanase and xylosidase enzyme(s) are M. thermophila enzymes.
    Type: Application
    Filed: January 14, 2015
    Publication date: May 7, 2015
    Inventors: Nicholas John Agard, David Elgart, Jie Yang, Goutami Banerjee, Jeanne Bonomo Benoit, Dipnath Baidyaroy
  • Patent number: 9017983
    Abstract: A non-naturally occurring microbial organism includes a microbial organism having a 1,3-butanediol (1,3-BDO) pathway having at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO.
    Type: Grant
    Filed: April 30, 2010
    Date of Patent: April 28, 2015
    Assignee: Genomatica, Inc.
    Inventors: Anthony P. Burgard, Mark J. Burk, Robin E. Osterhout, Priti Pharkya
  • Publication number: 20150111262
    Abstract: This document describes biochemical pathways for producing glutaric acid, 5-aminopentanoic acid, 5-hydroxypentanoic acid, cadaverine or 1,5-pentanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C5 backbone substrate such as D-proline.
    Type: Application
    Filed: August 27, 2014
    Publication date: April 23, 2015
    Inventors: Adriana Leonora Botes, Alex Van Eck Conradie
  • Publication number: 20150111266
    Abstract: The invention provides processes and methods for utilization of carbon dioxide (CO2) in the fermentation of a gaseous substrate comprising hydrogen (H2) and CO2. In particular, the invention allows for the conversion of at least a portion of the CO2 in the gaseous substrate to one or more products, such as ethanol, acetate, and/or 2,3-butanediol.
    Type: Application
    Filed: October 16, 2014
    Publication date: April 23, 2015
    Inventors: Joseph Henry Tizard, Paul Alvin Sechrist
  • Publication number: 20150099276
    Abstract: The present invention relates to the use of nucleic acid molecules coding for a bacterial xylose isomerase (XI), preferably coming from Clostridium phytofermentans, for reaction/metabolization, particularly fermentation, of recombinant microorganisms of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol, by means of xylose fermenting yeasts. The present invention further relates to cells, particularly eukaryotic cells, which are transformed utilizing a nucleic acid expression construct which codes for a xylose isomerase, wherein the expression of the nucleic acid expression construct imparts to the cells the capability to directly isomerize xylose into xylulose. Said cells are preferably utilized for reaction/metabolization, particularly fermentation, of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol.
    Type: Application
    Filed: December 16, 2014
    Publication date: April 9, 2015
    Inventors: Dawid BRAT, Eckhard BOLES, Marco KELLER, Beate ROTHER
  • Publication number: 20150093798
    Abstract: A microorganism capable of producing 1,4-butanediol and a method of producing 1,4-butanediol using the same.
    Type: Application
    Filed: September 29, 2014
    Publication date: April 2, 2015
    Inventors: Yukyung Jung, Kwangmyung Cho, Jinhwan Park, Hwayoung Cho
  • Publication number: 20150087031
    Abstract: The present invention relates to methods of processing lignocellulosic material to obtain hemicellulose sugars, cellulose sugars, lignin, cellulose and other high-value products. Also provided are hemicellulose sugars, cellulose sugars, lignin, cellulose, and other high-value products.
    Type: Application
    Filed: May 3, 2013
    Publication date: March 26, 2015
    Inventors: Robert Jansen, Claire Gregoire, philip Travisano, Lee Madsen, Neta Matis, Yael Har-Tal, Shay Eliahu, James Alan Lawson, Noa Lapidot, Luke Burke, Aharon M. Eyal, Timothy Allen Bauer, Hagit Sade, Paul Mcwilliams, Ziv-Vladimir Belman, Bassem Hallac, Michael Zviely, Yelena Gershinksy, Adam Carden
  • Publication number: 20150087028
    Abstract: The invention relates to recombinant expression of variant forms of M. thermophila CBH1a and homologs thereof, having improved thermoactivity, specific activity, and other desirable properties. Also provided are methods for producing ethanol and other valuable organic compounds by combining cellobiohydrolase variants with cellulosic materials.
    Type: Application
    Filed: March 12, 2013
    Publication date: March 26, 2015
    Inventors: Behnaz Behrouzian, Xinkai Xie, Kui Chan, Xiyun Zhang, Vesna Mitchell, Douglas A. Hattendorf
  • Publication number: 20150087037
    Abstract: A methods for altering the metabolite profile of a fermentation, by increasing flux through acetolactate. The methods comprises increasing production of one or more products derived from acetolactate. Further provided is a method for increasing the production of 2,3-butandiol by microbial fermentation of gaseous substrates, the method comprising providing a compound which inhibits one or more enzymes which convert acetolactate to branched chain amino acids to the fermentation. The present invention further provides methods for increasing the production of 2,3-butandiol relative to other fermentation products such as ethanol and acetic acid.
    Type: Application
    Filed: September 22, 2014
    Publication date: March 26, 2015
    Inventors: Kathleen Frances Smart, Alexander Paul Mueller, Michael James Harry Mawdsley, Christophe Daniel Mihalcea
  • Publication number: 20150087038
    Abstract: An object of the present invention is to provide high-quality 1,4BG capable of working out to a raw material of PBT with good color tone, by efficiently removing and refining impurities mixed when producing a biomass-derived 1,4BG on an industrial scale and the present invention relates to a production method of refined 1,4BG, where a crude 1,4BG-containing solution is obtained from refined raw material 1,4BG obtained by removing bacterial cells, salt contents and water from the fermentation culture medium, through a step of removing high-boiling-point components and/or low-boiling-point components by distillation and/or a step of converting an unsaturated compound to a hydride and the target product is obtained as a side stream in a further distillation step.
    Type: Application
    Filed: December 4, 2014
    Publication date: March 26, 2015
    Applicants: MITSUBISHI CHEMICAL CORPORATION, GENOMATICA, INC.
    Inventors: Masaru UTSUNOMIYA, Yusuke IZAWA, Norikazu KONISHI, Kota TANAKA, Shinichiro MATSUZONO, Takayuki SUZUKI, Michael JAPS, Mark BURK, Warren CLARK
  • Patent number: 8986948
    Abstract: The present invention relates to the use of nucleic acid molecules coding for a bacterial xylose isomerase (XI), preferably coming from Clostridium phytofermentans, for reaction/metabolization, particularly fermentation, of recombinant microorganisms of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol, by means of xylose fermenting yeasts. The present invention further relates to cells, particularly eukaryotic cells, which are transformed utilizing a nucleic acid expression construct which codes for a xylose isomerase, wherein the expression of the nucleic acid expression construct imparts to the cells the capability to directly isomerize xylose into xylulose. Said cells are preferably utilized for reaction/metabolization, particularly fermentation, of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol.
    Type: Grant
    Filed: July 1, 2009
    Date of Patent: March 24, 2015
    Assignee: Lesaffre et Compagnie
    Inventors: Dawid Brat, Eckhard Boles, Marco Keller, Beate Wiedemann
  • Publication number: 20150079650
    Abstract: The invention provides methods of increasing the production of fermentation products by increasing flux through a fermentation pathway by optimising enzymatic reactions. In particular, the invention relates to identifying enzymes and/or co-factors involved in metabolic bottlenecks in fermentation pathways, and fermenting a CO-comprising substrate with a recombinant carboxydotrophic Clostridia microorganism adapted to exhibit increased activity of the one or more of said enzymes, or increased availability of the one or more of said co-factors, when compared to a parental microorganism.
    Type: Application
    Filed: June 5, 2014
    Publication date: March 19, 2015
    Inventors: Michael Koepke, Alexander Paul Mueller