Glycerol Patents (Class 435/159)
  • Publication number: 20150140606
    Abstract: A method of producing a sugar liquid from cellulose-containing biomass includes (1) to (4): (1) subjecting a cellulose-containing biomass to a dilute sulfuric acid treatment and thereafter separating the treated cellulose-containing biomass into a dilute sulfuric acid-treated liquid and a cellulose-containing solid content; (2) adding a cellulase to the cellulose-containing solid content to hydrolyze the cellulose and thereafter obtaining a sugar liquid; (3) filtering the dilute sulfuric acid-treated liquid through a nanofiltration membrane at pH 2.5 or lower to thereby separate a sugar concentrated liquid as a retentate and at the same time recover a sulfuric acid aqueous solution as a permeate; and (4) reusing the whole amount or a part of the sulfuric acid aqueous solution obtained in (3) in the dilute sulfuric acid treatment in (1).
    Type: Application
    Filed: June 11, 2013
    Publication date: May 21, 2015
    Inventors: Atsushi Minamino, Hiroyuki Kurihara, Katsushige Yamada
  • Publication number: 20150125906
    Abstract: The present invention relates to methods of degrading or converting biomass material enriched with hemicellulosic material into fermentable sugars.
    Type: Application
    Filed: January 14, 2015
    Publication date: May 7, 2015
    Inventors: Prashant Iyer, Harry Showmaker, Hui Xu, Kishore Rane
  • Publication number: 20150125903
    Abstract: The present invention provides fungal xylanase and/or xylosidase enzymes suitable for use in saccharification reactions. The present invention provides xylanase and xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase(s) and/or xylosidase(s), as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures. In some embodiments, the xylanase and xylosidase enzyme(s) are M. thermophila enzymes.
    Type: Application
    Filed: January 14, 2015
    Publication date: May 7, 2015
    Inventors: Nicholas John Agard, David Elgart, Jie Yang, Goutami Banerjee, Jeanne Bonomo Benoit, Dipnath Baidyaroy
  • Patent number: 9017961
    Abstract: Recombinant bacteria capable of metabolizing sucrose are described. The recombinant bacteria comprise in their genome or on at least one recombinant construct, a novel nucleotide sequence encoding a polypeptide having sucrose transporter activity and a nucleotide sequence encoding a polypeptide having sucrose hydrolase activity. These nucleotide sequences are each operably linked to the same or a different promoter. Recombinant bacteria capable of metabolizing sucrose to produce glycerol and/or glycerol-derived products such as 1,3-propanediol and 3-hydroxypropionic acid are also described.
    Type: Grant
    Filed: March 5, 2012
    Date of Patent: April 28, 2015
    Assignee: E.I. du Pont de Nemours and Company
    Inventors: Kristin Ruebling-Jass, Jean-Francois Tomb, Tina K Van Dyk, Zheng You
  • Publication number: 20150099276
    Abstract: The present invention relates to the use of nucleic acid molecules coding for a bacterial xylose isomerase (XI), preferably coming from Clostridium phytofermentans, for reaction/metabolization, particularly fermentation, of recombinant microorganisms of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol, by means of xylose fermenting yeasts. The present invention further relates to cells, particularly eukaryotic cells, which are transformed utilizing a nucleic acid expression construct which codes for a xylose isomerase, wherein the expression of the nucleic acid expression construct imparts to the cells the capability to directly isomerize xylose into xylulose. Said cells are preferably utilized for reaction/metabolization, particularly fermentation, of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol.
    Type: Application
    Filed: December 16, 2014
    Publication date: April 9, 2015
    Inventors: Dawid BRAT, Eckhard BOLES, Marco KELLER, Beate ROTHER
  • Publication number: 20150087032
    Abstract: A genetically engineered yeast cell that produces a pyruvate-based metabolite from pyruvate, wherein activity of a mitochondrial pyruvate carrier (MPC) is reduced compared to a parent yeast cell and a method of producing the pyruvate-based metabolite using the yeast cell.
    Type: Application
    Filed: July 21, 2014
    Publication date: March 26, 2015
    Inventors: Young-kyoung PARK, Chang-duk Kang, Ji-yoon Song, Ju-young Lee, Seung-hyun Lee, Kwang-myung Cho
  • Publication number: 20150087028
    Abstract: The invention relates to recombinant expression of variant forms of M. thermophila CBH1a and homologs thereof, having improved thermoactivity, specific activity, and other desirable properties. Also provided are methods for producing ethanol and other valuable organic compounds by combining cellobiohydrolase variants with cellulosic materials.
    Type: Application
    Filed: March 12, 2013
    Publication date: March 26, 2015
    Inventors: Behnaz Behrouzian, Xinkai Xie, Kui Chan, Xiyun Zhang, Vesna Mitchell, Douglas A. Hattendorf
  • Patent number: 8986948
    Abstract: The present invention relates to the use of nucleic acid molecules coding for a bacterial xylose isomerase (XI), preferably coming from Clostridium phytofermentans, for reaction/metabolization, particularly fermentation, of recombinant microorganisms of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol, by means of xylose fermenting yeasts. The present invention further relates to cells, particularly eukaryotic cells, which are transformed utilizing a nucleic acid expression construct which codes for a xylose isomerase, wherein the expression of the nucleic acid expression construct imparts to the cells the capability to directly isomerize xylose into xylulose. Said cells are preferably utilized for reaction/metabolization, particularly fermentation, of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol.
    Type: Grant
    Filed: July 1, 2009
    Date of Patent: March 24, 2015
    Assignee: Lesaffre et Compagnie
    Inventors: Dawid Brat, Eckhard Boles, Marco Keller, Beate Wiedemann
  • Publication number: 20150082493
    Abstract: The present invention relates to GH61 polypeptide variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: April 26, 2013
    Publication date: March 19, 2015
    Inventors: Janine Lin, Doreen Bohan, Michelle Maranta, Leslie Beresford, Michael Lamsa, Matt Sweeney, Mark Wogulis, Elizabeth Znameroski, Frank Winther Rasmussen
  • Patent number: 8980578
    Abstract: The present invention provides fungal xylanase and/or beta-xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase and/or beta-xylosidases, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.
    Type: Grant
    Filed: June 10, 2013
    Date of Patent: March 17, 2015
    Assignee: Codexis, Inc.
    Inventors: Ryan Fong, Xiyun Zhang, Chris Noriega, Nicholas Agard, Anupam Gohel, Derek Smith
  • Publication number: 20150064755
    Abstract: Recombinant bacteria capable of metabolizing sucrose are described. The recombinant bacteria comprise in their genome or on at least one recombinant construct, a novel nucleotide sequence encoding a polypeptide having sucrose transporter activity and a nucleotide sequence encoding a polypeptide having sucrose hydrolase activity. These nucleotide sequences are each operably linked to the same or a different promoter. Recombinant bacteria capable of metabolizing sucrose to produce glycerol and/or glycerol-derived products such as 1,3-propanediol and 3-hydroxypropionic acid are also described.
    Type: Application
    Filed: March 5, 2013
    Publication date: March 5, 2015
    Applicant: E I DU PONT DE NEMOURS AND COMPANY
    Inventors: Kristin Ruebling-Jass, Jean-Francois Tomb, Tina K Van Dyk, Zheng You
  • Publication number: 20150050694
    Abstract: A method of sterilizing a separation membrane module using water vapor includes: a liquid supplying step of supplying a liquid having a boiling point of 80° C. or higher at atmospheric pressure to a secondary side of the separation membrane module such that a filling ratio of the liquid in a space surrounded by a filtration portion of a separation membrane is 70% or more, the filtration portion being used for filtration; a liquid sealing step of isolating the secondary side of the separation membrane module such that the filling ratio of the liquid supplied to the secondary side in the liquid supplying step is 70% or more; and a sterilization step of sterilizing the separation membrane module by supplying water vapor to a primary side of the separation membrane module while the secondary side of the separation membrane module is isolated.
    Type: Application
    Filed: March 1, 2013
    Publication date: February 19, 2015
    Applicant: TORAY INDUSTRIES, INC.
    Inventors: Norihiro Takeuchi, Atsushi Kobayashi
  • Publication number: 20150040271
    Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: October 20, 2014
    Publication date: February 5, 2015
    Inventors: Suchindra Maiyuran, Randall Kramer, Paul Harris
  • Publication number: 20150031079
    Abstract: The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: October 13, 2014
    Publication date: January 29, 2015
    Inventor: Nikolaj Spodsberg
  • Publication number: 20150031082
    Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: October 13, 2014
    Publication date: January 29, 2015
    Inventor: Marc Dominique Morant
  • Publication number: 20150031076
    Abstract: The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolized by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.
    Type: Application
    Filed: October 2, 2014
    Publication date: January 29, 2015
    Inventors: Hubertus Johannes Marie OP DEN CAMP, Harry Ramanoedj HARHANGI, Christiaan VAN DER DRIFT, Jacobus Thomas PRONK
  • Publication number: 20150031080
    Abstract: The present invention relates to variants of a parent cellobiohydrolase II. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: October 13, 2014
    Publication date: January 29, 2015
    Inventor: Mark Wogulis
  • Publication number: 20150017683
    Abstract: Scalable biomaterial-based bioreactors are described. In one embodiment, the bioreactor may comprise perforated plates stacked such that the assembled bioreactor has the necessary manifolds and chambers to transport gas and liquids to a biomaterial contained within the bioreactor, and to remove the reaction products. In another embodiment, single use bioreactors are described. Methods of operating the bioreactors are also described.
    Type: Application
    Filed: December 19, 2012
    Publication date: January 15, 2015
    Inventors: Zia Abdullah, Michael L. Dickens, Micah Paul McCreery, Randy L. Jones, Elvin Ray Beach, III, Jon-David S. Sears, Erin Suzanne Schultz, Stephanie Ann Smith, Paul E. George, II
  • Publication number: 20150010943
    Abstract: The present invention relates to genetic modifications in eukaryotic host cells that have been transformed to express a xylose isomerase that confers on the host cell the ability to isomerize xylose to xylulose. These genetic modifications are aimed at improving the efficiency of xylose metabolism and include, e.g., reduction of nonspecific aldose reductase activity, increased xylulose kinase activity and increased flux of the pentose phosphate pathway. The modified host cells of the invention are suitable for the production of a wide variety of fermentation products, including ethanol, in fermentation processes in which a source of xylose or a source of xylose and glucose are used as carbon source.
    Type: Application
    Filed: June 24, 2014
    Publication date: January 8, 2015
    Inventors: Aaron Adriaan WINKLER, Sipko Maarten KUYPER, Wilhelmus Theodorus Antonius Maria DE LAAT, Johannes Pieter VAN DIJKEN, Jacobus Thomas PRONK
  • Publication number: 20150010958
    Abstract: The present invention provides methods for degrading or converting a cellulosic material using an enzyme composition in the presence of a reducing agent. The present invention also provides methods for producing a fermentation product and methods of fermenting a cellulosic material using an enzyme composition in the presence of a reducing agent.
    Type: Application
    Filed: January 28, 2013
    Publication date: January 8, 2015
    Applicants: Novoztmes A/S, Novozymes Inc.
    Inventors: Hongzhi Huang, Yun Wang, Wei Li, Feng Xu, Ani Tejirian
  • Publication number: 20150010970
    Abstract: The present invention provides means for the production of desired end-products of in vitro and/or in vivo bioconversion of biomass-based feed stock substrates, including but not limited to such materials as starch and cellulose. In particularly preferred embodiments, the methods of the present invention do not require gelatinization and/or liquefaction of the substrate.
    Type: Application
    Filed: July 11, 2014
    Publication date: January 8, 2015
    Applicant: Danisco US Inc.
    Inventors: Gopal K. CHOTANI, Manoj KUMAR, Jeff P. PUCCI, Karl J. SANFORD, Jayarama K. SHETTY
  • Publication number: 20150004656
    Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: September 15, 2014
    Publication date: January 1, 2015
    Inventors: Lan Tang, Ye Liu, Junxin Duan, Yu Zhang, Christian Joergensen, Randall Kramer
  • Publication number: 20140377817
    Abstract: The present invention relates to microorganisms and polypeptides for detoxifying aldehydes associated with industrial fermentations. In particular, a heat-stable, NADPH- and iron-dependent alcohol dehydrogenase was cloned from Thermoanaerobacter pseudethanolicus 39E and displayed activity against a number of aldehydes including inhibitory compounds that are produced during the dilute-acid pretreatment process of lignocellulosic biomass before fermentation to biofuels. Methods to use the microorganisms and polypeptides of the invention for improved conversion of bio mass to biofuel are provided as well as use of the enzyme in metabolic engineering strategies for producing longer-chain alcohols from sugars using thermophilic, fermentative microorganisms.
    Type: Application
    Filed: June 20, 2014
    Publication date: December 25, 2014
    Inventors: James G. Elkins, Sonya M. Clarkson
  • Publication number: 20140377813
    Abstract: The invention provides a microbial eukaryotic cell capable of utilizing C5 sugars, in particular xylose. Another objective of the invention is to provide an improved protein sequence to enable eukaryotic cells to degrade C5 sugars. The present invention thus provides protein comprising an amino acid sequence having at least 75% identity, preferably 80% identity, most preferably 90% identity, most highly preferably 95% identity to SEQ ID NO. 2 or SEQ ID NO. 8 and having xylose-isomerase activity in a eukaryotic cell.
    Type: Application
    Filed: February 7, 2013
    Publication date: December 25, 2014
    Inventors: Zdravko Dragovic, Christian Gamauf, Christoph Reisinger, Ulrich Kettling
  • Publication number: 20140370552
    Abstract: The present invention provides endoglucanase 1b (EG1b) variants suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce EG1b variants, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.
    Type: Application
    Filed: December 18, 2012
    Publication date: December 18, 2014
    Inventors: Ee Lui Ang, Ellen D. Eberhard, Xiyun Zhang, Wei Zhang, Jing Tian, Derek J. Smith, Vesna Mitchell
  • Publication number: 20140356915
    Abstract: Processes are described for fractionating lignocellulosic biomass into cellulose, hemicellulose, and lignin, comprising fractionating lignocellulosic biomass in the presence of a solvent for lignin (such as ethanol), a hydrolysis catalyst (such as sulfur dioxide), and water, to produce a liquor containing hemicellulose, cellulose-rich solids, and lignin; hydrolyzing the hemicellulose to produce hemicellulosic monomers; saccharifying the cellulose-rich solids to produce glucose; recovering the hemicellulosic monomers and the glucose, separately or in a combined stream, as fermentable sugars; and fermenting the fermentable sugars to a fermentation product having a higher normal boiling point than water. Process integration of mass and/or energy is disclosed in many specific embodiments. The fermentation product may include an organic acid, an alcohol, a diol, or combinations thereof.
    Type: Application
    Filed: May 23, 2014
    Publication date: December 4, 2014
    Applicant: API Intellectual Property Holdings, LLC
    Inventors: Theodora RETSINA, Vesa PYLKKANEN, Ryan P. O'CONNOR
  • Publication number: 20140342408
    Abstract: Provided are isolated polypeptides having xylanase activity, catalytic domains and cellulose binding domains, and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.
    Type: Application
    Filed: November 22, 2012
    Publication date: November 20, 2014
    Inventors: Yu Zhang, Lan Tang, Junxin Duan, Ye Liu
  • Publication number: 20140331364
    Abstract: Provided are isolated polypeptides having beta-glucosidase activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: December 19, 2012
    Publication date: November 6, 2014
    Inventors: Ye Liu, Junxin Duan, Yu Zhang, Lan Tang
  • Publication number: 20140317786
    Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: November 16, 2012
    Publication date: October 23, 2014
    Inventor: Marc Morant
  • Publication number: 20140315243
    Abstract: The present invention relates to processes for producing fermentation products from starch-containing material, wherein an alpha-amylase, a thermostable protease, and optionally a carbohydrate-source generating enzyme and/or pullulanase, are present and/or added during liquefaction. The invention also relates to compositions suitable for use in a process of the invention.
    Type: Application
    Filed: November 30, 2012
    Publication date: October 23, 2014
    Inventors: Randall Deinhammer, Joyce Craig, Tomoko Matsui, Shinobu Takagi, Suzanne Clark, John Matthews, Anne Glud Hjulmand, Chee-Leong Soong
  • Publication number: 20140308705
    Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: September 20, 2012
    Publication date: October 16, 2014
    Inventors: Marc Morant, Tarana Shaghasi
  • Publication number: 20140308701
    Abstract: The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions.
    Type: Application
    Filed: August 23, 2012
    Publication date: October 16, 2014
    Inventors: Jeffrey Shasky, Amanda Fischer, Suchindra Maiyuran
  • Publication number: 20140302575
    Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol. Also provided herein are methods for using such an organism to produce 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol.
    Type: Application
    Filed: November 4, 2013
    Publication date: October 9, 2014
    Applicant: Genomatica, Inc.
    Inventors: Anthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae
  • Publication number: 20140304859
    Abstract: Provided are isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: December 14, 2012
    Publication date: October 9, 2014
    Applicant: Novozymes Inc.
    Inventors: Ye Liu, Yu Zhang, Junxin Duan, Lan Tang
  • Patent number: 8852903
    Abstract: Recombinant microorganisms having an improved ability to co-metabolize in medium both fructose and glucose are described. The recombinant microorganisms comprise a promoter operably linked to a native or non-native nucleotide sequence that encodes a fucose: H+ symporter polypeptide. The recombinant microorganisms are useful for the production of a variety of products including glycerol and glycerol derivatives such as 1,3-propanediol and 3-hydroxypropionic acid.
    Type: Grant
    Filed: October 18, 2010
    Date of Patent: October 7, 2014
    Assignee: E I du Pont de Nemours and Company
    Inventors: Andrew C. Eliot, Anthony A. Gatenby, John S. Chapman
  • Patent number: 8852901
    Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful intermediates and products, such as energy, fuels, foods or materials. For example, methods are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce an intermediate or product, e.g., by fermentation.
    Type: Grant
    Filed: July 15, 2011
    Date of Patent: October 7, 2014
    Assignee: Xyleco, Inc.
    Inventors: Marshall Medoff, Thomas Craig Masterman, Seul-a Bae, Kelly Wallick
  • Publication number: 20140289905
    Abstract: Provided are isolated polypeptides having xylanase activity, catalytic domains and cellulose binding domains, and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.
    Type: Application
    Filed: November 9, 2012
    Publication date: September 25, 2014
    Applicant: Novozymes Inc.
    Inventors: Yu Zhang, Junxin Duan, Ye Liu, Lan Tang
  • Publication number: 20140287465
    Abstract: The present invention relates to polypeptides having xylanase activity, catalytic domains, and carbohydrate binding domains, and polynucleotides encoding the polypeptides, catalytic domains, and carbohydrate binding domains. The present invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains, and carbohydrate binding domains.
    Type: Application
    Filed: December 18, 2012
    Publication date: September 25, 2014
    Inventors: Nikolaj Spodsberg, Tarana Shaghasi
  • Publication number: 20140287461
    Abstract: A method of producing a sugar liquid using a cellulose-containing biomass as a raw material includes (a) hydrolyzing a cellulose-containing biomass to produce an aqueous sugar solution and (b) filtering the obtained aqueous sugar solution through a reverse osmosis membrane to collect a purified sugar liquid from a feed side, while removing fermentation-inhibiting substances from a permeate side.
    Type: Application
    Filed: May 28, 2014
    Publication date: September 25, 2014
    Applicant: Toray Industries, Inc.
    Inventors: Hiroyuki Kurihara, Atsushi Minamino, Masateru Ito, Hideki Sawai, Masayuki Hanakawa, Shin-ichi Minegishi, Katsushige Yamada
  • Publication number: 20140273105
    Abstract: Lignocellulosic biomass pretreated with ammonia where the concentration of the biomass in the reaction mixture is reduced over time was found to produce more sugars following saccharification, as compared to equivalent biomass pretreated at constant concentration. The concentration of biomass is a solids concentration, which is the percent of dry biomass relative to the total pretreatment reaction mixture on a weight to weight basis.
    Type: Application
    Filed: March 5, 2014
    Publication date: September 18, 2014
    Applicant: E I DU PONT DE NEMOURS AND COMPANY
    Inventors: PATRICIA CHEUNG, BRADLEY CURT FOX, MING WOEI LAU, JOSEPH MICHAEL SELBY, GREGORY PAUL SHANKWITZ, STUART M THOMAS, RYAN ERIC WARNER
  • Publication number: 20140256000
    Abstract: The present invention provides isolated polypeptides having cellulolytic enhancing activity and polynucleotides encoding the polypeptides, catalytic domains, cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.
    Type: Application
    Filed: October 31, 2012
    Publication date: September 11, 2014
    Inventors: Lan Tang, Ye Liu, Yu Zhang, Junxin Duan, Tarana Shaghasi
  • Publication number: 20140248663
    Abstract: Provided are isolated polypeptides having cellobiohydrolase activity, catalytic domains and cellulose binding domains, and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.
    Type: Application
    Filed: November 15, 2012
    Publication date: September 4, 2014
    Inventors: Lan Tang, Junxin Duan, Yu Zhang, Ye Liu
  • Publication number: 20140234906
    Abstract: The present invention relates to alpha-amylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: October 17, 2012
    Publication date: August 21, 2014
    Inventors: Carsten Andersen, Signe Eskildsen Larsen, Esben Peter Friis, Peter Kamp Hansen, Henrik Friis Madsen, Anders Viksoe Nielsen, Pernille Ollendorf Micheelsen
  • Publication number: 20140234914
    Abstract: The present invention relates to recombinant Trichoderma host cells producing Aspergillus fumigatus cellulolytic enzyme compositions and methods of producing and using the compositions.
    Type: Application
    Filed: August 23, 2012
    Publication date: August 21, 2014
    Applicant: NOVOZYMES, INC.
    Inventors: Jeffrey Shasky, Brett McBrayer
  • Patent number: 8802422
    Abstract: The invention provides methods of manufacturing alkanes from triglyceride oils produced through fermentation of oil-bearing microbes. The processes provided herein can utilize a variety of carbohydrate feedstocks including cane bagasse, sugar beet pulp, corn stover, glycerol, corn starch, sorghum, molasses, waste glycerol, and other renewable materials. These processes further comprise hydrotreating, hydrocracking, isomerization, distillation, and other petrochemical processes for use with oil-bearing microbes and products derived therefrom to manufacture fuels. Particular embodiments include the manufacture of ASTM D975 and ASTM D1655 compliant fuels. Genetically engineered microbes provided herein can be used in the manufacture of renewable diesel and renewable jet fuel.
    Type: Grant
    Filed: June 2, 2008
    Date of Patent: August 12, 2014
    Assignee: Solazyme, Inc.
    Inventors: Donald E. Trimbur, Chung-Soon Im, Harrison F. Dillon, Anthony G. Day, Scott Franklin, Anna Coragliotti
  • Publication number: 20140220635
    Abstract: The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: October 17, 2012
    Publication date: August 7, 2014
    Applicant: Novozymes A/S
    Inventors: Carsten Andersen, Signe Larsen, Esben Peter Friis, Pernille Ollendorff Micheelsen, Anders Viksoe Nielsen, Randall Deinhammer, Xinyu Shen
  • Publication number: 20140212932
    Abstract: The present invention relates to enzyme compositions comprising a polypeptide having cellobiohydrolase II activity, a polypeptide having xylanase activity, and one or more cellulolytic proteins and their use in the degradation or conversion of cellulosic material.
    Type: Application
    Filed: April 8, 2014
    Publication date: July 31, 2014
    Applicant: Novozymes, Inc.
    Inventors: Kimberly Brown, Eric Abbate
  • Publication number: 20140206046
    Abstract: Described herein are improved methods of degrading or converting cellulosic material into fermentable sugars using dithionite. Also described are improved methods of fermentation in the presence of dithionite.
    Type: Application
    Filed: September 7, 2012
    Publication date: July 24, 2014
    Inventors: Hui Xu, Ye Chen, Xin Li, Mark Stevens, Zhengfang Kang
  • Publication number: 20140186884
    Abstract: This disclosure relates to novel xylose isomerases and their uses, particularly in fermentation processes that employ xylose-containing media.
    Type: Application
    Filed: July 23, 2013
    Publication date: July 3, 2014
    Applicant: BP Corporation North Americca Inc.
    Inventors: David Neal Nunn, JR., Peter Luginbuhl, Ling Li, Adam Martin Burja, John Peter Flash Bartnek
  • Publication number: 20140178937
    Abstract: A method of producing a sugar liquid with a cellulose-containing biomass as a raw material includes (1) hydrolyzing a cellulose-containing biomass to produce an aqueous sugar solution, and (2) filtering the aqueous sugar solution obtain in (1) through an ultrafiltration membrane having a molecular weight cutoff of 600 to 2,000 to remove a fermentation inhibitor(s) into the permeate side and collect a sugar liquid from the feed side.
    Type: Application
    Filed: July 27, 2012
    Publication date: June 26, 2014
    Applicant: Toray Industries, Inc.
    Inventors: Atsushi Minamino, Hiroyuki Kurihara, Katsushige Yamada