Produced As By-product, Or From Waste, Or From Cellulosic Material Substrate Patents (Class 435/163)
  • Patent number: 10597688
    Abstract: The present invention relates to a method for preparing, from wood-based biomass, a high concentration of fermentable sugar which can be effectively used in culturing various industrial fermented bacteria. According to the method of the present invention, biomass can be extracted by hot water prior to a pre-treatment so as to remove extractible substances such as mineral salts to thus minimize the content of impurities in raw materials for an enzymatic saccharification. The biomass from which substances extractible by hot water are removed is pre-treated in the condition where xylan yield rate is maximized, thus achieving maximum inhibition of the generation of over-decomposed products of sugar.
    Type: Grant
    Filed: December 26, 2013
    Date of Patent: March 24, 2020
    Assignee: Korea Research Institute of Chemical Technology
    Inventors: Ju Hyun Yu, Chan Duck Jung, Seung Hwan Lee, In-Chul Kim, Kyung Sik Hong, In Yong Eom, Jong Geon Jegal, Bong Keun Song
  • Patent number: 10006020
    Abstract: The present invention relates to the use of nucleic acid molecules coding for a bacterial xylose isomerase (XI), preferably coming from Clostridium phytofermentans, for reaction/metabolization, particularly fermentation, of recombinant microorganisms of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol, by means of xylose fermenting yeasts. The present invention further relates to cells, particularly eukaryotic cells, which are transformed utilizing a nucleic acid expression construct which codes for a xylose isomerase, wherein the expression of the nucleic acid expression construct imparts to the cells the capability to directly isomerize xylose into xylulose. Said cells are preferably utilized for reaction/metabolization, particularly fermentation, of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol.
    Type: Grant
    Filed: December 16, 2014
    Date of Patent: June 26, 2018
    Inventors: Dawid Brat, Eckhard Boles, Marco Keller, Beate Rother
  • Patent number: 9885027
    Abstract: The present invention concerns a process for the production of an enzymatic cocktail by submerged culture with a cellulolytic microorganism, comprising two phases: a phase a) for growth of said microorganism in the presence of at least one carbonaceous growth substrate in a closed reactor, said growth phase being carried out with a concentration of carbonaceous growth substrate in the range 10 to 90 g/L; a phase b) for the production of the enzymatic cocktail, in which at least one carbonaceous inducer substrate is supplied, said carbonaceous inducer substrate being at least one solid residue obtained from the step for enzymatic hydrolysis of lignocellulosic materials which have undergone a pre-treatment step, said production phase being carried out with a concentration of carbonaceous production substrate in the range 150 to 400 g/L.
    Type: Grant
    Filed: November 27, 2012
    Date of Patent: February 6, 2018
    Inventors: Fadhel Ben Chaabane, Sylvain Louret
  • Patent number: 9777296
    Abstract: Provided is a method for producing bioproducts, including the steps of: culturing a first microorganism to produce bioalcohol; hydrolyzing the first microorganism; separating the bioalcohol; obtaining wastes from the hydrolyzed bioalcohol fermentation; and inoculating a second microorganism into the wastes from the hydrolyzed bioalcohol fermentation.
    Type: Grant
    Filed: May 14, 2013
    Date of Patent: October 3, 2017
    Inventors: Hyo-Hak Song, Julia Lee, Gi-Wook Choi, Se-Kwon Moon
  • Patent number: 9637434
    Abstract: The invention relates to a method for obtaining ethanol from a carbohydrate-containing raw substrate wherein the ethanol that is produced is separated during fermentation using a carrier gas. The invention further relates to the adsorption of the ethanol from the gas phase to an adsorber, the desorption of the ethanol during a subsequent process step, and to the further concentration of the ethanol.
    Type: Grant
    Filed: December 8, 2010
    Date of Patent: May 2, 2017
    Assignee: SUD-CHEMIE IP GMBH & CO. KG
    Inventors: Michael Zavrel, Michael Kraus, Sandra Hofmann, Ulrich Kettling, Andre Koltermann, Christian Ott, Zdravko Dragovic
  • Patent number: 9611515
    Abstract: The present invention provides methods and compositions suitable for use in the isomerization of xylose to xylulose, as well as methods and compositions suitable for use in the conversion of xylose to xylitol and xylulose, including nucleic acid constructs, recombinant fungal host cells, and related materials.
    Type: Grant
    Filed: November 14, 2013
    Date of Patent: April 4, 2017
    Assignee: Codexis, Inc.
    Inventors: Oscar Alvizo, Mathew G. Miller, Benjamin N. Mijts, Galit Meshulam-Simon, Robert McDaniel
  • Patent number: 9580454
    Abstract: A fractionation process for producing value-added products from a lignocellulosic biomass, comprises: a) mechanically refining the lignocellulosic biomass under mild refining conditions to form a refined biomass pulp with enhanced susceptibility to separation of hemicellulose, cellulose and lignin, and enhanced digestibility of carbohydrates in hydrolysis b) separating hemicellulose and sulfur-free high-quality lignin from cellulose in the refined biomass, and, optionally c) producing various bioproducts from the above said process.
    Type: Grant
    Filed: November 10, 2010
    Date of Patent: February 28, 2017
    Inventors: Zhirun Yuan, Thomas Carl Browne, Xiao Zhang
  • Patent number: 9546386
    Abstract: Provided are microorganisms, e.g., the Saccharomyces yeast, that have been made able to co-ferment xylose sugar-obtained from hydrolyzing plant cellulosic biomass form trees, grasses, straws, etc., with glucose that can be obtained from hydrolyzing either edible feedstocks such as starch, cane sugar, etc. or from hydrolyzing cellulose from various types of non-edible cellulosic biomass. The microorganisms are also capable of expressing an amylase, e.g., glucoamylase, having nonnegligible enzymatic activity, capable of producing glucose from oligo- or polysaccharides obtained by treating soluble starch with ?-amylase. In some embodiments, nucleotidic material is provided comprising genes actively expressing xylose reductase, xylitol dehydrogenase and xylulokinase as well as an active gene expressing glucoamylase. Vectors and other compositions of matter are provided as.
    Type: Grant
    Filed: August 28, 2015
    Date of Patent: January 17, 2017
    Assignee: Tekkware, Inc.
    Inventor: Nancy W. Y. Ho
  • Patent number: 9469837
    Abstract: Disclosed herein are materials and methods for creating and/or isolating variants of yeasts especially variants of Saccharomyces cerevisiae that can grow on sugars other than D-glucose in the presence of amounts of 2-deoxy-glucose and or D-glucose that inhibit most strains of yeast from growing on sugars other than D-glucose. Selection media that can be used to isolate such variants include pentose sugars such as D-xylose, L-glutamine and 2-deoxy-glucose. Mutations in the Grr1 and Red genes in some strains also produce variants that can grow on sugars including the pentose D-xylose in the presence of 2-deoxy-glucose.
    Type: Grant
    Filed: February 25, 2010
    Date of Patent: October 18, 2016
    Assignee: Indiana University Research and Technology Corporation
    Inventors: Mark Goebl, Cary Woods, Ross Cocklin, Josh Heyen
  • Patent number: 9297027
    Abstract: Several mutated strains of Scheffersomyces stipitis are generated by repetitive culturing of the parent strain on two types of concentrated hydrolyzates and with ethanol-challenged xylose-fed continuous culture. Isolates collected from various enriched populations are screened and ranked based on relative xylose uptake rate and ethanol yield. Ranking on hydrolyzates with and without nutritional supplementation is used to identify those isolates with best performance across diverse conditions.
    Type: Grant
    Filed: January 29, 2014
    Date of Patent: March 29, 2016
    Assignees: The United States of America, as represented by the Secretary of Agriculture, Board of Trustees of Michigan State University
    Inventors: Patricia J. Slininger, Stephanie R. Thompson, Venkatesh Balan, Leonardo Da Costa Sousa, Bruce Dale, Maureen A. Shea-Andersh, Bruce S. Dien
  • Patent number: 9206446
    Abstract: A method for producing a microbial growth stimulant (MGS) from a plant biomass is described. In one embodiment, an ammonium hydroxide solution is used to extract a solution of proteins and ammonia from the biomass. Some of the proteins and ammonia are separated from the extracted solution to provide the MGS solution. The removed ammonia can be recycled and the proteins are useful as animal feeds. In one embodiment, the method comprises extracting solubles from pretreated lignocellulosic biomass with a cellulase enzyme-producing growth medium (such T. reesei) in the presence of water and an aqueous extract.
    Type: Grant
    Filed: April 19, 2010
    Date of Patent: December 8, 2015
    Inventors: Ming Woei Lau, Bruce Dale, Venkatesh Balan, Shishir Chundawat
  • Patent number: 9034619
    Abstract: The present invention relates to recombinant bacteria and the uses thereof, particularly for the production of ethanol. The invention also relates to methods for the production of such bacteria, as well as to nucleic acid constructs suitable for such production. The invention specifically relates to bacteria lacking a functional LDH gene and/or containing a recombinant nucleic acid encoding a PDC and ADH. The bacteria of this invention may be produced from any stress-resistant bacteria.
    Type: Grant
    Filed: May 12, 2010
    Date of Patent: May 19, 2015
    Assignee: DEINOVE
    Inventors: Jacques Biton, Esther Gerber
  • Patent number: 9005946
    Abstract: The invention provides variants of the Clostridium thermocellum endoglucanase (CelG) that have improved endoglucanase activity compared to the wild type enzyme. Also provided are related polynucleotides, compositions, vectors, host cells, and methods of use.
    Type: Grant
    Filed: March 10, 2010
    Date of Patent: April 14, 2015
    Assignee: Codexis, Inc.
    Inventors: Jie Yang, Xiyun Zhang, Ish Kumar Dhawan, Andrew Shaw
  • Publication number: 20150093792
    Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: November 24, 2014
    Publication date: April 2, 2015
    Inventors: Sara Landvik, Marc Dominique Morant, Keiichi Ayabe, Guillermo Coward-Kelly
  • Patent number: 8980599
    Abstract: A process for the production of glucose from a pretreated lignocellulosic feedstock is provided. The method comprises enzymatically hydrolyzing the pretreated lignocellulosic feedstock with cellulase enzymes to produce a hydrolyzate slurry comprising glucose and unhydrolyzed cellulose and fermenting the hydrolyzate slurry in a fermentation reaction to produce a fermentation broth comprising alcohol. A process stream is obtained comprising unhydrolyzed cellulose, which is then subjected to a denaturing step, preferably comprising exposing the unhydrolyzed cellulose to elevated temperatures, thereby producing a heat-treated stream comprising the unhydrolyzed cellulose. The heat-treated stream comprising unhydrolyzed cellulose is then further hydrolyzed with cellulase enzymes to hydrolyze the cellulose to glucose.
    Type: Grant
    Filed: July 29, 2008
    Date of Patent: March 17, 2015
    Assignee: Iogen Energy Corporation
    Inventors: Jeffrey S. Tolan, Brian Foody, Stephen Rowland
  • Publication number: 20150050707
    Abstract: A process for the treatment of biomass comprising subjecting biomass to microbial digestion to produce volatile fatty acids and/or solvents followed by wet oxidation to reduce biosolid volume while retaining or increasing the concentration of the volatile fatty acids and/or solvents.
    Type: Application
    Filed: February 27, 2014
    Publication date: February 19, 2015
    Inventors: Daniel James Gapes, Trevor Raymond Stuthridge, Peter James Strong, Robert Jason Lei, Anderson Aggrey
  • Publication number: 20150037857
    Abstract: The present invention relates to methods of making an oil product from plant material that is used to make alcohol (e.g., ethanol) via fermentation. The methods extract oil from the plant material (e.g., corn oil from corn) before exposing to the fermented plant material to distillation temperatures so as to reduce the levels of free fatty acids and/or alcohol esters that can be generated by exposing the oil to distillation temperatures. The present invention also related to oil products made by such methods.
    Type: Application
    Filed: February 21, 2013
    Publication date: February 5, 2015
    Inventor: Steven G. Redford
  • Patent number: 8945352
    Abstract: Methods are provided for changing a molecular structure of a biomass feedstock. The methods include a screw extrusion process.
    Type: Grant
    Filed: May 2, 2011
    Date of Patent: February 3, 2015
    Assignee: Xyleco, Inc.
    Inventor: Marshall Medoff
  • Publication number: 20150024448
    Abstract: Integrated processes are disclosed for the conversion of coal containing mercury compounds, hydrocarbon-containing compounds, sulfur-containing compounds and nitrogen-containing compounds to chemicals in which coal is heated to remove mercury components, pyrolyzed to remove sulfur-containing and nitrogen-containing compounds and then gasified to provide syngas, and syngas is bioconverted to liquid product.
    Type: Application
    Filed: July 15, 2014
    Publication date: January 22, 2015
    Inventors: Mansour Djadali, Norman Louis Balmer
  • Patent number: 8927240
    Abstract: Methods for production of multiple biofuels through thermal fractionation of biomass feedstocks are described.
    Type: Grant
    Filed: November 11, 2011
    Date of Patent: January 6, 2015
    Assignee: The University of Toledo
    Inventors: Balakrishna Maddi, Sridhar Viamajala, Sasidhar Varanasi
  • Patent number: 8927239
    Abstract: A process for improving the yield and efficiency of an ethanol fermentation plant that receives organic fermentable feedstock material, prepares the feedstock for fermentation, ferments the feedstock with yeast to produce ethanol, and produces stillage as a byproduct of ethanol fermentation. The process steps which can be operated independently or in combination, may include, but are not limited to, degrading fatty acids in the fermentable feedstock material prior to fermentation; degrading cellulose and hemicellulose present in the feedstock prior to fermentation; adding a surfactant to the fermentable feedstock; separating a liquid fraction from the stillage; recycling the liquid fraction to be combined with the fermentable feedstock; recovering a solid fraction from the stillage; and introducing at least a portion of the solid fraction to an anaerobic digester to produce methane.
    Type: Grant
    Filed: February 13, 2008
    Date of Patent: January 6, 2015
    Assignee: Water Solutions, Inc.
    Inventors: Stephen D. Allen, Michael R. Rusnack
  • Patent number: 8911979
    Abstract: A method for recovering inorganic salt during processing of a lignocellulosic feedstock is provided. The method comprises pretreating the lignocellulosic feedstock by adding an acid to the feedstock to produce a pretreated lignocellulosic feedstock. A soluble base is then added to the pretreated lignocellulosic feedstock to adjust the pH and produce a neutralized feedstock. The neutralized feedstock is then enzymatically hydrolyzed to produce an enzyme hydrolyzed feedstock and a sugar stream. Inorganic salt is recovered from either a stream obtained from the lignocellulosic feedstock prior to the step of pretreating, a stream obtained from the pretreated lignocellulosic feedstock, a stream obtained from the neutralized feedstock, a stream obtained from the sugar stream, or a combination of these streams. The inorganic salt may be concentrated, clarified, recovered and purified by crystallization, electrodialysis drying, or agglomeration and granulation, and then used as desired, for example as a fertilizer.
    Type: Grant
    Filed: August 8, 2012
    Date of Patent: December 16, 2014
    Assignee: Iogen Energy Corporation
    Inventors: Brian Foody, Jeffrey S. Tolan, Ziyad Rahme, Vijay Anand
  • Patent number: 8877472
    Abstract: Corn or soy plant biomass is electron beam irradiation processed and saccharified to produce sugars. The sugars are then converted to products such as alcohols, organic acids, hydrocarbons, hydrogen, proteins, carbohydrates, fats, oils, lipids, amino acids, vitamins, and mixtures thereof.
    Type: Grant
    Filed: May 24, 2013
    Date of Patent: November 4, 2014
    Assignee: Xyleco, Inc.
    Inventor: Marshall Medoff
  • Patent number: 8865440
    Abstract: The present invention provides isolated or genetically modified strains of microorganisms that display enhanced resistance to acetate as a result of increased expression of a sodium proton antiporter. The present invention also provides methods for producing such microbial strains, as well as related promoter sequences and expression vectors. Further, the present invention provides methods of producing alcohol from biomass materials by using microorganisms with enhanced resistance to acetate.
    Type: Grant
    Filed: November 29, 2012
    Date of Patent: October 21, 2014
    Assignee: UT-Batelle, LLC
    Inventors: Steven D. Brown, Shihui Yang
  • Patent number: 8852918
    Abstract: A biologically pure isolate of a selected bacterium derived from Clostridium autoethanogenum is described which has improved efficiency in the production of ethanol by anaerobic fermentation of substrates comprising carbon monoxide. The bacterium can produce ethanol and acetate at an ethanol to acetate ratio of at least 1.0 and has a productivity of at least 1.2 g of ethanol/l of fermentation broth per day. The bacterium is also characterized in that it has substantially no ability to sporulate.
    Type: Grant
    Filed: June 29, 2012
    Date of Patent: October 7, 2014
    Assignee: Lanzatech New Zealand Limited
    Inventors: Sean Dennis Simpson, Richard Llewellyn Sydney Forster, Phuong Loan Tran, Matthew James Rowe, Ian Linstrand Warner
  • Patent number: 8841091
    Abstract: The present invention relates to polypeptides comprising a carbohydrate-binding module amino acid sequence and an alpha-amylase amino acid sequence as well as to the application of such polypeptides.
    Type: Grant
    Filed: November 2, 2012
    Date of Patent: September 23, 2014
    Assignees: Novozymes Als, Novozymec North America Inc.
    Inventors: Shiro Fukuyama, Tomoko Matsui, Chee Leong Soong, Eric Allain, Anders Vikso Nielsen, Hiroaki Udagawa, Ye Liu, Junxin Duan, Wenping Wu, Lene Nonboe Andersen
  • Publication number: 20140273137
    Abstract: Ethanol losses due to bacterial contamination in fermentation cultures weakens the economics of biofuel production. Lactobacillus species are the predominant contaminant. Bacteriophage lytic enzymes are peptidoglycan hydrolases which degrade Gram positive cell walls when exposed externally and are a novel source of antimicrobials. The streptococcal phage ?SA2 endolysin construct demonstrated strong lytic activity towards 17 of 22 strains of lactobacilli, staphylococci or streptococci maintaining optimal specific activity under fermentation conditions toward L. fermentum substrates. Lactobacillus bacteriophage endolysin constructs LysA, LysA2 and LysgaY showed exolytic activity towards ˜60% of the lactobacilli tested including four L. fermentum isolates from fuel ethanol fermentations. Presence of ethanol (?5%) did not affect lytic activity. Lysins were able to reduce both L. fermentum and L. reuteri contaminants in mock fermentations of corn fiber hydrolysates.
    Type: Application
    Filed: March 15, 2013
    Publication date: September 18, 2014
    Applicant: The United States of America, as represented by the Secretary of Agriculture
    Inventor: The Unites Stated of America, as represented by the Secretary of Agriculture
  • Publication number: 20140272926
    Abstract: High conversion efficiency processes are disclosed for the anaerobic bioconversion of syngas to alcohol by microorganisms having metabolic processes that utilize sulfur in limited amounts. The processes remove hydrogen sulfide from the gas leaving the bioreactor assembly by forming sulfur compounds that are beneficial to the microorganisms. The sulfur compounds can be returned to the bioreactor assembly to meet a portion of microorganism sulfur demand.
    Type: Application
    Filed: March 15, 2013
    Publication date: September 18, 2014
    Applicant: COSKATA, INC.
    Inventor: Richard E. Tobey
  • Patent number: 8818562
    Abstract: A method of modeling a fermentation process comprises providing a first principles model of a fermentation process; determining the concentration of at least one substrate in a fermentation composition at a first time; and predicting the concentration of at least one component of the fermentation composition at a second time using the first principles model, wherein the second time is after the first time.
    Type: Grant
    Filed: June 4, 2011
    Date of Patent: August 26, 2014
    Assignee: Invensys Systems, Inc.
    Inventors: David Bluck, Prashant R. Karbhari, Wen-Jing Lin
  • Patent number: 8809015
    Abstract: Methods and systems for the production of hydrocarbon products, including providing a substrate comprising CO to a bioreactor containing a culture of one or more micro-organisms; and fermenting the culture in the bioreactor to produce one or more hydrocarbon products. The substrate comprising CO is derived from an industrial process selected from the group comprising steam reforming processes, refinery processes, steam cracking processes, and reverse water gas shift processes.
    Type: Grant
    Filed: October 21, 2011
    Date of Patent: August 19, 2014
    Assignee: LanzaTech New Zealand Limited
    Inventors: Michael Anthony Schultz, James Obern, Sean Dennis Simpson
  • Publication number: 20140227753
    Abstract: Integrated processes are disclosed for the anaerobic bioconversion of syngas to alcohol wherein a gas substrate of carbon monoxide, hydrogen and carbon dioxide is in contact with an aqueous menstruum that continuously contacts the gas substrate with said aqueous menstruum to produce alcohol and a depleted gas phase that is continuously withdrawn from the aqueous menstruum; continuously or intermittently and the gas substrate is made up of at least two gases having different compositions to provide an overall gas substrate having a ratio of electrons to carbon atoms in the range of about 5.2:1 to 6.8:1.
    Type: Application
    Filed: February 7, 2014
    Publication date: August 14, 2014
    Inventors: Rathin Datta, Steven G. Calderone, Jianxin Du, Robert Hickey
  • Publication number: 20140206048
    Abstract: The present invention provides compositions and methods for the use of oil-containing materials as feedstocks for the production the bioproducts by biofermentation. In one preferred embodiment, surfactants are not used in compositions and the methods of the invention. In one preferred embodiment the oil-containing feedstocks are the by-products of other industrial processes including microbial, plant and animal oil processing.
    Type: Application
    Filed: January 22, 2014
    Publication date: July 24, 2014
    Applicant: Glycos Biotechnologies, Inc.
    Inventors: Daniel J. Monticello, Ryan Black, Paul Campbell
  • Patent number: 8785155
    Abstract: A method is presented for the production of cellulosic ethanol, acetic acid and derivatives from the extract containing fibers and hemicelluloses after steam cooking of biomass in a host plant. The process is integrated with the host plant process to minimize the effect of loss of heat value from the extracted hemicelluloses and eliminate the need for the waste water treatment plant.
    Type: Grant
    Filed: May 15, 2012
    Date of Patent: July 22, 2014
    Assignee: API Intellectual Property Holdings, LLC
    Inventors: Theodora Retsina, Vesa Pylkkanen, Kimberly Nelson
  • Patent number: 8778668
    Abstract: The present invention provides a modular ethanol production plant constructed of a number of identically sized modules each having a supporting structure main framework to which the components contained in the modules are attached. The plurality of modules includes a fermentation module, a distillation module, a ground grain module, or a sugar source feedstock module, and an optional heating module. Each of the modules are sized to occupy the same approximate space as a standard sized ocean going shipping container. Also, each of the modules has a central walkway including piping and electrical control boxes that are aligned for each connection to the next adjacent module.
    Type: Grant
    Filed: January 13, 2012
    Date of Patent: July 15, 2014
    Inventor: Mark K. Gaalswyk
  • Patent number: 8758517
    Abstract: There is disclosed a process for the hydrolysis of cellulose comprising the sequential steps (a) mixing cellulose with a viscosity below 900 ml/g with an aqueous solution to obtain a liquid, wherein particles comprising cellulose in said liquid have a diameter of maximum 200 nm, wherein the temperature of the aqueous solution is below 35° C., and wherein the pH of the aqueous solution is above 12, (b) subjecting the liquid to at least one of the steps: (i) decreasing the pH of the liquid with at least 1 pH unit and (ii) increasing the temperature by at least 20° C., and (c) hydrolyzing the cellulose. Moreover there is disclose glucose manufactured according to the method and ethanol manufactured from the glucose. Advantages include that the cellulose is hydrolyzed faster and to a greater extent after the treatment. The yield is increased.
    Type: Grant
    Filed: April 16, 2010
    Date of Patent: June 24, 2014
    Assignee: Re:Newcell Lux S.a.r.l
    Inventors: Gunnar Henriksson, Mikael Lindstrom
  • Patent number: 8753838
    Abstract: Provided is a method for producing alcohol or hydrogen gas comprising culturing alcohol-producing microorganisms in a waste fermented solution generated from the bioethanol production process. Glycerol included in the waste fermented solution generated from the bioethanol production process is converted by the microorganisms to butanol under an anaerobic condition. Since the waste fermented solution generated from the bioethanol production process can be utilized as a source of a biofuel, environmental and energy problems can be solved at once.
    Type: Grant
    Filed: February 24, 2011
    Date of Patent: June 17, 2014
    Assignee: Korea Institute of Science and Technology
    Inventors: Youngsoon Um, Byoung-In Sang, Jae Hyung Ahn
  • Patent number: 8722381
    Abstract: Described are variants of alpha (?)-amylases having altered starch hydrolysis profiles. The variants have improved thermostability and increased specific activity, resulting in reduced peak viscosity and altered final viscosity during starch liquefaction. The amylase variants are useful, e.g., in liquefaction and other starch degradation processes.
    Type: Grant
    Filed: February 4, 2009
    Date of Patent: May 13, 2014
    Assignee: Danisco US Inc.
    Inventors: Sandra W. Ramer, Michael J. Pepsin, Andrew Shaw
  • Patent number: 8709769
    Abstract: The invention provides a process for producing alcohol from a cellulosic material, said process comprising the steps of: (i) hydrolyzing said cellulosic material with an aqueous acid to produce a hydrolysate; (ii) extracting acid and water from said hydrolysate with a water-miscible organic extraction solvent to yield (a) a first aqueous acidic solution containing said extraction solvent and (b) a residue containing sugars, —(iii) subjecting said residue to an oligosaccharide cleavage reaction to yield an aqueous solution of fermentable sugars; (iv) fermenting said fermentable sugars and distilling alcohol from the resulting fermented mixture; (v) evaporating said extraction solvent from said first solution to yield (a) a second aqueous acid solution containing no more than 10% wt., preferably no more than 5% wt.
    Type: Grant
    Filed: October 2, 2009
    Date of Patent: April 29, 2014
    Assignee: Weyland AS
    Inventor: Karl Ragnar Weydahl
  • Publication number: 20140106419
    Abstract: The present invention relates to processes and systems for the production of fermentation products such as alcohols. The present invention also provides methods for separating feed stream components for improved biomass processing and productivity.
    Type: Application
    Filed: October 11, 2013
    Publication date: April 17, 2014
    Inventors: Stephane Francois Bazzana, Adam Bernfeld, Keith H. Burlew, Duncan Coffey, James Timothy Cronin, Benjamin Fuchs, John W. Hallam, Adam Jones, David J. Lowe, Brian Michael Roesch, Mathias E. Stolarski, James Gregory Wood, Joseph J. Zaher
  • Patent number: 8697406
    Abstract: Sugars comprising the monosaccharides glucose and fructose, and the disaccharides sucrose and mannose are catalytically converted to ethanol in a sulfate fortified acid medium in the presence of transition metal compounds possessing a degree of symmetry. This is not a fermentation process but is a catalytic chemical process where conversion efficiency is improved by saturating the acidic reaction mixture with inorganic sulfate salts to reduce competitive reactions. Ethanol formed during the reaction is removed by distillation facilitating a continuous process.
    Type: Grant
    Filed: August 16, 2010
    Date of Patent: April 15, 2014
    Inventor: Melvin Keith Carter
  • Patent number: 8697405
    Abstract: The present invention relates to a process for the production of ethanol comprising both gasification and fermentation of feedstocks, and, in particular to a process for the production of ethanol comprising: a) passing a biomass feedstock to a first fermentation step wherein it is subjected to anaerobic fermentation at a pH below 6.0 and at a temperature in the range 20 to 700C to convert the biomass to a solution comprising acetic acid as the predominant product, b) passing a gasifiable feedstock to a gasification step wherein it is subjected to gasification to produce a gaseous mixture comprising carbon monoxide and hydrogen, and c) passing the solution comprising acetic acid from step (a) and the gaseous mixture from step (b) to one or more further fermentation steps wherein they are subject to fermentation to produce ethanol.
    Type: Grant
    Filed: February 19, 2009
    Date of Patent: April 15, 2014
    Assignee: Ineos USA LLC
    Inventors: Peter Simpson Bell, Stephen John Benstead, Neil Turnbull
  • Patent number: 8685684
    Abstract: Disclosed is a preparation method for bio-fuel materials and bio-chemicals comprising the following steps: preparing a medium comprising fermentation waste generated in an alcohol production process; inoculating a first microorganism into the medium; and culturing the medium wherein the first microorganism was inoculated. More specifically, the preparation method for bio-fuel materials and bio-chemicals comprises the following steps: fermenting hexoses from a mixture of pentoses and hexoses to produce an ethanol fermentation broth; separating and purifying the ethanol fermentation broth; preparing a medium comprising the fermentation waste produced in the separation and purification step; inoculating a first microorganism into the medium; and culturing the medium wherein the first microorganism was inoculated.
    Type: Grant
    Filed: September 13, 2010
    Date of Patent: April 1, 2014
    Assignees: GS Caltex Corporation, Changhae Ethanol Co., Ltd.
    Inventors: Julia Lee, Hyo Hak Song, Moon Ho Ueom, Jung Hee Cho, Do Young Seung, Gi-Wook Choi, Se-Kwon Moon, Sung Hoon Park, Yule Kim, Min Hee Han
  • Publication number: 20140080195
    Abstract: A process is provided that is effective for reducing CO2 emissions, enhancing STY and/or increasing cell density. The process allows for utilization of syngas from different sources by controlling concentration levels of CO, CO2 and H2 in syngas provided to the fermentation and by controlling relative concentrations of CO, CO2 and H2 in the syngas provided to the fermentation. The process includes providing syngas to a first fermentation zone and fermenting the syngas. If the first fermentation zone off-gas includes about 4 mole % or more CO, then at least a portion of the first fermentor off-gas is provided to one or more subsequent fermentation zones.
    Type: Application
    Filed: September 4, 2013
    Publication date: March 20, 2014
    Applicant: INEOS BIO SA
    Inventor: Ryan Senaratne
  • Publication number: 20140073025
    Abstract: A system for destruction and recycling of containers (such as bottles, cans and the like), as well as associated liquids, food products or other residue, greatly reduces waste discharge and/or disposal requirements. Packaging and other solids are shredded, ground or otherwise processed, while associated liquids are collected. Evaporation removes water for reuse, thus concentrating sugars and other food materials for utilization as agricultural amendments or stock for bio-product production. Bio-fuels/solvents are generated, typically using an Immobilized Microbe Bioreactor system modified for fuels/solvents production.
    Type: Application
    Filed: September 12, 2013
    Publication date: March 13, 2014
    Inventor: TOMMY MACK DAVIS
  • Patent number: 8663949
    Abstract: This invention relates to the integration of an ammonia production process with a fermentation process to produce products such as alcohols and/or acids in addition to ammonia. In a specific embodiment, a natural gas stream comprising methane is passed to a reforming zone to produce a substrate comprising CO and H2. The substrate is next passed to a bioreactor containing a culture of one or more microorganisms and fermenting the culture to produce one or more fermentation products comprising alcohols and/or acids and an exhaust stream comprising CO2, and H2. The exhaust stream can then be passed to a separation zone to remove at least a portion of the CO2 and produce a purified exhaust stream comprising H2 which is then passed to an ammonia production zone and is used to produce ammonia.
    Type: Grant
    Filed: December 19, 2011
    Date of Patent: March 4, 2014
    Assignee: LanzaTech New Zealand Limited
    Inventor: Michael Anthony Schultz
  • Publication number: 20140057327
    Abstract: The present invention relates to the production of biofuels by utilizing efficient biomass digestion and fermentation by a new thermophilic microorganism generated after fusion of two different bacteria Clostridium thermocellum and C. acetobutylicum and properly mutating the fused bacteria to produce biofuels and other economically important chemicals in a single vessel from lignocellulosic derived renewable biomass. All the necessary biochemical digestions and fermentation are carried out by this single thermophilic microorganism in one single vessel eliminating the need for multiple step digestion and fermentation processes, requiring multiple chambers. It also significantly reduces the rate limiting steps where increasing accumulation of alcohol, butanol and other substances become toxic to the very bacteria that produce these biofuels. The single vessel is incubated at high temperatures (45° C. or above) to eliminate the need for periodic stoppage and restarting of the process.
    Type: Application
    Filed: August 22, 2012
    Publication date: February 27, 2014
    Inventors: Omar Bagasra, Kamal Chowdhury, Verlie A. Tisdale, George E. Miller, III, Rebecca Bullard-Dillard
  • Patent number: 8658407
    Abstract: The present invention relates to compositions and methods for the conversion of lignocellulosic material to fermentable sugars and to products produced therefrom (e.g., ethanol, foodstuffs, etc.). In particular, the invention provides lignocellulose-degrading compositions (e.g., generated via incubation of microbes with lignocellulosic priming feedstock in solid-state fermentation format) and methods of using the same (e.g., in saccharification and/or hydrolysis steps (e.g., on ethanologenic feedstock) and as food or feed additives).
    Type: Grant
    Filed: March 17, 2010
    Date of Patent: February 25, 2014
    Assignee: Alltech, Inc.
    Inventors: Mark P. Lyons, Brian J. Hoskins
  • Patent number: 8652818
    Abstract: A method of producing bioproducts from a feedstock in a system configured to produce ethanol and distillers grains from a fermentation product is disclosed. A system configured to process feedstock into a fermentation product and bioproducts including ethanol and meal is disclosed. A bioproduct produced from a fermentation product produced from a feedstock in a biorefining system is disclosed.
    Type: Grant
    Filed: December 31, 2009
    Date of Patent: February 18, 2014
    Assignee: POET Research, Inc.
    Inventors: John Warren Lawton, Jr., Jason Alan Bootsma, Stephen Michael Lewis
  • Patent number: 8647852
    Abstract: A method of stimulating ethanol production and growth of aquatic plants includes the steps of placing aquatic plants in a cell containing water and creating an oxygenated condition within the cell to initiate an aerobic process. The aquatic plants create and store carbohydrates during the aerobic process. The cell is then covered with a light blocking cover during the anoxic condition to inhibit light from entering the cell. An anoxic condition is created within the cell to initiate an anaerobic process by the aquatic plants. The aquatic plants increase in size and release ethanol into the water by metabolism of stored carbohydrates during the anaerobic process. The ethanol is then sequestered from the water.
    Type: Grant
    Filed: December 1, 2009
    Date of Patent: February 11, 2014
    Inventor: Tony A. Hagen
  • Publication number: 20130344557
    Abstract: Systems and methods for improving ethanol yield are provided. A feedstock is ground suitably fine for use in fermentation. The feedstock may include corn or any other suitable material. In some cases, the feedstock undergoes a fractionation prior to grinding. The ground feedstock may be slurried with water and enzymes to facilitate conversion of the starch in the feedstock to sugars. The slurry may be about 35% solids. After being slurried, an ethanologen may be added. Additionally, the pH of the slurry may be adjusted to between 4.2 and 5.2 to facilitate the priming A primer is added to the slurry. The primer may include any weak acid, and in some embodiments includes acetic acid. Acetic acid, when used as a primer, may be added at a concentration of between 1200 and 3600 parts per million. The slurry is fermented to produce improved yields of ethanol.
    Type: Application
    Filed: March 14, 2012
    Publication date: December 26, 2013
    Applicant: POET RESEARCH, INC.
    Inventors: Stephen M. Lewis, Neelakantam V. Narendranath