Substrate Contains Cellulosic Material Patents (Class 435/165)
  • Patent number: 10858682
    Abstract: The invention relates to a process for the hydrolysis of cellulose containing biomass which comprises a liquefaction step in which a first enzyme or first enzyme composition is added to liquefy at least part of the solids present in the biomass and to keep the viscosity of the cellulose containing biomass below 1000 cP, preferably below 800 cP, more preferably below 600 cP in the liquefaction step; followed by a saccharification step in which a second enzyme composition is added to form oligomeric and/or monomeric sugars; and whereby the first enzyme or first enzyme composition is different from the second enzyme composition; whereby the first enzyme or first enzyme composition comprises an endoglucanase; whereby the second enzyme composition comprises a cellulase; and whereby the first enzyme or first enzyme composition comprises more endoglucanase than the second enzyme composition (expressed in protein wt %).
    Type: Grant
    Filed: November 19, 2018
    Date of Patent: December 8, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Johannes Petrus Smits, Michael Petrus Jozef Berkhout, Bertus Noordam
  • Patent number: 10822625
    Abstract: The present disclosure provides a method for domesticating Saccharomyces cerevisiae which is resistant to fermentation inhibitors, high temperature and high concentration of ethanol. The method comprises a primary domestication and a secondary domestication of the S. cerevisiae, wherein the primary domestication comprises the steps of replacing the fermented mash with fresh liquefied mash and continuing fermentation when the ethanol content in the fermented mash reaches a higher concentration (>12% (v/v)), the replacement is performed for at least 20 times, and the time interval of replacements is 20-30 hours; the secondary domestication comprises the steps of performing high-temperature domestication on the primary domesticated strain by a temperature gradient and replacing the fermented mash for multiple times, wherein the temperature is raised by 0.5° C.-2° C. every 18-24 times of replacement. The domesticated S.
    Type: Grant
    Filed: December 2, 2019
    Date of Patent: November 3, 2020
    Assignees: COFCO (JILIN) BIO-CHEMICAL TECHNOLOGY CO., LTD., COFCO BIO-ENERGY (ZHAODONG) CO., LTD., NUTRITION & HEALTH RESEARCH INSTITUTE, COFCO CORPORATION
    Inventors: Yi Tong, Yi Li, Junqi Zhang, Taibo He, Zexing Wang, Bo Hou, Hui Liu, Fan Li, Lida Wu
  • Patent number: 10815507
    Abstract: The present invention provides, inter alia, a method for combined preparation of saccharides, alcohols (biofuel) and biodiesel using a composition comprising interior hydrophobic or hydrophilic medium encapsulated by a layer comprising cellulose, cellulose derivative material, and/or starch surrounded by a hydrophilic or hydrophobic medium, respectively.
    Type: Grant
    Filed: September 7, 2016
    Date of Patent: October 27, 2020
    Assignee: TECHNION RESEARCH & DEVELOPMENT FOUNDATION LIMITED
    Inventors: Yachin Cohen, Dmitry M. Rein, Lev Vaikhanski, Gilad Alfassi
  • Patent number: 10793537
    Abstract: Disclosed herein are a method of acid saccharification of biomass and a method of polymerization of furan compounds. The method of acid saccharification of biomass comprises recovering pentose-derived furan compounds produced during the acid saccharification process of biomass. The method of acid saccharification of biomass comprises introducing furan compounds into the acid saccharification process of biomass, to produce and recover furan polymers.
    Type: Grant
    Filed: November 14, 2019
    Date of Patent: October 6, 2020
    Assignee: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Jeong-Myeong Ha, Hyemin Yang, Ye Seul Jeong, Jae Wook Choi, Dong Jin Suh, Young Hyun Yoon, Gi Seok Yang, Ung Lee
  • Patent number: 10787527
    Abstract: Disclosed are methods for producing a downstream product from cellulosic material by: a) contacting the cellulosic material with a polymer and a solvent to form a reaction mixture, where the polymer includes acidic monomers and ionic monomers connected to form a polymeric backbone, and each of the ionic monomers independently comprises at least one nitrogen-containing cationic group or at least one phosphorous-containing cationic group; b) degrading the cellulosic material in the reaction mixture to produce a liquid phase and a solid phase, where the liquid phrase includes one or more sugars, and where the solid phase includes residual cellulosic material; and c) using the one or more sugars to produce the downstream product.
    Type: Grant
    Filed: October 30, 2018
    Date of Patent: September 29, 2020
    Assignee: Cadena Bio, Inc.
    Inventors: John M. Geremia, Brian M. Baynes, Ashish Dhawan
  • Patent number: 10767196
    Abstract: The present invention provides for the manipulation of cofactor usage in a recombinant host cell to increase the formation of desirable products. In some embodiments, the invention provides for a recombinant microorganism comprising a mutation in one or more native enzymes such that their cofactor specificity is altered in such a way that overall cofactor usage in the cell is balanced for a specified pathway and there is an increase in a specific product formation within the cell. In some embodiments, endogenous enzymes are replaced by enzymes with an alternate cofactor specificity from a different species.
    Type: Grant
    Filed: November 30, 2012
    Date of Patent: September 8, 2020
    Assignees: Enchi Corporation, Dartmouth College, UT-Battelle, LLC
    Inventors: Jonathan Lo, Adam M. Guss, Johannes P. Van Dijken, Arthur J. Shaw, IV, Daniel G. Olson, Christopher D. Herring, D. Aaron Argyros, Nicky Caiazza
  • Patent number: 10738317
    Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
    Type: Grant
    Filed: May 3, 2018
    Date of Patent: August 11, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Jacobus Thomas Pronk, Antonius Jeroen Adriaan Van Maris, Victor Gabriel Guadalupe Medina
  • Patent number: 10711286
    Abstract: The invention relates to an integrated process for alcohol production and organic acid production from lignocellulosic material.
    Type: Grant
    Filed: March 27, 2019
    Date of Patent: July 14, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventor: Johannes Petrus Smits
  • Patent number: 10619172
    Abstract: Disclosed are methods for engineering bacteria, for example, Thermoanaerobacterium saccharolyticum, that convert biomass to ethanol at high yield by deleting a single gene. Deletion of subunit A or subunit B of the hfs hydrogenase, but not deletion of subunit C or subunit D, results in an increase in ethanol yield.
    Type: Grant
    Filed: July 21, 2017
    Date of Patent: April 14, 2020
    Assignee: THE TRUSTEES OF DARTMOUTH COLLEGE
    Inventors: Aysenur Eminoglu, Daniel G. Olson, Lee R. Lynd
  • Patent number: 10604777
    Abstract: The present disclosure relates to a method for obtaining sugar and lignin fractions from lignocellulosic materials and to a method for producing fermentation products using the sugars obtained from the lignocellulose. The present disclosure relates to a method for improving the sugar yield in the enzymatic hydrolysate by introducing a pre-treatment step of hydrothermal hydrolysis and an alkaline delignification step.
    Type: Grant
    Filed: December 11, 2014
    Date of Patent: March 31, 2020
    Assignee: NESTE OYJ
    Inventors: Heidi Vainio, Ville Pihlajaniemi, Mika Sipponen, Ossi Pastinen, Ilkka Lehtomäki, Simo Laakso, Olavi Myllymäki, Perttu Koskinen
  • Patent number: 10597601
    Abstract: A method is disclosed for recovering lipids from microbial biomass, and for producing lipids. In the method, an aqueous suspension includes fermentation broth containing oleaginous yeast biomass which is subjected to hydrothermal treatment and lipids are recovered from the biomass by solvent extraction.
    Type: Grant
    Filed: June 16, 2015
    Date of Patent: March 24, 2020
    Assignee: NESTE OYJ
    Inventors: Arto Heiska, Annika Malm, Jukka-Pekka Pasanen
  • Patent number: 10465214
    Abstract: Producing a resin from an organic waste product includes assessing a weight percent of a first volatile fatty acid and a weight percent of a second volatile fatty acid in a liquid mixture having volatile fatty acids from the organic waste product. The weight percent of the volatile fatty acids is based on the total weight of the carboxylic acids in the liquid mixture, the total weight of volatile fatty acids in the liquid mixture, or the total weight of lactic acid and volatile fatty acids in the mixture. A ratio of the weight percent of the first volatile fatty acid to the weight percent of the second volatile fatty acid in the liquid mixture is adjusted to yield a modified liquid mixture. The modified liquid is combined with polyhydroxyalkanoate-producing bacteria to yield a polyhydroxyalkanoate copolymer; and the polyhydroxyalkanoate copolymer is extracted from the polyhydroxyalkanoate-producing bacteria.
    Type: Grant
    Filed: November 20, 2015
    Date of Patent: November 5, 2019
    Assignee: Full Cycle Bioplastics LLC
    Inventors: Jeff H. Anderson, Dane H. Anderson
  • Patent number: 10316341
    Abstract: The present invention is directed to a process for self-sufficient hydrolysis of lignocellulosic material. In an additional aspect, the present invention is directed to a process for the production of an organic product and the organic product produced according to this process.
    Type: Grant
    Filed: May 20, 2015
    Date of Patent: June 11, 2019
    Assignee: Clariant International Ltd.
    Inventors: Michael Zavrel, Danielle Dennewald, Joerg Bartuch, Henning Marckmann
  • Patent number: 10316340
    Abstract: A glucose production method is characterized in that glucose is produced from organisms that hold at least a disaccharide inside the body, which organisms utilize a cellulose source as food or for nutrients, particularly insects, crustaceans, and mushrooms.
    Type: Grant
    Filed: June 2, 2014
    Date of Patent: June 11, 2019
    Assignees: ACTEIIVE CORPORATION
    Inventors: Kengo Sakaguchi, Yoshihiro Kanai, Tatsushi Ruike
  • Patent number: 10301653
    Abstract: Microorganisms that co-consume glucose with non-glucose carbohydrates, such as xylose, and methods of using same. The microorganisms comprise modifications that reduce or ablate the activity of a phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS) protein or modifications that reduce or ablate the activity of a phosphoglucose isomerase and a GntR. The PTS protein may be selected from an enzyme I (EI), an HPr, an FPr, and an enzyme IIGlc (EIIGlc). Additional modifications include reduction or ablation of the activity of a pyruvate formate lyase, a lactate dehydrogenase, and a fumarate reductase and inclusion of recombinant pyruvate decarboxylase and alcohol dehydrogenase genes. The microorganisms are particularly suited to co-consuming glucose and xylose in media containing these substrates and producing ethanol therefrom.
    Type: Grant
    Filed: July 6, 2015
    Date of Patent: May 28, 2019
    Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventors: Jennifer L. Reed, Joonhoon Kim
  • Patent number: 10260081
    Abstract: The present disclosure provides methods for generating sugars from a cellulosic biomass. The methods combine treatment of the biomass using a high-shear milling device and saccharification of the biomass to partially hydrolyze the biomass. The biomass can be saccharified either after or simultaneously with the high-shear milling treatment. The partially hydrolyzed biomass is then separated into a solids stream with saccharification enzymes, and a liquid stream with sugars. The solids stream and associated enzymes are further incubated under saccharification conditions to produce additional sugars, or are recycled and added to fresh biomass, which is saccharified under high-shear milling conditions. The methods result in improved conversion of cellulosic biomass to glucose.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: April 16, 2019
    Assignee: Edeniq, Inc.
    Inventors: Deepak Singh, Sandra Jacobson, Kris Ramos, Prachand Shrestha
  • Patent number: 10174351
    Abstract: The invention relates to a process for the hydrolysis of cellulose containing biomass which comprises a liquefaction step in which a first enzyme or first enzyme composition is added to liquefy at least part of the solids present in the biomass and to keep the viscosity of the cellulose containing biomass below 1000 cP, preferably below 800 cP, more preferably below 600 cP in the liquefaction step; followed by a saccharification step in which a second enzyme composition is added to form oligomeric and/or monomeric sugars; and whereby the first enzyme or first enzyme composition is different from the second enzyme composition; whereby the first enzyme or first enzyme composition comprises an endoglucanase; whereby the second enzyme composition comprises a cellulase; and whereby the first enzyme or first enzyme composition comprises more endogluconase than the second enzyme composition (expressed in protein wt %).
    Type: Grant
    Filed: January 9, 2014
    Date of Patent: January 8, 2019
    Assignee: DSM IP ASSETS B.V.
    Inventors: Johannes Petrus Smits, Michael Petrus Jozef Berkhout, Bertus Noordam
  • Patent number: 10106824
    Abstract: Materials and methods are described to produce xylitol from a mixture of hemicellulosic sugars by several routes. Examples include either as a direct co-product of a biorefinery or ethanol facility, or as a stand-alone product produced from an agricultural or forestry biomass feedstock including using, e.g. ethanol waste streams.
    Type: Grant
    Filed: January 14, 2011
    Date of Patent: October 23, 2018
    Assignees: The Board of Trustees of the University of Illinois, ZuChem, Inc.
    Inventors: Huimin Zhao, Nikhil Unni Nair, Michael Racine, Ryan Woodyer
  • Patent number: 10098916
    Abstract: This invention provides novel strains of the genus Lactobacillus which can be used to produce pharmaceutical products with probiotic properties for human or veterinary use. The strains of this invention possess metabolic features that are particularly suitable for treatment of disorders related to lactose malabsorption and intolerance, fructose malabsorption and intolerance, and bloating.
    Type: Grant
    Filed: December 19, 2014
    Date of Patent: October 16, 2018
    Assignee: MEDIS, D.O.O.
    Inventors: Helena Seme, Stefan Fujs, Kresimir Gjuracic, Hrvoje Petkovic, Gregor Kosec, Mateja Stempelj, Karmen Svigelj, Tone Strnad
  • Patent number: 10072099
    Abstract: The present invention is directed at a process for fractionating lignocellulosic biomass for the purpose of reducing processing costs, increasing delignification, reducing side-reactions, in particular reducing hemicelluloses degradation, improving cellulose hydrolysis and increasing the nativity of the obtained lignin, by performing organosolv fractionation of the biomass with a treatment liquid at a temperature below 170° C., wherein the treatment liquid comprises a non-hydroxylic organic solvent, in particular a ketone, water and some acid, and optionally subjecting the cellulose-enriched product stream to enzymatic hydrolysis.
    Type: Grant
    Filed: July 15, 2014
    Date of Patent: September 11, 2018
    Assignee: Stichting Energieonderzoek Centrum Nederland
    Inventors: Adrianus Theodorus Smit, Wouter Johannes Joseph Huijgen, Rudie Johan Hendrik Grisel
  • Patent number: 9982283
    Abstract: Provided is a biomass hydrothermal decomposition system which includes: a biomass supply unit that supplies a biomass raw material; hydrothermally decomposing the biomass raw material using pressurized hot water; a hydrothermal decomposition unit that dissolves lignin components and hemicellulose components in the pressurized hot water; a biomass solid fraction discharge unit that discharges a biomass solid fraction from the hydrothermal decomposition unit; an enzymatic liquefaction tank that is in connection with the biomass solid fraction discharge unit, and in which the discharged biomass solid fraction is introduced, and an enzyme is supplied to the biomass solid fraction to liquefy the biomass solid fraction; and a discharge unit that discharges the liquefied biomass solid fraction.
    Type: Grant
    Filed: July 11, 2012
    Date of Patent: May 29, 2018
    Assignee: MITSUBISHI HITACHI POWER SYSTEMS ENVIRONMENTAL SOLUTIONS, LTD.
    Inventors: Minoru Genta, Seiichi Terakura, Ryosuke Uehara, Seiji Kobayashi
  • Patent number: 9920389
    Abstract: Methods and systems are disclosed for the hydrolysis of mixed biomass. The methods include forming a mixture of at least two modified biomass feedstocks to achieve various benefits, such as maximizing sugar yields and minimizing the formation of degradation products.
    Type: Grant
    Filed: October 26, 2015
    Date of Patent: March 20, 2018
    Assignee: Renmatix, Inc.
    Inventors: Manuk Colakyan, Rory Hernan Jara-Moreno
  • Patent number: 9862980
    Abstract: The present invention provides a process for enzyme mediated hydrolysis of biomass for production of soluble sugars, wherein the said process comprises of steady addition of small portions of biomass to enzyme solution, enabling rapid solubilization of biomass. The process used for enzymatic saccharification allows for increased biomass loading, enzyme recycle and mitigation of substrate and product inhibitory effect. The recycling of unhydrolysed biomass along with soluble enzyme ensures complete reuse of the said enzyme for effective repeated hydrolysis thereby increasing the overall productivity of enzyme used.
    Type: Grant
    Filed: January 16, 2015
    Date of Patent: January 9, 2018
    Assignees: Department of Biotechnology, Institute of Chemical Technology
    Inventors: Arvind Mallinath Lali, Annamma Anil Odaneth, Sachinkumar Hiraman Birhade, Juliet Joanna Victoria, Sneha Chandrakant Sawant
  • Patent number: 9856498
    Abstract: The invention relates to processes of producing a fermentation product, comprising liquefying a starch containing material with an alpha-amylase; pre-saccharifying and/or saccharifying and fermenting using a fermentation organism in the presence of a carbohydrate source generating enzyme and a cellulolytic composition The invention also relates to methods of dewatering whole stillage.
    Type: Grant
    Filed: March 28, 2013
    Date of Patent: January 2, 2018
    Assignee: NOVOZYMES A/S
    Inventors: Thomas Rasmussen, Jeremy Saunders, James Croonenberghs, Zhengfang Kang, Joyce Craig, Michael John Akerman
  • Patent number: 9856605
    Abstract: In some variations, a process is provided for producing a pulp product at a biorefinery site, comprising: converting a woody cellulosic material to a first pulp stream; converting a non-woody cellulosic material to a second pulp stream; blending the second pulp stream into the first pulp stream; and recovering or further processing the blended pulp stream as a pulp product. Biorefinery site infrastructure may be shared between the woody and non-woody lines. Also, the process may include process integration of mass and/or energy between the woody and non-woody lines. The process may be a retrofit addition to a pulp plant, or a greenfield biorefinery site. The non-woody line also can generate fermentable sugars, for fermentation to ethanol (or other products). Through allocation of carbon credits from the ethanol to the pulp, the final pulp product life-cycle profile can be improved.
    Type: Grant
    Filed: March 30, 2015
    Date of Patent: January 2, 2018
    Assignee: API Intellectual Property Holdings, LLC
    Inventor: Theodora Retsina
  • Patent number: 9850511
    Abstract: A hydrothermal decomposition apparatus 17 as a biomass processing apparatus that decomposes a biomass material 11 into cellulose, hemicellulose, and lignin under a high temperature and high pressure condition to remove a lignin component and a hemicellulose component, a biomass solid discharging unit 18 that discharges a biomass solid (a hot-water insoluble component) 20 processed in the hydrothermal decomposition apparatus 17, and a slurrying vessel 21 communicating with the biomass solid discharging unit 18, into which water 19 is injected and the discharged biomass solid 20 is added to make it slurried are provided to an apparatus body 13, which is a processing vessel having a gas-liquid interface 13a.
    Type: Grant
    Filed: July 9, 2010
    Date of Patent: December 26, 2017
    Assignee: MITSUBISHI HITACHI POWER SYSTEMS ENVIRONMENTAL SOLUTIONS, LTD.
    Inventors: Hideo Suzuki, Yoshio Kuromi, Yoshitaka Kimura
  • Patent number: 9816113
    Abstract: Disclosed is a method for producing ethanol, including: culturing yeast transformed so as to display an enzyme on the cell surface in a medium containing particles of lignocellulosic biomass, thereby producing ethanol, wherein the enzyme is an enzyme involved in hydrolysis of the lignocellulosic biomass. The present invention makes it possible to provide a method for producing ethanol by which a high ethanol yield can be achieved from lignocellulosic biomass with lower initial cell concentration and added enzyme amount.
    Type: Grant
    Filed: September 3, 2014
    Date of Patent: November 14, 2017
    Assignees: Kansai Chemical Engineering Co., Ltd., Bio-Energy Corporation
    Inventors: Hideo Noda, Shinji Hama, Kohsuke Nakano
  • Patent number: 9809866
    Abstract: This specification describes a process of producing a monomeric sugar stream ligno-cellulosic biomass without enzymes or acid catalysts. This is accomplished by removing the water soluble C5 sugars from the ligno-cellulosic biomass feedstream, lowering the pH of the C5 solution with little or no addition of an acid, thermally treating the remaining ligno-cellulosic biomass, combining the thermally treated ligno-cellulosic biomass with the low pH C5 solution and then exposing the mixture to an elevated temperature greater than 80° C. for a time sufficient to hydrolyze at least some of the components of the ligno-cellulosic biomass. Preferably, the thermally treated ligno-cellulosic biomass is subjected to a fiber shives reduction step to reduce the amount of long fiber shives.
    Type: Grant
    Filed: October 31, 2014
    Date of Patent: November 7, 2017
    Assignee: Beta Renewable,m S.p.A.
    Inventors: Piero Ottonello, Paolo Torre, Beatriz Rivas Torres, Stefano Paravisi, Chiara Prefumo, Pietro Pastorino
  • Patent number: 9790529
    Abstract: Enzyme compositions with enhanced enzyme activity and/or thermophilic and psychrophilic stability are described. Additionally, methods and kits for making and using the enzyme compositions are provided.
    Type: Grant
    Filed: August 8, 2014
    Date of Patent: October 17, 2017
    Assignee: UNIVERSITY OF CALCUTTA
    Inventors: Krishanu Chakraborty, Arka Mukhopadhyay, Nalok Dutta, Anjan Kr. Dasgupta
  • Patent number: 9738943
    Abstract: The present invention describes a process for a controlled conversion of a biomass feedstock, wherein the process comprises the steps of: loading the biomass feedstock to at least one reactor; liquefaction of the biomass feedstock into a monomer and/or oligomer sugar mixture in said reactor by treatment in hot compressed liquid water (HCW) at sub- and/or super-critical condition; and removal of the monomer and/or oligomer sugar mixture, being the product molecules, to avoid continued detrimental decomposition.
    Type: Grant
    Filed: October 28, 2011
    Date of Patent: August 22, 2017
    Assignee: RENMATIX, INC.
    Inventors: Andreas Gram, Haukur Johannesson
  • Patent number: 9670509
    Abstract: The present disclosure relates to processes for production of an alcohol product from granular starch including a pre-treatment at an elevated temperature below the initial gelatinization temperature of the granular starch followed by simultaneous saccharification and fermentation, and optionally recovery of ethanol.
    Type: Grant
    Filed: February 10, 2014
    Date of Patent: June 6, 2017
    Assignee: NOVOZYMES A/S
    Inventors: Hans Sejr Olsen, Sven Pedersen, Rikke Monica Festersen
  • Patent number: 9663802
    Abstract: Processes are disclosed for economically and effectively removing co-produced oxygenated organic compound from an anaerobic, aqueous fermentation broth used for the bioconversion of syngas to product oxygenated organic compound. Nitrate anion is added to the broth and the broth is contacted with denitrifying microorganisms that bioconvert the nitrate and organic compounds in the broth to reduced nitrogen compound and carbon dioxide.
    Type: Grant
    Filed: July 9, 2014
    Date of Patent: May 30, 2017
    Assignee: SYNATA BIO, INC.
    Inventor: Robert Hickey
  • Patent number: 9657318
    Abstract: Provided is a method for preparing a lignocellulosic biomass hydrolysate having toxicity reduced or removed and being used for fermentation by microorganisms, comprising the steps of: pretreating, hydrolyzing, and using an electrochemical detoxification method.
    Type: Grant
    Filed: October 2, 2015
    Date of Patent: May 23, 2017
    Assignee: Korea Institute of Science and Technology
    Inventors: Youngsoon Um, Yunje Kim, Dongjin Suh, Kyung Min Lee, Jeong Myeong Ha
  • Patent number: 9637765
    Abstract: A process for pretreatment of a biomass material, comprising subjecting a biomass material to a biphasic mixture of water and supercritical CO2 at a temperature in the range of 150° C. to 250° C. under high pressure for a time of from 10 seconds to 100 minutes. In particular embodiments, the process is performed as a two-stage temperature process wherein an initial short high-temperature stage is conducted at a temperature of at least 200° C. for up to 20 minutes and a subsequent longer low-temperature stage is conducted at a temperature of at least 140° C. and up to 190° C. for 10-120 minutes.
    Type: Grant
    Filed: March 10, 2011
    Date of Patent: May 2, 2017
    Assignee: CORNELL UNIVERSITY
    Inventors: Jeremy Luterbacher, Larry P. Walker, Jefferson W. Tester
  • Patent number: 9631057
    Abstract: In one embodiment the instant invention generally pertains to a method for producing glucose for fermentation. The method comprises first treating a biomass with acid and heat under conditions sufficient to produce a composition mixture comprising cellulose suitable for enzymatic hydrolysis. Next, at least a portion of the cellulose of step (a) is enzymatically hydrolyzed under conditions sufficient to form a composition comprising glucose. The glucose is then fermented. Advantageously, one or more reaction conditions are more efficient because they are selected by first measuring an initial hydrolysis rate of said biomass and then selecting one or more appropriate reaction conditions based upon said initial hydrolysis rate.
    Type: Grant
    Filed: November 9, 2010
    Date of Patent: April 25, 2017
    Assignees: Texaco Inc., Georgia Tech Research Corporation
    Inventors: Matthew James Realff, Ronald W. Smith, Prabuddha Bansal, Andreas Sebastian Bommarius, Melanie Hall, Jay Hyung Lee
  • Patent number: 9587254
    Abstract: The present invention provides a method for producing a useful substance efficiently from cellulose without using any cellulase preparation. According to the disclosures of the present specification, in the production of a useful substance from a cellulose-containing material, the cellulose-containing material is brought into contact with an ionic liquid to cause the ionic liquid to permeate the cellulose-containing material, and a carbon source comprising the cellulose in the cellulose-containing material is simultaneously saccharified and fermented by a cellulase-producing microorganism in the presence of the ionic liquid.
    Type: Grant
    Filed: August 5, 2010
    Date of Patent: March 7, 2017
    Assignees: KABUSHIKI KAISHA TOYOTA CHUO KENKYUSHO, TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Sung-Jin Jo, Risa Nakamura, Satoshi Katahira, Nobuhiro Ishida, Haruo Takahashi, Naoko Takahashi, Kazuhide Tabata, Kazunori Nakashima, Chiaki Ogino, Akihiko Kondo
  • Patent number: 9572364
    Abstract: The present invention includes a method for enhancing the taste of a food product, which includes the steps of culturing a submerged mycelial liquid tissue culture in a media, collecting the supernatant fluid of the submerged mycelial liquid tissue culture, and adding the collected supernatant fluid to a food product in an amount sufficient to enhance the food product's taste. In one embodiment, the mycelial liquid tissue culture comprises C. sinensis, and the culture step is carried out for between about one and sixty days. The food products include quinoa, amaranth, whole wheat, stevia plant parts, steviol glycoside, aspartame, acesulfame-K, sucralose, carbohydrates, salt substitutes such as potassium chloride, monk fruit, cacao, cacao liquor, tea, ginseng, pea protein, sugar alcohol, coffee, cranberry, grapefruit, pomegranate, coconut, wine, beer, liquor and spirits. The present invention also includes products made by the disclosed methods.
    Type: Grant
    Filed: May 2, 2016
    Date of Patent: February 21, 2017
    Assignee: MYCOTECHNOLOGY, INC.
    Inventors: James Patrick Langan, Brooks John Kelly, Huntington Davis, Bhupendra Kumar Soni
  • Patent number: 9540664
    Abstract: Systems and methods for improving ethanol yield are provided. A feedstock is ground suitably fine for use in fermentation. The feedstock may include corn or any other suitable material. In some cases, the feedstock undergoes a fractionation prior to grinding. The ground feedstock may be slurried with water and enzymes to facilitate conversion of the starch in the feedstock to sugars. The slurry may be about 35% solids. After being slurried, an ethanologen may be added. Additionally, the pH of the slurry may be adjusted to between 4.2 and 5.2 to facilitate the priming A primer is added to the slurry. The primer may include any weak acid, and in some embodiments includes acetic acid. Acetic acid, when used as a primer, may be added at a concentration of between 1200 and 3600 parts per million. The slurry is fermented to produce improved yields of ethanol.
    Type: Grant
    Filed: March 14, 2012
    Date of Patent: January 10, 2017
    Assignee: POET Research, Inc
    Inventors: Stephen M. Lewis, Neelakantam V. Narendranath
  • Patent number: 9534242
    Abstract: Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can convert feedstock materials to a sugar solution, which can then be fermented to produce ethanol. Biomass feedstock is saccharified in a vessel by operation of a jet mixer, the vessel also containing a fluid medium and a saccharifying agent.
    Type: Grant
    Filed: June 19, 2015
    Date of Patent: January 3, 2017
    Assignee: XYLECO, INC.
    Inventors: Marshall Medoff, Thomas Craig Masterman
  • Patent number: 9518274
    Abstract: A process for producing bioethanol includes the steps of pretreatment (consisting in destructuring the lignocellulosic vegetable raw material by placing it in the presence of a mixture containing formic acid, acetic acid and water, then in separating cellulose), of enzymatic hydrolysis and of alcoholic fermentation, characterized in that it includes, prior to the enzymatic hydrolysis, a step of partial elimination of the lignins so as to obtain a residual overall level of lignins (T), expressed as percentage by weight, which is non-zero and which is included in a range determined by a lower limit, and an upper limit Bsup, respectively equal to 0.30% and 4%. In order to obtain conditions of acidification before the enzymatic hydrolysis step, the process includes a step for re-acidification of the mixture, which is carried out with an acid, or of a mixture of acids, of determined pKa, and preferably with weak organic.
    Type: Grant
    Filed: October 5, 2011
    Date of Patent: December 13, 2016
    Assignee: COMPAGNIE INDUSTRIELLE DE LA MATIERE VEGETALE—CIMV
    Inventors: Michel Delmas, Bouchra Benjelloun Mlayah
  • Patent number: 9487840
    Abstract: Conventionally, sugarcane processing avoids leaving residual sucrose in the bagasse, since the bagasse will be burned and the value of the sucrose would be lost. However, when coupled with a Green Power+® process to extract hemicelluloses, sucrose may also be extracted and recovered from the bagasse. In some variations, a process includes mechanically treating a feedstock to generate a sucrose-rich stream and lignocellulosic material that intentionally retains a significant amount of the initial sucrose in the feedstock; extracting the lignocellulosic material with steam and/or hot water to produce cellulose-rich solids and an extract liquor containing hemicellulosic oligomers and sucrose; and then hydrolyzing the hemicellulosic oligomers into a hemicellulose sugar stream. Each of the sucrose-rich stream and the hemicellulose sugar stream (containing the starting residual sucrose) may be recovered or further processed (e.g., fermented to ethanol).
    Type: Grant
    Filed: September 15, 2014
    Date of Patent: November 8, 2016
    Assignee: API Intellectual Property Holdings, LLC
    Inventors: Theodora Retsina, Ryan O'Connor
  • Patent number: 9458569
    Abstract: The present disclosure comprises methods, apparatus, components, and techniques for pretreatment of biomaterials using targeted wet oxidation. The targeted wet oxidation pretreatment is an upstream method for converting solid biomass into fuels and/or specialty chemicals. Embodiments of the present disclosure comprise methods carried out on biomaterials to selectively oxidize lignin components of the biomass, thereby resulting in bio accessible/digestible biomass fibers. In embodiments of the present disclosure, such methods may comprise pretreatment processes to prepare the biomaterials for a subsequent fermentation or other like conversion may be carried out to result in useful bio fuels or other bioproducts.
    Type: Grant
    Filed: January 16, 2014
    Date of Patent: October 4, 2016
    Assignee: CLEAN-VANTAGE LLC
    Inventors: Richard Alan Merrill, Kenn Petersen
  • Patent number: 9434961
    Abstract: A continuous process for the recovery of ethanol from hemicellulose and cellulose from lignocellulosic biomass. Yield of fermentable sugars can be maximized by continuous operation of the pre-treatment system and careful selection of pretreatment conditions including the addition of only small amounts of dilute mineral acid and low pressure. With this approach, the xylose component that is mainly present in its unfermentable oligomeric form in known pre-hydrolysis Kraft processes can be recovered more efficiently and as a monomer that can be fermented by xylose fermenting yeasts and bacteria. Due to the use of only dilute acids, there is a very low loss of glucose and xylose hence very low production of toxic chemicals (e.g. HMF, furfural) in the pretreatment step. The resulting overall fermentation efficiency of both hexose and pentose sugars is 90% of the theoretical maximum.
    Type: Grant
    Filed: November 8, 2011
    Date of Patent: September 6, 2016
    Assignee: GREENFIELD SPECIALTY ALCOHOLS INC.
    Inventors: Frank A. Dottori, Robert Ashley Cooper Benson, Régis-Olivier Benech, Richard Romeo Lehoux, Christopher Bruce Bradt
  • Patent number: 9428772
    Abstract: A method for producing fermentation products from lignocellulosic biomass is provided. Lignocellulosic biomass is composed of lignocellulosic fibers which are hollow and primarily contain cellulose, hemicellulose and lignin. Lignin is concentrated in the outer fiber wall and glues the fibers into bundles, but the inner fiber wall has a much lower concentration of lignin and has more easily accessible cellulose and hemicellulose. This method uses vacuum infusion to infuse enzymes into the lumen (hollow center) of lignocellulosic fibers to hydrolyze the hemicellulose and cellulose to produce sugars and oligomers, and then uses cycles of vacuum pressure to pump these homogeneous reagents and sugars and oligomers into and out of the lumen. These reagents are homogenized by mixing the reagents with process water using turbulent mixing to produce a homogeneous reagent. The sugars may be fermented, such as with yeast, to a fermentation product, such as ethanol or butanol.
    Type: Grant
    Filed: October 27, 2015
    Date of Patent: August 30, 2016
    Inventor: Edward Brian Hamrick
  • Patent number: 9416374
    Abstract: A method for the treatment of lignocellulose-containing materials wherein such lignocellulose-containing materials, normally resistant to biotreatment, are first subjected to pyrolysis to produce pyrolysis products including pyrogas and char. Exemplary applications of the method include pyrolytic pre-treatment of wastewater sludges, cellulosic wastes, wood, peat, plant residues, low-grade coal, and the like. The pyrogas is introduced into digester sludge in an anaerobic digester.
    Type: Grant
    Filed: February 25, 2015
    Date of Patent: August 16, 2016
    Assignee: ANAERGIA INC.
    Inventor: F. Michael Lewis
  • Patent number: 9410216
    Abstract: A sugar mixture comprising: monosaccharides; oligosaccharides in a ratio ?0.06 to total saccharides; disaccharides in a ratio to total saccharides ?0.05; pentose in a ratio to total saccharides ?0.05; at least one alpha-bonded di-glucose; and at least one beta-bonded di-glucose. Also disclosed are methods to make and/or use such mixtures.
    Type: Grant
    Filed: June 26, 2011
    Date of Patent: August 9, 2016
    Assignee: Virdia, Inc.
    Inventors: Aharon Meir Eyal, Robert P. Jansen, Revital Mali, Asher Vitner
  • Patent number: 9399782
    Abstract: The invention relates to methods for producing a fermentation product from a lignocellulose-containing material comprising i) pre-treating lignocellulose-containing material; ii) hydrolyzing pre-treated lignocellulose-containing material; iii) fermenting using a fermenting organism; wherein fermentation is initiated and carried out at: a) a fermentation organism cell count in the range from 10-250×1010 cells per L fermentation medium; or b) a fermentation organism concentration in the range from 2-90 g dry weight fermenting organism per L fermentation medium.
    Type: Grant
    Filed: June 27, 2008
    Date of Patent: July 26, 2016
    Assignee: NOVOZYMES A/S
    Inventors: Mads Torry Smith, Guillermo Coward-Kelly, Dan Nilsson, Zhengfang Kang, Prashant Iyer, Randy Deinhammer
  • Patent number: 9388442
    Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) or other materials are processed to produce useful intermediates and products, such as energy, fuels, foods or materials. For example, systems and methods are described that can be used to treat feedstock materials, such as cellulosic and/or lignocellulosic materials, in a vault in which the walls and optionally the ceiling include discrete units. Such vaults are re-configurable.
    Type: Grant
    Filed: June 9, 2014
    Date of Patent: July 12, 2016
    Assignee: Xyleco, Inc.
    Inventors: Marshall Medoff, Thomas Craig Masterman, Robert Paradis
  • Patent number: 9371550
    Abstract: Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, novel systems, methods and equipment for conveying and/or cooling treated biomass are described.
    Type: Grant
    Filed: June 9, 2014
    Date of Patent: June 21, 2016
    Assignee: XYLECO, INC.
    Inventors: Marshall Medoff, Thomas Craig Masterman, Robert Paradis
  • Patent number: 9359619
    Abstract: Described are processes for the liquefaction of lignocellulosic biomass under the digestive action of dicarboxylic acid(s). Such digests can exhibit enhanced flowability, reduced volume, and significant biomass conversion to dissolved components, and can in some embodiments be further liquefied by contact with an enzyme. Products resultant of these steps can be used for their sugar content to manufacture biofuels or other products.
    Type: Grant
    Filed: January 30, 2013
    Date of Patent: June 7, 2016
    Assignee: Purdue Research Foundation
    Inventors: Michael R. Ladisch, Nathan Mosier, Youngmi Kim