Carrier Is Carbohydrate Patents (Class 435/178)
  • Patent number: 11912978
    Abstract: The present disclosure relates to systems and methods for flow-through separation of paramagnetic particle-bound cells in a cell suspension containing both bound and unbound cells as well as systems and methods for removing paramagnetic particles from paramagnetic particle-bound cells or from a cell suspension with unbound cells. It further relates to a flow-through magnetic separation/debeading module and a flow-through spinning membrane debeading module.
    Type: Grant
    Filed: October 15, 2021
    Date of Patent: February 27, 2024
    Assignees: Novartis AG
    Inventors: Fabio Fachin, Rodney Rietze, Michael R. Greene, Lan Cao
  • Patent number: 11607345
    Abstract: Methods of entrapping enzymes in chitosan matrices using mild conditions, such as methods that are compatible with the entrapment of acid-sensitive and/or heat sensitive enzymes, are described. The methods can provide biocatalytic materials with high chitosan to enzyme mass ratios where one or more enzymes can remain active and stably entrapped in the chitosan matrix for many months and/or after repeated washings. Also described are materials including a solid chitosan matrix having at least one enzyme stably entrapped therein, including fabrics, textiles and other flexible materials at least partially coated with one or more layer of the solid chitosan matrix having at least one enzyme stably entrapped therein and the use of the materials as biocatalyst systems, for example in biogas upgrading and other applications.
    Type: Grant
    Filed: February 28, 2020
    Date of Patent: March 21, 2023
    Assignee: North Carolina State University
    Inventors: Sonja Salmon, Yue Yuan
  • Patent number: 11491721
    Abstract: Disclosed herein is a multicellular lay-up process. The process comprises the steps of: a) forming a core material, b) forming a capsule material, c) encapsulating the core with the capsule material, d) adding the capsule to a substrate, and e) exposing the capsule to at least one bioactivating agent.
    Type: Grant
    Filed: June 8, 2017
    Date of Patent: November 8, 2022
    Assignee: Attenborough Dental Laboratories Limited
    Inventors: Edward John Attenborough, Robert Stevens
  • Patent number: 11236187
    Abstract: The disclosure provides an amphiphilic porous cyclodextrin polymer: cyclodextrin is grafted with aromatic molecules, and then alkylated with biphenyl benzylidene chloride and p-xylylene dichloride through Friedel-Crafts reaction, to obtain a porous polymer with an ultrahigh specific surface area. This material has very high adsorption capacity and can be reused for simple regeneration. Because the hydroxy groups of cyclodextrin are partially retained, the hydrophilicity of the material surface is improved, allowing the adsorption rate to become faster. Moreover, there are a variety of adsorption mechanisms, and hydrophobic and hydrophilic pollutants can be adsorbed simultaneously.
    Type: Grant
    Filed: July 27, 2020
    Date of Patent: February 1, 2022
    Inventors: Xianchuan Xie, Yizhou Tu
  • Patent number: 11213609
    Abstract: A spherical particle comprising decellularized omentum being between 1 nM-300 ?M in diameter is disclosed. In some embodiments, the particle comprises biological cells. In other embodiments, the particle comprises a biomolecule. Uses of the particles are also disclosed.
    Type: Grant
    Filed: December 15, 2016
    Date of Patent: January 4, 2022
    Assignee: Ramot at Tel-Aviv University Ltd.
    Inventors: Tal Dvir, Assaf Shapira, Michal Shevach, Idan Gal
  • Patent number: 11191789
    Abstract: Cells derived from postpartum tissue and methods for their isolation and induction to differentiate to cells of a chondrogenic or osteogenic phenotype are provided by the invention. The invention further provides cultures and compositions of the postpartum-derived cells and products related thereto. The postpartum-derived cells of the invention and products related thereto have a plethora of uses, including but not limited to research, diagnostic, and therapeutic applications, for example, in the treatment of bone and cartilage conditions.
    Type: Grant
    Filed: June 25, 2004
    Date of Patent: December 7, 2021
    Assignee: Depuy Synthes Products, Inc.
    Inventors: Anthony J. Kihm, Agnieszka Seyda, Sridevi Dhanaraj, Ziwei Wang, Alexander M. Harmon, Ian Ross Harris, Darin J. Messina, Sanjay Mistry, Anna Gosiewska, Chin-Feng Yi
  • Patent number: 11124820
    Abstract: Provided is a sample processing method that liquefies a medium solution by making a liquid that liquefies the medium solution act on a sample formed by gelating or solidifying the medium solution that is supported by a substrate while an observation subject is included therein, while maintaining a state in which the medium solution is supported by the substrate while the observation subject is included therein.
    Type: Grant
    Filed: December 17, 2018
    Date of Patent: September 21, 2021
    Inventor: Katsunori Ogo
  • Patent number: 10316312
    Abstract: The present invention generally relates to compositions and methods of delivering substances in a dry mode, wherein the compositions include an inert carrier substrate having a porous structure, microorganisms loaded throughout the pores of the inert carrier substrate, and a surface layer disposed on the outer surface of the inert carrier substrate, wherein the surface layer is permeable to molecules that aid in cell growth of the microorganisms such that the composition is operable to allow for increased propagation of the microorganisms within the pores of the inert carrier substrate as compared to another composition having an absence of the surface layer.
    Type: Grant
    Filed: October 5, 2011
    Date of Patent: June 11, 2019
    Assignee: DRYLET, LLC
    Inventors: Ramiro Trevino, Steven R. Ellis
  • Patent number: 10190115
    Abstract: Provided herein are methods, compositions, and kits for assays, many of which involve amplification reactions such as digital PCR or droplet digital PCR. The assays may be used for such applications as sequencing, copy number variation analysis, and others. In some cases, the assays involve subdividing a sample into multiple partitions (e.g., droplets) and merging the partitions with other partitions that comprise adaptors with barcodes.
    Type: Grant
    Filed: September 22, 2014
    Date of Patent: January 29, 2019
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Serge Saxonov
  • Patent number: 10080997
    Abstract: A method and apparatus for generating droplets with a high level of uniformity in liquid systems that present a low interfacial tension. This method and apparatus utilize the breakup of the dispersed phase in a controlled fashion by periodically varying the pressure that drives the fluids so as to successfully generate emulsions with a good control over the size. The method and apparatus can be used for the formation of simple emulsion or double emulsion where a larger droplet contains one or more smaller droplets.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: September 25, 2018
    Inventors: Ho Cheung Shum, Alban Sauret, Zida Li, Yang Song
  • Patent number: 10076600
    Abstract: This invention uses “Targeted Apheresis” to treat patients with Rheumatoid Arthritis and other autoimmune and inflammatory disorders. “Targeted Apheresis” is a process whereby only the pathogenic and pro-inflammatory elements associated with the disease symptoms are simultaneously and selectively removed from the blood by passing the blood through an extracorporeal affinity device containing selective binding agents. Removal of these pathogenic and pro-inflammatory elements will ameliorate the symptoms of autoimmune disease and may prolong the period of disease remission.
    Type: Grant
    Filed: December 22, 2015
    Date of Patent: September 18, 2018
    Inventors: Henry J. Smith, James R. Smith
  • Patent number: 9944920
    Abstract: The present application discloses immobilized enzymes and immobilized enzyme materials comprising a crosslinked enzyme having a support material which includes a biomass material different than the biomass used to initially derive the enzyme. Optionally, the immobilized enzyme further includes a polymeric material and/or the biomass which was used to initially derive the enzyme. The resulting immobilized enzyme materials may be biodegradable. The present application also discloses methods of making and using the disclosed immobilized enzyme materials.
    Type: Grant
    Filed: November 13, 2012
    Date of Patent: April 17, 2018
    Assignee: Guild Associates, Inc.
    Inventors: Augustine A. DiNovo, Dominic P. DiNovo, David A. Schofield, Matthew F. Smiechowski, Francis H. Verhoff
  • Patent number: 9724367
    Abstract: The present invention concerns phase changing injectable formulations for organ augmentation containing active agents, such as bioactive cell populations, and methods of making and using the same.
    Type: Grant
    Filed: November 10, 2011
    Date of Patent: August 8, 2017
    Assignee: RegenMed (Cayman) LTD.
    Inventors: Joydeep Basu, Richard Payne, Neil F. Robbins, Oluwatoyin A. Knight, Deepak Jain, Craig R. Halberstadt, Monica A. Serban
  • Patent number: 9428768
    Abstract: The present invention relates to improved methods for producing adenovirus compositions wherein host cells are grown in a bioreactor and purified by size partitioning purification to provide purified adenovirus compositions.
    Type: Grant
    Filed: October 28, 2009
    Date of Patent: August 30, 2016
    Assignee: Crucell Holland B.V.
    Inventors: Hai Pham, Shuyuan Zhang, Peter Clarke
  • Patent number: 9388401
    Abstract: The present invention relates antidotes to anticoagulants targeting factor Xa. The antidoes are factor Xa protein derivatives that bind to the factor Xa inhibitors thereby substantially neutralizing them but do not assemble into the prothrombinase complex. The derivatives describe herein lack or have reduced intrinsic coagulant activity. Disclosed herein are methods of stopping or preventing bleeding in a patient that is currently undergoing anticoagulant therapy with a factor Xa inhibitor.
    Type: Grant
    Filed: July 21, 2014
    Date of Patent: July 12, 2016
    Assignee: Portola Pharmaceuticals, Inc.
    Inventors: Genmin Lu, David R. Phillips, Patrick Andre, Uma Sinha
  • Patent number: 9181536
    Abstract: Stabilized angiogenin compositions and methods of preparing a stabilized angiogenin compositions by covalent immobilization on a naturally occurring substrate, such as a polysaccharide substrate, are disclosed. In particular, the polysaccharide substrate includes galactose-rich polysaccharide.
    Type: Grant
    Filed: August 27, 2014
    Date of Patent: November 10, 2015
    Assignee: NAIDU LP
    Inventor: A. Satyanarayan Naidu
  • Patent number: 9175261
    Abstract: Cell-based compositions and methods of their use to inhibit an adverse immune response such as graft versus host disease or rejection of transplanted tissue in a transplant recipient that is histocompatibility mismatched to the transplant donor are disclosed. The compositions and methods utilize postpartum-derived cells, such as cells derived from the placenta or umbilicus.
    Type: Grant
    Filed: December 15, 2006
    Date of Patent: November 3, 2015
    Assignee: DePuy Synthes Products, Inc.
    Inventors: Alexander M. Harmon, Janet E. Davis
  • Patent number: 9028857
    Abstract: The present invention relates to a method for preparing a porous scaffold for tissue engineering. It is another object of the present invention to provide a porous scaffold obtainable by the method as above described, and its use for tissue engineering, cell culture and cell delivery. The method of the invention comprise the steps consisting of a) preparing an alkaline aqueous solution comprising an amount of at least one polysaccharide and one cross-linking agent b) freezing the aqueous solution of step a) c) sublimating the frozen solution of step b). characterized in that step b) is performed before the cross-linking of the polysaccharide occurs in the solution of step a).
    Type: Grant
    Filed: October 10, 2008
    Date of Patent: May 12, 2015
    Assignees: INSERM (Institut National de la Sante et de la Recherche Medicale), Universite Paris 7—Denis Diderot
    Inventors: Catherine Le Visage, Didier Letourneur, Frederic Chaubet, Aude Autissier
  • Publication number: 20150125924
    Abstract: A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
    Type: Application
    Filed: June 2, 2014
    Publication date: May 7, 2015
    Inventors: Aldis Darzins, Lance P. Encell, Dieter Klaubert, Georgyi V. Los, Mark McDougall, Keith V. Wood, Monika G. Wood, Chad Zimprich
  • Patent number: 8986955
    Abstract: The present invention provides a method and a system for increasing thermal stability of a target protein comprising fusing a starch binding protein (SBP) with the target protein to form a SBP-tagged recombinant protein and combining the SBP-tagged recombinant protein with a SBP-binding matrix. The present invention also provides a method for retaining an activity of a target protein in aquatic environment comprising fusing a starch binding protein (SBP) with the target protein to form a SBP-tagged recombinant protein and combining the SBP-tagged recombinant protein with a SBP-binding matrix.
    Type: Grant
    Filed: October 8, 2012
    Date of Patent: March 24, 2015
    Assignee: Simpson Biotech Co., Ltd.
    Inventors: Margaret Dah-Tsyr Chang, Chia-Chin Sheu
  • Publication number: 20150082468
    Abstract: The invention features an “inverse patterning” or “Intaglio-Void/Embed-Relief Topographic (In VERT) molding” manufacturing process for generating high-resolution three-dimensional (3D) multi-cellular microstructures in distinct cellular compartments of a single hydrogel. The platform has general utility in the development of engineered tissues for human therapies, drug testing, and disease models. Additionally, the platform can serve as a model system for studying 3D cell-cell interactions in fields as diverse as stem cell biology to the development of cancer therapeutics.
    Type: Application
    Filed: February 28, 2013
    Publication date: March 19, 2015
    Inventors: Sangeeta N. Bhatia, Kelly R. Stevens
  • Patent number: 8932858
    Abstract: Hydrophobically modified polysaccharide compositions, articles incorporating the compositions, and methods for cell culture, automatic release of cells, and release of cell culture coating from a substrate or the cell culture articles incorporating the coating compositions, as defined herein.
    Type: Grant
    Filed: March 4, 2009
    Date of Patent: January 13, 2015
    Assignee: Corning Incorporated
    Inventors: Wendy A. Baker, Dana C. Bookbinder, Theresa Chang, Robert R. Hancock, Jr.
  • Publication number: 20150010979
    Abstract: Stabilized angiogenin compositions and methods of preparing a stabilized angiogenin compositions by covalent immobilization on a naturally occurring substrate, such as a polysaccharide substrate, are disclosed. In particular, the polysaccharide substrate includes galactose-rich polysaccharide.
    Type: Application
    Filed: August 27, 2014
    Publication date: January 8, 2015
    Inventor: A. Satyanarayan Naidu
  • Patent number: 8911980
    Abstract: The present invention provides a method for producing a ground product of a lipase powder composition comprising an immobilized lipase in which a lipase derived from Thermomyces sp. is immobilized to a silica carrier and a hydrophilic powder other than a silica carrier, the method comprising the steps of wetting the hydrophilic powder with oil; separating the hydrophilic powder wetted with oil from the oil to obtain an oil-containing hydrophilic powder; mixing the oil-containing hydrophilic powder and the immobilized lipase at a ratio of 0.45:1 to 0.85:1 based on mass of the hydrophilic powder and the immobilized lipase before oil wetting; and grinding the obtained mixture, the steps being conducted in the above order.
    Type: Grant
    Filed: March 26, 2010
    Date of Patent: December 16, 2014
    Assignee: The Nisshin Oillio Group, Ltd.
    Inventors: Junko Suzuki, Yoshie Yamauchi, Yuko Toyama, Satoshi Negishi
  • Publication number: 20140335588
    Abstract: A process for production of an encapsulated cell product, the process comprises the steps of concentrating cells from a propagation medium using a tangential flow filtration system. Mixing the concentrated cells with an encapsulation medium to form a cell encapsulation mixture. Polymerizing, gelling, or cross-linking the cell encapsulation mixture to form an encapsulated cell product.
    Type: Application
    Filed: April 25, 2013
    Publication date: November 13, 2014
    Applicant: GEOSYNFUELS, LLC
    Inventors: Lisa Beckler Andersen, John H. Evans, Christine A. Singer
  • Patent number: 8877496
    Abstract: The invention provides a method for transferring cells to carriers, including: (a) providing a hydrophobic cell culture container or a cell culture container coated with a hydrophobic material on a bottom thereof; (b) adding carriers which are more hydrophilic than the hydrophobic cell culture container or hydrophobic materials and a culture medium containing cells into the hydrophobic cell culture container or the cell culture container coated with the hydrophobic material on the bottom thereof; and (c) culturing the cells, wherein the cells attach to the carriers and grow.
    Type: Grant
    Filed: June 5, 2009
    Date of Patent: November 4, 2014
    Assignee: National Taiwan University
    Inventors: Chin-Hsiung Hsieh, Yi-You Huang
  • Patent number: 8828698
    Abstract: Stabilized angiogenin compositions and methods of preparing a stabilized angiogenin compositions by covalent immobilization on a naturally occurring substrate, such as a polysaccharide substrate, are disclosed. In particular, the polysaccharide substrate includes galactose-rich polysaccharide.
    Type: Grant
    Filed: April 12, 2007
    Date of Patent: September 9, 2014
    Assignee: Naidu LP
    Inventor: A. Satyanarayan Naidu
  • Publication number: 20140242181
    Abstract: The present invention aims to generate a fibrin gel for cell proliferation and transport using the patient's own blood or compatible blood. The gel-preparation process consists in taking a quantity of blood from the patient, using sodium citrate as anticoagulant, separating off the citrated plasma by centrifugation, and resuspending the cells to be transported in the plasma. CaCl2 is added to the resulting suspension to form the gel (either as an isolated coagulate or within a porous chitosan, gelatin or hyaluronic acid matrix), and the gel is cultured in an incubator to promote cell growth. The invention will be of use in cell growth for the purposes of implantation in cutaneous, cartilaginous, gingival and bone lesions, inter alias, and in the field of tissue engineering.
    Type: Application
    Filed: August 3, 2012
    Publication date: August 28, 2014
    Inventors: Manuel Eduardo Young Anze, Carolina Ruth Weinstein Oppenheimer, Donald Irving Brown Gonzalez, Miguel Angel Fuentes Chandia, Ricardo Andres Ceriani Fernandez, Fernando Antonio Albornoz Marquez, Cristian Andres Acevedo Gutierrez
  • Publication number: 20140220085
    Abstract: In an embodiment of the disclosure, a biomedical material is provided. The biomedical material includes a biocompatible material having a surface and a carrier distributed over the surface of the biocompatible material, wherein both of the biocompatible material and the carrier have no charges, one of them has charges or both of them have charges with different electricity. The biomedical material is utilized for dentistry, orthopedics, wound healing or medical beauty and applied in the repair and regeneration of various soft and hard tissues.
    Type: Application
    Filed: April 11, 2014
    Publication date: August 7, 2014
    Applicant: Industrial Technology Research Institute
    Inventors: Pei-Yi TSAI, Yi-Hung WEN, Zhi-Jie HUANG, Pei-Shan LI, Hsin-Hsin SHEN, Yi-Hung LIN, Chih-Hung CHEN
  • Patent number: 8759056
    Abstract: A process for the production of granules or pellets containing filamentous fungi is described comprising the steps of selecting and growing filamentous fungi in a suitable culture medium for a predetermined amount of time, adding a gelling agent and at least one carrier to said culture medium, so as to obtain a mixture, subjecting said mixture to gelling through contact, drop by drop, with a solution containing a calcium salt thus obtaining gelled pellets or granules containing said filamentous fungi and drying said gelled pellets or granules to a moisture content of 13-18%.
    Type: Grant
    Filed: February 8, 2005
    Date of Patent: June 24, 2014
    Assignee: Urea Casale SA
    Inventor: Elisabeth Panchaud-Mirabel
  • Patent number: 8741637
    Abstract: The invention relates to the use of encapsulates of cancer cells, in agarose coated, agarose containing beads, for isolating chemotherapeutic resistant cells which have at least one stem cell property, such as expression of OCT4. The cells thus isolated are also a feature of the invention, as is a method for screening for potential therapeutic agents.
    Type: Grant
    Filed: November 22, 2011
    Date of Patent: June 3, 2014
    Assignee: The Rogosin Institute, Inc.
    Inventors: Lawrence Gazda, Barry Smith
  • Publication number: 20140134701
    Abstract: The present invention discloses a bacteriorhodopsin-containing bioactive gel and a method for fabricating the same, wherein a thickening polymeric compound is suspended in water to form a base gel having appropriate glutinosity and suitable for skin, and wherein a bacteriorhodopsin-containing culture fluid, which is obtained via culturing halobacteria in a spent culture fluid, is integrated with the glutinous base gel to form the bacteriorhodopsin-containing bioactive gel. The bioactive gel of the present invention is abundant of vitamins, minerals, amino acids, etc. and thus very useful in beauty treatment and dermatological treatment. The present invention is also expected to have high commercial potential in many other fields.
    Type: Application
    Filed: December 27, 2012
    Publication date: May 15, 2014
    Applicant: B.R.L. LABORATORY INC.
    Inventor: Alexander BRASLAVSKIY
  • Publication number: 20140127776
    Abstract: Compositions, articles, apparatus, methods and systems are provided for the growth of algae immobilized on a support in a gaseous environment supplying access to sources of carbon dioxide and light, and for subsequent harvesting and biomass processing.
    Type: Application
    Filed: June 13, 2012
    Publication date: May 8, 2014
    Inventor: Gaston Picard
  • Publication number: 20140127775
    Abstract: A process for converting biomass hydrolysate into biofuel, the process comprising the steps of: obtaining a biomass hydrolysate solution comprising monosaccharides; immobilizing Pachysolen tannophilus; contacting the solution with the immobilized Pachysolen tannophilus; and recovering a fermented biofuel.
    Type: Application
    Filed: May 17, 2013
    Publication date: May 8, 2014
    Applicant: GeoSynFuels, LLC
    Inventors: Lisa Beckler Andersen, John H. Evans, IV, Christine A. Singer
  • Publication number: 20140127290
    Abstract: Disclosed are devices and methods for encapsulating living cells, microencapsulated cells produced by the disclosed devices and methods, as well as methods of using the disclosed microencapsulated cells.
    Type: Application
    Filed: November 8, 2013
    Publication date: May 8, 2014
    Inventors: Xiaoming He, Pranay Agarwal, Shuting Zhao
  • Publication number: 20140127774
    Abstract: Uniform, functional polymer patches can be attached to a fraction of the surface area of living individual cells. These surface-modified cells remain viable after attachment of the functional patch. The patch does not completely occlude the cellular surface from the surrounding environment. Functional payloads carried by the patch may include, for example, drugs or other small molecules, peptides, proteins, thermally responsive polymers, and nanoparticles, or any other material that can be incorporated in a polymer patch of subcellular dimensions. The patch can include one or more polyelectrolyte multilayers (PEMs).
    Type: Application
    Filed: November 2, 2012
    Publication date: May 8, 2014
    Applicant: Massachusetts Institute of Technology
    Inventors: Albert J. Swiston, Michael F. Rubner, Robert E. Cohen, Darrell J. Irvine
  • Patent number: 8715983
    Abstract: The present invention is directed to a hydrogel network comprised of a physically cross-linked polymer and a chemically cross-linked polymer or physically entangled copolymer containing living cells, such as chondrocytes, encapsulated therein. In a preferred aspect, the physically cross-linked polymer is selected from the group consisting of thermally gelling polysaccharides and proteins, such as agarose or gelatin, and the chemically cross-linked or physically entangled polymer is synthesized from a water-soluble vinyl monomer, either as a homopolymer or copolymer, such as polyethylene glycol diacrylate (“PEG-DA”) and 2-hydroxyethyl methacrylate (“HEMA”).
    Type: Grant
    Filed: September 5, 2012
    Date of Patent: May 6, 2014
    Assignee: University of Kansas
    Inventors: Michael Detamore, Stevin H. Gehrke
  • Publication number: 20140113347
    Abstract: The present invention relates to a biopolymer composition for encapsulating cells, containing alginate, at least one glycosaminoglycan compound, preferably chondroitin sulfate, and at least one component of the extracellular matrix, preferably laminin, and to the process for producing the biopolymer composition. Also disclosed are a method for promoting cytoprotection using this composition, and the use of this composition for preparing a medicament useful in cell transplantation.
    Type: Application
    Filed: December 15, 2011
    Publication date: April 24, 2014
    Inventors: Ana Carolina Vale Campos Lisbôa, Gisella Grazioli, Ana Lúcia Campanha Rodrigues, Leticia Labriola, Mari Cleide Sogayar, Thiago Rennó Dos Mares Guia
  • Patent number: 8697058
    Abstract: The present invention relates to a method of making cytocompatible alginate gels and their use in the treatment of cardiomyopathy.
    Type: Grant
    Filed: September 20, 2012
    Date of Patent: April 15, 2014
    Assignee: Abott Cardiovascular Systems Inc.
    Inventors: Shubhayu Basu, Gene Michal, Florian N. Ludwig, Jinping Wan, John Stankus
  • Publication number: 20140072601
    Abstract: The present invention relates to hydrogels which may be used to encapsulate or entrap live cells. The invention further relates to methods for transporting live cells which are encapsulated or entrapped within hydrogels from a first location to a second location. The invention further relates to method of treating a wound, disease or tissue injury, e.g. an ocular injury or a damaged ocular surface in a subject using a hydrogel comprising corneal stem cells. The hydrogels used in such methods may be ones which have been transported from a first location to a second location.
    Type: Application
    Filed: March 21, 2012
    Publication date: March 13, 2014
    Inventors: Che John Connon, Richard Anthony Cave, Vitaliy Khutoryanskiy, Bernice Wright
  • Publication number: 20140017284
    Abstract: Macroporous 3-D tissue engineering scaffold comprising elastomeric cross-linked polymer units and interconnected macropores containing living cells are produced by step of: in a mixture of the cells and elastomeric polymer units comprising activatable crosslinking groups, activating the crosslinking groups to form the scaffold.
    Type: Application
    Filed: July 16, 2013
    Publication date: January 16, 2014
    Inventors: Fan Yang, Li-Hsin Han, Xinming Tong
  • Publication number: 20140012393
    Abstract: The present invention relates to a complex support body for regenerating bone-cartilage, a method for manufacturing thereof, and a composition for treating bone and cartilage related diseases comprising the same as an active ingredient, and more particularly, to a complex support body for regenerating bone-cartilage, which comprises a bone regeneration layer consisting of a biodegradable polymer and a biocompatible ceramic, and a cartilage regeneration layer, which is coupled to the bone regeneration layer and in which cells that can be differentiated into cartilages cells are fixed; a method for manufacturing thereof; and a composition for treating bone and cartilage related diseases comprising the same as an active ingredient.
    Type: Application
    Filed: February 20, 2012
    Publication date: January 9, 2014
    Inventors: Jung Woog Shin, Ji Won Shin, So Hee Park
  • Publication number: 20130344561
    Abstract: In some embodiments, the disclosure relates generally to methods as well as related compositions, systems, kits and apparatus comprising linking proteins to target compounds and/or to locations of interest using tethers. For example, the tether can used to link the protein to a target compound, for example, to link an enzyme to a substrate. Similarly, the tether can be used to link the protein at or near a desired location on a surface. In one group of embodiments, the tether includes a polynucleotide and the target compound or location on the surface includes another polynucleotide that is capable of hybridizing to the tether. In such bodiments, the tether can be used to link the protein to the target compound or location using nucleic acid hybridization.
    Type: Application
    Filed: February 8, 2012
    Publication date: December 26, 2013
    Inventors: John Davidson, Theo Nikiforov, Guobin Luo
  • Publication number: 20130337528
    Abstract: This invention relates generally to the fields of separation and conversion technologies, and more particularly to materials for use in tangential-flow filtration techniques. The tangential-flow materials are useful in a wide range of separation and conversion processes, including those reliant on reverse osmosis, microfiltration, ultrafiltration, or nanofiltration semipermeable filtration membranes, and provide efficient methods for purifying or producing various target substances, including biopolymer particles for use in tangential-flow filtration.
    Type: Application
    Filed: December 9, 2011
    Publication date: December 19, 2013
    Inventors: Tracy Thompson, Bernd Helmut Rehm, Andrew Brian Herbert, Edward George Saravolac
  • Publication number: 20130316431
    Abstract: The present application discloses immobilized enzymes and immobilized enzyme materials comprising a crosslinked organophosphate-degrading enzyme having a support material which includes a biomass material and/or a polymeric material. The resulting immobilized enzyme materials may be biodegradable. The present application also discloses methods of making and using the disclosed immobilized organophosphate hydrolase enzyme and enzyme materials.
    Type: Application
    Filed: November 13, 2012
    Publication date: November 28, 2013
    Inventors: Augustine A. DiNovo, Dominic P. DiNovo, David A. Schofield, Matthew F. Smiechowski, Francis H. Verhoff
  • Patent number: 8584892
    Abstract: A packet of an enzyme and/or enzyme producing bacteria is contained within the core of a roll of toilet tissue or similar paper product wound on a core. The packet has a cover that dissolves or disintegrates on contact with water and disperses its contents into the aqueous waste stream. The packet may contain a mixture of bacterial cultures that produce enzymes to attack the greasy or fatty components of the waste stream. An additional article such as a sample of a liquid or creme personal care product may also be contained within the tissue paper core.
    Type: Grant
    Filed: January 19, 2010
    Date of Patent: November 19, 2013
    Inventor: Joseph R. Lira
  • Publication number: 20130295637
    Abstract: The present invention relates to a method of releasably encapsulating pluripotent embryonic stem cells in a degradable continuous polyionic fiber for tissue culture, wherein the encapsulated embryonic stem cells are able to maintain a pluripotent phenotype in tissue culture; the method comprising (a) contacting an aqueous solution of a polyanion with an aqueous solution of a polycation to form an interface between the aqueous solution of polyanion and the aqueous solution of polycation, and wherein the aqueous solution of polyanion or the aqueous solution of polycation or both the aqueous solution of polyanion and the aqueous solution of polycation comprises a suspension of pluripotent embryonic stem cells; (b) drawing a continuous polyionic fiber which comprises encapsulated pluripotent embryonic stem cells from the interface; (c) passing the continuous polyionic fiber comprising encapsulated pluripotent embryonic stem cells in a continuous process through a solution which reduces secondary complexation of t
    Type: Application
    Filed: August 5, 2011
    Publication date: November 7, 2013
    Inventors: Hongfang Lu, Andrew Chwee Aun Wan, Jackie Y. Ying, Karthikeyan Narayanan
  • Patent number: 8569028
    Abstract: A method for encapsulation of microparticles (e.g., fungal conidia), involving (i) suspending microparticles in an aqueous solution containing at least one sugar to form an aqueous suspension wherein the concentration of said sugar is about 0.1 to 10% w/v, and (ii) spray drying said aqueous suspension with a spray dryer, wherein the inlet temperature of said spray dryer is about 40° C. to about 140° C. and the outlet temperature of said spray dryer is about 20° C. to about 80° C.
    Type: Grant
    Filed: June 9, 2010
    Date of Patent: October 29, 2013
    Assignee: The United States of America, as represented by the Secretary of the Agriculture
    Inventors: Xixuan Jin, Daniel B. Custis
  • Patent number: 8551522
    Abstract: A sustained-release formulation is provided comprising a solid dispersion composition comprising a tricyclic compound or a pharmaceutically acceptable salt thereof in a mixture comprising a water-soluble polymer and a water-insoluble polymer, and an excipient.
    Type: Grant
    Filed: November 5, 2008
    Date of Patent: October 8, 2013
    Assignee: Astellas Pharma Inc.
    Inventors: Kazunari Yamashita, Eiji Hashimoto, Yukihiro Nomura, Fumio Shimojo, Shigeki Tamura, Takeo Hirose, Satoshi Ueda, Takashi Saitoh, Rinta Ibuki, Toshio Ideno
  • Patent number: 8535923
    Abstract: Microcapsules including a capsule shell encapsulating a suspension of a therapeutically effective amount of liver cells in physical contact with a liver cell stimulating amount of erythropoietin.
    Type: Grant
    Filed: April 27, 2010
    Date of Patent: September 17, 2013
    Assignee: Cytonet GmbH & Co. KG
    Inventors: Krasimira Aleksandrova, Peter Pediaditakis, Jo Salisbury, Wolfgang Rüdinger