Carrier Is Carbohydrate Patents (Class 435/178)
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Patent number: 7423010Abstract: This invention provides novel methods and reagents for specifically delivering biologically active compounds to phagocytic mammalian cells. The invention also relates to specific uptake of such biologically active compounds by phagocytic cells and delivery of such compounds to specific sites intracellularly. The invention specifically relates to methods of facilitating the entry of antiviral and antimicrobial drugs and other agents into phagocytic cells and for targeting such compounds to specific organelles within the cell. The invention specifically provides compositions of matter and pharmaceutical embodiments of such compositions comprising conjugates of such antimicrobial drugs and agents covalently linked to particulate carriers generally termed microparticles. In particular embodiments, the antimicrobial drug is covalently linked to a microparticle via a cleavable linker moiety that is non-specifically cleaved in a phagocytic cell.Type: GrantFiled: February 22, 2005Date of Patent: September 9, 2008Assignee: Oregon Health & Science UniversityInventors: Milton Yatvin, Michael Stowell, Vincent Gallicchio, Michael Meredith
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Patent number: 7422737Abstract: The present invention provides cellular solid carriers comprising viable microorganisms capable of controlling plant pathogens. The cellular solid carriers are formed from water-soluble hydrocolloid beads dried under conditions which preserve their porosity, thereby allowing efficient release of microorganisms or diffusion of products derived from the microorganisms from the beads to the surrounding environment.Type: GrantFiled: September 5, 2003Date of Patent: September 9, 2008Assignee: Yissam Research Development Company of the Hebrew University of JerusalemInventors: Amos Nussinovitch, Ilan Chet, Cheinat Zohar Perz
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Patent number: 7417021Abstract: A biologically active conjugate is disclosed comprising a biopolymer and a therapeutic agent joined by a disulfide bond. The conjugate, when formulated in a pharmaceutical composition with a suitable carrier, has improved in vivo stability and activity, and can be targeted to a variety of cells, tissues and organs.Type: GrantFiled: May 7, 2004Date of Patent: August 26, 2008Assignee: Genzyme CorporationInventors: Pericles Calias, Robert J. Miller
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Publication number: 20080199914Abstract: Process for preparing a modified alginate polymers are disclosed. The processes comprise the steps of covalently attaching a modifying moiety to one or more unmodified monomeric subunits of an alginate polymer; and changing one or more unmodified mannuronic (M) monomeric subunits of the alginate polymer to one or more unmodified guluronic (G) monomeric sub-units by an enzymatic epimerization reaction performed in any order. Processes for preparing alginate gels, fiber, and compositions are also disclosed. Modified alginates in which only M monomeric subunits are modified, and alginate gels, fibers and compositions comprising the same, are disclosed.Type: ApplicationFiled: November 11, 2005Publication date: August 21, 2008Applicant: FMC BIOPOLYMER ASInventors: Gudmund Skjak-Braek, Ivan Donati
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Publication number: 20080193965Abstract: Methods of binding and detecting a microorganism on a solid substrate. The microorganism is bound on a solid substrate covalently bound to a capture agent having a saccharide moiety. A lectin capable of binding to the microorganism and the saccharide moiety of the capture agent is added to the sample to bind the microorganism on the solid substrate. Further provided are biosensor devices, such as a quartz crystal microbalance (QCM) device or a surface plasmon resonance (SPR) device, that incorporate the solid substrate for the detection of microorganisms.Type: ApplicationFiled: October 9, 2007Publication date: August 14, 2008Applicant: Oakland UniversityInventors: Xiangqun Zeng, Zhihong Shen
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Patent number: 7354750Abstract: The present invention provides compositions and methods for the separation of metals or molecules such as polypeptides, nucleic acids, or endotoxins using a metal-modified solid support. The metals or molecules are isolated from a starting material using the modified solid supports of the invention. Also provided by the invention are kits that can be used in connection with the inventive methods.Type: GrantFiled: October 20, 2003Date of Patent: April 8, 2008Assignee: Promega CorporationInventors: Daniel J. Simpson, Tonny Johnson, John Shultz, Roderick G. Flemming, Rebecca Godat, Sanchayita Kar, Robin Hurst
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Patent number: 7354733Abstract: We disclose methods of sorting or separating mixtures of living cells (e.g., eukaryotic, prokaryotic, mammalian, pathogenic, bacterial, viral, etc.). We perform our methods by activating cell-selective photophoric labels, which photosensitize and chemically reduce a photosensitive metal compound to form metal grains, particles or crystals. The metal adheres to the cells and forms the basis for sorting or separating different cell types. Photophoric labels may include chemiluminescent agents such as peroxidase enzymes activated with peroxidase substrates capable of luminescence. Photosensitive metal compounds may be present in a light-sensitive matrix or emulsion containing photosensitizable metal compounds, which form metal grains, particles or crystals upon exposure to a developer solution. Developer solutions are formulated to substantially allow living cells to remain viable after exposure to the developing solution.Type: GrantFiled: March 26, 2002Date of Patent: April 8, 2008Assignee: Cellect Technologies Corp.Inventors: Shmuel Bukshpan, Gleb Zilberstein
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Publication number: 20080032371Abstract: TAL cell biocatalyst was immobilized in alginate cross-linked beads using low concentrations of glutaraldehyde. The biocatalyst beads have highly stable TAL activity and mechanical strength such that they withstand prolonged recycling in production of pHCA.Type: ApplicationFiled: July 12, 2006Publication date: February 7, 2008Inventors: Robert Dicosimo, Sharon L. Haynie, Lixuan Lisa Huang, Fateme Sima Sariaslani, Robert J. Trotman, Tina K. Van Dyk
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Patent number: 7326324Abstract: Gellan can be purified from nucleic acid contamination by combining the contaminated gellan with DNase under conditions that allow the DNase to degrade the nucleic acid contaminant. The purified gellan is useful in gel electrophoresis. A buffer which allows cystamine to be used as a reversible cross-linker does not have to be recirculated during the course of a normal gel run.Type: GrantFiled: November 20, 2003Date of Patent: February 5, 2008Assignee: Shin-Etsu Chemical Co., Ltd.Inventors: Linda Thorne, Richard W Armentrout
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Patent number: 7304015Abstract: The present invention provides compositions for the separation of metals or biomolecules such as polypeptides, nucleic acids, or endotoxins using modified solid supports.Type: GrantFiled: August 11, 2005Date of Patent: December 4, 2007Assignee: Promega CorporationInventors: Daniel J. Simpson, Tonny Johnson, Roderick G. Flemming
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Patent number: 7304016Abstract: The present invention provides compositions for the separation of metals or biomolecules such as polypeptides, nucleic acids, or endotoxins using modified solid supports.Type: GrantFiled: May 19, 2006Date of Patent: December 4, 2007Assignee: Promega CorporationInventors: Daniel J. Simpson, Tonny M. Johnson, Roderick G. Flemming
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Patent number: 7300779Abstract: Granules that include a protein core are described. The protein core includes a protein matrix which includes a protein mixed together with a starch. The protein matrix can be layered over a seed particle or the protein core can be homogeneous. The protein can be an enzyme or a therapeutic protein.Type: GrantFiled: September 13, 2004Date of Patent: November 27, 2007Assignee: Genencor International, Inc.Inventors: Nathaniel T. Becker, Thomas S. Green
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Patent number: 7297331Abstract: Compositions of matter are described which contain restricted cancer cells. When so restricted, the cells produce an unexpectedly high amount of material which suppresses cancer cell proliferation. The phenomenon crosses cancer type and species lines. Processes for making these compositions, and their use, are also described.Type: GrantFiled: August 16, 2004Date of Patent: November 20, 2007Assignee: The Rogosin InstituteInventors: Shirin Asina, Kanti Jain, Albert L. Rubin, Barry Smith, Kurt Stenzel
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Patent number: 7294501Abstract: A use of an inoculum of a geldanamycin-producing strain able to survive in a plant rhizosphere as a biocontrol of common scab affecting the plant and a method for biocontrolling common scab comprising the use of such strain. A biologically pure culture of a Streptomyces strain deposited at the American Type Culture Collection (ATCC) Accession number BAA-668, or a variant thereof.Type: GrantFiled: December 8, 2003Date of Patent: November 13, 2007Assignee: Universite De SherbrookeInventors: Carole Beaulieu, Julie Beauséjour, Sonya Agbessi
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Patent number: 7276232Abstract: The present invention relates to compositions containing different stages of pathogenic protozoans in a gel and to their use for controlling rodents.Type: GrantFiled: May 4, 2001Date of Patent: October 2, 2007Assignee: Bayer CropScience AGInventors: Jochen Kalbe, Thomas Böcker, Stefan Endepols, Thomas Jäkel, Sermasakdi Hongnark
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Patent number: 7270829Abstract: A meat product containing in vitro produced animal cells in a three dimensional form and a method for producing the meat product.Type: GrantFiled: May 9, 2005Date of Patent: September 18, 2007Inventor: Willem Frederik Van Eelen
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Patent number: 7238505Abstract: The present invention relates to a DNA polymerase immobilized by covalent bonding. More particularly, the present invention relates to an immobilized DNA polymerase whose activity is maximally preserved by masking the active site of the DNA polymerase and optimizing interaction of the masked molecule to the substrate material. In one embodiment, the average activity of the immobilized DNA polymerase is more than about 10% relative to that of the solution phase DNA polymerase. Further provided by the invention are methods and kits for performing polymerase chain reactions (PCR).Type: GrantFiled: April 2, 2003Date of Patent: July 3, 2007Assignee: Ahram Biosystems Inc.Inventors: Hyun Jin Hwang, Jeong Hee Kim
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Patent number: 7183073Abstract: A device and method are provided for isolating and culturing microorganisms from a bulk fluid sample. The device comprises a container having therein a polymeric immobilization layer having interstitial spaces between polymer chains such as a gel matrix. The interstitial spaces are of an average size less than an average size of microorganisms to be separated from the sample and cultured. A bulk fluid sample is applied to the immobilization layer where fluid is absorbed by the layer and microorganisms remain on the surface of the layer. After culturing, microorganism colonies are readily accessible on the surface of the layer for harvest and testing. The immobilization layer may contain one or more of nutrients for microorganisms growth, lytic agents, lytic enzymes, antibiotics, antibiotic neutralizers, indicators, detergents and selective agents. An adjacent support layer may be above and/or below the immobilization layer.Type: GrantFiled: May 2, 2003Date of Patent: February 27, 2007Assignee: bioMerieux, Inc.Inventors: Jones M. Hyman, Paul M. Matsumura, Scott R. Jeffrey, Martin J. Maresch, Thurman C. Thorpe
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Patent number: 7153519Abstract: Water soluble macromers are modified by addition of free radical polymerizable groups, such as those containing a carbon-carbon double or triple bond, which can be polymerized under mild conditions to encapsulate tissues, cells, or biologically active materials. The polymeric materials are particularly useful as tissue adhesives, coatings for tissue lumens including blood vessels, coatings for cells such as islets of Langerhans, coatings, plugs, supports or substrates for contact with biological materials such as the body, and as drug delivery devices for biologically active molecules.Type: GrantFiled: June 25, 2003Date of Patent: December 26, 2006Assignee: Board of Regents, The University of Texas SystemInventors: Jeffrey A. Hubbell, Chandrashekhar P. Pathak, Amarpreet S. Sawhney, Neil P. Desai, Jennifer L. Hill, Syed F. A. Hossainy
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Patent number: 7122176Abstract: The present invention utilizes the pre-sporulation (preconidial) mycelial stage of entomopathogenic fungi as insect attractants and/or pathogens. The fungus can be cultivated on grain, wood, agricultural wastes or other cellulosic material, attracting the insect and optionally introducing insect-specific pathogenic fungi. More than one fungus and substrate can be used in combination. The matrix of preconidial fungi can optionally be dried, freeze-dried, cooled and/or pelletized and packaged and reactivated for use as an effective insect attractant and/or biopesticide. Attractant extracts of the preconidial entomopathogenic mycelium are disclosed.Type: GrantFiled: October 1, 2001Date of Patent: October 17, 2006Assignee: Mycosys, LLCInventor: Paul Edward Stamets
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Patent number: 7112453Abstract: This invention provides methods of retentate chromatography for resolving analytes in a sample. The methods involve adsorbing the analytes to a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.Type: GrantFiled: August 5, 2002Date of Patent: September 26, 2006Assignee: Ciphergen Biosystems, Inc.Inventors: T. William Hutchens, Tai-Tung Yip
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Patent number: 7105339Abstract: This invention provides methods of retentate chromatography for resolving analytes in a sample. The methods involve adsorbing the analytes to a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.Type: GrantFiled: July 23, 2003Date of Patent: September 12, 2006Assignee: Ciphergen Biosystems, Inc.Inventors: T. William Hutchens, Tai-Tung Yip
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Patent number: 7087728Abstract: The present invention relates to the field of carbohydrate crosslinked glycoprotein crystals. Advantageously, such crosslinked glycoprotein crystals display stability to harsh environmental conditions, while maintaining the structural and functional integrity of the glycoprotein backbone. According to one embodiment, this invention relates to methods for concentrating proteins that have been modified by carbohydrates and for releasing their activity at controlled rates. This invention also provides methods for producing carbohydrate crosslinked glycoprotein crystals and methods for using them in pharmaceutical formulations, vaccines, immunotherapeutics, personal care compositions, including cosmetics, veterinary pharmaceutical compositions and vaccines, foods, feeds, diagnostics, cleaning agents, including detergents and decontamination formulations.Type: GrantFiled: December 23, 2002Date of Patent: August 8, 2006Assignee: Altus Pharmaceuticals Inc.Inventors: Alexey L Margolin, Chandrika P Govardhan, Kalevi J Visuri, Sinikka S Uotila
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Patent number: 7083936Abstract: A composition containing polydisperse aminodextran polymer molecules is soluble in an aqueous solution at a concentration of 10 mg/ml. The molecules therein have a narrow size distribution characterized by an average molecule mean hydrodynamic diameter of greater than 115 nm, a polydispersity index of between 0.10 and 0.47, an average molecular weight (MW) greater than 3 million daltons, and an amine content of greater than 50 amines per molecule. Similar soluble compositions contain the polymer molecules with an average MW of greater than 7 million daltons. These compositions are useful in forming reagents by conjugation with proteins for labeling cells. Methods of making these compositions and reagents from conventional mixtures of aminodextran polymers involve fractionation on column chromatography.Type: GrantFiled: August 1, 2003Date of Patent: August 1, 2006Assignee: Beckman Coulter, Inc.Inventors: Stephen L. Ledis, Olavi Siiman, Cynthia G. Healy
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Patent number: 7070941Abstract: The invention provides methods and compositions for azide tagging of biomolecules. In one embodiment of the invention, proteins are tagged by metabolic incorporation of prenylated azido-analog substrates. Examples of such analogs are azido farnesyl diphosphate and azido farnesyl alcohol. The azido moiety in the resulting modified proteins provides an affinity tag, which can be chemoselectively captured by an azide-specific conjugation reaction, such as the Staudinger reaction, using a phosphine capture reagent. When the capture agent is biotinylated, the resulting conjugates can be detected and affinity-purified by streptavidin-linked- HRP and streptavidin-conjugated agarose beads, respectively. The invention allows detection and isolation of proteins with high yield, high specificity, and low contamination without harsh treatment of proteins.Type: GrantFiled: November 17, 2003Date of Patent: July 4, 2006Assignee: Board of Regents, The University of Texas SystemInventors: Yingming Zhao, John R. Falck
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Patent number: 7041504Abstract: Compositions of matter are described which contain restricted cancer cells. When so restricted, the cells produce an unexpectedly high amount of material which suppresses cancer cell proliferation. The phenomenon crosses cancer type and species lines. Processes for making these compositions, and their use, are also described.Type: GrantFiled: December 12, 2000Date of Patent: May 9, 2006Assignee: The Rogosin InstituteInventors: Shirin Asina, Kanti Jain, Albert L. Rubin, Barry Smith, Kurt Stenzel
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Patent number: 7026166Abstract: The present invention relates to the use of fluorogenic or chromogenic dyes as reporter molecules for detecting cell entry by a specific molecule.Type: GrantFiled: January 21, 2003Date of Patent: April 11, 2006Assignee: Chiron CorporationInventors: Daniel J. Suich, Ronald N. Zuckermann
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Patent number: 7018821Abstract: The present invention provides highly impact-resistant, water-soluble or water dispersible, low-dust granules comprising an active ingredient and methods for obtaining the same.Type: GrantFiled: June 20, 2002Date of Patent: March 28, 2006Assignee: Genencor International, Inc.Inventors: Nathaniel T. Becker, Mark S. Gebert, Isabelle Mazeaud
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Patent number: 7008634Abstract: Described are compositions with tethered growth effector molecules, and methods of using these compositions for growing cells and tissues. Growth effector molecules, including growth factors and extracellular matrix molecules, are flexibly tethered to a solid substrate. The compositions can be used either in vitro or in vivo to grow cells and tissues. By tethering the growth factors, they will not diffuse away from the desired location. By making the attachment flexible, the growth effector molecules can more naturally bind to cell surface receptors. A significant feature of these compositions and methods is that they enhance the biological response to the growth factors. The method also offers other advantages over the traditional methods, in which growth factors are delivered in soluble form: (1) the growth factor is localized to a desired target cell population; (2) significantly less growth factor is needed to exert a biologic response.Type: GrantFiled: March 3, 1995Date of Patent: March 7, 2006Assignee: Massachusetts Institute of TechnologyInventors: Linda G. Cima, Edward W. Merrill, Philip R. Kuhl
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Patent number: 6987022Abstract: An efficient method for therapeutic treatment of a leukemic patient is disclosed in which the patient's body fluid under external circulation is brought into direct contact with an adsorbent material capable of adsorbing the leukemic cells in the body fluid specifically and selectively. The leukemic cell-adsorbent material, which is used by filling a column to form an adsorbent bed, is a conjugate of a lectin protein extracted from, e.g., Dolichos beans or soybeans coupled with a physiologically inert carrier material such as a polysaccharide in the form of beads or a superparamagnetic material in the form of iron oxide-based magnetic beads.Type: GrantFiled: May 31, 2002Date of Patent: January 17, 2006Assignee: National Institute of Advanced Industrial Science and TechnologyInventors: Osamu Nakamura, Hideki Ohba, Imre Sallay, Fumio Yagi, Sawako Moriwaki, Seiji Yasuda
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Patent number: 6955886Abstract: The invention provides a scintillation proximity assay for detecting peptidoglycan synthesis. The assay is especially suitable for high throughput screening of compounds affecting peptidoglycan synthesis.Type: GrantFiled: May 4, 1999Date of Patent: October 18, 2005Assignee: AstraZeneca ABInventors: Sunita Desousa, Dwarakanath Prahlad
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Patent number: 6951745Abstract: A method for manufacturing polyhydroxyalkanoate-containing structure, at least a part of a base material surface of the structure being coated with polyhydroxyalkanoate, the method comprises the steps of immobilizing a polyhydroxyalkanoate synthase on the base material surface, synthesizing, on the base material surface, polyhydroxyalkanoate using a 3-hydroxyacyl coenzyme A to become the substrate of the synthase and the synthase and coating at least a part of the base material surface with the synthesized polyhydroxyalkanoate, wherein the synthase contains an amino acid sequence capable of binding to the base material.Type: GrantFiled: July 10, 2002Date of Patent: October 4, 2005Assignee: Canon Kabushiki KaishaInventors: Tsuyoshi Nomoto, Tetsuya Yano, Shinya Kozaki, Tsutomu Honma
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Patent number: 6939681Abstract: The invention relates to a method of evaluating the immunological status of a subject comprising the steps of 1) determining the content of an antibody in a liquid sample from the subject using an immunoassay, wherein the reaction between the antibody of the sample and a ligand in the form of an antigen, an antibody or a hapten, the ligand being directed to the Fab region of the sample antibody, is carried out in the presence of other constituents of the sample to obtain a measurement 1, 2) determining the content of an antibody in the liquid sample using an immunoassay, wherein the reaction between the antibody of the sample and a ligand in the form of an antigen, an antibody or a hapten, the ligand being directed to the Fab region of the sample antibody, is carried out in the absence of other constituents of the sample to obtain a measurement 2, and 3) interrelating measurements 1 and 2 to express the interference and using the interference as a parameter for evaluating the immunological status of the subjeType: GrantFiled: February 28, 2002Date of Patent: September 6, 2005Assignee: Alk-Abello A/SInventors: Hans-Henrik Ipsen, Niels Johansen, Rikke Morkeberg, Soren Bogestrand, Tine Charlotte Beck
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Patent number: 6926888Abstract: Encapsulated producer cells which are capable of expressing a molecule which is an inhibitor of CNS tumour growth provide a novel approach to the treatment of tumours, such as brain tumors which are localized within the central nervous system.Type: GrantFiled: August 25, 1999Date of Patent: August 9, 2005Assignee: FMC Biopolymer ASInventor: Rolf Bjerkvig
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Patent number: 6916651Abstract: The heated support can be manufactured by subjecting an immobilized microorganism support in which activated sludge is immobilized by entrapment to heat treatment. Thus, it is possible to support particular microorganisms at high concentrations in an immobilizing material without pure culturing of the microorganisms.Type: GrantFiled: February 7, 2002Date of Patent: July 12, 2005Assignee: Hitachi Plant Engineering & Construction Co., Ltd.Inventor: Tatsuo Sumino
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Patent number: 6897328Abstract: A single stage process for deacidifying glycerides involving: (a) providing a glyceride having an acid value of from about 5 to 20; (b) providing a lower alcohol; (c) providing an enzyme catalyst immobilized on a support material; and passing a mixture of (a) and (b), over (c), thereby deacidifying the glycerides.Type: GrantFiled: November 21, 2002Date of Patent: May 24, 2005Assignee: Cognis Deutschland GmbH & Co. KGInventors: Bernhard Gutsche, Albrecht Weiss, Ralf Otto, Tycho Michel
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Patent number: 6893850Abstract: The present invention provides a masking system for selectively applying cells to predetermined regions of a surface. A mask is positioned adjacent to a surface to cover some portions of the surface while allowing other portions of the surface to remain uncovered. Cells then are applied to uncovered portions of the surface and the mask removed. Alternatively, a cell-adhesion promoter is applied to uncovered portions of the surface, and then cells are applied to the surface before or after removal of the mask from the surface. The masking system can be pre-coated, at least on those surfaces which will come into contact with cells, with a cell-adhesion inhibitor to resist absorption of cells and thereby avoid cell damage when the mask is removed (if cells are deposited prior to removal of the mask). A polymeric elastomeric mask that comes into cohesive-conformal contact with a surface to be patterned can be used.Type: GrantFiled: March 15, 2001Date of Patent: May 17, 2005Assignee: President and Fellows of Harvard CollegeInventors: Emanuele Ostuni, Ravi Kane, George M. Whitesides, Rebecca J. Jackman, David C. Duffy
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Patent number: 6884433Abstract: Sustained release formulation containing tacrolimus or its hydrate is provided. The time (T63.2%) required for 63.2% of the maximum amount of tacrolimus or its hydrate to be dissolved is 0.7 to 15 hours. The time is measured in accordance to the Japanes Pharmacopocia, the 13-th edition, Dissolution Test, No. 2 (Puddles method, 50 rpm) using an aqueous 0.005% hydroxypropyl cellulose solution. This aqueous test solution is adjusted to pH 4.5, accordingly. The formulation further comprises a solid base which is a water-soluble or water-insoluble polymer. The formulation is in the form of a powder, fine powder, granule, tablet or capsule. The formulation is administered to a patient once a day for preventing organ or tissue rejection by transplantation or autoimmune disease. In addition, a solid dispersion composition is provided. The solid dispersion comprises tacrolimus or its hydrate in a mixture containing water-soluble or water-insoluble polymer and an excipient.Type: GrantFiled: April 14, 2003Date of Patent: April 26, 2005Assignee: Fujisawa Pharmaceutical Co., Ltd.Inventors: Kazunari Yamashita, Eiji Hashimoto, Yukihiro Nomura, Fumio Shimojo, Shigeki Tamura, Takeo Hirose, Satoshi Ueda, Takashi Saitoh, Rinta Ibuki, Toshio Ideno
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Patent number: 6884597Abstract: A method for simply and conveniently detecting acetyltransferase and deacetylase activities of proteins by executing an acetylation reaction of a peptide substrate with an acetyltransferase, or a deacetylation reaction of an acetylated peptide substrate with a deacetylase, and after the completion of these reactions, detecting the acetyl group bound to the peptide substrate by using an anti-acetylated peptide antibody. This system for detecting acetyltransferase and deacetylase activities using the anti-acetylated peptide antibody enables screening inhibitors or enhancers of acetyltransferase and deacetylase. A system for screening deacetylase inhibitors or acetyltransferase enhancers using cultured cells is also provided.Type: GrantFiled: July 18, 2000Date of Patent: April 26, 2005Assignee: Medical & Biological Laboratories, Co., Ltd.Inventors: Yoichi Taya, Katsuyuki Tamai, Toshiaki Miyazaki
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Patent number: 6884628Abstract: Multifunctional, polyionic copolymers with molecular architectures and properties optimized for specific applications are synthesized on/or applied to substrate surfaces for analytical and sensing purposes. The coatings are particularly useful for suppression of non-specific interaction, adsorption or attachment of molecular or ionic components present in an analyte solution. Chemical, biochemical or biological groups that are able to recognize, interact with and bind specifically to target molecules in the material containing the analyte to be detected can be coupled to, integrated into, or absorbed to the multifunctional copolymers. These multifunctional copolymer coatings are compatible with a variety of different established methods to detect, sense and quantify the target molecule in an analyte.Type: GrantFiled: April 28, 2000Date of Patent: April 26, 2005Assignees: Eidgenossische Technische Hochschule Zurich, Universitat ZurichInventors: Jeffrey A. Hubbell, Marcus Textor, Donald L. Elbert, Stephanie Finken, Rolf Hofer, Nicholas D. Spencer, Laurence Ruiz-Taylor
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Patent number: 6869784Abstract: Enzymes are modified by incorporating anchor sites for linking the enzymes to a target surface without destroying the catalytic activity of the enzymes. A stable carrier to accommodate and bind the selected enzyme is constructed, and the enzyme is non-covalently linked to the carrier, generally through metal salts of iminodiacetate.Type: GrantFiled: November 29, 2000Date of Patent: March 22, 2005Assignee: The United States of America as represented by the Secretary of AmericaInventors: Alok Singh, Mehran Pazirandeh, Paul E. Schoen, Michael A. Markowitz, J. Matthew Mauro
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Patent number: 6858582Abstract: Methods and reagents are provided for specifically targeting biologically active compounds such as antiviral and antimicrobial drugs, or prodrugs containing the biologically active compound to specific sites such as specific organelles in phagocytic mammalian cells. The biologically active compound or prodrug is linked to a microparticle with a linker that is non-specifically or specifically cleaved inside a phagocytic mammalian cell. Alternatively, the biologically active compound or prodrug is impregnated into a porous microparticle or coated on a nonporous microparticle, and then coated with a coating material that is non-specifically or specifically degraded inside a phagocytic mammalian cell. The prodrug contains the biologically active compound linked to a polar lipid such as ceramide with a specific linker such as a peptide that is specifically cleaved to activate the prodrug in a phagocytic mammalian cell infected with a microorganism.Type: GrantFiled: January 15, 2002Date of Patent: February 22, 2005Assignee: Oregon Health and Sciences UniversityInventors: Milton B. Yatvin, Michael HB Stowell, Vincent S. Gallicchio, Michael J. Meredith
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Patent number: 6855343Abstract: The present invention relates to a novel method useful for fast separation and quick identification of active ingredients in herbs. The separation procedures are efficient without tedious and time-consuming works by using chromatographies or HPLC. The present invention further relates to a method for ascertaining the quality of herbs by applying treated plastic slides on quality control of herbal fractions, based on identification of their biological activities.Type: GrantFiled: May 21, 2002Date of Patent: February 15, 2005Assignee: Advanced Gene Technology, CorpInventors: Li-Wei Hsu, Su-Chen Chang
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Patent number: 6835390Abstract: A non-human tissue engineered meat product and a method for producing such meat product are disclosed. The meat product comprises muscle cells that are grown ex vivo and is used for food consumption. The muscle cells may be grown and attached to a support structure and may be derived from any non-human cells. The meat product may also comprise other cells such as fat cells or cartilage cells, or both, that are grown ex vivo together with the muscle cells.Type: GrantFiled: November 16, 2001Date of Patent: December 28, 2004Inventor: Jon Vein
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Patent number: 6821107Abstract: Multiple cell layers are formed with one cell layer formed on another cell layer. A carrier is provided having an alginate gel layer formed on a porous membrane. An extracellular matrix component gel layer or extracellular matrix component sponge layer may be formed on the alginate gel layer. A cell layer is formed on the alginate gel layer, or the extracellular matrix component gel layer or extracellular matrix component sponge layer. The alginate gel layer is solubilized such as with a chelating agent to exfoliate the cell layer from the porous membrane, and the exfoliated cell layer is placed on another cell layer on a carrier. The number of cell layers formed on each other may be 1-10, preferably 1-5, and more preferably 1-3.Type: GrantFiled: October 27, 2000Date of Patent: November 23, 2004Assignee: Secretary of Agency of Industrial Science and TechnologyInventors: Masayuki Hara, Jun Miyake, Ayako Yamaki
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Patent number: 6818230Abstract: Compositions of matter are described which contain restricted cancer cells. When so restricted, the cells produce an unexpectedly high amount of material which suppresses cancer cell proliferation. The phenomenon crosses cancer type and species lines. Processes for making these compositions, and their use, are also described.Type: GrantFiled: January 3, 2003Date of Patent: November 16, 2004Assignee: The Rogosin InstituteInventors: Shirin Asina, Kanti Jain, Albert L. Rubin, Barry Smith, Kurt Stenzel
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Patent number: 6812000Abstract: This invention relates to a bioprocess engineering solution for a product removal process for use in a biofermentation. The invention discloses a process for withdrawing an aliquot of broth from a biofermentation vessel during at least a portion of the biofermentation, removing biocatalyst and water, chromatographically separating biofermentation products from the withdrawn broth using water as an eluent, and returning the remaining components of the broth back to the biofermentation vessel. Process chromatography permits highly selective separation of the target molecule, preventing feedback inhibition of the biofermentation.Type: GrantFiled: April 22, 2002Date of Patent: November 2, 2004Assignee: E. I. du Pont de Nemours and CompanyInventors: Antoinette E. Wilkins, David J. Lowe
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Patent number: 6808705Abstract: Compositions of matter are described which contain restricted cancer cells. When so restricted, the cells produce an unexpectedly high amount of material which suppresses cancer cell proliferation. The phenomenon crosses cancer type and species lines. Processes for making these compositions, and their use, are also described.Type: GrantFiled: January 3, 2003Date of Patent: October 26, 2004Assignee: The Rogosin InstituteInventors: Shirin Asina, Kanti Jain, Albert L. Rubin, Barry Smith, Kurt Stenzel
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Patent number: 6790652Abstract: A method and apparatus for screening an array of test compounds for bioactivity by contacting an array of test compounds with a detector layer capable of detecting bioactivity, and detecting a detector layer response. The detector layer is comprised of physiologically viable cells. The method and apparatus allow a large number of test compounds to be simultaneously assayed in parallel without the need for complex fluidic devices.Type: GrantFiled: January 8, 1999Date of Patent: September 14, 2004Assignee: BioImage A/SInventors: Bernard Robert Terry, Kurt Marshall Scudder, Per Olaf Gunnar Arkhammer, Ole Thastrup
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Patent number: 6790643Abstract: Granules that include a protein core are described. The protein core includes a protein matrix which includes a protein mixed together with a starch and optionally sugar such as sucrose. The protein matrix can be layered over a seed particle or the protein core can be homogeneous. The protein can be an enzyme or a therapeutic protein. A barrier layer may surround the protein core, and a coating can be applied to the seed particle, the protein matrix and/or the barrier layer.Type: GrantFiled: June 25, 2002Date of Patent: September 14, 2004Assignee: Genencor International, Inc.Inventors: Nathaniel T. Becker, Thomas S. Green