Oxidoreductase (1. ) (e.g., Luciferase) Patents (Class 435/189)
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Publication number: 20130011871Abstract: In one non-limiting aspect, sterilizable reagent materials for diagnostic elements are provided. In other aspects, sterilized diagnostic elements and techniques for the production of the same are disclosed. In one embodiment, a sterilized diagnostic element includes a chemical detection reagent including at least one component that is sensitive to ionizing radiation. The sterilized diagnostic element is also mediator-free and the at least one component sensitive to ionizing radiation is present in a functional form in a proportion of ? 80% based on the total amount of the respective component in the diagnostic element before sterilization. In certain aspects, the at least one component sensitive to ionizing radiation includes one or both of an enzyme and a coenzyme. Other aspects include, but are not limited to, unique methods, techniques, products, systems and devices involving sterilizable reagent materials or sterilized diagnostic elements.Type: ApplicationFiled: June 11, 2012Publication date: January 10, 2013Inventors: Carina Horn, Nelli Steinke
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Patent number: 8349599Abstract: Described are multimeric oxidoreductase complexes which function in the enzymatic conversion of a carbon substrate. The complexes having a dehydrogenase subunit and a cytochrome C subunit. Also described are polynucleotides coding for the multimeric complexes and methods of use thereof.Type: GrantFiled: September 9, 2011Date of Patent: January 8, 2013Assignee: Danisco US Inc.Inventors: Robert M. Caldwell, M. Harunur Rashid, Fernando Valle
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Publication number: 20130005610Abstract: Genes and genetic variants in human genomes are disclosed which are useful, inter alia, as diagnostic biomarkers.Type: ApplicationFiled: October 19, 2010Publication date: January 3, 2013Applicant: Myriad Genetics, IncorporatedInventor: Susanne Wagner
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Publication number: 20130005666Abstract: The NF-E2-related factor 2 (Nrf2) is a key transcriptional regulator of antioxidant defense and detoxification. To directly monitor stabilization of Nrf2 we fused its Neh2 domain, responsible for the interaction with its nucleocytoplasmic regulator, Keap1, to firefly luciferase (Neh2-luciferase). It is shown herein that Neh2 domain is sufficient for recognition, ubiquitination and proteasomal degradation of Neh2-luciferase fusion protein. The novel Neh2-luc reporter system allows direct monitoring of the adaptive response to redox stress and classification of drugs based on the time-course of reporter activation. The novel reporter was used to screen a library of compounds to identify activators of Nrf2. The most robust and yet non toxic Nrf2 activators found—nordihydroguaiaretic acid, fisetin, and gedunin-induced astrocyte-dependent neuroprotection from oxidative stress via an Nrf2-dependent mechanism.Type: ApplicationFiled: June 29, 2012Publication date: January 3, 2013Applicant: CORNELL UNIVERSITYInventors: Rajiv RATAN, Irina GAZARYAN, Natalya A. SMIRNOVA
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Patent number: 8343744Abstract: Cytochrome P450 BM-3 from Bacillus megaterium was engineered using a combination of directed evolution and site-directed mutagenesis to hydroxylate linear alkanes regio- and enantioselectively using atmospheric dioxygen as an oxidant. Mutant 9-10A-A328V hydroxylates octane primarily at the 2-position to form S-2-octanol (40% ee). Another mutant, 1-12G, hydroxylates alkanes larger than hexane primarily at the 2-position, but forms R-2-alcohols (40-55% ee). These biocatalysts are highly active for alkane substrates and support thousands of product turnovers. These regio- and enantio-selectivities are retained in whole-cell biotransformations with E. coli, where the engineered P450s can be expressed at high levels and the expensive cofactor is supplied endogenously.Type: GrantFiled: January 3, 2011Date of Patent: January 1, 2013Assignee: The California Institute of TechnologyInventors: Frances H Arnold, Matthew W Peters, Peter Meinhold
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Publication number: 20120329131Abstract: A method for increasing the activity of superoxide dismutase comprises steps of: liquid culture, by providing a strain of Bacillus subtilis and a liquid medium to carry out a fed-batch fermentation and to obtain a primary fermentation broth, wherein the liquid medium has 8 wt % to 10 wt % of maltose, 10 wt % to 15 wt % of soya powder and rest of water; and solid culture, by culturing the primary fermentation broth in a solid medium, which has solid substrate and maltose in a ratio of 10:1 to 20:1, to obtain a secondary fermentation broth with SOD in high activity, wherein the solid substrate contains wheat germ and water.Type: ApplicationFiled: June 22, 2011Publication date: December 27, 2012Inventors: Pao-Chuan HSIEH, Wan-Ting Su
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Publication number: 20120329709Abstract: The present invention provides methods and materials by which glycosylation of glycoproteins can be regulated. Methods include the monitoring and regulation of parameters such that a glycoprotein having a desired product quality is obtained.Type: ApplicationFiled: May 25, 2010Publication date: December 27, 2012Inventors: Brian Edward Collins, Tiffany Guo, Lakshmanan Thiruneelakantapillai, Kevin Millea, Dorota A. Bulik
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Patent number: 8338152Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-8 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) and using these delta-8 desaturases in plants.Type: GrantFiled: April 18, 2012Date of Patent: December 25, 2012Assignee: E. I. du Pont de Nemours and CompanyInventors: Howard Glenn Damude, Quinn Qun Zhu
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Publication number: 20120322136Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl(1,3-oxazolidin-3-yl))-1-(4-fluorophenyl)pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize the intermediate (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one in a process for making Ezetimibe.Type: ApplicationFiled: August 21, 2012Publication date: December 20, 2012Applicant: CODEXIS, INC.Inventors: Emily Mundorff, Erik De Vries
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Publication number: 20120315629Abstract: Described are cell surface and circulating markers for aging related disorders (specific isoforms of NADH oxidase (arNOX)). Recombinant age-related NADH oxidase isoforms and their coding sequences and methods for detecting arNOX isoform presence and quantitation in tissues and in blood, sera, urine, saliva, perspiration and in other body fluids, are provided. Recombinant arNOX proteins are useful in preparing antigens for use in the generation of monoclonal and polyclonal antibodies as well as immunogenic compositions for diagnosis and treatment of aging disorders. DNA probes based on the DNA sequence information provide may be used to identify individuals at risk for aging disorders and for development of therapeutic interventions or anti-aging cosmetic or other formulations of benefit in slowing the aging process in mammals.Type: ApplicationFiled: August 17, 2010Publication date: December 13, 2012Applicant: Nox Technologies ,Inc.Inventors: D. James Morre, Xiaoyu Tang, Sara Dick, Christiaan Meadows, Dorothy M. Morre
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Patent number: 8329872Abstract: A method for obtaining at least one binding agent which binds a pharmaceutically active form of the compound with a higher specificity than a pharmaceutically inactive form of the compound is described by using special derivatives of said parent compound. The invention also pertains to the respectively created binding agents and derivatives. Furthermore, drug monitoring assays using said binding agents for monitoring pharmaceutically active forms of said parent compound are provided.Type: GrantFiled: November 7, 2011Date of Patent: December 11, 2012Assignee: Novartis AGInventors: Patrick Albientz, Jean-Michel Grenet, Rainer Hillenbrand, Francois Legay, Peter Marbach, Severine Marrony, Judith Schaefer
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Patent number: 8329438Abstract: The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.Type: GrantFiled: December 23, 2009Date of Patent: December 11, 2012Assignee: Codexis, Inc.Inventors: Christopher Savile, Vesna Mitchell, Xiyun Zhang, Gjalt Huisman
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Patent number: 8329439Abstract: A modified pyrroloquinoline quinone glucose dehydrogenase that exhibits a high selectivity for glucose is provided. A modified pyrroloquinoline quinone glucose dehydrogenase is disclosed in which the amino acid residue G at Position 99 of a pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) represented by SEQ ID NO: 1, or the amino acid residue G at Position 100 of the pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) represented by SEQ ID NO: 3, is substituted by the amino acid sequence TGZN (where Z is SX, S, or N and X is any amino acid residue). The modified PQQGDH of the present invention may additionally comprise one or more mutations selected from the group consisting of Q192G, Q192A, or Q192S; L193X; E277X; A318X; Y367A, Y367F, or Y367W; G451C; and N452X (where X is any amino acid residue).Type: GrantFiled: June 23, 2008Date of Patent: December 11, 2012Assignees: ARKRAY, Inc., Ultizyme International Ltd.Inventor: Koji Sode
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Patent number: 8323936Abstract: The invention relates to a process for the enantioselective enzymatic reduction of secodione derivatives. The secodione derivative is reduced with an oxidoreductase/dehydrogenase in the presence of NADH or NADPH as a cofactor. The secodione derivative is used in the reaction batch at a concentration of ?10 g/l and the oxidized cofactor NAD or NADP formed by the oxidoreductase/dehydrogenase is regenerated continuously.Type: GrantFiled: September 7, 2011Date of Patent: December 4, 2012Assignee: IEP GmbHInventors: Antje Gupta, Anke Tschentscher, Maria Bobkova
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Patent number: 8323924Abstract: Compositions and methods for producing aldehydes, alkanes, and alkenes are described herein. The aldehydes, alkanes, and alkenes can be used in biofuels.Type: GrantFiled: February 22, 2010Date of Patent: December 4, 2012Assignee: LS9, Inc.Inventors: Andreas Schirmer, Mathew Rude, Shane Brubaker
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Publication number: 20120301919Abstract: This invention relates to nucleic acid molecules comprising at least one nucleic acid sequence encoding for a peptide or protein of interest, at least one nucleic acid sequence encoding for a selectable marker, and at least one IRES sequence, wherein the at least one IRES sequence is located between the at least one nucleic acid sequence encoding for the peptide or protein of interest and the at least one nucleic acid sequence encoding for the selectable marker. Furthermore, this invention relates to host cells comprising such nucleic acid molecule and to methods of recombinant protein expression using such host cells.Type: ApplicationFiled: May 24, 2012Publication date: November 29, 2012Applicant: Agency for Science, Technology and ResearchInventors: Yuansheng Yang, Steven Ho, Jia Juan Lee
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Publication number: 20120295324Abstract: Methods, compositions, and systems for overproducing ligninolytic enzymes from the basidiomycetous fungus are described herein. As described, the method can include incubating a fungal strain of Cerrena unicolor IB303 in a fermentation system having growth medium which includes lignocellulosic material and then cultivating the fungal strain in the fermentation system under conditions wherein the fungus expresses the ligninolytic enzymes. In some cases, the lignocellulosic material is mandarin peel, ethanol production residue, walnut pericarp, wheat bran, wheat straw, or banana peel.Type: ApplicationFiled: May 4, 2012Publication date: November 22, 2012Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Vladimir Elisashvili, Eva Kachlishvili, Tamas Torok
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Publication number: 20120295252Abstract: A composition for analyzing nucleic acid that contains luciferase for which reactivity to dATP is equal to or less than 1/400 reactivity to ATP. A method for analyzing nucleic acid that comprises the use of the composition. A kit for analyzing nucleic acid comprising the composition. The method provides for an inexpensive, highly accurate and highly sensitive nucleic acid analysis that uses dATP instead of an expensive reagent having a low reactivity to DNA polymerase.Type: ApplicationFiled: June 20, 2011Publication date: November 22, 2012Applicant: Kikkoman CorporationInventors: Shigeya Suzuki, Yukako Kodama, Keiko Kurosawa, Eiry Kobatake, Masayasu Mie
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Publication number: 20120295326Abstract: Briefly described, embodiments of this disclosure include polynucleotides that encode mutant Cnidarian luciferases that exhibit modulated properties as compared to the corresponding wild-type luciferases, and the modulated properties include at least one of: modulated stability; enhanced light output; and modulated emission maximum. Embodiments of the present disclosure also include polypeptides or fragments thereof encoded by the polynucleotides, constructs including the polynucleotide, expression cassettes, cells, methods of producing the polynucleotides and polypeptides, antibodies, transgenic cells and/or animals, kits, and the like.Type: ApplicationFiled: July 31, 2012Publication date: November 22, 2012Applicants: The Board of Trustees of the Leland Stanford Junior University, THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Sanjiv S. Gambhir, Andreas M. Loening, Anna M. Wu
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Publication number: 20120295325Abstract: A firefly luciferase having the amino acid sequence of firefly luciferase, wherein the amino acid corresponding to position 287 of Heike firefly luciferase is replaced with alanine, or wherein the amino acid corresponding to position 392 is replaced with isoleucine. The firefly luciferase has improved thermostability and storage stability. A firefly luciferase gene coding for the firefly luciferase. By utilizing this gene, it is possible to efficiently produce firefly luciferase with increased stability. It is also possible to obtain firefly luciferase with further increased stability by combination with a mutation in which the amino acid at position 326 is replaced with serine, and/or a mutation in which the amino acid at position 467 is replaced with isoleucine.Type: ApplicationFiled: February 16, 2010Publication date: November 22, 2012Applicant: KIKKOMAN CORPORATIONInventors: Yukako Kodama, Eriko Yoshihara
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Patent number: 8313936Abstract: The present invention provides polypeptides involved in cryptophycin biosynthesis and the nucleic acid molecules that encode such polypeptides. The nucleic acid molecules and polypeptides of the invention or variants thereof can be used in the methods of the invention to produce cryptophycins.Type: GrantFiled: July 22, 2009Date of Patent: November 20, 2012Assignee: Regents of the University of MichiganInventors: David H Sherman, Zachary Q Beck, Yousong Ding
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Patent number: 8309328Abstract: The present application provides genetically modified fungal organisms that produce enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to glucose, enzyme mixtures produced by the genetically modified fungal organisms, and processes for producing glucose from cellulose using such enzyme mixtures.Type: GrantFiled: July 2, 2012Date of Patent: November 13, 2012Assignee: Codexis, Inc.Inventors: Ish Kumar Dhawan, Dipnath Baidyaroy, Andrew Shaw, Oleh Tanchak, Christopher Hill, Chengsong Liu, Amala Chokshi, Brian R. Scott
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Patent number: 8304224Abstract: The present invention relates a host cell comprising an expression vector comprising a nucleic acid molecule encoding a protein requiring gamma-carboxylation and associated expression control sequences and a nucleic acid molecule encoding a vitamin K epoxido reductase and associated expression control sequences and a nucleic acid molecule encoding a ?-glutamyl carboxylase and associated control sequences. The invention further relates to a method of producing a protein requiring gamma-carboxylation in high yields.Type: GrantFiled: June 23, 2011Date of Patent: November 6, 2012Assignee: MedImmune LimitedInventor: Ann Lövgren
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Publication number: 20120276247Abstract: The present invention relates to a newly identified lipase belonging to the Ustilaginaceae family of Basidomycetes and variants thereof. The invention also relates to polynucleotides encoding the lipase and the variants thereof. The invention further relates to procedures for producing the lipase, and variants thereof, polypeptides and polynucleotides. The invention further relates to lipase variants with an increased trans-selectivity. The invention further relates to lipase variants with a preference for long chain fatty acid esters or carboxylic acid moieties with a chain length greater than C12. The invention further relates to a method of reducing, or eliminating, trans-fatty acids from liquid oil stable enough for frying systems.Type: ApplicationFiled: April 29, 2011Publication date: November 1, 2012Inventors: Uwe Bornscheuer, Henrike Brundiek, Andrew Evitt, Stefan Sass, Friedericke Bönisch, Robert Kourist
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Publication number: 20120276610Abstract: The present invention relates to the isolation and characterization of a protein responsible for the reduction of uranium (VI) to uranium (IV). The present invention extends to the use of the isolated protein in the reduction of uranium (VI) to uranium (IV) and further extends to a process for the bioremediation, or at least partial remediation, of a site contaminated with a source of U (VI). According to a first aspect thereof, the present invention provides an isolated polypeptide derived from Thermus scotoductus strain SA-01 that is responsible for the reduction of uranium (VI), in a source of uranium (VI), to uranium (IV), wherein the polypeptide comprises the amino acid sequence of SEQ ID No: 1.Type: ApplicationFiled: September 21, 2010Publication date: November 1, 2012Applicant: University of the Free StateInventors: Errol Duncan Cason, Esta Van Heerden, Lizelle Ann Piater, Abitha Gyanendra Jugdave, Jacqueline Van Marwijk
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Publication number: 20120276145Abstract: A method of identifying a biofilm that includes non-typeable Haemophilus influenza (NTHi) including a step of screening a sample for the presence of one or more biofilm-specific proteins that are expressed by NTHi. In some cases, an NTHi biofilm-related disease in a subject is diagnosed. Also disclosed are protein microarrays for screening biofilm-specific proteins in a sample, formulations comprising one or more biofilm-specific proteins or fragments thereof, and methods for inducing an immune response in a patient against a biofilm-related infection.Type: ApplicationFiled: March 27, 2012Publication date: November 1, 2012Applicant: HOUSE RESEARCH INSTITUTEInventors: Paul Webster, Siva Wu, James Kerwin
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Publication number: 20120276567Abstract: The invention relates to a Talaromyces transformant comprising one or more recombinant gene, capable of producing cellulase in the absence of cellulase inducer in a glucose medium, having a cellulase activity of 2 WSU/ml or more, in 16 times or more diluted supernatant or broth.Type: ApplicationFiled: November 4, 2010Publication date: November 1, 2012Applicant: DSM IP ASSETS B.V.Inventors: Alrik Pieter Los, Brenda Vonk, Marco Alexander Berg Van Den, Robbertus Antonius Damveld, Cornelis Maria Jacobus Sagt, Adrianus Wilhelmus Hermanus Vollebregt, Margot Elisabeth Francoise Schooneveld-Bergmans
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Patent number: 8298812Abstract: The invention relates to yeast strains in which a human GLUT4 transport or a human GLUT1 transporter can be functionally expressed and to particular GLUT4 transport proteins which can be functionally expressed particularly readily in yeast strains.Type: GrantFiled: November 9, 2009Date of Patent: October 30, 2012Assignee: Sanofi-Aventis Deutschland GmbHInventors: Guenter Mueller, Silke Dlugai, Doerthe Voss, Eckhard Boles
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Publication number: 20120270285Abstract: Stereospecific carbonyl reductases SCR1, SCR2, and SCR3 are described herein as are nucleotide sequences that encode these reductases. These stereospecific carbonyl reductases have anti-Prelog selectivity and have specificities that are useful for fine biochemical synthesis.Type: ApplicationFiled: June 23, 2010Publication date: October 25, 2012Inventors: Gaetano T. Montelione, Rong Xiao, Yao Nie, Yan Xu
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Patent number: 8293228Abstract: The present invention relates to genetically modified proteins with uricolytic activity. More specifically, the invention relates to proteins comprising truncated urate oxidases and methods for producing them, including PEGylated proteins comprising truncated urate oxidases.Type: GrantFiled: April 20, 2012Date of Patent: October 23, 2012Assignee: Savient Pharmaceuticals Inc.Inventors: Jacob Hartman, Simona Mendelovitz
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Publication number: 20120264179Abstract: The invention provides a non-naturally occurring microbial organism having an adipate, 6-aminocaproic acid or caprolactam pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid or caprolactam pathway. The invention additionally provides a method for producing adipate, 6-aminocaproic acid or caprolactam. The method can include culturing an adipate, 6-aminocaproic acid or caprolactam producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an adipate, 6-aminocaproic acid or caprolactam pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid or caprolactam.Type: ApplicationFiled: April 20, 2012Publication date: October 18, 2012Applicant: GENOMATICA, INC.Inventors: Anthony P. Burgard, Priti Pharkya, Robin E. Osterhout
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Publication number: 20120266329Abstract: The invention provides polypeptides, including enzymes, structural proteins and binding proteins, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. Polypeptides, including enzymes and antibodies, and nucleic acids of the invention can be used in industrial, experimental, food and feed processing, nutritional and pharmaceutical applications, e.g., for food and feed supplements, colorants, neutraceuticals, cosmetic and pharmaceutical needs.Type: ApplicationFiled: February 20, 2012Publication date: October 18, 2012Applicant: BP Corporation North America Inc.Inventors: Eric J. Mathur, Cathy Chang
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Publication number: 20120264169Abstract: The present invention relates to cells producing at least one polypeptide of interest and expressing one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s), and methods for producing a polypeptide of interest essentially free from contaminating DNA, said method comprising the steps of: (a) cultivating a cell that produces at least one polypeptide of interest and expresses one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s); and (b) isolating the polypeptide of interest.Type: ApplicationFiled: June 25, 2012Publication date: October 18, 2012Applicant: NOVOZYMES A/SInventors: Michael Dolberg Rasmussen, Jon Martin Persson
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Patent number: 8288147Abstract: Methods for synthesizing isopentenyl pyrophosphate are provided. A first method comprises introducing into a host microorganism a plurality of heterologous nucleic acid sequences, each coding for a different enzyme in the mevalonate pathway for producing isopentenyl pyrophosphate. A related method comprises introducing into a host microorganism an intermediate in the mevalonate pathway and at least one heterologous nucleic acid sequence, each sequence coding for an enzyme in the mevalonate pathway necessary for converting the intermediate into isopentenyl pyrophosphate. The invention also provides nucleic acid sequences, enzymes, expression vectors, and transformed host cells for carrying out the methods.Type: GrantFiled: February 15, 2011Date of Patent: October 16, 2012Assignee: The Regents of the University of CaliforniaInventors: Jay D. Keasling, Vincent J.J. Martin, Douglas J. Pitera, Seon-Won Kim, Sydnor T. Withers, III, Yasuo Yoshikuni, Jack Newman, Artem Valentinovich Khlebnikov
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Patent number: 8288131Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of stereo specifically reducing (R)-2-methylpentanal to (R)-2-methylpentanol. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to produce (R)-2-methylpentanol and related compounds.Type: GrantFiled: August 21, 2009Date of Patent: October 16, 2012Assignees: Codexis, Inc., Pfizer, Inc.Inventors: Rama Voladri, Owen Gooding, Stephan Jenne, Emily Mundorff
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Patent number: 8288141Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine.Type: GrantFiled: August 27, 2009Date of Patent: October 16, 2012Assignee: Codexis, Inc.Inventors: Christopher Savile, John M. Gruber, Emily Mundorff, Gjalt Huisman, Steven James Collier
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Patent number: 8283146Abstract: The invention is related to processing enzyme comprising an N-terminally attached tag derived from highly basic proteins from thermophilic bacteria. The processing enzymes are useful for modifying proteins. They can be produced in high yields and can be effectively separated from the modified protein after use.Type: GrantFiled: May 17, 2012Date of Patent: October 9, 2012Assignee: Novo Nordisk Health Care AGInventor: Allan Christian Shaw
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Publication number: 20120252093Abstract: Promoter regions associated with the Yarrowia lipolytica esterase/lipase (EL1) gene are disclosed and have been found to be particularly effective for the expression of heterologous genes in yeast. These promoter regions will be useful for driving high-level expression of genes involved in the production of omega-3 and omega-6 fatty acids.Type: ApplicationFiled: March 30, 2012Publication date: October 4, 2012Applicant: E I Du Pont De Nemours and CompanyInventor: Quinn Qun Zhu
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Publication number: 20120252064Abstract: The present invention is related to a fungal serine protease enzyme, which said enzyme has serine protease activity and comprises an amino acid sequence of Malbranchea ALKO4122 mature protease as defined in SEQ ID NO:18 or an amino acid sequence having at least 66% identity to the amino acid sequence of SEQ ID NO:18. Also disclosed is an isolated nucleic acid molecule, comprising a polynucleotide sequence which encodes a fungal serine protease enzyme, nucleic acid sequences encoding said protease, a host cell and a process of producing a polypeptide having serine protease activity. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, wool, hair, leather, or silk, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.Type: ApplicationFiled: March 29, 2012Publication date: October 4, 2012Applicant: AB ENZYMES OYInventors: Leena Valtakari, Kari Juntunen, Marja Paloheimo, Pentti Ojapalo
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Publication number: 20120246760Abstract: Identification of a protein having an activity of oxidizing oleanane-type triterpene, and a gene encoding the protein, the protein and the gene, and use thereof are provided. For example, a protein having an activity of oxidizing oleanane-type triterpene obtained from a plant in the family Fabaceae, a gene encoding the protein and use thereof are provided. The protein is shown in, for example, SEQ ID NO: 4, 14 or 18, and the gene encoding the protein is shown in, for example, SEQ ID NO: 3, 13 or 17. A transformant into which the gene is introduced can be produced, and thereby a triterpene oxidase can be obtained.Type: ApplicationFiled: August 31, 2009Publication date: September 27, 2012Applicant: RIKENInventors: Toshiya Muranaka, Hikaru Seki, Kiyoshi Ohyama, Hiroshi Sudo, Satoru Sawai, Kazuki Saito
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Patent number: 8273562Abstract: A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.Type: GrantFiled: January 5, 2012Date of Patent: September 25, 2012Assignee: Ajinomoto Co., Inc.Inventors: Tomohiro Kodera, Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Yury Ivanovich Kozlov, Vitaly Grigorievich Paraskevov, legal representative, Sakayu Shimizu, Jun Ogawa, Makoto Hibi
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Patent number: 8273554Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl(1,3-oxazolidin-3-yl))-1-(4-fluorophenyl)pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize the intermediate (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one in a process for making Ezetimibe.Type: GrantFiled: August 20, 2009Date of Patent: September 25, 2012Assignee: Codexis, Inc.Inventors: Emily Mundorff, Erik De Vries
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Patent number: 8273547Abstract: The present disclosure provides ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, method of using the engineered ketoreductase enzymes to synthesize a variety of chirally pure compounds, and the chirally pure compounds prepared therewith.Type: GrantFiled: December 23, 2010Date of Patent: September 25, 2012Assignee: Codexis, Inc.Inventors: Lorraine Joan Giver, Lisa Marie Newman, Emily Mundorff, Gjalt W. Huisman, Stephane J. Jenne, Jun Zhu, Behnaz Behrouzian, John H. Grate, James Lalonde
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Publication number: 20120240248Abstract: The present invention relates to nucleic acids derived from Limonomyces roseipellis. The invention also relates to the individual coding sequences and to proteins encoded by these sequences as well to a process for converting oleic acid to linoleic acid to linoleic acid and the production of arachidonic acid, eicosapentaenoic acid and/or docosahexaenoic acid in a plant.Type: ApplicationFiled: November 23, 2010Publication date: September 20, 2012Applicant: BASF Plant Science Company GmbHInventors: Jörg Bauer, Xiao Qiu, Patricia Vrinten
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Publication number: 20120233716Abstract: The present invention relates to nucleic acids derived from Sphaeroforma arctica. The invention also relates to the individual coding sequences and to proteins encoded by these sequences in combination with other sequences as well as to a process for converting oleic acid to linoleic acid to linoleic acid and the production of arachidonic acid, eicosapentaenoic acid and/or docosahexaenoic acid in a plant.Type: ApplicationFiled: November 23, 2010Publication date: September 13, 2012Applicant: BASF Plant Science Company GmbHInventors: Jörg Bauer, Xiao Qiu, Patricia Vrinten
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Publication number: 20120231498Abstract: The invention is related to processing enzyme comprising an N-terminally attached tag derived from highly basic proteins from thermophilic bacteria. The processing enzymes are useful for modifying proteins. They can be produced in high yields and can be effectively separated from the modified protein after use.Type: ApplicationFiled: May 17, 2012Publication date: September 13, 2012Applicant: Novo Nordisk Health Care AGInventor: Allan Christian Shaw
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Patent number: 8263384Abstract: Novel organisms, including DNA construct host cell combinations, are disclosed. The organisms comprise a transcription unit (e.g. operon) comprising DNA sequences encoding for enzymes which promote the supply of single carbon units for the conversion of dUMP to dTMP. Examples include: dihydrofolate reductase genes e.g. T4 frd; Serine Hydroxymethyltransferase genes e.g. glyA; 3-phosphoglycerate dehydrogenase genes e.g. serA; and THF synthase genes e.g. ADE3. The organisms are used in a biological method of producing thymidine with significantly reduced levels of uridine.Type: GrantFiled: February 5, 2007Date of Patent: September 11, 2012Assignee: Glaxo Group LimitedInventors: David Martin Anderson, Andrew John Collis, Lin Liu, Sergey Podkovyrov, Christopher Preston
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Patent number: 8263326Abstract: Compositions and methods for modulating the activation of nuclear factor ?B (NF-?B) are provided. The compositions comprise one or more agents that modulate ubiquitination of phosphorylated I?B? and/or I?B?. Such compositions may be used for treating diseases associated with NF-?B activation. Modulating agents include human E3 ubiquitin ligases, antibodies thereto and variants thereof, as well as related proteins.Type: GrantFiled: August 8, 2008Date of Patent: September 11, 2012Assignee: Signal Pharmaceuticals, LLCInventors: Anthony M. Manning, Frank Mercurio, Sharon Amit, Yinon Ben-Neriah, Matti Davis, Ada Hatzubai, Avraham Yaron, Irit Alkalay, Aaron Ciechanover
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Patent number: 8263373Abstract: Embodiments of the invention relate to the microbial production of polyhydroxyalkanoic acids, or polyhydroxyalkanoates (PHA), from substrates which cannot be used as a source of carbon and/or energy for microbial growth or PHA synthesis and which have microbial and environmental toxicity. According to one embodiment of the invention, a process for the production of PHA is provided wherein an enzyme such as methane monooxygenase is used to convert volatile organic compounds into PHA through the use of microorganisms that are unable to use volatile organic compounds as a source of carbon for growth or PHA production.Type: GrantFiled: August 24, 2009Date of Patent: September 11, 2012Assignee: Newlight Technologies, LLCInventors: Markus Herrema, Kenton Kimmel
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Patent number: 8263376Abstract: The present disclosure relates to an isolated lipoxygenase. The lipoxygenase of the present disclosure includes polypeptide with at least 80% sequence homology to the mature peptide shown in SEQ ID NO: 2. Lipoxygenase of the present disclosure is suitable for use in, among other things, baking and in detergent compositions.Type: GrantFiled: March 31, 2011Date of Patent: September 11, 2012Assignee: Novozymes A/SInventors: Akiko Sugio, Shinobu Takagi