Oxidoreductase (1. ) (e.g., Luciferase) Patents (Class 435/189)
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Publication number: 20140272608Abstract: A multicopper oxidase mutant having improved resistance to imidazole compounds is provided. A multicopper oxidase mutant, which comprises an amino acid sequence derived from the amino acid sequence shown in SEQ ID NO: 2 by substitution of either or both an amino acid residue corresponding to methionine at position 157 and an amino acid residue corresponding to proline at position 414 with a different amino acid and has activity of catalyzing a reaction generating water molecules via four-electron reduction of oxygen molecules using ABTS (2,2?-azinobis(3-ethylbenzoline-6-sulfonate)) as a substrate is provided.Type: ApplicationFiled: October 5, 2012Publication date: September 18, 2014Inventors: Takahiro Kusumegi, Kumi Terada
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Patent number: 8835136Abstract: Non-naturally occurring amine dehydrogenases (AmDH) and methods of use thereof the produce chiral amines are disclosed. The AmDH are variants of amino acid dehydrogenases. AmDH based on phenylalanine, leucine, and valine scaffolds are provided. The AmDH typically have one, two, three, four, or more amino acid alterations relative to the scaffold. The alterations to the scaffold result in an enzyme that accepts the analogous ketone, such as methyl isobutyl ketone (MIBK), instead of the wild-type ?-keto acid. Chimeric AmDH are also disclosed. The chimeras are fusion proteins that include a substrate binding domain from a first AmDH and a cofactor binding domain from a second AmDH. In a preferred embodiment, one of the domains is from a PheDH-based AmDH and one of the domains is from a LeuDH-based AmDH.Type: GrantFiled: May 15, 2013Date of Patent: September 16, 2014Assignee: Georgia Tech Research CorporationInventors: Andreas Sebastain Bommarius, Michael Justin Abrahamson, Bettina Bommarius
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Publication number: 20140259213Abstract: The present invention relates to an isolated nucleic acid comprising a nucleotide sequence encoding a mutated HPPD protein, wherein said mutated HPPD protein has HPPD activity, wherein in said mutated HPPD protein at least one amino acid has been replaced so that the resulting amino acid sequence comprises at least one amino acid selected from certain amino acids at specific positions important for conferring an increased HPPD inhibitor tolerance. The present invention also relates to proteins encoded by the nucleic acid of the invention, to chimeric genes, plant cells comprising the nucleic acid of the invention operably linked to a plant-expressible promoter and optionally a transcription termination and polyadenylation region, plants essentially consisting of the plant cells of the invention and methods of obtaining transgenic plants.Type: ApplicationFiled: March 7, 2013Publication date: September 11, 2014Applicant: BAYER CROPSCIENCE AGInventors: Gudrun Lange, Fabien Poree, Bernd Laber, Joerg Freigang, Arno Schultz
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IN VITRO AND IN VIVO DELIVERY OF GENES AND PROTEINS USING THE BACTERIOPHAGE T4 DNA PACKAGING MACHINE
Publication number: 20140256796Abstract: Described is T4 DNA packaging machine comprising: one or more DNA molecules packaged in a head of the T4 DNA packaging machine, one or more Hoc-fused proteins displayed on the head of the T4 DNA packaging machine, and one or more Soc-fused proteins displayed on the head of the T4 DNA packaging machine. Also described are methods of making and using such a T4 DNA packaging machine.Type: ApplicationFiled: December 4, 2013Publication date: September 11, 2014Applicant: THE CATHOLIC UNIVERSITY OF AMERICAInventor: Venigalla B. RAO -
Patent number: 8828699Abstract: Disclosed are: a eukaryotic amadoriase which is prepared by introducing a mutation into DNA encoding a eukaryotic amadoriase derived from a microorganism belonging to the genus Coniochaeta or Eupenicillium so as to introduce a substitution into a specific amino acid residue in the eukaryotic amadoriase, thereby overcoming the defect associated with thermal stability; a gene or recombinant DNA for the eukaryotic amadoriase; and a process for production of a eukaryotic amadoriase having excellent thermal stability.Type: GrantFiled: February 14, 2011Date of Patent: September 9, 2014Assignee: Kikkoman CorporationInventors: Kozo Hirokawa, Atsushi Ichiyanagi
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Patent number: 8828690Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding multizymes (i.e., single polypeptides having at least two independent and separable enzymatic activities) along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these multizymes in plants and oleaginous yeast are disclosed.Type: GrantFiled: April 3, 2008Date of Patent: September 9, 2014Assignee: E I du Pont de Nemours and CompanyInventors: Howard G. Damude, Anthony J. Kinney, Kevin G. Ripp, Quinn Qun Zhu
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Patent number: 8822170Abstract: Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.Type: GrantFiled: August 19, 2011Date of Patent: September 2, 2014Assignee: Promega CorporationInventors: Keith V. Wood, Monika G. Wood, Mary P. Hall
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Patent number: 8822662Abstract: The present invention relates to polynucleotides from Cochliobolus heterostrophus C5, Cyanothece sp. CCY0110, Mycocentrospora acerina and Hyaloperonospora parasitica, which code for desaturases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides according to the invention, and to the polypeptides encoded by the polynucleotides. The invention furthermore relates to antibodies against the polypeptides according to the invention. Finally, the invention also relates to production processes for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to their use as drugs, cosmetics, foodstuffs, feedstuffs, preferably fish food, or food supplements.Type: GrantFiled: December 8, 2009Date of Patent: September 2, 2014Assignee: BASF Plant Science Company GmbHInventors: Toralf Senger, Jörg Bauer, Johnathan A. Napier
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Patent number: 8822400Abstract: The present invention provides compositions and methods for producing a polyol oxidase in micoroorganisms, and the use of polyol oxidases in cleaning compositions. The invention includes cleaning compositions that contain combinations of two or more POx oxidases, and cleaning compositions that contain combinations of two or more POx oxidases and a perhydrolase. In particular, the invention provides methods for expressing polyol oxidases in bacterial hosts for use in detergent applications for cleaning, bleaching and disinfecting.Type: GrantFiled: January 18, 2013Date of Patent: September 2, 2014Assignee: Danisco US Inc.Inventors: Manoj Kumar, Susan M. Madrid, Hugh C. McDonald, Ayrookaran J. Poulose, Thomas Rand, Huaming Wang
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Publication number: 20140242574Abstract: There has been a demand for a codon-optimized gene for the mutated catalytic domain of Oplophorus luciferase, which is capable of efficiently expressing a protein both in a cultured animal cell and Escherichia coli. There has also been a demand for a substrate coelenterazine analogue showing a higher activity than that of native 19 kDa protein. The invention provides a polynucleotide comprising a polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 2. According to the invention, bis-coelenterazine is used as a substrate coelenterazine analogue suitable for the photoprotein encoded by the polynucleotide comprising the polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 2.Type: ApplicationFiled: February 25, 2014Publication date: August 28, 2014Applicant: JNC CORPORATIONInventors: Satoshi INOUYE, Junichi SATO
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Publication number: 20140242036Abstract: The instant invention refers to a novel process for potentiating the production of substances with antifungal activity obtained from Ganoderma lucidum, using a technological device for the differential, qualitative and quantitative expression of proteins and other bioactive molecules, selected from the group consisting of polysaccharides, triterpenoids, fatty acids and ganoderic acids. The compositions containing said substances showed antifungal activity and mycelium growth and fungi ascospore germination inhibiting activity, amongst them Mycosphaerella fijiensis, primary pathogenic agent causing black Sigatoka disease in banana and plantain crop fields.Type: ApplicationFiled: June 18, 2013Publication date: August 28, 2014Inventors: JOHN JAIRO MIRA CASTILLO, PAOLA ANDREA ZAPATA OCAMPO, LUCíA ATEHORTÚA GARCÉS, LIUDA JOHANA SEPÚLVEDA ARANGO, DIEGO FERNANDO ROJAS VAHOS
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Publication number: 20140238857Abstract: The present invention provides an arsenite oxidase enzyme modified to prevent translocation by modification of a translocation signal sequence. A microorganism modified to express the heterologous arsenite oxidase enzyme is also provided by the invention, together with a device for detecting the presence of arsenite in a sample.Type: ApplicationFiled: October 19, 2012Publication date: August 28, 2014Applicant: THE BIO NANO CENTRE LIMITEDInventors: Anthony Edward George Cass, Joanne Santini, Christopher James Johnson
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Publication number: 20140242647Abstract: The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention.Type: ApplicationFiled: February 20, 2014Publication date: August 28, 2014Applicant: California Institute of TechnologyInventors: Pedro S. Coelho, Eric M. Brustad, Frances H. Arnold, Zhan Wang, Jared C. Lewis
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Publication number: 20140234903Abstract: A novel gene cluster encoding polypeptides involved in the generation of bio synthetically unique peptide-based compounds is described. In addition, new methods for biosynthetic engineering and production of modified peptides and proteins are disclosed. Furthermore, new tools for identification of homologue polypeptides and precursor peptides in other species are provided. In addition, peptide-based compounds and fusions of these compounds with functional moieties for treatment of disorders such as tumors are disclosed.Type: ApplicationFiled: September 5, 2012Publication date: August 21, 2014Applicant: ETH ZURICHInventors: Jorn Piel, Cristian Gurgui, Michael Francis Freeman, Agustinus Robert Uria, Maximilian Johannes Helf
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Publication number: 20140234976Abstract: The present invention provides a protein, specifically Parietochloris incisa acetohydroxyacid synthase, and methods for producing branched-chain amino acid in a cell. The present invention further provides polypeptides and polynucleotides useful for conferring herbicide resistance in a cell or an organism. In particular, the present invention discloses a mutated acetohydroxyacid synthase which is resistant to herbicides.Type: ApplicationFiled: October 4, 2012Publication date: August 21, 2014Applicant: BEN-GURION UNIVERSITY OF THE NEGEV RESEARCH AND DEVELOPMENT AUTHORITYInventors: Omer Grundman, Inna Khozin-Goldberg, Zvi Hacohen, Michal Shapira, Dina Raveh, Sammy Boussiba, Boris Zorin
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Publication number: 20140227759Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.Type: ApplicationFiled: January 21, 2014Publication date: August 14, 2014Applicant: PROMEGA CORPORATIONInventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
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Publication number: 20140227729Abstract: The invention provides isolated nucleic acid encoding one or more gene products that allow for degradation of caffeine and related structures, and for preparation of intermediates in that catabolic pathway. Further provided are methods of using one or more of those gene products.Type: ApplicationFiled: October 18, 2012Publication date: August 14, 2014Applicant: University of Iowa Research Foundation Iowa Centers for EnterpriseInventors: Venkiteswaran Subramanian, Michael Tai-Man Louie, Ryan Summers
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Patent number: 8802401Abstract: The disclosure relates to engineered P450 polypeptides and use of such polypeptides in chemoenzymatic methods to construct selectively protected carbohydrates, which are useful as building blocks for preparation of carbohydrate derivatives and oligosaccharidesType: GrantFiled: June 18, 2008Date of Patent: August 12, 2014Assignees: The California Institute of Technology, The Scripps Research InstituteInventors: Frances H. Arnold, Chi-Huey Wong, Yuuichi Mitsuda, Michael M. Chen, Clay Bennett, William Greenberg, Jared Crawford Lewis, Sabine Bastian
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Patent number: 8802412Abstract: The present invention relates to isolated polynucleotides having promoter activity the use of the isolated polynucleotides for the production of a polypeptide. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing a desired polypeptide using the polypeptide having promoter activity.Type: GrantFiled: June 23, 2011Date of Patent: August 12, 2014Assignee: Novozymes A/SInventors: Hiroshi Teramoto, Hiroaki Udagawa, Rikako Taira
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Publication number: 20140223597Abstract: The present invention relates to a nucleic acid sequence encoding a mutated hydroxyphenylpyruvate dioxygenase (HPPD), to a chimeric gene which comprises this sequence as the coding sequence, and to its use for obtaining plants which are resistant to HPPD inhibitor herbicides.Type: ApplicationFiled: April 18, 2014Publication date: August 7, 2014Applicants: Bayer CropScience AG, Bayer BioScience N.V.Inventors: Marco Busch, Kerstin Fischer, Bernd Laber, Alain Sailland
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Publication number: 20140220631Abstract: Disclosed are a method for rapid screening of suitable translational fusion partners (TFPs) capable of inducing expression or secretory production of non-producible proteins, which are difficult to produce in conventional recombinant production methods, from a variety of genetic sources, and protein secretion-inducing TFPs obtained using the method.Type: ApplicationFiled: January 30, 2014Publication date: August 7, 2014Applicant: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGYInventors: Jung-Hoon SOHN, Eui-Sung CHOI, Jung-Hoon BAE, Eung-Suck LEE, Mi-Kyung SHIN
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Patent number: 8796002Abstract: The present disclosure relates to engineered ketoreductase polypeptides and uses thereof for the preparation of ? chloroalcohols from ? chloroketones. Also provided are polynucleotides encoding the engineered ketoreductase polypeptides and host cells capable of expressing the engineered ketoreductase polypeptides.Type: GrantFiled: June 22, 2010Date of Patent: August 5, 2014Assignee: Codexis, Inc.Inventors: Yong Koy Bong, Michael Vogel, Steven J. Collier, Vesna Mitchell, Jagadeesh Mavinahalli
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Patent number: 8790641Abstract: A device, system and method for producing glycosylated proteins in plant culture, particularly proteins having a high mannose glycosylation, while targeting such proteins with an ER signal and/or by-passing the Golgi. The invention further relates to vectors and methods for expression and production of enzymatically active high mannose lysosomal enzymes using transgenic plant root, particularly carrot cells. More particularly, the invention relates to host cells, particularly transgenic suspended carrot cells, vectors and methods for high yield expression and production of biologically active high mannose Glucocerebrosidase (GCD). The invention further provides for compositions and methods for the treatment of lysosomal storage diseases.Type: GrantFiled: April 23, 2009Date of Patent: July 29, 2014Assignee: Protalix Ltd.Inventors: Yoseph Shaaltiel, Gideon Baum, Daniel Bartfeld, Sharon Hashmueli, Ayala Lewkowicz
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Patent number: 8790901Abstract: Methods of producing an unsaturated free fatty acid comprising at least 18 carbon atoms are provided. In some embodiments, the methods comprise culturing an engineered microorganism in a culture medium, wherein the engineered microorganism comprises at least one recombinant enzyme selected from acyl-lipid desaturase delta-9 (EC:1.14.19.1), acyl-lipid desaturase delta-12 (EC:1.14.19.6), acyl-lipid desaturase delta-15 (EC:1.14.19.-), and thioesterase (EC:3.1.2.14). Engineered microorganisms comprising at least one of those recombinant enzymes are also provided. The methods and organisms can be used to produce at least one free fatty acid selected from oleic acid, linoleic acid and ?-linolenic acid.Type: GrantFiled: December 14, 2011Date of Patent: July 29, 2014Assignee: Pronutria, Inc.Inventors: Subhayu Basu, Gaozhong Shen, Brett Boghigian, David Young, David Berry, Geoffrey von Maltzahn
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Publication number: 20140206558Abstract: The present disclosure provides methods and compositions for the production of chimeric antibodies that specifically bind an antigen of interest.Type: ApplicationFiled: December 2, 2013Publication date: July 24, 2014Inventor: Alexey Gennadievich Zdanovsky
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Patent number: 8785147Abstract: A method for analyzing L-threonine contained in an specimen, which includes the steps of mixing a sample containing the specimen with an L-threonine dehydrogenase derived from Cupriavidus necator and a coenzyme NAD+ and analyzing the amount of NADH or 2-amino-3-oxobutyric acid after a predetermined period; an L-threonine dehydrogenase derived from Cupriavidus necator, which is a novel L-threonine dehydrogenase (TDH; EC 1.1.1.103) and can be utilized in the above-mentioned analysis method; a method for preparing a gene or the like to be used in the preparation of the enzyme, or a method for preparing the enzyme; an L-threonine analysis kit which includes (A) the L-threonine dehydrogenase and (B) a coenzyme NAD+; an enzyme preparation for use in the analysis of L-threonine, which includes the L-threonine dehydrogenase contained in a buffer solution; and an enzyme sensor utilizing the L-threonine dehydrogenase.Type: GrantFiled: September 4, 2012Date of Patent: July 22, 2014Assignees: Toyama Prefecture, Ajinomoto Co., Inc.Inventors: Yasuhisa Asano, Techawaree Ueatrongchit
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Patent number: 8785727Abstract: The present invention relates to a polynucleotide from Emiliana huxleyi which codes for a desaturase and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotide according to the invention, and to the polypeptides encoded by the polynucleotide. The invention furthermore relates to antibodies against the polypeptide according to the invention. Finally, the invention also relates to production methods for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to their use as drugs, cosmetics, foodstuffs, feedstuffs, preferably fish food, or food supplements.Type: GrantFiled: April 29, 2009Date of Patent: July 22, 2014Assignee: Rothamsted Research Ltd.Inventors: Joerg Bauer, Johnathan A. Napier, Olga Sayanova
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Patent number: 8785163Abstract: The present invention relates to polynucleotides from Helobdella robusta, Laccaria bicolor, Lottia gigantea, Microcoleus chthonoplastes, Monosiga brevicollis, Mycosphaerella fijiensis, Mycospaerella graminicola, Naegleria gruberi, Nectria haematococca, Nematostella vectensis, Phycomyces blakesleeanus, Trichoderma resii, Physcomitrella patens, Postia placenta, Selaginella moellendorffii and Microdochium nivale, which code for desaturases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides according to the invention, and to the polypeptides encoded by the polynucleotides. The invention furthermore relates to antibodies against the polypeptides according to the invention.Type: GrantFiled: July 30, 2008Date of Patent: July 22, 2014Assignee: BASF Plant Science GmbHInventors: Toralf Senger, Jörg Bauer
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Publication number: 20140201855Abstract: Described are mutant luciferases, nucleic acids that encode them, cells and animals expressing them, methods of use thereof, and kits.Type: ApplicationFiled: February 9, 2012Publication date: July 17, 2014Applicant: UNIVERSITY OF MASSACHUSETTSInventors: Stephen C. Miller, David Mofford, Katryn Harwood
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Publication number: 20140199735Abstract: The present disclosure relates to biocatalysts and its uses for the efficient preparation of eslicarbazepine, eslicarbazepine acetate, and analogs thereof.Type: ApplicationFiled: April 12, 2012Publication date: July 17, 2014Applicant: CODEXIS, INC.Inventors: Anupam Gohel, Derek Smith, Brian Wong, Joly Sukumaran, Wan Lin Yeo, Steven J. Collier, Scott Novick
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Patent number: 8778639Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: February 12, 2013Date of Patent: July 15, 2014Assignee: Novozymes Inc.Inventor: Nikolaj Spodsberg
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Patent number: 8778658Abstract: The technology relates in part to biological methods for producing adipic acid and engineered microorganisms capable of such production.Type: GrantFiled: July 13, 2012Date of Patent: July 15, 2014Assignee: Verdezyne, Inc.Inventors: Stephen Picataggio, Tom Beardslee
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Patent number: 8778641Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: February 12, 2013Date of Patent: July 15, 2014Assignee: Novozymes Inc.Inventor: Nikolaj Spodsberg
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Patent number: 8778640Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: February 12, 2013Date of Patent: July 15, 2014Assignee: Novozymes Inc.Inventor: Nikolaj Spodsberg
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Publication number: 20140193869Abstract: The present disclosure relates, in some aspects, to cell-free methods and systems for large-scale conversion of methane to isobutanol, comprising combining, in a bioreactor at elevated pressure, methane, oxygen, and cell lysates containing methane monooxygenase, methanol dehydrogenase, and enzymes that catalyze the conversion of formaldehyde to isobutanol, to form a cell-free reaction mixture, and incubating under suitable conditions the cell-free reaction to convert methane to isobutanol.Type: ApplicationFiled: December 20, 2013Publication date: July 10, 2014Applicant: GreenLight Biosciences, Inc.Inventors: William Jeremy Blake, James R. Swartz
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Patent number: 8771993Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: February 12, 2013Date of Patent: July 8, 2014Assignees: Novozymes A/S, Novozymes Inc.Inventor: Nikolaj Spodsberg
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Patent number: 8771994Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: February 12, 2013Date of Patent: July 8, 2014Assignee: Novozymes Inc.Inventor: Nikolaj Spodsberg
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Patent number: 8772005Abstract: Novel laccases, nucleic acid sequences encoding such laccases, and vectors and host cells for expressing the laccases are described. The novel laccase enzymes may be employed in conjunction with mediators to provide an improved method for bleaching denim fabrics.Type: GrantFiled: December 6, 2011Date of Patent: July 8, 2014Assignee: Danisco US Inc.Inventors: Huaming Wang, Joseph C. McAuliffe
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Publication number: 20140186918Abstract: A recombinant protein having luciferase activity and at least 60% similarity to a wild-type luciferase wherein in the sequence of the enzyme, the amino acid residue corresponding to residue 357 in Photinus pyralis luciferase is mutated as compared to the corresponding wild-type luciferase, such that the luciferase enzyme is able to emit light at a different wavelength as compared to the corresponding wild-type luciferase and/or has enhanced thermostability as compared to the corresponding wild-type luciferase. In general, the residue corresponding to 357 in Photinus pyralis luciferase is changed from an acidic amino acid to a non-acidic amino acid, and preferably an uncharged polar amino acid such as tyrosine. Mutant luciferases in accordance with the invention can produce a large (50 nm) wavelength shift in emitted light and have good thermostability. The resultant colour shift can be reversed by addition of coenzyme A. These properties make the mutant particularly useful in a variety of assays.Type: ApplicationFiled: February 14, 2014Publication date: July 3, 2014Applicant: PROMEGA CORPORATIONInventors: David James Squirrell, Melenie Jane Murphy, Rachel Louise Price, Peter John White, Tara Louise Willey
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Publication number: 20140186324Abstract: This invention provides methods of isolating ferritin from plant and animal material. The isolated ferritin can be administered to humans or animals in need of iron, and can be used to treat or supplement iron deficiency. The isolated ferritin can be used in industrial applications, such as increasing the iron content in heat-processed food or beverages. The methods of the invention also include quantitation of iron derived from plant or animal ferritin.Type: ApplicationFiled: March 4, 2014Publication date: July 3, 2014Applicant: Slo-Iron, LLCInventor: Elizabeth C. Theil
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Publication number: 20140186429Abstract: The present invention provides a method of biosynthesizing a recombinant protein containing the biologically active peptide, the pharmaceutical composition containing the recombinant protein as well as the preparation of the recombinant protein. Simply speaking the consecutively multiple copies of the bioactive peptide are replaced in the amino acid sequence of a recombinant protein (so called peptide-protein). Then, a high concentration and high yield of the peptide-protein containing the consecutively multiple copies of the bioactive peptide is produced by the biosafety and edible strain of yeast without any endotoxin contamination.Type: ApplicationFiled: September 11, 2013Publication date: July 3, 2014Inventor: Hung-Chien CHIEN
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Patent number: 8765446Abstract: The present invention relates to recombinant mutant microorganisms having an increased ability to produce alcohol and a method of producing alcohol using the same, and more particularly to recombinant mutant microorganisms which have an increased ability to produce butanol, ethanol, isopropanol or mixed alcohols, which can be used as fuel, while producing little or no producing acetone as a byproduct, and to a method of producing butanol, ethanol, isopropanol or mixed alcohols using the same. The inventive recombinant mutant microorganisms having an increased ability to produce butanol or mixed alcohols and to remove acetone are those in which genes that encode enzymes involved in producing butanol from butyryl-CoA or butylaldehyde and in producing isopropanol from acetone were amplified or introduced in host microorganisms.Type: GrantFiled: September 21, 2010Date of Patent: July 1, 2014Assignees: Korea Advanced institute of Science and Technology, Biofuelchem Co., GS Caltex CorporationInventors: Sang Yup Lee, Yu-Sin Jang, Joung Min Lee, Jung Ae Im, Hyohak Song
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Publication number: 20140179942Abstract: The invention relates to a micro-organism comprising a hydrogenase enzyme system which is capable of converting carbon dioxide into formic acid and a second enzyme system which is capable of converting formic acid into aliphatic carboxylic acids having a chain length of five or more carbon atoms. Also described are various methods for producing oil, as well as other aspects of the invention.Type: ApplicationFiled: July 13, 2012Publication date: June 26, 2014Applicant: VERDANT BIOPRODUCTS LIMITEDInventor: Irene Finnegan
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Publication number: 20140178933Abstract: An expression vector which is capable of overexpressing a protein of interest in a host cell, a host cell comprising the expression vector, and a method of producing a protein of interest are provided.Type: ApplicationFiled: April 30, 2012Publication date: June 26, 2014Applicants: RESEARCH & BUSINESS FOUNDATION SUNGKYUNKWAN UNIVERSITY, Samsung Electronics Co., Ltd.Inventors: Hyun Min Koo, Byung Jo Yu, Ki Sung Lee, Jae Chan Park, Dae Hyuk Kweon, Sung Minm Park
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Patent number: 8759058Abstract: The present invention relates generally to hydrogen production for use in fuel cells, foodstuffs and chemical production, and more particularly, to biologically and photosynthetically produced hydrogen. Specifically, disclosed is a method for producing bacteria and green alga that can produce hydrogen in quantities that exceed four hundred percent of the hydrogen produced by green alga in nature; thus, producing organisms which can serve as hydrogen generators for fuel cells, chemical production and numerous other applications.Type: GrantFiled: February 24, 2012Date of Patent: June 24, 2014Inventors: Scott Plummer, Mark Plummer
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Patent number: 8759059Abstract: Methods and materials related to producing 3-HP as well as other organic compounds are disclosed. Specifically, isolated nucleic acids, polypeptides, host cells, and methods and materials for producing 3-HP and other organic compounds are disclosed.Type: GrantFiled: June 25, 2013Date of Patent: June 24, 2014Assignee: Cargill, IncorporatedInventors: Ravi R. Gokarn, Olga V. Selifonova, Holly Jean Jessen, Steven John Gort, Thorsten Selmer, Wolfgang Buckel
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Patent number: 8759040Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: February 12, 2013Date of Patent: June 24, 2014Assignee: Novozymes Inc.Inventor: Nikolaj Spodsberg
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Patent number: 8759060Abstract: The present invention is directed to an arginine/lysine oxidoreductase modified with polyethylene glycol, a production method thereof, and methods of treating disorders responsive to a modification of amino acid levels reactive oxygen species and/or ammonium.Type: GrantFiled: September 9, 2013Date of Patent: June 24, 2014Inventors: Christian J. Hofmann, Michael Lindemann
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Patent number: 8759041Abstract: The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: February 12, 2013Date of Patent: June 24, 2014Assignee: Novozymes Inc.Inventor: Nikolaj Spodsberg
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Patent number: 8759057Abstract: A process for purifying laccase from an ectomycorrhizal fruiting body is disclosed. The process includes steps of homogenization, sonication, centrifugation, filtration, affinity chromatography, ion exchange chromatography, and gel filtration. Purified laccase can also be separated into isomers.Type: GrantFiled: April 1, 2013Date of Patent: June 24, 2014Assignee: The United States of America as Represented by the Administrator of the National Aeronautics & Space Administration (NASA)Inventors: Kenneth W. Cullings, Julia C. DeSimone, Chad D. Paavola