Transferase Other Than Ribonuclease (2.) Patents (Class 435/193)
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Patent number: 8183005Abstract: The present inventors examined the inhibitory activity against HCV replicon of myriocin, fumonisin B1, and ceramide trafficking inhibitor HPA-12, which are compounds derived from microorganisms such as those of the genus Aureobasidium, and found that these compounds have the effect of inhibiting the replication of HCV replicon RNAs or inhibiting the expression of HCV proteins. The present inventors further performed serine palmitoyltransferase knockdown experiments using siRNAs, and results showed that HCV replicon activity and HCV protein expression were significantly inhibited in cells with suppressed LCB1 expression. Thus, sphingolipid biosynthesis was found to be involved in HCV infection. This showed that HCV infection can be treated or prevented by inhibiting enzyme activities involved in the process of sphingolipid biosynthesis by adding compounds or knocking down a gene.Type: GrantFiled: August 11, 2005Date of Patent: May 22, 2012Assignee: Chugai Seiyaku Kabushiki KaishaInventors: Masayuki Sudo, Hiroshi Sakamoto
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Publication number: 20120124705Abstract: The invention provides isolated nucleic acid molecules which encode novel fatty acid desaturases and elongases from the organism Emiliana huxleyi. The invention also provides recombinant expression vectors containing desaturase or elongase nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g. arachidonic acid (ARA), eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA).Type: ApplicationFiled: July 15, 2010Publication date: May 17, 2012Applicant: BASF Plant Science Company GMBHInventors: Jörg Bauer, Johnathan A. Napier, Olga Sayanova
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Publication number: 20120115186Abstract: The present invention relates to a process for the production of one or more fermentation product from a sugar composition, comprising the following steps: a) fermentation of the sugar composition in the presence of a yeast belonging to the genera Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Kloeckera, Schwanniomyces or Yarrowia, and b) recovery of the fermentation product, wherein the yeast comprises the genes araA, araB and araD and the sugar composition comprises glucose, galactose and arabinose.Type: ApplicationFiled: July 6, 2010Publication date: May 10, 2012Applicant: DSM IP ASSETS B.V.Inventors: Paul Klaassen, Gijsberdina Pieternella Van Suylekom, Bianca Elisabeth Maria Gielesen, Nicolette Jasmijn Broers, Beate Wiedemann, Wilhelmus Theodorus Antonius Maria De Laat
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Publication number: 20120115153Abstract: The present invention relates to a marker for the prognosis of liver cancer; a composition for estimating the prognosis of liver cancer, which contains a substance for detecting a change in the expression level of the prognostic marker for liver cancer; a kit for estimating the prognosis of liver cancer, which contains the composition for estimating liver cancer prognosis; a method for estimating the prognosis of liver cancer using the marker for liver cancer prognosis; and a method for screening a therapeutic agent for liver cancer using the marker for the prognosis of liver cancer.Type: ApplicationFiled: April 16, 2010Publication date: May 10, 2012Applicants: CBS BIOSCIENCE, CO., LTD.Inventors: Jin young Park, Seok joo Hong, Jong Min Kim
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Publication number: 20120117694Abstract: Modified (iso)eugenol 4-O-methyltransferase enzymes having novel capacity for methylation of monolignols and reduction of lignin polymerization in plant cell wall are disclosed. Sequences encoding the modified enzymes are disclosed.Type: ApplicationFiled: October 28, 2011Publication date: May 10, 2012Applicant: Brookhaven Science Associates, LLCInventors: Chang-Jun Liu, Mohammad-Wadud Bhuiya, Kewei Zhang
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Publication number: 20120115231Abstract: The present invention provides novel lysophospholipid acyltransferases. The object of the present invention is attained by the nucleotide sequences of SEQ ID NOs: 1 and 6 and the amino acid sequences of SEQ ID NOs: 2 and 7 of the present invention.Type: ApplicationFiled: March 25, 2010Publication date: May 10, 2012Inventor: Misa Ochiai
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Publication number: 20120114688Abstract: Compositions and methods for preventing, treating and detecting leishmaniasis are disclosed. The compositions generally comprise fusion polypeptides comprising Leishmania antigens, in particular, SMT and NH antigens or immunogenic portions or variants thereof, as well as polynucleotides encoding such fusion polypeptides.Type: ApplicationFiled: November 7, 2011Publication date: May 10, 2012Applicant: INFECTIOUS DISEASE RESEARCH INSTITUTEInventors: Ajay Bhatia, Steven G. Reed
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Publication number: 20120115188Abstract: The present invention provides, among other things, chimeric DNA polymerases containing heterologous domains having sequences derived from at least two DNA polymerases that have at least one distinct functional characteristics (e.g., elongation rate, processivity, error rate or fidelity, salt tolerance or resistance) and methods of making and using the same. In some embodiments, the present invention can combine desired functional characteristics (e.g., high processivity; high elongation rate; thermostability; resistance to salt, PCR additives (e.g., PCR enhancers) and other impurities; and high fidelity) of different DNA polymerases in a chimeric polymerase.Type: ApplicationFiled: November 3, 2009Publication date: May 10, 2012Applicant: KAPA BIOSYSTEMSInventors: Bjarne Faurholm, Paul McEwan, William Bourn, Gavin Rush
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Publication number: 20120116058Abstract: A novel gene encoding P. pastoris orotate-phosphoribosyl transferase (URA5) is disclosed. Methods for producing and selecting yeast strains capable of stable genetic integration of heterologous sequences into the host genome are also provided.Type: ApplicationFiled: October 7, 2011Publication date: May 10, 2012Applicant: Glycofi, Inc.Inventor: Juergen H. Nett
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Patent number: 8173103Abstract: P-Selectin on platelets and endothelium binds cell surface chondroitin sulfate (CS) proteoglycans, which are abundantly and stably expressed on the surface many cancer cells. Binding of the cancer cells through the CS moieties may be blocked to inhibit the interaction of cancer cells with platelets and endothelium. The present inventors disclose compositions and methods for the inhibition of cancer metastasis.Type: GrantFiled: October 4, 2008Date of Patent: May 8, 2012Assignee: The Board of Trustees of the University of ArkansaInventors: Behjatolah M. Karbassi, Thomas Kieber-Emmons
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Patent number: 8173399Abstract: A method for producing lacto-N-biose I and galacto-N-biose inexpensively and conveniently is provided. The method for producing lacto-N-biose I or galacto-N-biose, characterized in that the method comprises causing: (i) a combination of a carbohydrate raw material with an enzyme that catalyzes phosphorolysis of the carbohydrate raw material to give ?-glucose-1-phosphate; and (ii) a combination of an enzyme that converts ?-glucose-1-phosphate to UDP-glucose and an enzyme that converts UDP-galactose to galactose-1-phosphate with their cofactors, and/or a combination of an enzyme (UDP-Gly synthase) that converts ?-glucose-1-phosphate and UDP-galactose to UDP-glucose and ?-galactose-1-phosphate, respectively, with its cofactor(s) to act in the presence of N-acetylglucosamine or N-acetylgalactosamine, phosphoric acid, lacto-N-biose phosphorylase (EC 2.4.1.211), and UDP-glucose-4-epimerase (EC 5.1.3.2).Type: GrantFiled: December 12, 2007Date of Patent: May 8, 2012Assignee: Incorporated Administrative Agency National Agriculture and Food Research OrganizationInventors: Motomitsu Kitaoka, Mamoru Nishimoto
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Patent number: 8173364Abstract: This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo.Type: GrantFiled: October 26, 2007Date of Patent: May 8, 2012Assignees: The Scripps Research Institute, The Regents of the University of CaliforniaInventors: Peter G. Schultz, Lei Wang, John Christopher Anderson, Jason W. Chin, David R. Liu, Thomas J. Magliery, Eric L. Meggers, Ryan Aaron Mehl, Miro Pastrnak, Stephen William Santoro, Zhiwen Zhang
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Publication number: 20120110703Abstract: This invention provides DNAs of a prenyltransferase derived from a hop (Humulus) plant. The invention also provides protein having the prenyltransferase activity of a hop plant and a method for producing and detecting a novel organism using a gene encoding such protein.Type: ApplicationFiled: June 18, 2010Publication date: May 3, 2012Applicant: KIRIN HOLDINGS KABUSHIKI KAISHAInventors: Kazufumi Yazaki, Naoyuki Umemoto, Masaki Momose
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Publication number: 20120108449Abstract: A crystal comprising human isoglutaminyl cyclase having a characterised space group of P1211 and unit cell dimensions of +/?5% of a=126.51 ?, b=109.68 ?, c=159.53 ?, ?=90.0°, ?=104.9° and ?=90.0°.Type: ApplicationFiled: October 28, 2011Publication date: May 3, 2012Applicant: PROBIODRUG AGInventors: Anett Stephan, Stephan Schilling, Jens-Ulrich Rahfeld, Michael Wermann, Christoph Parthier, Hans-Ulrich Demuth, David Ruiz-Carillo, Milton T. Stubbs
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Patent number: 8168417Abstract: The present invention relates to an isolated polynucleotide of the complete chromosome of Bacillus licheniformis. The present invention also relates to isolated genes of the chromosome of Bacillus licheniformis which encode biologically active substances and to nucleic acid constructs, vectors, and host cells comprising the genes as well as methods for producing biologically active substances encoded by the genes and to methods of using the isolated genes of the complete chromosome of Bacillus licheniformis.Type: GrantFiled: December 17, 2010Date of Patent: May 1, 2012Assignees: Novozymes A/S, Novozymes, Inc.Inventors: Randy Berka, Michael Rey, Preethi Ramaiya, Jens Tønne Andersen, Michael Dolberg Rasmussen, Peter Bjarke Olsen
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Patent number: 8168416Abstract: The invention provides compounds, pharmaceutical compositions and methods for treating atherosclerosis, coronary heart disease, thrombosis, and for decreasing or prevention of accumulation of cholesterol in a subject by modifying LCAT polypeptide.Type: GrantFiled: July 25, 2008Date of Patent: May 1, 2012Assignee: Amgen Inc.Inventors: Tom Boone, David Park Meininger, Margrit Schwarz, Bei Shan, Wenyan Shen, Mingyue Zhou
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Publication number: 20120100594Abstract: The present invention provides enzyme catalysts for Diels-Alder reactions, including intermolecular Diels-Alder reactions, as well as protein scaffolds for making such enzyme catalysts. In other aspects, the invention provides methods of making the enzyme catalysts, including by de novo computational design. The present invention thereby provides enzyme catalysts capable of catalyzing a desired Diels-Alder reaction, including with a specified or desired stereo-selectivity.Type: ApplicationFiled: November 19, 2009Publication date: April 26, 2012Applicant: University of WashingtonInventors: David Baker, Alexandre Zangellini, Justin Siegel, Jennifer St. Clair
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Publication number: 20120100595Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) and using these delta-9 elongases in plants.Type: ApplicationFiled: January 11, 2012Publication date: April 26, 2012Inventors: Howard Glenn DAMUDE, Quinn Qun Zhu
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Publication number: 20120100253Abstract: The object is to provide a novel glycosyltransferase and the use thereof, the glycosyltransferase catalyzes transglucosylation of maltotriose units under conditions which can be employed for the processing of foods or the like. Provided is a maltotriosyl transferase which acts on polysaccharides and oligosaccharides having ?-1,4 glucoside bonds, and has activity for transferring maltotriose units to saccharides, the maltotriosyl transferase acting on maltotetraose as substrate to give a ratio between the maltoheptaose production rate and maltotriose production rate of 9:1 to 10:0 at any substrate concentration ranging from 0.67 to 70% (w/v).Type: ApplicationFiled: March 20, 2010Publication date: April 26, 2012Applicant: AMANO ENZYME INC.Inventors: Masamichi Okada, Shotaro Yama, Miho Nagaya
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Publication number: 20120102596Abstract: Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods for their use are provided. The compositions are novel molecular marker loci genetically linked with QTL6 and which are associated with increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio of a plant. These novel markers are characterized by the presence of at least one polymorphism relative to the corresponding marker locus from the QTL6 region of non-high-oil, non-high-oleic acid maize plants. In some embodiments, the novel marker loci comprise coding sequence for a maize DGAT1-2 polypeptide or biologically active variant thereof. The marker loci of the invention, and suitable fragments thereof, are useful in methods for manipulating oil and/or oleic acid content and/or oleic acid/linoleic acid ratio of a plant, for marker-assisted selection of a plant, and for marker-assisted breeding of the high oil and/or high oleic acid trait.Type: ApplicationFiled: November 18, 2011Publication date: April 26, 2012Applicants: E.I. du Pont de Nemours and Company, Pioneer Hi-Bred International, Inc.Inventors: William B. Allen, Bo Shen, Mitchell C. Tarczynski, Mark E. Williams, Peizhong Zheng, Gan-Yuan Zhong
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Publication number: 20120094358Abstract: Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids.Type: ApplicationFiled: December 6, 2011Publication date: April 19, 2012Applicant: XYLECO, INC.Inventor: Marshall Medoff
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Publication number: 20120096588Abstract: Transgenic soybean seed having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of non-transgenic, null segregant soybean seed are described. DGAT genes from oleaginous organisms are used to achieve the increase in seed storage lipids.Type: ApplicationFiled: December 16, 2011Publication date: April 19, 2012Applicant: E.I. du Pont de Nemours and CompanyInventors: Knut Meyer, Howard Glenn Damude, Kevin G. Ripp, Kevin L. Stecca
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Publication number: 20120094280Abstract: The present invention relates to a use of non-cofactor compounds, represented by formulas (I) or (II) wherein R and Z are independently selected from H, D, C1-C12-alkyl, preferably C1-C4-alkyl, alkenyl, alkinyl, phenyl or -LX, wherein X represents a functional group or a reporter group attached via a linker group L, and QH is selected from —SH, —SeH, —NHNH2 or —ONH2, for a targeted modification or derivatization of a biomolecule by covalent coupling to the biomolecule in the presence of a directing methyltransferase. Further development of the method of targeted modification and derivatization are the method for targeted labeling a biomolecule and method for detecting unmethylated target sites in a biomolecule comprising modification of the biomolecule according to the present invention.Type: ApplicationFiled: April 1, 2010Publication date: April 19, 2012Applicant: BIOTECHNOLOGIJOS INSTITUTASInventors: Saulius Klimasauskas, Zita Liutkeviciute, Edita Kriukiene
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Publication number: 20120094862Abstract: The invention provides histone deacetylase class II nucleic acids and polypeptides, methods and reagents for their use, and related compounds including small molecule libraries containing class II histone deacetylase inhibitors.Type: ApplicationFiled: December 13, 2011Publication date: April 19, 2012Applicant: President and Fellows of Harvard CollegeInventors: Christina M. Grozinger, Christian A. Hassig, Stuart L. Schreiber
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Publication number: 20120096592Abstract: The present invention relates generally to the field of molecular biology and concerns a method for enhancing yield related traits by modulating expression in a plant of a nucleic acid encoding a BET1-like polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding this BET1-like polypeptide, which plants have enhanced yield-related traits relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention. The present invention relates generally to the field of molecular biology and concerns a method for improving various plant growth characteristics by modulating expression in a plant of a nucleic acid encoding a CRT (Calreticulin). The present invention also concerns plants having modulated expression of a nucleic acid encoding a Calreticulin, which plants have improved growth characteristics relative to corresponding wild type plants or other control plants.Type: ApplicationFiled: February 19, 2010Publication date: April 19, 2012Applicant: BASF Plant Science Company GmbHInventors: Yves Hatzfeld, Ana Isabel Sanz Molinero, Valerie Frankard, Christophe Reuzeau
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Publication number: 20120093850Abstract: Staphylococcus aureus Hla polypeptides having various deletions, insertions, and/or mutations which are useful for immunisation are provided herein. Also provided herein are Hla heptamers which are non-haemolytic. Additionally, an effective Staphylococcus aureus vaccine may require several antigenic components, and so various combinations of S. aureus antigens, including Hla polypeptides having deletions, insertions, and/or mutations, are identified for use in immunisation. These polypeptides may optionally be used in combination with S. aureus saccharides.Type: ApplicationFiled: September 15, 2011Publication date: April 19, 2012Applicant: NOVARTIS AGInventors: Fabio BAGNOLI, Massimiliano Biagini, Luigi Fiaschi, Guido Grandi, Ravi Pratap Narayan Mishra, Nathalie Norais, Maria Scarselli
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Patent number: 8158389Abstract: Products and methods for the in vivo production of monatin sweetener are provided. The products include microorganisms that are genetically modified to secrete or to improve secretion of monatin; microorganisms that are genetically modified to produce monatin; and microorganisms that are genetically modified to both secrete or improve secretion of monatin and produce monatin. The methods include producing monatin in such genetically engineered microorganisms.Type: GrantFiled: October 10, 2006Date of Patent: April 17, 2012Assignee: Cargill, IncorporatedInventors: James C. Anderson, Mervyn L. DeSouza, Paula M. Hicks, Sherry R. Kollmann
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Patent number: 8158400Abstract: The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: GrantFiled: November 10, 2011Date of Patent: April 17, 2012Assignees: Novozymes, Inc., Novozymes A/SInventors: Debbie Yaver, Suzanne Otani, Janine Lin, Christopher Amolo, Kim Borch, Shamkant Anant Patkar, Michael Lamsa, Barbara Cherry
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Patent number: 8158390Abstract: The invention relates to a method for producing L-valine and to a suitable microorganism. The inventive method is characterized by preferably enhancing the transaminase C activity of a coryneform bacterium, especially Corynebacterium glutamicum. The organisms so modified have a yield in L-valine which is 35.8% higher than that of non-modified organisms.Type: GrantFiled: August 19, 2005Date of Patent: April 17, 2012Assignee: Forschungszentrum Julich GmbHInventors: Jan Marienhagen, Lothar Eggeling, Hermann Sahm
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Publication number: 20120088281Abstract: The present invention relates to a modified thiolase protein with an improved activity, a polynucleotide encoding the modified protein, an expression vector including the polynucleotide, and a transformant, to a composition for producing a biobutanol including the thiolase with an improved activity or a cell expressing the thiolase, and to a method of producing the biobutanol.Type: ApplicationFiled: October 5, 2011Publication date: April 12, 2012Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATIONInventor: Kyung Jin KIM
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Patent number: 8153405Abstract: Products and methods for the in vivo production of monatin sweetener are provided. The products include microorganisms that are genetically modified to secrete or to improve secretion of monatin; microorganisms that are genetically modified to produce monatin; and microorganisms that are genetically modified to both secrete or improve secretion of monatin and produce monatin. The methods include producing monatin in such genetically engineered microorganisms.Type: GrantFiled: April 20, 2006Date of Patent: April 10, 2012Assignee: Cargill, IncorporatedInventors: James C. Anderson, Mervyn L. DeSouza, Paula M. Hicks, Sherry R. Kollmann, Jose Laplaza
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Patent number: 8153364Abstract: An emulsion is useful in allowing a wide variety of gene products to be expressed via eukaryotic in vitro expression. The emulsion comprises a silicone based surfactant, a hydrophobic phase and a hydrophilic phase; wherein the hydrophilic phase comprises a plurality of compartments containing a functional in vitro eukaryotic expression system.Type: GrantFiled: January 26, 2010Date of Patent: April 10, 2012Assignee: MRCInventors: Philipp Holliger, Farid Ghadessy
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Patent number: 8153410Abstract: A composition having an agent adapted to affect a multimeric protein by binding to a binding site of the multimeric protein and thereby affecting an equilibrium of units, wherein the multimeric protein has an assembly having a plurality of said units, wherein each of the units has a first complementary surface and a second complementary surface and wherein the first complementary surface of one unit is associated with the second complementary surface of another unit, provided that the assembly is at least one of different quaternary isoforms on a condition that in the multimeric protein (1) a structure of each of the units determines a structure of the different quaternary isoforms, (2) the units are in the equilibrium and (3) the structure of the different quaternary isoforms influences a function of the multimeric protein.Type: GrantFiled: June 19, 2008Date of Patent: April 10, 2012Assignee: Fox Chase Cancer CenterInventor: Eileen K. Jaffe
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Publication number: 20120084871Abstract: The present invention provides, in part, NPC1L1 from various species. Methods of using the NPC1L1 polypeptides and polynucleotide set forth herein, e.g., in screening assays, are also set forth.Type: ApplicationFiled: February 14, 2011Publication date: April 5, 2012Applicant: Schering CorporationInventors: Scott Altmann, Xiaorui Yao, Kim Anne O'Neill, Brian E. Hawes
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Publication number: 20120083036Abstract: The present invention is predicated on the discovery that a polysaccharide transferase purified from barley seedlings can catalyze the formation of covalent bonds between different polysaccharides, including between xyloglucans and cellulose, and between xyloglucans and (1,3;1,4)-?-D-glucans. The present invention thus provides, among other things, an isolated or substantially purified polysaccharide transferase, wherein said polysaccharide transferase is capable of catalyzing the formation of a covalent bond between a donor polysaccharide and an acceptor polysaccharide; or a functionally active fragment or variant of said polysaccharide transferase.Type: ApplicationFiled: December 14, 2011Publication date: April 5, 2012Applicant: ADELAIDE RESEARCH AND INNOVATION PTY LTDInventors: Maria Hrmova, Geoffrey Bruce Fincher
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Publication number: 20120083013Abstract: The invention provides a polynucleotide encoding a furofuran lignan 4-O-glucosyltransferase, and others. The polynucleotide in accordance with the present invention comprises a polynucleotide consisting of the nucleotide sequence represented by SEQ ID NO: 3 or SEQ ID NO: 5, or a polynucleotide encoding a protein consisting of the amino acid sequence represented by SEQ ID NO: 4 or SEQ ID NO: 6.Type: ApplicationFiled: May 18, 2010Publication date: April 5, 2012Applicant: Suntory Holdings LimitedInventors: Eiichiro Ono, Honoo Satake, Hyun-Jung Kim
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Patent number: 8148124Abstract: The invention relates to the cDNA and deduced amino acid sequence of the Coactivator Associated arginine (R) Methyltransferase protein, CARM1. A method is described for the use CARM1 to regulate gene expression in vivo. CARM1 has also been used to methylate arginine residues of histones, synthetic peptides, and other proteins. A method to use CARM1 to screen for drugs that inhibit its methyltransferase activity is also described, as is a method to screen for drugs that modulate CARM1's interactions with other proteins.Type: GrantFiled: April 17, 2007Date of Patent: April 3, 2012Assignees: The Regents of the University of California, University of Southern CaliforniaInventors: Michael R. Stallcup, Dagang Chen, Heng Hong, Dana W. Aswad
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Patent number: 8148125Abstract: Compositions comprising truncated tryptophanyl-tRNA synthetase polypeptides useful for regulating angiogenesis, as well as nucleic acids encoding such tRNA synthetase polypeptides are described. Methods of making and using such compositions are also disclosed.Type: GrantFiled: January 17, 2011Date of Patent: April 3, 2012Assignee: The Scripps Research InstituteInventors: Paul Schimmel, Keisuke Wakasugi
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Patent number: 8143031Abstract: The present invention claims an isolated polypeptide having L-amino-acid-N-acyl transferase enzymatic activity and a modified microorganism in which this enzyme is overexpressed. Substrates of said enzyme include mainly methionine and their derivatives or analogs. Overexpression in sulphur-containing amino acid producing microorganisms permits the production of large amounts of N-acylated sulphur-containing amino acids. The isolation of the N-acylated sulphur-containing amino acids from the fermentation medium is also claimed.Type: GrantFiled: February 12, 2009Date of Patent: March 27, 2012Assignee: Metabolic ExplorerInventors: Rainer Figge, Guillaume Barbier, Gwénaëlle Bestel-Corre
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Patent number: 8143036Abstract: Genetically modified microorganisms that produce itaconic acid at high yields and uses thereof.Type: GrantFiled: May 11, 2009Date of Patent: March 27, 2012Assignee: Industrial Technology Research InstituteInventors: James C. Liao, Pei-Ching Chang
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Publication number: 20120070863Abstract: The present invention provides a novel ?-galactoside-?2,3-sialyltransferase and a nucleic acid encoding the sialyltransferase. The present invention also provides a microorganism producing the sialyltransferase, as well as a method for producing the sialyltransferase using such a microorganism. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant ?-galactoside-?2,3-sialyltransferase. The present invention further provides a method for preparing a sialylsugar chain, which uses the sialyltransferase of the present invention.Type: ApplicationFiled: August 25, 2011Publication date: March 22, 2012Inventors: Takeshi YAMAMOTO, Hiroshi TSUKAMOTO, Yoshimitsu TAKAKURA
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Patent number: 8137928Abstract: The present invention provides enhanced methods of producing soluble, active eukaryotic glycosyltransferases in prokaryotic microorganisms that have an oxidizing environment.Type: GrantFiled: March 24, 2006Date of Patent: March 20, 2012Assignee: BioGeneriXInventors: Marc F. Schwartz, Tarik Soliman
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Patent number: 8137941Abstract: A chondroitin polymerase having such properties that it transfers GlcUA and GalNAc alternately to a non-reduced terminal of a sugar chain from a GlcUA donor and a GalNAc donor, respectively, and the like; and a process for producing the chondroitin polymerase.Type: GrantFiled: August 10, 2009Date of Patent: March 20, 2012Assignee: Seikagaku CorporationInventors: Toshio Ninomiya, Nobuo Sugiura, Koji Kimata
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Patent number: 8138321Abstract: A nucleic acid encoding an acetolactate synthase (ALS) protein that provides resistance to ALS inhibitors, e.g., sulphonylurea and imidazolinone compounds, is provided. The nucleic acid may be used as a selectable marker for expression of a protein of interest in host cells.Type: GrantFiled: September 21, 2007Date of Patent: March 20, 2012Assignee: Danisco US Inc.Inventors: Benjamin S. Bower, Nigel Dunn-Coleman, Nicholas Leiva
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Patent number: 8137925Abstract: The invention provides compositions and methods of use thereof for labeling peptide and proteins in vitro or in vivo. The methods described herein employ lipoic acid ligase or mutants thereof, and lipoic acid analogs recognized by lipoic acid ligase and lipoic acid ligase mutants.Type: GrantFiled: November 9, 2007Date of Patent: March 20, 2012Assignee: Massachusetts Institute of TechnologyInventors: Alice Y. Ting, Marta Fernandez Suarez, Hemanta Baruah
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Patent number: 8133705Abstract: The invention relates to a novel aminotransferase, DNA encoding the enzyme, a recombinant vector into which the DNA has been introduced, and a transformant into which the vector has been introduced. Further, the invention also relates to a method for producing an optically active amino compound utilizing the enzyme or transformant. The aminotransferase of the invention has an ability of efficiently converting a ketone compound, particularly a cyclic ketone compound to an optically active amino compound. According to the invention, a method for efficiently producing an optically active amino compound, particularly an optically active cyclic amino compound is provided.Type: GrantFiled: May 22, 2006Date of Patent: March 13, 2012Assignee: Kaneka CorporationInventors: Noriyuki Ito, Yoshihiko Yasohara
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Publication number: 20120058481Abstract: This specification generally relates to non-radioactive methods of non-radioactive real-time PCR using FRET dual-labeled primers.Type: ApplicationFiled: August 19, 2011Publication date: March 8, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Wayne Ge, Yue Ling Ng
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Publication number: 20120060242Abstract: The present invention pertains to the recombinant manufacture of polyunsaturated fatty acids. Specifically, it relates to acyltransferase polypeptides, polynucleotides encoding said acyltransferases as well as vectors, host cells, non-human transgenic organisms containing said polynucleotides. Moreover, the present invention contemplates methods for the manufacture of polyunsaturated fatty acids as well as oils obtained by such methods.Type: ApplicationFiled: May 11, 2010Publication date: March 8, 2012Applicant: BASF PLANT SCIENCE COMPANY GMBHInventors: Toralf Senger, Sten Stymne, Jenny Lindberg Yilmaz
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Publication number: 20120058527Abstract: Compositions and methods relating to lipase/acyltransferase enzymes identified in prokaryotes and eukaryotes are described. These enzymes can be used in such applications as lipid stain removal from fabrics and hard surfaces and chemical synthesis reactions.Type: ApplicationFiled: March 19, 2010Publication date: March 8, 2012Applicant: Danisco US Inc.Inventor: Susan Madrid
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Patent number: 8129155Abstract: A non-naturally occurring microbial organism having an isopropanol, 4-hydroxybutryate, or 1,4-butanediol pathway includes at least one exogenous nucleic acid encoding an isopropanol, 4-hydroxybutryate, or 1,4-butanediol pathway enzyme expressed in a sufficient amount to produce isopropanol, 4-hydroxybutryate, or 1,4-butanediol. The aforementioned organisms are cultured to produce isopropanol, 4-hydroxybutryate, or 1,4-butanediol.Type: GrantFiled: December 16, 2009Date of Patent: March 6, 2012Assignee: Genomatica, Inc.Inventors: John D. Trawick, Mark J. Burk, Anthony P. Burgard