Acting On Alpha-1, 4-glucosidic Bond, (e.g., Hyaluronidase, Invertase, Amylase, Etc. (some 3.2.1)) Patents (Class 435/201)
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Publication number: 20150079659Abstract: The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.Type: ApplicationFiled: May 22, 2014Publication date: March 19, 2015Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Stephen GORFIEN, Richard FIKE, GLENN GODWIN, JOYCE WOLANSKE, David EPSTEIN, Dale GRUBER, Don MCCLURE, Paul PRICE
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Patent number: 8980614Abstract: Methicillin-resistant (MRSA) and multi-drug resistant strains of Staphylococcus aureus are becoming increasingly prevalent in both human and veterinary clinics. S. aureus-causing bovine mastitis yields high annual losses to the dairy industry. Treatment of mastitis by broad range antibiotics is often not successful and may contribute to development of antibiotic resistance. Bacteriophage endolysins are a promising new source of antimicrobials. The endolysin of prophage ?SH2 of Staphylococcus haemolyticus strain JCSC1435 (?SH2 lysin) shows lytic activity against live staphylococcal cells. Deletion constructs were tested in zymograms and turbidity reduction assays to evaluate the contribution of each functional module to lysis. The CHAP domain exhibited three-fold higher activity than the full length protein. Activity was further enhanced in the presence of bivalent calcium ions.Type: GrantFiled: September 6, 2012Date of Patent: March 17, 2015Assignee: The United States of America, as represented by The Secretary of AgricultureInventors: David M. Donovan, Igor V. Abaev, Mathias Schmelcher
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Patent number: 8980578Abstract: The present invention provides fungal xylanase and/or beta-xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase and/or beta-xylosidases, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.Type: GrantFiled: June 10, 2013Date of Patent: March 17, 2015Assignee: Codexis, Inc.Inventors: Ryan Fong, Xiyun Zhang, Chris Noriega, Nicholas Agard, Anupam Gohel, Derek Smith
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Publication number: 20150072396Abstract: Low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations for modifying biomolecules is provided. Compositions, methods, and kits relating to low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations are also provided.Type: ApplicationFiled: March 1, 2012Publication date: March 12, 2015Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Kyle Gee, Upinder Singh, Scott Grecian, Scott Clarke
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Publication number: 20150072397Abstract: Polynucleotide sequences are provided encoding a thermostable cellulase and directing its increased expression are provided, and the use of the thermostable cellulase in hydraulic fracturing methods and the treatment of flowback fluids.Type: ApplicationFiled: March 12, 2013Publication date: March 12, 2015Inventors: Xuqiu Tan, Kenneth E. Barrett, Richard S. Lee
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Publication number: 20150064183Abstract: Targeted therapeutics that localize to a specific subcellular compartment such as the lysosome are provided. The targeted therapeutics include a therapeutic agent and a targeting moiety that binds a receptor on an exterior surface of the cell, permitting proper subcellular localization of the targeted therapeutic upon internalization of the receptor. Nucleic acids, cells, and methods relating to the practice of the invention are also provided.Type: ApplicationFiled: September 11, 2014Publication date: March 5, 2015Inventors: Jonathan LeBowitz, Stephen M. Beverley
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Publication number: 20150064157Abstract: The present invention provides further improved compositions and methods for efficient lysosomal targeting based on the GILT technology. Among other things, the present invention provides methods and compositions for targeting lysosomal enzymes to lysosomes using furin-resistant lysosomal targeting peptides. The present invention also provides methods and compositions for targeting lysosomal enzymes to lysosomes using a lysosomal targeting peptide that has reduced or diminished binding affinity for the insulin receptor.Type: ApplicationFiled: November 7, 2014Publication date: March 5, 2015Inventors: Jonathan H. LeBowitz, John Maga
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Patent number: 8962564Abstract: Described herein are variant, recombinant ?-glucocerebrosidase proteins characterized as having increased stability relative to recombinant wild-type ?-glucocerebrosidase. Also provided herein are variant, recombinant ?-glucocerebrosidase proteins characterized as retaining more catalytic activity relative to recombinant wild-type ?-glucocerebrosidase. Further described herein are variant, recombinant ?-glucocerebrosidase proteins that can have amino acid variations at one or more of the following positions: 316, 317, 321 and 145. Methods of making the variant, recombinant ?-glucocerebrosidase proteins are also described as well as methods of treating patients having lysosomal storage diseases.Type: GrantFiled: November 8, 2011Date of Patent: February 24, 2015Assignee: Amicus Therapeutics, Inc.Inventor: Hung Do
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Publication number: 20150037313Abstract: The present invention relates to a composition which comprises tryptophan whereby 10 to 90%, preferably 20 to 80% of the tryptophan is present as free tryptophan or peptide-bound tryptophan and 10 to 90%, preferably 20 to 80% of the tryptophan is present as polypeptide-bound tryptophan.Type: ApplicationFiled: July 10, 2014Publication date: February 5, 2015Inventors: Cinderella Christina GERHARDT, Luppo EDENS
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Publication number: 20150037842Abstract: The disclosure relates to a Gram negative bacterial cell that is transformed with a nucleic acid molecule that encodes a Gram positive twin-arginine translocase and including methods for the production of polypeptides.Type: ApplicationFiled: February 22, 2013Publication date: February 5, 2015Inventor: Colin Robinson
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Publication number: 20150031078Abstract: The present invention is a Kluyveromyces lactis yeast strain comprising the sequence identified by SEQ ID NO: 1, and methods for the production of sugars (glucose and galactose), ethanol, ?-galactosidase and biomass, in which said Kluyveromyces lactis yeast strain is cultured in the presence of a lactose-containing medium. The lactose-containing medium may be milk, whey, whey resulting from the preparation of butter, whey resulting after casein precipitation, milk permeate, whey permeate, acid whey and YPL culture medium.Type: ApplicationFiled: July 27, 2011Publication date: January 29, 2015Applicant: QUEIZUAR, S.L.Inventors: Angel Pereira Rodriguez, Manuel Becerra Fernandez, Maria Isabel Gonzalez Siso, Maria Esperanza Cerdan Villanueva
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Publication number: 20150031085Abstract: The present invention provides a novel leech HAase and a method of producing low-molecular-weight HA oligosaccharides using the leech HAase. This invention successfully cloned the first leech HAase gene and provides a method for high-level expression of the leech HAase gene. By controlling the incubation condition, different HA oligosaccharides, particularly HA4, HA6, HA8 and HA10, can be selectively generated using the leech HAase. The large-scale expression of the leech HAase and the enzymatic production of specific HA oligosaccharides are not only useful for the cosmetic, healthcare and the medical industries but also can be a great help to polysaccharides chemical synthesis and cancer research.Type: ApplicationFiled: April 12, 2014Publication date: January 29, 2015Applicant: JIANGNAN UNIVERSITYInventors: Jian Chen, Guocheng Du, Zhen Kang, Peng Jin
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Publication number: 20150031091Abstract: The present invention provides isolated polypeptides having alpha-amylase activity catalytic domains, carbohydrate binding domains and polynucleotide encoding the polypeptides, catalytic domains or carbohydrate binding domains. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding domains.Type: ApplicationFiled: September 29, 2012Publication date: January 29, 2015Applicant: NOVOZYMES A/SInventors: Ming Li, Junxin Duan, Noriko Tsutsumi, Guillermo Coward-Kelly, Henrik Lundkvist
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Publication number: 20150023944Abstract: A method for treating a Parkinson's patient with digestive/pancreatic enzymes involves administering an effective amount of digestive/pancreatic enzymes to an individual having the disorder in order to improve a symptom of the disorder. In addition, a method is provided for determining whether an individual has, or may develop, Parkinson's disease or related dysautonomic disorders and for determining whether an individual will benefit from the administration of pancreatic/digestive enzymes to treat the dysautonomic disorder.Type: ApplicationFiled: September 23, 2014Publication date: January 22, 2015Inventor: Joan M. Fallon
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Publication number: 20150024465Abstract: The present invention relates to non-catalytic carbohydrate-binding modules (CBM) belonging to a new family of CBM's. A CBM of the invention was found attached to a glycosyl hydrolase family 61 (GH61) polypeptide and was shown to have little homology with known CBM's indicating that it is the first known member of a new family of CBM's. The present invention further relates to CBM's preferably exhibiting binding affinity for cellulose; to a method of producing such CBM's; and to methods for using such CBM's in the textile, detergent and cellulose fiber processing industries, for purification of polypeptides, immobilisation of active enzymes, baking, manufacturing of biofuel, modification of plant cell walls.Type: ApplicationFiled: September 29, 2014Publication date: January 22, 2015Inventors: Kirk Matthew Schnorr, Lars Lehmann Hylling Christensen
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Publication number: 20150017685Abstract: The present invention relates to processes for the production of peptides, and the peptides produced accordingly. Peptides produced according to the invention may be produced more efficiently than peptides produced according to prior art processes. The production process of the invention may lead to advantages in yield, purity, and/or price. Methods of marketing peptides are also disclosed.Type: ApplicationFiled: July 8, 2014Publication date: January 15, 2015Inventors: Michael H. B. Stowell, Jonathan Caruthers, Travis Nemkov, Brian Hiester, Leslie Boux, Mikhail Plam
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Publication number: 20150017196Abstract: Described herein is the generation of optimized H5N1 and H1N1 influenza HA polypeptides for eliciting a broadly reactive immune response to influenza virus isolates. The optimized HA polypeptides were developed through a series of HA protein alignments, and subsequent generation of consensus sequences, based on H5N1 and H1N1 influenza isolates. Provided herein are optimized H5N1 and H1N1 influenza HA polypeptides, and compositions, fusion proteins and VLPs comprising the HA polypeptides. Further provided are codon-optimized nucleic acid sequences encoding the HA polypeptides. Methods of eliciting an immune response against influenza virus in a subject are also provided by the present disclosure.Type: ApplicationFiled: March 12, 2013Publication date: January 15, 2015Inventors: Ted M. Ross, Corey J. Crevar, Donald M. Carter
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Publication number: 20150010529Abstract: Modified PH20 hyaluronidase polypeptides that exhibit stability and activity under thermal stress conditions are provided. Also provided are compositions and formulations and uses thereof.Type: ApplicationFiled: July 3, 2014Publication date: January 8, 2015Inventor: Ge Wei
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Publication number: 20150004669Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce ethanol and/or butanol, e.g., by fermentation.Type: ApplicationFiled: September 11, 2014Publication date: January 1, 2015Inventor: Marshall Medoff
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Patent number: 8916366Abstract: The invention relates to a multi-cellulase enzyme composition for the enzymatic hydrolysis of cellulosic biomass said composition comprising a cellobiohydrolase (CBH) enzyme, an endoglucanase (EG) enzyme and a ?-glucosidase (BG) enzyme.Type: GrantFiled: November 18, 2010Date of Patent: December 23, 2014Assignee: Codexis, Inc.Inventors: Kripa Rao, Ish Dhawan, Sally Postlethwaite, Jie Yang
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Publication number: 20140370569Abstract: In a microbial fermentation, the aim is to increase the product yield of protein. This is achieved by a method in which an expression construct is introduced into a microorganism of the species Bacillus pumilus which comprises a promoter and a nucleic acid coding for the protein, and the protein is expressed in said expression construct.Type: ApplicationFiled: January 29, 2013Publication date: December 18, 2014Applicant: BASF SEInventors: Tobias Kueppers, Victoria Steffen, Renee Charlott Eichstadt, Stefan Evers, Karl-Heinz Maurer, Johannes Bongaerts, Hendrik Hellmuth, Thomas Weber, Timothy O'Connell
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Publication number: 20140373199Abstract: Provided are isolated polypeptides having beta-xylosidase activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 22, 2012Publication date: December 18, 2014Applicant: Novozymes Inc.Inventors: Yu Zhang, Ye Liu, Junxin Duan, Lan Tang, Brett McBrayer
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Publication number: 20140356914Abstract: The present invention provides endoglucanase 1b (EG1b) suitable for use in saccharification reactions.Type: ApplicationFiled: August 27, 2012Publication date: December 4, 2014Applicant: Codexis, Inc.Inventors: Kripa Rao, Xiyun Zhang, Brian R. Scott, John J. Tomashek
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Publication number: 20140356344Abstract: This disclosure relates to a method of generating conditionally active biologic proteins from wild type proteins, in particular therapeutic proteins, which are reversibly or irreversibly inactivated at the wild type normal physiological conditions. For example, evolved proteins are virtually inactive at body temperature, but are active at lower temperatures.Type: ApplicationFiled: August 20, 2014Publication date: December 4, 2014Applicant: BioAtla LLCInventors: Jay M. Short, Hwai Wen Chang, Gerhard Frey
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Publication number: 20140314735Abstract: The present invention comprises a method of concentrating a composition comprising a polypeptide of interest and the use of such a concentrated composition for the treatment of diseases in mammals, in particular by subcutaneous injection.Type: ApplicationFiled: July 1, 2014Publication date: October 23, 2014Inventor: Stefan NILSSON
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Patent number: 8865448Abstract: A novel microorganism is provided named Aspergillus saccharolyticus. Further, beta-glucosidase enzymes encoded by said microorganism are provided, and the use thereof in the degradation of lignocellulosic material. Also, host organisms comprising the polypeptides of the invention and/or polynucleotides encoding these are provided. In addition, methods, compositions, and kit-of-parts are provided which comprise any component of the invention, and optionally any additional components.Type: GrantFiled: August 1, 2011Date of Patent: October 21, 2014Assignee: Cleanvantage LLCInventors: Philip Teller, Peter S. Lubeck, Annette Sorensen, Birgitte K. Ahring
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Publication number: 20140302561Abstract: The present invention relates in particular to proteins, coding nucleic acid sequences for same, vectors comprising said coding sequences, a method for producing said proteins, and an oligosaccharide hydrolysis method using same. In particular, the invention relates to the protein of sequence SEQ ID no. 1. The present invention can be applied to the recycling of bio-natural resources formed by organisms and microorganisms including ulvans, in particular green algae.Type: ApplicationFiled: September 14, 2012Publication date: October 9, 2014Applicants: UNIVERSITÉ PIERRE ET MARIE CURIE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE- CNRS, CENTRE D'ÉTUDE ET DE VALORISATION DES ALGUESInventors: Pi Collen, William Helbert, Yannick Lerat, Jean-François Sassi
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Patent number: 8852584Abstract: The bacteriolytic effect of a bacterial cell wall lytic enzyme can be increased by the addition of a surfactant to the enzyme. As a result, the time required for lysis of cariogenic bacterium with the bacterial cell wall lytic enzyme can be shortened, and the practical utility of the bacterial cell wall lytic enzyme (such as automutanolysin) as a prophylactic or therapeutic agent for dental caries can be improved.Type: GrantFiled: July 25, 2006Date of Patent: October 7, 2014Assignee: Two Cells Co., Ltd.Inventors: Motoyuki Sugai, Hitoshi Komatsuzawa
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Publication number: 20140295522Abstract: The intention is to improve storage stability in a liquid washing or cleaning agent which contains a protease and amylase. This is achieved by using a protease which comprises an amino acid sequence which, over the entire length thereof, is at least 70% identical to the amino acid sequence stated in SEQ ID no. 1 and, in the numbering according to SEQ ID no. 1, has the amino acid substitution R99E or R99D in combination with at least two further amino acid substitutions which are selected from the group consisting of S3T, V4I and V199I.Type: ApplicationFiled: June 12, 2014Publication date: October 2, 2014Inventors: Nina Mussmann, Thomas Eiting, Thorsten Bastigkeit, Konstantin Benda, Hendrik Hellmuth
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Publication number: 20140295475Abstract: The application provides methods of producing a mixture of enzymes using two or more cell lines, methods of identifying or constructing cell lines for producing a mixture of enzymes, and methods of preparing a cell bank for producing a mixture of enzymes.Type: ApplicationFiled: December 4, 2012Publication date: October 2, 2014Applicant: DANISCO US INC.Inventors: George England, Suzanne Lantz
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Patent number: 8846865Abstract: The present invention relates to a polypeptide with an amino acid sequence according to SEQ ID NO: 1 and fragments or derivatives thereof. The present invention further relates to fusion proteins comprising said polypeptide and an additional peptide stretch fused to said polypeptide at the N- or C-terminus. Moreover, the present invention relates to nucleic acid molecules encoding said polypeptide or fusion protein, vectors comprising said nucleic acid molecules and host cells comprising either said nucleic acid molecules or said vectors. In addition, the present invention relates to said polypeptide or fusion protein for use as a medicament, in particular for the treatment or prevention of Gram-negative bacterial infections, as diagnostic means, as cosmetic substance or as sanitizing agent.Type: GrantFiled: August 24, 2010Date of Patent: September 30, 2014Assignees: Lysando AG, Katholieke Universiteit Leuven, K.U. Leuven R&DInventors: Yves Briers, Rob Lavigne, Maarten Walmagh, Stefan Miller
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Publication number: 20140286992Abstract: Influenza virus-like particles (VLPs) comprising influenza antigenic polypeptides are described. Also described are compositions comprising these VLPs as well as methods of making and using these VLPs.Type: ApplicationFiled: June 4, 2014Publication date: September 25, 2014Applicant: TechnoVax, Inc.Inventors: Jose M. Galarza, Demetrius Matassov
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Patent number: 8841091Abstract: The present invention relates to polypeptides comprising a carbohydrate-binding module amino acid sequence and an alpha-amylase amino acid sequence as well as to the application of such polypeptides.Type: GrantFiled: November 2, 2012Date of Patent: September 23, 2014Assignees: Novozymes Als, Novozymec North America Inc.Inventors: Shiro Fukuyama, Tomoko Matsui, Chee Leong Soong, Eric Allain, Anders Vikso Nielsen, Hiroaki Udagawa, Ye Liu, Junxin Duan, Wenping Wu, Lene Nonboe Andersen
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Publication number: 20140273150Abstract: Provided herein are compositions and methods that can remove, metabolize, or degrade a hydrocarbon in an area that is contaminated by hydrocarbons. Methods for bioremediation of an area such as an area of land, a body of water, or a shoreline that are contaminated by a hydrocarbon, such as from a crude oil spill are also described. The compositions and methods described herein can be used on natural flora and fauna as well as manmade materials that are contaminated by a hydrocarbon.Type: ApplicationFiled: July 23, 2013Publication date: September 18, 2014Inventor: Janet Angel
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Patent number: 8834865Abstract: The invention provides methods, compositions, systems, and kits that include an enzyme/substrate co-delivery system. The liquid delivery system includes at least one enzyme encapsulated in a water-soluble polymeric matrix and a substrate for the enzyme in a carrier liquid in which the polymeric matrix is insoluble. When water is added, the polymeric matrix is solubilized and enzyme is released from the matrix, permitting catalytic action upon the substrate.Type: GrantFiled: November 3, 2009Date of Patent: September 16, 2014Assignee: Danisco US Inc.Inventors: Nathaniel T. Becker, Michael Stoner, Mee-Young Yoon
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Publication number: 20140256000Abstract: The present invention provides isolated polypeptides having cellulolytic enhancing activity and polynucleotides encoding the polypeptides, catalytic domains, cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.Type: ApplicationFiled: October 31, 2012Publication date: September 11, 2014Inventors: Lan Tang, Ye Liu, Yu Zhang, Junxin Duan, Tarana Shaghasi
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Publication number: 20140227391Abstract: The present invention relates to isolated polypeptides having alpha-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: December 12, 2013Publication date: August 14, 2014Applicant: NOVOZYMES, INC.Inventors: Suzanne Otani, Haiyan Ge, Paul Harris, Debbie Yaver, Alexander Blinkovsky
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Publication number: 20140227741Abstract: Vaccines and therapeutic proteins, including polyclonal and monoclonal antibodies, must be maximally pure and stable in their most active native form. This is a requirement for their maximal efficacy, specificity and stability as well as for precluding immune responses against erroneous or damaged moieties. Similar considerations hold for proteins used in diagnostics, industry and research. The most frequent source of damage to proteins produced in living cells is the diverse product of oxidative damage. Two main sources of protein oxidation are the level of reactive oxygen species (ROS) and even more importantly the intrinsic susceptibility of proteins to oxidative damage. Methods for avoiding oxidative protein damage are disclosed, including providing for (i) a decrease in intracellular ROS levels and (ii) an increase in the intrinsic resilience of proteins to oxidative damage.Type: ApplicationFiled: December 18, 2013Publication date: August 14, 2014Applicant: Mediterranean Institute for Life SciencesInventors: Miroslav Radman, Anita Krisko
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Patent number: 8796005Abstract: A method for suppressing a reduction in an endoglucanase activity in the presence of a surfactant, characterized by modifying a protein having the endoglucanase activity in which the N-terminus is an amino acid other than pyroglutamic acid, to a protein having the N-terminus of pyroglutamic acid, is disclosed. Further, a modified protein having an endoglucanase activity wherein the N-terminal amino acid is converted into pyroglutamic acid by an amino acid modification, a polynucleotide encoding the protein, an expression vector comprising the polynucleotide, a host cell transformed with the expression vector, and a process for producing the protein by cultivating the host cell, are disclosed.Type: GrantFiled: September 19, 2013Date of Patent: August 5, 2014Assignee: Meiji Seika Pharma Co., Ltd.Inventors: Manabu Watanabe, Koji Yanai, Yumiko Tsuyuki
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Publication number: 20140206035Abstract: Provided are isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains.Type: ApplicationFiled: August 24, 2012Publication date: July 24, 2014Inventors: Tianqi Sun, Ming Li
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Patent number: 8772246Abstract: Provided are soluble neutral active Hyaluronidase Glycoproteins (sHASEGP's), methods of manufacture, and their use to facilitate administration of other molecules or to alleviate glycosaminoglycan associated pathologies. Minimally active polypeptide domains of the soluble, neutral active sHASEGP domains are described that include asparagine-linked sugar moieties required for a functional neutral active hyaluronidase domain. Included are modified amino-terminal leader peptides that enhance secretion of sHASEGP. Sialated and pegylated forms of the sHASEGPs also are provided. Methods of treatment by administering sHASEGPs and modified forms thereof also are provided.Type: GrantFiled: October 18, 2012Date of Patent: July 8, 2014Assignee: Halozyme, Inc.Inventors: Louis H. Bookbinder, Anirban Kundu, Gregory I. Frost
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Publication number: 20140186326Abstract: A modified human acid alpha-glucosidase polypeptide is provided, as well as methods of making and using modified human acid alpha-glucosidase to treat glycogen storage disorders.Type: ApplicationFiled: April 20, 2012Publication date: July 3, 2014Inventors: William Canfield, Mariko Kudo, Rodney Moreland
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Patent number: 8765685Abstract: Provided are soluble neutral active Hyaluronidase Glycoproteins (sHASEGP's), methods of manufacture, and their use to facilitate administration of other molecules or to alleviate glycosaminoglycan associated pathologies. Minimally active polypeptide domains of the soluble, neutral active sHASEGP domains are described that include asparagine-linked sugar moieties required for a functional neutral active hyaluronidase domain. Included are modified amino-terminal leader peptides that enhance secretion of sHASEGP. Sialated and pegylated forms of the sHASEGPs also are provided. Methods of treatment by administering sHASEGPs and modified forms thereof also are provided.Type: GrantFiled: May 16, 2012Date of Patent: July 1, 2014Assignee: Halozyme, Inc.Inventors: Louis Bookbinder, Anirban Kundu, Gregory I. Frost
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Publication number: 20140178330Abstract: Modified PH20 hyaluronidase polypeptides, including modified polypeptides that exhibit increased stability and/or increased activity, are provided. Also provided are compositions and formulations and uses thereof.Type: ApplicationFiled: December 28, 2012Publication date: June 26, 2014Inventors: Ge Wei, H. Michael Shepard, Qiping Zhao, Robert James Connor
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Patent number: 8759039Abstract: The present invention relates to the isolation and to the characterization of two proteins having a novel enzymatic activity, i.e. a porphyranase activity. These proteins are useful for hydrolyzing polysaccharides containing sulfated agaro-colloids and for producing oligo-porphyrans, notably oligo-porphyrans with a defined structure and size.Type: GrantFiled: July 20, 2010Date of Patent: June 24, 2014Assignee: Centre National de la Recherche Scientifique (C.N.R.S.)Inventors: Jan-Hendrik Hehemann, Gaëlle Correc, Gurvan Michel, Tristan Barbeyron, William Helbert, Mirjam Czjzek
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Patent number: 8753859Abstract: This invention relates to polypeptides, more specifically amylase polypeptides and nucleic acids encoding these, and their uses e.g. as non-maltogenic exoamylases in producing food or feed products.Type: GrantFiled: November 2, 2011Date of Patent: June 17, 2014Assignee: Dupont Nutrition Biosciences APSInventors: Karsten Matthias Kragh, Anja Hemmingsen Kellet-Smith, René Mikkelsen, Rie Mejldal, Rikke L. Bundgaard Jenner
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Publication number: 20140154707Abstract: Transformed Phaeodactylum tricornutum including a nucleic acid sequence operatively linked to a promoter, wherein the nucleic acid sequence encodes an N-acetylglucosaminyltransferase I and/or an ?-Mannosidase II and wherein at least one ?-N-acetylglucosaminidase of the transformed Phaeodactylum tricornutum has been inactivated. A method for producing a glycosylated polypeptide includes the steps of (i) culturing a transformed P. tricornutum as defined previously and (ii) purifying the polypeptide that is expressed and glycosylated in the transformed P. Tricornutum. The use of such a transformed P. tricornutum for producing a glycosylated polypeptide is also described.Type: ApplicationFiled: July 27, 2011Publication date: June 5, 2014Applicants: UNIVERSITE DE ROUEN, L'INSTITUT FRANCAIS DE RECHERCHE POUR L'EXPLOITATION DE LA MER (IFREMER), ALGENICSInventors: Muriel Bardor, Romain Louvet, Bruno Saint-Jean, Carole Burel, Berangere Baiet, Jean-Paul Cadoret, Patrice Lerouge, Remy Michel, Aude Carlier
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Patent number: 8735130Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: November 30, 2010Date of Patent: May 27, 2014Assignees: Novozymes A/S, Novozymes North America, Inc.Inventors: Sara Landvik, Keiichi Ayabe, Guillermo Coward-Kelly
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Patent number: 8735137Abstract: Disclosed herein are transformed Yarrowia lipolytica comprising an exogenous polynucleotide encoding a polypeptide having sucrose invertase activity. Also disclosed are methods of using the transformed Y. lipolytica.Type: GrantFiled: November 15, 2011Date of Patent: May 27, 2014Assignee: E I du Pont de Nemours and CompanyInventors: Seung-Pyo Hong, John E. Seip, Quinn Qun Zhu
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Publication number: 20140141473Abstract: Yeast cell belonging to the genus Saccharomyces having introduced into its genome at least one xylA gene and at least one of each of araA, araB and araD genes and that is capable of consuming a mixed sugar mixture comprising glucose, xylose and arabinose, wherein the cell co-consumes glucose and arabinose, has genetic variations obtained during adaptive evolution and has a specific xylose consumption rate in the presence of glucose that is 0.25 g xylose/h, g DM or more.Type: ApplicationFiled: April 20, 2012Publication date: May 22, 2014Applicant: DSM IP ASSETS B.V.Inventors: Paul Klaassen, Bianca Elisabeth Maria Gielesen, Gijsberdina Pieternella Van Suylekom, Panagiotis Sarantinopoulos, Wilbert Herman Marie Heijne, Aldo Greeve